A fully automatic multi-chip microbial online monitoring system

Abstract

The present invention discloses a fully automatic multi-chip online microbial monitoring system; it includes a clamping module, a chip to be tested storage and conveying module, and a discarded chip storage module; the chip to be tested storage and conveying module includes a chip to be tested slot, in which the chip to be tested is placed; the clamping module is arranged on a synchronous pulley structure, and the clamping module is used to automatically clamp the chip to be tested placed on the chip to be tested slot lifted by the jacking module, and to release the microfluidic chip into the discarded chip slot of the discarded chip storage module. Through the mutual coordination between different modules and optimized program control, the present invention automatically completes the storage, grabbing, positioning, detection, discarding and other tasks of multiple chips on a single device. The application scenario is not limited. Without the need for manual operation, the air microbial data of the test interval can be continuously obtained, thereby improving the detection efficiency.

Description

Technical Field

[0001] The present invention relates to the technical field of microbial detection, and in particular to a full-automatic multi-chip microbial online monitoring system. Background Art

[0002] In closed environments such as large shopping malls, cinemas, hospitals and farms, long-term automatic sampling and detection of pathogenic microorganisms in the air can be used to generate monitoring data of pathogenic microorganisms in the air over a certain period of time, thereby achieving monitoring and early warning of environmental safety. This allows for immediate disinfection measures before a disease breaks out, reducing the incidence of the disease and preventing its spread and transmission.

[0003] When it is necessary to obtain data on air pathogenic microorganisms in an environment at different time periods in order to monitor the environment over a long period of time, it is necessary to test the collected air samples in different time periods. If the existing air microorganism detection system is to detect air samples over a continuous period of time, it can only manually replace the reaction container and reaction reagent after completing the pathogenic microorganism detection of an air sample each time. This is not only time-consuming and labor-intensive, but also has low detection efficiency and high cost. That is, the existing microbial detection system is unable to fully automate the long-term detection and monitoring of air microorganism samples in different time periods in the environment, and is unable to fully automate the detection of samples in different time periods. It can only analyze one sample at a time and relies on manual replacement of reaction containers and reaction reagents, which is time-consuming and labor-intensive, with low detection efficiency and high cost. Therefore, there is an urgent need to develop a fully automated online microbial monitoring system that can detect samples in different time periods. Summary of the Invention

[0004] To address the technical problem in the existing technology of being unable to fully automate the long-term detection and monitoring of air pathogenic microorganism samples over different time periods, the present invention aims to provide a fully automatic multi-chip online microbial monitoring system. By rationally setting the system modules and rationally designing the control process, the present invention completes fully automatic multi-chip detection through the coordination and linkage of various modules.

[0005] The technical solution of the present invention is specifically described as follows.

[0006] The present invention provides a fully automatic multi-chip online microbial monitoring system, comprising a clamping module, a fully automatic single-chip detection system, a test chip storage and conveying module, a lifting module, and a discarded chip storage module. The fully automatic single-chip detection system is positioned opposite the clamping module. The test chip storage and conveying module includes a test chip slot, in which the test chip is placed. The clamping module is mounted on a synchronous pulley structure and is used to automatically clamp the test chip placed in the test chip slot, which has been lifted by the lifting module, and to release the microfluidic chip into the discarded chip slot of the discarded chip storage module. The fully automatic single-chip detection system, in conjunction with the microfluidic chip, performs sample testing.

[0007] In the present invention, the clamping module includes a first clamping jaw, a first connecting rod transmission mechanism, a second clamping jaw and a clamping jaw base. The two ends of the first connecting rod transmission mechanism are fixedly connected to the first clamping jaw and the second clamping jaw respectively. The motor drives the first clamping jaw to make the clamping module perform reciprocating linear motion relative to the clamping jaw base.

[0008] In the present invention, the clamping module is slidably arranged on a first horizontally arranged support member, the first support member is installed on a first horizontally arranged mounting plate through a second support member vertically arranged downward from the middle position, and the first mounting plate is arranged above the second mounting plate; when detecting the chip to be tested, the clamping base of the clamping module that clamps the chip to be tested is located in the middle position of the first support member. In the present invention, the lifting module includes a lifting plate, a lifting column, a connecting member, and a second crank-connecting rod mechanism; one end of the second crank-connecting rod mechanism is connected to the motor drive end, and the other end passes through a limiting channel installed on the connecting member on the lifting plate and is fixedly arranged on the lifting plate, the lifting column is fixedly connected to both ends of the lifting plate, and the lifting column is used to lift and support the microfluidic chip in the chip slot to be tested through the second mounting plate to receive discarded chips.

[0009] The present invention further comprises an air sampler, which is mounted on a third mounting plate fixedly connected to the second support member in a liftable manner, and is arranged close to the first support member.

[0010] The present invention also includes a detection and heating module; the detection and heating module includes a second connecting rod transmission mechanism composed of a push rod, a first linkage rod and a push plate, a first guide column and a detection and heating mechanism; the detection and heating mechanism is installed on the detection and heating mechanism mounting plate, one end of the first guide column is fixedly connected to the limit plate, and the other end forms a movable connection with the detection and heating mechanism mounting plate, a spring is installed on the first guide column connecting the limit plate and the detection and heating mechanism mounting plate, the push rod is fixedly connected to the fully automatic single-chip detection system arranged on the first mounting plate through a sliding slider, the push plate is fixedly connected to the detection and heating mechanism mounting plate, one end of the first linkage rod is rotatably connected to one end of the push plate, and the other end abuts against the push rod; when working, the fully automatic single-chip detection system drives the push rod to slide, the push rod pushes the first linkage rod, and the first linkage rod further drives the push plate to push the detection and heating mechanism mounting plate close to the limit plate compression spring. At this time, the detection and heating mechanism approaches the chip to be tested together with the detection and heating mechanism mounting plate.

[0011] In the present invention, the fully automatic single-chip detection system includes a base plate, a cam mechanism, a clamping module for driving the cam mechanism to move back and forth linearly, and an on-off component for controlling the on and off of the flow channel of the microfluidic chip; the cam mechanism is fixedly connected to the clamping module, the clamping module is slidably set on the base plate, the on-off component is movably set on the clamping module, the cam mechanism is in contact with the on-off component, and the on-off component is fixedly set on the clamping module.

[0012] In the present invention, the storage and transportation module for chips to be tested comprises a first crank-connecting rod mechanism, a refrigeration box body, a first refrigeration box cover, a second refrigeration box cover and a slot for chips to be tested;

[0013] The motor driving end is connected to the first cold storage box cover through a first crank-connecting rod mechanism, the first cold storage box cover is movably connected to the cold storage box body through a connecting rod structure, and the second cold storage box cover and the chip slot to be tested are fixedly arranged into a module; the chip slot to be tested is slidably set on the second guide column through a linear bearing, and the second cold storage box cover and the chip slot to be tested perform reciprocating linear motion on the second guide column. When the chip slot to be tested moves linearly into the cold storage box body, the first cold storage box cover and the second cold storage box cover can form a closed structure with the cold storage box body.

[0014] In the present invention, the waste chip slot is linked to the chip conveying module to be tested and the waste chip slot through the second linkage rod and the second synchronous belt; the motor drives the second linkage rod to rotate, and the second synchronous belt drives the second cold storage box cover and the chip slot to be tested to perform reciprocating linear motion on the second guide column.

[0015] The present invention also includes a number of correspondingly arranged photoelectric sensors and induction sheets, as well as a cloud intercommunication module; the cloud intercommunication module is electrically connected to the controller.

[0016] The present invention also provides a fully automatic multi-chip microbial online monitoring method, comprising the following steps:

[0017] 1) Place the chips into the chip slots to be tested one by one;

[0018] 2) Control the chip storage module to store the chip in the cold storage box;

[0019] 3) Start the clamping module and move the first clamp, the second clamp, and the clamp base to a specific position to wait for the chip to be grasped;

[0020] 4) Control the cold storage box to open and move the chip to be tested under the gripper;

[0021] 5) Control the lifting mechanism to rise and lift the chip into the clamping jaws;

[0022] 6) Control the first clamp, the second clamp, and the clamp base to clamp the chip and move the chip to the inspection station;

[0023] 7) Control the lifting mechanism to descend, control the chip slot to be tested to return to the cold storage box, and close the first cold storage box cover and the second cold storage box cover;

[0024] 8) Control the fully automatic single-chip detection system to move close to the chip, and the heating module is linked to stick to the chip;

[0025] 9) Control the air sampler to descend and dock with the sampling port on the chip to start sampling;

[0026] 10) Start the fully automatic single-chip detection system to test the sample. When the test experiment reaches the heating step, the controller obtains reaction information through the detection module and saves and processes the experimental data. After the test experiment is completed, the controller sends both the processed experimental data and the original data to the cloud. The cloud obtains, saves, and further processes the experimental data. The web page, mini program, and mobile app obtain data through the cloud. Staff can monitor the test data in real time through the web page, mini program, and mobile app.

[0027] 11) Control the air sampler to rise and separate from the chip sampling port;

[0028] 12) Control the fully automatic single chip detection system to move away from the chip and release the chip;

[0029] 13) Control the discarded chip slot to move to a specific position and wait;

[0030] 14) Control the first clamp, the second clamp, and the clamp base to move to the discarded chip slot, control the lifting mechanism to rise, drag the chip, and control the first clamp, the second clamp, and the clamp base to release the chip;

[0031] 16) Control the lifting mechanism to descend, and the discarded chips are freely placed in the chip slot to be tested;

[0032] 17) Control the discarded chip slot to move to the origin;

[0033] 18) Control the first gripper, the second gripper, and the gripper base to return to the origin to complete the test process;

[0034] 19) Repeat the above steps when you need to test the second and third chips.

[0035] Compared with the existing technology, the beneficial effect of the present invention is that: through the mutual coordination between different modules and optimized program control, the present invention automatically completes the storage, capture, positioning, detection, discarding and other tasks of multiple chips on a single device. The application scenarios are not restricted. Without manual operation, the air microbial data of the test interval can be continuously obtained, which improves the detection efficiency and solves the technical problem of how to fully automate the long-term detection and monitoring of multiple air pathogenic microorganism samples in scenarios such as farms within different time periods. BRIEF DESCRIPTION OF THE DRAWINGS

[0036] Figure 1 Schematic diagram of the clamping module structure Figure 1 .

[0037] Figure 2 Schematic diagram of the clamping module structure Figure 2 .

[0038] Figure 3 It is the structure of the fully automatic multi-chip microbial online detection system Figure 1 .

[0039] Figure 4 Schematic diagram of the sampling module structure Figure 1 .

[0040] Figure 5 Schematic diagram of the sampling module structure Figure 2 .

[0041] Figure 6 for Figure 5 A partial enlarged view of .

[0042] Figure 7 Schematic diagram of the clamping module structure Figure 3 .

[0043] Figure 8 This is a structural diagram of the chip delivery module to be tested.

[0044] Figure 9 It is the structure of the fully automatic multi-chip microbial online detection system Figure 2 .

[0045] Figure 10 This is the structural diagram of the fully automatic multi-chip microbial online detection system without the clamping module, sampling module, and cam detection mechanism.

[0046] Figure 11 It is the structure of the fully automatic multi-chip microbial online detection system Figure 3 .

[0047] Figure 12 This is a cross-sectional view of the refrigerated container.

[0048] Figure 13 Schematic diagram of the lifting module structure Figure 1 .

[0049] Figure 14 Schematic diagram of the lifting module structure Figure 2 .

[0050] Figure 15 This is a structural diagram of a fully automatic multi-chip microbial online detection system with some modules omitted.

[0051] Figure 16 Schematic diagram of the structure of the fully automatic single-chip detection system.

[0052] Numbers in the figure:

[0053] 1-first clamping jaw, 2-second clamping jaw, 3-clamping jaw base, 4-screw motor, 5-first support member, 6-second support member, 7-first stepper motor, 8-first synchronous wheel, 9-first synchronous belt, 10-screw nut, 11-first connecting rod transmission mechanism, 12-first mounting plate, 13-second mounting plate, 14-third support member, 15-linear guide rail, 16-slider, 17-air sampler, 18-crank, 19-connecting transmission rod, 20-third mounting plate, 21-fourth mounting plate, 22-second stepper motor, 23-turntable, 24-notch, 25-detection heating mechanism, 26-push rod, 27-first linkage rod, 28-push plate, 29-first guide column, 30-detection heating mechanism mounting plate, 31-limiting plate, 32-spring, 33-refrigeration box body, 34-first refrigerator cover, 35-connecting rod structure, 36-chip slot to be tested, 37-chip to be tested, 38-third stepping motor, 39-fourth support member, 40-second guide column, 41-first crank-connecting rod mechanism, 42-belt, 43-linear bearing, 44-fourth stepping motor, 45-second refrigerator cover, 46-second synchronous wheel, 47-plastic insulation layer, 48-refrigeration plate, 49-metal surface, 50-fixed seat, 51-second linkage rod, 52-second Synchronous belt, 53-waste chip slot, 54-waste chip storage module, 55-synchronous belt pressure plate, 56-first photoelectric sensor, 57-first sensor plate, 58-second photoelectric sensor, 59-lifting plate, 60-lifting column, 61-third guide column, 62-second linear bearing, 63-connecting part, 64-limiting channel, 65-fifth stepper motor, 66-third photoelectric sensor, 67-third sensor plate, 68-fourth photoelectric sensor, 69-fourth sensor plate, 70-second crank-connecting rod mechanism, 71-fifth photoelectric sensor, 72-fifth sensor plate, 73-sixth photoelectric sensor, 74-seventh photoelectric sensor, 75-bottom plate, 76-cam mechanism, 77-side plate, 78-needle plate, 79-on-off assembly. DETAILED DESCRIPTION

[0054] The technical solution of the present invention is described in detail below with reference to the accompanying drawings.

[0055] like Figure 1-Figure 7 As shown, the present invention provides a fully automatic multi-chip microbial online monitoring system, which includes a clamping module, a sampling module, a detection module + a heating module, a storage and transportation module for chips to be tested, and a waste chip storage module.

[0056] Clamping module: used to automatically clamp or release the microfluidic chip into the chip slot to be tested.

[0057] The clamping module includes: a first clamping jaw 1, a second clamping jaw 2, a clamping jaw base 3, a screw motor 4, a first support member 5, a second support member 6, a first stepper motor 7, a first synchronous wheel 8, a first synchronous belt 9, a first connecting rod transmission mechanism 11, a first mounting plate 12, a second mounting plate 13, a third support member 14, a linear guide 15, a slider 16, a fifth photoelectric sensor 71, and a fifth sensor sheet 72; the first synchronous wheel 8 is fixedly arranged at the driving end of the first stepper motor 7, the first synchronous wheel 8 and the first synchronous belt 9 cooperate for transmission, the first clamping jaw 1, ... The two clamping jaws 2 and the clamping jaw base 3 are respectively fixedly connected to the corresponding sliders 16, the slider 16 is slidably set on the linear guide 15, the linear guide 15 is fixedly set on the first support member 5, the clamping jaw base 3 is fixedly connected to the first synchronous belt 9, one end of the first connecting rod transmission mechanism 11 is fixedly connected to the first clamping jaw 1, the other end of the first connecting rod transmission mechanism 11 is fixedly connected to the second clamping jaw 2, the first clamping jaw 1 is fixedly set on the driving end of the screw motor 4, the screw nut 4 is fixedly set on the clamping jaw base 3, and the fifth photoelectric sensor 71 is fixedly set on the first On the support member 5, the fifth induction plate 72 is fixedly arranged on the clamping jaw base 3; the first stepper motor 7 drives the first synchronous wheel 8 to rotate, the first synchronous belt 9 and the first synchronous wheel 8 cooperate to transmit, and the first synchronous belt 9 drives the clamping jaw base 3, the first clamping jaw 1 and the second clamping jaw 2 to transmit; when the screw motor 4 is running, the driving end of the screw motor 4 makes a reciprocating linear motion relative to the clamping jaw base 3, and the screw nut 10 and the clamping jaw base 3 are stationary relative to the first clamping jaw 1 and the second clamping jaw 2; the first clamping jaw 1, the second clamping jaw 2 and the clamping jaw base 3 are connected through the first connecting rod transmission mechanism 1 1 realizes clamping or loosening of the microfluidic chip. The driving end of the screw motor 4 drives the first clamping jaw 1 away from the clamping jaw base 3, while the first connecting rod transmission mechanism 11 drives the second clamping jaw 2 away from the clamping jaw base 3. At this time, the first clamping jaw 1 and the second clamping jaw 2 are both away from the clamping jaw base 3, and the microfluidic chip is loosened. The driving end of the screw motor 4 drives the first clamping jaw 1 close to the clamping jaw base 3, while the first connecting rod transmission mechanism 11 drives the second clamping jaw 2 close to the clamping jaw base 3. At this time, the first clamping jaw 1 and the second clamping jaw 2 are both close to the clamping jaw base 3, and the microfluidic chip is clamped.

[0058] Sampling module: used to collect air samples.

[0059] The sampling module includes: a linear guide 15, a slider 16, an air sampler 17, a crank 18, a connecting drive rod 19, a third mounting plate 20, a fourth mounting plate 21, a second stepping motor 22, and also includes a sixth photoelectric sensor 73, a seventh photoelectric sensor 74, and a turntable 23; the sixth photoelectric sensor 73 and the seventh photoelectric sensor 74 are fixedly arranged on the second stepping motor 22, the turntable 23 is movably arranged at the driving end of the second stepping motor 22, and a notch 24 is provided on the turntable 23. The air sampler 17 is fixedly arranged on the fourth mounting plate 21, the second stepping motor 22 is fixedly arranged on the third mounting plate 20, and the fourth mounting plate 21 and the slider 16 are fixedly arranged. Fixedly connected, the slider 16 is slidably set on the linear guide 15, the linear guide 15 is fixedly set on the third mounting plate 20, the third mounting plate 20 is fixedly set on the second support 6, the driving end of the second stepper motor 22 is fixedly connected to the crank 18, the crank 18 is rotatably set on the third mounting plate 20, the crank 18 is movably connected to the connecting transmission rod 19, and the connecting transmission rod 19 is movably connected to the fourth mounting plate 21; the second stepper motor 22 drives the crank 18 to perform circular motion, and the crank 18 drives the connecting transmission rod 19 to move and further drives the fourth mounting plate 21 to reciprocate linearly on the linear guide 15, thereby driving the air sampler 17 to rise and fall.

[0060] Detection and heating module: used to obtain color information in the microfluidic chip. The detection submodule is a color sensor, a fluorescence detector, or an image recognition module.

[0061] The detection and heating module includes: a second connecting rod transmission mechanism composed of a push rod 26, a first linkage rod 27 and a push plate 28, a first guide column 29, a detection and heating mechanism mounting plate 30, a limit plate 31, and a spring 32; the fixed end of the first linkage rod 27 is rotatably set on the second support member 6, one end of the first linkage rod 27 is rotatably connected to one end of the push plate 28, the other end of the first linkage rod 27 abuts against the push rod 26, the detection and heating mechanism mounting plate 30 is fixedly connected to the push plate 28, the detection and heating mechanism mounting plate 30 is movably set on the first guide column 29, and the limit plate 31 is fixed to one end of the first guide column 29 The spring 32 is fixedly connected to the first guide post 29 between the limit plate 31 and the detection and heating mechanism mounting plate 30. The detection and heating mechanism 25 is fixedly mounted on the detection and heating mechanism mounting plate 30. The push rod 26 is fixedly connected to the cam group puncture mechanism (the cam group puncture mechanism is the fully automatic single-chip detection system). The cam group puncture mechanism is slidably mounted on the first mounting plate 12 via a slider, and the first mounting plate 12 is fixedly mounted on the second mounting plate 13. The push rod 26 is fixedly connected to the fully automatic single-chip detection system. This connection transmission method can save a motor, simplifying the structure and control. The cam group puncture mechanism drives the push rod 26 to slide toward the second support member 6. The push rod 26 pushes the first linkage rod 27, which further drives the push plate 28 to push the detection and heating mechanism mounting plate 30 close to the limit plate 31 to compress the spring 32. At this time, the detection and heating mechanism 25 and the detection and heating mechanism mounting plate 30 approach the microfluidic chip.

[0062] like Figure 16 As shown, the fully automatic single-chip detection system includes: a base plate 75, a cam mechanism 76, a clamping module for driving the cam mechanism to move back and forth linearly (the side plate 77 and the needle plate 78 constitute the clamping module), and an on-off component 79 for controlling the on and off of the flow channel of the microfluidic chip; the cam mechanism 76 is fixedly connected to the clamping module, the clamping module is slidably set on the base plate 75, the on-off component 79 is movably set on the clamping module, the cam mechanism 76 is in contact with the on-off component 79, and the on-off component 79 is fixedly set on the clamping module; when working, the clamping module drives the cam mechanism 76 and the on-off component 79 to move linearly close to the microfluidic chip, and the cam mechanism 75 controls the on-off component 79 in turn to open or close the flow channel on the microfluidic chip, and controls the solution to be transferred to the target chamber for microbial detection experiments.

[0063] The test chip storage and conveying module includes: a third stepper motor 38, a fourth stepper motor 44, a first crank-connecting rod mechanism 41, a cold storage box body 33, a first cold storage box cover 34, a second cold storage box cover 45, a connecting rod structure 35, a test chip slot 36, a test chip 37, a second guide post 40, a second synchronous wheel 46, a second synchronous belt 52, a synchronous belt pressure plate 55, a second linkage rod 27, a fixing base 50, a first photoelectric sensor 56, a second photoelectric sensor 58, and a first sensing plate 57;

[0064] The chip to be tested 37 is placed in the chip to be tested slot 36, and the chip to be tested slot 36 is slidably set on the second guide column 40 through a linear bearing 43. One end of the second guide column 40 is fixedly connected to the inside of the cold storage box 33, and the other end of the second guide column 40 is fixedly connected to the fourth support member 39. The cold storage box 33 and the fourth support member 39 are fixedly set on the second mounting plate 13. The driving end of the third stepper motor 38 is connected to the first cold storage box cover 34 through the first crank-connecting rod mechanism 41. The first cold storage box cover 34 is movably connected to the cold storage box 33 through the connecting rod 35 structure. The second cold storage box cover 45 and the chip to be tested slot 36 are fixedly set to form a module. The second cold storage box cover 45 is fixedly connected to the second synchronous belt 52 through the synchronous belt pressure plate 55. (Such a design of the cold storage box cover realizes the sealing of the cold storage box within a shorter stroke, reduces the space of the cold storage box cover, and makes the device (Compact equipment structure) The fourth stepper motor 44 is fixedly set on the second mounting plate 13, the second linkage rod 27 passes through the fixing base 50 and is rotatably connected to the fixing base 50, the fixing base 50 is fixedly connected to the second mounting plate 13, one end of the second linkage rod 27 is fixedly connected to the driving end of the fourth stepper motor 44, a second synchronous belt 52 is set between the fourth stepper motor 44 and the end of the second linkage rod 27, and a second synchronous belt 52 for transmitting the discarded chip slot 54 is provided between the other end of the second linkage rod 27 and the fixing base 50, a second photoelectric sensor 58 is fixedly set on the second mounting plate 13, and a first sensor plate 57 is fixedly set below the second refrigerator cover, and when the second refrigerator cover 45 closes the refrigerator body, the second refrigerator cover 45 is just above the second photoelectric sensor 58, and the first sensor plate 57 enters the groove of the second photoelectric sensor 58;

[0065] The fourth stepper motor 44 drives the second linkage rod 27 to rotate. At the same time, the second synchronous belt 52 drives the second cold storage box cover 45 and the chip slot 36 to perform reciprocating linear motion on the second guide column 40; the chip slot 36 to be tested is set in the cold storage box. When the experiment is needed, the third stepper motor 38 and the fourth stepper motor 44 simultaneously drive the first cold storage box and the second cold storage box to open, and the fourth stepper motor 44 drives the second synchronous belt 52 to drive the second cold storage box cover 45 and the chip slot 36 to leave the cold storage box. After grabbing the chip to be tested, the third stepper motor 38 drives the first cold storage box cover 34 to cover the upper half of the cold storage box, and the fourth stepper motor 44 synchronously drives the second synchronous belt 52 to drive the chip slot 36 to enter the cold storage box 33 until the second cold storage box cover 45 covers the lower half of the cold storage box 33. At this time, the first cold storage box cover 34 and the second cold storage box cover 45 tightly cover the cold storage box 33, and the cold storage box 33 is isolated from the outside.

[0066] Internal structure of the refrigeration box: A plastic insulation layer 48 is provided inside the outer shell of the refrigeration box 33, and a refrigeration plate 47 is provided inside the outer shell of the refrigeration box 33. The refrigeration end of the refrigeration plate 47 is connected to the metal surface 49. The metal refrigeration effect is good and the internal space of the refrigeration box 33 can be cooled more quickly.

[0067] The waste chip storage module 54 includes: a waste chip slot 53 and waste chips;

[0068] The discarded chip tray 53 is mounted on the second mounting plate 13 via a fourth support member 39. A single fourth stepper motor 44 is required to simultaneously link the chip transport module to be tested and the discarded chip tray 53 via the second linkage rod 27 and the second synchronous belt 52. Linking the discarded chip tray 53 with the chip tray 36 can save motors and simplify the structure. Both ends of the second linkage rod 27 are in transmission connection with the belt 42, and the bottom of the discarded chip tray 53 is fixedly connected to the belt 42. When the chips to be tested need to be removed from the cold storage chamber 33 for testing, the fourth stepper motor 44 drives the second linkage rod 27 to rotate, driving the belt 42. The belt 42 drives the chip tray 36 to slide on the second guide post 40 and out of the cold storage chamber 33. Simultaneously, the second linkage rod 27 rotates, driving the belt 42 to drive the chip discard module to slide on the second guide post 40. In a specific embodiment, the chips to be tested can employ the chip to be tested structure disclosed in Chinese patent application CN119657250A.

[0069] The lifting module includes: a lifting plate 59, a lifting column 60, a third guide column 61, a connecting piece 63, a fifth stepping motor 65, a third photoelectric sensor 66, a third sensing sheet 67, a fourth photoelectric sensor 68, a fourth sensing sheet 69, and a second crank-connecting rod mechanism 70;

[0070] The fifth stepper motor 65 is fixedly arranged on the second mounting plate 13, the connecting member 63 is fixedly arranged on the lifting plate 59, and the connecting member 63 is provided with a limiting channel 64 for the rotation of the second crank-connecting rod mechanism 70. One end of the second crank-connecting rod mechanism 70 is connected to the driving end of the fifth stepper motor 65, and the other end of the second crank-connecting rod mechanism 70 passes through the limiting channel 64 and is fixedly arranged on the lifting plate 59. The lifting plate 59 is slidably arranged on the third guide column 61 through a linear bearing 62. The third guide column 61 is fixedly arranged on the second mounting plate 13. Both ends of the lifting plate 59 are fixedly connected to the lifting column 60. The lifting column 60 can freely pass through the second mounting plate 13. The third photoelectric sensor 66 The fourth photoelectric sensor 68 is arranged on the second mounting plate 13 through a bracket, and the third sensing plate 67 and the fourth sensing plate 69 are fixedly arranged on the lifting plate 59; when detection is required, the fifth stepper motor 65 drives the second crank-connecting rod mechanism 70 to move in the limiting channel 64 to drive the lifting plate 59 to make a linear motion in the direction close to the second mounting plate 13. At the same time, the lifting column 60 fixedly connected to the lifting plate 59 also makes a linear motion close to the microfluidic chip, thereby raising the position of the microfluidic chip to a preset position, which is convenient for the clamping mechanism to grasp, until the third sensing plate 67 rising to the lifting column 60 falls into the groove of the third photoelectric sensor 66, and the controller controls the motor to stop rotating.

[0071] Workflow:

[0072] Before testing the chip 37 to be tested: the controller controls the third stepper motor 38 to start, and the third stepper motor 38 drives the connecting rod mechanism 35 to rotate and drive the first refrigerator cover 34 to open. When the third stepper motor 38 drives the connecting rod mechanism 35 to drive the first refrigerator cover 34 to open to the preset stroke, the controller controls the third stepper motor 38 to turn off; the controller controls the fourth stepper motor 44 to start, and the fourth stepper motor 44 drives the second linkage rod 27 to rotate and drive the synchronous belt 52 to drive the chip slot 36 to be tested and the first ... The second refrigeration box cover 45 slides out from the refrigeration box body 33, and the fourth stepper motor 44 controls the chip slot 36 to slide on the second guide column 40 to a preset distance. The controller controls the fourth stepper motor 44 to turn off (the first photoelectric sensor 56 is used for extreme protection to prevent the chip slot 36 from sliding beyond the preset distance. When the chip slot 36 slides and drives the first sensor 57 to enter the first photoelectric sensor 56, the controller receives the signal and controls the fourth stepper motor 44 to turn off); pull out the chip slot 36 to be tested, and put the chip to be tested into the refrigeration box body 33. The chips are placed in the chip slot 36 to be tested, and then the chip slot 36 to be tested is clamped on the second guide column 40; the controller controls the fourth stepper motor 44 to start, and the fourth stepper motor 44 drives the second linkage rod 27 to rotate and drive the synchronous belt 52 to drive, and the synchronous belt 52 drives the chip slot 36 to be tested, the chip to be tested 37 and the second refrigerator cover 45 to slide into the refrigerator body 33 until the first sensor sheet 57 enters the groove of the second photoelectric sensor 58. At this time, the second refrigerator cover 45 covers part of the refrigerator body 33, and the controller receives After the first sensing plate 57 receives the signal from the second photoelectric sensor 58, it controls the fourth stepper motor 44 to turn off; then the controller controls the third stepper motor 38 to start, and the third stepper motor 38 drives the connecting rod mechanism 45 to rotate and drive the first cold storage box lid 34 to close. The controller controls the third stepper motor 38 to turn off. At this time, the first cold storage box lid 34 tightly covers the entire cold storage box body 33. The interior of the cold storage box body 33 is isolated from the outside world by the first cold storage box lid 34 and the second cold storage box lid 45. The chip to be tested 37 is stored at a low temperature in the cold storage box body 33.

[0073] Equipment operation process: Steps for automatic air sampling

[0074] Step 1: Move the first and second clamping jaws 1, 2 and the clamping jaw base 3 to above the chip under test 37: The controller controls the first stepper motor 7 to start, and the first stepper motor 7 drives the first synchronous wheel 8 to rotate. The first synchronous belt 9 cooperates with the first synchronous wheel 8 to transmit the power. The first synchronous belt 9 drives the clamping jaw base 3, the first clamping jaw 1 and the second clamping jaw 2 to slide the gripping groove. When the motor controls the gripping groove to slide to a preset distance, the controller controls the first stepper motor 7 to turn off. At this time, the gripping groove formed by the first and second clamping jaws 1, 2 and the clamping jaw base 3 is just above the chip under test 37;

[0075] Step 2: Move the chip to be tested 37 to the bottom of the gripping groove formed by the first and second clamping jaws 1, 2 and the clamping jaw base 3: The controller controls the third stepper motor 38 to start, and the third stepper motor 38 drives the connecting rod mechanism 35 to rotate and drive the first cold storage box cover 34 to open. When the third stepper motor 38 drives the connecting rod mechanism 35 to drive the first cold storage box cover 34 to open to the preset stroke, the controller controls the third stepper motor 38 to turn off; The controller controls the fourth stepper motor 44 to start, and the fourth stepper motor 44 drives the second linkage rod 27 to rotate and drive the synchronous belt 52 The synchronous belt 52 drives the chip slot 36 to be tested and the second cold storage box cover 45 to slide out of the cold storage box body 33. When the fourth stepper motor 44 drives the chip 37 to slide to a preset distance, the controller controls the fourth stepper motor 44 to turn off. At this time, the chip 37 to be tested is just below the gripping groove formed by the first and second clamping jaws 1, 2 and the clamping jaw base 3, and the chip 37 to be tested is just above the lifting column 60. The first photoelectric sensor 56 is used to find the origin, and the other photoelectric sensor serves as an extreme protection to prevent overtravel.

[0076] Step 3: The lifting module lifts the chip to be tested 37 into the gripping groove formed by the first clamping jaw 1, the clamping jaw base and the second clamping jaw: the controller controls the fifth stepper motor 65 to start, and the fifth stepper motor 65 drives the second crank-connecting rod mechanism 70 to move in the limiting channel 64, driving the lifting plate 59 to make a linear motion in the direction close to the second mounting plate 13, until the third sensing piece 67 on the lifting column 60 falls into the groove of the third photoelectric sensor 66. The controller controls the fifth stepper motor 65 to stop. At the same time, the lifting column 60 fixedly connected to the lifting plate 59 rises linearly to lift the chip to be tested 37 above into the gripping groove formed by the first clamping jaw 1, the clamping jaw base 3 and the second clamping jaw 2.

[0077] Step 4: Clamp the chip 37 to be tested and move it to the testing station: the controller controls the screw motor 4 to start, and the driving end of the screw motor 4 and the first clamping jaw 1 move linearly toward the clamping jaw base 3.

[0078] At the same time, the first connecting rod transmission mechanism 11 drives the second clamping jaw 2 to approach the clamping jaw base 3 to clamp the chip to be tested 37, the screw motor 4 controls the clamping jaw to move to a preset distance, and the controller controls the screw motor 4 to turn off. During this process, the screw nut 10 and the clamping jaw base 3 are stationary relative to the first clamping jaw 1 and the second clamping jaw 2; the controller controls the first stepper motor 7 to start, the first stepper motor 7 drives the first synchronous wheel 8 to rotate, the first synchronous belt 9 cooperates with the first synchronous wheel 8 to transmit, and the first synchronous belt 9 drives the clamping jaw base 3, the first clamping jaw 1, the second clamping jaw 2 and the chip to be tested 37 to slide to the cracking chamber of the chip to be tested 3 just below the air sampler 17. At this time, the first stepper motor 7 controls the grasping groove to slide to the predicted distance, and the controller controls the first stepper motor 7 to turn off;

[0079] Step 5: The lifting column 60 descends, and the chip slot 36 to be tested slides into the cold storage box 33: The controller controls the fifth stepper motor 65 to start, and the fifth stepper motor 65 drives the second crank-connecting rod mechanism 70 to move in the limiting channel 64, driving the lifting plate 59 to move linearly away from the second mounting plate 13 until the fourth sensing piece 69 on the lifting column 60 falls into the groove of the fourth photoelectric sensor 68. The controller controls the fifth stepper motor 65 to shut down. At this time, the end surface of the lifting column 60 is lower than the bottom surface of the cold storage box 33.

[0080] The controller controls the fourth stepper motor 44 to start, and the fourth stepper motor 44 drives the second linkage 27 to rotate and drive the synchronous belt 52 to transmit. The synchronous belt 52 drives the chip slot 36 to be tested and the chip 37 to be tested to move to the cold storage box 33, and at the same time drives the second cold storage box cover 45 to approach the cold storage box 33 until the first sensing piece 57 enters the groove of the second photoelectric sensor 58. At this time, the second cold storage box cover 45 covers part of the cold storage box 33. After receiving the signal that the first sensing piece 57 enters the second photoelectric sensor 58, the controller controls the fourth stepper motor 44 to turn off. Then the controller controls the third stepper motor 38 to start, and the third stepper motor 38 drives the connecting rod mechanism 35 to rotate and drive the first cold storage box cover 34 to close. The controller controls the third stepper motor 38 to turn off. At this time, the first cold storage box cover 34 covers the entire cold storage box 33. The interior of the cold storage box 33 is isolated from the outside world by the first cold storage box cover 34 and the second cold storage box cover 45. The remaining chips to be tested 37 are stored at a low temperature in the cold storage box 33.

[0081] Step 6: Positioning, clamping and heating the chip 37 to be tested: The controller controls the cam group puncture mechanism to slide close to the chip 37 to be tested, thereby driving the push rod 26 to slide toward the second support member 6. The push rod 6 pushes the first linkage rod 27, and the first linkage rod 27 further drives the push plate 28 to push the detection and heating mechanism mounting plate 30 close to the limit plate 31 to compress the spring 32. At this time, the detection and heating mechanism 25 and the detection and heating mechanism mounting plate 30 together approach the reaction chamber that finally fits the chip 37 to be tested; the controller controls the cam group puncture mechanism to stop sliding;

[0082] Step 7: The sampler descends to align with the sampling port of the chip to be tested: The controller controls the second stepper motor 22 to turn on, and the second stepper motor 22 drives the crank 18 to perform circular motion. The crank 18 drives the connecting transmission rod 19 to move, driving the fourth mounting plate 21 to move on the linear guide 15 toward the chip to be tested 37, thereby driving the sampler to descend. When the notch 24 on the disk 23 just falls into the groove of the seventh photoelectric sensor 74, the sampler just aligns with the sampling port of the chip to be tested 37. The controller controls the second stepper motor 22 to turn off, start sampling, and capture microorganisms in the air into the lysis chamber of the chip to be tested 37;

[0083] Step 8: The cam assembly puncture mechanism runs the detection program: When working, the pressing module drives the cam mechanism 76 and the on-off assembly 79 to make a linear motion close to the microfluidic chip. The cam mechanism 76 sequentially controls the on-off assembly 79 to open or close the flow channel on the microfluidic chip, controlling the transfer of the solution to the target chamber. Finally, the heating step is started. Under the heating conditions, the nucleic acid reacts with the reaction solution to produce a color reaction. The microbial detection experiment is performed by identifying the color change.

[0084] When the test experiment reaches the heating step, the controller obtains reaction information through the detection module and saves and processes the experimental data. After the test experiment is completed, the controller sends both the processed experimental data and the original data to the cloud, which obtains, saves and further processes the experimental data. The web page, mini program and mobile app obtain data through the cloud, and staff can monitor the test data in real time through the web page, mini program and mobile app.

[0085] Step 9: After the test is completed, the sampler rises: the controller controls the second stepper motor 22 to turn on, and the second stepper motor 22 drives the crank 18 to make a circular motion. The crank 18 drives the connecting transmission rod 10 to move and drive the fourth mounting plate 21 to move away from the chip to be tested 37 on the linear guide 15, thereby driving the sampler to rise. When the notch 24 on the disc 23 just falls into the groove of the sixth photoelectric sensor 73, the sampler is reset, and the controller controls the second stepper motor 22 to turn off;

[0086] Step 10: Release the discarded chips that have completed the inspection process: The controller controls the cam group puncture mechanism to slide away from the chip to be tested 37, thereby driving the push rod 26 to slide away from the second support member 6. The push rod moves away from the first linkage rod 27, and the compressed spring 32 recovers its deformation and releases the pressing force, thereby pushing the inspection and heating mechanism mounting plate 30 away from the discarded chips that have completed the inspection work. At the same time, the inspection and heating mechanism 25 and the inspection and heating mechanism mounting plate 30 move away from the discarded chips that have completed the inspection work. The controller controls the cam group puncture mechanism to stop sliding.

[0087] In step 11, the discarded chip slot 53 is moved out. The controller controls the fourth stepper motor 44 to start, and the fourth stepper motor 44 drives the second linkage rod 27 to rotate, thereby driving the synchronous belt 52 to transmit the energy. The synchronous belt 52 drives the discarded chip slot 53 to slide on the second guide column 40 toward the first support member 5 until the discarded chip slot 53 is just below the gripping groove formed by the first clamping jaw 1, the second clamping jaw 2, and the clamping jaw base 3. The controller controls the fourth stepper motor 44 to stop.

[0088] Step 12: The discarded chip is moved into the discarded chip slot 53: The controller controls the first stepper motor 7 to turn on, and the first stepper motor 7 drives the first synchronous wheel 8 to rotate. The first synchronous belt 9 cooperates with the first synchronous wheel 8 to transmit the power. The first synchronous belt 9 drives the clamping jaw base 3, the first clamping jaw 1, and the second clamping jaw 2 to slide toward the direction of the discarded chip that has completed the inspection work and is close to the first stepper motor 7 until the lower half of the discarded chip is completely entered into the discarded chip slot 53. The controller controls the first stepper motor 7 to turn off;

[0089] The controller starts the fifth stepper motor 65, which drives the second crank-connecting rod mechanism 70 to move within the limiting channel 64, causing the lifting plate 59 to move linearly toward the second mounting plate 13 until the fourth sensing plate 69 on the lifting plate 59 leaves the groove of the fourth photoelectric sensor 68. The controller then turns off the fifth stepper motor 65. At this point, the lifting column 60 is located directly below the discarded chip and supports the discarded chip. The controller then turns off the fifth stepper motor 65.

[0090] The controller controls the screw motor 4 to turn on, and the driving end of the screw motor 4 and the first clamping jaw 1 move linearly away from the clamping jaw base 3. At the same time, the first connecting rod transmission mechanism 11 drives the second clamping jaw 2 away from the clamping jaw base 3 to release the waste chip. The controller controls the screw motor 4 to turn off;

[0091] The controller controls the fifth stepper motor 65 to start, and the fifth stepper motor 65 drives the second crank-connecting rod mechanism 70 to move within the limiting channel 64, causing the lifting plate to perform linear motion in a direction away from the second mounting plate 13, until it descends until the fourth sensing plate 69 on the lifting plate 59 enters the groove of the fourth photoelectric sensor 68. The controller controls the fifth stepper motor 65 to stop. At this time, the end surface of the lifting column 60 is lower than the bottom surface of the discarded chip slot 53, and all the discarded chips enter the discarded chip slot 53. The controller controls the fifth stepper motor 65 to stop.

[0092] Step 13, the discarded chip slot 53 is reset: the controller controls the fourth stepper motor 44 to start, the fourth stepper motor 44 drives the second linkage rod 27 to rotate and drive the second synchronous belt 52 to transmit, and the second synchronous belt 52 drives the discarded chip slot 53 to slide on the second guide column 40 in the direction away from the first support member 5. At the same time, when the first sensing piece 57 on the second cold storage box cover 45 enters the groove of the second photoelectric sensor 58 (through a fourth stepper motor 44, the discarded chip slot 53 and the chip slot to be tested 36 are simultaneously linked to control the sliding of the discarded chip slot 53 and the chip slot to be tested 36 on their respective second guide columns 40, that is, only one fourth stepper motor 44 is needed to remove the chip to be tested 37 from the cold storage box body 33 and reset it, and move the discarded chip slot 53 to the bottom of the clamp and reset it), the discarded chip slot 53 is reset to the initial position, and the controller controls the fourth stepper motor 44 to turn off; the second photoelectric sensor 58 is used to control the return to the origin, and the distance of the stepper motor is used to control the specific moving position;

[0093] Step 14: Reset the first jaw 1, the second jaw 2, and the jaw base 3: The controller controls the first stepper motor 7 to turn on, the first stepper motor 7 drives the first synchronous wheel 8 to rotate, the first synchronous belt 9 cooperates with the first synchronous wheel 8 to transmit, and the first synchronous belt 9 drives the jaw base 3, the first jaw 1, and the second jaw 2 to slide away from the first stepper motor 7 until the fifth sensor plate enters the groove of the fifth sensor 72. The first jaw 1, the second jaw 2, and the jaw base 3 are reset to the initial position, and the controller controls the first stepper motor 7 to turn off. The test is completed.

[0094] When it is necessary to obtain air microbial data again, just repeat the above steps. In this way, when it is necessary to start the air sample test again to obtain air microbial data in different time periods, no manual operation is required. The controller sends instructions to each actuator according to the set program (the above steps are set as a program, and the detection frequency can be set to once a day), and the detection work can be completed fully automatically. Therefore, without the need for manual operation, the system can continuously obtain air microbial data in different time periods to achieve environmental safety monitoring and early warning, so that disinfection measures can be taken immediately before the disease breaks out, reducing the incidence rate and preventing the spread and transmission of the disease.

Claims

1. A fully automatic multi-chip microbial online monitoring system, characterized in that: It includes a clamping module, a fully automatic single-chip detection system, an air sampler, a storage and transportation module for chips to be tested, a lifting module and a waste chip storage module; the fully automatic single-chip detection system is arranged relative to the clamping module, and the clamping module is slidably arranged on a first horizontally arranged support member. When detecting the chip to be tested, the clamping base of the clamping module that clamps the chip to be tested is located in the middle position of the first support member; the storage and transportation module for chips to be tested includes a cold storage box and a chip slot to be tested. The chip to be tested is placed in the chip slot to be tested, and the chip to be tested is stored in the cold storage box at a low temperature; the clamping module is arranged on a synchronous pulley Structurally, the clamping module is used to automatically clamp the chip to be tested placed on the chip slot to be tested that has been lifted by the lifting module, and to release the microfluidic chip into the discarded chip slot of the discarded chip storage module. The air sampler is controlled to descend to dock with the sampling port on the chip, start sampling, and capture microorganisms in the air into the lysis chamber of the chip to be tested. The fully automatic single-chip detection system is equipped with a microfluidic chip to test the sample. The detection and heating module also includes a second connecting rod transmission mechanism consisting of a push rod, a first linkage rod and a push plate, a first guide column and a detection and heating mechanism. The detection and heating mechanism is installed on the detection and heating mechanism mounting plate, one end of the first guide post is fixedly connected to the limit plate, and the other end is movably connected to the detection and heating mechanism mounting plate, a spring is installed on the first guide post connecting the limit plate and the detection and heating mechanism mounting plate, the push rod is fixedly connected to the full-automatic single-chip detection system slidably arranged on the first mounting plate through a slider, the push plate is fixedly connected to the detection and heating mechanism mounting plate, one end of the first linkage rod is rotatably connected to one end of the push plate, and the other end abuts against the push rod; when working, the full-automatic single-chip detection system drives the push rod to slide, and the push rod pushes the first linkage rod , the first linkage rod further drives the push plate to push the detection and heating mechanism mounting plate close to the limit plate compression spring. At this time, the detection and heating mechanism approaches the chip to be tested together with the detection and heating mechanism mounting plate; the fully automatic single-chip detection system includes a base plate, a cam mechanism, a clamping module for driving the cam mechanism to move back and forth linearly, and an on-off component for controlling the on-off of the flow channel of the microfluidic chip; the cam mechanism is fixedly connected to the clamping module, the clamping module is slidably set on the base plate, the on-off component is movably set on the clamping module, the cam mechanism is in contact with the on-off component, and the on-off component is fixedly set on the clamping module.

2. The fully automatic multi-chip online microbial monitoring system according to claim 1, characterized in that: The clamping module includes a first clamping jaw, a first connecting rod transmission mechanism, a second clamping jaw and a clamping jaw base. The two ends of the first connecting rod transmission mechanism are fixedly connected to the first clamping jaw and the second clamping jaw respectively. The motor drives the first clamping jaw to make the clamping module perform reciprocating linear motion relative to the clamping jaw base.

3. The fully automatic multi-chip online microbial monitoring system according to claim 1, characterized in that: The first support member is mounted on a horizontally arranged first mounting plate through a second support member vertically arranged downwardly from a middle position, and the first mounting plate is arranged above the second mounting plate.

4. The fully automatic multi-chip online microbial monitoring system according to claim 3, characterized in that: The lifting module includes a lifting plate, a lifting column, a connecting piece and a second crank-connecting rod mechanism; one end of the second crank-connecting rod mechanism is connected to the motor drive end, and the other end passes through the limiting channel installed on the connecting piece and is fixedly set on the lifting plate. The lifting column is fixedly connected to the two ends of the lifting plate. The lifting column is used to pass through the second mounting plate to lift and support the microfluidic chip in the chip slot to be tested to receive discarded chips.

5. The fully automatic multi-chip online microbial monitoring system according to claim 3, characterized in that: The air sampler is mounted on a third mounting plate fixedly connected to the second supporting member in a liftable manner, and the air sampler is arranged close to the first supporting member.

6. The fully automatic multi-chip online microbial monitoring system according to claim 1, characterized in that: The chip storage and transportation module to be tested includes a first crank-connecting rod mechanism, a cold storage box body, a first cold storage box cover, a second cold storage box cover and a chip slot to be tested; the motor drive end is connected to the first cold storage box cover through the first crank-connecting rod mechanism, the first cold storage box cover is movably connected to the cold storage box body through a connecting rod structure, and the second cold storage box cover and the chip slot to be tested are fixedly arranged into a module; the chip slot to be tested is slidably set on the second guide column through a linear bearing, and the second cold storage box cover and the chip slot to be tested perform reciprocating linear motion on the second guide column. When the chip slot to be tested moves linearly into the cold storage box body, the first cold storage box cover and the second cold storage box cover can form a closed structure with the cold storage box body.

7. The fully automatic multi-chip online microbial monitoring system according to claim 6, characterized in that: The waste chip slot is linked to the chip conveying module to be tested and the waste chip slot through the second linkage rod and the second synchronous belt; the motor drives the second linkage rod to rotate, and the second synchronous belt drives the second cold storage box cover and the chip slot to be tested to perform reciprocating linear motion on the second guide column.

8. The fully automatic multi-chip online microbial monitoring system according to claim 1, characterized in that: It also includes a number of correspondingly arranged photoelectric sensors and induction sheets, as well as a cloud intercommunication module; the cloud intercommunication module is electrically connected to the controller.

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