Whitening composition and application thereof

Abstract

The invention belongs to the field of cosmetics, and discloses a whitening composition and application thereof. The composition comprises the following specific components in percentage by weight: 80-90% of honey extract, 5-10% of papain and 5-10% of lactobacillus fermentation lysate. The composition can mildly promote cells containing melanin on the skin cuticle to fall off, accelerate cell metabolism of the cuticle, achieve the effects of whitening and smoothing the skin and meet the requirements of people for whitening and not damaging the skin barrier. Meanwhile, the invention further provides application and cosmetics of the composition.

Description

Technical Field

[0001] The present invention relates to the field of cosmetics, and particularly to a whitening composition and its application. Background Art

[0002] Under normal circumstances, the epidermal cells of human skin maintain a dynamic balance by continuously dividing and evolving, and the old or damaged keratinocytes naturally exfoliate. When the barrier function is affected by external factors such as ultraviolet radiation, trauma, and over-cleaning, it will cause skin tanning, uneven skin color, and rough skin surface, thus affecting the beauty of appearance. The normal epidermal renewal time of the skin is about 28 days. If the old keratinocytes fail to exfoliate normally and accumulate on the skin surface, it will cause the epidermis to thicken, and the keratinocytes containing melanin fail to exfoliate in time, resulting in deepening and dulling of the skin color, and even the formation of skin spots or tanning.

[0003] There are mainly two methods for achieving skin rejuvenation and whitening by exfoliation on the market. One is the physical exfoliation method, which usually uses physical abrasive particles to rub the skin to remove aging keratinocytes and accelerate the metabolism and renewal of new keratinocytes. Physical exfoliation may easily lead to excessive keratin cleaning if used improperly, making the skin more sensitive and even causing mechanical damage to the skin. The other is the chemical exfoliation method, which softens the stratum corneum by applying fruit acids such as tartaric acid, malic acid, lactic acid, citric acid, glucuronic acid, and salicylic acid, reduces the connection force between keratinocytes, and causes the aging cells containing melanin on the skin surface to exfoliate, thus achieving the effect of smooth and fair skin. However, these fruit acids usually have a low pH and high irritation. Over-brushing with fruit acids will damage the skin barrier, resulting in dry and red skin, and is not suitable for sensitive skin. Therefore, it is an urgent problem to be solved to develop a composition that can promote the renewal and exfoliation of melanin-containing keratinocytes on the skin while being mild and non-irritating.

[0004] Therefore, the technical problem to be solved in this case is: how to develop a new whitening composition that can accelerate keratin renewal without harming the skin. Summary of the Invention

[0005] To solve the above problems, the present invention provides a whitening composition.

[0006] One of the purposes of the present invention is to provide a whitening composition to solve the technical problem of high irritation of most exfoliating products on the market.

[0007] Another purpose of the present invention is to provide an application of a whitening composition. Applying this composition to skin care products helps to promote the metabolism of stratum corneum cells of the skin, promote the exfoliation of aging cells, whiten and fade skin color, and make the skin smooth and flat.

[0008] To achieve the above object, the present invention adopts the following technical solutions:

[0009] A whitening composition, the whitening composition comprising the following components: honey extract solution, papain solution, Lactobacillus ferment lysate solution;

[0010] The honey extract solution contains 14wt% - 18wt% of honey extract;

[0011] The papain solution contains 6wt% - 10wt% of papain;

[0012] The Lactobacillus ferment lysate solution contains 1wt% - 5wt% of Lactobacillus ferment lysate.

[0013] Preferably, the honey extract solution is composed of 14wt% - 18wt% of honey extract, 15wt% - 25wt% of 1,3 - propanediol, and the balance of water;

[0014] The papain solution is composed of 6wt% - 10wt% of papain, 55wt% - 65wt% of glycerol, and the balance of water;

[0015] The Lactobacillus ferment lysate solution is composed of 1wt% - 5wt% of Lactobacillus ferment lysate, 25wt% - 35wt% of 1,3 - propanediol, 2wt% - 6wt% of 1,2 - pentanediol, 0.05wt% - 0.1wt% of octanoyl hydroxamic acid, and the balance of water.

[0016] In the present invention, through the synergistic effect of honey extract, papain, and Lactobacillus ferment lysate: honey extract degrades the keratinocyte desmosomes, and papain enzymatically hydrolyzes keratin. The two act together on the aged keratinocytes, significantly reducing the keratinocyte connection ability, thereby achieving the effect of keratinocyte metabolism. Lactobacillus ferment lysate maintains the acidic environment of the skin, supports the biological activities of honey extract and papain, and Lactobacillus ferment lysate has an antioxidant effect, capable of scavenging DPPH and peroxidized lipids, achieving the effect of brightening the skin. Therefore, the synergistic effect of honey extract, papain, and Lactobacillus ferment lysate can significantly promote the metabolism of the skin stratum corneum cells, exfoliate the aged cells, whiten and fade the skin color, make the skin smooth and flat, and do not harm the skin.

[0017] Preferably, the whitening composition, by weight percentage, comprises: 80% - 90% of honey extract solution, 5% - 10% of papain solution, and 5% - 10% of Lactobacillus ferment lysate solution.

[0018] More preferably, the whitening composition comprises, by weight percentage: 83% to 87% of honey extract solution, 6% to 8% of papain solution, and 6% to 8% of Lactobacillus ferment lysate solution.

[0019] In addition, the present invention also discloses a use of the whitening composition for preparing skin care products.

[0020] Preferably, the content of the whitening composition in the skin care products is 1 wt% to 2 wt%.

[0021] Finally, the present invention also discloses a skin care product containing the whitening composition as described above.

[0022] Preferably, the skin care products include lotion, cream, spray, essence, essential oil, facial cleanser, lotion, facial mask, gel.

[0023] In other studies of the present invention, it was found that even samples obtained by using a single component or a combination of two components of honey extract / papain / Lactobacillus ferment lysate also have the effect of promoting the renewal of skin stratum corneum cells, but their efficacy in keratin renewal is poor and the irritation to the skin is relatively large.

[0024] The present invention is composed of three active substances: honey extract, papain, and Lactobacillus ferment lysate.

[0025] The honey extract is obtained by natural fermentation extraction, contains a high concentration of gluconic acid. Compared with strongly irritating acids such as glycolic acid, lactic acid, malic acid, and citric acid, the skin rejuvenation and whitening effect of the honey extract is more gentle. The gluconic acid contained in the honey extract can activate cathepsin to degrade the desmosomes connecting keratinocytes, achieving efficient keratin renewal and reducing the distribution of melanin in the stratum corneum. Due to its polyhydroxy structure, gluconic acid has a strong attraction to water, so its moisturizing property is greatly improved. The honey extract can be used in a wide range of pH values and still maintain the keratin renewal effect in the neutral range.

[0026] Papain is a cysteine protease extracted from Carica papaya, with characteristics such as high enzyme activity, good thermal stability, natural hygiene and safety. Papain has a strong hydrolysis ability for animal and plant proteins, polypeptides, esters, amides, etc. Acting on the skin stratum corneum, it dissolves keratin proteins, reduces the connection between keratinized desmosomes, thereby gently and effectively exfoliating the cutin, improving skin dullness, and making the skin fair and delicate.

[0027] The cell lysate of Lactobacillus fermentation contains components such as organic acids, amino acids, vitamins, polysaccharides, small molecular peptides, and biological enzymes. The organic acids therein can balance the pH of the skin surface, making the pH of the skin weakly acidic; the amino acids, vitamins, and biological enzymes contained can repair epidermal cells, remove pigment deposits, improve skin roughness, and make the skin bright and elastic; the microbial polysaccharides contained in the cell lysate of Lactobacillus fermentation also have antioxidant activity, and both neutral polysaccharides and acidic polysaccharides can effectively inhibit lipid peroxidation and strongly scavenge DPPH, OH, and peroxides.

[0028] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0029] 1. The present invention uses a compound of three active substances, namely honey extract, papain, and cell lysate of Lactobacillus fermentation, to synergistically achieve the effects of cutin renewal and reduction of melanin distribution. The cell lysate of Lactobacillus fermentation can provide a more stable pH environment, which is more conducive to maintaining the structure and function of papain. The honey extract can also play its role when stabilized at a stable pH. Therefore, there is no incompatibility among the three.

[0030] 2. The present invention mainly uses this natural extract of honey extract, supplemented with components such as papain and cell lysate of Lactobacillus fermentation. While ensuring its cutin renewal and whitening effects, it also has high mildness. Specific Embodiments

[0031] Next, the technical solutions of the present invention will be clearly and completely described in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without making creative efforts belong to the scope of protection of the present invention.

[0032] The source information of each raw material in the examples and comparative examples is as follows:

[0033] Papain solution: Guangzhou Sainalong Fine Chemical Co., Ltd., containing 60 wt% glycerol, 32 wt% water, 8 wt% papain, and enzyme activity (U / ml) ≥ 80000;

[0034] Honey extract solution: Guangzhou Hengtao Trading Co., Ltd., containing 64 wt% water, 20 wt% 1,3 - propanediol, and 20 wt% honey extract;

[0035] Cell lysate of Lactobacillus fermentation solution: Bestlife Biotechnology (Zhejiang) Co., Ltd., containing 3 wt% cell lysate of Lactobacillus fermentation, 30 wt% 1,3 - propanediol, 4 wt% 1,2 - pentanediol, 0.075 wt% octanoyl hydroxamic acid, and 65.925 wt% water;

[0036] Lactic acid: Guangzhou Tinglan Biotechnology Co., Ltd., specific rotation (20 °C, D) is +23°--+29°, molecular weight is 358.296 g / mol;

[0037] Bromelain: Nanning Pangbo Bioengineering Co., Ltd., concentration range is 500,000 - 1,200,000 u / g, and the bromelain concentration used in the comparative example is 800,000 u / g;

[0038] Streptococcus thermophilus fermentation product solution: Guangzhou Youran Biotechnology Co., Ltd., containing 10 wt% of Streptococcus thermophilus fermentation product, 2 wt% of 1,2-ethylene glycol, 1 wt% of 1,2-pentanediol, and 87 wt% of water;

[0039] Overview of the production process of the papain described above: water immersion, filtration, glycerol extraction, concentration, formulation, inspection, packaging, and after passing the inspection, it enters the qualified product warehouse;

[0040] Overview of the production process of the honey extract solution described above: honey refining, fermentation, heating (inactivation and sterilization), cooling, adding the co-preservative solvent 1,3-propanediol, and filtration and sterilization;

[0041] Overview of the production process of the Lactobacillus ferment lysate solution described above: seed activation, medium sterilization, inoculation, fermentation, end of fermentation, centrifugation, collection of bacterial cells, high-pressure crushing, centrifugation, membrane filtration, addition of preservatives, inspection, and finished product;

[0042] Overview of the production process of the lactic acid described above: lactose is dissolved in distilled water, Au / C (activated carbon) catalyst is added, stirred, oxygen is introduced, the pH is adjusted and kept constant with sodium hydroxide, filtered through cation exchange resin, concentrated, cooled and crystallized, inspected, and stored in the warehouse;

[0043] Overview of the production process of the Streptococcus thermophilus fermentation product solution described above: fermentation, crushing, purification, and sub-packaging;

[0044] It should be noted that the technical solution of the present invention can be realized by other commercially available products, not limited to the extracts provided by the above manufacturers only.

[0045] In the following examples and comparative examples, unless otherwise specified, the parts are parts by weight, and the % is weight percentage.

[0046] Example 1

[0047] A whitening composition, comprising the following components in parts by weight: 90 parts of honey extract solution, 5 parts of papain solution, and 5 parts of Lactobacillus ferment lysate solution.

[0048] Example 2

[0049] A whitening composition, comprising the following components in parts by weight: 85 parts of honey extract solution, 10 parts of papain solution, and 5 parts of Lactobacillus ferment lysate solution.

[0050] Example 3

[0051] A whitening composition, comprising the following components in parts by weight: 85 parts of honey extract solution, 7.5 parts of papain solution, and 7.5 parts of Lactobacillus ferment lysate solution.

[0052] Example 4

[0053] A whitening composition, comprising the following components in parts by weight: 85 parts of honey extract solution, 5 parts of papain solution, and 10 parts of Lactobacillus ferment lysate solution.

[0054] Example 5

[0055] A whitening composition, comprising the following components in parts by weight: 80 parts of honey extract solution, 10 parts of papain solution, and 10 parts of Lactobacillus ferment lysate solution.

[0056] Comparative Example 1

[0057] A whitening composition, comprising the following components in parts by weight: 50 parts of papain solution and 50 parts of Lactobacillus ferment lysate solution.

[0058] Comparative Example 2

[0059] A whitening composition, comprising the following components in parts by weight: 90 parts of honey extract solution and 10 parts of Lactobacillus ferment lysate solution.

[0060] Comparative Example 3

[0061] A whitening composition, comprising the following components in parts by weight: 90 parts of honey extract solution and 10 parts of papain solution.

[0062] Comparative Example 4

[0063] A composition, consisting of 85 parts of lactic acid, 7.5 parts of papain solution, and 7.5 parts of Lactobacillus ferment lysate solution.

[0064] Comparative Example 5

[0065] A composition, 85 parts of honey extract solution, 7.5 parts of bromelain, and 7.5 parts of Lactobacillus ferment lysate solution.

[0066] Comparative Example 6

[0067] A composition, 85 parts of honey extract solution, 7.5 parts of papain solution, and 7.5 parts of Streptococcus thermophilus fermentation product solution.

[0068] Performance Detection

[0069] 1. Exfoliating Efficacy Test

[0070] Cuticle Staining Model Test Method

[0071] Detection Principle: Diacetone alcohol (DHA) staining is adopted. The functional groups in DHA can react with the amino acids and amino groups of skin keratin to form brown polymers, making the skin produce an artificial brown color (Maillard reaction). This reaction is only based on the cuticle and will not affect the skin structure deeper than the cuticle. Therefore, it is used to simulate the staining of the cuticle on the skin surface. The exfoliating effect of the product is evaluated by the change degree of the color difference value after cuticle staining.

[0072] Specific Test Method: 33 healthy subjects aged 18 - 60 are selected, and there are no scar, pigmentation, erythema and other factors affecting the results in the test areas of the subjects' arms. Use Skin-Colorimeter CL400 to measure the initial value of ITA° in 4 test areas. Each test area is measured in parallel 3 times, and the average value is taken (subjects with the initial skin color ITA° > 20° in the test area shall not be included in the test). Stain the test areas with dihydroxyacetone (DHA). 48 hours after staining, start to measure the ITA° value on the 0th day by using Skin-Colorimeter CL400 type skin color tester. Each test area is measured in parallel 3 times, and the average value is taken. Starting from the 0th day, apply the corresponding example samples to each stained test area. Each test substance is evenly applied to the test area at a dosage of (2.00 ± 0.05) mg / cm 2 and the dosage is evenly applied to the test area 2 times a day, and the interval time between each application is not less than 4 h. The test period is 14 days. Collect the ITA° values of each test on the 0th, 3rd, 7th, and 14th days at the start of the formal test. Each test area is measured in parallel 3 times, and the average value is taken. The larger the ITA° value, the lighter the skin color, the more cutin metabolism, and the better the whitening and brightening effect. On the contrary, the darker the skin color, the slower the cutin renewal rate.

[0073] Calculation of Cutin Renewal Rate

[0074] Data Analysis

[0075] The statistical analysis software is SPSS. The independent samples t-test is used for the comparison between the test samples and the negative control; the above statistical analyses are all two-tailed tests, and the significance level is α = 0.05; P > 0.05 indicates that there is no significant difference between the two groups; P < 0.05 indicates that there is a significant difference between the two groups.

[0076] The compositions prepared in Examples 1 - 5 and Comparative Examples 1 - 6 are tested according to the above experiment, and the results are shown in Table 1;

[0077] Table 1 Measurement results of ITA° values of the compositions prepared in Examples 1-5 and Comparative Examples 1-6

[0078]

[0079] Note: * indicates significant difference compared with the blank control group, P < 0.05.

[0080] According to the results in Table 1, it can be seen that:

[0081] After using for 3 days and 7 days, there was no significant difference among groups compared with the blank control group. According to the comparison with the blank control group, the ITA° of Examples 1-5 after using for 14 days had significant differences (P < 0.05). It can be concluded that the composition scheme of honey extract, papain, and Lactobacillus ferment lysate used in the present invention has an obvious exfoliating effect and can significantly whiten and brighten the skin tone. By comparing the ITA° value data of Example 1 and Comparative Example 1 on the 14th day, it can be shown that honey extract can significantly whiten and brighten the skin tone and promote the metabolism of keratinocytes. Its mechanism is to degrade the desmosomes connecting keratinocytes, making the structure of aging cells less compact and easier to exfoliate. According to the 14-day ITA° value data of Comparative Examples 2-3, when honey extract was retained and paired with one of papain and Lactobacillus ferment lysate, the effect of promoting keratinocyte metabolism decreased significantly. This is because papain has a high hydrolysis effect on the proteins in the stratum corneum, softening the keratinocyte cytoskeleton - cytokeratin and dissolving it; the components such as bioenzymes, organic acids, and polysaccharides contained in Lactobacillus ferment lysate accelerate the metabolism of pigment cells, reduce pigment accumulation, and provide a beneficial pH environment for honey extract and papain, helping to maintain the activity of the former two and enhancing the keratin renewal effect of the former two. The above shows that the composition of the present invention is obtained by compounding three components, namely honey extract, papain, and Lactobacillus ferment lysate, to synergistically promote keratinocyte metabolism. The three work together synergistically and are indispensable.

[0082] According to the data comparison between Example 3 and Comparative Examples 4-6, in Comparative Example 4, lactic acid was used to replace the honey extract solution, in Comparative Example 5, bromelain was used to replace the papain solution, and in Comparative Example 6, Streptococcus thermophilus fermentation product solution was used to replace the Lactobacillus ferment lysate solution. All of them had a certain effect on promoting the metabolism of the stratum corneum, but their effects were far weaker than those of Example 3. It can be reasonably speculated that the compounding of honey extract, papain, and Lactobacillus ferment lysate in the present invention has a synergistic effect on improving keratinocyte metabolism.

[0083] In order to further evaluate the performance differences of the above products, in the stratum corneum staining model test method, the tested concentration of the test substance for the volunteers was increased;

[0084] The specific method is as follows: increase the concentration of the test substance to (4.00 ± 0.05) mg / cm 2 , and conduct the test again; the test results can be referred to Table 2 below;

[0085] Table 2 Measurement results of ITA° values of the compositions prepared in Examples 1-5 and Comparative Examples 1-6

[0086]

[0087]

[0088] It can be seen from the above experimental data that when the concentration increases, the performance of each comparative example has been significantly improved, and the performance of each example has been improved to a certain extent.

[0089] The above experiments can illustrate that the combination of the honey extract solution, papain solution, and Lactobacillus ferment lysate solution of the present invention can effectively reduce the usage of the active ingredients and make the composition exert a more significant effect.

[0090] 2. Whitening efficacy test

[0091] Test method: Refer to the test method for the freckle-removing and whitening efficacy of cosmetics (the first method). Select 35 subjects aged 18-60 without pigmentation, inflammation, scars, etc. on the back skin that affect the test results. Use a solar simulator to build a skin darkening model for the test area. On the day when the modeling is completed, use an instrument to detect the ITA° and melanin content of the skin color in each test area. The subjects start to use the sample once in the morning and once in the evening in each darkened test area for 4 consecutive weeks. At the 4th week, collect the data of ITA° and melanin content.

[0092] Sample preparation: Prepare essence liquids containing different whitening compositions of Example 4 and their application contents according to Table 3.

[0093] Table 3 Formulation table of essence liquids with different contents

[0094]

[0095] Test samples 1-7 according to the above whitening efficacy test method, and the results are shown in Tables 4 and 5;

[0096] Table 4 Comparison results of ITA° of test samples

[0097]

[0098]

[0099] Table 5 Comparison results of MI values of test samples

[0100]

[0101] As can be seen from Table 4 and Table 5,

[0102] Compared with Sample 1 (negative control), Samples 2 - 7 all showed the effects of brightening the skin and reducing the melanin content; Sample 2 had a certain brightening effect but no significant difference (P > 0.05), and there were significant differences in ITA° and MI of Samples 3 - 7 (P < 0.05). As the content of the composition in Example 4 increased, its whitening effect enhanced accordingly; among them, the enhancement of the effects of Samples 6 - 7 tended to level off and there was no obvious improvement compared with Sample 5. The above whitening efficacy test experiments showed that the preferred usage amount of the whitening composition provided by the present invention in skin care products was 1 - 2 wt%, the product efficacy was not obvious when it was lower than 1 wt%, and the product efficacy did not increase significantly when it was higher than 2 wt%.

[0103] 3. Gentleness test

[0104] Patch test

[0105] Specific test method: Screen 34 volunteers aged 18 - 60 who met the test requirements as the test subjects. Gently wipe the test area with a dry soft tissue paper. Take 0.025 g of the sample to be tested and add it to the patch tester. According to the preset sample position, stick the patch tester with the corresponding number on the corresponding test area. Quickly stick the patch tester from top to bottom to the corresponding position, gently press and smooth it, and apply it to the skin for 24 h. Then, the tester removes the patch tester and marks it with a skin marker pen. Record the results observed with the naked eye 0.5 h after removing the patch (after the indentation disappears).

[0106] Judgment criteria: Skin reaction grading criteria for skin closed patch test: Grade 0: Negative reaction, no adverse reaction; Grade 1: Suspected reaction, only faint erythema; Grade 2: Weak positive reaction (erythema reaction); erythema, infiltration, edema, and there may be papules; Grade 3: Strong positive reaction (herpes reaction); erythema, infiltration, edema, papules, herpes; the reaction can exceed the test area; Grade 4: Extremely strong positive reaction (confluent herpes reaction); obvious erythema, severe infiltration, edema, confluent herpes; the reaction exceeds the test area.

[0107] Perform skin patch tests on the examples according to the above test method, and the results are shown in Table 6;

[0108] Table 6 Skin patch test results

[0109]

[0110] From the statistical results of the patch test, it can be concluded that no patch positive reactions occurred in Examples 1-5 and Comparative Examples 1-3, that is, the combination of honey extract, papain and lysed product of Lactobacillus fermentation has little skin irritation and high safety.

[0111] For those skilled in the art, it is obvious that the present invention is not limited to the details of the above exemplary embodiments, and the present invention can be implemented in other specific forms without departing from the spirit or basic characteristics of the present invention. Therefore, from any point of view, the embodiments should be regarded as exemplary and non-limiting. The scope of the present invention is defined by the appended claims rather than the above description. Therefore, all changes falling within the meaning and scope of the equivalent elements of the claims are intended to be included in the present invention.

Claims

1. A whitening composition, characterized in that, By weight percentage, the whitening composition comprises the following components: 80 wt% to 90 wt% of honey extract solution, 5 wt% to 10 wt% of papain solution, and 5 wt% to 10 wt% of Lactobacillus ferment lysate solution; The honey extract solution contains 14 wt% to 18 wt% of honey extract; The papain solution contains 6 wt% to 10 wt% of papain; The Lactobacillus ferment lysate solution contains 1 wt% to 5 wt% of Lactobacillus ferment lysate.

2. The whitening composition according to claim 1, wherein The honey extract solution is composed of 14 wt% to 18 wt% of honey extract, 15 wt% to 25 wt% of 1,3-propanediol, and the balance of water; The papain solution is composed of 6 wt% to 10 wt% of papain, 55 wt% to 65 wt% of glycerol, and the balance of water; The Lactobacillus ferment lysate solution is composed of 1 wt% to 5 wt% of Lactobacillus ferment lysate, 25 wt% to 35 wt% of 1,3-propanediol, 2 wt% to 6 wt% of 1,2-pentanediol, 0.05 wt% to 0.1 wt% of octanoyl hydroxamic acid, and the balance of water.

3. Use of the whitening composition according to claim 1 or 2 for preparing skin care products.

4. The use according to claim 3, characterized in that, The skin care products are skin care products for whitening.

5. A skin care product, characterized in that, Containing the whitening composition according to claim 1 or 2.

6. The skin care product according to claim 5, wherein The skin care products contain 1 wt% to 2 wt% of the whitening composition.

7. The skin care product according to claim 6, characterized in that, The skin care products include lotion, cream, spray, essence, essential oil, facial cleanser, lotion, facial mask, gel.

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