Cosmetic composition comprising fermented cordate houttuynia extract

Abstract

The present invention relates to a cosmetic composition comprising a fermented extract of Houttuynia cordata fermented with lactic acid bacteria. The quercetin content of the herba houttuyniae fermentation extract is high, so that the cosmetic composition with physiological and nutritional functions can be obtained. The cosmetic composition containing the herba houttuyniae fermentation extract of the present invention can exhibit excellent skin barrier enhancing, skin moisturizing, antibacterial and regeneration effects.

Description

Technical Field

[0001] The present invention relates to a cosmetic composition containing a Houttuynia cordata fermentation extract fermented with lactic acid bacteria.

[0002] This patent application claims the priority of Korean Patent Application No. 10-2022-0179961, filed on December 21, 2022, and all the contents described in the specification and drawings of this application are incorporated into this application by reference. Background Art

[0003] Houttuynia cordata is a perennial herb of the Saururaceae family, and its scientific name is Houttuynia Cordata. Houttuynia cordata was originally a herb called "Jicao", and it got its name because its leaves have a fishy smell.

[0004] The whole leaves, stems, and roots of Houttuynia cordata contain active ingredients. The physiologically active substances with a unique smell help metabolism, thus purifying the blood and promoting kidney function, and have functions such as relieving inflammation and removing toxins from the body. Therefore, it is widely used as a folk medicine in China, Japan and other countries. In traditional Chinese medicine, Houttuynia cordata is used for hypertension, constipation, diabetes, reducing fever, boils, detoxification, arthritis, inflammatory diseases and skin diseases.

[0005] In this regard, a composition for improving skin wrinkles containing Houttuynia cordata extract is disclosed in Korean Patent Publication No. 10-2022-0091242, and a cosmetic composition for essence water containing Houttuynia cordata extract is disclosed in Korean Patent No. 10-2434747.

[0006] However, it is known that when the Houttuynia cordata extract is directly applied, compared with general chemicals, the effect is significantly weakened. Therefore, a fermentation method etc. for improving the physiological components and their activities of the Houttuynia cordata extract is needed.

[0007] Prior Art Documents

[0008] Patent Documents

[0009] Patent Document 0001: Korean Patent Publication No. 10-2022-0091242 (June 30, 2022)

[0010] Patent Document 0002: Korean Patent Gazette No. 10-2434747 (August 17, 2022) Summary of the Invention

[0011] Technical Problem

[0012] Accordingly, the inventor of the present invention has studied and made efforts to obtain a fermented product of Houttuynia cordata that ensures physiological and nutritional functions. Finally, it was found that the fermented extract of Houttuynia cordata fermented with specific lactic acid bacteria exhibits excellent effects of enhancing skin barrier, skin moisturization, antibacterial, and regeneration, thereby completing the present invention.

[0013] Accordingly, an object of the present invention is to provide a cosmetic composition containing a fermented extract of Houttuynia cordata fermented with lactic acid bacteria.

[0014] Technical Solution

[0015] The present invention provides a cosmetic composition containing a fermented extract of Houttuynia cordata fermented with lactic acid bacteria.

[0016] The lactic acid bacteria may be one or more strains selected from the group consisting of the genus Bacillus, the genus Streptococcus, the genus Lactococcus, the genus Enterococcus, the genus Lactobacillus, the genus Pediococcus, the genus Leuconostoc, the genus Weissella, and the genus Bifidobacterium.

[0017] The lactic acid bacteria may be Bacillus coagulans KK7 (KCTC 19023P).

[0018] The fermented extract of Houttuynia cordata contains quercetin.

[0019] The cosmetic composition may be used for moisturization and enhancing skin barrier.

[0020] On the other hand, the present invention provides a method for preparing a fermented extract of Houttuynia cordata and a fermented extract of Houttuynia cordata prepared by the method. The method includes the step of inoculating Bacillus coagulans KK7 (KCTC 19023P) into Houttuynia cordata and fermenting to obtain a fermented extract of Houttuynia cordata.

[0021] The fermentation may be carried out at a temperature of 20°C to 60°C for 1 day to 5 days.

[0022] Effects of the Invention

[0023] Due to the high content of quercetin in the fermented extract of Houttuynia cordata of the present invention, a cosmetic composition having physiological and nutritional functions can be obtained.

[0024] The cosmetic composition containing the Houttuynia cordata Thunb. fermentation extract of the present invention can exhibit excellent effects of enhancing skin barrier, skin moisturization, antibacterial activity and regeneration. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 It is a chart showing the measurement results of the hyaluronic acid production amount treated with quercetin.

[0026] Figure 2 It is a chart showing the measurement results of the hyaluronic acid production amount treated with the extracts of the examples and comparative examples.

[0027] Figure 3 It is a chart showing the measurement results of the filaggrin production amount treated with quercetin.

[0028] Figure 4 It is a chart showing the measurement results of the filaggrin production amount treated with the extracts of the examples and comparative examples. DETAILED DESCRIPTION OF THE INVENTION

[0029] Hereinafter, the present invention will be described in detail. Before that, the terms or words used in this specification and claims should not be construed as being limited to the ordinary or dictionary meanings, and based on the principle that the inventor can appropriately define the concept of the terms to explain his own invention in the best way, they should be construed as meanings and concepts conforming to the technical idea of the present invention. Therefore, the structure described in the examples in this specification is only one most preferred embodiment of the present invention and does not represent all the technical ideas of the present invention. Therefore, it should be understood that various equivalents and modifications capable of replacing these may exist at the time of filing this application.

[0030] The cosmetic composition of the present invention contains the Houttuynia cordata Thunb. fermentation extract fermented with lactic acid bacteria.

[0031] The Houttuynia cordata Thunb. fermentation extract can be obtained by fermenting the Houttuynia cordata Thunb. extract with lactic acid or fermenting the Houttuynia cordata Thunb. raw material with lactic acid bacteria.

[0032] The Houttuynia cordata Thunb. extract can be extracted using water or C1-C4 alcohols as solvents, but preferably, it can be extracted with hot water.

[0033] The amount of the solvent used can be 5 to 20 times the weight of the Houttuynia cordata Thunb., and preferably, 7 to 15 times the weight can be used. When the amount of the solvent used is less than the defined range, there is a problem that the extraction of the active ingredient is insufficient and the extraction rate is reduced, and when the amount used is greater than the defined range, there is a problem of low efficiency of the extraction process.

[0034] The extraction can utilize solvent extraction methods known in the art such as maceration extraction, ultrasonic extraction, vacuum extraction, etc., and the method is not limited.

[0035] In this case, when hot water is used for the extraction, it can be carried out at a temperature of 40°C to 100°C for 0.5 hours to 24 hours. Preferably, it can be carried out at a temperature of 60°C to 90°C for 1 hour to 12 hours. When the extraction is carried out at a temperature lower than the defined temperature range, the extraction of the active ingredient is insufficient.

[0036] As the lactic acid bacteria, one or more strains selected from the group consisting of the genera Bacillus, Streptococcus, Lactococcus, Enterococcus, Lactobacillus, Pediococcus, Leuconostoc, Weissella, and Bifidobacterium can be used. Preferably, strains of the genera Bacillus, Lactobacillus, Pediococcus, or Lactococcus can be used. More preferably, Bacillus coagulans KK7 (KCTC 19023P) can be used.

[0037] The Houttuynia cordata Thunb. fermentation extract contains quercetin. The quercetin is a flavonoid and is also present in fruits or vegetables. As a powerful antioxidant, it is known to play a very important role in the prevention and treatment of various diseases and is known to have effects such as providing skin nutrition, anti-allergy, anti-inflammatory, and inducing anti-cancer activity through various studies.

[0038] The Houttuynia cordata Thunb. fermentation extract of the present invention shows a higher quercetin content than the general Houttuynia cordata Thunb. extract and can contain about 60 to 180 mg / L of quercetin. Therefore, the present invention can provide a cosmetic composition having moisturizing and skin barrier enhancing effects by promoting the production of hyaluronic acid and filaggrin.

[0039] The cosmetic composition of the present invention can contain ingredients commonly used in cosmetic compositions, for example, it can contain conventional adjuvants and carriers such as antioxidants, stabilizers, co-solvents, vitamins, pigments, and fragrances.

[0040] The cosmetic composition of the present invention can be prepared into any dosage form commonly prepared in the art. For example, it can be formulated into solutions, suspensions, emulsions, ointments, gels, creams, lotions, powders, soaps, surfactant-containing cleaners, oils, powder foundations, emulsion foundations, wax foundations, and sprays, etc., but not limited thereto.

[0041] More specifically, it can be formulated into a soft lotion, a nourishing lotion, a nourishing cream, a massage cream, an essence, an eye cream, a cleansing balm, a facial cleansing foam, a makeup remover, a facial mask, a spray or a powder dosage form.

[0042] When the dosage form of the present invention is an ointment, a cream or a gel, animal oils, vegetable oils, waxes, paraffins, starches, tragacanth, cellulose derivatives, polyethylene glycols, silicones, bentonites, silica, talc or zinc oxide, etc. can be used as carrier components.

[0043] When the dosage form of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder can be used as carrier components. In particular, when it is a spray, propellants such as chlorofluorocarbons, propane / butane or dimethyl ether can be additionally included.

[0044] When the dosage form of the present invention is a solution or an emulsion, solvents, cosolvents or emulsifiers are used, for example, including water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3 - butanediol oil, fatty acid esters of glycerides, polyethylene glycol or fatty acid esters of sorbitan.

[0045] When the dosage form of the present invention is a suspension, liquid diluents such as water, ethanol or propylene glycol can be used; suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitan esters and polyoxyethylene dehydrated sorbitol esters; and carrier components such as microcrystalline cellulose, metahydroxyaluminum, bentonite, agar or tragacanth.

[0046] When the dosage form of the present invention is a surfactant - containing cleanser, fatty alcohol sulfates, fatty alcohol ether sulfates, sulfosuccinic acid monoesters, hydroxyethyl sulfonates, imidazoline derivatives, methyl taurine, sarcosinates, fatty acid amide ether sulfates, alkyl amide betaines, fatty alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters, etc. can be used as carrier components.

[0047] When the cosmetic composition of the present invention is a soap, a surfactant - containing cleanser or a surfactant - free cleanser, it can be wiped, removed or washed with water after being applied to the skin. As specific examples, the soap is a liquid soap, a powder soap, a solid soap and an oil soap, the surfactant - containing cleanser is a facial cleansing foam, a makeup remover, makeup remover wipes and a cleansing facial mask, and the surfactant - free cleanser is a cleansing balm, a makeup remover milk, a makeup remover and a makeup remover jelly, but not limited thereto.

[0048] Moreover, the houttuynia fermentation extract can be included in a skin - external pharmaceutical composition, and the pharmaceutical composition can be a cream, a gel, a patch, a spray, an ointment, a plaster, a lotion, a liniment, a paste or a poultice, but not limited thereto.

[0049] On the other hand, the present invention provides a method for preparing a fermented extract of Houttuynia cordata, the method comprising the step of inoculating Bacillus coagulans KK7 (KCTC 19023P) into Houttuynia cordata and fermenting to obtain a fermented extract of Houttuynia cordata.

[0050] In this case, the inoculation can be carried out with an extract of Houttuynia cordata, and the extract of Houttuynia cordata is preferably a hot water extract, but is not limited thereto, and the inoculation can be carried out with raw Houttuynia cordata.

[0051] Relative to the weight of the extract of Houttuynia cordata or raw Houttuynia cordata, the strain of Bacillus coagulans KK7 (KCTC 19023P) can be inoculated at a concentration of 1% to 10%, preferably 3% to 7%. When the inoculation concentration of the lactic acid bacteria is less than 1%, it may cause insufficient fermentation, and when it is greater than 10%, it may cause over-fermentation.

[0052] The fermentation can be carried out at a temperature of 20°C to 60°C for 1 day to 10 days. When the fermentation is carried out below the above range, it may cause insufficient fermentation, and when it is carried out above the above range, it may cause over-fermentation.

[0053] The fermentation can be carried out at a temperature of 20°C to 60°C for 1 day to 5 days, preferably at a temperature of 30°C to 40°C for 3 days to 4 days. When the fermentation is carried out below the above range, it may cause insufficient fermentation, and when it is carried out above the above range, it may cause over-fermentation.

[0054] Hereinafter, the present invention will be described in detail with reference to examples and experimental examples to specifically illustrate the present invention. However, the examples of the present invention can be changed into various different forms, and the scope of the present invention should not be construed as being limited to the following examples. The examples of the present invention are provided to more completely illustrate the present invention to those of ordinary skill in the art.

[0055] Example: Preparation of a fermented extract of Houttuynia cordata by lactic acid bacteria

[0056] Suspend 1 g of the kimchi sample in sterile physiological saline and then dilute to 10 -6To prepare a suspension solution. Then, 100 μL of the suspension solution was inoculated onto BDTM Tryptic Soy Agar (15 g·L-1 pancreatic digest of casein, 5 g·L-1 papaic digest of soybean meal, 5 g·L-1 sodium chloride, 5 g·L-1 agar; Product No 236950) medium and spread. Single colonies were subcultured in the medium under dark conditions at 30 ± 1 °C and pH 7.3 ± 0.2 for 48 hours to screen and purify the isolated microbial strains, and were finally identified as Bacillus coagulans KK7 by 16S-rRNA base sequence analysis. The Bacillus coagulans KK7 strain was deposited at the Biological Resource Center of the Korea Research Institute of Bioscience and Biotechnology on July 27, 2022, with the deposit number KCTC 19023P.

[0057] The screened and isolated strain was subcultured in Tryptic Soy Agar medium at intervals of 24 hours under dark conditions at 30 ± 1 °C and pH 7.3 ± 0.2.

[0058] Next, 10 times the volume of pure water was added to dried Houttuynia cordata, and then hot water extraction was carried out at a temperature of 80 °C for 60 minutes to obtain an extract solution removing Houttuynia cordata. The cultured Bacillus coagulans KK7 strain was inoculated into the extract solution at 5.0% of the extract weight, and fermented at a temperature of 35 °C for 3 days, and then sterilized to obtain a Houttuynia cordata fermented extract. The filtered extract solution was concentrated under reduced pressure and completely dehydrated using a freeze dryer, and then an extract powder was obtained and used for experiments.

[0059] Comparative Example 1: Preparation of Houttuynia cordata extract

[0060] 10 times the volume of pure water was added to dried Houttuynia cordata, and then hot water extraction was carried out at a temperature of 80 °C for 60 minutes to obtain an extract solution removing Houttuynia cordata. Then, the extract solution was concentrated under reduced pressure and completely dehydrated using a freeze dryer, and then an extract powder was obtained and used for experiments.

[0061] Experimental Example 1: Quercetin content analysis

[0062] In order to compare the physiological components of the extracts of the said examples and comparative examples, the index components were analyzed. The quercetin content of the extracts of the said examples and comparative examples was confirmed by HPLC analysis using a Shim-pack GIS C18 column. 100 mL of the extraction solutions of the said examples and comparative examples were respectively taken, and 200 mL of ethyl acetate was added thereto for liquid-liquid extraction. Then, the ethyl acetate layer was recovered and concentrated under reduced pressure at a water bath temperature of about 30°C. 10 mL of methanol was added to the completely concentrated sample and dissolved, and then it was filtered with a syringe filter and the quercetin content was measured. The standard product was prepared by dissolving it in methanol so that the concentration was 0.5 to 200 μg / mL, and then it was filtered with a syringe filter and used. After measurement, the peak area was calculated, and a standard calibration curve was constructed using the regression equation. The mobile phase used distilled water containing 0.1% trifluoroacetic acid and acetonitrile, the flow rate was 1.0 mL / minute, the detector used a PDA detector (371 nm), the elution conditions were in gradient mode, and the ratio of acetonitrile was changed within 60 minutes for measurement.

[0063] Table 1

[0064] Sample Name Quercetin Content (mg / L) S.D Example 123.03 0.10 Comparative Example 31.69 0.04

[0065] As shown in Table 1 above, it was shown that the quercetin content of the Houttuynia cordata fermentation extract of the example was very high, and thus it was confirmed that the content of quercetin was increased by lactic acid bacteria fermentation.

[0066] Experimental Example 2: Culturing skin keratinocytes

[0067] HaCaT cells as skin keratinocytes were purchased from Cell Line Service GmbH. (Germany). They were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) medium containing 1% penicillin-streptomycin and 10% fetal bovine serum (FBS) in an incubator at 37°C and 5% CO2, and subcultured at intervals of 2 to 3 days.

[0068] Experimental Example 3: Cytotoxicity evaluation

[0069] EZ-Cytoxassay

[0070] The EZ-cytox assay is a representative method for measuring cell viability based on the principle that water-soluble tetrazolium salt (WST) reacts with the dehydrogenase of living cells to generate orange water-soluble formazan.

[0071] To confirm cytotoxicity, HaCaT cells were seeded at 1.0×10 4 cells / well in a 96-well plate and cultured in DMEM medium supplemented with 10% FBS at 37 °C and 5% CO2 for 24 hours. The medium of the cultured cells was replaced with serum-free DMEM and treated with the sample to be evaluated. Then, EZ-cytox was added to each well and reacted at 37 °C and 5% CO2 for 30 minutes, and then the absorbance at 450 nm was measured using a microplate reader. The average absorbance value of each sample group was calculated and compared with the absorbance value of the control group to evaluate cell viability.

[0072] Table 2

[0073]

[0074] As shown in Table 2, no cytotoxicity was observed in the examples, comparative examples, and quercetin in keratinocytes.

[0075] Experimental Example 4: Effect of increasing the production of moisturizing factor hyaluronic acid (HA)

[0076] HaCaT cells were seeded at 1.0×10 5 cells / well in a 24-well plate and cultured at 37 °C and 5% CO2 for 24 hours. The medium of the cultured cells was replaced with serum-free DMEM medium, and then treated with different concentrations of the sample and cultured for 24 hours. Then, the supernatant of each experimental group was taken and the amount of hyaluronic acid (HA) produced in the medium was measured. The control group was treated with N-acetylglucosamine at a concentration of 10 mM. The measurement of the amount of hyaluronic acid produced was performed using a Hyaluronan Quantikine ELISA kit (R&D System) according to the method provided by the manufacturer. Figure 1 The results of measuring the production of hyaluronic acid treated with quercetin are shown,Figure 2 The measurement results of the hyaluronic acid production amount treated with the extracts of the examples and comparative examples are shown.

[0077] As Figure 1 shown, quercetin promotes the production of hyaluronic acid as a moisturizing factor. In particular, as Figure 2 shown, it can be confirmed that the fermented extract of the example promotes the production of hyaluronic acid as a moisturizing factor more than the extract of the comparative example.

[0078] Experimental Example 5: Effect of increasing the production amount of the moisturizing factor filaggrin (FLG)

[0079] HaCaT cells were seeded in a 24-well plate at 1.0×10 5 cells / well and cultured at 37 °C and 5% CO2 for 24 hours. The medium of the cultured cells was replaced with serum-free DMEM medium, and then treated with the samples at different concentrations and cultured for 24 hours. Then, the cells of each experimental group were lysed, the proteins were collected, and the production amount of filaggrin in the culture medium extract was measured. The measurement of the filaggrin production amount was performed using a filaggrin ELISA kit (Cusabio Biotechnology Co., Ltd.) according to the method provided by the manufacturer. Figure 3 The measurement results of the filaggrin production amount treated with quercetin are shown, Figure 4 The measurement results of the filaggrin production amount treated with the extracts of the examples and comparative examples are shown.

[0080] As Figure 3 shown, quercetin promotes the production of filaggrin as a moisturizing factor. In particular, as Figure 4 shown, it can be confirmed that the fermented extract of the example promotes the production of filaggrin as a moisturizing factor more than the extract of the comparative example.

Claims

1. A cosmetic composition, characterized in that, Contains a houttuynia cordata fermentation extract fermented with lactic acid bacteria.

2. The cosmetic composition according to claim 1, characterized in that, The lactic acid bacteria are one or more strains selected from the group consisting of the genera Bacillus, Streptococcus, Lactococcus, Enterococcus, Lactobacillus, Pediococcus, Leuconostoc, Weissella, and Bifidobacterium.

3. The cosmetic composition according to claim 2, characterized in that, The lactic acid bacteria are Bacillus coagulans KK7, and its deposit number is KCTC 19023P.

4. The cosmetic composition according to claim 1, characterized in that, The houttuynia cordata fermentation extract contains quercetin.

5. The cosmetic composition according to claim 1, characterized in that, The composition has excellent moisturizing and skin barrier enhancing effects.

6. A preparation method of a houttuynia cordata fermentation extract, characterized in that, Includes the step of inoculating Bacillus coagulans KK7 with a deposit number of KCTC 19023P into houttuynia cordata and fermenting to obtain a houttuynia cordata fermentation extract.

7. The preparation method of the houttuynia cordata fermentation extract according to claim 6, characterized in that, The fermentation is carried out at a temperature of 20 °C to 60 °C for 1 day to 5 days.

8. A fermented extract of Houttuynia cordata, characterized in that, Prepared by the method for preparing a houttuynia cordata fermentation extract according to claim 6.

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