Preparation method and application of compound essential oil efficacy core ball steady-state delivery system and essence emulsion thereof

Abstract

The present invention discloses a composite essential oil efficacy core ball steady-state delivery system and a preparation method and application of its essence emulsion, belonging to the field of cosmetic technology. The present invention innovatively develops a composite essential oil efficacy core ball steady-state delivery system of "unstable small molecule active ingredient + biosynthetic active macromolecule + penetration enhancer molecule + functional small molecule + essential oil", which has an excellent spherical appearance, a smooth spherical surface, excellent solubility and dispersibility, and a porosity of more than 90%; when it is dispersed into the essence oil, the essence oil quickly fills the pores of the efficacy core ball to obtain the composite essential oil efficacy core ball. At the same time, the present invention also develops a full-process low-temperature production and processing technology based on liquid nitrogen quick freezing to ensure that the activity of the small molecule active ingredient is not affected by the process and to ensure the high-quality production of the efficacy core ball.

Description

Technical Field

[0001] The invention relates to a composite essence oil efficacy core ball steady-state delivery system and a preparation method and application of an essence emulsion thereof, belonging to the technical field of cosmetics. Background Art

[0002] Functional active ingredients are widely used in the cosmetics, health food and other industries, but many active ingredients have problems such as instability, odor, poor compatibility, and low bioavailability, which seriously restrict their application in cosmetics and food.

[0003] In water-based formula products, such as face creams, eye creams, essences, beverages, etc., the stability, compatibility and utilization of active ingredients face greater challenges, especially the problem of biological activity decay during the shelf life. Many active ingredients almost lose their activity within a shelf life of 1-3 years.

[0004] Therefore, it is necessary to solve these pain points through advanced steady-state delivery technology and innovative product design, so as to promote the product upgrading and innovative development of cosmetics and health foods. Summary of the Invention

[0005] In order to overcome the above technical defects, the present invention has developed a composite essential oil efficacy core ball steady-state delivery system and its essence preparation method and application, which successfully solves the pain point problem of unstable application.

[0006] The composite freeze-dried functional core ball steady-state delivery system of the present invention has an excellent spherical appearance, a smooth spherical surface, outstanding solubility and dispersibility, and a porosity of more than 90%. When it is dispersed into essential oil, the essential oil quickly fills the pores of the functional core ball, thereby obtaining a composite essential oil functional core ball.

[0007] First, we innovatively developed a compound essential oil efficacy core ball steady-state delivery system consisting of "unstable small molecule active ingredients + biosynthetic active macromolecules + penetration enhancer molecules + functional small molecules + essential oils." These small molecule active ingredients, active macromolecules, penetration enhancer molecules, and functional small molecules interact with each other through multiple intermolecular interactions to form an orderly and stable nano-assembly stabilized structure system, thus solving the stability and compatibility issues of small molecule active ingredients.

[0008] Secondly, we developed a skincare essence oil. When the functional core balls are dispersed in the essence oil, the essence oil quickly fills the pores of the functional core balls, resulting in a composite essential oil functional core ball. This further improves the stability of the active ingredients, the mechanical strength of the functional core ball, and its shelf stability.

[0009] Thirdly, penetration enhancer molecules, such as phloretin, cyclodextrin, and menthol, can further enhance the transdermal absorption rate of small molecules. Fourthly, a solvent solution is developed. By mixing the solvent solution with the essence oil core ball and gently shaking to mix evenly, a freshly prepared functional skin care essence lotion is obtained.

[0010] Finally, a full-process low-temperature production and processing technology based on liquid nitrogen quick freezing was developed to ensure that the activity of small molecule active ingredients is not affected by the process and to ensure high-quality production of functional core balls.

[0011] The composite essential oil efficacy core ball steady-state delivery system of the present invention comprises: unstable small molecule active ingredients, biosynthetic active macromolecules, penetration enhancer molecules, functional small molecules and essential oil.

[0012] In the above-mentioned compound essence oil efficacy core ball steady-state delivery system, small molecule active ingredients, active macromolecules, penetration enhancer molecules and functional small molecules form an orderly and stable nano-assembly steady-state structure system through multiple intermolecular interactions, successfully solving the application pain points of small molecule active ingredients such as stability, compatibility and absorbability.

[0013] Furthermore, in the above technical solution, the unstable small molecule active ingredients include: ergothioneine, glutathione, anthocyanin, blue copper peptide, vitamin C (hereinafter referred to as Vc), ethylbisiminomethyl elm alkaloid manganese chloride (hereinafter referred to as EUK134) and other active ingredients. One or more.

[0014] Furthermore, in the above technical solution, the content of the unstable small molecule active ingredient (the content described in the present invention refers to the weight content, the same below) is 0.1%-70%, more preferably 1%-30%, and even more preferably 5%-20%;

[0015] In the above-mentioned compound essence oil functional core ball steady-state delivery system, the biosynthetic active macromolecule is used to construct the skeleton of the functional core ball to improve the mechanical stability of the functional core ball.

[0016] Furthermore, in the above technical solution, the biosynthetic active macromolecules include: fermented polysaccharides, fermented proteins, recombinant collagen, recombinant type III collagen, recombinant type XII collagen, recombinant fibronectin, yeast protein, polyglutamic acid, pullulan, yeast polysaccharide, yeast β-glucan, sodium hyaluronate, xanthan gum, gellan gum, sodium DNA (PDRN polydeoxyribonucleotide), fermented Ganoderma lucidum polysaccharide and other ingredients or one or more.

[0017] Furthermore, in the above technical solution, the molecular weight of the active macromolecule synthesized by biological fermentation is in the range of 1,000-2,000,000, more preferably in the range of 3,000-1,200,000, and even more preferably in the range of 10,000-800,000.

[0018] In the above-mentioned composite essence oil efficacy core ball steady-state delivery system, the penetration enhancer molecule can further improve the transdermal absorption efficiency of small molecule active ingredients.

[0019] Furthermore, in the above technical solution, the penetration enhancer molecules include: one or more of phloretin, menthol, hydroxypropyl β-cyclodextrin, methyl β-cyclodextrin, butylene glycol, etc.

[0020] In the above-mentioned composite essence oil functional core ball steady-state delivery system, the functional small molecules can further enrich or improve the skin care efficacy of the functional core ball.

[0021] Furthermore, in the above technical solution, the functional small molecules include one or more active ingredients such as hydroxy-madecassoside, asiaticoside, theanine, swalnic acid, arbutin, nicotinamide, tranexamic acid, ectoine, Vc glucoside, catechin, trehalose, nonapeptide-1, snake venom peptide, cono-like peptide, acetyl hexapeptide-8, and mannitol.

[0022] In the above-mentioned composite essence oil functional core ball steady-state delivery system, the functional core balls are dispersed in the essence oil, which further improves the stability of the active ingredients, the mechanical strength of the functional core balls, and the shelf stability.

[0023] Furthermore, in the above technical solution, the essential oil includes: coconut oil-caprylate / caprate, isohexadecane, isododecane, jojoba seed oil, squalane, fermented peony seed oil, fermented sacha inchi oil, fermented rice bran oil, fermented grape seed oil, fermented vegetable oil, serrata australis seed oil, corn germ oil, tocopheryl acetate, rapeseed oil, macadamia seed oil, isononyl isononanoate, ethylhexyl palmitate, caprylic / capric triglyceride, white meadowfoam seed oil, sunflower seed oil, damascena flower oil, hydrogenated polyisobutene, polydimethylsiloxane, lavender essential oil, tea tree essential oil, plant essential oil, essence, etc. One or more thereof.

[0024] Furthermore, in the above technical solution, the mass ratio of the unstable small molecule active ingredient, biosynthetic active macromolecule, penetration enhancer molecule and functional small molecule is 1:0.1-10:0.05-5:0.1-10:1000-10000, and a more suitable ratio range is 1:0.5-5:0.1-1:0.5-5:100-1000.

[0025] Furthermore, in the above technical solution, the particle size of the composite essence oil efficacy core ball is in the range of 0.1-15 mm, more preferably in the range of 0.5-8 mm; and even more preferably in the range of 1-5 mm.

[0026] The present invention also provides a production process for the aforementioned composite essential oil efficacy core ball steady-state delivery system. This process utilizes a full-process low-temperature process involving liquid nitrogen quick freezing, ensuring that the activity of small molecule active ingredients is not affected by the process. The production process for the composite essential oil efficacy core ball steady-state delivery system comprises the following steps: first, preparing composite freeze-dried efficacy core balls; then, dispersing the efficacy core balls in essential oil, allowing the essential oil to rapidly fill the pores of the efficacy core balls, thereby producing the composite essential oil efficacy core balls.

[0027] The production process of the composite essential oil efficacy core ball steady-state delivery system of the present invention comprises the following specific steps:

[0028] A. Mix the unstable small molecule active raw material and the biosynthetic active macromolecule evenly, then add purified water and stir to dissolve to obtain a clear and transparent solution;

[0029] B. During the stirring process, the penetration enhancer molecules and the functional small molecules are added to the above solution and stirred until the various components are completely dissolved to obtain the target solution;

[0030] C. Add the target solution dropwise into a heat-insulated liquid nitrogen bucket. During the addition, nitrogen gas needs to be introduced into the target solution to prevent the active ingredient from being oxidized and lost.

[0031] D. freeze-dry the small ice balls formed by freezing liquid nitrogen to obtain composite freeze-dried functional core balls.

[0032] E. Disperse the functional core balls into the essential oil, and the essential oil quickly fills the pores of the functional core balls to obtain a composite essential oil functional core ball steady-state delivery system.

[0033] Furthermore, in the above technical solution, during the dropwise addition in step C, a dropwise addition device including but not limited to a peristaltic pump, a syringe pump, a flow pump, etc. that can control the speed and droplet size is used.

[0034] Furthermore, in the above technical solution, the preparation process of the essential oil in step E is as follows: weigh different oil components separately, put them into a container in turn, and stir them evenly until they are clear and transparent to obtain the (skin care) essential oil.

[0035] The present invention also provides a method for preparing a functional skin care essence, comprising the following steps: mixing a solvent liquid with the aforementioned essential oil functional core ball steady-state delivery system, gently shaking and mixing to obtain a freshly prepared functional skin care essence.

[0036] Furthermore, in the above technical solution, the solvent liquid of the present invention includes water, a humectant, and / or an emulsifier. The solvent liquid preparation process is briefly as follows: the humectant component and the emulsifier component are weighed separately, placed in a container in order, and then purified water is added and stirred until clear and transparent to obtain the solvent liquid.

[0037] Furthermore, in the above technical solution, the moisturizing agent includes: xylitol glucoside, anhydroxylitol, xylitol, Tremella polysaccharide, betaine, sodium hyaluronate, xanthan gum, hydroxyethyl cellulose, butylene glycol, pentylene glycol, hexylene glycol, parahydroxyacetophenone, glycerin, β-glucan, polyglycerol-10, glyceryl polyether-26, wrinkled chondrus crispus, glycosyl trehalose, hydrogenated starch hydrolysate, etc. One or more thereof.

[0038] Furthermore, in the above technical solution, the emulsifier includes: Tween-20 (polysorbate 20), Tween 80, laureth-4, PEG-40 hydrogenated castor oil, soybean seed extract, coconut glucoside, PEG stearate, etc. BRIEF DESCRIPTION OF THE DRAWINGS

[0039] Figure 1 Diagrams of the steady-state delivery system of A1, A2, and A3 ergothioneine composite freeze-dried efficacy core balls and B1, B2, and B3 ergothioneine composite essential oil efficacy core balls. The samples are all from Examples 1-3.

[0040] Figure 2 Diagram of the steady-state delivery system of A4 and A5 glutathione composite freeze-dried efficacy core balls and B4 and B5 glutathione composite essence oil efficacy core balls, both samples are from Examples 4-5;

[0041] Figure 3 Diagrams of the steady-state delivery system of A6 and A7 anthocyanin-Vc composite freeze-dried efficacy core balls and B6 and B7 anthocyanin-Vc composite essence oil efficacy core balls. The samples are from Examples 6-7.

[0042] Figure 4 A diagram of the steady-state delivery system of A8, A9EUK134 composite freeze-dried efficacy core balls and a diagram of the steady-state delivery system of B8, B9EUK134 composite essence oil efficacy core balls, both samples are from Examples 8-9;

[0043] Figure 5 Diagrams of the steady-state delivery system of A10 and A11 blue copper peptide compound freeze-dried efficacy core balls and B10 and B11 blue copper peptide compound essential oil efficacy core balls, both samples are from Examples 10 and 11;

[0044] Figure 6It is an essence emulsion diagram of a compound essence oil efficacy core ball steady-state delivery system; wherein: C2, ergothioneine compound efficacy core ball steady-state delivery system essence emulsion II; C3, ergothioneine compound efficacy core ball steady-state delivery system essence emulsion III; C4, glutathione compound efficacy core ball steady-state delivery system essence emulsion IV; C5, glutathione compound efficacy core ball steady-state delivery system essence emulsion V; C6, anthocyanin-Vc compound efficacy core ball steady-state delivery system essence emulsion VI; C7, anthocyanin-Vc compound efficacy core ball steady-state delivery system essence emulsion VII; C8, EUK134 compound efficacy core ball steady-state delivery system essence emulsion VIII; C9, EUK134 compound efficacy core ball steady-state delivery system essence emulsion IX; C10, blue copper peptide compound efficacy core ball steady-state delivery system essence emulsion X; C11, blue copper peptide compound efficacy core ball steady-state delivery system essence emulsion XI;

[0045] Figure 7 The following are scanning electron micrographs of cross-sections of composite freeze-dried efficacy core ball steady-state delivery systems: A3, ergothioneine composite freeze-dried efficacy core ball steady-state delivery system; A5, glutathione composite freeze-dried efficacy core ball steady-state delivery system; A7, anthocyanidin-Vc composite freeze-dried efficacy core ball steady-state delivery system; A9, EUK134 composite freeze-dried efficacy core ball steady-state delivery system, samples from Examples 3, 5, 7, and 9, respectively;

[0046] Figure 8 This is a flow chart of the preparation process of the active ingredient complex efficacy core ball steady-state delivery system essence emulsion. DETAILED DESCRIPTION

[0047] Example 1 Preparation of Ergothioneine Compound Essential Oil Efficacy Core Ball Steady-State Delivery System No. 1

[0048] A. Weigh 0.3g thioneine, 0.6g recombinant type III collagen, 0.45g planool and 0.75g yeast beta glucan respectively, put into a beaker and mix;

[0049] B. Add 27.9g of purified water and heat and stir until the active ingredient is completely dissolved;

[0050] C. Use a peristaltic pump to add the above solution dropwise into a liquid nitrogen bucket. The droplet size is about 50 mg, and the solution is rapidly frozen to obtain small ice balls. During the dropwise addition, nitrogen gas needs to be introduced into the target solution to prevent the active ingredient from being oxidized and lost.

[0051] D. Transfer the small ice balls formed by freezing with liquid nitrogen to a freeze dryer and freeze-dry for 40 hours. The cold trap temperature of the freeze dryer is controlled at about -70°C (left and right refers to a deviation of 3°C, the same below), and the final temperature of the drying chamber is controlled at about 30°C. This will obtain the ergothioneine composite freeze-dried efficacy core ball steady-state delivery system, which is recorded as A1.

[0052] E. Separately weigh 39.5 g hydrogenated polyisobutene, 35 g coconut oil caprylate / caprate, 7.0 g fermented peony seed oil, 9.0 g isohexadecane, 5.0 g squalane, 3.0 g corn germ oil, 1.0 g acrylic acid (ester) copolymer, and 0.5 g Rosa damascena flower oil, place them in a beaker, and stir to mix thoroughly to obtain the essential oil;

[0053] F. Take a certain amount of freeze-dried functional core balls and place them in a container, such as a vial, glass bottle, or plastic bottle. Then add a certain amount of essential oil to obtain a composite essential oil functional core ball steady-state delivery system No. 1, denoted as B1.

[0054] Example 2 Preparation of Ergothioneine Compound Essential Oil Efficacy Core Ball Steady-State Delivery System II

[0055] A, weigh 0.3g thioneine, 0.48g recombinant type III collagen, 0.45g planoline, 0.54g yeast beta glucan, 0.3g trehalose and 0.06g ectoine respectively, put into beaker and mix;

[0056] B. Add 27.87g of purified water and heat with stirring until the active ingredient is completely dissolved;

[0057] C. Use a peristaltic pump to add the above solution dropwise into a liquid nitrogen bucket. The droplet size is about 50 mg, and the solution is rapidly frozen to obtain small ice balls. During the dropwise addition, nitrogen gas needs to be introduced into the target solution to prevent the active ingredient from being oxidized and lost.

[0058] D. The small ice balls formed by freezing with liquid nitrogen are transferred to a freeze dryer and freeze-dried for 40 hours. The cold trap temperature of the freeze dryer is controlled at about -70°C, and the final temperature of the drying chamber is controlled at about 30°C to obtain a steady-state delivery system of thioneine composite freeze-dried efficacy core balls, which is recorded as A2.

[0059] E. Separately weigh 39.5 g hydrogenated polyisobutene, 35 g coconut oil caprylate / caprate, 7.0 g fermented peony seed oil, 9.0 g isohexadecane, 5.0 g squalane, 3.0 g corn germ oil, 1.0 g acrylic acid (ester) copolymer, and 0.5 g Rosa damascena flower oil, place them in a beaker, and stir to mix thoroughly to obtain the essential oil;

[0060] F. Take a certain amount of freeze-dried functional core balls and place them in a container, such as a vial, glass bottle, or plastic bottle. Then add a certain amount of essential oil to obtain the composite essential oil functional core ball steady-state delivery system No. II, which is recorded as B2.

[0061] Example 3 Preparation of Ergothioneine Compound Essential Oil Efficacy Core Ball Steady-State Delivery System III

[0062] A, weigh 0.3g thioneine, 0.48g recombinant type III collagen, 0.45g planoan, 0.54g yeast beta glucan, 0.3g trehalose, 0.03g nonapeptide-1, 0.06g ectoine, 0.03g phloretin and 0.18g methyl beta cyclodextrin respectively, put into beaker and mix;

[0063] B. Add 27.66g of purified water and heat with stirring until the active ingredient is completely dissolved;

[0064] C. Use a peristaltic pump to add the above solution dropwise into a liquid nitrogen bucket. The droplet size is about 50 mg, and the solution is rapidly frozen to obtain small ice balls. During the dropwise addition, nitrogen gas needs to be introduced into the target solution to prevent the active ingredient from being oxidized and lost.

[0065] D. The small ice balls formed by freezing with liquid nitrogen are transferred to a freeze dryer and freeze-dried for 40 hours. The cold trap temperature of the freeze dryer is controlled at about -70°C, and the final temperature of the drying chamber is controlled at about 30°C to obtain a steady-state delivery system of thioneine composite freeze-dried efficacy core balls, which is recorded as A3.

[0066] E. Separately weigh 39.5 g hydrogenated polyisobutene, 35 g coconut oil caprylate / caprate, 7.0 g fermented peony seed oil, 9.0 g isohexadecane, 5.0 g squalane, 3.0 g corn germ oil, 1.0 g acrylic acid (ester) copolymer, and 0.5 g Rosa damascena flower oil, put them into a beaker, and stir to mix thoroughly to obtain the essential oil;

[0067] F. Take a certain amount of freeze-dried functional core balls and place them in a container, such as a vial, glass bottle, or plastic bottle. Then add a certain amount of essential oil to obtain the composite essential oil functional core ball steady-state delivery system No. III, which is recorded as B3.

[0068] Example 4 Preparation of Glutathione Composite Essential Oil Efficacy Core Ball Steady-State Delivery System IV

[0069] A. Weigh 0.24 g glutathione, 0.36 g recombinant type XVII collagen, 0.48 g yeast polysaccharide, 0.36 g sodium hyaluronate, 0.3 g madecassoside, 0.06 g acetyl hexapeptide-8, 0.12 g sodium DNA, and 0.18 g mannitol separately, place in a beaker and mix well.

[0070] B. Add 27.96 g of purified water and stir until the active ingredient is completely dissolved;

[0071] C. Use a peristaltic pump to add the above solution dropwise into a liquid nitrogen bucket. The droplet size is about 40 mg, and the solution is rapidly frozen to obtain small ice balls. During the dropwise addition, nitrogen gas needs to be introduced into the target solution to prevent oxidation and loss of the active ingredient.

[0072] D. The small ice balls formed by liquid nitrogen freezing are transferred to a freeze dryer and freeze-dried for 40 hours. The cold trap temperature of the freeze dryer is controlled at about -70°C, and the final temperature of the drying chamber is controlled at about 30°C to obtain a steady-state delivery system of thioneine composite freeze-dried efficacy core balls, which is recorded as A4.

[0073] E. Weigh 41 g of jojoba seed oil, 22 g of isododecane, 9 g of fermented sacha inchi oil, 8 g of fermented rice bran oil, 8 g of tocopheryl acetate, 11 g of sunflower seed oil, and 1 g of tea tree essential oil, respectively, put them into a beaker, and stir to mix well to obtain the essential oil;

[0074] F. Take a certain amount of freeze-dried functional core balls and place them in a container, such as a vial, glass bottle, plastic bottle, etc., and then add a certain amount of essential oil to obtain the composite essential oil functional core ball steady-state delivery system No. IV, recorded as B4.

[0075] Example 5 Preparation of Glutathione Composite Essential Oil Efficacy Core Ball Steady-State Delivery System No. V

[0076] A. Weigh 0.24 g glutathione, 0.36 g recombinant type XVII collagen, 0.48 g yeast polysaccharide, 0.36 g sodium hyaluronate, 0.3 g madecassoside, 0.01 g conopeptide, 0.12 g sodium DNA, 0.18 g mannitol, 0.06 g menthol, and 0.15 g hydroxypropyl β-cyclodextrin, respectively, and place in a beaker and mix thoroughly.

[0077] B. Add 27.75g of purified water and stir until the active ingredient is completely dissolved;

[0078] C. Use a peristaltic pump to add the above solution dropwise into a liquid nitrogen bucket. The droplet size is about 40 mg, and the solution is rapidly frozen to obtain small ice balls. During the dropwise addition, nitrogen gas needs to be introduced into the target solution to prevent oxidation and loss of the active ingredient.

[0079] D. The small ice balls formed by freezing with liquid nitrogen are transferred to a freeze dryer and freeze-dried for 40 hours. The cold trap temperature of the freeze dryer is controlled at about -70°C, and the final temperature of the drying chamber is controlled at about 30°C to obtain a steady-state delivery system of thioneine composite freeze-dried efficacy core balls, which is recorded as A5.

[0080] E. Weigh 41 g of jojoba seed oil, 22 g of isododecane, 9 g of fermented sacha inchi oil, 8 g of fermented rice bran oil, 8 g of tocopheryl acetate, 11 g of sunflower seed oil, and 1 g of tea tree essential oil, respectively, put them into a beaker, and stir to mix well to obtain the essential oil;

[0081] F. Take a certain amount of freeze-dried functional core balls and place them in a container, such as a vial, glass bottle, plastic bottle, etc. Then add a certain amount of essential oil to obtain compound essential oil functional core balls No. V, recorded as B5.

[0082] Example 6 Preparation of Anthocyanin-Vc Composite Essence Oil Efficacy Core Ball Steady-State Delivery System No. VI

[0083] A. Weigh 0.09g anthocyanidin, 0.54g recombinant fibronectin, 0.15g xanthan gum, 0.45g polyglutamic acid, 0.3g asiaticoside, 0.42g vitamin C (Vc), 0.15g Vc glucoside, and 0.3g theanine separately, put them into a beaker and mix well.

[0084] B. Add 27.75g of purified water and stir until the active ingredient is completely dissolved;

[0085] C. Use a peristaltic pump to add the above solution dropwise into a liquid nitrogen bucket. The droplet size is about 40 mg. Quickly freeze to obtain small ice balls. During the dropwise addition, nitrogen gas should be introduced into the target solution to prevent oxidation and loss of the active ingredient.

[0086] D. The small ice balls formed by freezing with liquid nitrogen are transferred to a freeze dryer and freeze-dried for 40 hours. The cold trap temperature of the freeze dryer is controlled at about -70°C, and the final temperature of the drying chamber is controlled at about 30°C to obtain a steady-state delivery system of thioneine composite freeze-dried efficacy core balls, which is recorded as A6.

[0087] E. Weigh 33 g of fermented grape seed oil, 32 g of ethylhexyl palmitate, 8 g of S. sutchuenensis seed fat, 9 g of rapeseed oil, 12 g of macadamia seed oil, 5 g of squalane, and 1.0 g of essence respectively, put them into a beaker, and stir to mix well to obtain the essential oil;

[0088] F. Take a certain amount of freeze-dried functional core balls and place them in a container, such as a vial, glass bottle, plastic bottle, etc., and then add a certain amount of essential oil to obtain the composite essential oil functional core ball steady-state delivery system No. VI, recorded as B6.

[0089] Example 7 Preparation of Anthocyanin-Vc Composite Essence Oil Efficacy Core Ball Steady-State Delivery System No. VII

[0090] A. Weigh 0.09g anthocyanidin, 0.54g recombinant fibronectin, 0.15g xanthan gum, 0.45g polyglutamic acid, 0.3g asiaticoside, 0.42g vitamin C, 0.15g Vc glucoside, 0.3g theanine, 0.03g butylene glycol, and 0.03g phloretin respectively, put them into a beaker and mix well;

[0091] B. Add 27.69 g of purified water and stir until the active ingredient is completely dissolved;

[0092] C. Use a peristaltic pump to add the above solution dropwise into a liquid nitrogen bucket. The droplet size is about 40 mg, and the solution is rapidly frozen to obtain small ice balls. During the dropwise addition, nitrogen gas needs to be introduced into the target solution to prevent oxidation and loss of the active ingredient.

[0093] D. The small ice balls formed by freezing with liquid nitrogen are transferred to a freeze dryer and freeze-dried for 40 hours. The cold trap temperature of the freeze dryer is controlled at about -70°C, and the final temperature of the drying chamber is controlled at about 30°C to obtain a steady-state delivery system of thioneine composite freeze-dried efficacy core balls, which is recorded as A7.

[0094] E. Weigh 33 g of fermented grape seed oil, 32 g of ethylhexyl palmitate, 8 g of S. sutchuenensis seed fat, 9 g of rapeseed oil, 12 g of macadamia seed oil, 5 g of squalane, and 1.0 g of essence respectively, put them into a beaker, and stir to mix well to obtain the essential oil;

[0095] F. Take a certain amount of freeze-dried functional core balls and place them in a container, such as a vial, glass bottle, plastic bottle, etc., and then add a certain amount of essential oil to obtain the composite essential oil functional core ball steady-state delivery system No. VII, recorded as B7.

[0096] Example 8 Preparation of EUK134 Composite Essence Oil Efficacy Core Ball Steady-State Delivery System No. VIII

[0097] A. Weigh 0.09g EUK134, 0.48g yeast protein, 0.3g recombinant collagen, 0.24g gellan gum, 0.18g fermented Ganoderma lucidum polysaccharide, 0.45g swiftlet acid, 0.09g snake venom peptide, and 0.12g arbutin respectively, put them into a beaker and mix well.

[0098] B. Add 28.14 g of purified water and stir until the active ingredient is completely dissolved;

[0099] C. Use a peristaltic pump to add the above solution dropwise into a liquid nitrogen bucket. The droplet size is about 40 mg, and the solution is rapidly frozen to obtain small ice balls. During the dropwise addition, nitrogen gas needs to be introduced into the target solution to prevent oxidation and loss of the active ingredient.

[0100] D. The small ice balls formed by liquid nitrogen freezing are transferred to a freeze dryer and freeze-dried for 40 hours. The cold trap temperature of the freeze dryer is controlled at about -70°C, and the final temperature of the drying chamber is controlled at about 30°C to obtain a steady-state delivery system of thioneine composite freeze-dried efficacy core balls, which is recorded as A8.

[0101] E. Separately weigh 22 g of isononyl isononanoate, 37 g of caprylic / capric triglyceride, 5 g of Sphaerocarpus serrata seed butter, 20 g of meadowfoam seed oil, 5 g of dimethicone, 10 g of fermented grape seed oil, and 1.0 g of lavender essential oil, put them into a beaker, and then stir to mix evenly to obtain the essential oil;

[0102] F. Take a certain amount of freeze-dried functional core balls and place them in a container, such as a vial, glass bottle, or plastic bottle. Then add a certain amount of essential oil to obtain the composite essential oil functional core ball steady-state delivery system No. VIII, which is designated as B8.

[0103] Example 9 Preparation of EUK134 Composite Essence Oil Efficacy Core Ball Steady-State Delivery System No. IX

[0104] A. Weigh 0.09g EUK134, 0.48g yeast protein, 0.3g recombinant collagen, 0.24g gellan gum, 0.18g fermented Ganoderma lucidum polysaccharide, 0.45g swiftlet acid, 0.12g arbutin, 0.03g phloretin, 0.18g hydroxypropyl β-cyclodextrin, and 0.03g butylene glycol separately, place in a beaker and mix well.

[0105] B. Add 27.9 g of purified water and stir until the active ingredient is completely dissolved;

[0106] C. Use a peristaltic pump to add the above solution dropwise into a liquid nitrogen bucket. The droplet size is about 40 mg, and the solution is rapidly frozen to obtain small ice balls. During the dropwise addition, nitrogen gas needs to be introduced into the target solution to prevent oxidation and loss of the active ingredient.

[0107] D. The small ice balls formed by freezing with liquid nitrogen are transferred to a freeze dryer and freeze-dried for 40 hours. The cold trap temperature of the freeze dryer is controlled at about -70°C, and the final temperature of the drying chamber is controlled at about 30°C to obtain a steady-state delivery system of thioneine composite freeze-dried efficacy core balls, which is recorded as A9.

[0108] E. Separately weigh 22 g of isononyl isononanoate, 37 g of caprylic / capric triglyceride, 5 g of Sphaerocarpus serrata seed butter, 20 g of meadowfoam seed oil, 5 g of dimethicone, 10 g of fermented grape seed oil, and 1.0 g of lavender essential oil, put them into a beaker, and then stir and mix them evenly to obtain the essential oil;

[0109] F. Take a certain amount of freeze-dried functional core balls and place them in a container, such as a vial, glass bottle, plastic bottle, etc., and then add a certain amount of essential oil to obtain the composite essential oil functional core ball steady-state delivery system No. IX, recorded as B9.

[0110] Example 10 Preparation of Blue Copper Peptide Composite Essential Oil Efficacy Core Ball Steady-State Delivery System No. X

[0111] A. Weigh 0.24g of blue copper peptide, 0.36g of recombinant type III collagen, 0.6g of yeast beta-glucan, 0.45g of sodium hyaluronate, 0.18g of niacinamide, 0.09g of ectoine, 0.09g of Vc ethyl ether, 0.45g of hydroxypropyl beta-cyclodextrin, and 0.06g of phloretin respectively, put them into a beaker and mix well;

[0112] B. Add 27.48 g of purified water and stir until the active ingredient is completely dissolved;

[0113] C. Use a peristaltic pump to add the above solution dropwise into a liquid nitrogen bucket. The droplet size is about 40 mg, and the solution is rapidly frozen to obtain small ice balls. During the dropwise addition, nitrogen gas needs to be introduced into the target solution to prevent oxidation and loss of the active ingredient.

[0114] D. The small ice balls formed by freezing with liquid nitrogen are transferred to a freeze dryer and freeze-dried for 40 hours. The cold trap temperature of the freeze dryer is controlled at about -70°C, and the final temperature of the drying chamber is controlled at about 30°C to obtain a steady-state delivery system of thioneine composite freeze-dried efficacy core balls, which is recorded as A10.

[0115] E. Separately weigh 22 g of isononyl isononanoate, 37 g of caprylic / capric triglyceride, 5 g of Sphaerocarpus serrata seed butter, 20 g of meadowfoam seed oil, 5 g of dimethicone, 10 g of fermented grape seed oil, and 1.0 g of lavender essential oil, put them into a beaker, and then stir and mix them evenly to obtain the essential oil;

[0116] F. Take a certain amount of freeze-dried functional core balls and place them in a container, such as a vial, glass bottle, or plastic bottle. Then add a certain amount of essential oil to obtain a composite essential oil functional core ball steady-state delivery system No. X, denoted as B10.

[0117] Example 11 Preparation of Blue Copper Peptide Composite Essential Oil Efficacy Core Ball Steady-State Delivery System No. XI

[0118] A. Weigh 0.15g of blue copper peptide, 0.06g of nicotinamide, 0.3g of recombinant fibronectin, 0.36g of pullulan, 0.3g of sodium hyaluronate, 0.27g of theanine, 0.15g of trehalose, and 0.03g of butanediol respectively, place them in a beaker and mix well.

[0119] B. Add 27.69g of purified water and stir until the active ingredient is completely dissolved;

[0120] C. Use a peristaltic pump to add the above solution dropwise into a liquid nitrogen bucket. The droplet size is about 40 mg, and the solution is rapidly frozen to obtain small ice balls. During the dropwise addition, nitrogen gas needs to be introduced into the target solution to prevent oxidation and loss of the active ingredient.

[0121] D. Transfer the small ice balls formed by freezing with liquid nitrogen to a freeze dryer and freeze-dry for 40 hours. The cold trap temperature of the freeze dryer is controlled at about -70°C, and the final temperature of the drying chamber is controlled at about 30°C. This will yield the thioneine composite freeze-dried functional core balls, designated as A11.

[0122] E. Separately weigh 22 g of isononyl isononanoate, 37 g of caprylic / capric triglyceride, 5 g of Sphaerocarpus serrata seed butter, 20 g of meadowfoam seed oil, 5 g of dimethicone, 10 g of fermented grape seed oil, and 1.0 g of lavender essential oil, put them into a beaker, and then stir and mix them evenly to obtain the essential oil;

[0123] F. Take a certain amount of freeze-dried functional core balls and place them in a container, such as a vial, glass bottle, or plastic bottle. Then add a certain amount of essential oil to obtain a composite essential oil functional core ball steady-state delivery system No. XI, recorded as B11.

[0124] Test Example 1: Appearance of the composite freeze-dried functional core ball steady-state delivery system and the composite essential oil functional core ball steady-state delivery system

[0125] Use a camera to take pictures to characterize the appearance and morphology of the composite freeze-dried efficacy core ball steady-state delivery system and the composite essence oil efficacy core ball steady-state delivery system, and conduct comparative analysis.

[0126] 1) Ergothioneine compound essential oil efficacy core ball steady-state delivery system series: Figure 1 It can be seen from A1, A2 and A3 in that after freeze drying, embodiment 1-3 can all obtain white thioneine composite freeze-dried efficacy core ball steady-state delivery system, i.e., beautiful core ball, and most of the pellets are all good spherical, and the surface is relatively smooth. There is a little crackle on the pellet surface, and this is mainly due to uneven internal stress during freezing, which does not affect subsequent use. From the sample appearance, 3 samples do not have obvious differences. After adding essence oil, essence oil fills up the hole of freeze-dried core ball rapidly, obtains compound essence oil efficacy core ball steady-state delivery system B1, B2 and B3, and perfect spherical structure outward appearance remains intact, and the structural stability of efficacy core ball and the shelf life stability of active substance have also been significantly improved.

[0127] 2) Glutathione complex essence oil efficacy core ball steady-state delivery system series: Figure 2 A4 and A5 are glutathione composite freeze-dried efficacy core ball steady-state delivery systems from Examples 4 and 5. In samples A4 and A5, the white spheres are mostly well-spherical and have relatively smooth surfaces. There are a few cracks on the surface of the spheres, which are mainly caused by uneven internal stress during freezing and do not affect subsequent use. After adding the essence oil, the composite essence oil efficacy core balls B4 and B5 are obtained. The perfect spherical structure and appearance remain intact, and the structural stability of the efficacy core balls and the shelf life stability of the active ingredients are also significantly improved.

[0128] 3) Anthocyanin-Vc compound essence oil stable delivery system: Figure 3 This is a stable delivery system of anthocyanin-Vc composite freeze-dried efficacy core balls from Examples 6 and 7. In samples A6 and A7, the vast majority of the rose-colored balls have a good spherical shape and a relatively smooth surface. There are a few cracks on the surface of the balls, which is mainly caused by uneven internal stress during freezing and does not affect subsequent use. After adding the essence oil, the composite essence oil efficacy core balls B6 and B7 are obtained. The color of the core balls becomes brighter, the perfect spherical structure appearance remains intact, and the structural stability of the efficacy core balls and the shelf life stability of the active ingredients are also significantly improved.

[0129] 4) EUK134 compound essential oil efficacy core ball steady-state delivery system: Figure 4 This is the EUK134 composite freeze-dried efficacy core ball steady-state delivery system from Examples 8 and 9. In samples A8 and A9, the vast majority of the yellow balls have a good spherical shape and a relatively smooth surface. There are a few cracks on the surface of the balls, which is mainly caused by uneven internal stress during freezing and does not affect subsequent use. After adding the essence oil, the composite essence oil efficacy core balls B8 and B9 are obtained. The color of the core ball becomes brighter, the perfect spherical structure appearance remains intact, and the structural stability of the efficacy core ball and the shelf life stability of the active ingredient are also significantly improved.

[0130] 5) Blue Copper Peptide Complex Essence Oil Efficacy Core Ball Steady-State Delivery System: Figure 5 It is a blue copper peptide composite freeze-dried efficacy core ball steady-state delivery system from Examples 10 and 11. In samples A10 and A11, most of the blue balls have a good spherical shape and a relatively smooth surface. There are a few cracks on the surface of the balls, which is mainly caused by uneven internal stress during freezing and does not affect subsequent use. After adding the essence oil, the composite essence oil efficacy core ball steady-state delivery systems B10 and B11 are obtained. The core ball color becomes brighter, the perfect spherical structure appearance remains intact, and the structural stability of the efficacy core ball and the shelf life stability of the active substance are also significantly improved.

[0131] Test Example 2: Appearance of Essence Emulsion with Compound Essence Oil Efficacy Core Ball Steady-State Delivery System

[0132] Based on the compound essence oil core ball, a fresh essence lotion can be prepared, namely the compound efficacy core ball steady-state delivery system essence lotion, see the application case for details. Use a camera to take pictures, characterize the compound efficacy core ball essence lotion, and conduct comparative analysis ( Figure 6 ).

[0133] from Figure 6As can be seen, C2, C3, C4, C5, C6, and C7 are milk-cap-like essence emulsions. This is primarily due to the inclusion of emulsifiers, such as Tween 20, Laureth-4, and PEG-40 hydrogenated castor oil, in the vehicle. When the essence emulsion is shaken to prepare, the stable delivery system of the functional core ball dissolves in the vehicle, while the essential oil is emulsified into tiny droplets. After a period of time, the droplets float to the surface, resulting in a milk-cap-like essence emulsion. In contrast, C8, C9, C10, and C11 are double-layered, transparent essences, primarily due to the absence of an emulsifier in the vehicle. Although the essential oil forms tiny droplets when shaken to prepare the essence emulsion, the lack of an emulsifier prevents a stable emulsion from forming. After a period of time, the oil and water phases separate, resulting in a double-layered, transparent essence. This double-layered, transparent essence does not affect consumer use; instead, it helps form a protective layer on the skin when applied.

[0134] Detection Example 3 Scanning Electron Microscope Image

[0135] Figure 7 The following are scanning electron microscope images of samples A3, A5, A7, and A9. The stable delivery system of the functional core ball was gently broken open and then observed using a scanning electron microscope. As can be seen from the figure, the interior of the ball is a uniform layered structure, with evenly distributed micron-scale channels between the layers. During rapid freezing with liquid nitrogen, the active substances dissolved in the water quickly precipitate to form extremely small crystals. Then, during freeze-drying, when the ice crystals sublime, water vapor forms micron-scale water vapor channels, connecting the tiny active substance crystals into one piece to form a layered structure. Because liquid nitrogen freezes very quickly, the microstructure of the entire ball is very uniform. The active substance layer is very thin, basically less than 1 micron, which ensures that the active substance can be dissolved extremely quickly when water is added again.

[0136] Test Example 4: Stability

[0137] Active ingredients such as ergothioneine, glutathione, anthocyanins, blue copper peptides, vitamin C and EUK134 have poor stability, especially in aqueous formulation systems. They are easily oxidized, complexed or decomposed, resulting in loss of biological activity.

[0138] Using the same principles as cosmetic stability evaluation, the stability of the aforementioned freeze-dried and essential oil-based compound spheres and spheres was evaluated through accelerated testing at 45°C and compared to a control serum. Accelerated testing was performed on the freeze-dried and essential oil-based compound spheres, followed by analysis of solutions prepared at varying concentrations. The retained concentration of the target active ingredient as a percentage of the initial concentration was measured at various aging times, with the initial concentration of the target active ingredient set as 100%. The results are shown in the table below.

[0139] A. Ergothioneine compound efficacy core ball steady-state delivery system series samples

[0140] As shown in Table 1, after 28 days of accelerated testing, the ergothioneine content or concentration in the ergothioneine essence solution decreased by approximately 10%. The change in ergothioneine content in the three ergothioneine composite freeze-dried functional pellets was very small, with a decrease of less than 1% (approximately 0.8%). This indicates that the stability of ergothioneine in the composite freeze-dried functional pellets is greatly improved. In contrast, the ergothioneine content in the steady-state delivery system of the ergothioneine composite essential oil functional pellets remained virtually unchanged, decreasing by approximately 0.1%, which is approximately one-eighth the decrease in the freeze-dried functional pellets. This suggests that the addition of the essential oil to the freeze-dried functional pellets further blocks the effects of oxygen and even light on ergothioneine, further improving its stability.

[0141] Table 1. Stability of Ergothioneine Complex Functional Core Ball Steady-State Delivery System Series Products

[0142]

[0143] B. Glutathione compound efficacy core ball steady-state delivery system series samples

[0144] As shown in Table 2, glutathione stability is relatively poor. After a one-day accelerated experiment, approximately 99% of the glutathione in the control serum had been oxidatively degraded, leaving only about 0.3% of its original content. After seven days, it was completely undetectable. The glutathione content in both glutathione-composite freeze-dried functional pellets showed very little change, decreasing by less than 2% (approximately 1.9%) after 28 days. This indicates that glutathione stability is significantly improved in the freeze-dried functional pellets. Furthermore, the glutathione content in the glutathione-composite essential oil functional pellets stable delivery system showed even less change, decreasing by approximately 0.9%, less than half the decrease in the freeze-dried functional pellets. This suggests that the addition of essential oil to the freeze-dried functional pellets stable delivery system further blocks the effects of oxygen and even light on glutathione, further improving its stability.

[0145] Table 2. Stability of glutathione compound functional core ball series products

[0146]

[0147] C. Anthocyanin-Vc composite functional core ball steady-state delivery system series samples

[0148] As shown in Table 3, anthocyanin stability is relatively poor. After a one-day accelerated test, approximately 99% of the glutathione in the control serum had been oxidatively degraded, leaving the content at approximately 1% of its original level. After 7 days, it was completely undetectable. The anthocyanin content in both anthocyanin-Vc composite freeze-dried functional pellets showed very little change, decreasing by less than 2% (approximately 1.7%) after 28 days. This indicates that the stability of anthocyanins in the composite freeze-dried functional pellets is significantly improved. In the anthocyanin-Vc composite essential oil functional pellets stable delivery system, the anthocyanin content changed even more, decreasing by approximately 0.7%, less than half the decrease seen in the freeze-dried functional pellets. This suggests that the addition of essential oil to the freeze-dried functional pellets further blocks the effects of oxygen and even light on anthocyanin content, further improving its stability.

[0149] Table 3. Anthocyanin stability in the anthocyanin-Vc composite efficacy core ball steady-state delivery system series

[0150]

[0151] Similarly, Table 4 shows that after a one-day accelerated experiment, over 99% of the vitamin C in the control serum had been oxidatively degraded, leaving only approximately 1% of its original content. After 7 days, it was completely undetectable. The vitamin C content in both anthocyanin-Vc composite freeze-dried functional pellets exhibited very little change, decreasing by less than 2% (approximately 1.9%) after 28 days. This indicates that the stability of vitamin C in the composite freeze-dried functional pellets is significantly improved. Furthermore, the vitamin C content in the anthocyanin-Vc composite essential oil functional pellets exhibited even less change, decreasing by approximately 0.65%, which is approximately one-third of the decrease observed in the freeze-dried functional pellets. This suggests that the addition of essential oil to the freeze-dried functional pellets further blocks the effects of oxygen and even light on vitamin C, further enhancing its stability.

[0152] Table 4. Stability of Vitamin C in the Anthocyanin-Vc Composite Efficacy Core Ball Steady-State Delivery System Series

[0153]

[0154] D. EUK134 composite functional core ball steady-state delivery system series samples

[0155] As can be seen from Table 5, after 28 days of accelerated testing, the EUK134 content or concentration in the EUK134 essence decreased by about 13%. The change in EUK134 in the two EUK134 composite freeze-dried efficacy core ball steady-state delivery systems was very small, decreasing by about 1%. This shows that in the composite freeze-dried efficacy core ball steady-state delivery system, the stability of EUK134 is greatly improved. In the EUK134 composite essential oil efficacy core ball steady-state delivery system, the EUK134 content remained almost unchanged, decreasing by about 0.3%, which is about one-third of the decrease in the freeze-dried efficacy core ball steady-state delivery system. This shows that by adding essential oil to the freeze-dried efficacy core ball steady-state delivery system, the essential oil can further block the effects of oxygen and even light on EUK134, further improving its storage stability.

[0156] Table 5. Stability of EUK134 composite functional core ball steady-state delivery system products

[0157]

[0158] F. Blue copper peptide compound efficacy core ball steady-state delivery system series samples

[0159] As shown in Table 6, after 28 days of accelerated testing, the copper peptide content or concentration in the copper peptide serum decreased by approximately 17%. The change in copper peptide content in both copper peptide-combined freeze-dried functional pellets was minimal, decreasing by approximately 0.9%. This indicates that the copper peptide stability was significantly improved in the freeze-dried functional pellets. In contrast, the EUK134 content in the EUK134-combined essential oil functional pellets remained virtually unchanged, decreasing by approximately 0.2%, roughly one-quarter the decrease observed in the freeze-dried functional pellets. This suggests that the addition of essential oil to the freeze-dried functional pellets further blocks the effects of oxygen and light on the copper peptide, further improving its storage stability.

[0160] Table 6. Stability of the Blue Copper Peptide Complex Efficacy Core Ball Steady-State Delivery System Series

[0161]

[0162] Test Example 5: Transdermal Absorption

[0163] For each application case of the steady-state delivery system of the compound essence oil with different active ingredients, corresponding control essences were selected and systematic transdermal efficacy evaluation and comparison were conducted. The evaluation method was based on the "In Vitro Test Method for Skin Absorption of Chemicals". The evaluation results are as follows:

[0164] Table 7. Transdermal evaluation data of active ingredient compound essence oil efficacy core ball and its control essence solution

[0165]

[0166]

[0167]

[0168] For thioneine series application examples 1-3, as can be seen from Table 7, thioneine composite efficacy core ball steady-state delivery system essence emulsion II transdermal effect, is significantly better than thioneine control essence, and 24h transdermal amount is about nearly 3 times of control essence.And thioneine composite efficacy core ball steady-state delivery system essence emulsion III added with penetration enhancer, transdermal effect is better, and 24h transdermal amount is about more than 4 times of control essence.

[0169] For glutathione application examples 4-6, only 1-hour and 4-hour transdermal effects were tested because glutathione is very unstable and easily oxidized and deteriorated. As can be seen in Table 7, the transdermal effect of glutathione composite core ball steady-state delivery system essence emulsion IV was significantly better than that of the glutathione control essence, with a 4-hour transdermal volume approximately twice that of the control essence. Glutathione composite core ball steady-state delivery system essence emulsion V, which added a penetration enhancer, showed even better transdermal effect, with a 4-hour transdermal volume approximately three times that of the control essence.

[0170] For anthocyanin-Vc series application examples 7-9, due to the poor stability of anthocyanin and Vc, only the 1h and 4h transdermal effects were tested. As can be seen from Table 7, the transdermal effect of anthocyanin-Vc composite core ball steady-state delivery system essence lotion VI is significantly better than the anthocyanin-Vc control essence. The 4h transdermal amount of anthocyanin is about 2.5 times that of the control essence, and the 4h transdermal amount of Vc is about 2 times that of the control essence. The composite functional core ball steady-state delivery system essence lotion VII, which has a penetration enhancer, has an even better transdermal effect. The 4h transdermal amount of anthocyanin is about 4 times that of the control essence, and the 4h transdermal amount of Vc is about 3 times that of the control essence.

[0171] For EUK134 series application examples 10-12, as shown in Table 7, the transdermal performance of EUK134 Complex Functional Core-Sphere Steady-State Delivery System Essence Emulsion VIII was significantly better than that of the EUK134 control essence, with a 24-hour transdermal volume nearly double that of the control essence. EUK134 Complex Functional Core-Sphere Steady-State Delivery System Essence Emulsion IX, which added a penetration enhancer, achieved even better transdermal performance, with a 24-hour transdermal volume approximately three times that of the control essence.

[0172] For the Copper Peptide Series Application Examples 13-15, both Copper Peptide Complex Functional Core-Ball Steady-State Delivery Systems incorporate a penetration enhancer active ingredient. As shown in Table 7, the transdermal efficacy of Copper Peptide Complex Functional Core-Ball Steady-State Delivery System Essence Emulsions X and XI significantly outperformed the Copper Peptide Control Essence, with 24-hour transdermal penetration rates approximately four times that of the Control Essence.

[0173] Application Example 1 Ergothioneine Control Essence

[0174] (1) Weigh 86.6 g of purified water, 5.0 g of butylene glycol, 0.80 g of Chondrus crispus, and 0.40 g of raspberry ketone in sequence, place them in a container, and heat at 40°C with stirring until they are evenly dissolved;

[0175] (2) After stirring and cooling, add 5.0g of Rosa damascena flower water, 2.0g of 1,2-pentanediol and 0.20g of ergothioneine in sequence, stir evenly, and then discharge the material to obtain the ergothioneine control essence solution for subsequent evaluation.

[0176] Application Example 2 Ergothioneine Complex Efficacy Core Ball Steady-state Delivery System Essence Emulsion II

[0177] (1) Weigh 1.0 g of ergothioneine compound essential oil functional core ball steady-state delivery system B2 (including 0.95 g of essential oil and 0.05 g of A2 functional core ball) and place it into a vial;

[0178] (2) Meixin ball solvent configuration:

[0179] 91.5 g of purified water, 2.0 g of butylene glycol, 0.5 g of Tremella polysaccharide, 0.2 g of glycerol glucoside, 0.2 g of Chondrus crispus, 0.5 g of PEG-40 hydrogenated castor oil, 1.0 g of Tween-20, 0.5 g of p-hydroxyacetophenone, 1.0 g of 1,2-hexanediol, 0.5 g of 1,2-pentanediol, and 2.1 g of Rosa damascena flower water were weighed in sequence and placed in a container; heated at 40° C. and stirred to dissolve evenly, and after stirring and cooling, the material was discharged to obtain a Meixin ball solvent solution.

[0180] (3) Use a pipette to add 3 mL of the Meixinqiu solvent solution into the vial containing 1.0 g of the compound essential oil efficacy core ball steady-state delivery system B2;

[0181] (4) Gently shake the compound freeze-dried functional core ball steady-state delivery system to dissolve in the solvent liquid, and the solvent liquid emulsifies the essential oil to obtain an essence emulsion. After standing for 5 minutes, the essence emulsion partially floats up to obtain the milk-cap-type ergothioneine compound functional core ball steady-state delivery system essence emulsion II (see Figure 6 Essence lotion, C2); for subsequent evaluation.

[0182] Application Example 3 Ergothioneine Complex Efficacy Core Ball Steady-state Delivery System Essence Emulsion III

[0183] (1) Weigh 1.0 g of ergothioneine compound essential oil functional core ball steady-state delivery system B3 (including 0.95 g of essential oil and 0.05 g of A3 functional core ball) and place it into a vial;

[0184] (2) Meixin ball solvent configuration:

[0185] 91.5 g of purified water, 2.0 g of butylene glycol, 0.5 g of Tremella polysaccharide, 0.2 g of glycerol glucoside, 0.2 g of Chondrus crispus, 0.5 g of PEG-40 hydrogenated castor oil, 1.0 g of Tween-20, 0.5 g of p-hydroxyacetophenone, 1.0 g of 1,2-hexanediol, 0.5 g of 1,2-pentanediol, and 2.1 g of Rosa damascena flower water were weighed in sequence and placed in a container; heated at 40° C. and stirred to dissolve evenly, and after stirring and cooling, the material was discharged to obtain a Meixin ball solvent solution.

[0186] (3) Use a pipette to add 3 mL of the Meixinqiu solvent solution into the vial containing 1.0 g of the compound essential oil efficacy core ball steady-state delivery system B3;

[0187] (4) Gently shake the compound freeze-dried functional core ball steady-state delivery system to dissolve in the solvent liquid, and the solvent liquid emulsifies the essential oil to obtain an essence emulsion. After standing for 5 minutes, the essence emulsion partially floats up to obtain the milk-cap-type ergothioneine compound functional core ball steady-state delivery system essence emulsion III (see Figure 6 Essence lotion, C3); for subsequent evaluation.

[0188] Application Example 4 Glutathione Control Essence

[0189] (1) Weigh 86.6 g of purified water, 5.0 g of butylene glycol, 0.80 g of Chondrus crispus, and 0.40 g of raspberry ketone in sequence, place them in a container, and heat at 40°C with stirring until they are evenly dissolved;

[0190] (2) After stirring and cooling, add 5.0g of Rosa damascena floral water, 2.0g of 1,2-pentanediol and 0.20g of glutathione in sequence and stir for about 10 minutes to obtain a uniform solution. Then, the material can be discharged to obtain a glutathione control essence for subsequent evaluation.

[0191] Application Example 5 Glutathione Complex Efficacy Core Ball Steady-state Delivery System Essence Emulsion IV

[0192] (1) Weigh 1.0 g of glutathione complex essential oil functional core ball steady-state delivery system B4 (including 0.95 g of essential oil and 0.05 g of A4 functional core ball) and place it into a vial;

[0193] (2) Meixin ball solvent configuration:

[0194] Weigh 94.5 g of purified water, 1.8 g of butylene glycol, 0.5 g of wild soybean seed extract, 0.5 g of cocoyl glucoside, 0.8 g of PEG stearate, 0.3 g of hydroxyethyl cellulose, 0.1 g of hydrogenated starch hydrolysate, 0.5 g of p-hydroxyacetophenone, and 1.0 g of 1,2-pentanediol in sequence and put them into a container; heat and stir at 40°C to dissolve evenly, stir and cool, then discharge the material to obtain the Meixinqiu solvent solution.

[0195] (3) Use a pipette to add 3 mL of the Meixinqiu solvent solution into the vial containing 1.0 g of the compound essential oil efficacy core ball steady-state delivery system B4;

[0196] (4) Gently shake the compound freeze-dried functional core ball steady-state delivery system to dissolve in the solvent liquid, and the solvent liquid will emulsify the essential oil to obtain the essence emulsion. After standing for 5 minutes, the essence emulsion will partially float up to obtain the milk cap type glutathione compound functional core ball essence emulsion IV (see Figure 6 Essence lotion, C4); for subsequent evaluation.

[0197] Application Example 6 Glutathione Complex Efficacy Core Ball Steady-state Delivery System Essence Lotion V

[0198] (1) Weigh 1.0 g of glutathione complex essential oil functional core ball steady-state delivery system B5 (including 0.95 g of essential oil and 0.05 g of A5 functional core ball) and place it into a vial;

[0199] (2) Meixin ball solvent configuration:

[0200] Weigh 94.5 g of purified water, 1.8 g of butylene glycol, 0.5 g of wild soybean seed extract, 0.5 g of cocoyl glucoside, 0.8 g of PEG stearate, 0.3 g of hydroxyethyl cellulose, 0.1 g of hydrogenated starch hydrolysate, 0.5 g of p-hydroxyacetophenone, and 1.0 g of 1,2-pentanediol in sequence and put them into a container; heat and stir at 40°C to dissolve evenly, stir and cool, then discharge the material to obtain the Meixinqiu solvent solution.

[0201] (3) Use a pipette to add 3 mL of the Meixinqiu solvent solution into the vial containing 1.0 g of the compound essential oil efficacy core ball steady-state delivery system B5;

[0202] (4) Gently shake the compound freeze-dried functional core ball steady-state delivery system to dissolve in the solvent liquid, and the solvent liquid emulsifies the essential oil to obtain the essence emulsion. After standing for 5 minutes, the essence emulsion partially floats up to obtain the milk cap type glutathione compound functional core ball steady-state delivery system essence emulsion V (see Figure 6 Essence lotion, C5); for subsequent evaluation.

[0203] Application Example 7 Anthocyanin-Vc Control Essence

[0204] (1) Weigh 86.4 g of purified water, 5.0 g of butylene glycol, 0.80 g of Chondrus crispus, and 0.40 g of raspberry ketone in sequence, place them in a container, heat at 40°C, and stir until dissolved evenly;

[0205] (2) After stirring and cooling, add 5.0 g of Rosa damascena flower water, 2.0 g of 1,2-pentanediol, 0.10 g of anthocyanin and 0.30 g of vitamin C in sequence and stir for about 10 minutes to obtain a uniform solution. The material can be discharged for subsequent evaluation.

[0206] Application Example 8 Anthocyanin-Vc Composite Efficacy Core Ball Steady-State Delivery System Essence Emulsion VI

[0207] (1) Weigh 1.0 g of anthocyanin-Vc composite essential oil functional core ball steady-state delivery system B6 (including 0.95 g of essential oil and 0.05 g of A6 functional core ball) and place it into a vial;

[0208] (2) Meixin ball solvent configuration:

[0209] 93.0 g of purified water, 1.5 g of Tween 80, 0.7 g of laureth-4, 0.5 g of xylitol glucoside, 0.6 g of anhydroxylitol, 0.5 g of sodium hyaluronate, 1.6 g of polyglycerol-10, 0.8 g of p-hydroxyacetophenone, and 0.8 g of hexylene glycol were weighed in sequence and placed in a container; heated and stirred at 40° C. until uniformly dissolved, stirred and cooled, and then discharged to obtain a Meixin ball solvent solution.

[0210] (3) Use a pipette to add 3 mL of the Meixinqiu solvent solution into the vial containing 1.0 g of the compound essential oil efficacy core ball steady-state delivery system B6;

[0211] (4) Gently shake the compound freeze-dried functional core ball steady-state delivery system to dissolve in the solvent liquid, and the solvent liquid emulsifies the essential oil to obtain an essence emulsion. After standing for 5 minutes, the essence emulsion partially floats up to obtain the milk cap type anthocyanin-Vc compound functional core ball steady-state delivery system essence emulsion VI (see Figure 6 Essence lotion, C6); for subsequent evaluation.

[0212] Application Example 9 Anthocyanin-Vc Complex Efficacy Core Ball Steady-state Delivery System Essence Emulsion VII

[0213] (1) Weigh 1.0 g of anthocyanin-Vc composite essential oil functional core ball steady-state delivery system B7 (including 0.95 g of essential oil and 0.05 g of A7 functional core ball) and place it into a vial;

[0214] (2) Meixin ball solvent configuration:

[0215] 93.0 g of purified water, 1.5 g of Tween 80, 0.7 g of laureth-4, 0.5 g of xylitol glucoside, 0.6 g of anhydroxylitol, 0.5 g of sodium hyaluronate, 1.6 g of polyglycerol-10, 0.8 g of p-hydroxyacetophenone, and 0.8 g of hexylene glycol were weighed in sequence and placed in a container; heated at 40° C. with stirring until uniformly dissolved, stirred and cooled, and then discharged to obtain a Meixin ball solvent solution.

[0216] (3) Use a pipette to add 3 mL of the Meixinqiu solvent solution into the vial containing 1.0 g of the compound essential oil efficacy core ball steady-state delivery system B7;

[0217] (4) Gently shake the compound freeze-dried functional core ball steady-state delivery system to dissolve in the solvent liquid, and the solvent liquid emulsifies the essential oil to obtain an essence emulsion. After standing for 5 minutes, the essence emulsion partially floats up to obtain the milk cap type anthocyanin-Vc compound functional core ball steady-state delivery system essence emulsion VII (see Figure 6 Essence lotion, C7); for subsequent evaluation.

[0218] Application Example 10 EUK134 Control Essence

[0219] (1) Weigh 86.7 g of purified water, 5.0 g of butylene glycol, 0.80 g of Chondrus crispus, and 0.40 g of raspberry ketone in sequence, place them in a container, heat at 40°C, and stir until dissolved evenly;

[0220] (2) After stirring and cooling, add 5.0 g of Rosa damascena floral water, 2.0 g of 1,2-pentanediol, and 0.10 g of EUK134 in sequence and stir for about 20 minutes to obtain a uniform solution. The material can be discharged for subsequent evaluation.

[0221] Application Example 11 EUK134 Complex Functional Core Ball Steady-State Delivery System Essence Emulsion VIII

[0222] (1) Weigh 1.0 g of EUK134 compound essential oil functional core ball steady-state delivery system B8 (including 0.95 g of essential oil and 0.05 g of A8 functional core ball) and place it into a vial;

[0223] (2) Meixin ball solvent configuration:

[0224] 94.5 g of purified water, 2.0 g of butanediol, 1.5 g of xylitol, 0.6 g of betaine, 0.5 g of xanthan gum, 0.4 g of glycerol polyether-26, 2.0 g of β-glucan, 0.3 g of glycosyl trehalose, 0.6 g of p-hydroxyacetophenone, and 1.0 g of hexylene glycol were weighed in sequence and placed in a container; heated at 40° C. and stirred to dissolve uniformly; after stirring and cooling, the material was discharged to obtain a Meixinqiu solvent solution.

[0225] (3) Use a pipette to add 3 mL of the Meixinqiu solvent solution into the vial containing 1.0 g of the compound essential oil efficacy core ball steady-state delivery system B8;

[0226] (4) Gently shake the compound freeze-dried functional core ball steady-state delivery system to dissolve in the solvent liquid, and the solvent liquid emulsifies the essential oil to obtain an essence lotion. After standing for 5 minutes, the essence lotion partially floats up to obtain a double-layer transparent EUK134 compound functional core ball steady-state delivery system essence lotion VIII (see Figure 6 Essence lotion, C8); for subsequent evaluation.

[0227] Application Example 12 EUK134 Complex Functional Core Ball Steady-State Delivery System Essence Emulsion IX

[0228] (1) Weigh 1.0 g of EUK134 compound essential oil functional core ball steady-state delivery system B9 (including 0.95 g of essential oil and 0.05 g of A9 functional core ball) and place it into a vial;

[0229] (2) Meixin ball solvent configuration:

[0230] 94.5 g of purified water, 2.0 g of butanediol, 1.5 g of xylitol, 0.6 g of betaine, 0.5 g of xanthan gum, 0.4 g of glycerol polyether-26, 2.0 g of β-glucan, 0.3 g of glycosyl trehalose, 0.6 g of p-hydroxyacetophenone, and 1.0 g of hexylene glycol were weighed in sequence and placed in a container; heated at 40° C. and stirred to dissolve uniformly; after stirring and cooling, the material was discharged to obtain a Meixinqiu solvent solution.

[0231] (3) Use a pipette to add 3 mL of the Meixinqiu solvent solution into the vial containing 1.0 g of the compound essential oil efficacy core ball steady-state delivery system B9;

[0232] (4) Gently shake the compound freeze-dried functional core ball steady-state delivery system to dissolve in the solvent liquid, and the solvent liquid emulsifies the essential oil to obtain the essence lotion. After standing for 5 minutes, the essence lotion partially floats up to obtain the double-layer transparent EUK134 compound functional core ball steady-state delivery system essence lotion IX (see Figure 6 Essence lotion, C9); for subsequent evaluation.

[0233] Application Example 13: Blue Copper Peptide Control Essence

[0234] (1) Weigh 86.6 g of purified water, 5.0 g of butylene glycol, 0.80 g of Chondrus crispus, and 0.40 g of raspberry ketone in sequence, place them in a container, and heat at 40°C with stirring until they are evenly dissolved;

[0235] (2) After stirring and cooling, add 5.0g of Rosa Damascena floral water, 2.0g of 1,2-pentanediol, and 0.20g of blue copper peptide in sequence, and stir for about 10 minutes to obtain a uniform solution. The material can be discharged for subsequent evaluation.

[0236] Application Example 14: Blue Copper Peptide Complex Efficacy Core Ball Steady-State Delivery System Essence Lotion X

[0237] (1) Weigh 1.0 g of the blue copper peptide compound essential oil functional core ball steady-state delivery system B10 (including 0.95 g of essential oil and 0.05 g of A10 functional core ball) and place it into a vial;

[0238] (2) Meixin ball solvent configuration:

[0239] Weigh 91.1 g of purified water, 2.0 g of butylene glycol, 1.2 g of xylitol, 2.0 g of glycerol, 0.5 g of sodium hyaluronate, 1.0 g of β-glucan, 0.5 g of glycosyl trehalose, 0.7 g of p-hydroxyacetophenone, and 1.0 g of pentylene glycol in sequence and place them in a container; heat and stir at 40°C to dissolve evenly, stir and cool, then discharge the material to obtain the Meixinqiu solvent solution.

[0240] (3) Use a pipette to add 3 mL of the Meixinqiu solvent solution into a vial containing 1.0 g of the compound essential oil efficacy core ball steady-state delivery system B10;

[0241] (4) Gently shake the compound freeze-dried functional core ball steady-state delivery system to dissolve in the solvent liquid, and the solvent liquid emulsifies the essential oil to obtain the essence lotion. After standing for 5 minutes, the essence lotion partially floats up to obtain a double-layer transparent blue copper peptide compound functional core ball steady-state delivery system essence lotion X (see Figure 6 Essence lotion, C10); for subsequent evaluation.

[0242] Application Example 15: Blue Copper Peptide Complex Efficacy Core Ball Steady-State Delivery System Essence Lotion XI

[0243] (1) Weigh 1.0 g of the blue copper peptide compound essential oil functional core ball steady-state delivery system B11 (including 0.95 g of essential oil and 0.05 g of A11 functional core ball) and place it into a vial;

[0244] (2) Meixin ball solvent configuration:

[0245] 91.1 g of purified water, 2.0 g of butylene glycol, 1.2 g of xylitol, 2.0 g of glycerol, 0.5 g of sodium hyaluronate, 1.0 g of β-glucan, 0.5 g of glycosyl trehalose, 0.7 g of p-hydroxyacetophenone, and 1.0 g of pentylene glycol were weighed in sequence and placed in a container; heated at 40° C. and stirred to dissolve uniformly; after stirring and cooling, the material was discharged to obtain a Meixinqiu solvent solution.

[0246] (3) Use a pipette to add 3 mL of the Meixinqiu solvent solution into the vial containing 1.0 g of the compound essential oil efficacy core ball steady-state delivery system B11;

[0247] (4) Gently shake the compound freeze-dried functional core ball steady-state delivery system and dissolve it in the solvent. At the same time, the solvent will emulsify the essential oil to obtain an essence lotion. After 5 minutes, the essence lotion will partially float up to obtain a double-layer transparent blue copper peptide compound functional core ball steady-state delivery system essence lotion XI (see Figure 6 Essence lotion, C11); for subsequent evaluation.

[0248] The above embodiments illustrate the basic principles, main features, and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The above embodiments and the contents described in the specification are merely illustrative of the principles of the present invention. Various changes and improvements may be made to the present invention without departing from the scope of the principles of the present invention, and all such changes and improvements fall within the scope of protection of the present invention.

Claims

1. A compound essential oil efficacy core ball steady-state delivery system, characterized in that: include: Unstable small molecule active ingredients, biosynthetic active macromolecules, penetration enhancer molecules, functional small molecules and essential oils; the unstable small molecule active ingredients are selected from one or more of ergothioneine, glutathione, anthocyanidin, blue copper peptide, vitamin C, ethylbisiminomethyl elm alkaloid manganese chloride active ingredients; the penetration enhancer molecules are selected from one or more of phloretin, menthol, hydroxypropyl β-cyclodextrin, methyl β-cyclodextrin, and butylene glycol; the unstable small molecule active ingredients, biosynthetic active macromolecules, penetration enhancer molecules and functional small molecules The mass ratio of the active ingredients is 1:0.1-10:0.05-5:0.1-10; the particle size of the composite essential oil functional core ball is in the range of 0.1-15 mm; the composite essential oil functional core ball stable delivery system is prepared by the following process: A. uniformly mixing the unstable small molecule active raw material and the biosynthetic active macromolecule, then adding purified water and stirring to dissolve to obtain a clear and transparent solution; B. adding the penetration enhancer molecule and the functional small molecule to the above solution during the stirring process, stirring until all components are completely dissolved to obtain the target solution; C. Add the target solution dropwise into an insulated liquid nitrogen bucket; during the addition, nitrogen gas needs to be introduced into the target solution to protect it from oxidation and loss of the active ingredients; D. Freeze-dry the small ice balls formed by freezing the liquid nitrogen to obtain composite freeze-dried functional core balls; E. Disperse the functional core balls into the essential oil, which will quickly fill the pores of the functional core balls to obtain composite essential oil functional core balls.

2. The compound essential oil efficacy core ball steady-state delivery system according to claim 1, characterized in that: The biosynthetic active macromolecule is selected from one or more of fermented polysaccharides, fermented proteins, recombinant collagen, recombinant type III collagen, recombinant type XII collagen, recombinant fibronectin, yeast protein, polyglutamic acid, pullulan, yeast polysaccharide, yeast β-glucan, sodium hyaluronate, xanthan gum, gellan gum, sodium DNA, and fermented Ganoderma lucidum polysaccharide components.

3. The compound essential oil efficacy core ball steady-state delivery system according to claim 1, characterized in that: The functional small molecule is selected from one or more of the active ingredients of madecassoside, asiaticoside, theanine, swalnic acid, arbutin, nicotinamide, tranexamic acid, ectoine, Vc glucoside, catechin, trehalose, nonapeptide-1, snake venom peptide, conopeptide, acetyl hexapeptide-8, and mannitol.

4. The compound essential oil efficacy core ball steady-state delivery system according to claim 1, characterized in that: The essential oil is selected from one or more of coconut oil caprylate / caprate, isohexadecane, isododecane, jojoba seed oil, squalane, fermented peony seed oil, fermented sacha inchi oil, fermented rice bran oil, fermented grape seed oil, fermented vegetable oil, sphaeranthus serrata seed butter, corn germ oil, tocopheryl acetate, rapeseed oil, macadamia nut seed oil, isononyl isononanoate, ethylhexyl palmitate, caprylic / capric triglyceride, meadowfoam seed oil, sunflower seed oil, rosa damascena flower oil, hydrogenated polyisobutene, dimethicone, lavender essential oil, tea tree essential oil, plant essential oil, and essence.

5. A method for preparing a functional skin care essence, characterized in that: The method comprises the following steps: mixing a solvent liquid with the composite essence oil functional core ball steady-state delivery system according to any one of claims 1 to 4, gently shaking and mixing to obtain a freshly prepared functional skin care essence liquid; the solvent liquid comprises water, a moisturizer and / or an emulsifier.

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