Traditional Chinese medicine enzyme for reducing in-vivo accumulation of acetaldehyde after drinking and preparation method of traditional Chinese medicine enzyme

Abstract

The invention discloses a traditional Chinese medicine enzyme for reducing in-vivo accumulation of acetaldehyde after drinking and a preparation method of the traditional Chinese medicine enzyme, and belongs to the technical field of food enzyme preparation. The preparation method comprises the following steps: adding a compound enzyme into a Chinese waxgourd peel, kudzuvine root, hovenia dulcis thunb, dark plum, liquorice and mung bean compound for enzymolysis treatment to obtain an enzymolysis extract; adding composite engineering bacteria into the enzymolysis extract for fermentation treatment to obtain an enzyme stock solution; and adding water into the enzyme stock solution for dilution, and finally adding auxiliary materials to prepare the wine type medicinal and edible dual-purpose enzyme. According to the preparation method disclosed by the invention, through organic integration of enzymolysis treatment and fermentation processes, and effective control of the action temperature and time of enzymolysis and fermentation in the preparation process, the problems of too long preparation time and hidden dangers in the aspects of safety and functionality of traditional enzymes are effectively solved; the preparation method is simple and convenient in process and controllable in operation, and the fermentation time is remarkably shortened; the prepared traditional Chinese medicine enzyme has the health-care function of reducing the accumulation of acetaldehyde in the body after drinking.

Description

Technical Field

[0001] The present invention relates to the technical field of food enzyme preparation, and specifically relates to a traditional Chinese medicine enzyme for reducing the accumulation of acetaldehyde in the body after drinking and a preparation method thereof. Background Art

[0002] China's liquor culture has a long history and is an important part of traditional Chinese culture. Chinese liquor is the king of all medicines, which can promote the circulation of qi and the rising of yang qi. When consumed moderately, it plays an important role in maintaining health. However, Western scientific research has found that drinking is harmful to health, especially for those with alcohol allergies. Drinking has a great impact on health, thus causing serious negative effects on the spread of Chinese liquor and Chinese liquor culture. What is the effect of drinking on health? Let's start from the metabolic process of alcohol. Among the ethanol absorbed from drinking, about 90-95% is metabolized in the liver, and only 2%-10% of ethanol is excreted from the body in the form of ethanol through the kidneys, lungs, sweat, etc. After ethanol in liquor enters the human body, it is quickly absorbed through organs such as the mouth, esophagus, stomach, and intestines, and is rapidly transported to various tissues and organs throughout the body for metabolism. Among them, the ethanol absorbed by the lower part of the stomach and the intestines enters the liver, and under the action of ethanol dehydrogenase in the liver, it is converted into acetaldehyde, and then under the action of acetaldehyde dehydrogenase, it is converted into acetic acid, and then further oxidized into carbon dioxide and water, releasing energy. The acetaldehyde generated is the direct agent causing liver damage. Acetaldehyde can combine with proteins to form an acetaldehyde complex, resulting in the loss of protein function, inducing fibrosis formation, and inducing abnormal immune responses, leading to damage to liver cells. Acetaldehyde can also form superoxides under the action of xanthine oxidase, causing lipid peroxidation, leading to liver damage and causing degeneration of liver cells. Acetaldehyde can also damage the mitochondria in liver cells, reducing the fatty acid decomposition function of liver cells, leading to fatty liver. Acetaldehyde can also cause a lack of the activity of reduced glutathione (GSH) in mitochondria, so that the O2- free radicals generated by the respiratory chain cannot be transported in time, destroying the activity of superoxide dismutase SOD, and the O2- free radicals cannot form H2O2, resulting in lipid peroxidation reactions in mitochondria. In addition, a part of ethanol is metabolized through microsomes, and cytochrome P450 plays a major role in the ethanol microsomal oxidation system, which is involved in the metabolism of various compounds (including carcinogens).

[0003] Therefore, what is really harmful to human health is not alcohol, but its metabolic intermediate product acetaldehyde. Alcohol allergy or damage to health caused by drinking is mainly due to the lack of acetaldehyde-metabolizing enzymes or insufficient activity of acetaldehyde-metabolizing enzymes, and at the same time, the activity of ethanol dehydrogenase is too high, resulting in excessive accumulation of acetaldehyde in the body after drinking. As long as the activity of ethanol dehydrogenase can be inhibited and the activity of acetaldehyde dehydrogenase can be increased, the generation of acetaldehyde can be reduced, the conversion of acetaldehyde can be enhanced, the accumulation in the body can be reduced, and thus the harm of drinking to health can be reduced, while retaining its functions of promoting blood circulation and removing blood stasis, enhancing human yang qi, relieving stress, and making people feel happy, that is, relieving alcohol toxicity and preserving the benefits of liquor.

[0004] The peel of wax gourd belongs to the lung, spleen, and small intestine meridians and has the effect of promoting diuresis and reducing swelling. Kudzu root is the dried root of Pueraria lobata (Willd.) Ohwi, commonly known as wild kudzu. Its properties and flavors are sweet, pungent, and cool, and it has the functions of relieving muscle fever, promoting eruption, promoting fluid production to quench thirst, and ascending yang to stop diarrhea. It is commonly used for exterior syndrome fever, stiffness and pain in the nape and back, unsmooth measles, thirst in febrile diseases, yin deficiency with polydipsia, febrile diarrhea, and diarrhea due to spleen deficiency. Kudzu root is rich in puerarin, which has the effects of enhancing immunity, strengthening myocardial contractility, protecting myocardial cells, lowering blood pressure, and antiplatelet aggregation. Its specific efficacy is to improve the regenerative ability of liver cells, restore normal liver function, promote bile secretion, and prevent fat accumulation in the liver; promote metabolism, enhance the liver's detoxification function, and prevent alcohol damage to the liver.

[0005] Hovenia dulcis Thunb. is also known as the longevity fruit. It tastes sweet, has a neutral nature, and is non-toxic. It can quench thirst and relieve vexation, remove heat from the upper diaphragm, moisten the five internal organs, promote urination and defecation, and its effects are the same as those of honey. It is an ideal fruit for diabetic patients. Hovenia dulcis Thunb. is applicable to symptoms such as thirst in febrile diseases, drunkenness, vexatious thirst, vomiting, and fever. Modern medical research has found that the water extract of Hovenia dulcis Thunb. has the effect of activating aldehyde dehydrogenase and reducing the accumulation of acetaldehyde.

[0006] Mung bean is the seed of Vigna radiata (L.) R. Wilczek, which belongs to the legume family. It enters the heart and stomach meridians. It has the functions of clearing heat and detoxifying, relieving summer heat, promoting diuresis, replenishing qi, and detoxifying alcohol and food poisons. Dark Plum, also known as sour plum, yellow fruit, combined Chinese plum, and dry branch plum, is the nearly ripe fruit of Prunus mume Sieb. et Zucc., a deciduous arbor of the Rosaceae family, and is made by smoking with fire. Dark Plum contains more potassium and less sodium. Therefore, it is suitable for those who need to take potassium-excreting diuretics for a long time. Plum contains tannic acid which can promote intestinal peristalsis. Therefore, it is suitable for constipated people. Plum contains various organic acids and has the effect of improving liver function. Therefore, it is suitable for patients with liver diseases. The plum acid in plum can soften blood vessels, delay vascular sclerosis, and has the effect of preventing aging and anti-aging. Its properties and flavors are sour, astringent, and flat; it belongs to the liver, spleen, lung, and large intestine meridians. It has the functions of astringing the lung, astringing the intestine, promoting fluid production, and expelling roundworms. It is used for chronic cough due to lung deficiency; vexatious thirst due to deficiency heat; at the same time, it also has the functions of protecting the stomach and relieving alcoholism.

[0007] Licorice, also known as Guolao, sweet grass, Glycyrrhiza uralensis Fisch., and sweet root. It is a perennial herb of the Legume family and Glycyrrhiza genus. Its roots and rhizomes are thick and it is a kind of tonic Chinese herbal medicine. It has a faint smell and a sweet and special taste. Its functions and indications are clearing heat and detoxifying, resolving phlegm and relieving cough, and treating epigastric pain, etc. It likes a dark and humid environment and a dry climate with long sunshine and low temperature. Hong Jing said: This herb is the main ingredient of most Chinese herbal medicines, and there are few prescriptions in classical formulas that do not use it, just like agarwood in fragrant substances.

[0008] Since about 90% of traditional Chinese medicinal materials are plants, most of the medicinal active ingredients are encapsulated within the plant cell walls. The plant cell walls are composed of cellulose, hemicellulose, pectin, lignin and other substances to form a dense structure. During the process of extracting medicinal active ingredients, when the active ingredients diffuse into the extraction medium, they must overcome the double resistance of the plant cell walls and the cell interstitium. The existing traditional Chinese medicine decoction pieces and traditional Chinese medicine granules adopt traditional processing methods (such as traditional Chinese medicine decoction, maceration method, percolation method, etc.), which cannot effectively break the plant cell walls, and the active ingredients cannot fully diffuse into the medium. There are many disadvantages such as large loss of active ingredients, low yield, insufficient utilization of traditional Chinese medicine resources, many processing procedures, long cycle, high processing temperature, high energy consumption and high cost. Coupled with the shortened growth cycle of modern artificially cultivated traditional Chinese medicinal materials, the reduced content of active ingredients, and the restrictions of the pharmacopoeia on the dosage of medicinal materials, the traditional Chinese medicine cannot achieve its expected curative effect, becoming the biggest bottleneck restricting the application of traditional Chinese medicinal materials.

[0009] Traditional enzyme fermentation is a natural fermentation process, that is, it is fermented by mixed flora in the natural environment without the dominance of dominant flora and without artificial regulation of the fermentation process. Usually, the fermentation time is three months or even longer. Due to the lack of artificial inoculation and regulation work, harmful bacteria, especially molds, will infect the products during the traditional enzyme fermentation process, causing the phenomenon of excessive pathogenic microorganisms, which does not meet the requirements of food hygiene standards.

[0010] Therefore, using modern high-tech means to overcome the disadvantages in the utilization process of traditional Chinese medicine, effectively exert the medicinal effects of traditional Chinese medicinal materials, and improve economic benefits have become the key in the development and utilization of traditional Chinese medicine. Summary of the Invention

[0011] The purpose of the present invention is to provide a traditional Chinese medicine enzyme for reducing the accumulation of acetaldehyde in the body after drinking and its preparation method, that is, a food enzyme for both medicine and food for wine that can inhibit the activity of alcohol dehydrogenase, improve the activity of acetaldehyde dehydrogenase, and reduce the accumulation of acetaldehyde in the body, as well as its preparation method. At the same time, the present invention can solve the disadvantages in the utilization process of traditional Chinese medicine and effectively exert the medicinal effects of traditional Chinese medicinal materials.

[0012] To achieve the above purpose, the present invention provides the following technical solutions:

[0013] A traditional Chinese medicine enzyme for reducing the accumulation of acetaldehyde in the body after drinking, the traditional Chinese medicine enzyme is prepared from traditional Chinese medicine raw materials and auxiliary materials; the traditional Chinese medicine raw materials include wax gourd peel, kudzu root, oriental raisin tree seed, smoked plum, liquorice root, mung bean; the auxiliary material is a sweetener.

[0014] Further, the sweetener is one or more of D-mannitol, xylitol, isomaltulose, sorbitol, high maltose powder, white granulated sugar in any proportion.

[0015] Further, by weight parts, the wax gourd peel is 80 - 150 parts, the kudzu root is 80 - 150 parts, the fruit of Japanese raisin tree is 200 - 300 parts, the dark plum is 100 - 150 parts, the liquorice is 30 - 50 parts, and the mung bean is 100 - 150 parts.

[0016] The preparation method of a traditional Chinese medicine enzyme for reducing the accumulation of acetaldehyde in the body after drinking is as follows:

[0017] S1: Prepare the enzymolysis extract

[0018] Mix the traditional Chinese medicine raw materials with water, heat and cook, and cool to obtain the original liquid; add a compound enzyme for enzymolysis treatment to obtain the enzymolysis extract;

[0019] S2: Inactivate the enzyme treatment

[0020] Perform inactivating enzyme treatment on the enzymolysis extract to obtain the enzymolysis extract after inactivating enzyme treatment;

[0021] S3: Prepare the enzyme stock solution

[0022] Add a compound engineering bacterium to the enzymolysis extract after inactivating enzyme treatment for fermentation treatment, end the fermentation, and obtain the enzyme stock solution;

[0023] S4: Prepare the traditional Chinese medicine enzyme

[0024] Dilute the enzyme stock solution with water to obtain the diluted enzyme stock solution; add food - acceptable auxiliary materials and sterilize to obtain the traditional Chinese medicine enzyme.

[0025] Further, in the step S1, the traditional Chinese medicine raw materials and the water are mixed at a mass ratio of 1:10; the heating and cooking time is not less than 2 h;

[0026] The temperature after cooling is 35°C - 45°C; the conditions for the enzymolysis treatment are that the enzymolysis temperature is 35°C - 45°C; the enzymolysis time is 2.5 h - 4 h;

[0027] The material - enzyme ratio of the traditional Chinese medicine hydrated original liquid and the compound enzyme is (17 - 25):1, and the preferred material - enzyme ratio is 25:1;

[0028] The compound enzyme is one or a mixture of α - amylase, cellulase, protease, and pectinase.

[0029] Further, in the step S1, the mass ratio of α - amylase, cellulase, and pectinase in the compound enzyme is (1.2 - 1.6):1:1, and the preferred mass ratio of α - amylase, cellulase, and pectinase is 1.3:1:1.

[0030] Further, in the step S2, the inactivated enzyme treatment is any one of high-temperature enzyme inactivation, enzyme inhibitor-mediated enzyme inactivation, antibody-mediated enzyme inactivation, and protein-binding enzyme inactivation. Preferably, the inactivated enzyme treatment is an inactivated enzyme treatment method, specifically heating to 75°C and maintaining for half an hour.

[0031] Further, the specific steps of S3 are that after adjusting the pH value of the enzymolysis extract of the inactivated enzyme treatment to 5.0 - 6.0, a carbon source and the composite engineering bacteria are added for fermentation treatment;

[0032] By mass fraction, the addition amount of the carbon source is 11% - 16% of the mass of the enzymolysis extract of the inactivated enzyme treatment; the carbon source includes amino acids and saccharide substances; the mass of the amino acids is 3% - 4% of the mass of the enzymolysis extract of the inactivated enzyme treatment; the mass of the saccharide substances is 8% - 12% of the mass of the enzymolysis extract of the inactivated enzyme treatment; the amino acids are one or more of sodium glutamate, lysine, and leucine; the saccharide substances are one or more of brown sugar, fructose, and glucose; preferably, the saccharide substance is 10% brown sugar; the amino acids are 3%, and the amino acids are sodium glutamate, lysine, and leucine, with a mass ratio of 1:1:1;

[0033] The reagent for adjusting the pH is sodium bicarbonate solution, preferably adjusting the pH to 5.5;

[0034] The addition amount of the composite engineering bacteria is 0.3% - 0.7% of the mass of the enzymolysis extract of the inactivated enzyme treatment, preferably 0.5%; the composite engineering bacteria are one or more mixtures of yeast flora, acetic acid flora, and lactic acid flora;

[0035] The specific fermentation treatment is that the fermentation temperature is 25°C - 35°C, the fermentation time ≤ 5 d, and when the residual sugar content in the fermentation system ≤ 1.2 g / L, the fermentation ends to obtain the enzyme stock solution; preferably, the specific fermentation treatment is that the fermentation temperature is 30°C.

[0036] Further, by mass fraction, the composite engineering bacteria are 0.28% - 0.33% of the yeast flora, 0.10% - 0.15% of the acetic acid flora, and 0.04% - 0.08% of the lactic acid flora, and the balance is the bacterial strain preservation reagent; preferably, the yeast flora is 0.3%, the acetic acid flora is 0.12%, and the lactic acid flora is 0.08%.

[0037] Further, in the step S4, the enzyme stock solution is diluted with water until the concentration of the enzyme stock solution is (8 - 12) g / 100 mL;

[0038] The addition amount of the sweetener is 0.1% - 2% of the mass of the diluted enzyme stock solution.

[0039] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0040] 1. The present invention provides a preparation method of traditional Chinese medicine enzyme, which effectively solves the potential problems of traditional enzyme in terms of preparation time, safety and functionality. Moreover, in the organic integration of enzymatic hydrolysis treatment and fermentation process, the health care function of the enzyme is strengthened. In addition, the process of this preparation method is simple, the operation is controllable, and the fermentation time is significantly shortened.

[0041] 2. Through the compatibility of various medicinal materials, the present invention can achieve the effects of moistening the lungs and promoting the production of body fluid, promoting the production of body fluid to quench thirst, clearing heat and detoxifying, protecting the stomach, activating aldehyde dehydrogenase, and reducing the accumulation of acetaldehyde.

[0042] 3. The medicated and edible traditional Chinese medicine enzyme prepared by the present invention can significantly reduce the damage to the liver caused by free radicals generated during the alcohol metabolism process, and play the roles of protecting the liver and enhancing the hangover effect of the medicinal materials. Specific Embodiments

[0043] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without making creative efforts shall fall within the protection scope of the present invention. The experimental methods described in the following embodiments are all conventional methods unless otherwise specified; the reagents and materials described are all commercially available unless otherwise specified.

[0044] Enzymatic hydrolysis technology is a technology that uses enzymes (including cellulase, pectinase and protease) to degrade the target material, so as to completely deconstruct the target material. Traditional Chinese medicine enzymatic hydrolysis technology is to use enzyme preparations to hydrolyze medicinal plants (such as cellulase that can hydrolyze cellulose, pectinase that hydrolyzes pectin, etc.). Through enzymatic hydrolysis reaction, the components such as cellulose, hemicellulose and pectin in the cell wall and cell interstitium of traditional Chinese medicine are gently decomposed, the dense structure of the cell wall is damaged, and local loosening, swelling, collapse and other changes occur in the structure of the cell wall and cell interstitium, reducing the mass transfer resistance of the cell wall and cell interstitium and other mass transfer barriers to the diffusion of active ingredients from the cell to the extraction medium, thus being beneficial to the dissolution of active ingredients. Applied to the field of traditional Chinese medicine, it can effectively improve the extraction and separation of active ingredients of traditional Chinese medicine, increase the content of active ingredients of traditional Chinese medicine, and promote the transformation of trace active ingredients in medicine, thus promoting the technological leap of the traditional Chinese medicine industry and promoting the transformation of the traditional Chinese medicine industry into the modern traditional Chinese medicine industry. The material basis for traditional Chinese medicine to treat diseases is the effective chemical components therein. Using modern enzyme engineering technology to enzymatically hydrolyze traditional Chinese medicine can not only give full play to the efficacy components, improve the efficacy, but also produce low molecular weight cellulose to chelate and remove harmful components such as heavy metals in the medicinal materials, reducing the toxic and side effects of traditional Chinese medicine.

[0045] Enzymes are products containing specific biologically active ingredients that are obtained through microbial fermentation with or without the addition of auxiliary materials, using animals, plants, fungi, and medicinal materials as the first component. They contain various active substances, biological enzymes, and probiotics, and are rich in nutrition and high economic value. Enzyme fermentation refers to the production of a functional health drink rich in organic acids, oligosaccharides, enzymes, amino acids, vitamins, and other nutrients through multi-stage directional fermentation technology through artificial inoculation of a variety of microorganisms. The enzymatic hydrolysis products of Chinese medicinal materials that can be used as both medicine and food can be fermented by microorganisms to achieve the effect of increasing the value and extracting various active ingredients in plants, especially various plant enzymes such as polyphenols, ketones, alkenes, terpenes, polysaccharides, saponins, etc. in non-edible parts, forming more health functions.

[0046] Developing Chinese medicinal enzymes with multiple health-care functions can effectively solve the hidden dangers of traditional enzymes in terms of safety and functionality. In addition, in the organic integration of enzymatic hydrolysis and fermentation processes, enhancing the health-care functions of enzymes has become one of the technical issues that need to be urgently addressed in this field.

[0047] The present invention adopts enzymolysis technology and fermentation technology to organically combine to develop Chinese medicinal materials, which effectively solves the above technical problems.

[0048] Example 1

[0049] S1: Preparation of enzymatic extracts

[0050] The Chinese medicine raw materials were mixed with water (1:10 by mass), heated and steamed for 2 hours; cooled to 35°C to obtain the original solution; complex enzyme (material-enzyme ratio of 25:1, mass ratio) was added for enzymatic hydrolysis at 40°C for 2.5 hours to obtain the enzymatic extract.

[0051] The Chinese medicinal raw materials include wax gourd peel, kudzu root, Hovenia dulcis fruit, ebony plum, licorice, and mung bean, which include, by weight: 150 parts of wax gourd peel, 80 parts of kudzu root, 200 parts of Hovenia dulcis fruit, 150 parts of ebony plum, 30 parts of licorice, and 150 parts of mung bean.

[0052] The compound enzyme is a mixture of one or more of α-amylase, cellulase, protease and pectinase. The compound enzyme of this embodiment is a mixture of α-amylase, cellulase and pectinase in a mass ratio of 1.3:1:1.

[0053] S2: Inactivation of enzyme treatment

[0054] The enzymatic extract is subjected to an enzyme inactivation treatment to obtain an enzyme inactivated enzyme treated enzymatic extract.

[0055] Among them, the inactivated enzyme treatment is any one of high-temperature enzyme inactivation, enzyme inhibitor-mediated enzyme inactivation, antibody-mediated enzyme inactivation, and protein-binding enzyme inactivation. In this example, the inactivated enzyme treatment method is to heat to 75 °C and maintain for half an hour.

[0056] S3: Prepare the enzyme extract

[0057] In the enzymolysis extract treated with inactivated enzyme, after adjusting the pH value to 5.5, a carbon source and a composite engineering bacterium are added for fermentation treatment. The fermentation temperature is 30 °C, and the fermentation time is 3 d. When the residual sugar content in the fermentation system ≤ 1.2 g / L, the fermentation is ended to obtain the enzyme extract.

[0058] The carbon source includes amino acids and saccharide substances. The mass of amino acids is 3%-4% of the mass of the enzymolysis extract treated with inactivated enzyme; the mass of saccharide substances is 8%-12% of the mass of the enzymolysis extract treated with inactivated enzyme. The amino acids are one or more mixtures of sodium glutamate, lysine, and leucine; the saccharide substances are one or more mixtures of brown sugar, fructose, and glucose. By mass fraction, the addition amount of the carbon source is 13% of the mass of the enzymolysis extract treated with inactivated enzyme; among them, brown sugar is 10%, and amino acids are 3%; among them, the amino acids are sodium glutamate, lysine, and leucine, and the mass ratio is 1:1:1.

[0059] The reagent for adjusting pH is sodium bicarbonate solution.

[0060] The addition amount of the composite engineering bacterium is 0.3%-0.7% of the mass of the enzymolysis extract treated with inactivated enzyme. In this example, it is 0.5%. The composite engineering bacterium is a mixture of three types: yeast flora, acetic acid flora, and lactic acid flora. In this example, by mass fraction, the composite engineering bacterium is 0.3% of yeast flora, 0.12% of acetic acid flora, and 0.08% of lactic acid flora, and the balance is the bacterial strain preservation reagent.

[0061] S4: Prepare the traditional Chinese medicine enzyme

[0062] The enzyme extract is diluted with water until the concentration of the enzyme extract is 8 g / 100 mL to obtain the diluted enzyme extract; food-acceptable excipients are added and sterilized to obtain the traditional Chinese medicine enzyme. The excipient is the sweetener white granulated sugar. The addition amount of the sweetener is 1.5% of the mass of the diluted enzyme extract.

[0063] Example 2

[0064] S1: Prepare the enzymolysis extract

[0065] The traditional Chinese medicine raw materials are mixed with water (by mass 1:10), heated and steamed. The heating and steaming time is 2 h; cooled to a temperature of 35 °C to obtain the original liquid; a composite enzyme (the ratio of material to enzyme is 25:1) is added for enzymolysis treatment. The enzymolysis temperature is 35 °C, and the enzymolysis time is 4 h to obtain the enzymolysis extract.

[0066] The Chinese medicinal raw materials include, by weight: 80 parts of wax gourd peel, 150 parts of kudzu root, 300 parts of Hovenia dulcis, 120 parts of black plum, 40 parts of liquorice, and 120 parts of mung bean. The mass ratio of α-amylase, cellulase, and pectinase in the complex enzyme is 1.3:1:1.

[0067] S2: Inactivation of enzyme treatment

[0068] The enzymatic extract is subjected to an enzyme inactivation treatment to obtain an enzyme inactivated enzyme treated enzymatic extract.

[0069] The enzyme inactivation treatment method used in this embodiment is to heat to 75°C and keep it for half an hour.

[0070] S3: Preparation of enzyme stock solution

[0071] In the enzymatic extract treated with inactivated enzyme, after adjusting the pH value to 5.0, a carbon source and a composite engineered bacteria were added for fermentation treatment. The fermentation temperature was 30°C and the fermentation time was 3 days. When the residual sugar content in the fermentation system was ≤1.2 g / L, the fermentation was terminated to obtain the enzyme stock solution.

[0072] The amount of carbon source added is 13% of the mass of the enzymatic extract treated with inactivated enzymes, including 10% brown sugar and 3% amino acids, wherein the amino acids are sodium glutamate, lysine, and leucine, with a mass ratio of 1:1:1. The reagent for adjusting pH is sodium bicarbonate solution.

[0073] The amount of the composite engineering bacteria added is 0.5% of the mass of the enzymatic extract treated with the inactivated enzyme; the composite engineering bacteria is a mixture of yeast flora, acetic acid bacteria flora and lactic acid bacteria flora;

[0074] Calculated by mass fraction, the composite engineering bacteria are 0.3% of yeast bacteria, 0.12% of acetic acid bacteria and 0.08% of lactic acid bacteria, and the remainder is a bacteria preservation agent.

[0075] S4: Preparation of Chinese herbal enzymes

[0076] The enzyme stock solution is diluted with water until the concentration of the enzyme stock solution is 12g / 100mL to obtain a diluted enzyme solution; an auxiliary material acceptable to food is added, sterilized, and a Chinese medicine enzyme is obtained. The auxiliary material is a sweetener, and the sweetener is xylitol. The amount of the sweetener added is 1.0% of the mass of the diluted enzyme stock solution.

[0077] Example 3

[0078] S1: Preparation of enzymatic extracts

[0079] The traditional Chinese medicine raw materials are mixed with water (by mass ratio of 1:10), heated and steamed, and the heating and steaming time is 2 h; cooled to a temperature of 35 °C to obtain the original liquid; adding a composite enzyme (the ratio of raw material to enzyme is 25:1) for enzymatic hydrolysis treatment, the enzymatic hydrolysis temperature is 42 °C, and the enzymatic hydrolysis time is 2.5 h to obtain the enzymolysis extract.

[0080] The components of the traditional Chinese medicine raw materials by weight include: 100 parts of wax gourd peel, 100 parts of kudzu root, 250 parts of oriental raisin tree seed, 100 parts of dark plum, 50 parts of liquorice, and 100 parts of mung bean. The mass ratio of α-amylase, cellulase, and pectinase in the composite enzyme is 1.3:1:1.

[0081] S2: Inactivation of enzyme treatment

[0082] The enzymolysis extract is subjected to inactivation of enzyme treatment to obtain the enzymolysis extract after inactivation of enzyme treatment.

[0083] The inactivation of enzyme treatment method adopted in this embodiment is to heat to 75 °C and keep for half an hour.

[0084] S3: Preparation of enzyme solution

[0085] In the enzymolysis extract after inactivation of enzyme treatment, after adjusting the pH value to 5.0, adding a carbon source and a composite engineering bacteria for fermentation treatment, the fermentation temperature is 30 °C, and the fermentation time is 3 d. When the residual sugar content in the fermentation system ≤ 1.2 g / L, the fermentation is ended to obtain the enzyme solution.

[0086] The reagent for adjusting pH is sodium bicarbonate solution.

[0087] By mass fraction, the addition amount of the carbon source is 13% of the mass of the enzymolysis extract after inactivation of enzyme treatment; among them, brown sugar is 10%, and amino acids are 3%; among them, the amino acids are sodium glutamate, lysine, and leucine, and the mass ratio is 1:1:1.

[0088] The addition amount of the composite engineering bacteria is 0.5% of the mass of the enzymolysis extract after inactivation of enzyme treatment; the composite engineering bacteria are a mixture of yeast flora, acetic acid flora, and lactic acid flora; by mass fraction, the composite engineering bacteria are 0.3% of yeast flora, 0.12% of acetic acid flora, and 0.08% of lactic acid flora, and the balance is the bacterial strain preservation reagent.

[0089] S4: Preparation of traditional Chinese medicine enzyme solution

[0090] The enzyme solution is diluted with water until the concentration of the traditional Chinese medicine is 10 g / 100 mL to obtain the diluted enzyme solution; adding food-acceptable excipients and sterilizing to obtain the traditional Chinese medicine enzyme solution. The excipient is a sweetening agent, and the sweetening agent is xylitol. The addition amount of the sweetening agent is 1.2% of the mass of the diluted enzyme solution.

[0091] Application example

[0092] Forty-eight volunteers were organized. After taking 300 ml of the medicinal and edible Chinese herbal medicine enzyme that can reduce the accumulation of acetaldehyde in the body after drinking (the control group took 300 ml of water), they immediately started to drink Moutai Chun wine (53 degrees), and drank 100 ml of wine within half an hour. 2.5 ml of peripheral blood was taken 2 hours after starting to drink, anticoagulated with sodium citrate, centrifuged at 300 g for 10 minutes, the plasma was separated, and the plasma acetaldehyde concentration was measured. Take 0.1 ml of plasma, add 4.6 ml of pure water, then add 0.3 ml of 20% trichloroacetic acid, shake and centrifuge at 4000 r / min for 10 min. Take the supernatant and filter it through a 0.45 nm filter membrane for gas chromatography determination. Chromatographic conditions: GCl22 type gas chromatograph (Shanghai Precision Scientific Instruments Co., Ltd.), N2000 chromatographic data workstation; quartz capillary column (PEG-20000, 30 m × 2.5 mm × 0.5 μm); hydrogen flame ionization detector; column temperature 70 °C, vaporization chamber 150 °C, detection chamber 180 °C; carrier gas: nitrogen, column front pressure 0.12 MPa, diaphragm cleaning needle valve adjusted to 1, split needle valve scale adjusted to 3, tail blowing steady flow valve scale adjusted to 3; hydrogen pressure 0.12 MPa, steady flow valve scale adjusted to 5; air pressure 0.15 MPa, steady flow valve scale adjusted to 6; sensitivity 1010; attenuation 1. The plasma acetaldehyde concentration measurement results are shown in Table 1: Compared with the control group, the plasma acetaldehyde concentration in each group was significantly reduced (P<0.05).

[0093] Table 1. Measurement results of acetaldehyde content in plasma

[0094]

[0095]

[0096] *Significantly different from the control group (P<0.05)

[0097] As shown in Table 1, compared with the control group, the plasma acetaldehyde content in the three medicinal and edible Chinese herbal medicine enzyme groups was significantly reduced, and the difference was significant (P<0.05). Among them, the subjects in the group taking Example 2 had the greatest degree of reduction, presumably related to the highest enzyme content in the group of Example 2. Generally speaking, the medicinal and edible Chinese herbal medicine enzyme, through the compatibility of various medicinal materials, has the effects of moistening the lungs and promoting fluid production, clearing heat and detoxifying, protecting the stomach, activating acetaldehyde dehydrogenase, and reducing the accumulation of acetaldehyde; at the same time, it can significantly reduce the damage of free radicals generated during alcohol metabolism to the liver, playing the role of protecting the liver and enhancing the hangover effect of medicinal materials.

[0098] The preparation method of the Chinese herbal medicine enzyme for both medicine and food solves the potential problems of traditional enzymes in terms of preparation time, safety and functionality, and strengthens the health care function of enzymes in the organic integration of enzymatic hydrolysis treatment and fermentation process. The preparation method of the present invention is controllable, simple in operation, significantly shortens the fermentation time, thus saving time and reducing production costs.

[0099] Finally, it should be noted that the above are only the preferred embodiments of the present invention and are not intended to limit the present invention. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments, or perform equivalent replacements for some of the technical features. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A traditional Chinese medicine enzyme for reducing the accumulation of acetaldehyde in the body after drinking, characterized in that: The traditional Chinese medicine enzyme is prepared from traditional Chinese medicine raw materials and auxiliary materials; The traditional Chinese medicine raw materials include wax gourd peel, kudzu root, oriental raisin tree seed, smoked plum, liquorice, and mung bean; The auxiliary material is a sweetening agent.

2. The Chinese medicine enzyme for reducing the accumulation of acetaldehyde in the body after drinking according to claim 1, wherein: The sweetening agent is one or more of D-mannitol, xylitol, isomaltitol, sorbitol, high maltose powder, and granulated sugar, mixed in any proportion.

3. A traditional Chinese medicine enzyme for reducing the accumulation of acetaldehyde in the body after drinking, as described in claim 1, characterized in that: Calculated by weight parts of components, the wax gourd peel is 80 - 150 parts, the kudzu root is 80 - 150 parts, the oriental raisin tree seed is 200 - 300 parts, the smoked plum is 100 - 150 parts, the liquorice is 30 - 50 parts, and the mung bean is 100 - 150 parts.

4. The preparation method of a traditional Chinese medicine enzyme for reducing the accumulation of acetaldehyde in the body after drinking, as described in claim 1, is characterized in that: The preparation method of the traditional Chinese medicine enzyme is as follows: S1: Prepare the enzymolysis extract The traditional Chinese medicine raw materials are mixed with water, heated and steamed, cooled to obtain the original liquid; add a compound enzyme for enzymolysis treatment to obtain the enzymolysis extract; S2: Inactivate the enzyme treatment Perform inactivating enzyme treatment on the enzymolysis extract to obtain the enzymolysis extract after inactivating enzyme treatment; S3: Prepare the enzyme stock solution Add a compound engineering bacterium to the enzymolysis extract after inactivating enzyme treatment for fermentation treatment, end the fermentation, and obtain the enzyme stock solution; S4: Prepare the traditional Chinese medicine enzyme After diluting the enzyme stock solution with water, obtain the diluted enzyme stock solution; add food-acceptable auxiliary materials and sterilize to obtain the traditional Chinese medicine enzyme.

5. The preparation method of a traditional Chinese medicine enzyme for reducing the accumulation of acetaldehyde in the body after drinking according to claim 4, characterized in that: In the step S1, the traditional Chinese medicine raw materials and the water are mixed at a mass ratio of 1:10; the heating and steaming time is not less than 2h; The temperature after cooling is 35°C - 45°C; the conditions for the enzymolysis treatment are that the enzymolysis temperature is 35°C - 45°C and the enzymolysis time is 2.5h - 4h; The material-enzyme ratio of the traditional Chinese medicine hydrated original liquid and the compound enzyme is (17 - 25):1; The compound enzyme is a mixture of one or more of α-amylase, cellulase, protease, and pectinase.

6. The preparation method of a traditional Chinese medicine enzyme for reducing the accumulation of acetaldehyde in the body after drinking according to claim 5, characterized in that: In the step S1, the mass ratio of α-amylase, cellulase, and pectinase in the compound enzyme is (1.2 - 1.6):1:

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7. A preparation method of a traditional Chinese medicine enzyme for reducing the accumulation of acetaldehyde in the body after drinking, characterized in that: In the step S2, the inactivating enzyme treatment is any one of high-temperature enzyme inactivation, enzyme inhibitor enzyme inactivation, antibody-mediated enzyme inactivation, and protein-binding enzyme inactivation.

8. The preparation method of a traditional Chinese medicine enzyme for reducing the accumulation of acetaldehyde in the body after drinking according to claim 4, characterized in that: The specific steps of S3 are that after adjusting the pH value of the enzymolysis extract after inactivating enzyme treatment to 5.0 - 6.0, add a carbon source and the compound engineering bacterium for fermentation treatment; Calculated by mass fraction, the addition amount of the carbon source is 11% - 16% of the mass of the enzymolysis extract after inactivating enzyme treatment; the carbon source includes amino acids and saccharide substances; the mass of the amino acids is 3% - 4% of the mass of the enzymolysis extract after inactivating enzyme treatment; the mass of the saccharide substances is 8% - 12% of the mass of the enzymolysis extract after inactivating enzyme treatment; the amino acids are a mixture of one or more of sodium glutamate, lysine, and leucine; the saccharide substances are a mixture of one or more of brown sugar, fructose, and glucose; The reagent for adjusting the pH is a sodium bicarbonate solution; The addition amount of the composite engineered bacteria is 0.3%-0.7% of the mass of the enzymolysis extract treated with the inactivated enzyme; the composite engineered bacteria are one or a mixture of yeast flora, acetic acid flora and lactic acid flora; The fermentation treatment specifically is that the fermentation temperature is 25°C-35°C, the fermentation time is ≤5 days, and when the residual sugar content in the fermentation system is ≤1.2 g / L, the fermentation is ended to obtain the enzyme stock solution.

9. The preparation method of a traditional Chinese medicine enzyme for reducing the accumulation of acetaldehyde in the body after drinking, as claimed in claim 8, is characterized in that: By mass fraction, the composite engineered bacteria are 0.28%-0.33% of the yeast flora, 0.10%-0.15% of the acetic acid flora and 0.04%-0.08% of the lactic acid flora, and the balance is the bacterial strain preservation reagent.

10. A preparation method of a traditional Chinese medicine enzyme for reducing the accumulation of acetaldehyde in the body after drinking, characterized in that: In the step S4, the enzyme stock solution is diluted with water until the concentration of the enzyme stock solution is (8-12) g / 100 mL; The addition amount of the sweetener is 0.1%-2% of the mass of the diluted enzyme stock solution.

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