Preparation method of active water in lactoferrin purification

Abstract

The invention relates to the technical field of active water preparation, and discloses a preparation method of active water in lactoferrin purification, which comprises pretreatment, ultrafiltration treatment, reverse osmosis treatment, ion exchange treatment, mineralization treatment, magnetization treatment, ultraviolet sterilization treatment and ozone treatment. Large-particle impurities and organic pollutants in purified tail liquid are removed through cooperation of a sand filter and an activated carbon filter, water is deeply purified through ultrafiltration and reverse osmosis technologies, the ion content in water is reduced through ion exchange treatment, and mineral substances are adjusted through mineralization treatment to balance the pH value; water molecule clusters are reduced through magnetization treatment so as to enhance the dissolution mass transfer efficiency, and ultraviolet and ozone sterilization synergistically guarantee the water quality safety and improve the oxidation-reduction potential, so that the drinking standard is met.

Description

Technical Field

[0001] The present invention relates to the technical field of active water preparation, and specifically to a method for preparing active water in the purification of lactoferrin. Background Art

[0002] Lactoferrin is a glycoprotein with various biological activities and an important non-heme iron-binding glycoprotein in milk. Lactoferrin has powerful biological functions such as broad-spectrum antibacterial, antioxidant, anti-cancer, and immune system regulation, and has wide applications in the fields of food, medicine, etc. For example, in the food field, lactoferrin can be used as a food additive to enhance the nutritional value and functionality of food. In the medical field, lactoferrin can be used to prepare drugs with antibacterial, antioxidant, and other effects; With the continuous expansion of the application of lactoferrin, its demand is also increasing day by day. Currently, lactoferrin is mainly purified from cow's milk. However, a large amount of purification tail liquid generated after the purification of lactoferrin from cow's milk has not been properly treated and utilized. These purification tail liquids contain certain amounts of organic matter, suspended solids and other impurities. If directly discharged, it will not only cause eutrophication of water bodies due to high organic matter load, but also the deposition of suspended solids will damage the water ecosystem, not only polluting the environment but also wasting water resources. Therefore, it is urgent to develop an efficient treatment process for lactoferrin purification tail liquid; In view of this, in response to the above problems, in-depth research has led to the proposal of a method for preparing active water in the purification of lactoferrin. Summary of the Invention

[0003] The purpose of the present invention is to provide a method for preparing active water in the purification of lactoferrin to solve the problem that the purification tail liquid generated after the existing lactoferrin purification has not been properly treated and utilized as mentioned in the above background art.

[0004] To achieve the above purpose, the present invention provides the following technical solution: A method for preparing active water in the purification of lactoferrin, comprising the following steps: Step 1. Pretreatment: The purification tail liquid remaining after the purification of lactoferrin in cow's milk is first filtered through a sand filter to remove large particle impurities and suspended solids in the purification tail liquid, and then through an activated carbon filter to adsorb impurities such as organic matter, pigment, and odor in the purification tail liquid; Step 2. Ultrafiltration treatment: The pretreated purification is passed through an ultrafiltration membrane module to further remove colloids, bacteria, viruses, and macromolecular organic matter in the water, obtaining ultrafiltrated water; Step 3. Reverse osmosis treatment: The ultrafiltrated water is introduced into a reverse osmosis membrane device to remove small molecule impurities in the water, obtaining reverse osmosis water; Step 4. Ion exchange treatment: The reverse osmosis water is subjected to ion exchange treatment using cation exchange resin and anion exchange resin to remove cations and anions in the water; Step Five: Mineralization treatment: Add food-grade mineral additives to the water after ion exchange to make the water contain appropriate minerals beneficial to the human body and adjust the pH value of the water. Step Six: Magnetization treatment: Pass the mineralized water through a magnetization device to make the water molecular clusters smaller and enhance the activity of the water. Step Seven: Ultraviolet sterilization treatment: Conduct ultraviolet sterilization treatment on the magnetized water to kill bacteria and microorganisms in the water. Step Eight: Ozone treatment: Introduce ozone into the sterilized water for oxidation treatment to further remove organic substances and odors in the water, improve the activity of the water, and obtain active water.

[0005] Adopting the above technical solution, it is convenient to obtain active water that can improve the purification yield and purity of lactoferrin through multi-stage treatment of water.

[0006] As a preferred technical solution of the present invention, the sand filter material of the sand filter in Step One is quartz sand, with a particle size of 0.5 - 1.2 mm, a filtration speed of 5 - 6 m / h, the activated carbon of the activated carbon filter has a particle size of 1 - 2.5 mm, and a filtration speed of 3 - 4 m / h.

[0007] Adopting the above technical solution, it is convenient to efficiently remove large particle impurities and organic pollutants in the purification tail liquid and provide a clean water source for subsequent treatment.

[0008] As a preferred technical solution of the present invention, the cut-off molecular weight of the ultrafiltration membrane in Step Two is 2000 - 5000 Da, the membrane area of the ultrafiltration membrane is 10 - 15 m², the operating pressure is 0.1 - 0.15 MPa, and the temperature is 25 - 28 °C.

[0009] Adopting the above technical solution, it is convenient to efficiently separate colloids, bacteria, and macromolecular impurities in the water and ensure the permeability of subsequent lactoferrin.

[0010] As a preferred technical solution of the present invention, the membrane area of the reverse osmosis membrane in Step Three is 5 - 8 m², the operating pressure is 1.5 - 1.8 MPa, and the temperature is 25 - 28 °C.

[0011] Adopting the above technical solution, it is convenient to deeply remove inorganic salts and heavy metal ions in the water and ensure the water quality purity to meet the high-precision purification requirements.

[0012] As a preferred technical solution of the present invention, the volume ratio of the cation exchange resin to the anion exchange resin in Step Four is 1:1, and the flow rate of the exchange column is controlled at 3 - 5 L / min.

[0013] Adopting the above technical solution, it is convenient to efficiently remove residual ions in the water and improve the purity of the water.

[0014] As a preferred technical solution of the present invention, in step five, the mineral additive is calcium carbonate or magnesium chloride, the total content of minerals is 5-15 mg / L, and the pH value of water is 6.5-7.5.

[0015] Adopting the above technical solution facilitates the adjustment of the ionic balance and pH value of water quality to meet the structural stability requirements of lactoferrin.

[0016] As a preferred technical solution of the present invention, in step six, the magnetic field strength is 500-1000 Gs, and the flow rate of water in the magnetization device is 0.5-1.5 m / s.

[0017] Adopting the above technical solution facilitates the reduction of water molecular clusters through magnetization, significantly enhancing the dissolution and mass transfer efficiency of water.

[0018] As a preferred technical solution of the present invention, in step seven, the wavelength of ultraviolet light is 254 nm, the irradiation intensity is 30-40 mW / cm², and the irradiation time is 8-15 min.

[0019] Adopting the above technical solution facilitates the efficient killing of microorganisms in water while avoiding damage to lactoferrin caused by chemical residues.

[0020] As a preferred technical solution of the present invention, in step eight, the input amount of ozone is 1-3 mg / L, and the treatment time is 10-20 min.

[0021] Adopting the above technical solution facilitates the further purification of water quality and the improvement of water activity through the strong oxidizing property of ozone.

[0022] Compared with the prior art, the beneficial effects of the present invention are as follows: The preparation method of the lactoferrin purified active water systematically improves the purity and activity of water through a multi-stage treatment process. The sand filter and activated carbon filter cooperate to remove large particle impurities and organic pollutants in the purified tail liquid. Ultrafiltration and reverse osmosis technologies deeply purify the water quality. Ion exchange treatment reduces the ion content in water. Mineralization treatment adjusts the mineral balance and pH value. Magnetization treatment reduces water molecular clusters to enhance the dissolution and mass transfer efficiency. Ultraviolet and ozone sterilization cooperate to ensure water quality safety and improve the redox potential, meeting the drinkable standard. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 It is a schematic flow chart of the present invention. DETAILED DESCRIPTION OF THE INVENTION

[0024] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention. Embodiment 1

[0025] Please refer to Figure 1 , the technical solution of the present invention: A method for preparing active water in the purification of lactoferrin, comprising the following steps: Step 1. Pretreatment: The purified tail liquid remaining after the purification of lactoferrin in milk is first filtered through a sand filter to remove large particle impurities and suspended matters in the purified tail liquid, and then through an activated carbon filter to adsorb impurities such as organic matters, pigments, and peculiar smells in the purified tail liquid; Step 2. Ultrafiltration treatment: The pretreated purification is passed through an ultrafiltration membrane module to further remove colloids, bacteria, viruses, and macromolecular organic matters in the water, obtaining ultrafiltrated water; Step 3. Reverse osmosis treatment: The ultrafiltrated water is introduced into a reverse osmosis membrane device to remove small molecule impurities in the water, obtaining reverse osmosis water; Step 4. Ion exchange treatment: The reverse osmosis water is subjected to ion exchange treatment using cation exchange resin and anion exchange resin to remove cations and anions in the water; Step 5. Mineralization treatment: Food-grade mineral additives are added to the water after ion exchange to make the water contain an appropriate amount of minerals beneficial to the human body and adjust the pH value of the water; Step 6. Magnetization treatment: The mineralized water is passed through a magnetization device to make the water molecular clusters smaller and enhance the activity of the water; Step 7. Ultraviolet sterilization treatment: The magnetized water is subjected to ultraviolet sterilization treatment to kill bacteria and microorganisms in the water; Step 8. Ozone treatment: Ozone is introduced into the water after sterilization for oxidation treatment to further remove organic matters and peculiar smells in the water and improve the activity of the water, obtaining active water.

[0026] Wherein, the sand filter material of the sand filter in Step 1 is quartz sand with a particle size of 0.5 mm and a filtration speed of 5 m / h, and the activated carbon of the activated carbon filter has a particle size of 1 mm and a filtration speed of 3 m / h; Wherein, in Step 2, the cut-off molecular weight of the ultrafiltration membrane is 2000 Da, the membrane area of the ultrafiltration membrane is 10 m², the operating pressure is 0.1 MPa, and the temperature is 25 °C; Wherein, in Step 3, the membrane area of the reverse osmosis membrane is 5 m², the operating pressure is 1.5 MPa, and the temperature is 25 °C; In step four, the volume ratio of the cation exchange resin to the anion exchange resin is 1:1, and the flow rate of the exchange column is controlled at 3 L / min; In step five, the mineral additive is calcium carbonate or magnesium chloride, the total content of minerals is 5 mg / L, and the pH value of the water is 6.5; In step six, the magnetic field strength is 500 Gs, and the flow rate of water in the magnetization device is 0.5 m / s; In step seven, the wavelength of the ultraviolet light is 254 nm, the irradiation intensity is 30 mW / cm², and the irradiation time is 15 min; In step eight, the input amount of ozone is 1 mg / L, and the treatment time is 20 min. Example Two

[0027] Please refer to Figure 1 , the technical solution of the present invention: A method for preparing active water in the purification of lactoferrin, comprising the following steps: Step 1. Pretreatment: The purification tail liquid remaining after the purification of lactoferrin in milk is first filtered through a sand filter to remove large particle impurities and suspended matters in the purification tail liquid, and then through an activated carbon filter to adsorb impurities such as organic matters, pigments, and peculiar smells in the purification tail liquid; Step 2. Ultrafiltration treatment: The pretreated purification is passed through an ultrafiltration membrane module to further remove colloids, bacteria, viruses, and macromolecular organic matters in the water, obtaining ultrafiltration water; Step 3. Reverse osmosis treatment: The ultrafiltration water is introduced into a reverse osmosis membrane device to remove small molecule impurities in the water, obtaining reverse osmosis water; Step 4. Ion exchange treatment: Use cation exchange resin and anion exchange resin to perform ion exchange treatment on the reverse osmosis water to remove cations and anions in the water; Step 5. Mineralization treatment: Add a food-grade mineral additive to the water after ion exchange to make the water contain an appropriate amount of minerals beneficial to the human body and adjust the pH value of the water; Step 6. Magnetization treatment: Pass the mineralized water through a magnetization device to make the water molecular clusters smaller and enhance the activity of the water; Step 7. Ultraviolet sterilization treatment: Perform ultraviolet sterilization treatment on the magnetized water to kill bacteria and microorganisms in the water; Step 8. Ozone treatment: Introduce ozone into the sterilized water for oxidation treatment to further remove organic matters and peculiar smells in the water and improve the activity of the water, obtaining active water.

[0028] In step one, the sand filter material of the sand filter is quartz sand, the particle size is 0.8 mm, the filtration speed is 5 m / h, the particle size of the activated carbon in the activated carbon filter is 1.5 mm, and the filtration speed is 3 m / h; In Step 2, the molecular weight cut-off of the ultrafiltration membrane is 3000 Da, the membrane area of the ultrafiltration membrane is 12 m², the operating pressure is 0.12 MPa, and the temperature is 26 °C; In Step 3, the membrane area of the reverse osmosis membrane is 5 m², the operating pressure is 1.6 MPa, and the temperature is 26 °C; In Step 4, the volume ratio of the cation exchange resin to the anion exchange resin is 1:1, and the flow rate of the exchange column is controlled at 3 L / min; In Step 5, the mineral additive is calcium carbonate or magnesium chloride, the total content of minerals is 8 mg / L, and the pH value of the water is 6.8; In Step 6, the magnetic field strength is 600 Gs, and the flow rate of water in the magnetization device is 0.8 m / s; In Step 7, the wavelength of the ultraviolet light is 254 nm, the irradiation intensity is 35 mW / cm², and the irradiation time is 12 min; In Step 8, the input amount of ozone is 2 mg / L, and the treatment time is 16 min. Example 3

[0029] Please refer to Figure 1 , the technical solution of the present invention: A method for preparing active water in the purification of lactoferrin, comprising the following steps: Step 1, Pretreatment: The purification tail liquid remaining after the purification of lactoferrin in milk is first filtered through a sand filter to remove large particle impurities and suspended matters in the purification tail liquid, and then through an activated carbon filter to adsorb impurities such as organic matters, pigments, and odors in the purification tail liquid; Step 2, Ultrafiltration treatment: The pretreated purification is passed through an ultrafiltration membrane module to further remove colloids, bacteria, viruses, and macromolecular organic matters in the water, obtaining ultrafiltration water; Step 3, Reverse osmosis treatment: The ultrafiltration water is introduced into a reverse osmosis membrane device to remove small molecule impurities in the water, obtaining reverse osmosis water; Step 4, Ion exchange treatment: Use cation exchange resin and anion exchange resin to perform ion exchange treatment on the reverse osmosis water to remove cations and anions in the water; Step 5, Mineralization treatment: Add a food-grade mineral additive to the water after ion exchange to make the water contain an appropriate amount of minerals beneficial to the human body and adjust the pH value of the water; Step 6, Magnetization treatment: Pass the mineralized water through a magnetization device to make the water molecular clusters smaller and enhance the activity of the water; Step 7, Ultraviolet sterilization treatment: Perform ultraviolet sterilization treatment on the magnetized water to kill bacteria and microorganisms in the water; Step 8, Ozone treatment: Pass ozone into the sterilized water for oxidation treatment to further remove organic matters and odors in the water and improve the activity of the water, obtaining active water.

[0030] In step one, the sand filter medium of the sand filter is quartz sand with a particle size of 1.0 mm and a filtration rate of 6 m / h. The activated carbon of the activated carbon filter has a particle size of 2.2 mm and a filtration rate of 4 m / h; In step two, the ultrafiltration membrane has a cut-off molecular weight of 4000 Da, a membrane area of 12 m², an operating pressure of 0.12 MPa, and a temperature of 28 °C; In step three, the reverse osmosis membrane has a membrane area of 7 m², an operating pressure of 1.7 MPa, and a temperature of 27 °C; In step four, the volume ratio of cation exchange resin to anion exchange resin is 1:1, and the flow rate of the exchange column is controlled at 5 L / min; In step five, the mineral additive is calcium carbonate or magnesium chloride, the total mineral content is 12 mg / L, and the pH value of the water is 7.2; In step six, the magnetic field strength is 800 Gs, and the flow rate of water in the magnetization device is 1.2 m / s; In step seven, the wavelength of the ultraviolet light is 254 nm, the irradiation intensity is 35 mW / cm², and the irradiation time is 10 min; In step eight, the ozone input amount is 3 mg / L, and the treatment time is 12 min. Example Four

[0031] Please refer to Figure 1 , the technical solution of the present invention: A method for preparing active water in the purification of lactoferrin, comprising the following steps: Step one, pretreatment: The purification tail liquid remaining after the purification of lactoferrin in milk is first filtered through a sand filter to remove large particle impurities and suspended matters in the purification tail liquid, and then through an activated carbon filter to adsorb impurities such as organic matters, pigments, and odors in the purification tail liquid; Step two, ultrafiltration treatment: The pretreated purification is passed through an ultrafiltration membrane module to further remove colloids, bacteria, viruses, and macromolecular organic matters in the water, obtaining ultrafiltration water; Step three, reverse osmosis treatment: The ultrafiltration water is introduced into a reverse osmosis membrane device to remove small molecule impurities in the water, obtaining reverse osmosis water; Step four, ion exchange treatment: Cation exchange resin and anion exchange resin are used to perform ion exchange treatment on the reverse osmosis water to remove cations and anions in the water; Step five, mineralization treatment: A food-grade mineral additive is added to the water after ion exchange to make the water contain an appropriate amount of minerals beneficial to the human body and adjust the pH value of the water; Step six, magnetization treatment: The mineralized water is passed through a magnetization device to make the water molecular clusters smaller and enhance the activity of the water; Step 7. Ultraviolet sterilization treatment: Perform ultraviolet sterilization treatment on the magnetized water to kill bacteria and microorganisms in the water; Step 8. Ozone treatment: Introduce ozone into the sterilized water for oxidation treatment to further remove organic substances and odors in the water, improve the activity of the water, and obtain active water.

[0032] Among them, the sand filter material of the sand filter in Step 1 is quartz sand, with a particle size of 1.2 mm and a filtration speed of 6 m / h. The activated carbon of the activated carbon filter has a particle size of 2.5 mm and a filtration speed of 4 m / h; Among them, the cut-off molecular weight of the ultrafiltration membrane in Step 2 is 5000 Da, the membrane area of the ultrafiltration membrane is 15 m², the operating pressure is 0.15 MPa, and the temperature is 28 °C; Among them, the membrane area of the reverse osmosis membrane in Step 3 is 8 m², the operating pressure is 1.8 MPa, and the temperature is 28 °C; Among them, the volume ratio of the cation exchange resin to the anion exchange resin in Step 4 is 1:1, and the flow rate of the exchange column is controlled at 5 L / min; Among them, the mineral additive in Step 5 is calcium carbonate or magnesium chloride, the total content of minerals is 15 mg / L, and the pH value of the water is 7.5; Among them, the magnetic field strength in Step 6 is 1000 Gs, and the flow rate of water in the magnetization device is 1.5 m / s; Among them, the wavelength of ultraviolet light in Step 7 is 254 nm, the irradiation intensity is 40 mW / cm², and the irradiation time is 8 min; Among them, the ozone input amount in Step 8 is 3 mg / L, and the treatment time is 10 min.

[0033] Active water detection The active water prepared in the above examples will be tested according to the "Hygienic Standard for Drinking Water" GB 5749-2022, and the results are shown in the following table:

[0034] It can be seen from the test results that the prepared active water is tested, and all its indicators meet the national hygienic standard for drinking water, proving that the active water prepared by the present invention meets the drinkable standard; the conductivity reflects the water quality purity, and the active water prepared in the examples ≤ 8.5 μS / cm, indicating that reverse osmosis and ion exchange treatment effectively remove inorganic salts; the calcium and magnesium ion concentrations meet the food-grade standard and can adapt to the stable environment of lactoferrin; the total number of bacteria is < 10 CFU / mL, proving the synergistic effect of ultraviolet and ozone sterilization; the oxidation-reduction potential is negative (-85 to -130 mV), reflecting the antioxidant property of active water and inhibiting the oxidation of lactoferrin; the water molecular cluster size is 55 - 75 nm, indicating that the magnetization treatment effectively reduces the water molecular cluster and enhances the dissolution and mass transfer efficiency of water.

[0035] The content not described in detail in this specification belongs to the prior art well-known to those skilled in the art.

[0036] Although the embodiments of the present invention have been shown and described, those of ordinary skill in the art can understand that various changes, modifications, substitutions, and variations can be made to these embodiments without departing from the principles and spirit of the present invention. The scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A method for preparing active water in the purification of lactoferrin, characterized in that, It includes the following steps: Step 1, Pretreatment: The purified tail liquid remaining after the purification of lactoferrin in milk is first filtered through a sand filter to remove large particle impurities and suspended matters in the purified tail liquid, and then through an activated carbon filter to adsorb impurities such as organic matters, pigments, and peculiar smells in the purified tail liquid; Step 2, Ultrafiltration treatment: The purified liquid after pretreatment is passed through an ultrafiltration membrane module to further remove colloids, bacteria, viruses, and macromolecular organic matters in the water, obtaining ultrafiltered water; Step 3, Reverse osmosis treatment: The ultrafiltered water is introduced into a reverse osmosis membrane device to remove small molecule impurities in the water, obtaining reverse osmosis water; Step 4, Ion exchange treatment: The reverse osmosis water is subjected to ion exchange treatment using cation exchange resin and anion exchange resin to remove cations and anions in the water; Step 5, Mineralization treatment: Food-grade mineral additives are added to the water after ion exchange to make the water contain appropriate minerals beneficial to the human body and adjust the pH value of the water; Step 6, Magnetization treatment: The mineralized water is passed through a magnetization device to make the water molecular clusters smaller and enhance the activity of the water; Step 7, Ultraviolet sterilization treatment: The magnetized water is subjected to ultraviolet sterilization treatment to kill bacteria and microorganisms in the water; Step 8, Ozone treatment: Ozone is introduced into the sterilized water for oxidation treatment to further remove organic matters and peculiar smells in the water and improve the activity of the water, obtaining active water.

2. The preparation method of active water in the purification of lactoferrin according to claim 1, characterized in that, In Step 1, the sand filter material of the sand filter is quartz sand with a particle size of 0.5 - 1.2 mm and a filtration speed of 5 - 6 m / h, and the activated carbon particle size of the activated carbon filter is 1 - 2.5 mm and the filtration speed is 3 - 4 m / h.

3. The preparation method of active water in the purification of lactoferrin according to claim 1, characterized in that, In Step 2, the cut-off molecular weight of the ultrafiltration membrane is 2000 - 5000 Da, the membrane area of the ultrafiltration membrane is 10 - 15 m², the operating pressure is 0.1 - 0.15 MPa, and the temperature is 25 - 28 °C.

4. The preparation method of active water in the purification of lactoferrin according to claim 1, characterized in that, In Step 3, the membrane area of the reverse osmosis membrane is 5 - 8 m², the operating pressure is 1.5 - 1.8 MPa, and the temperature is 25 - 28 °C.

5. The preparation method of active water in the purification of lactoferrin according to claim 1, characterized in that, In Step 4, the volume ratio of the cation exchange resin to the anion exchange resin is 1:1, and the flow rate of the exchange column is controlled at 3 - 5 L / min.

6. The preparation method of active water in the purification of lactoferrin according to claim 1, characterized in that, In Step 5, the mineral additive is calcium carbonate or magnesium chloride, the total content of minerals is 5 - 15 mg / L, and the pH value of the water is 6.5 - 7.

5.

7. The preparation method of active water in the purification of lactoferrin according to claim 1, characterized in that, In Step 6, the magnetic field intensity is 500 - 1000 Gs, and the flow rate of the water in the magnetization device is 0.5 - 1.5 m / s.

8. A method for preparing active water in the purification of lactoferrin according to claim 1, characterized in that, In Step 7, the wavelength of the ultraviolet ray is 254 nm, the irradiation intensity is 30 - 40 mW / cm², and the irradiation time is 8 - 15 min.

9. The preparation method of active water in the purification of lactoferrin according to claim 1, characterized in that, In Step 8, the ozone input amount is 1 - 3 mg / L, and the treatment time is 10 - 20 min.

Images