Strontium-containing growth regulator and application thereof in improving stress resistance of plants
Through the growth regulator containing strontium, the problem of poor stress resistance in low-temperature environments is solved, the seed germination rate and seedling growth are improved, and the plants' tolerance to low-temperatures is enhanced.
Patent Information
- Application Number
- CN202510497181.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-04-21
- Publication Date
- 2025-07-22
AI Technical Summary
The prior art has failed to effectively improve the stress resistance of plants in low temperature environments, especially seed germination rate and seedling growth and development.
A strontium-containing growth regulator is used, consisting of jasmonic acid, oxolactone, pepper leaf extract, microbial extract, choline chloride, potassium chlorinate, betaine and white rake tart polysaccharide, and the anti-low-temperature stress performance of plants is improved by soaking seeds or irrigation treatment.
It significantly improves the germination rate of plant seeds in low-temperature environments and the growth performance of seedlings, alleviates the inhibitory effect of low-temperature stress on plants, and enhances the tolerance of plants to low-temperature.
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Abstract
Description
Technical Field
[0001] The present invention belongs to the technical field of plant growth regulators, and relates to a strontium-containing growth regulator and its application in improving plant stress resistance. Background Art
[0002] Strontium is an important trace element and is widely used in fields such as medicine, petroleum, and chemical industry. In recent years, with the in-depth research on plant biology, strontium has gradually attracted people's attention as an important influencing factor in aspects such as plant growth and development, nutrient absorption, and immune system.
[0003] Research shows that strontium can promote plant growth, promote the arrival of the flowering period, and improve fruit quality. However, its excessive content will inhibit plant growth, limit the flowering period and reproductive function, etc., affecting plant growth and development. Strontium can affect the efficiency and rate of plants absorbing other nutrient elements. Existing experimental results show that strontium can improve the absorption rate of basic elements such as phosphorus and potassium by plants, and promote the normal progress of photosynthesis. However, when the content of strontium exceeds a specific threshold, it will inhibit the efficiency of plants absorbing other important elements, resulting in nutrient imbalance. Strontium can improve the disease resistance of plants by improving the antioxidant capacity in the plant body. It is found that an appropriate amount of strontium can promote the photosynthesis of plant leaves, improve the activity of antioxidant enzymes, reduce the activity of peroxidase, and improve the defense of plants against pathogenic microorganisms.
[0004] The application of strontium in the agricultural field cannot be underestimated, but there is no relevant report on strontium in improving plant stress resistance. Summary of the Invention
[0005] The main object of the present invention is to provide a strontium-containing growth regulator. The shown growth regulator can significantly improve the stress resistance of plants, especially the performance of resisting low-temperature stress, and can significantly improve the germination rate of plant seeds in a low-temperature environment and promote the growth and development of seedlings in a low-temperature environment.
[0006] The present invention adopts the following technical solutions to achieve the above object:
[0007] A strontium-containing growth regulator mainly consists of component A and component B. Among them, component A contains 0.3 - 0.5 parts by weight of jasmonic acid, 0.2 - 0.25 parts by weight of ligustilide, 1 - 1.5 parts by weight of pricklyash leaf extract, and component B contains 10 - 12 parts by weight of microbial extract, 0.1 - 0.3 parts by weight of choline chloride, 15 - 20 parts by weight of potassium humate, 1 - 1.5 parts by weight of betaine, 5 - 8 parts by weight of polysaccharide of irpex lacteus, 100 parts by weight of water, and 10 - 15 parts by weight of surfactant.
[0008] The preparation method of the growth regulator is as follows:
[0009] Dissolve jasmonic acid, ligustilide, and the extract of Zanthoxylum bungeanum leaves in ethanol to obtain Component A; dissolve the microbial extract, choline chloride, potassium fulvate, betaine, and polysaccharide of Irpex lacteus in water, and then add a surfactant and mix evenly to obtain Component B; mix Component A and Component B evenly to obtain the growth regulator.
[0010] Furthermore, the preparation method of the extract of Zanthoxylum bungeanum leaves in Component A of the growth regulator is as follows:
[0011] Step A: After drying the Zanthoxylum bungeanum leaves, remove the volatile substances by steam distillation, and dry the distilled Zanthoxylum bungeanum leaves.
[0012] Step B: Extract the distilled Zanthoxylum bungeanum leaves with a mixed solution of n-butanol and ethyl acetate, filter, concentrate the filtrate under reduced pressure to remove the extraction solvent, and dry to obtain the extract of Zanthoxylum bungeanum leaves.
[0013] Furthermore, the volume ratio of n-butanol to ethyl acetate in Step B is 10:2.
[0014] Furthermore, the preparation method of the microbial extract in Component B of the growth regulator is as follows:
[0015] Step 1: Inoculate the seed liquid of Paecilomyces hepiali into a medium containing 20 g / L of sucrose, 5 g / L of peptone, 5 g / L of the extract of Enteromorpha prolifera, 0.3 g / L of magnesium sulfate, and 0.8 g / L of potassium dihydrogen phosphate, and oscillate and ferment at 25°C and 500 rpm for 60 h to obtain the primary fermentation broth.
[0016] Step 2: Add strontium nitrate, polysaccharide of Unio douglasiae, and salicylic acid to the primary fermentation broth, and then continue to oscillate and ferment at 25°C and 500 rpm for 6 h to obtain the final fermentation broth.
[0017] Step 3: Add cellulase, snailase, and lysozyme to the final fermentation broth, enzymatically hydrolyze at 35°C for 1 h, heat to boiling to inactivate the enzyme, centrifuge, take the supernatant, and dry to obtain the microbial extract.
[0018] Furthermore, the OD value of the seed liquid of Paecilomyces hepiali in Step 1 is 0.6.
[0019] Furthermore, the inoculation amount of the seed liquid of Paecilomyces hepiali in Step 1 is 10%.
[0020] Furthermore, the preparation method of the extract of Enteromorpha prolifera in Step 1 is as follows:
[0021] Grind Enteromorpha prolifera with water to make a homogenate, add cellulase and mix evenly, and then enzymatically hydrolyze for 1.5 h to obtain the primary enzymatically hydrolyzed solution; adjust the pH of the primary enzymatically hydrolyzed solution to 6.0, add papain, enzymatically hydrolyze for 0.5 h, boil to inactivate the enzyme, cool, centrifuge, and dry the supernatant to obtain the extract of Enteromorpha prolifera.
[0022] Further, the addition amount of strontium nitrate in the step 2 is 10 mg / L, the addition amount of mussel polysaccharide is 10 g / L, and the addition amount of salicylic acid is 5 mg / L.
[0023] Further, the addition amount of cellulase in the step 3 is 3 g / L, the addition amount of snail enzyme is 5 g / L, and the addition amount of lysozyme is 0.5 g / L.
[0024] Furthermore, in the preparation method of the enteromorpha extract, the material-liquid ratio of enteromorpha to water is 1:3 kg / L.
[0025] Furthermore, in the preparation method of the enteromorpha extract, the addition amount of cellulase is 3% of the mass of enteromorpha.
[0026] Furthermore, in the preparation method of the enteromorpha extract, the addition amount of papain is 1% of the mass of enteromorpha.
[0027] The present invention provides the application of the growth regulator in improving plant stress resistance, and the plant stress resistance refers to resistance to low temperature stress.
[0028] Further, in the application, the using method of the growth regulator is to treat plant seeds by soaking or to treat plant seedlings in the seedling stage by irrigation. Description of the Drawings
[0029] Appendix Figure 1 : Changes in the MDA content in pepper seedlings in the low temperature seedling growth test;
[0030] Appendix Figure 2 : Changes in the root activity of pepper seedlings in the low temperature seedling growth test;
[0031] Appendix Figure 3 : Changes in the CAT activity in pepper seedlings in the low temperature seedling growth test.
[0032] The present invention has the following beneficial effects:
[0033] 1. Soaking pepper seeds with the growth regulator prepared by the present invention can improve the germination rate of pepper seeds after low temperature stress, promote the root development of seedlings and the growth of plants; treating pepper seedlings by irrigation can significantly promote the root growth of seedlings in a low temperature environment, effectively relieve the inhibitory effect of low temperature stress on the growth of seedlings, and reduce peroxidation products.
[0034] 2. In the plant growth regulator of the present invention, through the synergistic effect between different components, the tolerance performance of plants to low-temperature environments is jointly regulated. Among them, jasmonic acid can trigger the biological response of plants to harsh low-temperature environments and avoid cell damage; components such as betaine can promote plants to enhance their immune capabilities and protect plant cells from low-temperature damage; in the plant growth regulator of the present invention, components such as Paecilomyces hepiali fermentation products and polysaccharides of Irpex lacteus are creatively added, which can enhance the tolerance performance of plants to low-temperature stress, increase the seed germination rate under low-temperature stress, and promote the growth and development of plants. Detailed implementation manners
[0035] The present invention will be further illustrated below in conjunction with specific embodiments. It should be understood that these embodiments are only used to illustrate the present invention and not to limit the scope of the present invention. After reading the present invention, various equivalent modifications made by those skilled in the art to the present invention fall within the scope protected by the claims of this application. All kinds of hydrolases used in the present invention are commercially available products and can be obtained through various channels. Among them, the enzyme activity of cellulase is 50000U / g, the enzyme activity of lysozyme is 2000U / mg, and the enzyme activity of papain is 100000U / g.
[0036] Example 1
[0037] First, prepare the Zanthoxylum bungeanum leaf extract:
[0038] The preparation method of the Zanthoxylum bungeanum leaf extract is as follows:
[0039] Step A, after drying the Zanthoxylum bungeanum leaves, remove the volatile substances therein by steam distillation, and dry the distilled Zanthoxylum bungeanum leaves.
[0040] Step B, extract the distilled Zanthoxylum bungeanum leaves with a n-butanol-ethyl acetate mixed solution (10:2, v / v) for 10 h, filter, concentrate the filtrate under reduced pressure, remove the extraction solvent, and dry to obtain the Zanthoxylum bungeanum leaf extract.
[0041] Secondly, prepare the Enteromorpha prolifera extract:
[0042] Add 3 L of water to 1 kg of Enteromorpha prolifera and grind it into a homogenate. Add 30 g of cellulase and mix well, then enzymatically hydrolyze at 50 °C for 1.5 h to obtain a primary enzymatically hydrolyzed solution; adjust the pH of the primary enzymatically hydrolyzed solution to 6.0, then add 10 g of papain, enzymatically hydrolyze at 60 °C for 0.5 h, boil for 2 - 3 min to inactivate the enzyme, cool, centrifuge, and dry the supernatant to obtain the Enteromorpha prolifera extract.
[0043] Thirdly, prepare the microbial extract:
[0044] Step 1: After activating Paecilomyces hepiali with the preservation number CGMCC 3.12108 purchased from the China General Microbiological Culture Collection Center according to the instructions, inoculate it into the seed medium and culture until the OD value reaches 0.6 to obtain the Paecilomyces hepiali seed liquid; inoculate the Paecilomyces hepiali seed liquid into the medium containing 20 g / L of sucrose, 5 g / L of peptone, 5 g / L of Enteromorpha extract, 0.3 g / L of magnesium sulfate, and 0.8 g / L of potassium dihydrogen phosphate at an inoculation amount of 10% (v / v), and perform oscillating fermentation at 25°C and 500 rpm for 60 h to obtain the primary fermentation liquid.
[0045] Step 2: Add 10 mg / L of strontium nitrate, 10 g / L of mussel polysaccharide, and 5 mg / L of salicylic acid to the primary fermentation liquid, and then continue to perform oscillating fermentation at 25°C and 500 rpm for 6 h to obtain the final fermentation liquid.
[0046] Step 3: Add 3 g / L of cellulase, 5 g / L of snailase, and 0.5 g / L of lysozyme to the final fermentation liquid, perform enzymatic hydrolysis at 35°C for 1 h, heat to boiling to inactivate the enzyme, centrifuge, take the supernatant, and dry to obtain the microbial extract.
[0047] Finally, prepare the growth regulator:
[0048] Dissolve 0.5 g of jasmonic acid, 0.2 g of ligustilide, and 1.5 g of Zanthoxylum bungeanum leaf extract in an appropriate amount of ethanol to obtain Component A; dissolve 10 g of the microbial extract, 0.3 g of choline chloride, 15 g of fulvic acid potassium, 1.5 g of betaine, and 5 g of Irpex lacteus polysaccharide in 100 g of water, add 15 g of Tween-80 and mix evenly to obtain Component B; mix Component A and Component B to obtain the growth regulator.
[0049] Example 2
[0050] First, prepare the Zanthoxylum bungeanum leaf extract:
[0051] The preparation method of the Zanthoxylum bungeanum leaf extract is as follows:
[0052] Step A: After drying the Zanthoxylum bungeanum leaves, remove the volatile substances by steam distillation, and dry the distilled Zanthoxylum bungeanum leaves.
[0053] Step B: Extract the distilled Zanthoxylum bungeanum leaves with a n-butanol-ethyl acetate mixed solution (10:2, v / v) for 10 h, filter, concentrate the filtrate under reduced pressure, remove the extraction solvent, and dry to obtain the Zanthoxylum bungeanum leaf extract.
[0054] Secondly, prepare the Enteromorpha extract:
[0055] Add 1 kg of Enteromorpha prolifera to 3 L of water and grind to make a homogenate. Add 30 g of cellulase, mix well, and enzymatically hydrolyze at 50 °C for 1.5 h to obtain a primary enzymatically hydrolyzed solution. Adjust the pH of the primary enzymatically hydrolyzed solution to 6.0, then add 10 g of papain, enzymatically hydrolyze at 60 °C for 0.5 h, boil for 2 - 3 min to inactivate the enzyme, cool, centrifuge, and dry the supernatant to obtain the Enteromorpha prolifera extract.
[0056] Again, prepare the microbial extract:
[0057] Step 1: After activating Paecilomyces hepiali with the preservation number CGMCC 3.12108 purchased from the China General Microbiological Culture Collection Center according to the instructions, inoculate it into the seed medium and culture until the OD value reaches 0.6 to obtain the Paecilomyces hepiali seed liquid; inoculate the Paecilomyces hepiali seed liquid into the medium containing 20 g / L of sucrose, 5 g / L of peptone, 5 g / L of Enteromorpha prolifera extract, 0.3 g / L of magnesium sulfate, and 0.8 g / L of potassium dihydrogen phosphate at an inoculation amount of 10% (v / v), and oscillate and ferment at 25 °C and 500 rpm for 60 h to obtain a primary fermentation liquid;
[0058] Step 2: Add 10 mg / L of strontium nitrate, 10 g / L of mussel polysaccharide, and 5 mg / L of salicylic acid to the primary fermentation liquid, and then continue to oscillate and ferment at 25 °C and 500 rpm for 6 h to obtain a final fermentation liquid;
[0059] Step 3: Add 3 g / L of cellulase, 5 g / L of snail enzyme, and 0.5 g / L of lysozyme to the final fermentation liquid, enzymatically hydrolyze at 35 °C for 1 h, heat and boil to inactivate the enzyme, centrifuge, take the supernatant, and dry to obtain the microbial extract.
[0060] Finally, prepare the growth regulator:
[0061] Dissolve 0.3 g of jasmonic acid, 0.25 g of ligustilide, and 1 g of Zanthoxylum bungeanum leaf extract in an appropriate amount of ethanol to obtain Component A; dissolve 12 g of microbial extract, 0.1 g of choline chloride, 20 g of fulvic acid potassium, 1 g of betaine, and 8 g of Irpex lacteus polysaccharide in 100 g of water, add 10 g of Tween - 80 and mix evenly to obtain Component B; mix Component A and Component B to obtain the growth regulator.
[0062] Example 3
[0063] First, prepare the Zanthoxylum bungeanum leaf extract:
[0064] The preparation method of the Zanthoxylum bungeanum leaf extract is as follows:
[0065] Step A: After drying the Zanthoxylum bungeanum leaves, remove the volatile substances by steam distillation, and dry the distilled Zanthoxylum bungeanum leaves;
[0066] Step B: The distilled Zanthoxylum bungeanum leaves were extracted with a n-butanol-ethyl acetate mixed solution (10:2, v / v) for 10 h, filtered, the filtrate was concentrated under reduced pressure to remove the extraction solvent, and then dried to obtain the Zanthoxylum bungeanum leaf extract.
[0067] Secondly, prepare the Enteromorpha prolifera extract:
[0068] 1 kg of Enteromorpha prolifera was added with 3 L of water and ground into a homogenate. 30 g of cellulase was added and mixed evenly, and then enzymolyzed at 50 °C for 1.5 h to obtain a primary enzymolysis solution. The pH of the primary enzymolysis solution was adjusted to 6.0, then 10 g of papain was added, and enzymolyzed at 60 °C for 0.5 h, boiled for 2 - 3 min to inactivate the enzyme, cooled, centrifuged, and the supernatant was dried to obtain the Enteromorpha prolifera extract.
[0069] Thirdly, prepare the microbial extract:
[0070] Step 1: Paecilomyces hepiali purchased from the China General Microbiological Culture Collection Center with the preservation number CGMCC 3.12108 was activated according to the instructions, inoculated into the seed medium, and cultured until the OD value reached 0.6 to obtain the Paecilomyces hepiali seed liquid; the Paecilomyces hepiali seed liquid was inoculated into the medium containing 20 g / L of sucrose, 5 g / L of peptone, 5 g / L of Enteromorpha prolifera extract, 0.3 g / L of magnesium sulfate, and 0.8 g / L of potassium dihydrogen phosphate at an inoculation amount of 10% (v / v), and oscillating fermentation was carried out at 25 °C and 500 rpm for 60 h to obtain a primary fermentation liquid;
[0071] Step 2: 10 mg / L of strontium nitrate, 10 g / L of mussel polysaccharide, and 5 mg / L of salicylic acid were added to the primary fermentation liquid, and then oscillating fermentation was continued at 25 °C and 500 rpm for 6 h to obtain a final fermentation liquid;
[0072] Step 3: 3 g / L of cellulase, 5 g / L of snailase, and 0.5 g / L of lysozyme were added to the final fermentation liquid, enzymolyzed at 35 °C for 1 h, heated and boiled to inactivate the enzyme, centrifuged, the supernatant was taken, and dried to obtain the microbial extract.
[0073] Finally, prepare the growth regulator:
[0074] 0.4 g of jasmonic acid, 0.2 g of ligustilide, and 1.3 g of Zanthoxylum bungeanum leaf extract were dissolved in an appropriate amount of ethanol to obtain Component A; 11 g of microbial extract, 0.2 g of choline chloride, 18 g of fulvic acid potassium, 1.2 g of betaine, and 6 g of polysaccharide from Irpex lacteus were dissolved in 100 g of water, and then 13 g of Tween - 80 was added and mixed evenly to obtain Component B; Component A and Component B were mixed evenly to obtain the growth regulator.
[0075] Comparative Example 1
[0076] First, prepare the Zanthoxylum bungeanum leaf extract:
[0077] The preparation method of the Zanthoxylum bungeanum leaf extract is as follows:
[0078] Step A: After drying the Zanthoxylum bungeanum leaves, remove the volatile substances by steam distillation, and then dry the distilled Zanthoxylum bungeanum leaves;
[0079] Step B: Extract the distilled Zanthoxylum bungeanum leaves with a n-butanol-ethyl acetate mixed solution (10:2, v / v) for 10 h, filter, concentrate the filtrate under reduced pressure to remove the extraction solvent, and then dry to obtain the Zanthoxylum bungeanum leaf extract.
[0080] Secondly, prepare the growth regulator:
[0081] Dissolve 0.4 g of jasmonic acid, 0.2 g of ligustilide, and 1.3 g of Zanthoxylum bungeanum leaf extract in an appropriate amount of ethanol to obtain Component A; dissolve 0.2 g of choline chloride, 18 g of fulvic acid potassium, 1 mg of strontium nitrate, and 6 g of polysaccharide of Irpex lacteus in 100 g of water, add 13 g of Tween-80 and mix evenly to obtain Component B; mix Component A and Component B to obtain the growth regulator.
[0082] Comparative Example 2
[0083] First, prepare the Enteromorpha prolifera extract:
[0084] Grind 1 kg of Enteromorpha prolifera with 3 L of water to make a homogenate, add 30 g of cellulase and mix evenly, then enzymatically hydrolyze at 50 °C for 1.5 h to obtain a primary enzymolysis solution; adjust the pH of the primary enzymolysis solution to 6.0, add 10 g of papain, enzymatically hydrolyze at 60 °C for 0.5 h, boil for 2 - 3 min to inactivate the enzyme, cool, centrifuge, and dry the supernatant to obtain the Enteromorpha prolifera extract.
[0085] Secondly, prepare the microbial extract:
[0086] Step 1: After activating Paecilomyces hepiali with the preservation number CGMCC 3.12108 purchased from the China General Microbiological Culture Collection Center according to the instructions, inoculate it into the seed medium and culture until the OD value reaches 0.6 to obtain the Paecilomyces hepiali seed liquid; inoculate the Paecilomyces hepiali seed liquid into the medium containing 20 g / L of sucrose, 5 g / L of peptone, 5 g / L of Enteromorpha prolifera extract, 0.3 g / L of magnesium sulfate, and 0.8 g / L of potassium dihydrogen phosphate at an inoculation amount of 10% (v / v), and oscillate and ferment at 25 °C and 500 rpm for 60 h to obtain a primary fermentation liquid;
[0087] Step 2: Add 10 mg / L of strontium nitrate, 10 g / L of mussel polysaccharide, and 5 mg / L of salicylic acid to the primary fermentation liquid, and then continue to oscillate and ferment at 25 °C and 500 rpm for 6 h to obtain the final fermentation liquid;
[0088] Step 3: Add 3 g / L of cellulase, 5 g / L of snailase, and 0.5 g / L of lysozyme to the final fermentation broth, enzymatically hydrolyze at 35 °C for 1 h, inactivate the enzyme by heating to boiling, centrifuge, take the supernatant, and dry to obtain the microbial extract.
[0089] Finally, prepare the growth regulator:
[0090] Dissolve 0.4 g of jasmonic acid and 0.2 g of ligustilide in an appropriate amount of ethanol to obtain Component A; dissolve 11 g of the microbial extract, 18 g of potassium fulvate, and 1.2 g of betaine in 100 g of water, add 13 g of Tween-80 and mix well to obtain Component B; mix Component A and Component B to obtain the growth regulator.
[0091] Comparative Example 3
[0092] First, prepare the prickly ash leaf extract:
[0093] The preparation method of the prickly ash leaf extract is as follows:
[0094] Step A: After drying the prickly ash leaves, remove the volatile substances by steam distillation, and dry the distilled prickly ash leaves.
[0095] Step B: Extract the distilled prickly ash leaves with n-butanol for 10 h, filter, concentrate the filtrate under reduced pressure to remove the extraction solvent, and dry to obtain the prickly ash leaf extract.
[0096] Secondly, prepare the enteromorpha extract:
[0097] Grind 1 kg of enteromorpha with 3 L of water to make a homogenate, add 30 g of cellulase and mix well, enzymatically hydrolyze at 50 °C for 1.5 h to obtain the primary enzymatically hydrolyzed solution; adjust the pH of the primary enzymatically hydrolyzed solution to 6.0, then add 10 g of papain, enzymatically hydrolyze at 60 °C for 0.5 h, boil for 2 - 3 min to inactivate the enzyme, cool, centrifuge, and dry the supernatant to obtain the enteromorpha extract.
[0098] Thirdly, prepare the microbial extract:
[0099] Step 1: After activating Paecilomyces hepiali with the preservation number of CGMCC 3.12108 purchased from the China General Microbiological Culture Collection Center according to the instructions, inoculate it into the seed medium and culture until the OD value reaches 0.6 to obtain the Paecilomyces hepiali seed liquid; inoculate the Paecilomyces hepiali seed liquid at an inoculation amount of 10% (v / v) into a medium containing 20 g / L of sucrose, 5 g / L of peptone, 5 g / L of enteromorpha extract, 0.3 g / L of magnesium sulfate, and 0.8 g / L of potassium dihydrogen phosphate, and oscillate and ferment at 25 °C and 500 rpm for 60 h to obtain the fermentation broth;
[0100] Step 2: Add 3 g / L of cellulase, 5 g / L of snailase, and 0.5 g / L of lysozyme to the fermentation broth, enzymatically hydrolyze at 35 °C for 1 h, inactivate the enzyme by heating to boiling, centrifuge, take the supernatant, and dry it to obtain the microbial extract.
[0101] Finally, prepare the growth regulator:
[0102] Dissolve 0.4 g of jasmonic acid and 1.3 g of prickly ash leaf extract in an appropriate amount of ethanol to obtain Component A; dissolve 11 g of the microbial extract, 1 mg of strontium nitrate, 18 g of fulvic acid potassium, and 6 g of polysaccharide from Irpex lacteus in 100 g of water, add 13 g of Tween-80 and mix evenly to obtain Component B; mix Component A and Component B to obtain the growth regulator.
[0103] Performance test
[0104] I. Effects of the regulator on the germination and seedlings of pepper seeds under low-temperature environment
[0105] 1. Materials and methods
[0106] 1.1 Test materials
[0107] The pepper variety selected in this experiment is the commercially available "screw pepper" seeds, and the experiment is carried out in the greenhouse test base of our company.
[0108] 1.2 Test methods
[0109] This experiment is carried out with reference to existing literature.
[0110] Low-temperature germination test: A total of 7 treatments are set in this experiment. For each treatment, 50 seeds are selected. The pepper seeds are first soaked in the dark for 24 h. The soaked seeds are respectively placed on filter papers moistened with different regulator solutions (5 mL of regulator / 1 L of water), placed in a 4 °C refrigerator for 48 h, and then taken out and placed in a 25 °C climate incubator for conventional cultivation for 7 days to observe the seed germination and growth conditions.
[0111] Low-temperature seedling growth test: The pepper seeds are first soaked in the dark for 24 h, and the soaked seeds are placed in a 25 °C climate incubator for conventional cultivation. After germination, 7 treatments are set for the germinated seeds. For each treatment, 50 germinated seeds are selected. The germinated seeds are respectively placed in plug trays with a seedling substrate and cultivated conventionally in a 25 °C climate incubator. Every 2 days, 500 mL of different regulator solutions (containing 3 mL of regulator) are irrigated. When the pepper seedlings grow to two leaves and one heart, the temperature of the climate incubator is adjusted to 12 °C and cultivated for another 7 days to observe the growth conditions of the pepper seedlings.
[0112] 1.3 Determination indexes
[0113] 1.3.1 Root length: Randomly select 10 pepper sprouts in the low-temperature germination test and measure the length of the main root.
[0114] 1.3.2 Sprout height: Randomly select 10 pepper sprouts in the low-temperature germination test and measure the length from the cotyledon to the root collar of the sprout.
[0115] 1.3.3 Biochemical indexes: In the low-temperature seedling growth test, measure the content of malondialdehyde (MDA), root activity, and catalase (CAT) activity of pepper seedlings at the stage of two true leaves and one heart and during the cultivation at 12 °C. Select 5 pepper seedlings for each measurement.
[0116] 2. Results and analysis
[0117] 2.1 Effects of different regulators on pepper seed germination
[0118] As can be seen from the results in Table 1, when using the growth regulator of the present invention for seed germination, the germination rate of seeds after low-temperature treatment can be significantly improved, and the data of the root length and sprout height of the seedlings are significantly higher. The effect is better than that of the blank treatment group and the growth regulators obtained by using Comparative Examples 1-3.
[0119] Table 1 Comparison of pepper seed germination
[0120] Group Regulator Germination rate (%) Root length (cm) Shoot height (cm) Blank treatment 1 None 92 3.62±0.12 2.13±0.25 Treatment 1-1 Example 1 98 4.58±0.18 2.96±0.31 Treatment 1-2 Example 2 100 4.36±0.11 2.82±0.29 Treatment 1-3 Example 3 100 4.55±0.26 3.03±0.19 Treatment 1-4 Comparative example 1 96 3.98±0.17 2.51±0.24 Treatment 1-5 Comparative example 2 92 4.03±0.22 2.64±0.32 Treatment 1-6 Comparative example 3 94 3.84±0.15 2.41±0.27
[0121] 2.2 Effects of different regulators on pepper seedling growth
[0122] As can be seen from the appendix Figure 1-3 For the pepper seedlings treated with the growth regulator of the present invention, the content of MDA in the pepper plants shows a trend of first increasing and then decreasing, and the root activity shows a decreasing trend; the increasing trend of the content of MDA in the pepper plants is relatively slow, and the content of MDA is significantly lower than that of the pepper plants in the blank treatment group and the pepper plants treated with the growth regulators of Comparative Examples 1-3; the increase in CAT activity is obvious, which is better than that of the pepper plants in the blank treatment group and the pepper plants treated with the growth regulators of Comparative Examples 1-3; the decreasing trend of the root activity of the pepper seedlings treated with the growth regulator of the present invention is significantly slower.
[0123] The above test results show that the growth regulator of the present invention can effectively relieve the inhibitory effect of low-temperature stress on pepper seed germination and seedling growth, and reduce the peroxidation products.
Claims
1. A strontium-containing growth regulator, characterized in that, The growth regulator is composed of component A and component B. Component A contains 0.3 - 0.5 parts by weight of jasmonic acid, 0.2 - 0.25 parts by weight of ligustilide, and 1 - 1.5 parts by weight of pricklyash leaf extract. Component B contains 10 - 12 parts by weight of microbial extract, 0.1 - 0.3 parts by weight of choline chloride, 15 - 20 parts by weight of potassium fulvate, 1 - 1.5 parts by weight of betaine, 5 - 8 parts by weight of polysaccharide of irpex lacteus, 100 parts by weight of water, and 10 - 15 parts by weight of surfactant.
2. The growth regulator according to claim 1, wherein The preparation method of the growth regulator is as follows: Dissolve jasmonic acid, ligustilide, and pricklyash leaf extract in ethanol to obtain component A; dissolve microbial extract, choline chloride, potassium fulvate, betaine, and polysaccharide of irpex lacteus in water, and then add surfactant and mix evenly to obtain component B; mix component A and component B evenly to obtain the growth regulator.
3. The growth regulator according to claim 1 or 2, characterized in that, The preparation method of the pricklyash leaf extract in component A of the growth regulator is as follows: Step A: After drying the pricklyash leaves, remove the volatile substances by steam distillation, and dry the distilled pricklyash leaves. Step B: Extract the distilled pricklyash leaves with a mixed solution of n-butanol - ethyl acetate with a volume ratio of 10:2, filter, concentrate the filtrate under reduced pressure to remove the extraction solvent, and dry to obtain the pricklyash leaf extract.
4. The growth regulator according to claim 1 or 2, characterized in that The preparation method of the microbial extract in component B of the growth regulator is as follows: Step 1: Inoculate the Paecilomyces hepiali seed solution with an OD value of 0.6 into a medium containing 20 g / L of sucrose, 5 g / L of peptone, 5 g / L of enteromorpha extract, 0.3 g / L of magnesium sulfate, and 0.8 g / L of potassium dihydrogen phosphate, and oscillate and ferment at 25°C and 500 rpm for 60 h to obtain the primary fermentation broth. Step 2: Add strontium nitrate, polysaccharide of nacre, and salicylic acid to the primary fermentation broth, and then continue to oscillate and ferment at 25°C and 500 rpm for 6 h to obtain the final fermentation broth. Step 3: Add cellulase, snailase, and lysozyme to the final fermentation broth, enzymatically hydrolyze at 35°C for 1 h, heat to boiling to inactivate the enzyme, centrifuge, take the supernatant, and dry to obtain the microbial extract.
5. The growth regulator according to claim 4, wherein, The preparation method of the enteromorpha extract in Step 1 is as follows: Grind enteromorpha with water to make a homogenate, add cellulase and mix evenly, and then enzymatically hydrolyze for 1.5 h to obtain the primary enzymatically hydrolyzed solution; adjust the pH of the primary enzymatically hydrolyzed solution to 6.0, add papain, enzymatically hydrolyze for 0.5 h, boil to inactivate the enzyme, cool, centrifuge, and dry the supernatant to obtain the enteromorpha extract.
6. The growth regulator according to claim 4, wherein, The addition amount of strontium nitrate in Step 2 is 10 mg / L, the addition amount of polysaccharide of nacre is 10 g / L, and the addition amount of salicylic acid is 5 mg / L.
7. The growth regulator according to claim 4, wherein The addition amount of cellulase in Step 3 is 3 g / L, the addition amount of snailase is 5 g / L, and the addition amount of lysozyme is 0.5 g / L.
8. The growth regulator according to claim 5, characterized in that, In the preparation method of the enteromorpha extract, the addition amount of cellulase is 3% of the mass of enteromorpha, and the addition amount of papain is 1% of the mass of enteromorpha.
9. Application of a growth regulator according to any one of claims 1 - 8 in improving plant stress resistance.
10. The application according to claim 9, characterized in that, characterized in that, Treating the plant seeds by soaking them with the growth regulator or treating the plants at the seedling stage by irrigation can improve the tolerance of plants to low-temperature stress.