Microsatellite marker of temperature regulation related functional gene of eriocheir sinensis, detection method, kit, analysis method, screening method and breeding method

By using bioinformatics screening and specific primer design, combined with capillary electrophoresis separation, the problem of insufficient specificity in the amplification of temperature regulation-related functional gene markers in mud crabs has been solved in existing technologies. This has enabled efficient polymorphism analysis and molecular marker-assisted breeding, which has important applications, especially in the breeding of superior temperature-resistant varieties.

CN120350141BActive Publication Date: 2026-06-02RIZHAO OCEAN & FISHERY RES INST (RIZHAO SEA AREA USAGE DYNAMIC MONITORING & MONITORING CENT RIZHAO AQUATIC WILDLIFE RESCUE STATION) +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
RIZHAO OCEAN & FISHERY RES INST (RIZHAO SEA AREA USAGE DYNAMIC MONITORING & MONITORING CENT RIZHAO AQUATIC WILDLIFE RESCUE STATION)
Filing Date
2025-06-19
Publication Date
2026-06-02

AI Technical Summary

Technical Problem

Existing technologies lack specificity in amplifying microsatellite markers for temperature regulation-related functional genes in mud crabs, failing to establish stable associations with temperature-controlled phenotypes. This results in non-specific bands and primer dimer interference in the context of highly complex genomes, making them unsuitable for direct use in molecular marker-assisted breeding of temperature regulation-related functional genes in mud crabs.

Method used

Bioinformatics analysis was used to screen temperature regulation-related functional genes, specific primers were designed for PCR amplification, and capillary electrophoresis was used for separation. Seven highly polymorphic microsatellite markers were screened, and fluorescent markers were used for genotyping. A microsatellite marker detection method for temperature regulation-related functional genes in mud crab was established.

Benefits of technology

This study enabled efficient detection and polymorphism analysis of temperature regulation-related functional genes in mud crabs, providing important molecular markers for population genetic structure analysis and marker-assisted breeding, and has significant application value, especially in the breeding of superior temperature-resistant varieties.

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Abstract

The present application belongs to the technical field of biological information analysis, and particularly relates to a microsatellite marker of a temperature regulation related functional gene of Scylla paramamosain, a detection method, a kit, an analysis method, a screening method and a breeding method. Through transcriptome data analysis, the present application screens a temperature adaptability related functional gene, develops a microsatellite marker of the temperature regulation related functional gene of Scylla paramamosain, and provides polymorphic primers, establishes a technical system and a detection method for development of the microsatellite marker of the functional gene of Scylla paramamosain. The present application not only lays a foundation for Scylla paramamosain genetic diversity research, genetic map construction and evolution analysis, but also provides valuable and reliable molecular markers for breeding of fine varieties of Scylla paramamosain.
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