Feed additive for improving immunity of pet cats as well as preparation method and application of feed additive

Abstract

The invention provides a feed additive capable of improving immunity of pet cats as well as a preparation method and application of the feed additive, and belongs to the technical field of animal feed additives. The feed additive comprises probiotics and a traditional Chinese medicine extract, the probiotics comprise bifidobacterium animalis, combined lactobacillus salivarius and saccharomyces cerevisiae boulardii, and the traditional Chinese medicine extract comprises astragalus polysaccharide, curcumin and resveratrol. The probiotics directly enhance intestinal immunity through colonization resistance and metabolites, the traditional Chinese medicine components form internal and external dual defense through system immune activation, immunity-flora dual regulation is achieved, the probiotics and the traditional Chinese medicine extract form a symbiotic system of increasing the drug effect through bacteria and protecting the bacteria through drugs, and 1 + 1gt is achieved; and 2, the immunity improving effect is achieved.

Description

Technical Field

[0001] The present invention belongs to the technical field of animal feed additives, and particularly relates to a feed additive for improving the immunity of pet cats, a preparation method thereof, and an application thereof. Background Art

[0002] With the rapid development of the global pet industry, pet health issues have received increasing attention. Low pet immunity and stress response are the two core factors leading to their illness and shortened lifespan. Especially for pet cats, due to their unique physiological and behavioral characteristics, they are more vulnerable to external environmental changes (such as noise, unfamiliar environment, coexistence of multiple pets) and physiological state changes (such as pregnancy, aging, disease) compared to dogs, thus triggering chronic stress responses. Research shows that long-term stress can lead to immune suppression, intestinal barrier damage, and metabolic disorders in pets, significantly increasing the risk of infectious diseases and chronic inflammation in cats.

[0003] Currently, there is a lack of feed additives or diet feeds in the market for improving the immunity of pet cats. Common types include probiotic additives, vitamin additives, and attractant additives. However, although probiotics can regulate the intestinal flora of cats, the cat intestine is short and the flora abundance is low (only 1 / 3 of that of dogs), and the colonization efficiency of conventional strains is insufficient. Although high-dose vitamin E can enhance immunity, excessive intake will inhibit the absorption of taurine by cats and exacerbate the risk of cardiomyopathy. Although chemically synthesized flavors can improve palatability, they may induce oxidative stress and allergic reactions in cats.

[0004] As an obligate carnivore, the immune system of cats has significant specificity. Existing technologies mostly rely on single strains or chemically synthesized components, failing to effectively combine the synergistic mechanism of natural ingredients, or mostly focusing on basic nutrition supplementation, lacking functional design for stressors (such as environmental mutations, competition among multiple pets). Therefore, there is an urgent need to develop a feed additive and supporting feed for bidirectional regulation of immunity-stress in pet cats. Summary of the Invention

[0005] In view of this, the purpose of the present invention is to provide a feed additive for improving the immunity of pet cats, a preparation method thereof, and an application thereof, which can enhance the immunity of pet cats and reduce stress damage to pet cats through a composite probiotic-traditional Chinese medicine active ingredient synergistic system.

[0006] To achieve the above-mentioned invention purpose, the present invention provides the following technical solutions:

[0007] A feed additive for improving the immunity of pet cats, comprising probiotics and traditional Chinese medicine extracts; the probiotics include Bifidobacterium animalis, Lactobacillus salivarius subsp. salicinius, and Saccharomyces boulardii, and the traditional Chinese medicine extracts include astragalus polysaccharide, curcumin, and resveratrol.

[0008] Preferably, the mass ratio of Bifidobacterium animalis, Lactobacillus salivarius subsp. salicinius and Saccharomyces boulardii is 2-4:1-3:1.

[0009] Preferably, the mass ratio of astragalus polysaccharide, curcumin and resveratrol is 4-6:2-4:1.

[0010] Preferably, it includes 30-60 wt% of probiotics, 20-40 wt% of traditional Chinese medicine extract, and the balance of excipients.

[0011] More preferably, the excipients are selected from one or more of maltodextrin, trehalose, silicon dioxide and microcrystalline cellulose.

[0012] The present invention also provides a preparation method of the feed additive, comprising the following steps:

[0013] (1) Bifidobacterium animalis, Lactobacillus salivarius subsp. salicinius and Saccharomyces boulardii are respectively activated and cultured until the viable count of the bacterial liquid ≥ 1×10 9 CFU / mL, the bacterial liquid is centrifuged to obtain bacterial sludge, and the bacterial sludge is vacuum freeze-dried and then mixed to obtain probiotic powder; (2) astragalus polysaccharide, curcumin and resveratrol are mixed to obtain a traditional Chinese medicine extract; (3) the probiotic powder, the traditional Chinese medicine extract and the excipients are mixed and granulated to obtain the feed additive.

[0014] Preferably, the particle size of the feed additive is 0.2-0.5 mm, and the water content ≤ 5%.

[0015] The present invention also provides the application of the feed additive in the preparation of pet cat daily diet feed.

[0016] Preferably, the addition amount of the feed additive is 0.5-1.5 wt% of the pet cat daily diet feed.

[0017] The present invention also provides a daily diet feed for improving the immunity of pet cats, comprising the following components: 65-75 wt% of animal protein, 10-20 wt% of low-carbohydrate filler, 8-12 wt% of animal fat, 3-6 wt% of nutrients, and 0.5-1.5 wt% of the feed additive.

[0018] Compared with the prior art, the present invention has the following beneficial effects:

[0019] The feed additive of the present invention adopts an innovative system of the synergism of compound probiotics and Chinese herbal extracts. Bifidobacterium animalis, Lactobacillus salivarius and Saccharomyces boulardii in the probiotics act in multiple aspects such as inhibiting pathogenic bacteria, regulating neuroendocrine, adsorbing toxins, etc., directly enhancing intestinal immunity; Astragalus polysaccharide, curcumin and resveratrol in the Chinese herbal extracts activate macrophages, inhibit inflammatory mediators, and regulate the endocrine axis, realizing systemic immune activation, forming an internal and external double defense. This synergistic effect realizes the dual regulation of immunity-microflora, constructs a symbiotic system of "bacteria enhancing the efficacy of medicine, and medicine protecting the activity of bacteria", and achieves an immune enhancement effect of 1+1>2.

[0020] The feed additive of the present invention can effectively reduce the stress response of pet cats and reduce the damage to the body caused by stress; it can significantly improve the intestinal flora structure, promote the production of beneficial short-chain fatty acids, effectively maintain the intestinal barrier function, and reduce intestinal damage. Moreover, the feed additive of the present invention will not have an adverse effect on the body of pet cats during use, and has good safety, providing a reliable guarantee for the long-term consumption of pet cats. Detailed implementation mode

[0021] The present invention provides a feed additive for improving the immunity of pet cats, comprising probiotics and Chinese herbal extracts; the probiotics include Bifidobacterium animalis, Lactobacillus salivarius and Saccharomyces boulardii, and the Chinese herbal extracts include Astragalus polysaccharide, curcumin and resveratrol.

[0022] In the present invention, preferably, the mass ratio of Bifidobacterium animalis, Lactobacillus salivarius and Saccharomyces boulardii is 2-4:1-3:1, and more preferably 3:2:1. In the present invention, Bifidobacterium animalis inhibits the proliferation of pathogenic bacteria by competitive occupation, maintains the diversity of intestinal microorganisms, and reduces the abundance of pathogenic bacteria; stimulates the intestinal mucosal immune system, promotes the secretion of immunoglobulins, and enhances the local and systemic immune responses of the intestine. Lactobacillus salivarius regulates the neuroendocrine system through the gut-brain axis, reduces the level of stress hormones (cortisol), and alleviates anxiety behaviors caused by environmental changes; enhances the thickness of the mucus layer, reduces intestinal epithelial cell damage, and prevents the translocation of toxins and pathogens. Saccharomyces boulardii can adsorb mycotoxins and metabolic wastes in the feed, reduce the detoxification burden of the liver; decompose macromolecular allergenic proteins (such as gluten, milk protein), and reduce the risk of food allergy. Bifidobacterium animalis dominates the colonization of the colon, Lactobacillus salivarius strengthens the defense of the small intestine, and Saccharomyces boulardii covers the whole intestine, which can form a multi-level protection barrier.

[0023] The present invention has no special limitation on the specific sources of Bifidobacterium animalis, Lactobacillus salivarius and Saccharomyces boulardii, and they can be directly purchased from the market.

[0024] In the present invention, the mass ratio of astragalus polysaccharide, curcumin and resveratrol is preferably 4-6:2-4:1, and more preferably 5:3:1. In the present invention, astragalus polysaccharide can activate macrophages and natural killer cells, enhance the non-specific immune clearance ability, and can also block the binding of viruses to host cells, inhibiting the replication of feline coronavirus, herpes virus, etc. Curcumin can inhibit the release of pro-inflammatory mediators, relieve chronic intestinal inflammation and skin allergic reactions; and scavenge free radicals, protect the functions of cell membranes and mitochondria, and reduce the accumulation of oxidative damage. Resveratrol enhances the body's stress resistance by regulating the hypothalamic-pituitary-adrenal axis, and can also promote the differentiation of memory T / B cells, improving the vaccine response efficiency and the ability to defend against secondary infections.

[0025] The present invention has no special limitation on the sources of astragalus polysaccharide, curcumin and resveratrol, which can be directly purchased from the market, or can be extracted and purified by referring to the common preparation methods in the technical field. As an implementable mode, astragalus polysaccharide is extracted by the water extraction and alcohol precipitation method, curcumin is extracted by supercritical CO2 extraction, and resveratrol is extracted by ultrasonic-assisted extraction from grape skins.

[0026] In the present invention, probiotics directly enhance intestinal immunity through colonization resistance and metabolites, and traditional Chinese medicine components form a dual defense inside and outside through systemic immune activation; traditional Chinese medicine extracts promote the proliferation of probiotics, and probiotics metabolize traditional Chinese medicine components to generate active small molecules, improving bioavailability and realizing dual regulation of immunity and flora. Probiotics and traditional Chinese medicine extracts form a symbiotic system of "bacteria enhancing the efficacy of medicine, and medicine protecting the activity of bacteria", achieving an immune enhancement effect of 1+1>2. Moreover, the probiotics of the present invention are bile salt / gastric acid resistant, and are adapted to the high-protein requirements and sensitive intestinal characteristics of cats.

[0027] In the present invention, preferably, probiotics are 30-60 wt%, traditional Chinese medicine extracts are 20-40 wt%, and the balance is excipients; more preferably, probiotics are 40-50 wt%, traditional Chinese medicine extracts are 25-35 wt%, and the balance is excipients; even more preferably, probiotics are 45 wt%, traditional Chinese medicine extracts are 30 wt%, and excipients are 25 wt%.

[0028] In the present invention, preferably, the excipients are selected from one or more of maltodextrin, trehalose, silicon dioxide and microcrystalline cellulose; more preferably, the excipients are maltodextrin, trehalose and silicon dioxide, and the mass ratio is 15-20:6-10:1; even more preferably, the mass ratio of maltodextrin, trehalose and silicon dioxide is 16:8:1. As an implementable mode, the formula of a feed additive for improving the immunity of pet cats is: probiotics 45 wt%, traditional Chinese medicine extracts 30 wt%, maltodextrin 16 wt%, trehalose 8 wt% and silicon dioxide 1 wt%.

[0029] The present invention also provides a preparation method of the feed additive, comprising the following steps:

[0030] (1)Bifidobacterium animalis, Lactobacillus salivarius subsp. salivarius, and Saccharomyces boulardii are respectively activated and cultured until the viable count of the bacterial liquid ≥ 1×10 9 CFU / mL. The bacterial liquid is centrifuged to obtain bacterial sludge, and the bacterial sludge is vacuum freeze-dried and then mixed to obtain probiotic powder;

[0031] (2)Astragalus polysaccharide, curcumin, and resveratrol are mixed to obtain a traditional Chinese medicine extract;

[0032] (3)The probiotic powder, traditional Chinese medicine extract, and excipients are mixed and granulated to obtain a feed additive.

[0033] The present invention has no special limitation on the activation and culture method, and the common liquid activation and culture method in the technical field can be used. As an implementable mode, Bifidobacterium animalis and Lactobacillus salivarius subsp. salivarius are cultured anaerobically at 37 °C using MRS liquid medium; Saccharomyces boulardii is cultured with shaking at 30 °C and 180 rpm using YPD liquid medium. The plate counting method is used to ensure that the viable count of the bacterial liquid ≥ 1×10 9 CFU / mL.

[0034] In the present invention, the preferred centrifugation speed of the bacterial liquid is 3000 - 5000 rpm, and more preferably 4000 rpm; the preferred centrifugation time of the bacterial liquid is 10 - 20 min, and more preferably 15 min for Bifidobacterium animalis and Lactobacillus salivarius subsp. salivarius, and 10 min for Saccharomyces boulardii. It is preferred to wash the bacterial sludge twice with sterilized normal saline, and the vacuum freeze-drying conditions are pre-freezing at -40 °C and vacuum drying at -50 °C for 24 h.

[0035] In the present invention, it is preferred to use fluidized bed spray granulation for granulation; further preferably, the inlet air temperature for granulation is 40 - 60 °C, more preferably 50 °C; further preferably, the atomization pressure is 0.3 - 0.5 MPa, more preferably 0.4 MPa.

[0036] In the present invention, the preferred particle size of the feed additive is 0.2 - 0.5 mm, more preferably 0.3 - 0.4 mm, and the water content ≤ 5%.

[0037] The present invention also provides the application of the feed additive in the preparation of pet cat daily diet feed; preferably, the addition amount of the feed additive is 0.5 - 1.5 wt% of the pet cat daily diet feed, more preferably 0.8 - 1.2 wt%, and most preferably 1 wt%.

[0038] The present invention also provides a daily diet feed for improving the immunity of pet cats, comprising the following components: 65 - 75 wt% of animal protein, 10 - 20 wt% of low-carbohydrate filler, 8 - 12 wt% of animal fat, 3 - 6 wt% of nutrients, and 0.5 - 1.5 wt% of the feed additive.

[0039] In the present invention, animal protein is preferably 70 wt%, and more preferably the animal protein is selected from one or more of chicken meal, beef meal, salmon meal, hydrolyzed chicken liver protein, and egg white powder; low-carbohydrate filler is preferably 15 wt%, and more preferably the low-carbohydrate filler is selected from one or more of pea flour, potato flour, tapioca starch, and chickpea flour; animal fat is preferably 10 wt%, and more preferably the animal fat is selected from one or more of chicken oil, duck oil, fish oil, and medium-chain triglycerides; nutrients are preferably 4 wt%, and more preferably the nutrients are selected from one or more of vitamin / mineral premix, taurine, amino acids (such as L-arginine), and fructooligosaccharide.

[0040] The technical solutions provided by the present invention will be described in detail below in conjunction with the embodiments, but they should not be construed as limiting the protection scope of the present invention.

[0041] Example 1

[0042] A feed additive for improving the immunity of pet cats, the formula and preparation method are as follows:

[0043] Formula: Probiotics 45 wt% (the mass ratio of Bifidobacterium animalis, Lactobacillus salivarius, and Saccharomyces boulardii is = 3:2:1), Chinese herbal medicine extract 30 wt% (the mass ratio of astragalus polysaccharide, curcumin, and resveratrol is = 5:3:1), maltodextrin 16 wt%, trehalose 8 wt%, and silicon dioxide 1 wt%.

[0044] Preparation method:

[0045] (1) Inoculate Bifidobacterium animalis and Lactobacillus salivarius into MRS liquid medium respectively and culture anaerobically at 37 °C; inoculate Saccharomyces boulardii into YPD liquid medium and culture with shaking at 30 °C and 180 rpm. Monitor by plate counting method. After the viable cell count of the bacterial liquid is ≥ 1×10 9 CFU / mL, centrifuge the bacterial liquid at 4000 rpm. Centrifuge Bifidobacterium animalis and Lactobacillus salivarius for 15 min, and centrifuge Saccharomyces boulardii for 10 min to obtain bacterial sludge. Wash the bacterial sludge twice with sterilized normal saline, pre-freeze at -40 °C, and vacuum dry at -50 °C for 24 h, and mix according to the formula ratio to obtain probiotic powder.

[0046] (2) Weigh astragalus polysaccharide, curcumin, and resveratrol according to the formula ratio and mix them thoroughly to obtain the Chinese herbal medicine extract.

[0047] (3) Mix the probiotic powder, Chinese herbal medicine extract with maltodextrin, trehalose, and silicon dioxide evenly, and use fluidized bed spray granulation. The inlet air temperature is 50 °C, the atomization pressure is 0.4 MPa, and a feed additive with a particle size of 0.3 - 0.4 mm and a water content of ≤ 5% is prepared.

[0048] Example 2

[0049] A feed additive for improving the immunity of pet cats, the formula and preparation method are as follows:

[0050] Formula: Probiotics 30wt% (the mass ratio of Bifidobacterium animalis, Lactobacillus salivarius, and Saccharomyces boulardii is 2:1:1), traditional Chinese medicine extract 40wt% (the mass ratio of astragalus polysaccharide, curcumin, and resveratrol is 4:2:1), microcrystalline cellulose 30wt%.

[0051] Preparation method: (1) The steps of activation culture and preparation of probiotic powder are the same as in Example 1, and the culture time is adjusted to ensure that the viable count of the bacterial liquid meets the standard. (2) Mix astragalus polysaccharide, curcumin, and resveratrol to obtain the traditional Chinese medicine extract. (3) Mix the above raw materials and use fluidized bed spray granulation to control the inlet air temperature at 40°C and the atomization pressure at 0.5 MPa to prepare a feed additive with a particle size of 0.2 - 0.3 mm and a water content of ≤5%.

[0052] Example 3

[0053] A feed additive for improving the immunity of pet cats, the formula and preparation method are as follows:

[0054] Formula: Probiotics 60wt% (the mass ratio of Bifidobacterium animalis, Lactobacillus salivarius, and Saccharomyces boulardii is 4:3:1), traditional Chinese medicine extract 20wt% (the mass ratio of astragalus polysaccharide, curcumin, and resveratrol is 6:4:1), maltodextrin 15wt%, trehalose 4wt%, silicon dioxide 1wt%.

[0055] Preparation method: (1) The steps of activation culture and preparation of probiotic powder are the same as in Example 1, and the culture time is adjusted to ensure that the viable count of the bacterial liquid meets the standard. (2) Mix astragalus polysaccharide, curcumin, and resveratrol to obtain the traditional Chinese medicine extract. (3) Mix the above raw materials and use fluidized bed spray granulation to control the inlet air temperature at 60°C and the atomization pressure at 0.3 MPa to prepare a feed additive with a particle size of 0.4 - 0.5 mm and a water content of ≤5%.

[0056] Example 4

[0057] A daily ration feed for improving the immunity of pet cats, the formula and preparation method are as follows:

[0058] Formula: Chicken meal 40wt%, beef meal 30wt%, pea flour 15wt%, chicken oil 10wt%, vitamin / mineral premix 3wt%, taurine 1wt%, and the feed additive prepared in Example 1 1wt%.

[0059] Preparation method: Mix chicken powder and beef powder evenly. Add pea starch and chicken fat, and stir evenly. Then add vitamin / mineral premix, taurine and feed additive, mix well, granulate, and control the water content of the granules ≤ 10%.

[0060] Example 5

[0061] A daily diet feed for improving the immunity of pet cats, with the following formula:

[0062] Formula: 35 wt% salmon powder, 35 wt% hydrolyzed chicken liver protein, 15 wt% potato starch, 10 wt% fish oil, 2 wt% amino acid, 1.5 wt% fructooligosaccharide, 1.5 wt% feed additive prepared in Example 2.

[0063] The preparation method is the same as that in Example 4.

[0064] Example 6

[0065] A daily diet feed for improving the immunity of pet cats, with the following formula:

[0066] Formula: 70 wt% egg white powder, 15 wt% tapioca starch, 10 wt% duck fat, 3.5 wt% vitamin / mineral premix, 1 wt% taurine, 0.5 wt% feed additive prepared in Example 3.

[0067] The preparation method is the same as that in Example 4.

[0068] Test Example 1

[0069] 1. Test design

[0070] (1) Experimental animals: 48 healthy adult domestic cats (aged 1 - 2 years, weight 3.5 ± 0.5 kg), without immune system diseases and intestinal abnormalities after physical examination.

[0071] (2) Grouping design:

[0072] Control group: Basic diet (commercially available full-price cat food);

[0073] Existing technology group 1: Basic diet + 0.12 wt% Saccharomyces boulardii: Pediococcus acidilactici: yeast derivative Yang = 10:10:80 (from CN 113142383 A);

[0074] Existing technology group 2: Basic diet + Lactobacillus acidophilus: Bifidobacterium longum: Rhodopseudomonas palustris = 9.0×10 8 CFU / g: 6.5×10 8 CFU / g: 3.5×10 8 CFU / g (from Group 3 of "Effect of Compound Probiotic Preparation on the Immunity of Pet Dogs and Cats");

[0075] Probiotic group: basal diet + 1 wt% probiotics (Bifidobacterium animalis: Lactobacillus salivarius: Saccharomyces boulardii = 3:2:1);

[0076] Traditional Chinese medicine extract group: basal diet + 1 wt% traditional Chinese medicine extract (astragalus polysaccharide: curcumin: resveratrol = 5:3:1);

[0077] Composite group: basal diet + 1 wt% feed additive of Example 1 (Bifidobacterium animalis: Lactobacillus salivarius: Saccharomyces boulardii = 3:2:1 + astragalus polysaccharide: curcumin: resveratrol = 5:3:1). The strain number of Bifidobacterium animalis is CGMCC No. 5470, purchased from the China General Microbiological Culture Collection Center; the strain number of Lactobacillus salivarius is CICC 23179, purchased from the China Center of Industrial Culture Collection; the strain number of Saccharomyces boulardii is CGMCC NO. 18938, purchased from China Agricultural University.

[0078] (3) Test period: continuous feeding for 4 weeks, free access to food, and acute stress (strange environment + continuous noise for 24 h) was carried out in the 3rd week.

[0079] (4) Feeding management: This experiment was carried out in a certain pet nutrition research center in Nanjing, and routine disinfection was carried out before the official start of the experiment. During the entire experimental period, feeding was strictly carried out according to the grouping design, the cat house was kept well ventilated, the temperature and humidity were unified, and feeding and cleaning were carried out according to the routine management procedures of the cat house.

[0080] 2. Index detection

[0081] (1) Detection of feed additive safety indicators:

[0082] On the 0th, 7th, 14th, and 21st days of the experiment, the body weight, food intake, and diarrhea rate of each group were detected. Before the stress treatment in the 3rd week, the blood routine (white blood cell count, hematocrit) and liver and kidney functions (ALT, AST, BUN) of each group were detected.

[0083] (2) Detection of immunity and stress indicators:

[0084] After the acute stress in the 3rd week, serum was collected from each group, and serum cortisol was detected by ELISA method (commercial kit). In the 4th week, 0.5 mL of anterior limb venous blood was collected from each group, and the serum IgG content was detected by enzyme-linked immunosorbent assay (ELISA kit); peripheral blood mononuclear cells were collected, and the NK cell activity was detected by lactate dehydrogenase release method (LDH method); serum was collected, and the malondialdehyde (MDA) content was detected by colorimetric method.

[0085] (3) Detection of intestinal health indicators:

[0086] In the 4th week, fresh feces of each group were collected, and the proportion of Bifidobacterium in the fecal flora was detected by 16S rRNA sequencing (V3-V4 region); the fresh feces were freeze-dried, and the content of short-chain fatty acids (butyric acid) was detected by headspace gas chromatography-mass spectrometry (GC-MS); serum and feces were collected respectively, and intestinal barrier markers were detected by serum DAO (diamine oxidase) + fecal calprotectin (ELISA).

[0087] 3. Test Results

[0088] The body weights, feed intakes and diarrhea rates of cats in each treatment group were normal. In addition, as shown in Table 1, the safety indexes of each feed additive in different treatment groups were within the normal reference ranges. This indicates that during the test period, neither the feed additive of the present invention (compound group) nor the additives used in other control groups had adverse effects on the blood routine (white blood cell count, hematocrit) and liver and kidney functions (ALT, AST, BUN) of pet cats, that is, each additive performed well in terms of safety and did not cause adverse reactions in the bodies of pet cats, providing a safety guarantee for the further application of the feed additive of the present invention.

[0089] Table 1 Safety indexes of each feed additive in different treatment groups

[0090]

[0091] As shown in Table 2, in terms of immune and stress indexes, compared with the control group, the serum IgG level and NK cell activity in each treatment group were significantly increased (P<0.05), the cortisol level was significantly decreased (P<0.05), and the SOD activity was significantly increased (P<0.05). Among them, the improvement or reduction effect of the compound group was the most obvious, the serum IgG level was significantly higher than that of the probiotic group and the traditional Chinese medicine extract group (P<0.05), and the SOD activity was also significantly higher than that of other groups (P<0.05). This shows that the feed additive of the present invention (compound group) has more significant effects than the single probiotic group, traditional Chinese medicine extract group and the prior art group in improving the immunity of pet cats, enhancing the body's immune response, relieving stress reactions and improving antioxidant capacity, realizing two-way regulation of immunity-stress.

[0092] Table 2 Immune and stress index levels of pet cats in different treatment groups

[0093]

[0094] Note: * indicates significant difference compared with the control group (P<0.05); ** indicates extremely significant difference compared with the control group (P<0.01); ▲ indicates significant difference compared with the probiotic group (P<0.05); # indicates significant difference compared with the traditional Chinese medicine extract group (P<0.05).

[0095] As shown in Table 3, in terms of intestinal health indicators, the proportion of Bifidobacterium and the butyric acid content in the composite group were significantly higher than those in other groups (P<0.05), while the serum DAO was significantly lower than that in other groups (P<0.05), and the fecal calprotectin content was significantly lower than that in the control group and the traditional Chinese medicine extract group (P<0.05). This indicates that the feed additive (composite group) of the present invention can significantly improve the intestinal flora structure of pet cats, increase the proportion of the beneficial bacterium Bifidobacterium, promote the production of short-chain fatty acids (butyric acid), and at the same time effectively maintain the intestinal barrier function and reduce intestinal damage, being superior to the single probiotic group, the traditional Chinese medicine extract group and the prior art group, further reflecting the technical advantage of the present invention in improving the immunity of pet cats through dual regulation of immunity and flora.

[0096] Table 3 Intestinal health index levels of pet cats in different treatment groups

[0097]

[0098]

[0099] Note: * indicates significant difference compared with the control group (P<0.05); ** indicates extremely significant difference compared with the control group (P<0.01); ▲ indicates significant difference compared with the probiotic group (P<0.05); # indicates significant difference compared with the traditional Chinese medicine extract group (P<0.05).

[0100] The above are only the preferred embodiments of the present invention. It should be noted that for those of ordinary skill in the art, without departing from the principle of the present invention, several improvements and refinements can still be made, and these improvements and refinements should also be regarded as the protection scope of the present invention.

Claims

1. A feed additive for improving the immunity of pet cats, characterized in that, It includes probiotics and traditional Chinese medicine extracts; the probiotics include Bifidobacterium animalis, Lactobacillus salivarius and Saccharomyces boulardii, and the traditional Chinese medicine extracts include astragalus polysaccharide, curcumin and resveratrol.

2. The feed additive according to claim 1, characterized in that, The mass ratio of Bifidobacterium animalis, Lactobacillus salivarius and Saccharomyces boulardii is 2-4:1-3:

1.

3. The feed additive according to claim 1, wherein The mass ratio of astragalus polysaccharide, curcumin and resveratrol is 4-6:2-4:

1.

4. The feed additive according to claim 1, characterized in that, It includes 30-60wt% of probiotics, 20-40wt% of traditional Chinese medicine extracts, and the balance is excipients.

5. The feed additive according to claim 4, wherein The excipients are selected from one or more of maltodextrin, trehalose, silicon dioxide and microcrystalline cellulose.

6. The preparation method of the feed additive according to any one of claims 1-5, characterized in that, It includes the following steps: (1)Bifidobacterium animalis, Lactobacillus salivarius subsp. salivarius, and Saccharomyces boulardii were separately activated and cultured until the viable cell count of the bacterial liquid was ≥ 1 × 10 9 CFU / mL. The bacterial liquid was centrifuged to obtain bacterial sludge, and the bacterial sludge was vacuum freeze-dried and then mixed to obtain probiotic powder; (2) Mix astragalus polysaccharide, curcumin and resveratrol to obtain traditional Chinese medicine extracts; (3) Mix probiotic powder, traditional Chinese medicine extracts and excipients and granulate to obtain a feed additive.

7. The preparation method according to claim 6, characterized in that, The particle size of the feed additive is 0.2-0.5mm, and the water content ≤ 5%.

8. Use of the feed additive according to any one of claims 1-5 in the preparation of a pet cat daily diet feed.

9. The application according to claim 8, characterized in that, The addition amount of the feed additive is 0.5-1.5wt% of the pet cat daily diet feed.

10. A daily diet feed for improving the immunity of pet cats, characterized in that, It includes the following components: 65-75wt% of animal protein, 10-20wt% of low-carbohydrate filler, 8-12wt% of animal fat, 3-6wt% of nutrients, and 0.5-1.5wt% of the feed additive according to any one of claims 1-5.

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