Instant whitening composition and application thereof

Abstract

The invention relates to the technical field of daily chemicals, in particular to an instant whitening composition and application thereof. The whitening composition is prepared from saccharomycetes / rice fermentation product filtrate, a lactobacillus fermentation product, rosa damascena flower water, butanediol, p-hydroxyacetophenone and 1, 2-hexanediol, the invention provides a scientific compounding scheme of three core fermentation components for the first time, namely the saccharomycetes / rice fermentation product filtrate and the lactobacillus fermentation product, and the lactobacillus fermentation product is mild and exfoliating, and promotes penetration of active peptide in the saccharomycetes fermentation liquid, so that the skin is immediately bright and white. The rosa damascena flower water is rich in flavonoids, and cooperates with glutathione of the yeast product, so that the oxidation resistance is enhanced, and the melanin generation is delayed. The lactobacillus fermentation product, the rosa damascena water and the lactobacillus metabolite can regulate the micro-ecology of the skin, and the rosa damascena water can inhibit inflammation, repair a barrier together and improve the glossiness of the skin.

Description

Technical Field

[0001] The present invention relates to the field of daily chemical technology, and particularly relates to an instant whitening composition and its application. Background Art

[0002] At present, most of the whitening skin care products on the market use chemical components (such as arbutin, niacinamide) as the main active ingredients, but these chemical components may cause irritation to sensitive skin or pose potential safety hazards after long-term use. Therefore, how to use natural fermentation components to achieve instant whitening while maintaining mildness and high efficiency has become an important direction in the research and development of skin care products.

[0003] The present invention combines three natural fermentation products, synergistically promotes melanin metabolism, improves skin brightness, strengthens the skin barrier, and adopts a unique biological fermentation and extraction process to make the active substances stable and have a high absorption rate, so as to achieve the dual effects of instant whitening and long-lasting whitening. Summary of the Invention

[0004] To solve the above technical problems, the present invention provides an instant whitening composition, which comprises: filtrate of Saccharomyces cerevisiae / rice fermentation product, Lactobacillus fermentation product, Rosa damascena flower water, butylene glycol, p-hydroxyacetophenone and 1,2-hexanediol.

[0005] As an embodiment of the present invention, the mass ratio of the filtrate of Saccharomyces cerevisiae / rice fermentation product, Lactobacillus fermentation product, Rosa damascena flower water, butylene glycol, p-hydroxyacetophenone and 1,2-hexanediol is: (40-60):(5-35):0.5:0.5.

[0006] As an embodiment of the present invention, the mass ratio of the filtrate of Saccharomyces cerevisiae / rice fermentation product, Lactobacillus fermentation product, Rosa damascena flower water, butylene glycol, p-hydroxyacetophenone and 1,2-hexanediol is: 49:15:0.5:0.5.

[0007] As an embodiment of the present invention, the preparation method of the filtrate of Saccharomyces cerevisiae / rice fermentation product is as follows:

[0008] (2) Select brown rice, soak it in 3 times the volume of pure water for 12 hours, after high-pressure steaming for 30 minutes, add 0.5% w / v α-amylase and keep it at 65 °C for 2 hours, and then cool it to below 30 °C;

[0009] (2) Yeast culture: In a medium containing nitrogen source, glucose and trace elements, add the brown rice cooled in step (1), and at the same time inoculate 10 6 CFU / mL of yeast, and carry out aerobic fermentation at 28 °C for 48 hours;

[0010] (3) Low-temperature extraction: After fermentation is completed, the supernatant is collected by centrifugation, and the filtrate is obtained by low-temperature membrane filtration; the filtrate of the yeast / rice fermentation product is obtained by low-temperature vacuum concentration technology.

[0011] As an embodiment of the present invention, the components of the culture medium in the step (2) are: wolfberry extract 5-10 g / L, glucose 5-15 g / L, yeast extract 5-10 g / L, peptone 3-8 g / L, ammonium sulfate 2-5 g / L, potassium dihydrogen phosphate 1-3 g / L, magnesium sulfate heptahydrate 0.5-1.5 g / L, L-cysteine 0.1-0.5 g / L, vitamin B1 0.01-0.5 g / L, ZnSO4 0.005-0.01 g / L, and water is made up to 1 L.

[0012] As an embodiment of the present invention, the preparation methods of the Lactobacillus fermentation product and the Rosa damascena flower water are as follows

[0013] S01 Prepare the culture medium:

[0014] Glucose 20-30 g / L, sucrose 10-15 g / L, yeast extract 5-10 g / L, peptone 3-5 g / L, Rosa damascena flower water 10-15 g / L, potassium dihydrogen phosphate 1-2 g / L, magnesium sulfate 0.2-0.5 g / L, vitamin B1 0.02 g / L, biotin 0.01 g / L, sodium citrate 2-4 g / L;

[0015] S02 Strain activation:

[0016] Select the frozen-preserved Lactobacillus seeds and inoculate them into the MRS liquid medium containing 10% glucose, and culture them statically at 37°C for 18 hours; observe that the turbidity of the bacterial liquid reaches 0.5-0.8 OD600;

[0017] S03 Inoculate the activated bacterial liquid into the fermenter at a ratio of 5-10 v / v%; ferment aerobically at 37-42°C for 48-72 hours, then terminate the fermentation, cool the culture medium to 4°C to inhibit the metabolism of the bacteria; then centrifuge at 6000 rpm for 15 minutes to remove the bacteria, collect the supernatant; use 0.45 μm microfiltration to further remove cell debris.

[0018] As an embodiment of the present invention, the preparation method of the Rosa damascena flower water is as follows:

[0019] Select the petals of Rosa damascena Mill., dry them in hot air at 40 - 50 °C, and crush them to 50 - 100 mesh; add an ethanol - glycerol solvent at a ratio of 1:10 w / v; extract at 60 °C with a stirring speed of 100 rpm for 2 hours; then add 0.5% w / v pectinase and 0.3% w / v cellulase and extract at 50 °C for 1 hour; then filter using a 0.45 μm microfiltration membrane and concentrate to 1 / 4 - 1 / 5 of the original volume at a temperature below 50 °C and -0.08 MPa.

[0020] As an embodiment of the present invention, the instant whitening composition is used in the field of daily chemical products.

[0021] As an embodiment of the present invention, an instant whitening facial mask contains the instant whitening composition.

[0022] As an embodiment of the present invention, the raw materials for preparing the instant whitening facial mask include:

[0023] Component A: deionized water, butylene glycol, glycerol, allantoin, carbomer 940, 1.3 million HA, p - hydroxyacetophenone, xanthan gum;

[0024] Component B: PC 2000;

[0025] Component C: 1,2 - hexanediol, compound plant anti - allergen, Portulaca oleracea extract, the instant whitening composition.

[0026] Adopting the above - mentioned technical solution, the present invention has the following beneficial effects:

[0027] The present invention first proposes a scientific compounding scheme of three core fermentation components:

[0028] 1. Yeast / Rice Ferment Filtrate, Lactobacillus Ferment: The Lactobacillus Ferment gently exfoliates, promotes the penetration of active peptides in the yeast fermentation broth, and makes the skin instantly brighten.

[0029] 2. Yeast / Rice Ferment Filtrate, Rosa damascena Mill. Water: Rosa damascena Mill. Water is rich in flavonoids, which synergize with the glutathione of the yeast product to enhance antioxidant activity and delay melanin production.

[0030] 3. Lactobacillus Ferment, Rosa damascena Mill. Water: The metabolites of lactic acid bacteria regulate the skin micro - ecosystem, and Rosa damascena Mill. Water inhibits inflammation, jointly repairing the barrier and improving the skin glossiness. Specific Embodiments

[0031] The following further explains and illustrates the present invention in combination with specific embodiments.

[0032] Example 1

[0033] This embodiment provides an instant whitening mask. By mass, its preparation raw materials include:

[0034] Component A: 76.51 parts of deionized water, 5 parts of butanediol, 5 parts of glycerin, 0.2 part of allantoin, 0.08 part of carbomer 940, 0.03 part of 1.3 million HA, 0.5 part of p-hydroxyacetophenone, 0.23 part of xanthan gum;

[0035] Component B: 0.05 part of PC 2000;

[0036] Component C: 0.6 part of 1,2 - hexanediol, 0.3 part of compound plant anti - allergy agent, 1.5 parts of purslane extract, 10 parts of the instant whitening composition.

[0037] Among them, the instant whitening composition in this embodiment includes: 49% of yeast / rice fermentation product filtrate, 15% of butanediol, 0.5% of p - hydroxyacetophenone, and 0.5% of 1,2 - hexanediol. The lactic acid bacteria fermentation product and damask rose flower water are supplemented to 100%.

[0038] Among them, the preparation method of the yeast / rice fermentation product filtrate is as follows:

[0039] (3) Select brown rice, soak it in 3 times the volume of pure water for 12 hours, after high - pressure steaming for 30 minutes, add 0.5% w / v α - amylase and keep it at 65 °C for 2 hours, then cool it to below 30 °C;

[0040] (2) Yeast culture: In the culture medium, add the brown rice cooled in step (1), and at the same time inoculate 10 6 CFU / mL of yeast, and carry out aerobic fermentation at 28 °C for 48 hours; the mass ratio of brown rice to the culture medium in step (1) is 1:5.

[0041] (3) Low - temperature extraction: After fermentation, centrifuge to collect the supernatant, and use low - temperature membrane filtration to obtain the filtrate; use the low - temperature vacuum concentration technology to obtain the yeast / rice fermentation product filtrate, and concentrate the volume to 1 / 3 of the original. The yeast is Saccharomyces cerevisiae 1388.

[0042] The components of the culture medium in step (2) are: 8 g / L of wolfberry extract, 8 g / L of glucose, 10 g / L of yeast extract, 5 g / L of peptone, 3 g / L of ammonium sulfate, 2 g / L of potassium dihydrogen phosphate, 1.0 g / L of magnesium sulfate heptahydrate, 0.3 g / L of L - cysteine, 0.2 g / L of vitamin B1, 0.01 g / L of ZnSO4, and make up the water to 1 L.

[0043] The preparation methods of the lactic acid bacteria fermentation product and damask rose flower water are as follows

[0044] S01 Preparation of culture medium:

[0045] Glucose 25 g / L, sucrose 12 g / L, yeast extract 8 g / L, peptone 4 g / L, Rosa damascena flower water 15 g / L, potassium dihydrogen phosphate 1 g / L, magnesium sulfate 0.5 g / L, vitamin B1 0.02 g / L, biotin 0.01 g / L, sodium citrate 3 g / L;

[0046] S02 Activation of bacterial strain:

[0047] Select the cryopreserved Lactobacillus seeds and inoculate them into MRS liquid medium containing 10% glucose, and incubate statically at 37 °C for 18 hours; observe that the turbidity of the bacterial liquid reaches 0.5 - 0.8 OD600; (Lactiplantibacillus plantarum 23941) 23941)

[0048] Inoculate the activated bacterial liquid into the fermenter at a ratio of 8 v / v%; after aerobic fermentation at 38 °C for 72 hours, terminate the fermentation, cool the culture medium to 4 °C to inhibit the metabolism of the bacteria; then centrifuge at 6000 rpm for 15 min to remove the bacteria, and collect the supernatant; use a 0.45 μm microfiltration to further remove cell debris.

[0049] The preparation method of the Rosa damascena flower water is as follows:

[0050] Select Rosa damascena flower petals, dry them in hot air at 40 °C, and crush them to 100 meshes; add an ethanol - glycerol solvent (mass ratio of ethanol, glycerol, and water is 10:20:70) at a ratio of 1:10 w / v; extract at 60 °C with a stirring speed of 100 rpm for 2 hours; then add 0.5% w / v pectinase and 0.3% w / v cellulase and extract at 50 °C for 1 hour; then filter using a 0.45 μm microfiltration membrane and concentrate to 1 / 4 of the original volume at a temperature below 50 °C and -0.08 MPa.

[0051] The composite plant anti - allergy agent contains: butylene glycol 49%, Avena sativa extract 55, water 38.4%, Glycyrrhiza uralensis root extract 4%, Sophora flavescens root extract 1.5%, Lonicera japonica flower extract 0.1%, Aloe barbadensis leaf juice 2%.

[0052] In this example, the preparation method of the instant whitening facial mask is as follows:

[0053] (1) Mix water, carbomer, and xanthan gum in component A evenly, then add butylene glycol, and subsequently add the components of the remaining component A. Heat to 85°C and stir evenly, then cool down to 40°C.

[0054] (2) After mixing component B and component C evenly, slowly add them to component A obtained in step (1) and stir evenly.

[0055] Example 2

[0056] The difference from Example 1 is that the instant whitening composition in this example contains: yeast / rice fermentation product filtrate 49%, butylene glycol 15%, p-hydroxyacetophenone 0.5%, and 1,2-hexanediol 0.5%, and the lactobacillus fermentation product is supplemented to 100%.

[0057] Example 3

[0058] The difference from Example 1 is that the instant whitening composition in this example contains: butylene glycol 15%, p-hydroxyacetophenone 0.5%, and 1,2-hexanediol 0.5%, and the lactobacillus fermentation product and damask rose flower water are supplemented to 100%.

[0059] Example 4

[0060] The difference from Example 1 is that the preparation method of the yeast / rice fermentation product filtrate in this example is as follows:

[0061] (4) Select brown rice, soak it in 3 times the volume of pure water for 12 hours, after high-pressure steaming for 30 minutes, add 0.5% w / v α-amylase and keep it at 65°C for 2 hours, and then cool it to below 30°C;

[0062] (2) Yeast culture: In the culture medium, add the brown rice cooled in step (1), and at the same time inoculate 10 6 CFU / mL yeast, and ferment aerobically at 28°C for 48 hours; the mass ratio of brown rice to the culture medium in step (1) is 1:5.

[0063] (3) Low-temperature extraction: After fermentation is completed, centrifuge to collect the supernatant, and use low-temperature membrane filtration to obtain the filtrate; use the low-temperature vacuum concentration technology to obtain the yeast / rice fermentation product filtrate, and concentrate the volume to 1 / 3 of the original. The yeast is Saccharomyces cerevisiae 1388.

[0064] In step (2), the medium components are: glucose 8 g / L, yeast extract 10 g / L, peptone 5 g / L, ammonium sulfate 3 g / L, potassium dihydrogen phosphate 2 g / L, magnesium sulfate heptahydrate 1.0 g / L, L-cysteine 0.3 g / L, vitamin B1 0.2 g / L, ZnSO4 0.01 g / L, and water is made up to 1 L.

[0065] Example 5

[0066] The difference from Example 1 is that in this example, the Lactobacillus fermentation product and the Rosa damascena flower water are prepared separately, that is:

[0067] The preparation method of the Lactobacillus fermentation product is as follows

[0068] S01 Prepare the medium:

[0069] Glucose 25 g / L, sucrose 12 g / L, yeast extract 8 g / L, peptone 4 g / L, potassium dihydrogen phosphate 1 g / L, magnesium sulfate 0.5 g / L, vitamin B1 0.02 g / L, biotin 0.01 g / L, sodium citrate 3 g / L; S02 Activate the strain:

[0070] Select the cryopreserved Lactobacillus seeds and inoculate them into the MRS liquid medium containing 10% glucose, and incubate statically at 37°C for 18 hours; observe that the turbidity of the bacterial liquid reaches 0.5 - 0.8 OD600; (Lactiplantibacillus plantarum 23941)

[0071] S03 Inoculate the activated bacterial liquid into the fermenter at a ratio of 8 v / v%; after aerobic fermentation at 38°C for 72 hours, terminate the fermentation, cool the medium to 4°C to inhibit the metabolism of the bacteria; then centrifuge at 6000 rpm for 15 min to remove the bacteria, and collect the supernatant; use a 0.45 μm microfiltration to further remove cell debris.

[0072] The preparation method of the Rosa damascena flower water is as follows:

[0073] Select Rosa damascena flower petals, dry them in hot air at 40°C, and crush them to 100 mesh; add an ethanol-glycerol solvent (mass ratio of ethanol, glycerol, and water is 10:20:70) at a ratio of 1:10 w / v; extract at 60°C and a stirring speed of 100 rpm for 2 hours; then add 0.5% w / v pectinase and 0.3% w / v cellulase and extract at 50°C for 1 hour; then filter using a 0.45 μm microfiltration membrane and concentrate to 1 / 4 of the original volume at a temperature below 50°C and -0.08 MPa.

[0074] Example 6

[0075] The difference from Example 1 is that in this example, the preparation method of the instant whitening mask is as follows:

[0076] (1) After mixing all the components in Component A together, heat to 85°C and then stir evenly and cool down to 40°C;

[0077] (2) Add Component B and Component C to Component A obtained in step (1), and stir evenly.

[0078] Performance Test

[0079] Test 1: Place the masks prepared in Examples 1-6 at 0°C, 25°C, and 40°C for 90 days, and check the product properties. The test results are shown in Table 1.

[0080] Thermal cycling experiment: Put the masks prepared in Examples 1-6 into a sealed container to ensure it is completely sealed to prevent interference from external humidity or gas. Place the samples in an incubator set at 45°C for 12 hours. After taking out the samples, quickly transfer them to a low-temperature box at -5°C and keep them for 12 hours. Repeat 6 times after completing 1 high-low temperature cycle; check the physical properties such as the appearance, color, odor, viscosity, layering, and particle precipitation of the samples after the cycle.

[0081] Table 1 Test results of Test 1

[0082]

[0083]

[0084] Test 2: Tyrosinase inhibition rate test:

[0085] Using L-DOPA as the substrate (1 mg / mL) and kojic acid as the positive control. Using a 96-well plate as the carrier, the experiment is divided into 4 groups: reaction group, reaction control group, blank group, and blank control group. The reaction group and the reaction control group are respectively added with 25 μL of samples with different concentrations, and the blank group and the blank control group are respectively added with 25 μL of phosphate buffer solution. Subsequently, 25 μL of tyrosinase is added to the reaction group and the blank group, while the reaction control group and the blank control group replace tyrosinase with an equal amount of PBS, mix evenly, incubate at 37°C for 5 min, then add 100 μL of L-DOPA solution to each well, continue to react in a 37°C incubator for 5 min, and then measure the absorbance (A) value at a wavelength of 475 nm. The experimental data are all the averages after 3 repetitions. Calculate the tyrosinase inhibition rate according to the following formula, and calculate the IC50 value using SPSS software.

[0086] Inhibition rate = 1 - (A 反应 - A 反应对照 ) / (A 空白 - A 空白对照 )

[0087] Test results of performance test 3 (n = 3)

[0088]

[0089]

[0090] Hyaluronidase inhibition experiment

[0091] Detection basis: Test according to the hyaluronidase inhibition rate of HMC-W1-029

[0092] Examples 1-6: Dilute with pure water to a sample concentration of 0.2%;

[0093] Positive control (dipotassium glycyrrhizinate, purity ≥ 98%): Dilute with water to a positive control concentration of 3%;

[0094] Negative control: Pure water

[0095] Test operation steps:

[0096] Design the experimental group, positive control group, and negative control group. Each group needs to set up 3 parallels. Add different reagent solutions respectively, shake well, place at room temperature for 30 min to develop color, and measure the absorbance value at a wavelength of 528 nm with an ultraviolet spectrophotometer.

[0097] Calculation formula

[0098] Hyaluronidase inhibition rate (%) = (1 - (C - D) / (A - B)) × 100

[0099] Where, A is the absorbance of the reaction solution without the sample; B is the absorbance of the reaction solution without the sample and enzyme; C is the absorbance of the reaction solution containing the sample and enzyme; D is the absorbance of the reaction solution containing the sample and without enzyme.

[0100] The statistical analysis software is SPSS. The comparison of the hyaluronidase inhibition rates among the test samples, positive control, and negative control is performed using an independent sample t-test. The above statistical analyses are all two-tailed tests, with a significance level of α = 0.05. P > 0.05 indicates no significant difference between the two groups; P < 0.05 indicates a significant difference between the two groups.

[0101] Table 3 Test result table

[0102] Name Experimental results P value Example 1 71.356±0.236 ＜0.05 Example 2 59.284±0.174 ＜0.05 Example 3 60.184±0.427 ＜0.05 Example 4 64.294±0.525 ＜0.05 Example 5 66.296±0.925 ＜0.05 Example 6 68.638±0.274 ＜0.05 Negative control -4.618±0.110 / Positive control 50.384±1.732 ＜0.05

[0103] The hyaluronidase inhibition rate is retained to three decimal places. P < 0.05 indicates a significant difference between the sample and the positive control compared with the negative control.

[0104] The present invention combines three natural fermentation products to synergistically promote melanin metabolism, enhance skin brightness, and strengthen the skin barrier. By adopting a unique biological fermentation and extraction process, the active substances are stable and have a high absorption rate, thus achieving the dual effects of instant whitening and long-term whitening. Thickeners and humectants may form an unstable gel network at different pH values. If the pH is not properly adjusted or some components interact with electrolytes, it may lead to the disintegration, precipitation, or uneven dispersion of the gel structure. Ingredients such as yeast / rice fermentation filtrate and lactobacillus fermentation products contain proteins, polysaccharides, and microbial metabolites, which may interact with cations or high-molecular-weight thickeners (such as carbomer), resulting in precipitation. In addition to adjusting the preparation methods of the effective components of the three during preparation, when preparing the facial mask liquid, it is mixed in stages. After dissolving the thickener in component A first, component B is added, and finally component C is added to avoid mutual influence between components.

[0105] Test 4: The products prepared in Examples 1 to 6 were subjected to an instant whitening questionnaire test. A total of 32 people were selected, including 7 males and 25 females, aged 25 to 55 years, with an average age of 32.1 ± 2.1 years, meeting the volunteer selection criteria for subjects.

[0106] The content of the questionnaire is shown in Table 3.

[0107] Table 3 Content of the Questionnaire for Subjects of Instant Whitening

[0108]

[0109] 5 points indicate full agreement, 4 points indicate relatively agreement, 3 points indicate neither agreement nor disagreement (i.e., average), 2 points indicate relatively disagreement, and 1 point indicates full disagreement. The results are the numerical aggregates of 32 people.

[0110] Table 4 Results of the Questionnaire for Subjects of Sunscreen Removal

[0111] Example Problem 1 Problem 2 Problem 3 Problem 4 Problem 5 1 160 158 155 156 157 2 158 141 122 120 138 3 169 146 124 122 140 4 158 149 136 133 142 5 158 152 140 140 150 6 159 156 142 145 153

[0112] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that they can still modify the technical solutions described in the foregoing embodiments, or perform equivalent replacements on some or all of the technical features; and these modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. An instant whitening composition, characterized in that, The whitening composition comprises: yeast / rice ferment filtrate, lactobacillus ferment, rose damascena flower water, butylene glycol, p-hydroxyacetophenone and 1,2-hexanediol; The preparation methods of the lactobacillus ferment and the rose damascena flower water are as follows S01 Prepare the medium: Glucose 20 - 30 g / L, sucrose 10 - 15 g / L, yeast extract 5 - 10 g / L, peptone 3 - 5 g / L, rose damascena flower water 10 - 15 g / L, potassium dihydrogen phosphate 1 - 2 g / L, magnesium sulfate 0.2 - 0.5 g / L, vitamin B1 0.02 g / L, biotin 0.01 g / L, sodium citrate 2 - 4 g / L; S02 Activate the strain: Select the cryopreserved lactobacillus seeds and inoculate them into the MRS liquid medium containing 10% glucose, and statically culture at 37°C for 18 hours; observe that the turbidity of the bacterial liquid reaches 0.5 - 0.8 OD600; S03 Inoculate the activated bacterial liquid into the fermenter at a ratio of 5 - 10 v / v%; carry out aerobic fermentation at 37 - 42°C for 48 - 72 hours, after terminating the fermentation, cool the medium to 4°C to inhibit the metabolism of the bacteria; then centrifuge at 6000 rpm for 15 minutes to remove the bacteria, and collect the supernatant; use 0.45 μm microfiltration to further remove cell debris.

2. The instant whitening composition according to claim 1, wherein The mass ratio of the yeast / rice ferment filtrate, butylene glycol, p-hydroxyacetophenone and 1,2-hexanediol is: (40 - 60):(5 - 35):0.5:0.

5.

3. The instant whitening composition according to claim 1, wherein The mass ratio of the yeast / rice ferment filtrate, butylene glycol, p-hydroxyacetophenone and 1,2-hexanediol is: 49:15:0.5:0.

5.

4. The instant whitening composition according to any one of claims 1 to 3, characterized in that, The preparation method of the yeast / rice ferment filtrate is as follows: (1) Select brown rice, soak it in 3 times the volume of pure water for 12 hours, after high-pressure steaming for 30 minutes, add 0.5% w / v α-amylase and keep it at 65°C for 2 hours, and then cool it to below 30°C; (2) Yeast culture: In a medium containing nitrogen source, glucose and trace elements, add the cooled brown rice from step (1), and at the same time inoculate 10 6 CFU / mL of yeast, and perform aerobic fermentation at 28 °C for 48 hours; (3) Low-temperature extraction: After the fermentation is completed, centrifuge and collect the supernatant, and use low-temperature membrane filtration to obtain the filtrate; use the low-temperature vacuum concentration technology to obtain the yeast / rice ferment filtrate.

5. The instant whitening composition according to claim 4, characterized in that, The components of the medium in the step (2) are: wolfberry extract 5 - 10 g / L, glucose 5 - 15 g / L, yeast extract 5 - 10 g / L, peptone 3 - 8 g / L, ammonium sulfate 2 - 5 g / L, potassium dihydrogen phosphate 1 - 3 g / L, magnesium sulfate heptahydrate 0.5 - 1.5 g / L, L-cysteine 0.1 - 0.5 g / L, vitamin B1 0.01 - 0.5 g / L, ZnSO4 0.005 - 0.01 g / L, make up the volume to 1 L with water.

6. The instant whitening composition according to claim 1, wherein The preparation method of the rose damascena flower water is as follows: Select Rosa damascena petals, dry them by hot air at 40 - 50 °C, and crush them to 50 - 100 mesh; add ethanol - glycerol solvent in a ratio of 1:10 w / v; extract at 60 °C with a stirring speed of 100 rpm for 2 hours; then add 0.5% w / v pectinase and 0.3% w / v cellulase and extract at 50 °C for 1 hour; then filter using a 0.45 μm microfiltration membrane and concentrate at a temperature below 50 °C and - 0.08 MPa to 1 / 4 - 1 / 5 of the original volume.

7. The instant whitening composition according to any one of claims 1-6, wherein For the field of daily chemical products.

8. An instant whitening facial mask, characterized in that, Containing the instant whitening composition according to any one of claims 1 - 7.

9. The instant whitening facial mask according to claim 8, characterized in that, Its preparation raw materials include: Component A: deionized water, butanediol, glycerol, allantoin, carbomer 940, 1.3 million HA, p - hydroxyacetophenone, xanthan gum; Component B: PC 2000; Component C: 1,2 - hexanediol, compound plant anti - allergy agent, Portulaca oleracea extract, the instant whitening composition according to any one of claims 1 - 7.

Images