Production method of gastrodia elata armillaria mellea three-level strain raw material

Abstract

The invention discloses a gastrodia elata armillaria mellea three-level strain raw material production method, and belongs to the technical field of strain production. The method comprises the following steps: mixing and stirring one or more raw materials such as sawdust, bran, gypsum and the like into a culture material, bottling the culture material, then placing a porous plane plastic sheet, compacting and blocking the culture material, and adding 2 centimeters of clear water to create an underwater anoxic environment to inhibit infectious microbes; in a sterile or clean environment, inoculating armillaria mellea secondary mother strain blocks with the diameter of not less than 3.5 cm by using a sterile tool, and then placing the culture bottle in a culture room with the temperature of 22-23 DEG C and the relative humidity of 60%-65% for culture until strains grow to the bottom of the bottle. The method is free of high-temperature sterilization, low in energy consumption, low in cost, capable of effectively reducing infectious microbe pollution and improving strain quality and production efficiency, easy and convenient to operate and suitable for large-scale production.

Description

Technical Field

[0001] The present invention relates to the technical field of strain production, and specifically refers to a raw material production method for the third-level strain of Armillaria mellea for Gastrodia elata Blume Background Art

[0002] Gastrodia elata Blume is a precious Chinese medicinal material, and its growth is closely related to Armillaria mellea. The quality of the Armillaria mellea strain directly affects the yield and quality of Gastrodia elata Blume. Currently, when producing the third-level strain of Armillaria mellea for Gastrodia elata Blume, most rural areas adopt the cooked material production process of sterilizing at 100 °C with atmospheric steam for 10 hours. This production process has the defects of long time consumption, high steam fuel cost and labor cost

[0003] Currently, in the production process of the third-level strain of Armillaria mellea for Gastrodia elata Blume, most adopt the cooked material production process of sterilizing at 100 °C with atmospheric steam for 10 hours. This production process has the defects of long time consumption, high steam fuel cost and labor cost, and moreover, the high-temperature sterilization process may destroy some nutrients in the culture material, affecting the growth and vitality of the strain

[0004] In addition, some existing raw material production methods have problems such as slow growth of the strain, high contamination rate of miscellaneous bacteria, and unstable quality of the strain, making it difficult to meet the needs of large-scale production of the third-level strain of Armillaria mellea for Gastrodia elata Blume

[0005] Therefore, there is an urgent need for a raw material production method for the third-level strain of Armillaria mellea for Gastrodia elata Blume that is efficient, low-cost, and has stable quality Summary of the Invention

[0006] The technical problem to be solved by the present invention is to overcome the above-mentioned technical defects and provide a raw material production method for the third-level strain of Armillaria mellea for Gastrodia elata Blume

[0007] To solve the above technical problem, the technical solution provided by the present invention is a raw material production method for the third-level strain of Armillaria mellea for Gastrodia elata Blume, including the following steps:

[0008] Step 1) Mixing the material: Prepare the culture material and mix it evenly;

[0009] Step 2) Bottling: Fill the evenly mixed culture material into culture bottles;

[0010] Step 3) Placing a partition: After filling the culture material into the bottle, add a porous flat plastic sheet with the same inner diameter as the bottle into the bottle to compact and partition the culture material to prevent the culture material from floating;

[0011] Step 4) Adding water: Add 2 cm of clear water on the plastic sheet to cut off the oxygen passage between the culture material and the outside world with the clear water, so that the culture material is in an underwater anoxic environment;

[0012] Step 5) Inoculation: Inoculate the Armillaria mellea secondary mother culture block on the spacer, making the lower end of the mother culture block closely adhere to the porous flat plastic sheet, and the upper end of the mother culture block is 1 cm above the water level.

[0013] Step 6) Cultivation: Send the inoculated culture bottle into the culture room, control the temperature and relative humidity of the culture room for cultivation until the strain grows to the bottom of the bottle, then the qualified Armillaria mellea tertiary strain is obtained.

[0014] Furthermore, the culture material is a mixture of one or more of sawdust, wheat bran, gypsum, sucrose, cottonseed hulls, corn flour, and superphosphate.

[0015] Furthermore, the inoculation process in Step 5 is carried out in a sterile or relatively clean environment using sterile operating tools.

[0016] Furthermore, the diameter of the Armillaria mellea secondary mother culture block in Step 5 is not less than 3.5 cm.

[0017] Furthermore, in Step 6, the temperature of the culture room is controlled at 22 to 23 degrees, and the relative humidity is controlled at 60% to 65%.

[0018] The advantages of the present invention compared with the prior art are as follows:

[0019] 1) The present invention adopts a raw material production method, without the need for high-temperature sterilization treatment of the culture material, greatly reducing energy consumption and production costs.

[0020] 2) By setting a porous flat plastic sheet and adding water in the culture bottle to form an underwater anoxic environment, the growth of miscellaneous bacteria is effectively inhibited, the contamination rate of miscellaneous bacteria is reduced, and the purity and quality of the strain are improved.

[0021] 3) The reasonable culture material formula and suitable culture conditions can provide sufficient nutrition and a good environment for the growth of Armillaria mellea, accelerating the growth rate of the strain, shortening the production cycle, and improving production efficiency.

[0022] 4) The production method of the present invention is simple to operate and easy to master, suitable for large-scale production of Armillaria mellea tertiary strains for Gastrodia elata, and has good economic and social benefits. Specific Embodiments

[0023] The following further elaborates on a raw material production method for Armillaria mellea tertiary strains for Gastrodia elata of the present invention in combination with embodiments.

[0024] Example 1

[0025] A raw material production method for Armillaria mellea tertiary strains for Gastrodia elata includes the following steps:

[0026] Mixing materials: Weigh 50% sawdust, 30% wheat bran, 1% gypsum, 1% sucrose, 10% cottonseed hulls, 5% corn flour, and 3% superphosphate. Put these raw materials into a blender and stir evenly to obtain the culture medium.

[0027] Bottling: Put the evenly stirred culture medium into the culture bottle, and the filling height is two-thirds of the height of the culture bottle.

[0028] Placing a partition: Add a porous flat plastic sheet with the same inner diameter as the bottle into the bottle, and use tools to compact the culture medium so that the plastic sheet fits tightly with the culture medium to prevent the culture medium from floating.

[0029] Adding water: Add 2 cm of clear water on the plastic sheet to ensure that the clear water can completely cut off the oxygen passage between the culture medium and the outside.

[0030] Inoculation: In the aseptic operation table, use sterile tweezers to insert the Armillaria mellea secondary mother culture block with a diameter of 4 cm onto the partition, making the lower end of the mother culture block closely adhere to the porous flat plastic sheet, and the upper end of the mother culture block is 1 cm higher than the water surface.

[0031] Cultivation: Send the inoculated culture bottle into the cultivation room, control the temperature of the cultivation room at 22 degrees, and the relative humidity at 60%, and carry out cultivation. After 30 days of cultivation, the strain grows to the bottom of the bottle, and qualified Armillaria mellea tertiary seeds are obtained.

[0032] Example 2

[0033] A method for producing Armillaria mellea tertiary seeds for Gastrodia elata by raw material method, including the following steps:

[0034] Mixing materials: Weigh 40% sawdust, 25% wheat bran, 2% gypsum, 2% sucrose, 15% cottonseed hulls, 10% corn flour, and 6% superphosphate. Stir the raw materials evenly to prepare the culture medium.

[0035] Bottling: The same as Example 1.

[0036] Placing a partition: The same as Example 1.

[0037] Adding water: The same as Example 1.

[0038] Inoculation: In a relatively clean environment, use the sterilized operation tools to insert the Armillaria mellea secondary mother culture block with a diameter of 3.8 cm onto the partition, ensuring that the lower end of the mother culture block closely adheres to the porous flat plastic sheet, and the upper end of the mother culture block is 1 cm higher than the water surface.

[0039] Cultivation: Put the culture bottle into the cultivation room, control the temperature of the cultivation room at 23 degrees, and the relative humidity at 65%, cultivate for 30 days, the strain grows to the bottom of the bottle, and qualified Armillaria mellea tertiary seeds are obtained.

[0040] The above describes the present invention and its embodiments, and such description is not restrictive. Generally speaking, if those of ordinary skill in the art are inspired by it and, without departing from the gist of the present invention, design similar structural forms and embodiments to this technical solution without creative efforts, they shall fall within the protection scope of the present invention.

Claims

1. A method for producing raw materials of the third-level strain of Armillaria mellea with Gastrodia elata, characterized in that, It includes the following steps: Step 1) Mixing the materials: Prepare the culture medium and mix it evenly; Step 2) Bottling: Put the evenly mixed culture medium into the culture bottles; Step 3) Placing a partition: After the culture medium is bottled, add a porous flat plastic sheet with the same inner diameter as the bottle into the bottle to compact and partition the culture medium to prevent the culture medium from floating; Step 4) Adding water: Add 2 cm of clear water on the plastic sheet to cut off the oxygen passage between the culture medium and the outside world by using the clear water, so that the culture medium is in an anoxic environment underwater; Step 5) Inoculation: Inoculate the secondary mother culture blocks of Armillaria mellea on the partition sheet, make the lower end of the mother culture block closely adhere to the porous flat plastic sheet, and the upper end of the mother culture block is 1 cm higher than the water surface; Step 6) Cultivation: Send the inoculated culture bottles into the cultivation room, control the temperature and relative humidity of the cultivation room for cultivation until the strain grows to the bottom of the bottle, and then the qualified tertiary strain of Armillaria mellea is obtained.

2. The raw material production method of the third-level strain of Armillaria mellea with Gastrodia elata Blume as claimed in claim 1, wherein: The culture medium is a mixture of one or more of sawdust, wheat bran, gypsum, sucrose, cottonseed hulls, corn flour, and superphosphate.

3. A raw material production method for the third-level strain of Armillaria mellea with Gastrodia elata, characterized in that: The inoculation process in Step 5 is carried out in a sterile or relatively clean environment using sterile operation tools.

4. A raw material production method for the third-level strain of Armillaria mellea with Gastrodia elata Blume, characterized in that: The diameter of the secondary mother culture blocks of Armillaria mellea in Step 5 is not less than 3.5 cm.

5. A raw material production method for the third-level strain of Armillaria mellea with Gastrodia elata Blume, characterized in that: In Step 6, the temperature of the cultivation room is controlled at 22 to 23 degrees, and the relative humidity is controlled at 60% to 65%.