Microorganism culture solution, ash layer curing liquid and post-fire debris flow prevention and control method

Abstract

The invention discloses a microbial culture solution, an ash layer curing solution and a post-fire debris flow prevention and control method, and relates to the technical field of debris flow prevention and control. The microbial culture solution comprises the following components in parts by weight: 10-20 parts of peptone; 5 to 10 parts of glucose; 5-7 parts of sodium chloride; 1000 parts of deionized water; the regulating agent is used for regulating the acid-base property of culture; 1-2 parts of a buffer agent; wherein the pH value of the culture solution is 8-10, and the culture solution can provide a nutrient source for the ash layer solidified microorganisms and ensure that the ash layer solidified microorganisms have activity and continuously proliferate, so that the ash layer solidified microorganisms can effectively solidify the ash layer after forest fire. The ash layer curing liquid comprises the following components in parts by weight: 1000 parts of the microbial culture solution; 15 to 25 parts of immobilized microorganisms, wherein the immobilized microorganisms are bacillus subtilis and / or bacillus coriolis; and 30-50 parts of a calcium source. According to the post-fire debris flow prevention and treatment method, the ash layer curing liquid is sprayed on a post-fire slope.

Description

Technical Field

[0001] The present invention relates to the technical field of debris flow prevention and control, and particularly relates to a microbial culture solution, an ash layer solidification solution, and a method for preventing and controlling post-fire debris flow. Background Art

[0002] Post-fire debris flow refers to debris flow related to forest fires occurring in burned areas. Compared with other debris flows, due to the high temperature generated by the combustion of soil vegetation during forest fires, various properties of the soil change, and the soil becomes soft. The burned vegetation also brings a large amount of ash layer, which provides a rich material source for the generation of post-fire debris flow. At the same time, forest fires often occur 1 - 2 months before the rainy season, which poses a huge challenge to the treatment of the ash layer.

[0003] Existing technologies usually reduce the formation conditions of debris flow and the bulk density by cleaning the ash layer. Although the material source during the occurrence of post-fire debris flow is reduced, cleaning the entire burned area is a time-consuming and laborious project. However, the surface ash layer is rich in nutrients. If the ash layer can be fixed, changing it from a loose state to a consolidated state, it can not only reduce the material source amount of post-fire debris flow, reduce the probability and harmfulness of post-fire debris flow occurrence, but also provide nutrients for the scattered plant seeds, and can accelerate their growth trend to a certain extent.

[0004] It is known that the microbial-induced calcium carbonate precipitation technology is a technology that uses microorganisms to induce the conversion of ions such as calcium ions and magnesium ions in the environment into precipitates. This technology is often used for soil treatment and has good effects. Therefore, a technical solution for solidifying the ash layer after a forest fire by using microbial-induced calcium carbonate precipitation can be studied. Summary of the Invention

[0005] The present invention provides a microbial culture solution, an ash layer solidification solution, and a method for preventing and controlling post-fire debris flow, which can use the microbial-induced precipitation technology to fix loose accumulations, reduce the risk of becoming the starting material source of post-fire debris flow, improve the treatment effect on post-fire debris flow, and has the characteristics of environmental friendliness and low cost.

[0006] The present invention is achieved by the following technical solutions:

[0007] In a first aspect, the present invention provides a microbial culture solution, comprising the following components in parts by weight:

[0008] Peptone 10 - 20 parts;

[0009] Glucose 5 - 10 parts;

[0010] Sodium chloride 5 - 7 parts;

[0011] Adjusting agent 2 - 5 parts, used to adjust the acidity and alkalinity of the culture;

[0012] 1 - 2 parts of buffer agent;

[0013] Among them, the pH value of the culture solution is 8 - 10.

[0014] It should be noted that the ash layer solidifying microorganism has the ability to induce mineral precipitation. Moreover, since the soil after the fire is alkaline, microorganisms that can resist alkalinity are needed so that they can maintain activity and have a certain proliferation ability in a highly alkaline environment; in addition, the ash layer solidifying microorganism should also have the mineralization ability to induce calcium carbonate precipitation, which can consolidate loose soil and prevent it from becoming the source of debris flow after the fire.

[0015] In view of this, the microbial culture solution provided by the present invention includes peptone, glucose, sodium chloride, deionized water, regulating agent and buffer agent, and the pH value of the culture solution is 8 - 10. Among them, peptone can provide nitrogen source and amino acids for the ash layer solidifying microorganism, and support the synthesis of proteins, nucleic acids and other nitrogen-containing compounds; glucose can provide carbon source and energy for the ash layer solidifying microorganism, and support cell metabolism and growth; sodium chloride can provide sodium ions and chloride ions for the ash layer solidifying microorganism to maintain the osmotic pressure balance of cells; the regulator is used to adjust the pH of the culture solution to alkaline; the buffer agent is used to maintain the pH stability of the culture medium and can prevent the pH from fluctuating during mixed culture.

[0016] Therefore, the microbial culture solution provided by the present invention can provide a nutrient source for the ash layer solidifying microorganism, ensure its activity and continuous proliferation, so as to ensure that the ash layer solidifying microorganism can effectively solidify the ash layer after the wildfire, facilitate the use of the microbial-induced precipitation technology to fix the loose accumulation body, reduce the risk of it becoming the starting source of debris flow after the fire, and improve the treatment effect on debris flow after the fire.

[0017] In an alternative embodiment, the pH value of the culture solution is 8.5 - 9.

[0018] In an alternative embodiment, the regulating agent is sodium carbonate, which is convenient for gently adjusting the alkalinity of the culture solution.

[0019] In an alternative embodiment, the buffer agent is potassium dihydrogen phosphate.

[0020] In an alternative embodiment, the weight part of peptone is 10 parts, the weight part of glucose is 5 parts, the weight part of sodium chloride is 5 parts, the weight part of the buffer agent is 1 part, and it also includes 1000 parts of deionized water by weight.

[0021] In the second aspect, the present invention provides an ash layer solidifying liquid, which includes the following components in parts by weight:

[0022] 1000 copies of the above-mentioned microbial culture solution;

[0023] 15-25 parts of solidified microorganisms, wherein the solidified microorganisms are Bacillus subtilis and / or Bacillus cohnii;

[0024] 30-50 portions of calcium source.

[0025] It should be noted that microorganisms such as Bacillus pasteurianus, Sporosarcina pasteurianus, Bacillus subtilis, and Bacillus cohnii are not only widely present in the natural environment, but also have good tolerance to alkaline environments. Among them, Bacillus pasteurianus (suitable pH = 7.5-9.5) and Sporosarcina pasteurianus (suitable pH = 7.5-9.5) are suitable for weakly alkaline environments, and Bacillus subtilis (suitable pH = 6-9) and Bacillus cohnii (suitable pH = 7-10) can survive in highly alkaline environments. However, since the cultivation cost of Bacillus pasteurianus and Sporosarcina pasteurianus is too high compared to the latter, they are not suitable for large-scale production.

[0026] In this regard, the present invention uses Bacillus subtilis and Bacillus cohnii as ash layer solidifying microorganisms, and cultivates them through the above-mentioned microbial culture solution, and combines them with a calcium source to form an ash layer solidifying liquid, and the weight parts of the above-mentioned microbial culture solution are 1000 parts, the weight parts of the solidifying microorganisms are 15-25 parts, and the weight parts of the calcium source are 30-50 parts. The solidification application of the ash layer solidifying microorganisms can be achieved, thereby fixing the loose deposits and reducing the risk of them becoming the starting source of debris flow after the fire.

[0027] In an optional embodiment, the calcium source is one of calcium chloride, calcium nitrate and calcium lactate.

[0028] In an optional embodiment, the weight portion of the immobilized microorganisms is 20 parts, and the weight portion of the calcium source is 40 parts.

[0029] In an optional embodiment, the immobilized microorganisms include Bacillus subtilis and Bacillus cohnii, wherein the ratio of Bacillus subtilis to Bacillus cohnii is 1: 1. The present invention mixes Bacillus subtilis and Bacillus cohnii, which can improve their tolerance range to alkaline environment (pH = 6 to 10), and has better activity and mineralization ability than a single strain.

[0030] In a third aspect, the present invention provides a method for preventing and controlling post-fire debris flows, in which the above-mentioned ash layer solidification liquid is sprayed on the slope surface after the fire, so as to fix the loose accumulation body through the precipitation induced by the ash layer solidification microorganisms, thereby reducing the risk of it becoming a starting source of post-fire debris flows and improving the control effect of post-fire debris flows.

[0031] Compared with the prior art, the present invention has the following advantages and beneficial effects:

[0032] 1. The microbial culture medium provided by the present invention comprises peptone, glucose, sodium chloride, deionized water, a regulating agent and a buffering agent, and the pH value of the culture medium is 8 - 10. Among them, peptone can provide nitrogen source and amino acids for the microorganisms solidifying the ash layer, supporting the synthesis of proteins, nucleic acids and other nitrogen-containing compounds; glucose can provide carbon source and energy for the microorganisms solidifying the ash layer, supporting cell metabolism and growth; sodium chloride can provide sodium ions and chloride ions for the microorganisms solidifying the ash layer, maintaining the osmotic pressure balance of cells; the regulator is used to adjust the pH of the culture medium to alkaline; the buffering agent is used to maintain the pH stability of the culture medium, can prevent the pH fluctuation in mixed culture, can provide nutrient sources for the microorganisms solidifying the ash layer, ensure their activity and continuous proliferation, so as to ensure that the microorganisms solidifying the ash layer can effectively solidify the ash layer after wildfire, facilitate the use of the microbial-induced precipitation technology to fix the loose accumulation body, reduce the risk of it becoming the starting source of post-fire debris flow, and improve the treatment effect on post-fire debris flow.

[0033] 2. The ash layer solidifying liquid provided by the present invention uses Bacillus subtilis and Bacillus kochii as the microorganisms for solidifying the ash layer, and is cultured with the above-mentioned microbial culture medium and in combination with a calcium source to form the ash layer solidifying liquid. And the weight parts of the above-mentioned microbial culture medium are 1000 parts, the weight parts of the solidifying microorganisms are 15 - 25 parts, and the weight parts of the calcium source are 30 - 50 parts, which can realize the solidifying application of the microorganisms for solidifying the ash layer, thereby playing a role in fixing the loose accumulation body and reducing the risk of it becoming the starting source of post-fire debris flow.

[0034] 3. The method for preventing and controlling post-fire debris flow provided by the present invention sprays the above-mentioned ash layer solidifying liquid on the slope after wildfire to play a role in fixing the loose accumulation body through microbial-induced precipitation of the microorganisms for solidifying the ash layer, reduce the risk of it becoming the starting source of post-fire debris flow, and improve the treatment effect on post-fire debris flow. Specific embodiments

[0035] To make the objectives, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are some, but not all, of the embodiments of the present invention. Therefore, the detailed description of the embodiments of the present invention is not intended to limit the scope of the claimed invention, but merely represents the selected embodiments of the present invention.

[0036] Embodiment 1

[0037] This embodiment provides a microbial culture medium, which comprises the following components in parts by weight:

[0038] 10 - 20 parts of peptone;

[0039] 5 - 10 parts of glucose;

[0040] 5 - 7 parts of sodium chloride;

[0041] 1000 parts of deionized water;

[0042] 2 - 5 parts of regulating agent, used to regulate the acidity and alkalinity of the culture;

[0043] 1 - 2 parts of buffer agent;

[0044] Among them, the pH value of the culture solution is 8 - 10.

[0045] It can be understood that the ash layer - solidified microorganisms have the ability to induce mineral precipitation. And since the soil after being burned by fire is alkaline, microorganisms that can resist alkalinity are needed so that they can maintain activity and have a certain proliferation ability in a highly alkaline environment; in addition, the ash layer - solidified microorganisms should also have the mineralization ability to induce calcium carbonate precipitation, which can consolidate loose soil and prevent it from becoming the source material of post - fire debris flows.

[0046] At the same time, microorganisms such as Bacillus pasteurii, Sarcina pasteurii, Bacillus subtilis, and Bacillus cohnii not only widely exist in the natural environment but also have good tolerance to alkaline environments. Among them, Bacillus pasteurii (suitable pH = 7.5 - 9.5) and Sarcina pasteurii (suitable pH = 7.5 - 9.5) are suitable for a slightly alkaline - preferred environment, and Bacillus subtilis (suitable pH = 6 - 9) and Bacillus cohnii (suitable pH = 7 - 10) can survive in a highly alkaline environment. However, due to the fact that the culture costs of Bacillus pasteurii and Sarcina pasteurii are too high compared to the latter, they are not suitable for large - scale production. Therefore, in this embodiment, Bacillus subtilis and Bacillus cohnii are used as ash layer - solidified microorganisms for cultivation, so that the pH value of the culture solution is 8.5 - 9.

[0047] In this embodiment, the regulating agent is sodium carbonate, which is convenient for gently regulating the alkalinity of the culture solution. The buffer agent is potassium dihydrogen phosphate. The weight part of peptone is 10 parts, the weight part of glucose is 5 parts, the weight part of sodium chloride is 5 parts, and the weight part of the buffer agent is 1 part. That is, the formula of the microbial culture solution provided in this embodiment is shown in Table 1.

[0048] Peptone / g Glucose / g Sodium chloride / g Deionized water / L Sodium carbonate / g Potassium dihydrogen phosphate / g pH 10 5 5 1 2～5 1 8.5～9

[0049] Table 1

[0050] In summary, the microbial culture medium provided in this embodiment includes peptone, glucose, sodium chloride, deionized water, a regulating agent, and a buffering agent, and the pH value of the culture medium is 8-10. Among them, peptone can provide nitrogen sources and amino acids for the microorganisms in the ash layer solidification, supporting the synthesis of proteins, nucleic acids, and other nitrogen-containing compounds; glucose can provide carbon sources and energy for the microorganisms in the ash layer solidification, supporting cell metabolism and growth; sodium chloride can provide sodium ions and chloride ions for the microorganisms in the ash layer solidification, maintaining the osmotic pressure balance of cells; the regulator is used to adjust the pH of the culture medium to alkaline; the buffering agent is used to maintain the pH stability of the culture medium, and can prevent pH fluctuations in the mixed culture.

[0051] Therefore, the microbial culture medium provided in this embodiment can provide a nutrient source for the microorganisms in the ash layer solidification, ensure their activity and continuous proliferation, so as to ensure that the microorganisms in the ash layer solidification can effectively solidify the ash layer after the wildfire, facilitate the use of the microbial-induced precipitation technology to fix the loose accumulation body, reduce the risk of it becoming the starting source of post-fire debris flow, and improve the treatment effect of post-fire debris flow.

[0052] Example 2

[0053] This embodiment provides an ash layer solidification liquid, which includes the following components in parts by weight:

[0054] 1000 parts of the microbial culture medium described in Example 1;

[0055] 15-25 parts of solidifying microorganisms, and the solidifying microorganisms are Bacillus subtilis and / or Bacillus cohnii;

[0056] 30-50 parts of calcium source.

[0057] It should be noted that microorganisms such as Bacillus pasteurii, Sarcina pasteurii, Bacillus subtilis, and Bacillus cohnii not only widely exist in the natural environment, but also have good tolerance to alkaline environments. Among them, Bacillus pasteurii (suitable pH = 7.5-9.5) and Sarcina pasteurii (suitable pH = 7.5-9.5) are suitable for weakly alkaline environments, and Bacillus subtilis (suitable pH = 6-9) and Bacillus cohnii (suitable pH = 7-10) can survive in highly alkaline environments. However, due to the too high culture cost of Bacillus pasteurii and Sarcina pasteurii compared with the latter, they are not suitable for large-scale production.

[0058] It is known that the calcium source for microbial-induced calcium carbonate precipitation provides the source of minerals for initial nucleation and crystallization, which can be calcium chloride, calcium nitrate, calcium lactate, magnesium chloride, etc. Therefore, it is understood that calcium nitrate has the disadvantages of high cost and environmental pollution, calcium lactate has the disadvantages of high cost and low precipitation efficiency, and magnesium chloride has the disadvantages of low precipitation efficiency and limited applicability. Compared with other calcium sources, calcium chloride has the advantages of high solubility, high efficiency, low cost, and mature technology, and is suitable for large-scale applications.

[0059] At the same time, the solidifying microorganisms include Bacillus subtilis and Bacillus cohnii, and among them, the ratio of Bacillus subtilis to Bacillus cohnii is 1:1. In the present invention, Bacillus subtilis and Bacillus cohnii are mixed, which can improve their tolerance range to alkaline environments (pH = 6 - 10), and have better activity and mineralization ability than single strains.

[0060] In addition, since microorganisms need to be activated and inoculated for cultivation before being used in engineering applications from freeze-dried powder to large-scale cultivation. When activating and culturing, agar needs to be added to the nutrient solution described in Example 1, and it is prepared into a slant medium for cultivation for 24 h. Inoculation and cultivation are carried out in a liquid medium. After 24 h of inoculation and cultivation, subculture can be carried out. The temperature for culturing microorganisms should be 28 ± 1 °C, and inoculation and cultivation need to be carried out in an oscillating incubator, and the rotation speed of the incubator should be 200 r / min; when activating and inoculating for cultivation, part of the microorganisms need to be reserved for cryopreservation for subsequent use. Before being put into use, a fermenter is needed, and the microorganisms are inoculated into a 1 m 3 tank for subculture until OD 600 ≈ 2.0, and then placed in a 10 m 3 tank for subculture until the bacterial liquid concentration reaches 10 9 CFU / mL, then it can be put into use.

[0061] When preparing the ash layer solidifying liquid, the ratio between the ash layer solidifying microorganisms and the nutrient solution described in Example 1 should be determined first, and based on adding the ash layer solidifying microorganisms to the nutrient solution described in Example 1, the OD value after 24 h is measured to obtain the best ratio relationship. The specific test scheme and results are shown in Table 2.

[0062] Number Microorganisms / mL Nutrient solution / kg OD value after 24 - hour culture 1 5 1 1.54 2 10 1 1.68 3 15 1 1.76 4 20 1 1.83 5 25 1 1.82

[0063] Table 2

[0064] As can be seen from Table 2, the OD value of the solidified microorganisms in the ash layer after 24 hours of cultivation shows a trend of first increasing and then decreasing with the increase of its content (dosage), reaching the maximum when the dosage is 20 mL, and the OD value after culturing for 24 hours with a dosage of 25 mL is 1.82. This indicates that due to the excessive addition of microorganisms in the early stage, rapid proliferation of microorganisms occurs. After 24 hours, the proliferation of microorganisms has tended to be stable and will not increase. Considering the cost issue, in this embodiment, the ratio of microorganisms to nutrient solution is 20 mL: 1 t.

[0065] Meanwhile, using loess as the test soil body, different ratios of solidifying liquid were sprayed on it respectively to conduct the microbial-induced calcium carbonate film-forming test. The average thickness of the film after 7 days was used to determine its precipitation effect. The specific test data and results are shown in Table 3.

[0066] Number Microorganisms / mL Nutrient solution / kg Calcium source / g Average thickness of the film / cm 1 20 1 10 1 2 20 1 20 1.6 3 20 1 30 1.9 4 20 1 40 2.2 5 20 1 50 2

[0067] Table 3

[0068] Combined with Table 2, it can be seen that the average film thickness shows a trend of first increasing and then decreasing with the increase of the calcium source. The film thickness is the best when the calcium source is added up to 40 g, and the average thickness is 2.2 cm. Therefore, according to the experimental results, the formula of the microbial solidifying liquid is selected as 20 mL of microorganisms, 1 kg of nutrient solution, and 40 g of calcium source. Considering the large dosage in engineering practice, the formula of the solidifying liquid after unit expansion is shown in Table 4, and it is formulated according to each ton of nutrient solution.

[0069] Component Dosage Remarks Microorganisms 20L Bacillus subtilis + Bacillus kochii (mixed at a ratio of 1:1) Nutrient solution 1t Provide nutrition for microorganisms Calcium source 30 - 50 kg Determine the dosage according to the over - fire situation and the amount of microorganisms added

[0070] Table 4

[0071] That is, in this embodiment, the weight portion of the solidified microorganisms is 20 portions, and the weight portion of the calcium source is 40 portions.

[0072] In summary, in this embodiment, Bacillus subtilis and Bacillus cohnii are used as the solidified microorganisms in the ash layer, cultured through the microbial culture solution recorded in Example 1, and combined with a calcium source to form an ash layer solidifying liquid. And the above-mentioned microbial culture solution has a weight portion of 1000 portions, the weight portion of the solidified microorganisms is 15 - 25 portions, and the weight portion of the calcium source is 30 - 50 portions, which can realize the solidification application of the solidified microorganisms in the ash layer, thereby playing a fixing role on the loose accumulation body and reducing the risk of it becoming the starting source of post-fire debris flow.

[0073] Example 3

[0074] This embodiment provides a method for preventing and controlling post-fire debris flow. Spraying the ash layer solidifying liquid recorded in Example 2 on the post-fire slope surface to play a fixing role on the loose accumulation body through microbial-induced precipitation in the ash layer, reduce the risk of it becoming the starting source of post-fire debris flow, and improve the treatment effect on post-fire debris flow.

[0075] Specifically, before spraying the ash layer solidifying liquid described in Embodiment 2, it is necessary to conduct a on-site survey of the area of the slope's burned soil, and based on the survey results, determine the quantity of the solidifying liquid, the layout position of the conveying device, the selection of the pipeline, and the pipeline layout.

[0076] Generally speaking, the conveying device for the ash layer solidifying liquid consists of a liquid storage tank and a conveying pump. The liquid storage tank is used to store the solidifying liquid, and corrosion-resistant stainless steel is used as the tank material. The volume of the tank is determined according to different burned areas; an air-operated stirrer is installed inside the liquid storage tank to prevent microbial sedimentation or component stratification. The conveying pump provides power to convey the solidifying liquid to the pipeline system. The conveying pump uses a centrifugal pump with parameter requirements. The liquid storage tank and the conveying pump are connected by pipelines.

[0077] Correspondingly, the pipeline system for the ash layer solidifying liquid consists of a main conveying pipeline and branch conveying pipelines. The main pipeline is usually made of high-density polyethylene material and has the characteristic of corrosion resistance. The branch pipelines are used for slopes with large burned areas, and fixing rings are set at certain intervals (usually at intervals of 1 m) to fix their positions. For slopes with small burned areas, the pipeline system can be replaced with PE hoses and high-pressure nozzles. The high-pressure nozzle is an atomizing nozzle, and the whole nozzle is made of stainless steel and has the characteristic of corrosion resistance. It consists of a nozzle, a control grip, and an interface. The nozzle generates a mist spray to facilitate the large-area diffusion of the solidifying liquid, and the control grip is used to adjust the spraying speed of the solidifying liquid.

[0078] Therefore, it is understood that for slopes with large burned areas after the fire, the method of laying pipelines is adopted: select a suitable location to place the conveying device, and reasonably lay the pipeline system composed of the main pipeline and branch pipelines on the slope. The principle of pipeline laying is that pipelines are laid densely at the slopes with serious fire conditions and many loose accumulations, and sparsely at the places with less serious fire conditions and fewer loose accumulations. The microbial solidifying liquid is in the liquid storage tank, and the conveying pump is used to convey the solidifying liquid to the laid pipeline system, so as to realize the spraying of the solidifying liquid on the loose accumulations on the slope.

[0079] For slopes with small burned areas, the method of manual spraying can be adopted: use the conveying device to convey the microbial solidifying liquid through the PE hose, install a high-pressure nozzle at the end of the PE hose, and use the manual holding method to spray the solidifying liquid. The spraying principle is to spray from top to bottom, spray multiple times, spray more at the places with more loose bodies and less at the places with less loose bodies.

[0080] Thus, the ash layer solidifying liquid configured in Embodiment 2 is sprayed on the loose accumulations by using the conveying device and the conveying pipeline, and the process of microbial-induced calcium carbonate precipitation is used to convert calcium ions, magnesium ions, etc. in the environment into carbonate precipitates, and their good cementing property is used to fix the loose accumulations.

[0081] In summary, in this embodiment, the above-mentioned ash layer solidification liquid is sprayed on the slope surface after the fire. The ash layer solidification microbial microenvironment in the ash layer solidification liquid has good tolerance and can act in the soil for a long time to fix the loose accumulation body through the induced precipitation of the ash layer solidification microorganisms.

[0082] It can be known that the induced precipitation minerals have better cementation than those generated by chemical methods and can better improve the fixation of the ash layer. The vegetation restoration of the soil body after the forest fire can also be accelerated by sowing plant seeds. Moreover, the ash layer treated by microorganisms has a certain strength, can resist rainwater scouring, reduce the possibility of it becoming a debris flow source, and can also be utilized by plant growth to accelerate the further fixation of the vegetation on the soil body.

[0083] Therefore, this embodiment is of great significance to the slope surfaces where forest fires occurred before the rainy season and could not be treated in time. Although the microbial-induced precipitation is a rapid and lasting process, it has no pollution to the environment, is simple, rapid and low-cost in operation, and is suitable for various complex environments.

[0084] The specific embodiments described above further elaborate on the purpose, technical solutions and beneficial effects of the present invention. It should be understood that the above description is only the specific embodiments of the present invention and is not used to limit the protection scope of the present invention. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A microbial culture solution, characterized in that, Comprising the following components in parts by weight: Peptone 10 - 20 parts; Glucose 5 - 10 parts; Sodium chloride 5 - 7 parts; Regulating agent 2 - 5 parts, used for regulating the acidity and alkalinity of the culture solution; Buffering agent 1 - 2 parts; Among them, the pH value of the culture solution is 8 - 10.

2. The microbial culture medium according to claim 1, wherein The pH value of the culture solution is 8.5 - 9.

3. The microbial culture medium according to claim 1, wherein The regulating agent is sodium carbonate.

4. The microbial culture medium according to claim 3, wherein The buffering agent is potassium dihydrogen phosphate.

5. The microbial culture medium according to claim 4, wherein The peptone is 10 parts by weight, the glucose is 5 parts by weight, the sodium chloride is 5 parts by weight, the buffering agent is 1 part by weight, and deionized water in 1000 parts by weight is further included.

6. An ash layer solidifying liquid, characterized in that, Comprising the following components in parts by weight: 1000 parts of the microbial culture solution recited in any one of claims 1 - 5; Solidified microorganisms 15 - 25 parts, and the solidified microorganisms are Bacillus subtilis and / or Bacillus kochii; Calcium source 30 - 50 parts.

7. The solidifying liquid for the ash layer according to claim 6, characterized in that, The calcium source is one of calcium chloride, calcium nitrate, and calcium lactate.

8. The solidifying liquid for the ash layer according to claim 7, characterized in that, The solidified microorganisms are 20 parts by weight, and the calcium source is 40 parts by weight.

9. The solidifying liquid for the ash layer according to claim 8, characterized in that, The solidified microorganisms include Bacillus subtilis and Bacillus kochii, and among them, the ratio of Bacillus subtilis to Bacillus kochii is 1:

1.

10. A method for preventing and controlling debris flow after a fire, characterized in that, Spraying the ash layer solidifying liquid recited in any one of claims 6 - 9 on the slope after firing.