Organic fertilizer added with abamectin mushroom dregs

Abstract

The invention relates to the field of fertilizers, in particular to an organic fertilizer added with abamectin mushroom dregs, which is obtained by mixing modified abamectin mushroom dregs with a microbial composite inoculant, compounding with a phosphate fertilizer and a potash fertilizer and granulating. The organic fertilizer added with the abamectin fungus residues is prepared by modifying the existing abamectin fungus residues, compounding the modified abamectin fungus residues with a microbial compound inoculant, and mixing the compounded product with a phosphate fertilizer and a potash fertilizer. And the prepared organic fertilizer has a good effect of improving the yield of crops.

Description

Technical Field

[0001] The present invention relates to the field of fertilizers, and specifically relates to an organic fertilizer added with avermectin bacterial residue. Background Art

[0002] Antibiotic bacterial residue is the solid waste generated during the production of antibiotics. Its main components are the mycelia of antibiotic-producing bacteria, the unused culture medium, the metabolites produced during fermentation, the degradation products of the culture medium, and a small amount of antibiotics, etc. The existing antibiotic bacterial residues mainly include tetracyclines, aminoglycosides, polypeptides, macrolides, antifungal agents, antituberculosis agents, etc. Because they contain quite a lot of nutrients, the past treatment method of antibiotic bacterial residue was to dry it and use it as feed or feed additive. Due to the residual small amount of antibiotics in it, if animals eat it for a long time, it will cause problems such as drug resistance and zoonoses. The country has paid increasing attention to the removal of antibiotics in feed and included it in the "List of Drug Varieties Prohibited from Use in Feed and Animal Drinking Water" in 2002.

[0003] The treatment methods of antibiotic bacterial residue mainly include landfill, incineration, anaerobic digestion, and composting treatment, etc. Due to the characteristics of large output, high water content, and high nutrient content of antibiotic bacterial residue, if it is directly landfilled safely, it will occupy a large area, have a high disposal cost, and easily cause waste of resources; for incineration and pyrolysis, prior drying treatment or additional fuel addition is required, with high operating costs, and it is extremely easy to cause secondary pollution and lead to the residues of heavy metals and antibiotics; in the anaerobic digestion system, the amount of pollutants generated is relatively large and the wastewater is difficult to treat; the aerobic composting method can achieve the harmless treatment of bacterial residue and develop special fertilizers to realize the resource utilization of bacterial residue, but the application of this method in practice still has limitations, with a long time and difficult to control, and the existing aerobic composting technology does not completely degrade the antibiotics in the bacterial residue, and there are still small amounts of antibiotic residues, which have a certain impact on the environment. Summary of the Invention

[0004] Aiming at the problems existing in the prior art, the purpose of the present invention is to provide an organic fertilizer added with avermectin bacterial residue.

[0005] The purpose of the present invention is achieved by the following technical solutions: An organic fertilizer added with avermectin bacterial residue is obtained by mixing modified avermectin bacterial residue with a microbial complex bactericide, and then compounding with phosphate fertilizer and potassium fertilizer and granulating.

[0006] Preferably, the preparation method of the modified avermectin bacterial residue includes the following steps: Step (1), after drying and treating the avermectin bacterial residue, grinding and sieving it, and then placing it in an alkali solution, first introducing ozone for treatment, removing ozone and then raising the temperature for stirring treatment, and then drying again to obtain alkali-treated avermectin bacterial residue powder; Step (2): Mix the alkali-treated avermectin residue powder with an organic solvent. After stirring well, add tris(2-carboxyethyl)isocyanurate, dropwise add a catalyst, heat up to 70 - 100 °C, keep stirring for 2 - 4 h. After the reaction is completed, remove the solvent under reduced pressure, wash three times with alcohol, and dry to obtain the modified avermectin residue powder.

[0007] Preferably, in the step (1), the parameters of the drying treatment are: the drying temperature in the oven is 100 - 120 °C, the drying time is 2 - 8 h, and the sieving is through a 50 - 100 mesh sieve.

[0008] Preferably, in the step (1), the ozone input amount is 200 - 800 mL / min, and the ozone input time is 2 - 6 h.

[0009] Preferably, in the step (1), the alkali solution is a 0.1 - 1 mol / L sodium hydroxide solution, and the mass ratio of the avermectin residue powder to the sodium hydroxide solution is 1:10 - 30; the temperature of the alkali treatment is 60 - 90 °C, and the treatment time is 1 - 3 h.

[0010] Preferably, in the step (2), the organic solvent is at least one of toluene, dioxane, tetrahydrofuran, and dichloromethane.

[0011] Preferably, in the step (2), the mass ratio of the alkali-treated avermectin residue powder, tris(2-carboxyethyl)isocyanurate, and the organic solvent is 1:0.17 - 0.34:10 - 20.

[0012] Preferably, in the step (2), the catalyst is sulfonic acid, and the addition amount is 1% - 5% of the mass of tris(2-carboxyethyl)isocyanurate.

[0013] Preferably, the phosphate fertilizer is at least one of superphosphate, monoammonium phosphate, diammonium phosphate, potassium dihydrogen phosphate, and diammonium hydrogen phosphate.

[0014] Preferably, the potassium fertilizer is at least one of potassium sulfate, potassium nitrate, and potassium dihydrogen phosphate.

[0015] Preferably, the mass ratio of the modified avermectin residue powder, phosphate fertilizer, and potassium fertilizer is 1:0.16 - 0.32:0.7 - 2.1.

[0016] Preferably, the addition amount of the microbial complex bactericide is 0.8% - 3.6% of the modified avermectin residue.

[0017] Preferably, the microbial complex bactericide includes Bacillus subtilis, Bacillus licheniformis, and Bacillus megaterium with a mass ratio of 1:2.5 - 3.5:1.5 - 2.5, and the effective viable count ≥ 2×10 9 cfu / g.

[0018] Preferably, the particle size of the organic fertilizer is 2-8 mm, and after granulation, it is dried at 50-60 °C until the water content is less than 5%.

[0019] The beneficial effects of the present invention are as follows: 1. The present invention prepares an organic fertilizer added with avermectin residue. This organic fertilizer is obtained by modifying the existing avermectin residue, then compounding it with a microbial compound bacterium agent, and then mixing it with phosphate fertilizer and potassium fertilizer. The prepared organic fertilizer has achieved good results in improving the crop yield.

[0020] 2. The treatment of avermectin residue in the present invention is carried out by a method of compounding ozone and alkali first, aiming to kill avermectin in the residue, and then carrying out modification treatment. During this process, avermectin is further removed. After the residue is treated with alkali solution, functional groups such as hydroxyl groups exist on the surface. These characteristics endow the residue with excellent potential for matrix utilization. The present invention uses tris(2-carboxyethyl)isocyanurate for esterification modification treatment, introducing isocyanurate on the residue, making the nitrogen content of the residue richer. At the same time, as a carrier of the compound bacterium agent and fertilizer, the reduction, harmlessness and resource utilization of the residue are finally realized. Specific Embodiments

[0021] The technical solutions of the present invention are described below through specific specific examples. It should be understood that one or more method steps mentioned in the present invention do not exclude the existence of other method steps before and after the combined steps or the insertion of other method steps between these clearly mentioned steps; it should also be understood that these embodiments are only used to illustrate the present invention and not to limit the scope of the present invention. Moreover, unless otherwise specified, the numbers of each method step are only convenient tools for identifying each method step, rather than limiting the arrangement order of each method step or the scope of implementation of the present invention. The change or adjustment of their relative relationship, without substantial change in the technical content, should also be regarded as the scope of implementation of the present invention.

[0022] In order to better understand the above technical solutions, the exemplary embodiments of the present invention are described in more detail below. Although the exemplary embodiments of the present invention are shown, it should be understood that the present invention can be implemented in various forms and should not be limited by the embodiments described herein. On the contrary, these embodiments are provided to enable a more thorough understanding of the present invention and to be able to fully convey the scope of the present invention to those skilled in the art.

[0023] The present invention is further described below in conjunction with the following embodiments.

[0024] Example 1 An organic fertilizer added with avermectin residue, the preparation method comprising the following steps: Step (1): Dry the avermectin residue. The drying temperature is 110°C, and the drying time is 5 h. Grind it through an 80-mesh sieve, then place it in a 0.5 mol / L sodium hydroxide solution. First, introduce ozone for treatment. The ozone flow rate is 500 mL / min, and the ozone introduction time is 4 h. After removing the ozone, raise the temperature to 70°C and stir for 2 h. The mass ratio of the avermectin residue powder to the sodium hydroxide solution is 1:20. Then dry it again to obtain the alkali-treated avermectin residue powder. Step (2): Mix the alkali-treated avermectin residue powder with toluene. After stirring evenly, add tris(2-carboxyethyl)isocyanurate. The mass ratio of the alkali-treated avermectin residue powder, tris(2-carboxyethyl)isocyanurate, and toluene is 1:0.25:15. Dropwise add sulfonic acid as a catalyst, and the addition amount is 3% of the mass of tris(2-carboxyethyl)isocyanurate. Raise the temperature to 85°C and keep stirring for 3 h. After the reaction ends, remove the solvent under reduced pressure, wash it three times with alcohol, and dry it to obtain the modified avermectin residue powder. Step (3): First, mix the modified avermectin residue with a microbial complex agent. The addition amount of the microbial complex agent is 2.4% of the modified avermectin residue. The microbial complex agent includes Bacillus subtilis, Bacillus licheniformis, and Bacillus megaterium with a mass ratio of 1:3:2, and the effective viable count ≥ 2×10 9 cfu / g. Then compound it with superphosphate and potassium sulfate and granulate. The mass ratio of the modified avermectin residue powder, superphosphate, and potassium sulfate is 1:0.24:1.4. Dry it at 55°C until the water content is lower than 5% to prepare organic fertilizer granules with a particle size of 4 mm.

[0025] Example 2 An organic fertilizer added with avermectin residue, and the preparation method includes the following steps: Step (1): Dry the avermectin residue. The drying temperature is 100°C, and the drying time is 2 h. Grind it through a 50-mesh sieve, then place it in a 0.1 mol / L sodium hydroxide solution. First, introduce ozone for treatment. The ozone flow rate is 200 mL / min, and the ozone introduction time is 6 h. After removing the ozone, raise the temperature to 60°C and stir for 1 h. The mass ratio of the avermectin residue powder to the sodium hydroxide solution is 1:10. Then dry it again to obtain the alkali-treated avermectin residue powder. Step (2): Mix the alkali-treated avermectin residue powder with dioxane. After stirring evenly, add tris(2-carboxyethyl)isocyanurate. The mass ratio of the alkali-treated avermectin residue powder, tris(2-carboxyethyl)isocyanurate, and dioxane is 1:0.17:10. Dropwise add sulfonic acid as a catalyst, and the addition amount is 1% of the mass of tris(2-carboxyethyl)isocyanurate. Raise the temperature to 70°C and keep stirring for 2 h. After the reaction ends, remove the solvent under reduced pressure, wash it three times with alcohol, and dry it to obtain the modified avermectin residue powder. Step (3): First, mix the modified avermectin bacterial residue with a microbial complex agent. The addition amount of the microbial complex agent is 0.8% of the modified avermectin bacterial residue. The microbial complex agent includes Bacillus subtilis, Bacillus licheniformis, and Bacillus megaterium with a mass ratio of 1:2.5:1.5, and the effective viable count is ≥ 2×10 9 cfu / g. Then, compound it with monoammonium phosphate and potassium nitrate and granulate. The mass ratio of the modified avermectin bacterial residue powder, monoammonium phosphate, and potassium nitrate is 1:0.16:0.7. Dry it at 50°C until the water content is lower than 5% to prepare organic fertilizer granules with a particle size of 2 mm.

[0026] Example 3 An organic fertilizer added with avermectin bacterial residue, and the preparation method includes the following steps: Step (1): Dry the avermectin bacterial residue. The drying temperature is 120°C, and the drying time is 8 h. Grind it through a 100-mesh sieve, and then place it in a 1 mol / L sodium hydroxide solution. First, introduce ozone for treatment. The ozone introduction amount is 400 mL / min, and the ozone introduction time is 4 h. After removing the ozone, raise the temperature to 90°C and stir for 3 h. The mass ratio of the avermectin bacterial residue powder to the sodium hydroxide solution is 1:30. Then dry it again to obtain the alkali-treated avermectin bacterial residue powder; Step (2): Mix the alkali-treated avermectin bacterial residue powder with tetrahydrofuran, stir evenly, and then add tris(2-carboxyethyl)isocyanurate. The mass ratio of the alkali-treated avermectin bacterial residue powder, tris(2-carboxyethyl)isocyanurate, and tetrahydrofuran is 1:0.34:20. Dropwise add sulfonic acid as a catalyst, and the addition amount is 5% of the mass of tris(2-carboxyethyl)isocyanurate. Raise the temperature to 100°C, keep warm and stir for 4 h. After the reaction ends, remove the solvent under reduced pressure, wash it three times with alcohol, and dry it to obtain the modified avermectin bacterial residue powder; Step (3): First, mix the modified avermectin bacterial residue with a microbial complex agent. The addition amount of the microbial complex agent is 3.6% of the modified avermectin bacterial residue. The microbial complex agent includes Bacillus subtilis, Bacillus licheniformis, and Bacillus megaterium with a mass ratio of 1:3.5:2.5, and the effective viable count is ≥ 2×10 9 cfu / g. Then, compound it with diammonium phosphate and potassium dihydrogen phosphate and granulate. The mass ratio of the modified avermectin bacterial residue powder, diammonium phosphate, and potassium dihydrogen phosphate is 1:0.32:2.1. Dry it at 60°C until the water content is lower than 5% to prepare organic fertilizer granules with a particle size of 2 - 8 mm.

[0027] Example 4 An organic fertilizer added with avermectin bacterial residue, and the preparation method includes the following steps: Step (1): Dry the avermectin residue. The drying temperature is 110°C, and the drying time is 5 h. Grind it through a 60-mesh sieve, and then place it in a 0.2 mol / L sodium hydroxide solution. First, introduce ozone for treatment. The ozone flow rate is 300 mL / min, and the ozone introduction time is 5 h. After removing the ozone, raise the temperature to 80°C and stir for 2 h. The mass ratio of the avermectin residue powder to the sodium hydroxide solution is 1:15. Then dry it again to obtain the alkali-treated avermectin residue powder. Step (2): Mix the alkali-treated avermectin residue powder with dichloromethane. After fully stirring evenly, add tris(2-carboxyethyl)isocyanurate. The mass ratio of the alkali-treated avermectin residue powder, tris(2-carboxyethyl)isocyanurate, and dichloromethane is 1:0.22:15. Dropwise add sulfonic acid as a catalyst, and the addition amount is 2% of the mass of tris(2-carboxyethyl)isocyanurate. Raise the temperature to 90°C and keep stirring for 3 h. After the reaction ends, remove the solvent under reduced pressure, wash it with alcohol three times, and dry it to obtain the modified avermectin residue powder. Step (3): First, mix the modified avermectin residue with a microbial complex bactericide. The addition amount of the microbial complex bactericide is 1.8% of the modified avermectin residue. The microbial complex bactericide includes Bacillus subtilis, Bacillus licheniformis, and Bacillus megaterium with a mass ratio of 1:3:2.5, and the effective viable count ≥ 2×10 9 cfu / g. Then compound it with diammonium hydrogen phosphate and potassium dihydrogen phosphate and granulate it. The mass ratio of the modified avermectin residue powder, diammonium hydrogen phosphate, and potassium dihydrogen phosphate is 1:0.20:1.2. Dry it at 55°C until the water content is less than 5% to prepare organic fertilizer granules with a particle size of 6 mm.

[0028] Example 5 An organic fertilizer added with avermectin residue, and the preparation method includes the following steps: Step (1): Dry the avermectin residue. The drying temperature is 100°C, and the drying time is 3 h. Grind it through a 70-mesh sieve, and then place it in a 0.3 mol / L sodium hydroxide solution. First, introduce ozone for treatment. The ozone flow rate is 800 mL / min, and the ozone introduction time is 2 h. After removing the ozone, raise the temperature to 70°C and stir for 2 h. The mass ratio of the avermectin residue powder to the sodium hydroxide solution is 1:25. Then dry it again to obtain the alkali-treated avermectin residue powder. Step (2): Mix the alkali-treated avermectin residue powder and tetrahydrofuran, stir well, add tris(2-carboxyethyl)isocyanurate. The mass ratio of the alkali-treated avermectin residue powder, tris(2-carboxyethyl)isocyanurate, and tetrahydrofuran is 1:0.29:20. Dropwise add sulfonic acid as a catalyst, with the addition amount being 2% of the mass of tris(2-carboxyethyl)isocyanurate. Heat up to 100 °C, keep stirring for 3 h. After the reaction ends, remove the solvent under reduced pressure, wash three times with alcohol, and dry to obtain the modified avermectin residue powder. Step (3): First, mix the modified avermectin residue with a microbial complex bactericide. The addition amount of the microbial complex bactericide is 2.2% of the modified avermectin residue. The microbial complex bactericide includes Bacillus subtilis, Bacillus licheniformis, and Bacillus megaterium with a mass ratio of 1:3:2, and the effective viable count ≥ 2×10 9 cfu / g. Then compound it with superphosphate and potassium nitrate and granulate. The mass ratio of the modified avermectin residue powder, superphosphate, and potassium nitrate is 1:0.28:1.8. Dry at 50 °C until the water content is less than 5% to prepare organic fertilizer granules with a particle size of 4 mm.

[0029] Example 6 An organic fertilizer added with avermectin residue, and the preparation method includes the following steps: Step (1): Dry the avermectin residue, with the drying temperature being 110 °C and the drying time being 5 h. Grind it through an 80-mesh sieve, then place it in a 0.5 mol / L sodium hydroxide solution, first introduce ozone for treatment, with the ozone introduction amount being 500 mL / min and the ozone introduction time being 4 h. After removing the ozone, heat up to 70 °C and stir for 2 h. The mass ratio of the avermectin residue powder to the sodium hydroxide solution is 1:20. Then dry again to obtain the alkali-treated avermectin residue powder. Step (2): Mix the alkali-treated avermectin residue powder and toluene, stir well, add tris(2-carboxyethyl)isocyanurate. The mass ratio of the alkali-treated avermectin residue powder, tris(2-carboxyethyl)isocyanurate, and toluene is 1:0.25:15. Dropwise add sulfonic acid as a catalyst, with the addition amount being 3% of the mass of tris(2-carboxyethyl)isocyanurate. Heat up to 85 °C, keep stirring for 3 h. After the reaction ends, remove the solvent under reduced pressure, wash three times with alcohol, and dry to obtain the modified avermectin residue powder. Step (3): First, mix the modified avermectin residue with a microbial complex bactericide. The addition amount of the microbial complex bactericide is 1.1% of the modified avermectin residue. The microbial complex bactericide includes Bacillus subtilis, Bacillus licheniformis, and Bacillus megaterium with a mass ratio of 1:2.8:1.6, and the effective viable count ≥ 2×10 9cfu / g, and then granulated after being compounded with superphosphate and potassium sulfate. The mass ratio of the modified avermectin residue powder, superphosphate and potassium sulfate is 1:0.18:0.9, and dried at 55°C until the water content is less than 5% to obtain organic fertilizer granules with a particle size of 4 mm.

[0030] Example 7 An organic fertilizer added with avermectin residue, and the preparation method includes the following steps: Step (1), drying the avermectin residue, the drying temperature is 110°C, the drying time is 5 h, grinding through an 80-mesh sieve, and then placing it in a 0.5 mol / L sodium hydroxide solution. First, ozone is introduced for treatment, the ozone introduction amount is 500 mL / min, the ozone introduction time is 4 h. After removing the ozone, the temperature is raised to 70°C and stirred for 2 h. The mass ratio of the avermectin residue powder to the sodium hydroxide solution is 1:20, and then dried again to obtain the alkali-treated avermectin residue powder. Step (2), mixing the alkali-treated avermectin residue powder with toluene, fully stirring evenly, and then adding tris(2-carboxyethyl)isocyanurate. The mass ratio of the alkali-treated avermectin residue powder, tris(2-carboxyethyl)isocyanurate and toluene is 1:0.25:15. Sulfonic acid is added dropwise as a catalyst, and the addition amount is 3% of the mass of tris(2-carboxyethyl)isocyanurate. The temperature is raised to 85°C and kept warm and stirred for 3 h. After the reaction is completed, the solvent is removed under reduced pressure, washed three times with alcohol, and dried to obtain the modified avermectin residue powder. Step (3), first mixing the modified avermectin residue with a microbial complex agent. The addition amount of the microbial complex agent is 1.5% of the modified avermectin residue. The microbial complex agent includes Bacillus subtilis, Bacillus licheniformis and Bacillus megaterium with a mass ratio of 1:3.1:1.8, and the effective viable count ≥ 2×10 9 cfu / g, and then granulated after being compounded with superphosphate and potassium sulfate. The mass ratio of the modified avermectin residue powder, superphosphate and potassium sulfate is 1:0.21:1.1, and dried at 55°C until the water content is less than 5% to obtain organic fertilizer granules with a particle size of 4 mm.

[0031] Example 8 An organic fertilizer added with avermectin residue, and the preparation method includes the following steps: Step (1), drying the avermectin residue, the drying temperature is 110°C, the drying time is 5 h, grinding through an 80-mesh sieve, and then placing it in a 0.5 mol / L sodium hydroxide solution. First, ozone is introduced for treatment, the ozone introduction amount is 500 mL / min, the ozone introduction time is 4 h. After removing the ozone, the temperature is raised to 70°C and stirred for 2 h. The mass ratio of the avermectin residue powder to the sodium hydroxide solution is 1:20, and then dried again to obtain the alkali-treated avermectin residue powder. Step (2): Mix the alkali-treated avermectin residue powder with toluene. After stirring well, add tris(2-carboxyethyl)isocyanurate. The mass ratio of the alkali-treated avermectin residue powder, tris(2-carboxyethyl)isocyanurate, and toluene is 1:0.25:15. Dropwise add sulfonic acid as a catalyst, and the addition amount is 3% of the mass of tris(2-carboxyethyl)isocyanurate. Heat up to 85°C and keep stirring for 3 h. After the reaction ends, remove the solvent under reduced pressure, wash three times with alcohol, and dry to obtain the modified avermectin residue powder. Step (3): First, mix the modified avermectin residue with a microbial compound bactericide. The addition amount of the microbial compound bactericide is 1.8% of the modified avermectin residue. The microbial compound bactericide includes Bacillus subtilis, Bacillus licheniformis, and Bacillus megaterium with a mass ratio of 1:2.6:2.2, and the effective viable count is ≥2×10 9 cfu / g. Then, compound it with superphosphate and potassium sulfate and granulate. The mass ratio of the modified avermectin residue powder, superphosphate, and potassium sulfate is 1:0.24:1.3. Dry it at 55°C until the water content is lower than 5% to prepare organic fertilizer granules with a particle size of 4 mm.

[0032] Example 9 An organic fertilizer added with avermectin residue, and the preparation method includes the following steps: Step (1): Dry the avermectin residue. The drying temperature is 110°C and the drying time is 5 h. Grind it through an 80-mesh sieve, and then place it in a 0.5 mol / L sodium hydroxide solution. First, introduce ozone for treatment. The ozone introduction amount is 500 mL / min, and the ozone introduction time is 4 h. After removing the ozone, heat up to 70°C and stir for 2 h. The mass ratio of the avermectin residue powder to the sodium hydroxide solution is 1:20. Then dry it again to obtain the alkali-treated avermectin residue powder. Step (2): Mix the alkali-treated avermectin residue powder with toluene. After stirring well, add tris(2-carboxyethyl)isocyanurate. The mass ratio of the alkali-treated avermectin residue powder, tris(2-carboxyethyl)isocyanurate, and toluene is 1:0.25:15. Dropwise add sulfonic acid as a catalyst, and the addition amount is 3% of the mass of tris(2-carboxyethyl)isocyanurate. Heat up to 85°C and keep stirring for 3 h. After the reaction ends, remove the solvent under reduced pressure, wash three times with alcohol, and dry to obtain the modified avermectin residue powder. Step (3): First, mix the modified avermectin residue with a microbial compound bactericide. The addition amount of the microbial compound bactericide is 2.2% of the modified avermectin residue. The microbial compound bactericide includes Bacillus subtilis, Bacillus licheniformis, and Bacillus megaterium with a mass ratio of 1:2.9:1.6, and the effective viable count is ≥2×10 9cfu / g, and then compounded with superphosphate and potassium sulfate and granulated. The mass ratio of the modified avermectin residue powder, superphosphate and potassium sulfate is 1:0.26:1.5, and it is dried at 55°C until the water content is less than 5%, and organic fertilizer granules with a particle size of 4 mm are prepared.

[0033] Example 10 An organic fertilizer added with avermectin residue, and the preparation method includes the following steps: Step (1), drying the avermectin residue, the drying temperature is 110°C, the drying time is 5 h, grinding through an 80-mesh sieve, and then placing it in a 0.5 mol / L sodium hydroxide solution. First, ozone is introduced for treatment, the ozone introduction amount is 500 mL / min, the ozone introduction time is 4 h. After removing the ozone, the temperature is raised to 70°C and stirred for 2 h. The mass ratio of the avermectin residue powder to the sodium hydroxide solution is 1:20, and then it is dried again to obtain the alkali-treated avermectin residue powder; Step (2), mixing the alkali-treated avermectin residue powder with toluene, stirring evenly, adding tris(2-carboxyethyl)isocyanurate. The mass ratio of the alkali-treated avermectin residue powder, tris(2-carboxyethyl)isocyanurate and toluene is 1:0.25:15. Sulfonic acid is added dropwise as a catalyst, and the addition amount is 3% of the mass of tris(2-carboxyethyl)isocyanurate. The temperature is raised to 85°C and kept warm and stirred for 3 h. After the reaction is completed, the solvent is removed under reduced pressure, washed three times with alcohol, and dried to obtain the modified avermectin residue powder; Step (3), first mixing the modified avermectin residue with a microbial complex agent. The addition amount of the microbial complex agent is 3.1% of the modified avermectin residue. The microbial complex agent includes Bacillus subtilis, Bacillus licheniformis and Bacillus megaterium with a mass ratio of 1:3.2:2.1, and the effective viable count ≥ 2×10 9 cfu / g, and then compounded with superphosphate and potassium sulfate and granulated. The mass ratio of the modified avermectin residue powder, superphosphate and potassium sulfate is 1:0.3:2, and it is dried at 55°C until the water content is less than 5%, and organic fertilizer granules with a particle size of 4 mm are prepared.

[0034] Comparative Example 1 An organic fertilizer added with avermectin residue, which is different from Example 1 in that the alkali-treated avermectin residue powder in Example 1 is used to replace the modified avermectin residue, and the preparation process includes: First, mix the alkali-treated avermectin residue powder with a microbial complex agent. The addition amount of the microbial complex agent is 2.4% of the alkali-treated avermectin residue powder. The microbial complex agent includes Bacillus subtilis, Bacillus licheniformis and Bacillus megaterium with a mass ratio of 1:3:2, and the effective viable count ≥ 2×10 9cfu / g, and then granulated after being compounded with superphosphate and potassium sulfate. The mass ratio of alkali-treated avermectin residue powder, superphosphate and potassium sulfate is 1:0.24:1.4, dried at 55 °C until the water content is lower than 5%, and organic fertilizer granules with a particle size of 4 mm are prepared.

[0035] Comparative Example 2 An organic fertilizer added with avermectin residue, different from Example 1 in that the modified avermectin residue is a mixture of tris(2-carboxyethyl)isocyanurate and alkali-treated avermectin residue powder, tris(2-carboxyethyl)isocyanurate and alkali-treated avermectin residue powder are mixed according to 0.25:1, and then compounded with a microbial compound bactericide, compounded with superphosphate and potassium sulfate and granulated, and the process is the same as that in Example 1.

[0036] Experimental detection For the biological fertilizers of Example 1 and Comparative Examples 1-2, specific application tests were carried out in pepper cultivation. The location was a certain place in Yancheng, Jiangsu. The variety was Chaotianjiao No. 6. Sowing was carried out in March. Before sowing, deep plowing and sunning were carried out 2-3 times. The young pepper seedlings (3-4 true leaves) were planted in the land. The seedbed was 1 m wide and the bed soil was 10 cm thick. Fertilization started 7 days after normal watering. The fertilization amount was 100 g / m 2 , and harvested in July, and the corresponding indexes were measured. The results are shown in Table 1.

[0037]

[0038] Among them, the avermectin residue was provided by a pharmaceutical company in Jiangsu and transported through cold chain. It was stored at 4 °C before use. The avermectin content (%) = (the mass of avermectin in the avermectin residue organic fertilizer / the mass of avermectin in the avermectin residue before treatment) * 100%.

[0039] It can be seen from Table 1 that the avermectin content of the organic fertilizer using Example 1 of the present invention is lower, indicating that its treatment effect on the residue is better; in the cultivation of Chaotianjiao, the organic fertilizer of Example 1 has better comprehensive performance, whether it is plant height, fruit length, the number of fruits per plant and single fruit weight are better than those of the comparative examples, indicating that using the fertilizer of Example 1 of the present invention has a better fertilizer effect.

[0040] In the description of this specification, the descriptions referring to terms such as "one embodiment", "some embodiments", "examples", "specific examples", or "some examples", etc., mean that the specific features, structures, materials, or characteristics described in connection with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representations of the above terms should not be understood as necessarily referring to the same embodiment or example. Moreover, the specific features, structures, materials, or characteristics described can be combined in a suitable manner in any one or more embodiments or examples. In addition, those skilled in the art can combine and combine the different embodiments or examples described in this specification.

[0041] Although the embodiments of the present invention have been shown and described above, it can be understood that the above embodiments are exemplary and should not be construed as limiting the present invention. Those of ordinary skill in the art can make changes, modifications, substitutions, and variations to the above embodiments within the scope of the present invention.

Claims

1. An organic fertilizer added with avermectin bacterial residue, characterized in that, The organic fertilizer is obtained by mixing modified avermectin bacterial residue with a microbial compound bacterial agent, and then compounding with phosphate fertilizer and potassium fertilizer followed by granulation.

2. The organic fertilizer adding avermectin bacterial residue according to claim 1, characterized in that, The preparation method of the modified avermectin bacterial residue includes the following steps: Step (1), after drying the avermectin bacterial residue, grinding and sieving it, then placing it in an alkali solution, first introducing ozone for treatment, removing ozone and then heating and stirring, and then drying again to obtain alkali-treated avermectin bacterial residue powder; Step (2), mixing the alkali-treated avermectin bacterial residue powder with an organic solvent, stirring well, adding tris(2-carboxyethyl)isocyanurate, dropping a catalyst, heating to 70-100 °C, keeping warm and stirring for 2-4 h, after the reaction ends, removing the solvent under reduced pressure, washing three times with alcohol, and drying to obtain modified avermectin bacterial residue powder.

3. The organic fertilizer adding avermectin residue according to claim 2, wherein In the said step (1), the parameters of the drying treatment are: the drying temperature in the oven is 100-120 °C, and the drying time is 2-8 h; the sieving is through a 50-100 mesh sieve.

4. The organic fertilizer adding avermectin residue according to claim 2, characterized in that, In the said step (1), the alkali solution is a 0.1-1 mol / L sodium hydroxide solution; the ozone introduction amount is 200-800 mL / min, and the ozone introduction time is 2-6 h; the mass ratio of the avermectin bacterial residue powder to the sodium hydroxide solution is 1:10-30; the alkali treatment temperature is 60-90 °C, and the treatment time is 1-3 h.

5. The organic fertilizer adding avermectin bacterial residue according to claim 2, characterized in that, In the said step (2), the organic solvent is at least one of toluene, dioxane, tetrahydrofuran, and dichloromethane; the mass ratio of the alkali-treated avermectin bacterial residue powder, tris(2-carboxyethyl)isocyanurate, and the organic solvent is 1:0.17-0.34:10-20.

6. The organic fertilizer adding avermectin bacterial residue according to claim 2, characterized in that, In the said step (2), the catalyst is sulfonic acid, and the addition amount is 1%-5% of the mass of tris(2-carboxyethyl)isocyanurate.

7. An organic fertilizer adding avermectin bacterial residue according to claim 1, characterized in that, The phosphate fertilizer is at least one of superphosphate, monoammonium phosphate, diammonium phosphate, potassium dihydrogen phosphate, and diammonium hydrogen phosphate; the potassium fertilizer is at least one of potassium sulfate, potassium nitrate, and potassium dihydrogen phosphate.

8. An organic fertilizer adding avermectin residue as claimed in claim 1, wherein, The mass ratio of the modified avermectin bacterial residue powder, phosphate fertilizer, and potassium fertilizer is 1:0.16-0.32:0.7-2.

1.

9. An organic fertilizer adding avermectin bacteria residue according to claim 1, characterized in that The addition amount of the microbial complex bactericide is 0.8%-3.6% of the modified avermectin bacterial residue; the microbial complex bactericide comprises Bacillus subtilis, Bacillus licheniformis and Bacillus megaterium with a mass ratio of 1:2.5-3.5:1.5-2.5, and the effective viable count is ≥2×10 9 cfu / g.

10. The organic fertilizer adding avermectin bacterial residue according to claim 1, characterized in that The particle size of the organic fertilizer is 2-8 mm, and after granulation, it is dried at 50-60 °C until the water content is less than 5%.

Images