Automatic biological sample refrigeration house based on inverse Brayton cycle refrigeration

Abstract

The automatic biological sample refrigeration house comprises a sample library arranged in a heat preservation house body, the heat preservation house body is divided into a sample storage room and an equipment room by a heat preservation partition plate, and the sample library is arranged in the sample storage room; the sample storage room provides a low-temperature environment for the sample library and is a transition storage room for sample transmission; a sample transfer window is arranged on the outer side wall of the sample storage room, and a sample transfer mechanism for transferring samples is further arranged in the sample storage room; a reverse Brayton cycle refrigeration device and a refrigeration and dehumidification device are arranged in the device room, the sample library is connected with the reverse Brayton cycle refrigeration device through an air inlet pipeline and an air return pipeline, and the refrigeration and dehumidification device is connected with the sample storage room through a pipeline; the device has the advantages of being good in sample freezing storage effect, small in influence of environment temperature on samples when the samples are stored and taken, low in energy consumption, high in storage density, large in capacity and the like.

Description

Technical Field

[0001] The present invention relates to the technical field of biological sample cold storage, and specifically to an automated biological sample cold storage based on reverse Brayton cycle refrigeration. Background Art

[0002] Biological sample banks are important basic facilities in the current pharmaceutical and biological research fields and application fields. Through low-temperature or cryogenic preservation, blood products, vaccines, stem cells, and vaccines can maintain their activity for a long time. The usage method is to store the samples in cryogenic tubes and then store the cryogenic tubes in a low-temperature biological sample storage device for long-term storage.

[0003] Patent Publication No. CN212299609U discloses a full-automatic sample refrigeration system, which specifically discloses that a refrigeration system is installed on a cold storage body. Inside the cold storage body, two shelves and a sample transfer manipulator are symmetrically arranged. The sample transfer manipulator is located between the two shelves and can move along a direction parallel to the shelves. The working area of the sample transfer manipulator covers the storage area of the shelves. However, there are problems such as a poor cryogenic storage environment in the cold storage, a large impact of the ambient temperature on the samples when accessing the samples, easy damage to the samples, and poor storage effects of biological samples. Summary of the Invention

[0004] The technical problem to be solved by the present invention is to overcome the existing defects and provide an automated biological sample cold storage based on reverse Brayton cycle refrigeration. The biological sample cold storage has a cryogenic and stable freezing storage environment, good sample freezing storage effects, a small impact of the ambient temperature on the samples when accessing the samples, a split type between the storage part and the refrigeration unit, low energy consumption, high storage density, and a large capacity, and can effectively solve the problems in the background art.

[0005] To achieve the above object, the present invention provides the following technical solution: An automated biological sample cold storage based on reverse Brayton cycle refrigeration, including a sample bank provided inside a heat-insulating cold storage body. A heat-insulating partition is provided inside the heat-insulating cold storage body, and the heat-insulating cold storage body is separated by the heat-insulating partition into a sample storage room and an equipment room. The sample bank is arranged in the sample storage room. The sample bank is used for freezing and storing samples. The sample storage room provides a low-temperature environment for the sample bank and is a transition storage room for sample transfer. A sample transfer window is provided on the outer side wall of the sample storage room at the sample storage room. A sample transfer mechanism for transferring samples between the sample transfer window and the sample bank is also provided inside the sample storage room. A reverse Brayton cycle refrigeration device and a refrigeration and dehumidification device are provided inside the equipment room. The sample bank is connected with an air inlet pipe and an air return pipe, and the air inlet pipe and the air return pipe penetrate through the heat-insulating partition and are respectively connected with the air supply pipe orifice and the air return pipe orifice of the reverse Brayton cycle refrigeration device. The refrigeration and dehumidification device is connected with the sample storage room through a pipe penetrating through the heat-insulating partition. A temperature and humidity sensor is provided inside the sample storage room, and a temperature sensor is provided inside the sample bank. The reverse Brayton cycle refrigeration equipment provides cooling capacity for the sample library through the air inlet duct and recovers the cooling capacity through the air return duct. The refrigeration and dehumidification equipment provides cooling capacity to the sample storage room, and the internal temperature of the sample storage room is controlled at -25°C through the refrigeration and dehumidification equipment. At the same time, the internal humidity of the sample storage room is kept below the dew point under the control of the refrigeration and dehumidification equipment, preventing frost formation on the surface of the sample storage container from affecting the barcode scanning and reading, and preventing condensation and icing on the outer wall of the sample library. Moreover, the internal temperature of the sample library is lower than the internal temperature of the sample storage room.

[0006] Furthermore, the sample library includes a sample storage repository body, which includes an upper and a lower distributed thermal insulation top plate and a thermal insulation bottom plate. Thermal insulation side plates are provided between the two side ends of the thermal insulation top plate and the thermal insulation bottom plate. An air inlet and an air outlet are provided on one of the thermal insulation side plates, and the air inlet duct and the air return duct are respectively connected to the air inlet and the air outlet. A thermal insulation back plate is provided between the backs of the thermal insulation top plate and the thermal insulation bottom plate; a storage shelf is provided inside the sample storage repository body.

[0007] Furthermore, the storage shelf includes a support frame, and a plurality of sample tray drawers that can slide back and forth along the sample storage repository body are provided inside the support frame. The push-pull end plate on the front side of the sample tray drawer is a pull-out thermal insulation door.

[0008] Furthermore, the thermal insulation top plate, the thermal insulation bottom plate, the thermal insulation side plates and the thermal insulation back plate are provided with polyurethane thermal insulation layers.

[0009] Furthermore, one end of both the air inlet duct and the air return duct horizontally penetrates into the sample storage repository body, and the lengths of the air inlet duct and the air return duct penetrating into the sample storage repository body are adapted to the length of the internal space of the sample storage repository body. A plurality of air holes are evenly provided on the pipe walls of the air inlet duct and the air return duct penetrating into the sample storage repository body.

[0010] Furthermore, a storage room maintenance door and a central control screen are also provided on the outer side wall of the thermal insulation repository body at the sample storage room, and an equipment room maintenance door is provided on the outer wall of the thermal insulation repository body at the equipment room.

[0011] Furthermore, an outer thermal insulation door is provided on the outer side wall of the thermal insulation repository body at the position corresponding to the sample transfer window, and an inner thermal insulation door is provided on the inner side wall of the thermal insulation repository body at the position corresponding to the sample transfer window.

[0012] Compared with the prior art, the beneficial effects of the present invention are as follows: The automated biological sample cold storage based on reverse Brayton cycle refrigeration divides the internal space of the heat-insulating storage body into a sample storage room and an equipment room through heat-insulating partition boards, avoiding the heat generated by the equipment in the equipment room from affecting the low-temperature storage environment of the samples. The storage part and the refrigeration unit are separated, with low energy consumption. Moreover, the sample library is arranged in the sample storage room, preventing the samples from being damaged due to exposure to the normal-temperature environment during the access process, and the environmental temperature has little impact on the samples. The sample storage room provides low-temperature protection for the freezing environment of the sample library. At the same time, the internal humidity of the sample storage room is kept below the dew point under the control of the refrigeration and dehumidification equipment, preventing frost formation on the surface of the sample storage container from affecting the barcode reading and preventing condensation and icing on the outer wall surface of the sample library. Utilizing the characteristics of large refrigeration capacity and stable refrigeration of the reverse Brayton cycle refrigeration equipment, the sample library has a deep-low-temperature and stable freezing storage environment. The biological sample cold storage is an integrated large-capacity structure with a high storage density. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] Figure 1 is a schematic structural diagram of the present invention; Figure 2 is a top view of the internal structure of the heat-insulating storage body of the present invention; Figure 3 is a side sectional view of the heat-insulating storage body of the present invention; Figure 4 is a schematic structural diagram of the sample library of the present invention; Figure 5 is a schematic structural diagram of the local structure of the storage shelf of the present invention; Figure 6 is a partially enlarged view of the storage shelf of the present invention; Figure 7 is a schematic connection structure diagram of the reverse Brayton cycle refrigeration equipment and the sample library of the present invention.

[0014] In the figure: 1, heat-insulating storage body; 101, sample storage room; 102, equipment room; 103, heat-insulating partition board; 104, maintenance door of the storage room; 105, sample transfer window; 1051, outer heat-insulating door; 1052, inner heat-insulating door; 106, maintenance door of the equipment room; 107, central control screen; 2, sample library; 21, sample storage library body; 211, heat-insulating top plate; 212, heat-insulating side plate; 213, heat-insulating bottom plate; 214, heat-insulating back plate; 215, air inlet; 216, air outlet; 217, polyurethane heat-insulating layer; 22, storage shelf; 221, support frame; 222, sample tray drawer; 223, pull-out heat-insulating door; 3, sample transmission mechanism; 4, reverse Brayton cycle refrigeration equipment; 41, air inlet pipe; 42, return air pipe; 43, air hole; 5, refrigeration and dehumidification equipment; 6, temperature and humidity sensor; 7, temperature sensor. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0015] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention. Embodiment

[0016] Please refer to Figure 1-7 , the present invention provides a technical solution: an automated biological sample cold storage based on reverse Brayton cycle refrigeration, including a sample storage 2 provided in a heat-insulating storage body 1. The heat-insulating storage body 1 is provided with a heat-insulating partition 103, and the heat-insulating storage body 1 is separated by the heat-insulating partition 103 into a sample storage room 101 and an equipment room 102. The sample storage 2 is arranged in the sample storage room 101. The sample storage 2 is used for freezing and storing samples. The sample storage room 101 provides a low-temperature environment for the sample storage 2 and is a transition storage room for sample transfer; a sample transfer window 105 is provided on the outer side wall of the sample storage room 101 at the position of the sample storage room 101. An outer heat-insulating door 1051 is provided on the outer side wall of the heat-insulating storage body 1 at the position corresponding to the sample transfer window 105. An inner heat-insulating door 1052 is provided on the inner side wall of the heat-insulating storage body 1 at the position corresponding to the sample transfer window 105; a sample transfer mechanism 3 for transferring samples between the sample transfer window 105 and the sample storage 2 is further provided in the sample storage room 101; a reverse Brayton cycle refrigeration device 4 and a refrigeration and dehumidification device 5 are provided in the equipment room 102. An air supply duct opening and a return air duct opening of the reverse Brayton cycle refrigeration device 4 are respectively connected with an air inlet duct 41 and a return air duct 42. The reverse Brayton cycle refrigeration device 4 is connected to the sample storage 2 through the air inlet duct 41 and the return air duct 42 penetrating through the heat-insulating partition 103. The refrigeration and dehumidification device 5 is connected to the sample storage room 101 through a pipeline penetrating through the heat-insulating partition 103; a temperature and humidity sensor 6 is provided in the sample storage room 101, and a temperature sensor 7 is provided in the sample storage 2; The sample library 2 includes a sample storage repository body 21. The sample storage repository body 21 includes a heat-insulating top plate 211 and a heat-insulating bottom plate 213 that are distributed vertically. Heat-insulating side plates 212 are provided between the two side ends of the heat-insulating top plate 211 and the heat-insulating bottom plate 213. An air inlet 215 and an air outlet 216 are formed on one of the heat-insulating side plates 212. The air inlet duct 41 and the return air duct 42 are respectively connected to the air inlet 215 and the air outlet 216. A heat-insulating back plate 214 is provided between the backs of the heat-insulating top plate 211 and the heat-insulating bottom plate 213. A storage shelf 22 is provided inside the sample storage repository body 21. The storage shelf 22 includes a support frame 221. A plurality of sample tray drawers 222 that can slide back and forth along the sample storage repository body 21 are provided inside the support frame 221. The push-pull end plate on the front side of the sample tray drawer 222 is a pull-out heat-insulating door 223. The reverse Brayton cycle refrigeration device 4, the refrigeration and dehumidification device 5, and the sample transfer mechanism 3 described in this embodiment are all prior arts in the field, and the structures and working principles thereof will not be described in detail in this embodiment; Working principle: The reverse Brayton cycle refrigeration device 4 provides cold energy for the sample library 2 through the air inlet duct 41, and recovers the cold energy through the return air duct 42. The refrigeration and dehumidification device 5 provides cold energy to the sample storage room 101, and controls the internal temperature of the sample storage room 101 to -25°C through the refrigeration and dehumidification device 5. At the same time, the internal humidity of the sample storage room 101 is kept below the dew point under the control of the refrigeration and dehumidification device 5, preventing frost formation on the surface of the sample storage container from affecting the barcode scanning and reading, and preventing condensation and icing on the outer wall surface of the sample library 2. And the internal temperature of the sample library 2 is lower than the internal temperature of the sample storage room 101; When storing samples, first open the outer heat-insulating door 1051 and close the inner heat-insulating door 1052, place the sample storage container in the sample transfer window 105, then close the outer heat-insulating door 1051 and open the inner heat-insulating door 1052. The sample transfer mechanism 3 clamps the sample storage container at the sample transfer window 105, and scans and selects the sample freezing tubes in the sample storage container through the sample transfer mechanism 3. Finally, the sample transfer mechanism 3 places the sample freezing tubes in the corresponding sample tray drawers 222. When selecting samples, the sample transfer mechanism 3 takes out the sample freezing tubes at the specified positions, places the sample freezing tubes in the sample storage container, and transfers the sample storage container to the sample transfer window 105, thus completing the work of taking out the samples; When the sample library 2 requires -80°C, the reverse Brayton cycle refrigeration device 4 controls the main engine to operate at a certain speed according to the temperature inside the sample library 2. When the required temperature becomes lower, the speed is increased; when the required temperature becomes higher, the speed is decreased. When a relatively large number of samples are placed, the temperature sensor 7 senses that the temperature increases, and the speed of the reverse Brayton refrigeration system main engine increases, and at the same time, the reference speed of -80°C is increased. When a small number of samples are placed, the temperature sensor 7 senses that the temperature decreases, and the speed of the reverse Brayton refrigeration system main engine decreases, and at the same time, the reference speed of -80°C is decreased.

[0017] Further, the heat-insulating top plate 211, the heat-insulating bottom plate 213, the heat-insulating side plates 212 and the heat-insulating back plate 214 are provided with a polyurethane heat-insulating layer 217; the heat-insulating performance of the sample library 2 is increased by the polyurethane heat-insulating layer 217.

[0018] Further, a storage room maintenance door 104 and a central control screen 107 are further provided on the outer wall of the heat-insulating library body 1 at the sample storage room 101, and an equipment room maintenance door 106 is provided on the outer wall of the heat-insulating library body 1 at the equipment room 102; it is convenient to repair the internal equipment of the sample storage room 101 and the sample library 2 through the storage room maintenance door 104, and it is convenient to repair the equipment in the equipment room 102 through the equipment room maintenance door 106.

[0019] Further, one end of each of the air inlet duct 41 and the air return duct 42 horizontally penetrates into the sample storage library body 21, and the lengths of the air inlet duct 41 and the air return duct 42 penetrating into the sample storage library body 21 are adapted to the length of the internal space of the sample storage library body 21. A plurality of air holes 43 are evenly formed on the pipe walls of the air inlet duct 41 and the air return duct 42 penetrating into the sample storage library body 21. The internal temperature distribution of the sample library 2 is made more uniform through the plurality of air holes 43 provided on the air inlet duct 41 and the air return duct 42, and the freezing storage effect of biological samples is further improved.

[0020] The automated biological sample cold storage based on reverse Brayton cycle refrigeration disclosed in this embodiment divides the internal space of the heat-insulating library body 1 into a sample storage room 101 and an equipment room 102 through a heat-insulating partition 103, avoiding the heat generated by the equipment in the equipment room 102 from affecting the low-temperature storage environment of the samples. The storage part and the refrigeration unit are split-type, with low energy consumption. Moreover, the sample library 2 is arranged in the sample storage room 101, avoiding damage to the samples caused by exposure to the normal temperature environment during the access process of the samples, and the environmental temperature has little influence on the samples; and the sample storage room 101 provides low-temperature protection for the freezing environment of the sample library 2. At the same time, the humidity inside the sample storage room 101 is kept below the dew point under the control of the refrigeration and dehumidification device 5, preventing frost formation on the surface of the sample freezing container from affecting the code scanning and reading and preventing condensation and icing on the outer wall surface of the sample library 2; taking advantage of the characteristics of large refrigeration capacity and stable deep-low-temperature refrigeration of the reverse Brayton cycle refrigeration device 4, the sample library 2 has a deep-low-temperature (-80 °C) and stable freezing storage environment; the biological sample cold storage is an integrated large-capacity structure with a high storage density.

[0021] Although the embodiments of the present invention have been shown and described, it will be understood by those of ordinary skill in the art that various changes, modifications, substitutions and variations can be made therein without departing from the principles and spirit of the present invention, and the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. An automated biological sample cold storage based on reverse Brayton cycle refrigeration, comprising a sample storage provided in a heat-insulated storage body, characterized in that: The heat-insulated storage body is provided with a heat-insulated partition board, and the heat-insulated storage body is separated into a sample storage room and an equipment room by the heat-insulated partition board. The sample library is arranged in the sample storage room. The sample library is used for freezing and storing samples. The sample storage room provides a low-temperature environment for the sample library and is a transition storage room for sample transfer. A sample transfer window is arranged on the outer side wall of the sample storage room at the position of the sample storage room. A sample transmission mechanism for transferring samples between the sample transfer window and the sample library is also arranged in the sample storage room. An inverse Brayton cycle refrigeration device and a refrigeration and dehumidification device are arranged in the equipment room. The sample library is connected with an air inlet pipe and an air return pipe, and the air inlet pipe and the air return pipe penetrate through the heat-insulated partition board and are respectively connected with the air supply pipe orifice and the air return pipe orifice of the inverse Brayton cycle refrigeration device. The refrigeration and dehumidification device is connected with the sample storage room through a pipe penetrating through the heat-insulated partition board. A temperature and humidity sensor is arranged in the sample storage room, and a temperature sensor is arranged in the sample library. The inverse Brayton cycle refrigeration device provides cold quantity for the sample library through the air inlet pipe, recovers the cold quantity through the air return pipe. The refrigeration and dehumidification device provides cold quantity into the sample storage room, and the internal temperature of the sample storage room is controlled at -25°C by the refrigeration and dehumidification device. At the same time, the internal humidity of the sample storage room is kept below the dew point under the control of the refrigeration and dehumidification device, preventing the surface of the sample freezing container from frosting and affecting the code scanning and reading, and preventing the outer wall surface of the sample library from condensing and icing. And the internal temperature of the sample library is lower than the internal temperature of the sample storage room.

2. The automated biological sample cold storage based on reverse Brayton cycle refrigeration according to claim 1, wherein: The sample library includes a sample storage library body. The sample storage library body includes a heat-insulated top plate and a heat-insulated bottom plate which are distributed up and down. Heat-insulated side plates are arranged between the two side ends of the heat-insulated top plate and the heat-insulated bottom plate. An air inlet and an air outlet are arranged on one of the heat-insulated side plates, and the air inlet pipe and the air return pipe are respectively connected with the air inlet and the air outlet. A heat-insulated back plate is arranged between the backs of the heat-insulated top plate and the heat-insulated bottom plate. A storage shelf is arranged in the sample storage library body.

3. The automated biological sample cold storage based on reverse Brayton cycle refrigeration according to claim 2, wherein: The storage shelf includes a support frame. A plurality of sample tray drawers which can slide back and forth along the sample storage library body are arranged in the support frame. The push-pull end plate on the front side of the sample tray drawer is a pull-out heat-insulated door.

4. The automated biological sample cold storage based on reverse Brayton cycle refrigeration according to claim 2, wherein: The heat-insulated top plate, the heat-insulated bottom plate, the heat-insulated side plates and the heat-insulated back plate are provided with polyurethane heat-insulating layers.

5. The automated biological sample cold storage based on reverse Brayton cycle refrigeration according to claim 2, characterized in that: One ends of the air inlet pipe and the air return pipe both horizontally penetrate into the sample storage library body, and the lengths of the air inlet pipe and the air return pipe penetrating into the sample storage library body are adapted to the length of the internal space of the sample storage library body. A plurality of air holes are uniformly arranged on the pipe walls of the air inlet pipe and the air return pipe penetrating into the sample storage library body.

6. The automated biological sample cold storage based on reverse Brayton cycle refrigeration according to claim 1, wherein: A storage room maintenance door and a central control screen are also arranged on the outer side wall of the heat-insulated storage body at the position of the sample storage room. An equipment room maintenance door is arranged on the outer wall of the heat-insulated storage body at the position of the equipment room.

7. An automated biological sample cold storage based on reverse Brayton cycle refrigeration according to claim 1, characterized in that: An outer heat-insulated door is arranged on the outer side wall of the heat-insulated storage body at the position corresponding to the sample transfer window, and an inner heat-insulated door is arranged on the inner side wall of the heat-insulated storage body at the position corresponding to the sample transfer window.

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