Pesticide composition for preventing and treating tomato bacterial wilt

Abstract

The invention belongs to the technical field of pesticides, and particularly relates to a pesticide composition for preventing and treating tomato bacterial wilt. The invention relates to a sterilization composition, which is formed by compounding zhongshengmycin and copper citrate, or is formed by compounding zhongshengmycin and cuaminosulfate. When the Zhongshengmycin is compounded with the existing medicament copper citrate or cuaminosulfate, the Zhongshengmycin shows a synergistic effect on ralstonia solanacearum causing tomato bacterial wilt, and compared with the single use of the Zhongshengmycin, the compound formula disclosed by the invention can improve the prevention and treatment effect on the tomato bacterial wilt, and also contributes to improving the utilization rate of the existing medicament.

Description

Technical Field

[0001] The present invention belongs to the technical field of pesticides, and particularly relates to a pesticide composition for controlling tomato bacterial wilt. Background Art

[0002] Tomato is an important economic crop, rich in substances such as vitamin A, lycopene, and carotenoids. Research shows that regular consumption of tomatoes and their products is beneficial to human health and can effectively prevent diseases such as inflammation.

[0003] Tomato production ranks first in China's protected agriculture. Although great progress has been made in tomato cultivation technology, disease problems frequently lead to yield reduction. Tomato bacterial wilt caused by Ralstonia solanacearum is an important soil-borne disease in tomato production. In the initial stage of the disease, the leaves wilt during the day and recover at night, and the color changes from dark to light. After the plant completely wilts and dies, its vascular bundles change color, and white mucus will ooze out when the stem is squeezed, and the bacterial wilt can be isolated from it. The phenomenon of dead plants is obvious, resulting in a decline in tomato yield and quality.

[0004] In production, Zhongshengmycin can be used to control tomato bacterial wilt. However, with the long-term use of Zhongshengmycin, the resistance of the pathogen gradually accumulates, resulting in unsatisfactory control effect. Increasing the dosage will exacerbate the development of resistance and also cause problems such as drug residues and ecological environment damage. Mixing or compounding different bactericidal active ingredients is an effective and convenient means to improve the control effect. Therefore, studying the compounding or mixing of Zhongshengmycin with existing pesticides is of great significance for improving the control effect of tomato bacterial wilt.

[0005] Copper citrate, CAS: 10402-15-0, molecular formula: C6H4Cu2O7·2.5H2O, relative molecular weight: 360.22. The prior art has disclosed that a bactericidal composition containing bronopol and copper citrate has high activity against a series of bacterial diseases on various crops and has a significant synergistic effect.

[0006] Ammoniacal copper has good control effects on both fungal and bacterial diseases, and at the same time promotes crop growth and increases crop yield. Its mechanism of action is that through the exchange of copper ions with cations such as K + and H + on the cell membrane surface of the pathogen, the proteins on the cell membrane of the pathogen coagulate, and at the same time, some copper ions penetrate into the pathogen cell and combine with certain enzymes to affect their activity. The prior art has disclosed that a compound composition containing metconazole and ammoniacal copper has an obvious synergistic effect on controlling tomato bacterial wilt.

[0007] Currently, there is no relevant report on the compounding of Zhongshengmycin with copper citrate or ammoniacal copper.

[0008] The information disclosed in this background section is only intended to enhance the overall understanding of the background of the present invention and should not be regarded as an admission or any form of implication that this information constitutes prior art already known to those of ordinary skill in the art. Summary of the Invention

[0009] The object of the present invention is to provide a pesticide composition for controlling tomato bacterial wilt to solve the problems of the prior art in the background art.

[0010] To achieve the above object, the present invention provides the following technical solutions:

[0011] A bactericidal composition, which is composed of zhongshengmycin and copper citrate in a compound form, or composed of zhongshengmycin and copper ammonia complex in a compound form.

[0012] Further, the compound mass ratio of zhongshengmycin to copper citrate is 1 - 5:20 - 1.

[0013] Further, the compound mass ratio of zhongshengmycin to copper ammonia complex is 1 - 10:30 - 1.

[0014] The present invention also provides a bactericide, which includes an active ingredient and an auxiliary ingredient; wherein, the active ingredient is the above-mentioned bactericidal composition.

[0015] Further, the mass of the active ingredient accounts for 0.25 - 75% of the total mass of the bactericide.

[0016] Further, the mass of the active ingredient accounts for 8 - 55% of the total mass of the bactericide.

[0017] The present invention also provides the application of the above-mentioned bactericidal composition or the above-mentioned bactericide in controlling tomato bacterial wilt.

[0018] Further, the tomato bacterial wilt is caused by the infection of Ralstonia solanacearum.

[0019] Compared with the prior art, the present invention has the following beneficial effects:

[0020] (1) When the present invention compounds zhongshengmycin with the existing agents copper citrate or copper ammonia complex, it shows a synergistic effect on Ralstonia solanacearum that causes tomato bacterial wilt. Compared with using zhongshengmycin alone, the compound formulation of the present invention can improve the control effect on tomato bacterial wilt and at the same time help to improve the utilization rate of the existing agents.

[0021] (2) The compound formulation of the present invention can delay the accumulation of pathogen drug resistance and is of great significance for the comprehensive management of tomato bacterial wilt drug resistance. Detailed Embodiments

[0022] The following is a clear and complete description of the technical solution of this invention patent. Obviously, the described embodiments are part of the embodiments of the present invention, rather than all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative work belong to the scope of protection of the present invention.

[0023] Example: Indoor bioactivity test after compounding Zhongshengmycin

[0024] 1. Test pathogens

[0025] Ralstonia solanacearum, isolated from diseased tomato samples with bacterial wilt.

[0026] 2. Culture media

[0027] NA medium: Beef extract 5 g, yeast extract 1 g, peptone 5 g, sucrose 5 g, agar 15 g, made up to 1 L with distilled water and adjusted to pH 7.0 with 10 mol / L NaOH.

[0028] NB medium: Beef extract 5 g, yeast extract 1 g, peptone 5 g, sucrose 5 g, made up to 1 L with distilled water and adjusted to pH 7.0 with 10 mol / L NaOH.

[0029] 3. Test agents

[0030] 99% Zhongshengmycin (Guangdong Wengjiang Chemical Reagent Co., Ltd.), 99.5% copper citrate (Shanghai Yuanye Bio-Technology Co., Ltd.), 25% copper ammonia complex aqueous solution (Shanghai Lvze Biotechnology Co., Ltd.).

[0031] After dissolving Zhongshengmycin and copper citrate, they were diluted into single-agent mother liquors with 0.1% Tween-80 aqueous solution; the copper ammonia complex aqueous solution was directly diluted into a single-agent mother liquor with 0.1% Tween-80 aqueous solution. Multiple groups of ratios were set, and each single agent and each group of mixed agents were further diluted into 5 series of mass concentration gradients with 0.1% Tween-80 aqueous solution for standby.

[0032] 4. Test method (refer to "NT / T 1156.16-2008 Guidelines for Pesticide Indoor Bioassay Tests Fungicides Part 16: Turbidity Method for Inhibiting Bacterial Growth Amount Test").

[0033] The test strain Ralstonia solanacearum was inoculated onto NA medium and cultured overnight at 30°C. Single colonies were picked and inoculated into NB medium, and cultured at 30°C with shaking at 180 r / min until the OD 600 value reached 0.8, and then the concentration of the bacterial suspension was adjusted to 1.0×10 8 cfu / mL.

[0034] Absorb 1 mL of the medicinal liquid and add it to 50 mL of NB medium. Set the treatment of adding 1 mL of 0.1% Tween-80 aqueous solution to 50 mL of NB medium as the blank control, and set 5 replicates for each treatment. Then inoculate 150 μL of the test pathogen suspension into each treatment. After measuring and recording the turbidity before cultivation of each treatment, transfer it to an oscillating culture at 30 °C and 180 r / min. When the OD 600 value of the test pathogen in the blank control treatment reaches 0.8, measure the turbidity of each treatment again, and calculate the growth inhibition rate of the test pathogen.

[0035]

[0036] 4. Data analysis

[0037] Perform linear regression analysis with the logarithm of the drug concentration as x and the probit value of the corresponding growth inhibition rate as y, calculate the EC 50 of the drug for each treatment, and calculate the co-toxicity coefficient (CTC value) of the mixture according to the Sun Yunpei method.

[0038]

[0039] In the above formula: ATI—the measured toxicity index of the mixture; S—the LC 50 of the standard drug, unit: mg / L; M—the LC 50 of the mixture, unit: mg / L.

[0040] TTI = TI A × P A + TI B × P B

[0041] In the above formula: TTI—the theoretical toxicological index of the mixture; TI A —the toxicity index of drug A; P A —the percentage content of drug A in the mixture, unit: percentage (%) ; TI B —the toxicity index of drug B; P B —the percentage content of drug B in the mixture, unit: percentage (%).

[0042]

[0043] In the above formula: CTC—co-toxicity coefficient; ATI—measured toxicity index of the mixture; TTI—theoretical toxicity index of the mixture.

[0044] 5. Efficacy evaluation

[0045] The synergistic effect of mixed drugs was evaluated based on the co-toxicity coefficient (CTC), that is, CTC ≤ 80 was antagonistic, 80 < CTC < 120 was additive, and CTC ≥ 120 was synergistic. The results are shown in Table 1-2.

[0046] Table 1 Indoor bioactivity test of the complex of zhongshengmycin and copper citrate against Ralstonia solanacearum

[0047] Names and ratios of medicaments <![CDATA[EC 50 (mg / L)]]> ATI TTI CTC Zhongshengmycin 9.041 100.000 -- -- Copper citrate 64.725 13.968 -- -- Zhongshengmycin 1: Copper citrate 20 37.258 24.266 18.065 134.325 Zhongshengmycin 1: Copper citrate 10 29.197 30.966 21.789 142.113 Zhongshengmycin 1: Copper citrate 5 24.430 37.008 28.307 130.737 Zhongshengmycin 1: Copper citrate 3 16.200 55.809 35.476 157.313 Zhongshengmycin 1: Copper citrate 1 9.708 93.129 56.984 163.430 Zhongshengmycin 3: Copper citrate 1 6.459 139.975 78.492 178.330 Zhongshengmycin 5: Copper citrate 1 7.936 113.924 85.661 132.993

[0048] As shown in Table 1, when Zhongshengmycin and copper citrate were compounded at a mass ratio of 1-5:20-1, the co-toxicity coefficient against Ralstonia solanacearum, which infects tomato plants, was greater than 120, demonstrating a synergistic effect. This indicates that the combination of Zhongshengmycin and copper citrate in the present invention can improve the control of tomato bacterial wilt compared to using Zhongshengmycin alone.

[0049] Table 2 Indoor bioactivity test of Zhongshengmycin and copper chlorammine complex against Ralstonia solanacearum

[0050] Names and ratios of medicaments <![CDATA[EC 50 (mg / L)]]> ATI TTI CTC Zhongshengmycin 9.041 100.000 -- -- Amino copper complex 81.409 11.106 -- -- Zhongshengmycin 1: Amino copper complex 30 41.782 21.639 13.973 154.857 Zhongshengmycin 1: Amino copper complex 20 34.651 26.092 15.339 170.103 Zhongshengmycin 1: Amino copper complex 10 19.434 46.522 19.187 242.465 Zhongshengmycin 1: Amino copper complex 5 11.026 81.997 25.921 316.330 Zhongshengmycin 1: Amino copper complex 1 13.222 68.378 55.553 123.087 Zhongshengmycin 5: Amino copper complex 1 4.294 210.550 85.184 247.170 Zhongshengmycin 10: Amino copper complex 1 5.568 162.374 91.919 176.650

[0051] As shown in Table 2, when Zhongshengmycin and copper comminuted are combined in a mass ratio of 1-10:30-1, the co-toxicity coefficient against Ralstonia solanacearum, which infects tomato plants, is greater than 120, demonstrating a synergistic effect. This indicates that the combination of Zhongshengmycin and copper comminuted in the present invention can improve the control of tomato bacterial wilt compared to using Zhongshengmycin alone.

[0052] In summary, the present invention's combination of Zhongshengmycin with existing agents, copper citrate or copper chelate, exhibits a synergistic effect against Ralstonia solanacearum, the pathogen that causes tomato bacterial wilt. Compared to using Zhongshengmycin alone, the present invention's combined formulation improves the control of tomato bacterial wilt while also helping to increase the utilization rate of existing agents. Furthermore, the present invention's combined formulation can slow the accumulation of drug resistance in pathogens, significantly contributing to the comprehensive management of drug resistance in tomato bacterial wilt.

Claims

1. A bactericidal composition, characterized in that, The bactericidal composition is prepared by compounding Zhongshengmycin with copper citrate, or by compounding Zhongshengmycin with ammoniated copper.

2. The bactericidal composition according to claim 1, wherein The mass ratio of compounding Zhongshengmycin with copper citrate is 1-5:20-1.

3. The bactericidal composition according to claim 1, characterized in that, The mass ratio of compounding Zhongshengmycin with ammoniated copper is 1-10:30-1.

4. A fungicide, characterized in that, The fungicide comprises an active ingredient and an auxiliary ingredient; wherein, the active ingredient is the bactericidal composition as described in claim 1.

5. The fungicide according to claim 1, characterized in that, The mass of the active ingredient accounts for 0.25-75% of the total mass of the fungicide.

6. The fungicide according to claim 1, characterized in that, The mass of the active ingredient accounts for 8-55% of the total mass of the fungicide.

7. Use of the bactericidal composition according to any one of claims 1-3 or the fungicide according to any one of claims 4-6 in controlling tomato bacterial wilt.

8. The application according to claim 7, wherein The tomato bacterial wilt is caused by infection of Ralstonia solanacearum.

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