Method for improving yield of valarene by using saccharomyces cerevisiae rearrangement technology
Through synthetic chromosomal rearrangement technology and yeast Mating mating, a diploid valenne production strain was constructed, which solved the problem of low valenne production in Saccharomyces cerevisiae haploid strain, and achieved a significant increase in valenne production.
CN120349906APending Publication Date: 2025-07-22TIANJIN UNIV
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Patent Information
- Application Number
- CN202510338866.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-03-21
- Publication Date
- 2025-07-22
AI Technical Summary
Technical Problem
In the prior art, the Saccharomyces cerevisiae haploid strain is less efficient in valenne production, and there is no effective rearrangement technology to improve valenne production.
Method used
The Saccharomyces cerevisiae rearrangement technology was used to construct a library of Saccharomyces cerevisiae rearrangement strains, and the haploid strains were mated with valenne haploid production strains through yeast Mating technology to generate diploid valenne production strains, achieving genomic evolution.
Benefits of technology
Through the fermentation of diploid valenone production strains, the valenone production increased by 18.5%, reaching 194.14 mg/L, significantly improving the production capacity of Saccharomyces cerevisiae.
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Abstract
The invention belongs to the technical field of biosynthesis, and discloses a method for improving the yield of valarene by using a saccharomyces cerevisiae rearrangement technology, which comprises the following steps: firstly, constructing a saccharomyces cerevisiae rearrangement strain library by using a synthetic chromosome rearrangement technology; then, the haploid strains in the saccharomyces cerevisiae rearrangement strain library and the valarene haploid production strains are mated to generate diploid valarene production strains; and finally, fermenting the diploid valarene production strain to obtain a valarene product. Fermentation verification is carried out on a diploid ceraene production strain and a haploid ceraene production strain (contrast), and results show that the yield of the ceraene haploid production strain is 163.81 mg / L, the highest yield of the diploid production strain is 194.14 mg / L, the yield is improved by 18.5% compared with that of an original strain, and it is proved that the production capacity of the ceraene is improved through the method.
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