New tremella auramtialba strain JSJ-J2HD40-HD13 bred by space breeding technology and application thereof

Abstract

The invention relates to a new strain JSJ-J2HD40-HD13 of Naematia auramtiala, which is bred by a space breeding technology, and an application of the new strain JSJ-J2HD40-HD13, and belongs to the technical field of edible mushroom breeding. The new strain of the tremella aurantialba is preserved in the China General Microbiological Culture Collection Center with the preservation number of CGMCC No.41789, and the tremella aurantialba and a stereum hirsutum strain JSJ-G2YX001C which is preserved in the China General Microbiological Culture Collection Center with the preservation number of CGMCC No.41790 are cultured and bred under a dark culture condition to obtain a full-yellow tremella aurantialba sporocarp strain. The novel tremella aurantialba strain can omit color changing conditions, save production cost and improve production benefits, can be used as an excellent strain resource for genetic breeding of tremella aurantialba species, researches color-related gene information, and provides an important theoretical basis for gene positioning breeding.

Description

Technical Field

[0001] The invention belongs to the technical field of edible fungus breeding, and specifically relates to a new strain of golden fungus JSJ-J2HD40-HD13 bred by aerospace breeding technology and an application thereof. Background Art

[0002] Golden ear ( Naematelia aurantialba ) belongs to the phylum Basidiomycota, class Tremellomycetes, orders Tremellales, family Naemateliaceae, genus Naemateliaceae Naematelia The fruiting body of Auricularia auricula is composed of Auricularia auricula and Auricularia tricholoma ( Stereum hirsutum ) together to form a heterogeneous complex with a smooth taste and rich nutrition, rich in amino acids, dietary fiber, β-carotene and vitamin B2. The biological characteristics of golden ear itself are relatively special. It is composed of two species, so the entire breeding system is relatively complex and difficult. At present, most of the new strains of golden ear that have been publicly reported to be bred are systematically bred, that is, new varieties are bred through domestication and cultivation of wild resources. Systematic breeding of new varieties is one of the commonly used methods for variety breeding. Although this method is simple to operate and low in cost, it has a small genetic basis, a long breeding cycle and low efficiency. In addition, the golden ear strains currently cultivated artificially in the market have white primordia under dark culture conditions, and the primordia expand and grow to form fruiting bodies, which also remain white. Only after the light color change conditions are given during the growth process, the fruiting bodies will change color to yellow and then mature. Recently, a report on the use of space breeding technology to select new varieties of golden ear was published (CN119464086A), which revealed that a golden ear fruiting body selected and bred by the Kunming Institute of Botany, Chinese Academy of Sciences through space travel had a higher total flavonoids content than golden ear fruiting bodies that were not bred through space travel.

[0003] Due to its complex biological characteristics, the golden ear mushroom industry has developed later than other major edible fungi, and the development of strain resources is relatively small. In order to ensure the sustainable and healthy development of the golden ear mushroom industry, it is necessary to have more strains with excellent traits for subsequent gene-directed breeding and other work. Therefore, it is extremely important to develop new varieties of golden ear mushrooms through different breeding methods and screen strains with excellent traits.

[0004] Related references on golden ear can be found in: [1] Yunnan Junshijie Biotechnology Co., Ltd. A strain of Auricularia auricula JSJ-J2X1001W and its application and molecular marker identification method: CN118272238A.2024-07-02. [2] Yunnan Junshijie Biotechnology Co., Ltd. A strain of Auricularia auricula JSJ-J2F1001C and its application and molecular marker identification method: CN118207101A.2024-06-18. [3] Yunnan Junshijie Biotechnology Co., Ltd. A Tremella aurantialba strain suitable for industrial cultivation: CN112592835A. April 2, 2021. [4] Kunming Edible Fungi Research Institute, All-China Federation of Supply and Marketing Cooperatives. Two new Tremella aurantialba strains and their SSR molecular marker identification methods: CN115261236A. November 1, 2022. [5] Kunming Edible Fungi Research Institute, Yunnan Supply and Marketing Cooperatives Scientific Research Institute. A new Tremella aurantialba strain, Junjin'er No. 1 strain, and its cultivation method: CN111990164A[P]. November 27, 2020. [6] Kunming Institute of Botany, Chinese Academy of Sciences. A space-borne Tremella cinnabarina strain, Hang 8, and its application: CN119464086A. February 18, 2025. [7] Cao Yao, Shen Zhenhui, Yang Linlei, etc. Determination of the polarity of Tremella aurantialba using single spore hybridization technology [J]. Acta Edulis Fungi. 2024, 31(01): 38-44. SUMMARY OF THE INVENTION The object of the present invention is to provide a new strain of Tremella aurantialba, JSJ-J2HD40-HD13, selected by space breeding technology and its application.

[0005] The technical solution adopted by the present invention is as follows: A new strain of Tremella aurantialba ( Naematelia aurantialba ) JSJ-J2HD40-HD13, which is preserved in the China General Microbiological Culture Collection Center, with the preservation number CGMCC No. 41789.

[0006] The application of the new strain of Tremella aurantialba ( Naematelia aurantialba ) JSJ-J2HD40-HD13 selected by space breeding technology of the present invention is to use the new strain JSJ-J2HD40-HD13 to cultivate and produce Tremella aurantialba seeds with the strain JSJ-G2YX001C of Stereum hirsutum ( stereum hirsutum ), and the fruiting bodies of the Tremella aurantialba seeds are yellow from the primordium to the mature stage under dark culture conditions; the Stereum hirsutum strain JSJ-G2YX001C is preserved in the China General Microbiological Culture Collection Center, with the preservation number CGMCC No. 41790.

[0007] The strain provided by the present invention is yellow from the primordium stage to the mature stage, and does not require additional color conversion conditions to turn yellow. The color conversion conditions can be omitted, saving production costs and improving production efficiency. Moreover, it can be used as an excellent strain resource for the genetic breeding of Tremella aurantialba, study the gene information related to color, and provide an important theoretical basis for gene mapping breeding. BRIEF DESCRIPTION OF THE DRAWINGS

[0008] Figure 1 It is a photograph of the biological characteristics of the Tremella aurantialba strain JSJ-J2HD40-HD13; Figure 2 It is a photograph of the biological characteristics of the Stereum hirsutum strain JSJ-G2YX001C; Figure 3 It is the fruiting body of the fungus obtained by culturing the two compatible monokaryotic spore strains HD40 and HD13 of Tremella aurantialba carried and Stereum hirsutum JSJ-G2YX001C; Figure 4 It is the fruiting body of the fungus obtained by culturing the two compatible monokaryotic spore strains HD40 and HD13 of Tremella aurantialba without carrying and Stereum hirsutum JSJ-G2YX001C.

[0009] Figure 5 It is the fruiting body of the fungus obtained by culturing the Tremella aurantialba strain JSJ-J2HD40-HD13 and Stereum hirsutum JSJ-G2YX001C; DETAILED DESCRIPTION OF THE EMBODIMENTS

[0010] The content of the present invention will be further elaborated below in conjunction with specific embodiments.

[0011] A new strain of Tremella aurantialba JSJ-J2HD40-HD13 obtained by space breeding technology, the Tremella aurantialba strain JSJ-J2HD40-HD13 is deposited in the China General Microbiological Culture Collection Center (CGMCC), the deposit number is: CGMCC No. 41789, the deposit date is February 19, 2025, and the taxonomic name is Tremella aurantialba Naematelia aurantialba , which belongs to the family Naemateliaceae, genus Naematelia Naematelia ). It is an asexual spore, the colony is smooth and milky white. See Figure 1 .

[0012] The said strains were obtained by smearing 44 Tremella aurantialba materials (see Table 1) of Yunnan Junsijie Biotechnology Co., Ltd. on weighing paper. After air-drying the weighing paper, it was put into a 0.2 mL PCR tube and carried out space mutagenesis on Shenzhou-15 on November 29, 2022, among which there were 10 monokaryotic strains and 34 heterokaryotic strains. The control materials of the above 44 Tremella aurantialba strains were stored in a 4°C refrigerator. All the carried materials were returned on July 5, 2023, and the carried and control materials were simultaneously activated under the culture conditions of PDA medium, 20°C, and darkness. Among them, a total of 64 carried and control materials were successfully activated (see Table 1). All the successfully activated control and carried materials were cultured and compared with the Stereum hirsutum strain JSJ-G2YX001C to observe whether the fruiting body traits of each control and carried material changed during cultivation.

[0013] Table 1 List of Materials of Tremella aurantialba Carried by Shenzhou-15 of Yunnan Junsijie Biotechnology Co., Ltd. and Activation Status

[0014]

[0015]

[0016]

[0017] In the table, "√" indicates successful activation; "×" indicates failed activation.

[0018] The Stereum hirsutum strain JSJ-G2YX001C was isolated from the fruiting body of Stereum hirsutum collected in Yuxi City, Yunnan Province, and is preserved in the China General Microbiological Culture Collection Center (CGMCC), with the preservation number: CGMCC No. 41790, and the preservation date is February 19, 2025, and the taxonomic name is Stereum hirsutum stereum hirsutum , which belongs to the family Stereaceae ( Stereum hirsutum ), genus Stereum ( Stereum ). It is in the mycelial state, with a circular and white colony. See Figure 2 .

[0019] The steps of the culture comparison experiment between the control and carried materials and Stereum hirsutum JSJ-G2YX001C are as follows: Prepare the liquid strain of Stereum hirsutum. Culture medium: 200 g of potatoes (boiled juice), 20 g of glucose, 3 g of potassium dihydrogen phosphate, 1.5 g of magnesium sulfate, 1000 mL of water. After autoclaving and cooling, inoculate 20 mycelial agar blocks of strain JSJ-G2YX001C with a size of 5 mm×5 mm×5 mm, and place them on a magnetic stirrer at 21 °C with a rotation speed of 180 for 5 days; All successfully activated materials contain Tremella aurantialba monokaryon spores and asexual spore materials. For the monokaryon spore materials, two compatible strains need to be cultured with Stereum hirsutum JSJ-G2YX001C simultaneously. In PDA medium, respectively pick 0.5 g of two compatible monokaryon spores that have been successfully activated and cultured for 10 d, mix them with 4 mL of the liquid strain of Stereum hirsutum, and then inoculate them into the culture substrate (formula: 30% cottonseed hull, 56% wood chips, 12% wheat bran, 1% potassium dihydrogen phosphate, 1% calcium sulfate, water content 55% - 65%, pH 6 - 6.5; 130 g of culture substrate is filled in a 240 mL original seed bottle), and culture at 18 °C, relative humidity 50%, CO2 mass concentration ≤ 800 mg·L -1 , observe the occurrence of primordia under dark conditions during the culture period, and transfer the primordia to a new original seed bottle for continuous culture; for the asexual spore materials, directly pick 0.5 g of the spores after the culture ends in PDA medium, mix them with 4 mL of the liquid strain of Stereum hirsutum, and then inoculate them into the culture substrate and culture according to the above conditions; all the materials carried by spacecraft and the control materials are cultured and cultivated for comparative experiments according to the above method; A total of 64 strains of all control and carried materials were cultured and cultivated with Stereum hirsutum, and 26 fruiting bodies were obtained. Among them, from the materials carried by spacecraft, Tremella aurantialba fruiting bodies obtained by culturing two compatible Tremella aurantialba monokaryon spore strains D40 and D13 with Stereum hirsutum JSJ-G2YX001C were yellow from the primordium growth to the mature fruiting body under dark culture conditions (see Figure 3 ); while the Tremella aurantialba fruiting bodies obtained by culturing the control materials D40 and D13 without being carried by spacecraft with Stereum hirsutum JSJ-G2YX001C were white from the primordium growth to the mature fruiting body under dark conditions (see Figure 4 ), that is, Tremella aurantialba fruiting bodies with a yellow color throughout the growth cycle were obtained by culturing two compatible monokaryon spore strains D40 and D13 carried by spacecraft with Stereum hirsutum JSJ-G2YX001C.

[0020] Extract and isolate asexual spores from the yellow Tremella aurantialba fruiting bodies obtained by culturing two compatible monokaryon spore strains D40 and D13 with Stereum hirsutum JSJ-G2YX001C, and then culture and verify the asexual spores with Stereum hirsutum JSJ-G2YX001C. The relevant extraction, separation, and culture steps are as follows: 1) Harvest the obtained yellow Tremella aurantialba fruit bodies and clean them thoroughly. Wipe the mushroom bodies with 75% alcohol, then rinse with sterile water and place them in a laminar flow hood to dry. 2) Take 10 tissue blocks of the fruit bodies and place them in a 250 mL liquid medium (the liquid medium is: 200 g of potato juice filtered after boiling, 20 g of glucose, 1000 mL of water); culture under the conditions of a rotation speed of 150 r / min and a temperature of 23 °C for 4 - 5 days; after the culture is completed, transfer 0.5 mL to a PDA medium, culture for 4 - 5 days, then separate the asexual spores in the medium to a new PDA medium and continue to culture for 4 - 5 days. Transfer the asexual spores to a new PDA medium again. After ITS identification, it is identified as Tremella aurantialba, and the asexual spores of Tremella aurantialba are obtained, named JSJ - J2HD40 - HD13. 3) Pick 0.5 g of the asexual spores of the JSJ - J2HD40 - HD13 strain and mix them with 4 mL of the liquid spawn of Stereum hirsutum JSJ - G2YX001C, and then inoculate them into the culture substrate (formula: 30% cottonseed hulls, 56% sawdust, 12% wheat bran, 1% potassium dihydrogen phosphate, 1% calcium sulfate, water content 55% - 65%, pH 6 - 6.5; 150 g of the culture substrate is filled in a 240 mL original seed bottle), and culture at 18 °C, relative humidity 50%, CO2 mass concentration ≤ 800 mg·L -1 , and observe the occurrence of primordia under dark conditions. During this period, transfer the primordia to a new original seed bottle for continuous culture.

[0021] 4) After 40 days of transfer culture through the occurrence of primordia, the asexual spores of the JSJ - J2HD40 - HD13 strain and the Stereum hirsutum JSJ - G2YX001C strain also obtained yellow Tremella aurantialba fruit bodies under dark culture conditions, as shown in Figure 5 .

[0022] The growth time of the yellow Tremella aurantialba fruit bodies obtained by culturing the JSJ - J2HD40 - HD13 strain is shortened by 5 days compared with the control Tremella aurantialba fruit bodies. It is used to prepare the spawn fruit bodies, and the spawn fruit bodies can be used in about 40 days, while the control spawn fruit bodies need about 45 days to be used for cultivation. The crude polysaccharide nutritional components of the two were detected, indicating that the crude polysaccharide content of the yellow Tremella aurantialba fruit bodies obtained by spaceflight is significantly increased compared with the control Tremella aurantialba fruit bodies (Table 2).

[0023] Table 2 Record table of relevant indicators of spawn fruit bodies cultured with and without spaceflight materials

Claims

1. Tremella aurantialba strain JSJ-J2HD40-HD13 selected by space breeding technology, characterized in that, Naematelia aurantialba The strain is deposited in the China General Microbiological Culture Collection Center with the deposit number of CGMCC No. 41789.

2. Use of the new strain JSJ-J2HD40-HD13 of Tremella aurantialba selected by space breeding technology as claimed in claim 1, characterized in that, Naematelia aurantialba The new strain JSJ-J2HD40-HD13 is used to cultivate Tremella aurantialba spawn with the strain of Stereum hirsutum ( stereum hirsutum ), and the spawn of Tremella aurantialba is a spawn fruiting body that is yellow from primordium to maturity under dark culture conditions; the strain of Stereum hirsutum JSJ-G2YX001C is deposited in the General Microbiology Center of the China Committee for Culture Collection of Microorganisms, and the deposit number is CGMCC No. 41790. ​

Images