Concrete crack repairing method and system based on silica gel immobilized microorganisms

Abstract

The invention discloses a concrete crack repairing method and system based on silica gel immobilized microorganisms. The method comprises the following steps: preparing a thallus suspension, silica gel and nutritive salt; mixing the thallus suspension with silica gel to form silica gel immobilized with microorganisms; and injecting the concrete crack by using the silica gel immobilized with the microorganisms and nutritive salt to finish repairing the concrete crack. Microorganisms are adsorbed on the surface of the carrier to realize microorganism immobilization, and the high efficiency and environmental protection property of crack repair are improved.

Description

Technical Field

[0001] The present invention relates to the technical field of concrete crack repair, and particularly to a concrete crack repair method and system based on silicone gel immobilized microorganisms. Background Art

[0002] There are various methods for repairing concrete cracks. Currently, the commonly used concrete crack treatment methods in domestic and foreign engineering mainly include surface sealing method, grouting filling method, grooving and caulking method, etc. The most suitable repair plan is selected according to factors such as the width, depth, position of the crack, and the cause of the crack. Surface sealing method: This method is mainly used to repair fine cracks (usually with a width less than 0.2 mm). By applying polymer waterproof agents, permeable waterproof agents and other high molecular waterproof materials on the crack surface to prevent the intrusion of moisture and harmful substances. Grouting filling method: For cracks with a larger width (>0.2 mm) and greater depth, high-strength crack repair materials are directly filled into the cracks under pressure and form a solid filling body after curing to restore the integrity and durability of the component to achieve the repair purpose. Grooving and caulking method: When the crack width is moderate (about 0.5 - 1.5 mm), V-shaped or U-shaped notches can be cut at appropriate positions on both sides of the crack, and then after cleaning, epoxy resin putty or curing agent is filled into the notch and the surface is leveled. Although these traditional crack repair methods have been widely used, the commonly used repair materials are generally organic materials such as epoxy resin and polyurethane. These materials have different thermal expansion coefficients from cement-based materials, poor compatibility, and most organic materials are volatile and will release gases harmful to the human body and the environment. Therefore, there is an urgent need to find an economical, efficient, environmentally friendly and easy-to-operate concrete crack repair material. Summary of the Invention

[0003] The purpose of this part is to outline some aspects of the embodiments of the present invention and briefly introduce some preferred embodiments. Simplifications or omissions may be made in this part, as well as in the abstract and title of the present application, to avoid obscuring the purpose of this part, the abstract and the title. However, such simplifications or omissions shall not be used to limit the scope of the present invention.

[0004] In view of the above existing problems, the present invention is proposed.

[0005] Therefore, the present invention provides a concrete crack repair method and system based on silicone gel immobilized microorganisms to solve the problems of low efficiency and large environmental impact of existing concrete crack repair methods.

[0006] To solve the above technical problems, the present invention provides the following technical solutions:

[0007] In the first aspect, the present invention provides a concrete crack repair method based on silicone gel immobilized microorganisms, including:

[0008] Prepare a cell suspension, silica gel and nutrient salts;

[0009] Mix the cell suspension with the silica gel to form silica gel immobilized with microorganisms;

[0010] Use the silica gel immobilized with microorganisms and nutrient salts to inject the concrete cracks, and complete the repair of the concrete cracks.

[0011] As a preferred embodiment of the concrete crack repair method based on silica gel immobilized microorganisms according to the present invention, wherein:

[0012] Prepare a cell suspension, silica gel and nutrient salts;

[0013] Mix the cell suspension with the silica gel to form silica gel immobilized with microorganisms;

[0014] Use the silica gel immobilized with microorganisms and nutrient salts to inject the concrete cracks, and complete the repair of the concrete cracks.

[0015] As a preferred embodiment of the concrete crack repair method based on silica gel immobilized microorganisms according to the present invention, wherein:

[0016] The preparation of the cell suspension includes the following steps:

[0017] Obtain microorganisms by culturing the bacterial liquid;

[0018] Centrifuge the pre-cultured bacterial liquid at a speed of 0 - 8000 r / min for 5 min to collect the cell sludge, and resuspend the cell sludge with NaCl solution to obtain a cell suspension;

[0019] The culturing of the bacterial liquid includes the following steps:

[0020] Prepare a microbial culture medium: the components of the culture medium include yeast extract, ammonium chloride, manganese sulfate monohydrate, nickel chloride hexahydrate and deionized water; after mixing the components, adjust the pH value of the mixture to 8.5 with 1 mol / L NaOH solution to complete the preparation of the culture medium;

[0021] Aliquot and sterilize the prepared culture medium;

[0022] Use a pipette to aspirate the mother bacterial liquid and add it to the sterilized and cooled culture medium;

[0023] Measure the optical density, and when the OD value reaches 2, the culturing of the bacterial liquid is completed.

[0024] As a preferred embodiment of the concrete crack repair method based on silica gel immobilized microorganisms according to the present invention, wherein:

[0025] The preparation of the silica gel includes the following steps:

[0026] Obtain silica gel with a specific surface area of 200 m 2 / g and a solid content of 30%, and an NaCl solution with a concentration of 60 g / L;

[0027] Mix the silica gel and the NaCl solution in a volume ratio of 5:1 - 1:1, and after standing, obtain the silica gel.

[0028] As a preferred embodiment of the concrete crack repair method based on silica gel immobilized microorganisms of the present invention, wherein:

[0029] The preparation of the nutrient salt includes:

[0030] Prepare a nutrient salt solution using soluble calcium salt and urea, where the concentrations of both the calcium salt and urea are 0.25 - 1 mol / L.

[0031] As a preferred embodiment of the concrete crack repair method based on silica gel immobilized microorganisms of the present invention, wherein:

[0032] The silica gel immobilized with microorganisms includes:

[0033] Stir and mix the centrifuged and resuspended bacterial liquid and the silica gel in a volume ratio of 5:1 - 1:1 to adsorb the microorganisms around the silica gel particles.

[0034] As a preferred embodiment of the concrete crack repair method based on silica gel immobilized microorganisms of the present invention, wherein:

[0035] The injection of the concrete crack using the silica gel immobilized with microorganisms and the nutrient salt includes:

[0036] Adjust the concentration and injection dose of the bacterial suspension according to the width and type of the crack.

[0037] As a preferred embodiment of the concrete crack repair method based on silica gel immobilized microorganisms of the present invention, wherein:

[0038] The injection of the concrete crack using the silica gel immobilized with microorganisms and the nutrient salt includes:

[0039] The microorganisms in the bacterial liquid produce urease that decomposes urea through metabolism;

[0040] Urea is decomposed into ammonia and carbon dioxide. After carbon dioxide dissolves in water, it forms carbonate ions, and after ammonia dissolves in water, the pH value of the solution increases;

[0041] Carbonate ions combine with calcium ions in nutrients to form calcium carbonate crystals, completing the repair of concrete cracks.

[0042] In a second aspect, the present invention provides a concrete crack repair system based on silica gel immobilized microorganisms, comprising:

[0043] A preparation module for preparing a cell suspension, silica gel and nutrients;

[0044] A mixing module for mixing the cell suspension with silica gel to form silica gel immobilized with microorganisms;

[0045] An injection module for injecting the silica gel immobilized with microorganisms and nutrients into concrete cracks to complete the repair of concrete cracks.

[0046] In a third aspect, the present invention provides a computing device, comprising:

[0047] A memory for storing programs;

[0048] A processor for executing the computer-executable instructions, and when the computer-executable instructions are executed by the processor, the steps of the concrete crack repair method based on silica gel immobilized microorganisms are implemented.

[0049] In a fourth aspect, the present invention provides a computer-readable storage medium, comprising: when the program is executed by a processor, the steps of the concrete crack repair method based on silica gel immobilized microorganisms are implemented.

[0050] Advantages of the present invention: This method immobilizes microorganisms by adsorbing them on the surface of the carrier. Compared with the self-repair method, it has the following advantages: 1. Direct action: Since the microorganisms are located on the surface of the carrier, they can directly contact the nutrient solution in the cracks and immediately start the mineralization reaction without waiting for the carrier to rupture. 2. More nucleation sites: The microorganisms adsorbed on the surface of the carrier provide more nucleation sites for the mineralization reaction, which helps to accelerate the deposition of minerals such as calcium carbonate and speed up the repair rate. 3. Strong fluidity: The surface-adsorbed microorganisms and their carriers can better maintain fluidity and are more likely to penetrate into fine and complex crack networks to ensure more comprehensive repair. 4. Instantaneous plugging can be achieved: The carrier of this method can act as a filler to achieve instantaneous plugging of cracks, prevent further expansion, and enhance the integrity of the concrete structure. Description of the Drawings

[0051] To more clearly illustrate the technical solutions of the embodiments of the present invention, the following will briefly introduce the accompanying drawings required for the description of the embodiments. Obviously, the accompanying drawings in the following description are only some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other accompanying drawings can be obtained based on these drawings. Among them:

[0052] Figure 1 It is a schematic diagram of the basic process of a concrete crack repair method based on silica gel-immobilized microorganisms provided by an embodiment of the present invention;

[0053] Figure 2 It is a schematic diagram of immobilized microorganisms of a concrete crack repair method based on silica gel-immobilized microorganisms provided by an embodiment of the present invention;

[0054] Figure 3 It is a comparison chart of the activity of non-immobilized microorganisms and the activity of microorganisms after immobilization of a concrete crack repair method based on silica gel-immobilized microorganisms provided by an embodiment of the present invention;

[0055] Figure 4 It is a comparison chart of the effects of repairing concrete cracks only with microorganisms and after immobilizing microorganisms of a concrete crack repair method based on silica gel-immobilized microorganisms provided by an embodiment of the present invention. Detailed implementation manners

[0056] To make the above objects, features, and advantages of the present invention more obvious and understandable, the following will make a detailed description of the specific implementation manners of the present invention with reference to the accompanying drawings of the specification. Obviously, the described embodiments are part of the embodiments of the present invention, rather than all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0057] Many specific details are set forth in the following description to facilitate a thorough understanding of the present invention, but the present invention can also be implemented in other ways different from those described herein. Those skilled in the art can make similar extensions without departing from the connotation of the present invention. Therefore, the present invention is not limited by the specific embodiments disclosed below.

[0058] Secondly, the so-called "one embodiment" or "embodiment" herein refers to a specific feature, structure, or characteristic that can be included in at least one implementation manner of the present invention. The "in one embodiment" appearing in different places in this specification does not necessarily refer to the same embodiment, nor is it an independent or alternative embodiment that excludes other embodiments.

[0059] The present invention will be described in detail with reference to the schematic diagrams. When describing the embodiments of the present invention in detail, for the convenience of explanation, the cross-sectional views showing the device structure will be enlarged locally in a non-general proportion, and the schematic diagrams are only examples and should not limit the scope of protection of the present invention herein. In addition, in actual production, three-dimensional spatial dimensions including length, width, and depth should be included.

[0060] Meanwhile, in the description of the present invention, it should be noted that the orientation or positional relationship indicated by terms such as "upper, lower, inner, and outer" is based on the orientation or positional relationship shown in the drawings. It is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus should not be construed as a limitation of the present invention. In addition, the terms "first, second, or third" are only used for descriptive purposes and cannot be construed as indicating or implying relative importance.

[0061] Unless otherwise clearly defined and limited in the present invention, the terms "installed, connected, and coupled" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can also be a mechanical connection, an electrical connection, or a direct connection, and can also be indirectly connected through an intermediate medium, or can be the communication inside two elements. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0062] Embodiment 1

[0063] Referring to Figure 1 , an embodiment of the present invention provides a method for repairing concrete cracks based on silicone gel-supported microorganisms, including:

[0064] S1: Prepare a cell suspension, silicone gel, and nutrient salts;

[0065] In the embodiment of the present application, preparing the cell suspension includes the following steps:

[0066] Obtain microorganisms by culturing the bacterial liquid;

[0067] Centrifuge the pre-cultured bacterial liquid at a speed of 0 - 8000 r / min for 5 min to collect the cell sludge, and resuspend the cell sludge with NaCl solution to obtain the cell suspension;

[0068] In the embodiment of the present application, culturing the bacterial liquid includes the following steps:

[0069] Configure a microbial culture medium: The components of the culture medium include yeast extract, ammonium chloride, manganese sulfate monohydrate, nickel chloride hexahydrate, and deionized water; after mixing the components, use 1 mol / L NaOH solution to adjust the pH value of the mixture to 8.5 to complete the preparation of the culture medium;

[0070] Dispense and sterilize the prepared culture medium;

[0071] Use a pipette to aspirate the mother yeast solution and add it to the sterilized and cooled culture medium;

[0072] Measure the optical density. When the OD value reaches 2, the bacterial liquid culture is completed.

[0073] In the embodiment of the present application, preparing silica gel includes the following steps:

[0074] Obtain silica gel with a specific specific surface area of 200 m² / g, a solid content of 30%, and an NaCl solution with a concentration of 60 g / L;

[0075] Mix the silica gel and the NaCl solution in a volume ratio of 5:1 - 1:1. After standing, silica gel is obtained.

[0076] In the embodiment of the present application, preparing nutrient salts includes:

[0077] Prepare a nutrient salt solution using soluble calcium salt and urea, where the concentrations of both the calcium salt and urea are 0.25 - 1 mol / L.

[0078] S2: Mix the bacterial suspension with the silica gel to form silica gel with immobilized microorganisms;

[0079] In the embodiment of the present application, the silica gel with immobilized microorganisms includes:

[0080] Stir and mix the centrifuged and resuspended bacterial liquid with the silica gel in a volume ratio of 5:1 - 1:1, and adsorb the microorganisms around the silica gel particles.

[0081] S3: Use the silica gel with immobilized microorganisms and nutrient salts to inject into the concrete cracks to complete the repair of the concrete cracks.

[0082] In the embodiment of the present application, using the silica gel with immobilized microorganisms and nutrient salts to inject into the concrete cracks includes adjusting the concentration of the bacterial suspension and the injection dose according to the width and type of the cracks.

[0083] In the embodiment of the present application, using the silica gel with immobilized microorganisms and nutrient salts to inject into the concrete cracks includes that the microorganisms in the bacterial liquid produce urease that decomposes urea through metabolism; urea is decomposed into ammonia and carbon dioxide, carbon dioxide dissolves in water to form carbonate ions, and the pH value of the solution increases after ammonia dissolves in water; the carbonate ions further combine with calcium ions in the nutrient salts to form calcium carbonate crystals to complete the repair of the concrete cracks.

[0084] It should be noted that in this method, microorganisms are immobilized by adsorbing them on the surface of the carrier, and natural microorganisms are used for repair, which has less impact on the environment and belongs to the mechanism of microbial deposition of calcium carbonate in green repair technology. Compared with the self-repair method, this method can fix microorganisms at the crack by the immobilized substance, provide nucleation sites for the mineralization reaction of microorganisms inside the concrete crack, and can also act as a crack filler. Moreover, it has strong fluidity and is more likely to penetrate into the fine and complex crack network. While repairing microcracks, it greatly shortens the repair cycle of concrete microcracks and can simultaneously achieve rapid plugging of concrete cracks. This innovation will effectively solve many limitations in the existing technology and provide a new technical means for repairing existing concrete cracks.

[0085] This embodiment also provides a concrete crack repair system based on immobilizing microorganisms with silica gel, including:

[0086] A preparation module for preparing a bacterial suspension, silica gel, and nutrient salts;

[0087] A mixing module for mixing the bacterial suspension with the silica gel to form silica gel immobilizing microorganisms;

[0088] An injection module for injecting the silica gel immobilizing microorganisms and nutrient salts into the concrete crack to complete the repair of the concrete crack.

[0089] Furthermore, it further includes:

[0090] A memory for storing programs;

[0091] A processor for loading the program to execute the above-mentioned concrete crack repair method based on immobilizing microorganisms with silica gel.

[0092] This embodiment also provides a computer-readable storage medium storing a program, which when executed by a processor, implements the above-mentioned concrete crack repair method based on immobilizing microorganisms with silica gel.

[0093] The storage medium proposed in this embodiment and the above-mentioned concrete crack repair method based on immobilizing microorganisms with silica gel belong to the same inventive concept. Technical details not described in detail in this embodiment can be referred to the above embodiment, and this embodiment has the same beneficial effects as the above embodiment.

[0094] From the above description of the embodiments, those skilled in the art can clearly understand that the present invention can be implemented by means of software and necessary general-purpose hardware. Of course, it can also be implemented by hardware, but in many cases, the former is a better implementation manner. Based on such an understanding, the technical solution of the present invention, in essence, or the part that contributes to the prior art, can be embodied in the form of a software product. The computer software product can be stored in a computer-readable storage medium, such as a floppy disk, read-only memory (ROM), random access memory (RAM), flash memory (FLASH), hard disk, or optical disc of a computer, etc., and includes several instructions to enable a computer device (which can be a personal computer, server, or network device, etc.) to execute the methods of various embodiments of the present invention.

[0095] Example 2

[0096] Refer to Figures 2 - 4 , which is an embodiment of the present invention, provides a method for repairing concrete cracks based on silica gel-supported microorganisms. In order to verify its beneficial effects, the comparison results of two schemes are provided.

[0097] The specific embodiments are as follows:

[0098] Step 1: Cultivate the bacterial liquid. The components of the microbial culture medium include yeast extract, ammonium chloride, manganese sulfate monohydrate, nickel chloride hexahydrate, and deionized water. After mixing the components, adjust the pH value to 8.5 with 1 mol / L NaOH to complete the preparation of the culture medium. Dispense the culture solution into 250 ml conical flasks, cover them with breathable sealing membranes and fasten them with rubber bands. Place the filled conical flasks in a pressure steam sterilizer, set the temperature to 121 °C and the time to 20 min, start sterilization. After cooling, place the culture medium in a laminar flow hood, turn on the ultraviolet sterilization lamp and irradiate for 20 min for sterilization treatment. After irradiation, turn on the alcohol lamp to prevent external microorganisms from entering. Use a pipette to aspirate 1 ml of the mother bacterial liquid into the culture medium to complete the inoculation of the culture medium. After microbial inoculation, set the temperature of the shaker to 30 °C and the rotation speed to 150 r / min, and culture for a certain period of time until the liquid culture medium becomes turbid, and a bacterial liquid with an OD value of about 2 can be obtained, and the culture is completed.

[0099] Step 2: Prepare the cell suspension. Centrifuge the overnight-cultured bacterial liquid at a rotation speed of 0 - 8000 r / min for 5 min to collect the cell sludge, preferably at 5000 r / min, and resuspend the cell sludge with an 8.5 g / L NaCl solution to prepare the cell suspension.

[0100] Step 3: Prepare silica gel. Mix silica gel with a specific surface area of 200 m2 / g and a solid content of 30% with a 60 g / L NaCl solution at a volume ratio of 5:1 - 1:1, preferably 1:1. After standing for 2 hours, silica gel is obtained.

[0101] Step 4: Immobilize microorganisms. Stir and mix the resuspended bacterial solution after centrifugation with silica gel at a volume ratio of 5:1 - 1:1, preferably 1:1. Microorganisms will adsorb around the silica gel particles, as Figure 2 shown, and the activity of the immobilized microorganisms is not much different from that of the non-immobilized microorganisms, as Figure 3 shown. Therefore, this carrier has basically no effect on the activity of microorganisms.

[0102] Step 5: Prepare nutrient salts. The nutrient salts are a mixed solution of soluble calcium salts and urea, where the concentrations of both calcium salts and urea are 0.25 - 1 mol / L. After stirring and mixing the resuspended bacterial solution after centrifugation with silica gel at a volume ratio of 1:1, add nutrient salts with a concentration of 0.25 - 1 mol / L for the microorganism for mineralization reaction, preferably at a concentration of 0.75 mol / L.

[0103] Step 6: Fabricate concrete specimens with cracks. The materials for making concrete specimens include 325 Portland cement, sand, and water, and their mixing ratio is 2.5:1:7.5. Weigh a certain amount of concrete materials according to the ratio, mix and stir evenly, and use a square mold with a length, width, and height of 70.7 mm for molding. Demold after 24 hours and conduct standard curing. Take out the concrete specimens from the curing box on the 7th day and place them on the table for 10 - 15 minutes to allow the surface moisture to evaporate. Then place the sample in a press, and place a steel nail or steel bar at the center of the top surface. Start the press to load at a rate of 50 N / s, and stop loading when obvious cracks appear in the specimen. Control the value of each applied load within the range of ±10%. Take out the steel nail, measure and record the crack width and length, and the production of specimens with real cracks is completed.

[0104] Step 7: Repair concrete cracks. Take two prepared specimens, divide them into groups a and b. Inject the crack with the bacterial solution in group a and inject the crack with the immobilized bacterial solution in group b. Inject the crack with an equal amount of nutrient salts three times for both groups a and b, and then inject the crack with the bacterial solution and the immobilized bacterial solution for groups a and b respectively. Repeat the above operations after 24 hours, and repeat the above operations again after 48 hours, and the repair is completed after 72 hours.

[0105] As Figure 4 shown, for the concrete cracks repaired with the materials obtained in this embodiment, when observed with an optical microscope, the cracks repaired with the bacteria plus silica gel carrier in the first group are as Figure 4 shown in a, and the cracks repaired with only the bacterial solution in the second group are as Figure 4As shown in b, after 72 hours of repair, it can be observed through an optical microscope that the repair effect of the second group of cracks is much better than that of the first group.

[0106] Example 3

[0107] This is an embodiment of the present invention, which provides a concrete crack repair method based on silica gel-immobilized microorganisms. In order to verify its beneficial effects, scientific demonstration is carried out through specific implementation methods and implementation results.

[0108] The details of this embodiment are as follows:

[0109] Silica gel and 60 g / L NaCl were fully mixed in a volume ratio of 1:1. After about 2 hours, silica gel was formed. The same volume of bacterial suspension as that of silica gel was added and stirred thoroughly. Soluble calcium salt and urea were stirred and mixed. The concentrations of calcium salt and urea were both 0.75 mol / L to form a nutrient solution.

[0110] The above materials were weighed separately as raw materials. 240 ml of the cultured bacterial solution was centrifuged at 5000 rpm for 5 minutes, the supernatant was discarded, and only the bacterial sludge was retained. The sludge was then resuspended in 24 ml of 8.5 g / L NaCl solution to prepare Solution A. 12 ml of silica gel and 12 ml of 60 g / L NaCl solution were mixed and stirred to prepare Solution B. After standing for 2 hours, the silica gel immobilized with microorganisms was formed. Finally, Solutions A and B were mixed and stirred. 10.4 g of calcium salt and 5.625 g of urea were weighed and added to 100 ml of pure water and stirred to form 100 ml of 0.75 mol / L nutrient solution. The silica gel immobilized with microorganisms was injected into the concrete cracks, followed by the same amount of nutrient solution injected into the cracks (repeated three times). Finally, the silica gel immobilized with microorganisms was injected into the cracks in the same manner. The concrete cracks were repaired again 24 hours later and again 48 hours later.

[0111] It should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present invention may be modified or replaced by equivalents without departing from the spirit and scope of the technical solutions of the present invention, which should all be included in the scope of the claims of the present invention.

Claims

1. A concrete crack repair method based on silicone gel immobilized microorganisms, characterized in that, Including: Preparing a bacterial suspension, silica gel, and nutrient salts; Mixing the bacterial suspension with the silica gel to form silica gel immobilized with microorganisms; Using the silica gel immobilized with microorganisms and the nutrient salts to inject into the concrete cracks to complete the repair of the concrete cracks.

2. The concrete crack repair method based on silicone gel-supported microorganisms according to claim 1, characterized in that: The preparation of the bacterial suspension includes the following steps: Obtaining microorganisms by culturing the bacterial liquid; Centrifuging the pre-cultured bacterial liquid at a rotational speed of 0 - 8000 r / min for 5 min to collect the bacterial sludge, and resuspending the bacterial sludge with an NaCl solution to obtain the bacterial suspension; The culturing of the bacterial liquid includes the following steps: Preparing a microbial culture medium: The components of the culture medium include yeast extract, ammonium chloride, manganese sulfate monohydrate, nickel chloride hexahydrate, and deionized water; After mixing the components, adjusting the pH value of the mixture to 8.5 with 1 mol / L NaOH solution to complete the preparation of the culture medium; Aliquoting and sterilizing the prepared culture medium; Using a pipette to aspirate the mother bacterial liquid and add it to the sterilized and cooled culture medium; Measuring the optical density, and when the OD value reaches 2, the culturing of the bacterial liquid is completed.

3. The method for repairing concrete cracks based on silicone gel-supported microorganisms according to claim 1 or 2, characterized in that: The preparation of the silica gel includes the following steps: Obtain silica gel with a specific specific surface area of 200 m 2 / g, a solid content of 30%, and an NaCl solution with a concentration of 60 g / L; Mixing silica gel with an NaCl solution at a volume ratio of 5:1 - 1:1, and after standing, obtaining the silica gel.

4. The method for repairing concrete cracks based on silicone gel-supported microorganisms according to claim 3, characterized in that: The preparation of the nutrient salts includes: Preparing a nutrient salt solution using soluble calcium salt and urea, where the concentrations of the calcium salt and urea are both 0.25 - 1 mol / L.

5. The method for repairing concrete cracks based on silicone gel-supported microorganisms as described in claim 4, characterized in that: The silica gel immobilized with microorganisms includes: Stirring and mixing the centrifuged and resuspended bacterial liquid with the silica gel at a volume ratio of 5:1 - 1:1 to adsorb the microorganisms around the silica gel particles.

6. The method for repairing concrete cracks based on silicone gel-supported microorganisms according to claim 5, wherein: The use of the silica gel immobilized with microorganisms and the nutrient salts to inject into the concrete cracks includes: Adjusting the concentration and injection dose of the bacterial suspension according to the width and type of the cracks.

7. The method for repairing concrete cracks based on silicone gel-supported microorganisms according to claim 6, characterized in that: The use of the silica gel immobilized with microorganisms and the nutrient salts to inject into the concrete cracks includes: The microorganisms in the bacterial liquid produce urease that decomposes urea through metabolism; Decomposing urea into ammonia and carbon dioxide, the carbon dioxide dissolves in water to form carbonate ions, and the pH value of the solution increases after the ammonia dissolves in water; The carbonate ions combine with calcium ions in the nutrient salts to form calcium carbonate crystals to complete the repair of the concrete cracks.

8. A system for a concrete crack repair method based on silica gel immobilized microorganisms according to claim 1, characterized in that: A preparation module for preparing a bacterial suspension, silica gel, and nutrient salts; A mixing module for mixing the bacterial suspension with the silica gel to form silica gel immobilized with microorganisms; An injection module for using the silica gel immobilized with microorganisms and the nutrient salts to inject into the concrete cracks to complete the repair of the concrete cracks.

9. A computing device, characterized in that, Including: A memory for storing programs; A processor for loading the program to execute the steps of the concrete crack repair method based on silica gel immobilized microorganisms according to any one of claims 1 - 7.

10. A computer-readable storage medium storing a program, characterized in that, When the program is executed by the processor, the steps of the concrete crack repair method based on silica gel immobilized microorganisms according to any one of claims 1 - 7 are implemented.

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