Sample suction and counting control system and method

Abstract

The invention relates to the technical field of medical instruments, in particular to a sample suction and counting control system and method.The method includes the steps that a metal needle is immersed in a sample in a sample tube, an injector module is opened to suck the sample, a DIL outputs liquid into a gas-liquid mixing device, the sample tube leaves the metal needle, then the mixed liquid on the outer wall of the DIL and the outer wall of the metal needle is pumped to the outside, and the gas-liquid mixing device is started; the metal needle adds the blood sample into the sample mixing device for mixing reaction; the residual sample and liquid in the metal needle are sucked into a storage pool device, the injector module moves downwards by a certain motor step number, and the step number is counted; the suction device sucks the sample in the sample mixing device, part of the mixed liquid of the sample and the liquid is flushed into the storage pool device, and meanwhile, the injector module moves upwards to beat the sample flow into the counting device; after the injector module completes counting steps, the counting device is flushed, the system reaches the initial state, and the method achieves the purposes of reducing samples, increasing the speed and reducing the single test cost by sharing one injection unit.

Description

Technical Field

[0001] The present invention relates to the technical field of medical devices, and in particular to a sampling and counting control system and method. Background Art

[0002] Blood cell analysis, as an indispensable part of clinical diagnosis, is not only one of the three routine tests, but also plays a crucial role in diagnosing blood-related diseases. However, blood analyzer technologies with extremely high precision, strong specificity (such as high recognition ability for abnormal cells), and the ability to measure multiple parameters simultaneously usually adopt micro sheath flow impedance technology in cooperation with a high-precision syringe sampling unit and a sampling unit, and are realized through a complex logic control system.

[0003] The equipment of the control system is not only fast, but also can provide key diagnostic data, which is crucial for improving the accuracy and efficiency of diagnosis. However, the equipment of the traditional control system usually has a high single test cost, complex instrument maintenance, and a high price. Summary of the Invention

[0004] The purpose of the present invention is to provide a sampling and counting control system and method, aiming to solve the problems that the equipment of the traditional control system usually has a high single test cost and a high price.

[0005] To achieve the above purpose, in the first aspect, the present invention provides a sampling and counting control method, including the following steps;

[0006] Immerse the sample in the sample tube in the metal needle, open the syringe module to suck a fixed amount of sample, the third DIL outputs liquid into the gas-liquid mixing device, remove the sample tube from the metal needle, then suck the mixed liquid on the outer wall of the third DIL and the metal needle to the outside, and add the blood sample to the sample mixing device through the metal needle for mixing reaction;

[0007] Suck the remaining sample and liquid in the metal needle into the third storage pool device, and the fourth DIL outputs liquid. The syringe module moves down a certain number of motor steps, count the number of motor steps, and the syringe module stops running;

[0008] The sucking device acts to suck the sample in the sample mixing device, and the sucking device acts again to wash part of the mixed liquid of the sample and liquid into the second storage pool device. The first DIL outputs liquid, and at the same time the syringe module moves upward to pump the sample flow into the counting device;

[0009] The sample is pushed by the syringe module, and the counting device outputs different signals generated by different samples. After the syringe module runs through the counted number of steps, the counting device is washed, and the system reaches the initial state.

[0010] Among them, the specific method of submerging the sample in the sample tube into the metal needle, opening the syringe module to aspirate a quantitative sample, the third DIL outputting liquid into the gas-liquid mixing device, removing the sample tube from the metal needle, and then pumping the mixed liquid on the outer wall of the third DIL and the metal needle to the outside, and adding the blood sample to the sample mixing device through the metal needle for mixing reaction:

[0011] Submerge the sample in the sample tube into the metal needle, and start the waste liquid pump to output a high-speed reverse air flow;

[0012] The solenoid valves S11 and S10 are opened simultaneously to operate the syringe module, and it moves downward to aspirate a quantitative sample;

[0013] The third DIL outputs liquid into the gas-liquid mixing device, and at the same time, the sample tube is removed from the metal needle;

[0014] The metal needle moves upward, and the waste liquid pump pumps the mixed liquid on the outer wall of the third DIL and the metal needle to the outside;

[0015] Add the blood sample to the sample mixing device through the metal needle for mixing reaction.

[0016] Among them, the specific method of inhaling the remaining sample and liquid in the metal needle into the third storage pool device, the fourth DIL outputting liquid, the syringe module moving down a certain number of motor steps, counting the number of motor steps, and the syringe module stopping operating:

[0017] Open the solenoid valve S13, cooperate with the solenoid valve S10, the third DIL outputs, and inhale the remaining sample and liquid in the metal needle into the third storage pool device;

[0018] Close the solenoid valves S13 and S10, open the solenoid valve S12, and the fourth DIL outputs liquid;

[0019] The syringe module moves down a certain number of motor steps and reaches the requirement for counting the number of motor steps;

[0020] The syringe module stops operating, and the solenoid valve S12 is closed.

[0021] Among them, the specific method of the aspiration device operating to aspirate the sample in the sample mixing device, the aspiration device operating again to wash part of the mixed liquid of the sample and liquid into the second storage pool device, the first DIL outputting liquid, and at the same time the syringe module moving upward to pump the sample flow into the counting device:

[0022] The solenoid valves S6, S7, and S9 are opened, and the suction device operates to suck the sample in the sample mixing device between the solenoid valves S6 and S7.

[0023] The solenoid valves S6 and S7 are closed, and the suction device operates to flush a part of the mixture of the sample and the liquid into the second storage tank device.

[0024] The solenoid valves S11, S1, and S3 are opened, the first DIL outputs liquid, and at the same time, the syringe module moves upward to inject the sample flow in the solenoid valves S6 and S7 into the counting device.

[0025] In a second aspect, the present invention further provides a sample suction and counting control system, which is applied to the sample suction and counting control method described in the first aspect above, and includes a syringe module, a first DIL, a second DIL, a third DIL, a fourth DIL, a metal needle, a gas-liquid mixing device, a first storage tank device, a second storage tank device, a third storage tank device, a counting device, a waste liquid pump, a sample mixing device, a suction device, solenoid valves S1, S2, S3, S4, S5, S6, S7, S8, S9, S10, S11, S12, and S13. The first DIL, the solenoid valve S1, the solenoid valve S2, the solenoid valve S3, the solenoid valve S6, the solenoid valve S11, the syringe module, the solenoid valve S12, and the fourth DIL are connected in sequence. The gas-liquid mixing device is respectively connected to the solenoid valve S5, the solenoid valve S1, the first storage tank device, the solenoid valve S8, the solenoid valve S6, and the solenoid valve S7. The second DIL is connected to the solenoid valve S5. The suction device is respectively connected to the solenoid valve S7, the second storage tank device, and the solenoid valve S9. The gas-liquid mixing device is respectively connected to the waste liquid pump, the third DIL, and the metal needle. The solenoid valve S10 is respectively connected to the metal needle, the solenoid valve S13, and the solenoid valve S11. The third storage tank device is connected to the solenoid valve S13. The sample mixing device is connected to the solenoid valve S6.

[0026] A sampling and counting control method of the present invention submerges the sample in the sample tube into the metal needle, opens the syringe module to aspirate a fixed amount of the sample, the DIL outputs liquid to the gas-liquid mixing device, separates the sample tube from the metal needle, then pumps the mixed liquid on the outer wall of the DIL and the metal needle to the outside, adds the blood sample to the sample mixing device through the metal needle for mixing reaction; sucks the remaining sample and liquid in the metal needle into the first storage pool device, and the DIL outputs liquid, the syringe module moves down a certain number of motor steps, counts the number of motor steps, and the syringe module stops running; the sucking device acts to suck the sample in the sample mixing device, and the sucking device acts again to wash part of the mixed liquid of the sample and liquid into the second storage pool device, the DIL outputs liquid, and at the same time the syringe module moves upward to pump the sample flow into the counting device; the sample is pushed by the syringe module, and the counting device outputs different signals generated by different samples. After the syringe module runs through the counting steps, the counting device is washed, and the system reaches the initial state. This method uses the syringe module to cooperate logically with multiple solenoid valves to quantitatively aspirate the sample in the tube with the metal needle, add the sample to the sample mixing device for quantitative reaction, and at the same time the gas-liquid mixing device cooperates with the DIL to wash the outer wall of the sample mixing device, and outputs a high-speed reverse air flow through the waste liquid pump (P-1) to suck the mixed liquid into the W2 liquid storage pool; starts the sucking device to suck the mixture into the counting device, and uses the syringe module to push the sample to the other end in the counting device, so as to obtain more counting parameters. On the premise of ensuring more performance parameter indicators, test speed and low cost of the instrument, by sharing a single injection unit, the cleaning of the pipeline is reduced, the sample is reduced, the speed is increased, and the single test cost is reduced. It solves the problem that the equipment of the traditional control system usually has a high cost for a single test. Description of the Drawings

[0027] In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the drawings required for use in the description of the embodiments or the prior art. Obviously, the following drawings are only some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings.

[0028] Figure 1 It is a flowchart of a sampling and counting control method provided by the present invention.

[0029] Figure 2It is a flowchart showing that the sample in the sample tube is immersed in a metal needle, the syringe module is opened to aspirate a fixed amount of the sample, the DIL outputs liquid into the gas-liquid mixing device, the sample tube is removed from the metal needle, then the mixture on the outer wall of the DIL and the metal needle is pumped out, and the blood sample is added to the sample mixing device through the metal needle for mixing reaction.

[0030] Figure 3 It is a flowchart showing that the remaining sample and liquid in the metal needle are aspirated into the storage pool device, the DIL outputs liquid, the syringe module moves down a certain number of motor steps, the number of motor steps is counted, and the syringe module stops running.

[0031] Figure 4 It is a flowchart showing that the aspiration device operates to aspirate the sample in the sample mixing device, the aspiration device operates again to flush a part of the mixture of the sample and liquid into the storage pool device, the DIL outputs liquid, and at the same time the syringe module moves upward to pump the sample flow into the counting device.

[0032] Figure 5 It is a schematic connection diagram of a sample aspiration and counting control system provided by the present invention.

[0033] In the figure: 1 - syringe module, 2 - first DIL, 3 - second DIL, 4 - third DIL, 5 - fourth DIL, 6 - metal needle, 7 - gas-liquid mixing device, 8 - first storage pool device, 9 - second storage pool device, 10 - third storage pool device, 11 - counting device, 12 - waste liquid pump, 13 - sample mixing device, 14 - aspiration device, 15 - solenoid valve S1, 16 - solenoid valve S2, 17 - solenoid valve S3, 18 - solenoid valve S4, 19 - solenoid valve S5, 20 - solenoid valve S6, 21 - solenoid valve S7, 22 - solenoid valve S8, 23 - solenoid valve S9, 24 - solenoid valve S10, 25 - solenoid valve S11, 26 - solenoid valve S12, 27 - solenoid valve S13. Detailed implementation manners

[0034] The embodiments of the present invention will be described in detail below. The examples of the embodiments are shown in the accompanying drawings, where the same or similar reference numerals denote the same or similar elements or elements having the same or similar functions from beginning to end. The embodiments described below with reference to the accompanying drawings are exemplary and are intended to explain the present invention, and should not be construed as limiting the present invention.

[0035] Please refer to Figures 1 to 5 , in the first aspect, the present invention provides a sample aspiration and counting control method, including the following steps;

[0036] S1 Immerse the sample in the sample tube in the metal needle (6), open the syringe module (1) to aspirate a quantified sample, the third DIL (4) outputs the liquid into the gas-liquid mixing device (7), remove the sample tube from the metal needle (6), then draw the mixed liquid on the outer wall of the third DIL (4) and the metal needle (6) to the outside, and add the blood sample to the sample mixing device (13) through the metal needle (6) for mixing reaction;

[0037] Specific method:

[0038] S11 Immerse the sample in the sample tube in the metal needle (6), start the waste liquid pump (12) to output a high-speed reverse air flow;

[0039] S12 The solenoid valve S11 (25) and the solenoid valve S10 (24) simultaneously open the syringe module (1), and move downward to aspirate a quantified sample;

[0040] S13 The third DIL (4) outputs the liquid into the gas-liquid mixing device (7), and at the same time remove the sample tube from the metal needle (6);

[0041] S14 The metal needle (6) moves upward, and the waste liquid pump (12) draws the mixed liquid on the outer wall of the third DIL (4) and the metal needle (6) to the outside;

[0042] S15 Add the blood sample to the sample mixing device (13) through the metal needle (6) for mixing reaction.

[0043] S2 Inhale the remaining sample and liquid in the metal needle (6) into the third storage tank device (10), and the fourth DIL (5) outputs the liquid. The syringe module (1) moves down a certain number of motor steps, count the number of motor steps, and the syringe module (1) stops running;

[0044] Specific method:

[0045] S21 Open the solenoid valve S13 (27), cooperate with the solenoid valve S10 (24), the third DIL (4) outputs, and inhale the remaining sample and liquid in the metal needle (6) into the third storage tank device (10);

[0046] S22 Close the solenoid valve S13 (27) and the solenoid valve S10 (24), open the solenoid valve S12 (26), and the fourth DIL (5) outputs the liquid;

[0047] S23 The syringe module (1) moves down a certain number of motor steps and reaches the requirement for counting the number of motor steps used;

[0048] S24 The syringe module (1) stops running, and close the solenoid valve S12 (26).

[0049] The S3 suction device (14) operates to suck the sample in the sample mixing device (13). Then the suction device (14) operates again to flush a part of the mixed liquid of the sample and the liquid into the second storage tank device (9). The first DIL (2) outputs the liquid, and at the same time, the syringe module (1) moves upward to inject the sample flow into the counting device (11).

[0050] Specific method:

[0051] S31 The solenoid valves S6 (16), S7 (17) and S9 (19) are opened, and the suction device (14) operates to suck the sample in the sample mixing device (13) to between the solenoid valves S6 (16) and S7 (17).

[0052] S32 The solenoid valves S6 (16) and S7 (17) are closed, and the suction device (14) operates to flush a part of the mixed liquid of the sample and the liquid into the second storage tank device (9).

[0053] S33 The solenoid valves S11 (25), S1 (15) and S3 (17) are opened. The first DIL (2) outputs the liquid, and at the same time, the syringe module (1) moves upward to inject the sample flow in the solenoid valves S6 (16) and S7 (17) into the counting device (11).

[0054] S4 Through the push of the syringe module (1), the counting device (11) outputs different signals generated by different samples. After the syringe module (1) completes the counting steps, the counting device (11) is flushed, and the system returns to the initial state.

[0055] In the embodiment of the present invention, through the push of the syringe module (1), the counting device (11) outputs different signals generated by different samples. After the syringe module (1) completes the counting steps, the solenoid valves S12 (26), S7 (17), S8 (18), S4 (20), S2 (21) and S5 (22) are opened to flush the counting device (11) and the sample pipeline between the solenoid valves S6 (16) and S7 (17), so that the system returns to the initial state.

[0056] Please refer to the figure. In a second aspect, the present invention further provides a sampling and counting control system, which is applied to the sampling and counting control method described in the first aspect above. The system includes a syringe module (1), a first DIL (2), a second DIL (3), a third DIL (4), a fourth DIL (5), a metal needle (6), a gas-liquid mixing device (7), a first storage tank device (8), a second storage tank device (9), a third storage tank device (10), a counting device (11), a waste liquid pump (12), a sample mixing device (13), a suction device (14), a solenoid valve S1 (15), a solenoid valve S2 (21), a solenoid valve S3 (17), a solenoid valve S4 (20), a solenoid valve S5 (22), a solenoid valve S6 (16), a solenoid valve S7 (17), a solenoid valve S8 (18), a solenoid valve S9 (19), a solenoid valve S10 (24), a solenoid valve S11 (25), a solenoid valve S12 (26), and a solenoid valve S13 (27). The first DIL (2), the solenoid valve S1 (15), the solenoid valve S2 (21), the solenoid valve S3 (17), the solenoid valve S6 (16), the solenoid valve S11 (25), the syringe module (1), the solenoid valve S12 (26), and the fourth DIL (5) are connected in sequence. The gas-liquid mixing device (7) is respectively connected to the solenoid valve S5 (22), the solenoid valve S1 (15), the first storage tank device (8), the solenoid valve S8 (18), the solenoid valve S6 (16), and the solenoid valve S7 (17). The second DIL (3) is connected to the solenoid valve S5 (22). The suction device (14) is respectively connected to the solenoid valve S7 (17), the second storage tank device (9), and the solenoid valve S9 (19). The gas-liquid mixing device (7) is respectively connected to the waste liquid pump (12), the third DIL (4), and the metal needle (6). The solenoid valve S10 (24) is respectively connected to the metal needle (6), the solenoid valve S13 (27), and the solenoid valve S11 (25). The third storage tank device (10) is connected to the solenoid valve S13 (27). The sample mixing device (13) is connected to the solenoid valve S6 (16).

[0057] In the embodiment of the present invention, the syringe module (1) is used to provide the aspiration of samples and the propulsion and quantification of the mixture; the first DIL (2), the second DIL (3), the third DIL (4) and the fourth DIL (5) are used to provide the flushing of the system and generate laminar flow for certain special requirements; the metal needle (6) is used to aspirate the samples in the sample tube; the gas-liquid mixing device (7) is used to perform gas-liquid mixing on the mixture on the outer wall of the metal needle (6); the first storage pool device (8), the second storage pool device (9) and the third storage pool device (10) are used to store the liquids generated in each stage of the system; the counting device (11) is used to count the samples and obtain corresponding multiple parameters; the waste liquid pump (12) is used to output a high-speed reverse air flow to aspirate the gas-liquid mixture of the DIL and the gas-liquid mixing device (7) into the external W2 liquid storage pool; the sample mixing device (13) is used for sample mixing and counting; the aspiration device (14) is used to aspirate the mixture in the sample mixing device (13) after the reaction into the counting device (11); the solenoid valves S1 (15), solenoid valve S2 (21), solenoid valve S3 (17), solenoid valve S4 (20), solenoid valve S5 (22), solenoid valve S6 (16), solenoid valve S7 (17), solenoid valve S8 (18), solenoid valve S9 (19), solenoid valve S10 (24), solenoid valve S11 (25), solenoid valve S12 (26) and solenoid valve S13 (27) are used in cooperation to control the on-off of the system pipeline and the liquid flow logic.

[0058] The above-disclosed is only a preferred embodiment of a sample aspiration and counting control system and method of the present invention. Of course, the scope of rights of the present invention cannot be limited thereby. Those of ordinary skill in the art can understand all or part of the processes of implementing the above embodiments, and the equivalent changes made according to the claims of the present invention still fall within the scope covered by the invention.

Claims

1. A sampling and counting control method, characterized in that, Including the following steps; Immerse the sample in the sample tube in the metal needle, open the syringe module to aspirate a quantitative sample, the third DIL outputs liquid into the gas-liquid mixing device, remove the sample tube from the metal needle, then pump the mixed liquid on the outer wall of the third DIL and the metal needle to the outside, and add the blood sample to the sample mixing device through the metal needle for mixing reaction; Aspirate the remaining sample and liquid in the metal needle into the third storage tank device, and the fourth DIL outputs liquid. The syringe module moves down a certain number of motor steps, count the number of motor steps, and the syringe module stops running; The aspiration device operates to aspirate the sample in the sample mixing device. The aspiration device operates again to flush part of the mixed liquid of the sample and liquid into the second storage tank device. The first DIL outputs liquid, and at the same time the syringe module moves upward to pump the sample stream into the counting device; The sample is pushed by the syringe module, and the counting device outputs different signals generated by different samples. After the syringe module runs through the counted number of steps, the counting device is flushed, and the system reaches the initial state.

2. The sampling and counting control method according to claim 1, It is characterized in that; The specific method of immersing the sample in the sample tube in the metal needle, opening the syringe module to aspirate a quantitative sample, the third DIL outputting liquid into the gas-liquid mixing device, removing the sample tube from the metal needle, then pumping the mixed liquid on the outer wall of the third DIL and the metal needle to the outside, and adding the blood sample to the sample mixing device through the metal needle for mixing reaction: Immerse the sample in the sample tube in the metal needle, and start the waste liquid pump to output a high-speed reverse air flow; Simultaneously open the syringe module with solenoid valve S11 and solenoid valve S10, and move downward to aspirate a quantitative sample; The third DIL outputs liquid into the gas-liquid mixing device, and at the same time remove the sample tube from the metal needle; The metal needle moves upward, and the waste liquid pump pumps the mixed liquid on the outer wall of the third DIL and the metal needle to the outside; Add the blood sample to the sample mixing device through the metal needle for mixing reaction.

3. The sample aspiration and counting control method according to claim 1, characterized in that; The specific method of aspirating the remaining sample and liquid in the metal needle into the third storage tank device, the fourth DIL outputting liquid, the syringe module moving down a certain number of motor steps, counting the number of motor steps, and the syringe module stopping running: Open solenoid valve S13, cooperate with solenoid valve S10, the third DIL outputs, and aspirate the remaining sample and liquid in the metal needle into the third storage tank device; Close solenoid valve S13 and solenoid valve S10, open solenoid valve S12, and the fourth DIL outputs liquid; The syringe module moves down a certain number of motor steps and reaches the requirement for counting the number of motor steps used; The syringe module stops running, and close solenoid valve S12.

4. The sampling and counting control method according to claim 1, wherein ； The sucking device operates to suck the sample in the sample mixing device, and then the sucking device operates to flush a part of the mixture of the sample and the liquid into the second storage tank device. The first DIL outputs liquid, and at the same time, the syringe module moves upward to inject the sample flow into the counting device in the following specific manner: The solenoid valves S6, S7, and S9 are opened, and the sucking device operates to suck the sample in the sample mixing device to the position between the solenoid valves S6 and S7; The solenoid valves S6 and S7 are closed, and the sucking device operates to flush a part of the mixture of the sample and the liquid into the second storage tank device; The solenoid valves S11, S1, and S3 are opened, the first DIL outputs liquid, and at the same time, the syringe module moves upward to inject the sample flow in the solenoid valves S6 and S7 into the counting device.

5. A sampling and counting control system, applied to the sampling and counting control method according to any one of claims 1-4, characterized in that ； It includes a syringe module, a first DIL, a second DIL, a third DIL, a fourth DIL, a metal needle, a gas-liquid mixing device, a first storage tank device, a second storage tank device, a third storage tank device, a counting device, a waste liquid pump, a sample mixing device, a sucking device, solenoid valves S1, S2, S3, S4, S5, S6, S7, S8, S9, S10, S11, S12, and S13. The first DIL, the solenoid valve S1, the solenoid valve S2, the solenoid valve S3, the solenoid valve S6, the solenoid valve S11, the syringe module, the solenoid valve S12, and the fourth DIL are connected in sequence. The gas-liquid mixing device is respectively connected to the solenoid valve S5, the solenoid valve S1, the first storage tank device, the solenoid valve S8, the solenoid valve S6, and the solenoid valve S7. The second DIL is connected to the solenoid valve S5. The sucking device is respectively connected to the solenoid valve S7, the second storage tank device, and the solenoid valve S9. The gas-liquid mixing device is respectively connected to the waste liquid pump, the third DIL, and the metal needle. The solenoid valve S10 is respectively connected to the metal needle, the solenoid valve S13, and the solenoid valve S11. The third storage tank device is connected to the solenoid valve S13. The sample mixing device is connected to the solenoid valve S6.

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