Method for detecting and identifying formula granules of rice-grain sprouts and processed products of rice-grain sprouts

Abstract

The invention relates to the technical field of traditional Chinese medicine detection and identification, in particular to a detection and identification method for rice-grain sprout and processed product formula granules thereof. The identification method for the formula granules of the rice-grain sprouts and the processed products of the rice-grain sprouts comprises the following steps: obtaining test solution of the rice-grain sprouts and the processed products of the rice-grain sprouts; performing ultra-high performance liquid chromatography detection on the test solution to obtain a liquid chromatogram containing characteristic peaks corresponding to 4-coumaric acid and 5-hydroxymethylfurfural; wherein a characteristic peak corresponding to 5-hydroxymethylfurfural is absent in a liquid chromatogram of the rice sprout formula granules; in a liquid chromatogram of the fried rice sprout formula granules, the peak area ratio of 5-hydroxymethylfurfural to 4-coumaric acid is in a range of 0.21-0.87; the peak area ratio of 5-hydroxymethylfurfural to 4-coumaric acid in a liquid chromatogram of the scorched rice sprout formula granules is in a range of 1.49 to 3.57. The identification method disclosed by the invention can be used for effectively distinguishing the rice-grain sprouts and the processed product formula granules thereof.

Description

Technical Field

[0001] The invention relates to the technical field of chromatographic analysis of traditional Chinese medicine formula particles, and in particular to a method for detecting and identifying formula particles of rice sprouts and processed products thereof. Background Art

[0002] Rice sprouts are processed products of the mature fruits of the grass plant rice (Oryza sativa L.) that have been germinated and dried. There are many methods for processing rice sprouts. According to the different processing methods, rice sprouts are divided into rice sprouts (also called raw rice sprouts), fried rice sprouts and burnt rice sprouts. The processing methods of rice sprouts, fried rice sprouts and burnt rice sprouts specified in the "Pharmacopoeia of the People's Republic of China (2020 Edition)" are as follows: Rice sprouts: Soak the rice grains in water, maintain appropriate temperature and humidity, and dry them when the fibrous roots grow to about 1 cm; Fried rice sprouts: Take clean rice sprouts and stir-fry them according to the stir-fry method (General Rule 0213) until they are dark yellow; Burnt rice sprouts: Take clean rice sprouts and stir-fry them according to the stir-fry method (General Rule 0213) until they are burnt yellow. Different processing methods lead to different effects of different processed rice sprouts. For example, rice sprouts can help digestion and harmonize the stomach, strengthen the spleen and stimulate the appetite, and are used for indigestion, abdominal distension and bad breath, weak spleen and stomach, and less food when not hungry. Fried rice sprouts tend to help digestion and are used for less food when not hungry. Scorched rice sprouts are good at dissolving stagnation and are used to treat stagnation. Different preparations of rice sprouts have different emphases on the digestion effect and cannot be replaced by each other.

[0003] Chinese medicine formula granules are granules made from single Chinese medicine slices through water heating extraction, separation, concentration, drying and granulation. Under the guidance of Chinese medicine theory, they are prepared according to Chinese medicine clinical prescriptions for patients to take with water. The product maintains the nature, taste and efficacy of Chinese medicine slices, with stable and reliable quality. It is used in the preparation of Chinese medicine clinical prescriptions, adapting to the needs of dialectical treatment and prescription changes, and has the advantages of no need for decoction, convenient to take, accurate dosage, safe and clean, and easy to carry. Therefore, Chinese medicine formula granules have been rapidly applied in clinical practice, and the usage in some medical institutions has exceeded that of traditional slices. Rice sprout formula granules, fried rice sprout formula granules and burnt rice sprout formula granules are all available on the market. However, because Chinese medicine formula granules extract and concentrate traditional Chinese medicine slices, they have lost the appearance characteristics and microscopic characteristics of traditional Chinese medicine slices, and their characteristics such as smell are not easy to identify and distinguish, so it is difficult to distinguish them by properties and microscopic identification. This is the inconvenience of Chinese medicine formula granules compared with traditional slices. However, there is currently a lack of rapid and effective methods to detect, identify and differentiate rice sprout formula granules, fried rice sprout formula granules and burnt rice sprout formula granules. This will be detrimental to the identification and differentiation of finished products as well as market supervision for formula granules that lack appearance and microscopic differences. Summary of the invention

[0004] Therefore, the technical problem to be solved by the present invention is to overcome the defect that the prior art lacks an effective method for detecting, identifying and differentiating rice sprouts and their processed formula granules, so as to provide a detection and identification method for rice sprouts and their processed formula granules to solve the above problems.

[0005] To achieve the above object, the present invention provides the following technical solutions:

[0006] An identification method for rice sprouts and their processed formula granules, comprising the following steps:

[0007] Obtain the test solution of rice sprouts and their processed products; perform ultra-high performance liquid chromatography (UHPLC) detection on the test solution to obtain a liquid chromatogram containing characteristic peaks corresponding to 4-coumaric acid and 5-hydroxymethylfurfural;

[0008] The rice sprouts and their processed formula granules include: stir-fried rice sprout formula granules, charred rice sprout formula granules, and rice sprout formula granules;

[0009] The liquid chromatogram of the rice sprout formula granules lacks the characteristic peak corresponding to 5-hydroxymethylfurfural;

[0010] In the liquid chromatogram of the stir-fried rice sprout formula granules, the peak area ratio of 5-hydroxymethylfurfural to 4-coumaric acid is in the range of 0.21 - 0.87;

[0011] In the liquid chromatogram of the charred rice sprout formula granules, the peak area ratio of 5-hydroxymethylfurfural to 4-coumaric acid is in the range of 1.49 - 3.57.

[0012] Furthermore, in the liquid chromatograms of the stir-fried rice sprout formula granules and the charred rice sprout formula granules, there is also a characteristic peak peak 1 with a relative retention time of 0.71 - 0.79 relative to the characteristic peak corresponding to 5-hydroxymethylfurfural, and the liquid chromatogram of the rice sprout formula granules lacks peak 1.

[0013] The present invention also provides a detection method for rice sprouts and their processed formula granules for the above identification method. The detection method is ultra-high performance liquid chromatography, and the detection conditions of the ultra-high performance liquid chromatography include: octadecylsilane-bonded silica gel as the filler, the detection wavelength is 295 - 305 nm, acetonitrile is used as mobile phase A, and an aqueous solution of formic acid with a volume percentage of 0.08 - 0.12% is used as mobile phase B;

[0014] Perform elution according to the following gradient program:

[0015]

[0016] The liquid chromatogram obtained by the ultra-high performance liquid chromatography detection includes a characteristic peak peak 3 corresponding to 4-coumaric acid, a characteristic peak peak 2 corresponding to 5-hydroxymethylfurfural, and a characteristic peak peak 1 with a relative retention time of 0.71 - 0.79 relative to peak 2.

[0017] Preferably, in the conditions for detection by ultra - performance liquid chromatography, the column length of the chromatographic column is 150 mm, the inner diameter is 2.1 mm, and the particle size is 1.8 μm.

[0018] Preferably, in the conditions for detection by ultra - performance liquid chromatography, the flow rate is 0.33 - 0.37 ml / min, preferably 0.35 ml / min.

[0019] Preferably, in the conditions for detection by ultra - performance liquid chromatography, the column temperature is 32 - 38 °C, preferably 35 °C.

[0020] Preferably, in the conditions for detection by ultra - performance liquid chromatography, the injection volume is 1 - 3 μl, preferably 2 μl.

[0021] Preferably, in the conditions for detection by ultra - performance liquid chromatography, the number of theoretical plates calculated based on the 4 - coumaric acid peak should be not less than 60000.

[0022] Preferably, the preparation process of the test solution is as follows: Take the test sample, grind it finely, weigh it precisely, add the solvent, weigh the weight, perform extraction treatment, take it out, let it cool, weigh the weight again, make up the lost weight with the corresponding solvent, shake well, filter, and take the subsequent filtrate to obtain the test solution.

[0023] Preferably, the extraction treatment is ultrasonic treatment;

[0024] And / or, the duration of the extraction treatment is 20 - 40 min, preferably 30 min;

[0025] And / or, the solvent is an aqueous methanol solution with a volume percentage of 60 - 80%, preferably an aqueous methanol solution with a volume percentage of 70%;

[0026] And / or, relative to 10 - 30 ml of the solvent, the addition amount of the test sample is 0.1 - 0.3 g, preferably 0.2 g of the test sample is added corresponding to 20 ml of the solvent.

[0027] In the present invention, unless otherwise specified, the percentages of solutions are all volume percentages.

[0028] The technical solution of the present invention has the following advantages:

[0029] 1. A method for identifying germinated rice and its processed product formula granules. Qualitative analysis can be carried out according to the presence or absence of the chromatographic peak corresponding to 5-hydroxymethylfurfural in the measured liquid chromatogram and the ratio of the relative peak areas of the characteristic peaks corresponding to 5-hydroxymethylfurfural and 4-coumaric acid, which can effectively identify germinated rice and its processed product formula granules. Specifically, only two characteristic peaks corresponding to 5-hydroxymethylfurfural and 4-coumaric acid need to be located in the liquid chromatogram of the present invention. Through the presence or absence of the peaks and the ratio of the relative peak areas, germinated rice formula granules, stir-fried germinated rice formula granules, and scorched germinated rice formula granules can be accurately distinguished.

[0030] 2. A method for detecting germinated rice and its processed product formula granules, comprising the following steps: preparation of a test solution; ultra-high performance liquid chromatography detection of the test solution; the conditions for the ultra-high performance liquid chromatography detection include: octadecylsilane-bonded silica gel as the filler, the detection wavelength is 295 - 305 nm, acetonitrile as mobile phase A, and a formic acid aqueous solution with a volume percentage of 0.08 - 0.12% as mobile phase B; elution is carried out according to a specific gradient program. This detection method can effectively show the characteristic components of germinated rice and its processed products in the liquid chromatogram, providing a prerequisite guarantee for the identification of germinated rice and its processed product formula granules.

[0031] 3. A method for detecting germinated rice and its processed product formula granules. The obtained chromatogram of the method of the present invention has good resolution, there is no interference between peaks, and both peak 2 and peak 3 are known components, without the situation of being difficult to identify. It does not require quantitative characterization and calculation with a reference substance, and the data does not need to be imported into software for secondary analysis, saving time and effort, with low difficulty and stronger practicability. It can quickly, accurately, and stably distinguish germinated rice formula granules, stir-fried germinated rice formula granules, and scorched germinated rice formula granules. Description of the Drawings

[0032] In order to more clearly illustrate the specific embodiments of the present invention or the technical solutions in the prior art, the following will briefly introduce the drawings required for use in the description of the specific embodiments or the prior art. Obviously, the following drawings are some embodiments of the present invention. For those of ordinary skill in the art, other drawings can be obtained based on these drawings without creative efforts.

[0033] Figure 1 It is a liquid chromatography comparison chart of germinated rice formula granules, stir-fried germinated rice formula granules, and scorched germinated rice formula granules in Example 1 of the present invention;

[0034] Figure 2 It is a liquid chromatography comparison chart of germinated rice formula granules, stir-fried germinated rice formula granules, and scorched germinated rice formula granules in Example 2 of the present invention;

[0035] Figure 3It is the liquid chromatography comparison chart of the germinated rice formula granules, stir-fried germinated rice formula granules, and charred germinated rice formula granules in Example 3 of the present invention;

[0036] Figure 4 It is the liquid chromatography comparison chart of the germinated rice formula granules, stir-fried germinated rice formula granules, and charred germinated rice formula granules in Comparative Example 1;

[0037] Figure 5 It is the liquid chromatography comparison chart of the germinated rice formula granules, stir-fried germinated rice formula granules, and charred germinated rice formula granules in Comparative Example 2. Specific Embodiments

[0038] The following embodiments are provided to better further understand the present invention. It is not limited to the best embodiment, and does not limit the content and protection scope of the present invention. Any product that is the same as or similar to the present invention obtained by anyone under the inspiration of the present invention or by combining the features of the present invention with other prior art features falls within the protection scope of the present invention.

[0039] For those embodiments where specific experimental steps or conditions are not indicated, the operations or conditions of the conventional experimental steps described in the literature in this field can be followed. For the reagents or instruments without indicating the manufacturer, they are all conventional reagent products that can be obtained through commercial purchase.

[0040] Instruments: JY20002 electronic balance (Shanghai Shunyu Hengping Scientific Instrument Co., Ltd.); ME104E electronic balance (Mettler Toledo); XP26 electronic balance (Mettler Toledo Instrument Co., Ltd.); KQ-300DB ultrasonic cleaner (Kunshan Ultrasonic Instrument Co., Ltd.); Waters 2695 high performance liquid chromatograph (UV / Vis Detector detector);

[0041] Chromatographic column: ZORBAX Eclipse Plus C18 (column length 150 mm, inner diameter 2.1 mm, particle size 1.8 μm);

[0042] Test drugs: Germinated rice formula granules (S01, S02, S03, S04, S05, S06, S07, S08, S09, S10, S11, S12, S13, S14, S15, S16, S17);

[0043] Stir-fried germinated rice formula granules (C01, C02, C03, C04, C05, C06, C07, C08, C09, C10, C11, C12, C13, C14, C15, C16, C17);

[0044] Formula granules of stir-fried germinated rice (J01, J02, J03, J04, J05, J06, J07, J08, J09, J10, J11, J12, J13, J14, J15, J16, J17);

[0045] 4-Coumaric acid reference substance (Batch No.: 112037-202102, National Institutes for Food and Drug Control);

[0046] 5-Hydroxymethylfurfural reference substance (Batch No.: 111626-202316, National Institutes for Food and Drug Control).

[0047] Example 1

[0048] A method for detecting and identifying formula granules of germinated rice and its processed products is determined by high performance liquid chromatography (General Principles 0512, Volume IV, Chinese Pharmacopoeia 2020 Edition). The specific process is as follows:

[0049] (1) Solution preparation

[0050] Preparation of test solution: Appropriate amounts of formula granules of germinated rice, stir-fried germinated rice, and stir-fried germinated rice with coke were taken and ground finely. About 0.2 g was taken, accurately weighed, 20 ml of 70% methanol aqueous solution was added, weighed, ultrasonically treated for 30 minutes, taken out, cooled, and the lost weight was made up with 70% methanol aqueous solution. It was shaken well and filtered, and the subsequent filtrate was taken to obtain the test solution.

[0051] Preparation of reference substance solution: Appropriate amounts of 4-coumaric acid reference substance were taken and accurately weighed, and methanol was added to make a 4-coumaric acid reference substance solution containing 10 μg per 1 ml; appropriate amounts of 5-hydroxymethylfurfural reference substance were taken and accurately weighed, and methanol was added to make a 5-hydroxymethylfurfural reference substance solution containing 5 μg per 1 ml.

[0052] (2) Detection by ultra-high performance liquid chromatography

[0053] ZORBAX Eclipse Plus C18 chromatographic column with octadecylsilane chemically bonded silica as the filler (column length is 150 mm, inner diameter is 2.1 mm, particle size is 1.8 μm); acetonitrile is used as mobile phase A, and 0.1% formic acid aqueous solution is used as mobile phase B, and gradient elution is carried out according to the regulations in Table 1 below; the flow rate is 0.35 ml per minute; the column temperature is 35 °C; the detection wavelength is 300 nm. The theoretical plate number calculated by the 4-coumaric acid peak should be not less than 60000.

[0054] 2 μl of the reference substance solution and the test solution were accurately pipetted respectively, injected into the liquid chromatograph for determination to obtain the results. The determination results are shown in Tables 2 to 7 and Figure 1 as shown.

[0055] Table 1 Gradient elution table

[0056]

[0057] Table 2 Results Table of Peak Area Determination of Semen Oryzae Germinatum Formula Granules

[0058]

[0059] Note: " / " indicates not detected.

[0060] Table 3 Results Table of Retention Time Determination of Semen Oryzae Germinatum Formula Granules

[0061]

[0062]

[0063] Note: " / " indicates not detected.

[0064] Table 4 Results Table of Peak Area Determination of Parched Semen Oryzae Germinatum Formula Granules

[0065]

[0066] Table 5 Results Table of Retention Time Determination of Parched Semen Oryzae Germinatum Formula Granules

[0067]

[0068]

[0069] Table 6 Results Table of Peak Area Determination of Carbonized Semen Oryzae Germinatum Formula Granules

[0070]

[0071] Table 7 Results Table of Retention Time Determination of Carbonized Semen Oryzae Germinatum Formula Granules

[0072]

[0073]

[0074] (3) Determination of the Differences among the Three

[0075] From the comparison chart of the liquid chromatograms of Semen Oryzae Germinatum formula granules, parched Semen Oryzae Germinatum formula granules, and carbonized Semen Oryzae Germinatum formula granules measured ( Figure 1 ) it can be seen that peaks 1 and 2 were detected in both parched Semen Oryzae Germinatum formula granules and carbonized Semen Oryzae Germinatum formula granules, while peaks 1 and 2 were not detected in Semen Oryzae Germinatum formula granules. Peak 1 is a peak of an unknown component. Through the determination of the parched Semen Oryzae Germinatum and carbonized Semen Oryzae Germinatum chromatograms, the basis for the positioning of peak 1 is that the relative retention time of peak 1 relative to peak 2 is 0.71 - 0.79, with an average value of 0.75. Therefore, through the presence or absence of peaks 1 and 2, Semen Oryzae Germinatum formula granules can be distinguished from parched Semen Oryzae Germinatum and carbonized Semen Oryzae Germinatum formula granules.

[0076] Both germinated rice and its processed products contain the target component 4-coumaric acid. Peak 2 in the chromatogram is a substance generated by the Maillard reaction between proteins and carbohydrate compounds during the processing, which is 5-hydroxymethylfurfural. The change of this component is positively correlated with the change of color. Therefore, the ratio of peak 3 (4-coumaric acid) to peak 2 (5-hydroxymethylfurfural) can be used as the main difference point between stir-fried germinated rice and stir-fried germinated rice to a charred state. Through the determination of 17 batches of stir-fried germinated rice formula granules (C01 - C17) and 17 batches of stir-fried germinated rice to a charred state formula granule samples (J01 - J17), the ratio of peak 2 / peak 3 of stir-fried germinated rice formula granules is in the range of 0.21 - 0.87, and the ratio of peak 2 / peak 3 of stir-fried germinated rice to a charred state formula granules is in the range of 1.49 - 3.57. Therefore, this ratio range is used as the differentiation point between stir-fried germinated rice formula granules and stir-fried germinated rice to a charred state formula granules.

[0077] Example 2

[0078] This example provides a method for identifying germinated rice and its processed product formula granules. The difference from Example 1 is that the detection wavelength is 295 nm, and mobile phase B is an aqueous formic acid solution with a volume percentage of 0.08%. Other conditions are the same as those in Example 1.

[0079] The test results are as Figure 2 and the following table shows:

[0080] Table 8 Determination results of peak areas of germinated rice formula granules

[0081]

[0082] Note: " / " indicates not detected.

[0083] Table 9 Determination results of retention times of germinated rice formula granules

[0084]

[0085] Note: " / " indicates not detected.

[0086] Table 10 Determination results of peak areas of stir-fried germinated rice formula granules

[0087]

[0088] Table 11 Determination results of retention times of stir-fried germinated rice formula granules

[0089]

[0090]

[0091] Table 12 Determination results of peak areas of stir-fried germinated rice to a charred state formula granules

[0092]

[0093] Table 13 Determination Results of Retention Time of Prepared Granules of Stir-Fried Germinated Rice

[0094]

[0095]

[0096] The above detection results are basically the same as those in Example 1, which proves that under the conditions of detection wavelength: 295 nm and mobile phase B: 0.08% formic acid aqueous solution, it has basically the same effect as the conditions of detection wavelength: 300 nm and mobile phase B: 0.10% formic acid aqueous solution. The parameter conditions of this example can also be effectively used for the detection and identification of prepared granules of germinated rice and its processed products.

[0097] Example 3

[0098] This example provides a method for identifying prepared granules of germinated rice and its processed products. The difference from Example 1 is that the detection wavelength is 305 nm, the mobile phase B is an aqueous solution of formic acid with a volume percentage of 0.12%, and other conditions are the same as those in Example 1.

[0099] The detection results are as Figure 3 shown in the following table:

[0100] Table 14 Determination Results of Peak Area of Prepared Granules of Germinated Rice

[0101]

[0102] Note: " / " indicates not detected.

[0103] Table 15 Determination Results of Retention Time of Prepared Granules of Germinated Rice

[0104]

[0105] Note: " / " indicates not detected.

[0106] Table 16 Determination Results of Peak Area of Prepared Granules of Stir-Fried Germinated Rice

[0107]

[0108] Table 17 Determination Results of Retention Time of Prepared Granules of Stir-Fried Germinated Rice

[0109]

[0110] Table 18 Determination Results of Peak Area of Prepared Granules of Stir-Fried Germinated Rice to Dark Brown

[0111]

[0112] Table 19 Determination Results of Retention Time of Prepared Granules of Stir-Fried Germinated Rice to Dark Brown

[0113]

[0114] The above detection results are basically the same as those in Example 1, proving that under the conditions of detection wavelength: 305 nm and mobile phase B: 0.12% formic acid aqueous solution, it has basically the same effect as the detection wavelength: 300 nm and mobile phase B: 0.10% formic acid aqueous solution. The parameter conditions of this example can also be effectively used for the detection and identification of germinated rice and its processed formula granules.

[0115] Comparative Example 1

[0116] This comparative example provides a method for identifying germinated rice and its processed formula granules. The chromatographic conditions are as follows: using octadecylsilane-bonded silica gel as the filler (column length 100 mm, inner diameter 2.1 mm, particle size 1.7 μm); using acetonitrile as mobile phase A and 0.1% formic acid aqueous solution as the mobile phase, and performing gradient elution according to the regulations in Table 20 below; the flow rate is 0.3 ml per minute; the column temperature is 25°C; the detection wavelength is 297 nm. Other conditions are the same as those in Example 1.

[0117] Table 20 Gradient Elution Table

[0118]

[0119]

[0120] Determination was carried out according to the chromatographic conditions of Comparative Example 1, and the obtained chromatogram is as Figure 4 shown, Figure 4 in which the corresponding chromatographic peak of 4-coumaric acid cannot be found, so the three cannot be distinguished and identified.

[0121] Comparative Example 2

[0122] This comparative example provides a method for identifying germinated rice and its processed formula granules. The chromatographic conditions are as follows: using octadecylsilane-bonded silica gel as the filler (column length 250 mm, inner diameter 4.6 mm, particle size 5 μm); using acetonitrile as mobile phase A and 0.5% acetic acid aqueous solution as mobile phase B, and performing gradient elution according to the regulations in Table 21 below; the flow rate is 0.8 ml per minute, the column temperature is 30°C; the detection wavelength is 300 nm. Other conditions are the same as those in Example 1.

[0123] Table 21 Gradient Elution Table

[0124]

[0125] Determination was carried out according to the chromatographic conditions in Comparative Example 2, and the results are as Figure 5 shown, and through Figure 5Display: The peaks before 5-hydroxymethylfurfural could not achieve chromatographic peak separation, and the 5-hydroxymethylfurfural peak was not completely separated from other peaks, making it impossible to accurately perform chromatographic peak integration. Therefore, this method could not distinguish among the three substances.

[0126] Obviously, the above embodiments are merely examples given for clear illustration and are not limitations on the implementation manners. For those of ordinary skill in the art, other different forms of changes or modifications can be made based on the above description. It is not necessary and impossible to enumerate all implementation manners here. And the obvious changes or modifications derived therefrom are still within the protection scope of the present invention.

Claims

1. A method for identifying germinated rice and its prepared formula granules, characterized in that, It includes the following steps: Obtain the test solution of Fructus Oryzae Germinatus and its processed products; Detect the test solution by ultra-high performance liquid chromatography to obtain a liquid chromatography diagram containing characteristic peaks corresponding to 4-coumaric acid and 5-hydroxymethylfurfural; The formula granules of Fructus Oryzae Germinatus and its processed products include: stir-fried Fructus Oryzae Germinatus formula granules, scorched Fructus Oryzae Germinatus formula granules, and Fructus Oryzae Germinatus formula granules; The characteristic peak corresponding to 5-hydroxymethylfurfural is missing in the liquid chromatography diagram of Fructus Oryzae Germinatus formula granules; In the liquid chromatography diagram of stir-fried Fructus Oryzae Germinatus formula granules, the peak area ratio of 5-hydroxymethylfurfural to 4-coumaric acid is within the range of 0.21 - 0.87; In the liquid chromatography diagram of scorched Fructus Oryzae Germinatus formula granules, the peak area ratio of 5-hydroxymethylfurfural to 4-coumaric acid is within the range of 1.49 - 3.57; 2. The identification method according to claim 1, wherein In the liquid chromatography diagrams of stir-fried Fructus Oryzae Germinatus formula granules and scorched Fructus Oryzae Germinatus formula granules, there is also a characteristic peak peak 1 with a relative retention time of 0.71 - 0.79 relative to the characteristic peak corresponding to 5-hydroxymethylfurfural, and peak 1 is missing in the liquid chromatography diagram of Fructus Oryzae Germinatus formula granules; 3. A detection method for germinated rice and its processed product formula granules used in the identification method according to claim 1 or 2, characterized in that, The detection method is ultra-high performance liquid chromatography, and the detection conditions of the ultra-high performance liquid chromatography include: octadecylsilane-bonded silica gel as the filler, detection wavelength of 295 - 305 nm, acetonitrile as mobile phase A, and formic acid aqueous solution with a volume percentage of 0.08 - 0.12% as mobile phase B; Perform elution according to the following gradient program: The liquid chromatography diagram obtained by the ultra-high performance liquid chromatography detection includes a characteristic peak peak 3 corresponding to 4-coumaric acid, a characteristic peak peak 2 corresponding to 5-hydroxymethylfurfural, and a characteristic peak peak 1 with a relative retention time of 0.71 - 0.79 relative to peak 2; 4. The detection method according to claim 3, wherein Among the detection conditions of the ultra-high performance liquid chromatography, the column length of the chromatographic column is 150 mm, the inner diameter is 2.1 mm, and the particle size is 1.8 μm; 5. The detection method according to claim 3 or 4, characterized in that, Among the detection conditions of the ultra-high performance liquid chromatography, the flow rate is 0.33 - 0.37 ml / min, preferably 0.35 ml / min; 6. The detection method according to any one of claims 3 to 5, characterized in that, Among the detection conditions of the ultra-high performance liquid chromatography, the column temperature is 32 - 38 °C, preferably 35 °C; 7. The detection method according to any one of claims 3 to 6, characterized in that Among the detection conditions of the ultra-high performance liquid chromatography, the injection volume is 1 - 3 μl, preferably 2 μl; 8. The detection method according to any one of claims 3 to 7, characterized in that, Among the detection conditions of the ultra-high performance liquid chromatography, the number of theoretical plates calculated based on the 4-coumaric acid peak should not be less than 60000; 9. The detection method according to any one of claims 3 to 8, characterized in that The preparation process of the test solution is: take the test sample, grind it finely, accurately weigh it, add the solvent, weigh the weight, perform extraction treatment, take it out, let it cool, weigh the weight again, make up the lost weight with the corresponding solvent, shake well, filter, and take the subsequent filtrate to obtain the test solution; 10. The detection method according to claim 9, characterized in that, The extraction treatment is ultrasonic treatment; And / or, the duration of the extraction treatment is 20 - 40 min, preferably 30 min; And / or, the solvent is a methanol aqueous solution with a volume percentage of 60 - 80%, preferably a methanol aqueous solution with a volume percentage of 70%; And / or, relative to 10 - 30 ml of the solvent, the addition amount of the test sample is 0.1 - 0.3 g, preferably 0.2 g of the test sample is added corresponding to 20 ml of the solvent.

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