Fermentation detection method for eurotium cristatum in Fuzhuan tea based on liquid chromatography

Abstract

The invention relates to the technical field of liquid chromatography detection, in particular to a fermentation detection method for eurotium cristatum in Fuzhuan tea based on liquid chromatography. The method comprises the following steps: extracting a chromatographic curve of a sample; determining the offset possibility according to the distance and height of the spectrum peak of the chromatographic curve, determining the offset degree of the spectrum peak by combining the width of the spectrum peak, and averaging to obtain the offset degree of the chromatographic curve of the sample; determining the spectrum peak offset difference according to the offset degree difference of the spectrum peaks of one component and all other components in the sample and the position difference of different samples with the same component; an error coefficient is determined on the basis of spectrum peak deviation difference, the deviation degree of the chromatographic curve is corrected, and then the chromatographic curve is corrected; and calculating the component concentration based on the corrected chromatographic curve so as to complete fermentation detection. According to the method, the spectrum peak is corrected, and the fermentation detection precision is improved.

Description

Technical Field

[0001] This application relates to the technical field of liquid chromatography, and specifically to a method for detecting the fermentation of Eurotium cristatum in Fuzhuan tea based on liquid chromatography. Background Art

[0002] The fermentation of Eurotium cristatum in Fuzhuan tea is an important source of its unique flavor and health value. During the production process of Fuzhuan tea, the introduction and cultivation of Eurotium cristatum can promote the transformation of organic substances in the tea, producing unique flavor components and nutritional components. Its unique fermentation process enables the better release of the nutritional components in the tea, enhancing the health experience of drinkers. Most traditional methods for detecting Eurotium cristatum rely on microbial culture techniques, which are not only time-consuming but also require a relatively high operating environment. Liquid chromatography technology, especially high-performance liquid chromatography (HPLC), has gradually become an important tool for detecting the fermentation process of Eurotium cristatum in Fuzhuan tea due to its high separation efficiency and sensitivity. Through liquid chromatography, the metabolites related to the fermentation of Eurotium cristatum in the tea, such as polyphenolic compounds and amino acids, can be effectively separated and quantified, thereby evaluating the degree and quality of fermentation.

[0003] When measuring the content of metabolites in the fermentation process of Eurotium cristatum in Fuzhuan tea by high-performance liquid chromatography, since there are many components of the metabolites produced by fermentation that are relatively similar, such as various glutamic acid compounds, polyphenolic compounds, etc., the distribution of each component in the obtained liquid chromatography image is relatively dense, resulting in the phenomenon of peak tailing; then when determining the types and contents of the components, they will affect each other, affecting the accuracy of component determination. Summary of the Invention

[0004] In order to solve the technical problem of low detection accuracy caused by dense component distribution, this application provides a method for detecting the fermentation of Eurotium cristatum in Fuzhuan tea based on liquid chromatography. The specific technical solutions adopted are as follows: This application proposes a method for detecting the fermentation of Eurotium cristatum in Fuzhuan tea based on liquid chromatography. The method includes the following steps: Collect samples at different fermentation stages, extract the samples, and then use chromatography to process them to obtain the chromatographic curve of the samples; Determine the possibility of peak offset based on the distance between adjacent peaks and the height of the peaks in the chromatographic curve; obtain the width and height of the peaks, determine the degree of peak offset based on the ratio of the width and height and the possibility of offset; obtain the degree of offset of the sample chromatographic curve based on the mean value of the degree of offset and width of all peaks; Determine the spectral peak offset difference of each component according to the difference in the offset degree between the spectral peaks corresponding to each component of the sample and all the spectral peaks in the sample, and the difference in the positions of the spectral peaks of different samples under each component; determine the error coefficient according to the difference between the spectral peak offset difference of each component and the spectral peak offset differences of all components; correct the offset degree of the sample chromatogram curve based on the error coefficient, and then correct the chromatogram curve. Obtain the peak area of the spectral peak based on the corrected chromatogram curve, compare the sample with the standard product, calculate the concentration of the component based on the peak area of the spectral peak, and then complete the fermentation detection.

[0005] In the above solution, the present application first sets the chromatographic column to obtain the chromatogram curve of the sample. For the phenomenon of peak tailing and other phenomena of the spectral peak, determine the offset possibility based on the height and distance of the spectral peak, and then calculate the offset degree of the chromatogram curve based on the offset possibility; thus determine the overall offset situation of the spectral peak; also, since the different component contents in different stages may cause the offset situation not to reflect the overall offset of the spectral peak, the error coefficient is determined by combining the spectral peak offsets of different components in different stages. After correcting the spectral peak offset coefficient, the curve is corrected, and then the concentration is calculated to complete the fermentation detection; among them, by controlling variables, calculating the overall spectral peak offset based on the spectral peak offset degrees of the same component in different stages and different components in the same stage is more accurate, making the final fermentation detection effect more accurate.

[0006] In one embodiment, the column temperature of the chromatographic column selected in the chromatography method is set to 30 - 35 °C, the flow rate is 0.8 - 1.0 mL / min, and the injection volume is 10 - 20 μL.

[0007] In one embodiment, the offset possibility of the spectral peak is inversely proportional to the distance between adjacent spectral peaks and directly proportional to the height of the spectral peak.

[0008] In one embodiment, the width of the spectral peak is the distance between the leftmost and rightmost points of the spectral peak, and the height of the spectral peak is the maximum value of the spectral peak.

[0009] In one embodiment, the method for determining the offset degree of the spectral peak based on the ratio of the width and height and the offset possibility is as follows: Denote the ratio of the width of the spectral peak to the height of the spectral peak as the first ratio, and take the product of the first ratio and the offset possibility of the spectral peak as the offset degree of the spectral peak.

[0010] In one embodiment, the method for obtaining the offset degree of the chromatogram curve according to the mean value of the offset degrees and widths of all spectral peaks is as follows: , represents the offset degree of the j-th spectral peak, represents the number of spectral peaks, represents the mean value of the widths of all spectral peaks, Indicates the degree of offset of the sample.

[0011] In one embodiment, the spectral peak offset difference is positively correlated with the offset degree difference and the position difference respectively.

[0012] In one embodiment, the method for obtaining the error coefficient by determining the difference between the spectral peak offset difference of each component and the spectral peak offset difference of all components is as follows: , represents the spectral peak offset difference of the r-th component, represents the mean of the spectral peak offset differences of all components, represents the number of components, represents the spectral peak error coefficient.

[0013] In one embodiment, the method for correcting the offset degree of the sample chromatogram curve based on the error coefficient and then correcting the chromatogram curve is as follows: , represents the offset degree of the chromatogram curve of the sample to be detected, represents the linear normalization function, represents the spectral peak error coefficient, represents the offset degree of the corrected sample chromatogram curve; The product of the width of each spectral peak and the offset degree of the corrected sample chromatogram curve is used as the width of the corrected chromatogram curve, thereby completing the correction of the chromatogram curve.

[0014] In one embodiment, the method for calculating the concentration of a component by comparing a sample with a standard product based on the peak area of the spectral peak is as follows: Plot a standard curve with different concentrations of the standard product and their corresponding peak areas; obtain the intercept and slope of the standard curve; The expression for the concentration of the sample component is: , represents the peak area of each component corresponding spectral peak in the sample, represents the intercept of the standard curve, represents the slope of the standard curve, represents the concentration of the component.

[0015] The beneficial effects of this application are: In this application, the chromatographic column is first set to obtain the chromatogram of the sample. For the phenomenon of peak tailing in the chromatogram, the offset possibility is determined based on the height and distance of the peaks. Then, the offset degree of the chromatogram is calculated based on the offset possibility, so as to determine the overall offset situation of the peaks. Also, since the different component contents in different stages may cause the offset situation not to reflect the overall offset of the peaks, the error coefficient is determined by combining the peak offsets of different components in different stages. After correcting the peak offset coefficient, the curve is corrected, and then the concentration is calculated to complete the fermentation detection. Among them, by controlling variables, calculating the overall peak offset based on the peak offset degrees of the same component in different stages and different components in the same stage is more accurate, making the final fermentation detection effect more accurate. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] To more clearly illustrate the technical solutions and advantages in the embodiments of the present application or the prior art, the following will briefly introduce the drawings required for the description of the embodiments or the prior art. Obviously, the drawings in the following description are only some embodiments of the present application. For those of ordinary skill in the art, other drawings can be obtained based on these drawings without creative efforts.

[0017] Figure 1 It is a flowchart of a method for detecting the fermentation of Eurotium cristatum in Fuzhuan tea based on liquid chromatography provided by an embodiment of the present application. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0018] To further elaborate on the technical means and effects adopted by the present application to achieve the intended invention purpose, the following will describe in detail the specific implementation manner, structure, characteristics and effects of the method for detecting the fermentation of Eurotium cristatum in Fuzhuan tea based on liquid chromatography proposed according to the present application in combination with the drawings and preferred embodiments. In the following description, different "one embodiment" or "another embodiment" do not necessarily refer to the same embodiment. In addition, the specific features, structures or characteristics in one or more embodiments can be combined in any suitable form.

[0019] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by those skilled in the technical field to which this application belongs.

[0020] Embodiment of the method for detecting the fermentation of Eurotium cristatum in Fuzhuan tea based on liquid chromatography: The following will specifically describe the specific solution of the method for detecting the fermentation of Eurotium cristatum in Fuzhuan tea based on liquid chromatography provided by the present application in combination with the drawings.

[0021] Please refer to Figure 1 , which shows a flowchart of a method for detecting the fermentation of Eurotium cristatum in Fuzhuan tea based on liquid chromatography provided by an embodiment of the present application. The method includes the following steps: Step S001: Collect a sample and obtain its chromatogram curve.

[0022] Collect representative samples from different stages of Fuzhuan tea fermentation (such as before piling, initial stage of flower formation, maturity stage, etc.), quickly freeze-dry them, crush them to less than 60 mesh, and store them at low temperature in the dark to reduce component degradation.

[0023] Extract different components from the crushed samples. In this embodiment, organic acids, polyphenols, and flavonoids are described. After extracting the samples, each component is processed by HPLC (High Performance Liquid Chromatography), and the chromatogram curve of the sample is obtained through the chromatographic column and detector. The abscissa of the chromatogram curve is time, and the ordinate is the signal response value.

[0024] The column temperature of the selected chromatographic column is set at 30 - 35 °C, the flow rate is 0.8 - 1.0 mL / min, and the injection volume is 10 - 20 μL.

[0025] So far, the chromatogram curve of the sample in Fuzhuan tea has been obtained.

[0026] Step S002: Determine the possibility of peak shift based on the distance and height of the chromatogram peaks, and then combine the peak width to determine the degree of peak shift. Take the average value to obtain the degree of peak shift of the sample chromatogram curve.

[0027] The "flowering bacteria" fermentation of Fuzhuan tea is its core process, which is dominated by Eurotium cristatum. In the pressed tea bricks, by controlling the temperature, humidity, and tightness of the bricks, the spores of Eurotium cristatum are promoted to germinate and form golden ascocarps. During fermentation, the bacteria secrete substances such as amylase and oxidase, which catalyze the oxidation of polyphenols in tea leaves to form theaflavins, and at the same time decompose starch into monosaccharides, giving the tea soup a mellow and sweet "bacterial flower fragrance" characteristic. The metabolic activities of Eurotium cristatum can also transform the astringency of coarse and old tea leaves and produce the probiotic function of regulating the intestinal flora, and its content is positively correlated with the quality of Fuzhuan tea.

[0028] Therefore, it is crucial to detect Eurotium cristatum during the fermentation process of Fuzhuan tea. The quantity and activity of Eurotium cristatum directly affect the sensory quality of Fuzhuan tea. The enzyme substances produced by its metabolism can transform components such as tea polyphenols and starch, forming a unique "bacterial flower fragrance" and mellow taste. Detection can ensure that the fermentation degree meets the standard. Secondly, if the temperature and humidity are out of control during the fermentation environment, miscellaneous bacteria (such as Aspergillus niger and Penicillium) may grow, and detection can timely identify the pollution risk and avoid the harm of toxic metabolites to consumers' health. In addition, the probiotic function of Eurotium cristatum (such as lipid-lowering and blood sugar regulation) is closely related to its viable bacteria count. Quantitative detection can verify the health care value of the product and provide data support for process optimization.

[0029] When detecting the Eurotium cristatum and its products during the fermentation process of Fuzhuan tea, since there are many components in the metabolites produced by fermentation that are relatively similar, such as various glutamic acid compounds, polyphenolic compounds, etc., the distribution of each component in the obtained liquid chromatography image is relatively dense, resulting in the phenomenon of peak tailing.

[0030] Glutamic acid compounds (such as glutamine, N-acetylglutamine) and polyphenolic substances (such as tea polyphenols and their oxidation product theabrownin) have similar polar groups, and their retention times overlap on a reverse-phase chromatographic column, resulting in peak broadening and tailing; the amylase, cellulase, etc. secreted by Eurotium cristatum continuously decompose the substrate, making intermediate metabolites (such as monosaccharide derivatives) coexist with end products. The ultraviolet absorption characteristics of these substances are similar (such as the absorption overlap of tea polyphenols and flavonoids at 280 nm), resulting in baseline elevation and peak shoulder interference.

[0031] Therefore, if the peak changes, it will cause the retention time of the compound to deviate from the expected value, interfering with qualitative judgment, and if the peak shifts, it may cause the originally separated component peaks to overlap, reducing the chromatographic resolution.

[0032] Therefore, in order to accurately analyze the measured components, it is necessary to correct the offset of the obtained high-performance liquid chromatography data; when performing peak correction, it is necessary to analyze the changes in the peaks at different fermentation stages, obtain the degree of peak offset, and then perform correction according to the degree of offset.

[0033] For any fermentation stage, obtain the chromatographic curve of the sample at this fermentation stage, obtain the peaks in the chromatographic curve. If the distance between one peak and its adjacent peak is closer, and the height of this peak is higher, then this peak is more likely to be interfered. Therefore, determine the offset possibility of the peak based on the distance between adjacent peaks and the height of the peak.

[0034] The offset possibility of the peak is inversely proportional to the distance between adjacent peaks and directly proportional to the height of the peak.

[0035] It should be noted that positive correlation means that when one variable increases, the other variable also increases, and the change directions of the two variables are the same. When one variable changes from large to small or from small to large, the other variable also changes from large to small or from small to large; the specific relationship is determined by actual applications, and this application does not make special restrictions.

[0036] Preferably, in this embodiment, the expression of the offset possibility is: , represents the abscissa of the maximum value of the j-th peak, represents the abscissa of the maximum value of the (j + 1)-th peak, represents the peak value of the j-th peak, represents the linear normalization function, Indicates the offset possibility of the j-th spectral peak.

[0037] Among them, the spectral peak with offset will affect the shapes of other spectral peaks, increase the widths of other spectral peaks, reduce the steepness of the spectral peaks, and change the shapes of adjacent spectral peaks; if there is no offset, the spectral peak has a greater steepness and its shape will not change significantly.

[0038] For each spectral peak, the leftmost and rightmost points are taken as the minimum points of the spectral peak, the distance between these two points is taken as the width of the spectral peak, and the maximum value of the spectral peak is taken as the height of the spectral peak; based on the ratio of the width to the height of the spectral peak and the offset possibility, the offset degree of the spectral peak is determined.

[0039] Preferably, the expression for the offset degree of the spectral peak is: , represents the width of the j-th spectral peak, represents the height of the j-th spectral peak, represents the offset possibility of the j-th spectral peak, represents the offset degree of the j-th spectral peak.

[0040] Based on the offset degrees of all spectral peaks, the offset degree of the sample can be estimated. The expression for the offset degree of the chromatogram curve is: , represents the offset degree of the j-th spectral peak, represents the number of spectral peaks, represents the average width of all spectral peaks, represents the offset degree of the chromatogram curve.

[0041] Among them, the higher the spectral peak, the greater the offset degree if it is offset; while the lower the spectral peak, the smaller the offset degree if it is offset, and after offset, the peak width will increase; therefore, the ratio of the peak width to the peak height is used to reflect the offset degree, and the larger the ratio, the greater the offset degree; and the offset occurs to the entire liquid chromatography data, so the average offset degree of all spectral peaks is calculated and used as the offset degree of the sample chromatogram curve.

[0042] Thus, the offset degree of the sample chromatogram curve is obtained.

[0043] Step S003: Determine the spectral peak offset difference based on the offset degree difference between one component and the spectral peaks of all other components in the sample and the position difference of the same component in different samples; then determine the error coefficient based on the spectral peak offset difference, correct the offset degree of the chromatogram curve, and further correct the chromatogram curve.

[0044] The offset degree of the sample is calculated through the above steps. However, during different fermentation periods of Fuzhuan tea, the contents of the fermentation products of Eurotium cristatum are different. Substances with different contents have different peak heights and widths in different liquid chromatography data, and the offset degrees generated by different contents in the liquid chromatography data are different. Therefore, it is necessary to compare the differences in the liquid chromatography data of the samples detected during different fermentation periods to obtain an accurate offset degree.

[0045] For all samples, observe the chromatographic column to obtain the corresponding relationship of the peaks of the same components in different samples; determine the peak offset difference of each component based on the offset degree difference between the peak of this component corresponding to the sample itself and all the peaks in this sample, as well as the position difference of the peaks of different samples under each component.

[0046] The peak offset difference is positively correlated with the offset degree difference and the position difference respectively.

[0047] Preferably, in this embodiment, the expression of the peak offset difference is: , represents the abscissa of the peak maximum of the r-th component corresponding to the a-th sample, represents the abscissa of the peak maximum of the r-th component corresponding to the b-th sample, represents the offset degree of the peak corresponding to the r-th component in the b-th sample, represents the mean value of the offset degrees of the peaks corresponding to the r-th component of all samples, represents the number of samples, represents the peak offset difference of the r-th component.

[0048] Specifically, represents the difference of the same component in different chromatographic curves. The abscissa of the chromatographic curve represents the change in the composition state of the mobile phase corresponding to each time point in the chromatographic system, reflecting the migration process of the components in the chromatographic column. If there is a difference in the abscissa, it means that there is a peak offset when obtaining the chromatographic curve. Therefore, compare the differences of the same component in different chromatographic data here. The larger the difference value, the greater the peak offset difference; represents the difference value of the offset degrees of the same peaks in different samples. The larger the difference value, the greater the peak offset difference.

[0049] According to the peak offset difference of the same component in different samples obtained above, correct the peak offset degree. Since the peak offset is an overall offset, only the offset degrees of components with different contents are different. Therefore, obtain the error coefficient according to the peak offset difference obtained above, and then make corrections according to the error coefficient.

[0050] The expression of the error coefficient is as follows: , represents the spectral peak offset difference of the r-th component, represents the mean of the spectral peak offset differences of all components, represents the number of components, represents the spectral peak error coefficient.

[0051] According to the error coefficient, the offset degree of the sample is corrected. The larger the error coefficient, the greater the degree of correction required. And since the spectral peak width will increase after the spectral peak offset, the correction is also to reduce the spectral peak width. Based on this, the spectral peak offset coefficient is corrected, and the expression is: , represents the offset degree of the chromatographic curve of the sample to be detected, represents the linear normalization function, represents the spectral peak error coefficient, represents the offset degree of the chromatographic curve of the sample after correction.

[0052] Based on the offset degree of the sample after correction, the chromatographic curve is corrected. The product of the width of each spectral peak and the offset degree of the chromatographic curve of the sample after correction is used as the width of the chromatographic curve after correction. That is, the correction of the chromatographic curve is completed.

[0053] So far, the correction of the chromatographic curve has been completed.

[0054] Step S004, calculate the component concentration based on the corrected chromatographic curve, and then complete the fermentation detection.

[0055] Through the above steps, the correction of the sample spectral peak curve is completed. Then, the reference standards at different fermentation stages are found, and the different concentrations of the reference standards and their corresponding peak areas are plotted on a graph to obtain the standard curve. Usually, linear regression analysis is used to fit the data. The peak areas of each target component in the sample are extracted from the HPLC data.

[0056] Then, based on the intercept, slope of the standard curve, and the peak area of each component of the sample, the concentration of each component in the sample is calculated. The formula for calculating the concentration is: , represents the peak area of the spectral peak corresponding to each component in the sample, represents the intercept of the standard curve, represents the slope of the standard curve, represents the concentration of the component.

[0057] The concentrations of different components in the sample are calculated therefrom. Then, different concentration conditions are set for different components at different fermentation stages based on the standard products and existing standards, and it is determined whether the current component concentration meets the concentration conditions. If it meets, it indicates that the fermentation degree of the sample is good; if it does not meet, it indicates that the fermentation degree is poor. Based on this, the fermentation detection is completed.

[0058] It should be noted that the above-described embodiments are only used to illustrate the technical solutions of the present application, rather than to limit them; although the present application has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that they can still modify the technical solutions described in the foregoing embodiments, or perform equivalent replacements on some of the technical features; and these modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the various embodiments of the present application, and should all be included in the protection scope of the present application.

[0059] Each embodiment in this specification is described in a progressive manner, and the same or similar parts between the embodiments can be referred to each other. The key point of each embodiment is to illustrate the differences from other embodiments.

Claims

1. A method for detecting the fermentation of Eurotium cristatum in Fuzhuan tea based on liquid chromatography, characterized in that, The method includes the following steps: Collect samples at different fermentation stages, extract the samples, and then use chromatography to process them to obtain the chromatographic curves of the samples; Determine the offset possibility of the peaks based on the distance between adjacent peaks and the height of the peaks in the chromatographic curve; obtain the width and height of the peaks, and determine the offset degree of the peaks based on the ratio of the width and height and the offset possibility; obtain the offset degree of the sample chromatographic curve according to the mean value of the offset degrees and widths of all peaks; Determine the peak offset difference of each component according to the difference in the offset degree between the peaks corresponding to each component of the sample and all the peaks in the sample and the difference in the peak positions of different samples under each component; determine the error coefficient according to the difference between the peak offset difference of each component and the peak offset differences of all components; correct the offset degree of the sample chromatographic curve based on the error coefficient, and then correct the chromatographic curve; Obtain the peak area of the peaks based on the corrected chromatographic curve, compare the sample with the standard product, calculate the concentration of the components based on the peak area of the peaks, and then complete the fermentation detection.

2. The method for detecting the fermentation of Eurotium cristatum in Fu brick tea based on liquid chromatography according to claim 1, wherein, In the chromatography, the column temperature of the selected chromatographic column is set at 30 - 35 °C, the flow rate is 0.8 - 1.0 mL / min, and the injection volume is 10 - 20 μL.

3. The method for detecting the fermentation of Eurotium cristatum in Fuzhuan tea based on liquid chromatography according to claim 1, characterized in that, The offset possibility of the peaks is inversely proportional to the distance between adjacent peaks and directly proportional to the height of the peaks.

4. The method for detecting the fermentation of Eurotium cristatum in Fuzhuan tea based on liquid chromatography according to claim 1, wherein The width of the peak is the distance between the leftmost and rightmost points of the peak, and the height of the peak is the maximum value of the peak.

5. The method for detecting the fermentation of Eurotium cristatum in Fuzhuan tea based on liquid chromatography according to claim 1, wherein The method for determining the offset degree of the peaks based on the ratio of the width and height and the offset possibility is as follows: Denote the ratio of the width of the peak to the height of the peak as the first ratio, and take the product of the first ratio and the offset possibility of the peak as the offset degree of the peak.

6. The method for detecting the fermentation of Eurotium cristatum in Fuzhuan tea based on liquid chromatography according to claim 1, wherein The method for obtaining the offset degree of the chromatographic curve according to the mean value of the offset degrees and widths of all peaks is as follows: , represents the deviation degree of the j-th spectral peak, represents the number of spectral peaks, represents the average width of all spectral peaks, represents the deviation degree of the sample.

7. The method for detecting the fermentation of Eurotium cristatum in Fuzhuan tea based on liquid chromatography according to claim 1, wherein The peak offset difference is positively correlated with the offset degree difference and the position difference respectively.

8. The method for detecting the fermentation of Eurotium cristatum in Fuzhuan tea based on liquid chromatography according to claim 1, wherein The method for determining the error coefficient according to the difference between the peak offset difference of each component and the peak offset differences of all components is as follows: , represents the spectral peak shift difference of the r-th component, represents the mean value of the spectral peak shift differences of all components, represents the number of components, represents the spectral peak error coefficient.

9. The method for detecting the fermentation of Eurotium cristatum in Fuzhuan tea based on liquid chromatography according to claim 1, characterized in that, The method for correcting the offset degree of the sample chromatographic curve based on the error coefficient and then correcting the chromatographic curve is as follows: , represents the deviation degree of the chromatogram curve of the sample to be detected, represents the linear normalization function, represents the spectral peak error coefficient, represents the deviation degree of the chromatogram curve of the corrected sample; Take the product of the width of each peak and the offset degree of the corrected sample chromatographic curve as the width of the corrected chromatographic curve, thereby completing the correction of the chromatographic curve.

10. The method for detecting the fermentation of Eurotium cristatum in Fuzhuan tea based on liquid chromatography according to claim 1, wherein The method for comparing the sample with the standard product and calculating the concentration of the components based on the peak area of the peaks is as follows: Plot the standard curve of different concentrations of the standard product and their corresponding peak areas; obtain the intercept and slope of the standard curve; The expression for the concentration of the sample component is: , represents the peak area of each component corresponding spectral peak in the sample, represents the intercept of the standard curve, represents the slope of the standard curve, represents the concentration of the component.

Images