Poria cocos sexual strain

Abstract

The invention discloses a poria cocos sexual strain which is named as XJ-YX01, has the preservation number of CGMCCNO.41695 and the preservation date of December 13, 2024, and is preserved in the China General Microbiological Culture Collection Center (CGMCC). Compared with a strain obtained through traditional asexual propagation, the poria cocos sexual strain is higher in growth speed, compact and white in hypha, not prone to aging and higher in physiological activity.

Description

Technical Field

[0001] The present invention relates to the technical field of Wolfiporia cocos strain cultivation, and particularly to a sexual strain of Wolfiporia cocos. Background Art

[0002] Wolfiporia cocos is a fungus of the family Polyporaceae in the Basidiomycota. Its dried sclerotium is a traditional bulk Chinese medicinal material, and is known as "nine prescriptions out of ten contain Wolfiporia cocos", with a compatibility rate as high as 70% in common traditional Chinese medicine prescriptions, and has important food and medicinal values. It is also a best-selling medicinal material in domestic and foreign markets. Since 2018, its export volume has basically remained at about 4,000 t. According to statistics, there are only more than 40 strains of Wolfiporia cocos strain resources in the country, and only strains such as 5.78, Z1, and Xiangjing 28, which have excellent quality and are widely used, have relatively close genetic relationships. Genetic diversity analysis shows that their genetic backgrounds are narrow. However, the long-term asexual propagation of Wolfiporia cocos has caused the degeneration of Wolfiporia cocos strains to become increasingly serious, resulting in increasingly prominent problems such as increased pests and diseases in Wolfiporia cocos cultivation in some areas, decreased utilization rate of pine wood, unstable yields, and decreased quality of medicinal materials, severely restricting the development of the Wolfiporia cocos industry. There is an urgent need to carry out sexual breeding of Wolfiporia cocos to drive genetic recombination and promote the replacement of strains and the sustainable development of the industry. Summary of the Invention

[0003] Based on the technical problems existing in the background art, the present invention provides a sexual strain of Wolfiporia cocos, which has the advantages of fast growth rate, dense and white mycelium, not easy to age, and strong physiological activity.

[0004] A sexual strain of Wolfiporia cocos provided by the present invention is named XJ-YX01, with a preservation number of CGMCC NO. 41695, a preservation date of December 13, 2024, and is preserved in the China General Microbiological Culture Collection Center. The taxonomic name is Wolfiporia cocos.

[0005] Advantageous Technical Effects of the Present Invention

[0006] Compared with the strains obtained by traditional asexual reproduction, the sexual strain of Wolfiporia cocos cultivated by the present invention has a faster growth rate, dense and white mycelium, is not easy to age, and has stronger physiological activity. Brief Description of the Drawings

[0007] Figure 1 It is the structure of the mating experiment of basidiospores and mycelia proposed by the present invention; A: The mycelia cannot be paired. A1 is the front side of the culture medium, and A2 is the back side of the culture medium; B: The sexual hybrid mycelium XJ-YX01 produced by successful pairing of mycelia. B1 is the front side of the culture, and B2 is the back side of the culture;

[0008] Figure 2 It is the growth situation of different mycelia in the bagged medium for Wolfiporia cocos cultivation proposed by the present invention. Detailed Embodiments

[0009] The present invention will be further explained below in conjunction with specific embodiments.

[0010] Embodiment

[0011] Collect Poria cocos, select the central part of the sclerotium, isolate the mycelium under sterile conditions, and culture it in PDA medium. The culture conditions are 26°C, dark, and the culture time is 8 days. After covering the plate, place it in a 4°C refrigerator for standby, numbered XJ28.

[0012] Take the XJ28 mycelium, inoculate it into PDA medium with 2.0 g of wood chips added per 100 mL, at 26°C, light intensity of 1200 lx, and culture time of 37 days to obtain mature fruiting bodies.

[0013] Take the mature fruiting bodies of XJ28, obtain basidiospores by the Tween elution method, dilute them and inoculate them on PDA plate medium. The culture conditions are dark culture for 10 days at 26°C, then transfer them to the fruiting body medium, and screen the mycelium that has not produced fruiting bodies, which is the basidiospore mycelium.

[0014] Take 10 groups of basidiospore mycelia, numbered XJ28-01 to XJ28-10 respectively, pair the mycelia developed from different basidiospores in pairs for mating experiments, at 26°C, light intensity of 1200 lx, add 2.0 g of wood chips per 100 mL of PDA medium, and culture time of 37 days to obtain the sexual hybrid strain XJ-YX01 with mycelium fusion and capable of producing fruiting bodies.

[0015] Detect the growth characteristics and physiological activities of strain XJ-YX01, strain XJ28 (traditional asexual strain), and XJ28-01 (strain developed from basidiospores). The results are shown in Table 1.

[0016] Growth characteristics detection: Measure the mycelial growth rate with a vernier caliper, and take pictures to record the growth state and color of the mycelium, etc.

[0017] Laccase activity determination: Use laccase to decompose the substrate ABTS to produce ABTS free radicals. The absorption coefficient at 420 nm is much larger than that of the substrate ABTS. Measure the increase rate of ABTS free radicals and calculate the laccase activity. Use a kit purchased from Beijing Solarbio Science & Technology Co., Ltd. and detect according to the steps of the kit. The calculation formula is: Laccase activity = 61.7 × A ÷ W.

[0018] Table 1 Detection results of strain growth characteristics and physiological activities

[0019]

[0020] The detection results of the growth characteristics and physiological activities of different strains in the fungus bags are shown in Table 1. It was found that the starting day of growth of XJ-YX01 was 7 days, while the starting days of growth of XJ28 and XJ28-01 were both 9 days. In terms of growth rate, XJ-YX01 was also significantly faster than XJ28 and XJ28-01. After 18 days of inoculation, XJ-YX01 almost filled the fungus bag. After 33 days of inoculation, XJ-YX01 filled the fungus bag, and XJ28 and XJ28-01 filled the fungus bag in about 40 days.

[0021] From Figure 2 It can be seen that the mycelium of XJ-YX01 is dense and white; the mycelium of XJ28-01 is thin, and the mycelium is slightly yellowish-brown. The density of the mycelium of XJ28 is between that of XJ-YX01 and XJ28-01, and the mycelium is slightly yellowish-brown. Generally, the yellowing of the Poria cocos mycelium indicates that the mycelium begins to age and its growth vitality weakens.

[0022] Figure 1 It indicates the mating experiment of basidiospore mycelium. A is the mycelium that cannot be paired, A1 is the front side of the culture, and A2 is the back side of the culture; B is the XJ-YX01 sexual hybrid mycelium produced by successful mycelium pairing, B1 is the front side of the culture, and B2 is the back side of the culture.

[0023] Although the embodiments of the present application have been shown and described, for those of ordinary skill in the art, it can be understood that various changes, modifications, substitutions, and variations can be made to these embodiments without departing from the principles and spirit of the present application. The scope of the present application is defined by the appended claims and their equivalents, and all should be included within the protection scope of the present application.

Claims

1. A sexual strain of Wolfiporia cocos, characterized in that, Named XJ-YX01, deposit number CGMCC NO. 41695, deposited on December 13, 2024 at the China General Microbiological Culture Collection Center.

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