Preparation method of arbuscular mycorrhizal fungus root activating agent

Abstract

The invention relates to the technical field of microbial preparations and plant cultivation, and discloses a preparation method of an arbuscular mycorrhizal fungus root system activator, which comprises the following steps: weighing materials according to a ratio, carrying out pretreatment and disinfection on the materials, and carrying out sterilization and sterilization on the materials to obtain the arbuscular mycorrhizal fungus root system activator, which comprises arbuscular mycorrhizal fungus propagules, natural raw materials, auxiliary materials, nutritional supplements and culture medium components, so as to obtain the arbuscular mycorrhizal fungus root system activator. Auxiliary materials are added into pretreated culture medium components, a nutritional supplement and natural raw materials are fully mixed, the mixed activator material is prepared into particles, the activator particles are placed on a culture medium to be cultured, the growth condition of hyphae is observed, and when the hyphae are interwoven into a net shape, the preparation is successful. By optimizing natural raw materials, auxiliary materials, nutritional supplements and culture medium components, the activity, stability and preparation efficiency of the microbial inoculum can be improved while the technological process is simplified, so that an efficient microbial activator is provided for plant root growth.

Description

Technical Field

[0001] The present invention relates to the technical fields of microbial agents and plant cultivation techniques, and specifically to a preparation method of an arbuscular mycorrhizal fungal root activator. Background Art

[0002] In the prior art, there are some defects in the preparation of arbuscular mycorrhizal fungal root activators. In terms of the culture medium, the acquisition or preparation process of some methods is relatively complex. For example, some methods of obtaining arbuscular mycorrhizal fungal propagules using the soil in the root zone of wild plants as materials require collecting the soil around specific plants and going through multiple processing steps, which is relatively cumbersome to operate and has certain requirements for the collection environment. In addition, some culture methods such as pot culture, although simple to operate and low in cost, have a long production cycle, are easily contaminated, and have low culture efficiency. There are also methods using biochar as the matrix. Although biochar has certain advantages, the hydrothermal carbonization treatment of biochar powder requires specific equipment and conditions, increasing the production cost and technical difficulty.

[0003] In terms of the stability of the activator, the stability of some existing activators needs to be improved during storage and use. For example, some activators in the form of capsules may be affected by external environmental factors during storage or transportation after physical extrusion molding, resulting in problems such as capsule rupture or a decrease in the activity of the activator. These problems limit the large-scale production and application of arbuscular mycorrhizal fungal root activators. Therefore, it is of great significance to develop an arbuscular mycorrhizal fungal root activator that is efficient, stable, and easy to prepare. Summary of the Invention

[0004] (I) Technical Problems to be Solved

[0005] In view of the deficiencies of the prior art, the present invention provides a preparation method of an arbuscular mycorrhizal fungal root activator, which has the advantages of simple operation, low cost, high stability, and high production efficiency, and solves the problems of complex preparation of the culture medium and poor stability of the activator in the existing methods.

[0006] (II) Technical Solutions

[0007] To achieve the above object, the present invention provides the following technical solution: A preparation method of an arbuscular mycorrhizal fungal root activator, comprising the following steps:

[0008] Step 1. Material preparation: Weigh the materials in proportion. The materials include arbuscular mycorrhizal fungal propagules, natural raw materials, auxiliary materials, nutritional supplements, and culture medium components;

[0009] Step 2. Material pretreatment: Perform pretreatment of crushing and sieving on the natural raw materials and culture medium components;

[0010] Step 3. Material disinfection: Disinfect natural raw materials, auxiliary materials, nutritional supplements, and culture medium components under different conditions respectively;

[0011] Step 4. Preparation of the culture medium: Mix the auxiliary materials and distilled water in a ratio of 1:1 to form a solution, add the pretreated culture medium components, and stir evenly;

[0012] Step 5. Preparation of the activator mixture: Mix the nutritional supplements and distilled water in a ratio of 5:3 to form a solution, and fully mix it with the natural raw materials;

[0013] Step 6. Molding: Make the mixed activator materials into particles with a diameter of 0.8 - 1.5 cm;

[0014] Step 7. Cultivation: Place the molded activator particles on the culture medium for cultivation and observe whether it is successful;

[0015] Step 8. Packaging: Pack the successfully prepared activator particles with multi-layer sterile bags;

[0016] Step 9. Storage: Store the packaged activator in a low-temperature, dry, and well-ventilated environment.

[0017] Preferably, the culture medium components and their weight ratios are as follows: straw ferment 36% - 40%; coconut coir 8% - 10%; soybean meal leachate 10% - 13%; perlite 13% - 15%; volcanic stone 10% - 12%; bamboo charcoal particles 8% - 11%.

[0018] Preferably, the natural raw materials and their weight ratios are as follows: humus soil 32% - 35%; volcanic stone 14% - 16%; perlite 8% - 11%; straw powder 20% - 24%; soybean meal 10% - 12%; pine needle powder 2% - 3%; rice husk ash 3% - 5%; bone meal 5% - 7%; shell powder 3% - 6%.

[0019] Preferably, the auxiliary materials and their weight ratios are as follows: humic acid 3.2% - 4.3%; sucrose 2% - 3%; potassium silicate 1% - 2%; magnesium sulfate 1% - 2%; calcium sulfate 1% - 2%; potassium dihydrogen phosphate 1% - 3%; ferrous sulfate 1% - 3%.

[0020] Preferably, the nutritional supplements and their weight ratios are as follows: chitosan oligosaccharide 0.5% - 0.7%; fish protein hydrolysate 2% - 3%; seaweed extract 1.2% - 1.5%; yeast extract 0.9% - 1.3%; amino acid solution 2% - 3%; trace element mixture 1.0% - 1.2%.

[0021] Preferably, the material pretreatment includes the preparation of straw ferment, the soaking of coconut coir, the preparation of soybean meal leachate, and the sieving of perlite, volcanic stone, and bamboo charcoal particles.

[0022] Preferably, the material is disinfected by combining ultraviolet rays and high temperature.

[0023] Preferably, the preparation steps of the culture medium are as follows:

[0024] S4.1. Mix the pretreated straw ferment, coconut coir, perlite, volcanic rock, and bamboo charcoal particles in proportion and stir evenly to form a preliminary mixture.

[0025] S4.2. Add the soybean meal leaching solution to the mixture while stirring, so that the leaching solution completely penetrates into the mixture to form a culture medium matrix with viscosity and looseness.

[0026] S4.3. Dropwise add the prepared auxiliary material solution to the culture medium matrix using a plastic dropper while continuously stirring until various auxiliary materials are evenly distributed in the culture medium.

[0027] Preferably, the mixing and preparation steps of the activator are as follows:

[0028] S5.1. Mix the arbuscular mycorrhizal fungal propagules with the pretreated humus soil, straw powder, soybean meal, pine needle powder, rice husk ash, bone meal, and shell powder in proportion and stir well with a glass rod.

[0029] S5.2. Dropwise add the nutrient supplement to the above mixture while stirring, so that the nutrient supplement is fully integrated with other raw materials.

[0030] S5.3. Further mix the mixed culture medium matrix with natural raw materials and stir evenly to form an activator mixed material.

[0031] Preferably, the cultivation process in step seven is as follows:

[0032] S7.1. Evenly place the formed activator particles in the culture container, with a distance of 3 - 4 cm between the particles.

[0033] S7.2. Place the culture container with the placed activator particles in the culture room, control the temperature of the culture room at 22 - 25°C, keep the humidity at 65% - 70%, and maintain the environmental oxygen content between 21% - 25%.

[0034] S7.3. Control the cultivation duration at 1 - 2 weeks and observe the growth of the activator particles every half hour.

[0035] Compared with the prior art, the present invention provides a preparation method of an arbuscular mycorrhizal fungal root activator, having the following beneficial effects:

[0036] 1. By using pre-fermented straw, coconut coir and bamboo charcoal particles, the present invention can replace the complex field soil screening and multi-step sterilization processes in the traditional method, shortening the preparation time from 6 - 8 hours in the traditional method to 3 - 4 hours, reducing the equipment requirements by 50%, and only requiring a crusher and a stirring device to complete the preparation. This improvement helps to avoid the pollution risk caused by the instability of miscellaneous bacteria and insect eggs in the field soil while reducing production costs and improving production efficiency.

[0037] 2. The present invention achieves dual antibacterial by adding bamboo charcoal particles and pine needle powder, and at the same time uses chitosan coating to further reduce humidity sensitivity, extending the shelf life of the activator from 6 months in the traditional method to 12 months, with an activity retention rate of 90% and a breakage rate reduced to 5%. Compared with the traditional method, the activator of the present invention can better adapt to different environmental conditions and reduce losses during storage and transportation.

[0038] 3. By using straw and coconut coir as raw materials, the present invention reduces the raw material cost while the standardized process can reduce the labor time, thereby further reducing the preparation cost. Compared with the traditional method, the total cost of the present invention is reduced by 10%, with the highest total cost being 18 yuan / kg, while the lowest total cost of the traditional method is 20 yuan / kg. This cost advantage enables the present invention to provide an efficient and low-cost activator. BRIEF DESCRIPTION OF THE DRAWINGS

[0039] Figure 1 It is a flowchart for the preparation of the present invention. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0040] The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all of the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.

[0041] Please refer to Figure 1 , a method for preparing an arbuscular mycorrhizal fungal root activator, comprising the following steps:

[0042] Step 1. Material preparation: Weigh the materials in proportion, and the materials include arbuscular mycorrhizal fungal propagules, natural raw materials, auxiliary materials, nutritional supplements and culture medium components;

[0043] Step 2. Material pretreatment: Perform pretreatment of crushing and sieving on the natural raw materials and culture medium components;

[0044] Step 3. Material disinfection: Disinfect the natural raw materials, auxiliary materials, nutritional supplements and culture medium components under different conditions to ensure sterility;

[0045] Step 4. Preparation of the culture medium: Mix the auxiliary materials and distilled water in a ratio of 1:1 to form a solution, add the pretreated culture medium components, and stir evenly.

[0046] Step 5. Preparation of the activator mixture: Mix the nutritional supplement and distilled water in a ratio of 5:3 to form a solution, and mix it thoroughly with the natural raw materials.

[0047] Step 6. Shaping: Make the mixed activator material into particles with a diameter of 0.8 - 1.5 cm.

[0048] Step 7. Cultivation: Place the shaped activator particles on the culture medium for cultivation and observe whether it is successful.

[0049] Step 8. Packaging: Package the successfully prepared activator particles using a double-layer sterile non-woven bag (inner layer pore size 0.2μm, outer layer moisture-proof film), and seal 500g per bag.

[0050] Step 9. Storage: Store the packaged activator in a low-temperature, dry, and well-ventilated environment, avoiding direct sunlight and high-temperature and high-humidity environments to ensure the activity and stability of the activator.

[0051] Specifically, the culture medium components and their weight ratios are as follows: straw ferment 36% - 40%; coconut coir 8% - 10%; soybean meal leachate 10% - 13%; perlite 13% - 15%; volcanic stone 10% - 12%; bamboo charcoal particles 8% - 11%.

[0052] By using straw and coconut coir as raw materials, the present invention reduces the raw material cost. At the same time, the standardized process can reduce the labor time-consuming, thereby further reducing the preparation cost. Compared with the traditional method, the total cost of the present invention is reduced by 10%. The highest total cost is 18 yuan / kg, while the lowest total cost of the traditional method is 20 yuan / kg. This cost advantage enables the present invention to provide a highly efficient and low-cost activator.

[0053] Specifically, the natural raw materials and their weight ratios are as follows: humus soil 32% - 35%; volcanic stone 14% - 16%; perlite 8% - 11%; straw powder 20% - 24%; soybean meal 10% - 12%; pine needle powder 2% - 3%; rice husk ash 3% - 5%; bone meal 5% - 7%; shell powder 3% - 6%.

[0054] The present invention realizes dual bacteriostasis by adding bamboo charcoal particles and pine needle powder. At the same time, chitosan coating is adopted to further reduce the humidity sensitivity, so that the shelf life of the activator is extended from 6 months of the traditional method to 12 months, the activity retention rate reaches 90%, and the breakage rate is reduced to 5%. Compared with the traditional method, the activator of the present invention can better adapt to different environmental conditions and reduce the loss during storage and transportation.

[0055] Specifically, the auxiliary materials and their weight ratios are as follows: humic acid 3.2% - 4.3%; sucrose 2% - 3%; potassium silicate 1% - 2%; magnesium sulfate 1% - 2%; calcium sulfate 1% - 2%; potassium dihydrogen phosphate 1% - 3%; ferrous sulfate 1% - 3%.

[0056] Specifically, the nutritional supplements and their weight ratios are as follows: chitosan oligosaccharide 0.5% - 0.7%; fish protein hydrolysate 2% - 3%; seaweed extract 1.2% - 1.5%; yeast extract 0.9% - 1.3%; amino acid solution 2% - 3%; trace element mixture 1.0% - 1.2%.

[0057] By using pre-fermented straw, coconut coir and bamboo charcoal particles, the present invention can replace the complex field soil screening and multi-step sterilization processes in the traditional method, shorten the preparation time from 6 - 8 hours in the traditional method to 3 - 4 hours, reduce the equipment requirements by 50%, and only require a crusher and a stirring device to complete the preparation. This improvement helps to avoid the pollution risk caused by the instability of miscellaneous bacteria and insect eggs in the field soil while reducing production costs and improving production efficiency.

[0058] Specifically, the material pretreatment includes the preparation of straw ferment, the soaking of coconut coir, the preparation of soybean meal leaching solution, and the sieving of perlite, volcanic stone and bamboo charcoal particles. The treatment categories include:

[0059] (1) Straw ferment: After cutting the crop straw into sections, add 1% - 2% of fermentation activator, ferment at a temperature of 25 - 30 °C and a humidity of 60% - 65% for 1.5 - 2 months, cover with a breathable film until the straw is fully decomposed. After fermentation is completed, use a crusher to crush the straw ferment to make its particle size suitable, and store it sealed for later use;

[0060] (2) Coconut coir: Soak the coconut coir in clean water for 3 - 6 hours, drain it until the water content is 50% - 55% (no water dripping when pinched by hand) to improve its physical properties;

[0061] (3) Soybean meal leaching solution: Mix soybean meal and water in a ratio of 1:4, heat to 50 - 55 °C, and stir and extract for 1.5 - 2.5 hours while stirring. Then filter it through a 200-mesh filter cloth for 3 - 5 times to remove the residue, and collect the filtrate to obtain a clear soybean meal leaching solution;

[0062] (4) Perlite, volcanic stone, bamboo charcoal particles: Crush perlite and volcanic stone into 1 - 2 mm particles, and keep the bamboo charcoal particles at 3 - 5 mm after sieving, removing the too large or too small particles to ensure uniform particle size.

[0063] Specifically, for material disinfection: an ultraviolet lamp and high temperature are used in combination for disinfection to avoid the residue of chemical disinfectants. The disinfection process is as follows:

[0064] (1) Disinfection of natural raw materials: The humus soil is sterilized with steam at a temperature of 121 - 125 °C for 25 - 30 minutes; the straw powder, soybean meal, and pine needle powder are irradiated with ultraviolet light at a wavelength of 254 nm and an intensity ≥ 100 μW / cm 2 for 20 - 30 minutes; the volcanic rock, perlite, and bamboo charcoal particles are calcined at a high temperature of 200 - 250 °C for 1.5 - 2 hours;

[0065] (2) Disinfection of auxiliary materials and nutritional supplements: The soybean meal leaching solution and amino acid solution are sterilized by pasteurization, with the sterilization temperature set at 65 - 70 °C and the sterilization duration at 25 - 30 minutes; the sucrose and humic acid are placed under an ultraviolet lamp and irradiated for 20 - 30 minutes;

[0066] (3) Disinfection of tools and containers: After wiping the culture containers and stirring tools with 70% - 75% alcohol, they are irradiated with ultraviolet light for 1 - 1.5 hours.

[0067] Specifically, the steps for preparing the culture medium are as follows:

[0068] S4.1. Mix the pretreated straw ferment, coconut coir, perlite, volcanic rock, and bamboo charcoal particles in proportion and stir evenly to form a preliminary mixture;

[0069] S4.2. Add the soybean meal leaching solution to the mixture while stirring, so that the leaching solution completely penetrates into the mixture to ensure uniform overall humidity and form a culture medium matrix with viscosity and looseness;

[0070] S4.3. Use a plastic dropper to gradually add the prepared auxiliary material solution drop by drop to the culture medium matrix while continuously stirring until all auxiliary materials are evenly distributed in the culture medium.

[0071] Specifically, the steps for mixing and preparing the activator are as follows:

[0072] S5.1. Mix the arbuscular mycorrhizal fungal propagules with the pretreated humus soil, straw powder, soybean meal, pine needle powder, rice husk ash, bone meal, and shell powder in proportion and stir well with a glass rod;

[0073] S5.2. Gradually add the nutritional supplement drop by drop to the above mixture while stirring to make the nutritional supplement fully blend with other raw materials;

[0074] S5.3. Further mix the already mixed culture medium matrix with the natural raw materials and stir evenly to form an activator mixture.

[0075] Specifically, the cultivation process in step seven is as follows:

[0076] S7.1. Uniformly place the formed activator particles in a culture container, with a spacing of 3 - 4 cm between the particles to facilitate air circulation and mycelial growth;

[0077] S7.2. Place the culture container with the activator particles in an incubator, control the temperature of the incubator at 22 - 25°C, the humidity at 65% - 70%, and the environmental oxygen content at 21% - 25%;

[0078] S7.3. Control the culture duration at 1 - 2 weeks, observe the growth of the activator particles every half hour. When there is obvious mycelial growth on the surface of the activator particles and the mycelia are intertwined into a network, it indicates that the preparation of the activator is successful.

[0079] By adding bioactive ingredients such as chitosan oligosaccharide (0.6%), hydrolyzed fish protein (3%), and seaweed extract (1.5%), the present invention significantly improves the activity of the microbial agent, increasing the mycelial growth rate from 1.2 mm / day by the traditional method to 2.5 mm / day, the mycelial coverage rate from 50% to 85%, and the effective spore count from 80 million / g to 150 million / g. In addition, the microbial agent of the present invention can significantly promote the growth of plant roots, and the root growth promotion rate and soil fertility improvement rate both reach twice that of the traditional method, which are 40% and 30% respectively, improving the use effect of the microbial agent.

[0080] Example 1 <>

[0081] The components of the culture medium and their weight ratios are as follows: straw ferment 40%; coconut coir 10%; soybean meal leachate 12%; perlite 14%; volcanic rock 11%; bamboo charcoal particles 9%, and the rest is deionized water.

[0083] Comparative Example 1

[0084] The components of the culture medium are as follows: the main component is the soil in the root zone of wild plants, accounting for 60%; 20% of nutrient soil is added; 10% of river sand is used to improve air permeability; 10% of organic fertilizer (not fully decomposed).

[0085] Example 2

[0086] The natural raw materials and their weight ratios are as follows: humus soil 32% - 35%; volcanic rock 14% - 16%; perlite 8% - 11%; straw powder 20% - 24%; soybean meal 10% - 12%; pine needle powder 2% - 3%; rice husk ash 3% - 5%; bone meal 5% - 7%; shell powder 3% - ⑥%.

[0087] Comparative Example 2

[0088] Natural raw materials: Mainly use rhizosphere soil of plants sampled randomly in the wild (without distinguishing plant species), accounting for 50%; add 20% of ordinary garden soil; 10% of weathered rock particles; 10% of untreated straw; 10% of ordinary soybean cake residue.

[0089] Example 3

[0090] Auxiliary materials and their weight ratios are as follows: humic acid 3.6%; sucrose 2.5%; potassium silicate 2%; magnesium sulfate 1.8%; calcium sulfate 1.5%; potassium dihydrogen phosphate 1.6%; ferrous sulfate 2.5%.

[0091] Comparative Example 3

[0092] Auxiliary materials: Only use a small amount of urea (accounting for 1.5%) as a nitrogen source supplement; calcium phosphate (accounting for 2%) as a phosphorus source supplement; no other auxiliary materials such as humic acid and potassium silicate are added.

[0093] Example 4

[0094] Nutritional supplements and their weight ratios are as follows: chitosan oligosaccharide 0.6%; fish protein hydrolysate 3%; seaweed extract 1.5%; yeast extract 1.1%; amino acid solution 2.5%; trace element mixture 1.15%.

[0095] Comparative Example 4

[0096] Nutritional supplements: Use compound fertilizer solution (added after dilution), accounting for 4%, and no bioactive ingredients such as chitosan oligosaccharide, fish protein hydrolysate, and seaweed extract are added.

[0097] To more intuitively show the effects of the examples and comparative examples in solving the problems of the complexity of the preparation of the culture medium matrix and the stability of the activator in the existing methods, the following is the comparison table 1 of the effects of the finished activators prepared according to Examples 1-4 and Comparative Examples 1-4:

[0098] Table 1

[0099]

[0100] The following information is obtained from Table 1:

[0101] 1. Example 1 vs Comparative Example 1: The preparation time is shortened by 50%. Example 1 uses pre-fermented straw (40%) and standardized materials (coconut coir, bamboo charcoal particles), reducing the field soil screening and multi-step sterilization process, and the equipment requirements are reduced by 50%. Comparative Example 1 requires additional equipment (soil separator, organic fertilizer composting pool), while Example 1 only requires a crusher and a stirring device. The technical improvement points of the present invention are obtained: the straw fermented product replaces the field soil, and the bamboo charcoal particles (9%) inhibit miscellaneous bacteria, and no complex sterilization is required.

[0102] 2. The shelf life of the examples is doubled (12 vs 6 months). The bamboo charcoal particles (9%) and pine needle powder (2.5%) have dual antibacterial effects (Comparative Example 1 only relies on unrotted organic fertilizer and is prone to mildew). The chitosan coating (Example 4) reduces humidity sensitivity, and the breakage rate is reduced by 75% (5% vs 20%). Among them, wet granulation (particle porosity ≥ 30%) avoids the problem of fragile capsules in Comparative Example 1.

[0103] 3. The mycelial growth rate of the present invention is increased by 108% (2.5 vs 1.2 mm / day). Among them, chitosan oligosaccharide (0.6%) induces plant symbiotic signals and accelerates mycelial colonization (Comparative Example 4 only uses compound fertilizer and lacks bioactive ingredients). Among them, fish protein hydrolysate (3%) provides a quick-acting nitrogen source and reduces the C / N ratio to 20:1 (the C / N ratio of untreated soybean cake residue in Comparative Example 2 > 30:1). At the same time, the root promotion rate in the examples of the present invention is doubled (40% vs 20%). Among them, seaweed extract (1.5%) contains alginic acid to promote the development of root meristems, while Comparative Example 4 does not have this component.

[0104] 4. The highest total cost of the examples is 18 yuan / kg (raw materials 8 yuan + preparation 10 yuan), and the lowest total cost of the comparative examples: 20 yuan / kg (raw materials 5 yuan + preparation 15 yuan). Therefore, the materials and preparation process of the present invention can save 10% of the total cost: the examples reduce the raw material cost through agricultural waste (straw, coir pith), and the standardized process reduces manual time-consuming.

[0105] It can be seen by comparison that Examples 1-4 are superior to Comparative Examples 1-4 in terms of the complexity of medium preparation, the stability of the activator, the activity and effect of the activator.

[0106] Table 2

[0107]

[0108]

[0109] The medium preparation time of the present invention is shortened to 3-4 hours (the traditional method requires 6-8 hours). The chitosan coating + bamboo charcoal antibacterial makes the shelf life reach 12 months (the traditional activator ≤ 6 months), the mycelial coverage rate is 85% (the traditional method ≤ 50%), the spore number is 150 million / g (the traditional is 80 million / g), the utilization rate of agricultural waste > 60%, avoiding the ecological damage of wild soil mining. The examples solve the defects of the prior art through material recombination (straw ferment, coir pith), functional synergy (chitosan oligosaccharide + fish protein), and process simplification (wet granulation replaces capsule encapsulation).

[0110] Although embodiments of the present invention have been shown and described, it will be understood by those of ordinary skill in the art that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principles and spirit of the present invention, and the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A preparation method of an arbuscular mycorrhizal fungal root activator, characterized in that, It includes the following steps: Step 1, Material preparation: Weigh the materials in proportion. The materials include arbuscular mycorrhizal fungal propagules, natural raw materials, auxiliary materials, nutritional supplements, and culture medium components; Step 2, Material pretreatment: Pretreat the natural raw materials and culture medium components by crushing and sieving; Step 3, Material disinfection: Disinfect the natural raw materials, auxiliary materials, nutritional supplements, and culture medium components under different conditions respectively; Step 4, Preparation of the culture medium: Mix the auxiliary materials and distilled water in a ratio of 1:1 to form a solution, add the pretreated culture medium components, and stir evenly; Step 5, Preparation of the activator mixture: Mix the nutritional supplements and distilled water in a ratio of 5:3 to form a solution, and fully mix it with the natural raw materials; Step 6, Molding: Make the mixed activator materials into particles with a diameter of 0.8 - 1.5 cm; Step 7, Cultivation: Place the molded activator particles on the culture medium for cultivation and observe whether it is successful; Step 8, Packaging: Pack the successfully prepared activator particles with multiple sterile bags; Step 9, Storage: Store the packaged activator in a low-temperature, dry, and well-ventilated environment.

2. The preparation method of an arbuscular mycorrhizal fungal root activator according to claim 1, characterized in that: The culture medium components and their weight ratios are: straw fermented product 36% - 40%; coconut coir 8% - 10%; soybean meal leachate 10% - 13%; perlite 13% - 15%; volcanic stone 10% - 12%; bamboo charcoal particles 8% - 11%.

3. The preparation method of an arbuscular mycorrhizal fungal root activator according to claim 1, characterized in that: The natural raw materials and their weight ratios are: humus soil 32% - 35%; volcanic stone 14% - 16%; perlite 8% - 11%; straw powder 20% - 24%; soybean meal 10% - 12%; pine needle powder 2% - 3%; rice husk ash 3% - 5%; bone meal 5% - 7%; shell powder 3% - 6%.

4. The preparation method of an arbuscular mycorrhizal fungal root activator according to claim 1, characterized in that: The auxiliary materials and their weight ratios are: humic acid 3.2% - 4.3%; sucrose 2% - 3%; potassium silicate 1% - 2%; magnesium sulfate 1% - 2%; calcium sulfate 1% - 2%; potassium dihydrogen phosphate 1% - 3%; ferrous sulfate 1% - 3%.

5. The preparation method of an arbuscular mycorrhizal fungal root activator according to claim 1, characterized in that: The nutritional supplements and their weight ratios are: chitosan oligosaccharide 0.5% - 0.7%; fish protein hydrolyzate 2% - 3%; seaweed extract 1.2% - 1.5%; yeast extract 0.9% - 1.3%; amino acid solution 2% - 3%; trace element mixture 1.0% - 1.2%.

6. The preparation method of an arbuscular mycorrhizal fungal root activator according to claim 1, characterized in that: The material pretreatment includes the preparation of straw fermented product, the soaking of coconut coir, the preparation of soybean meal leachate, and the sieving of perlite, volcanic stone, and bamboo charcoal particles.

7. The preparation method of an arbuscular mycorrhizal fungal root activator according to claim 1, characterized in that: The material disinfection: Adopt a disinfection method combining ultraviolet lamp and high temperature.

8. The preparation method of an arbuscular mycorrhizal fungal root activator according to claim 1, characterized in that: The steps for preparing the culture medium are: S4.1, Mix the pretreated straw fermented product, coconut coir, perlite, volcanic stone, and bamboo charcoal particles in proportion, stir evenly to form a preliminary mixture; S4.2, Add the soybean meal leachate to the mixture while stirring, so that the leachate completely penetrates into the mixture to form a culture medium matrix with viscosity and looseness; S4.3, Use a plastic dropper to gradually add the prepared auxiliary material solution drop by drop into the culture medium matrix, and continuously stir at the same time until all auxiliary materials are evenly distributed in the culture medium.

9. The preparation method of an arbuscular mycorrhizal fungal root activator according to claim 1, characterized in that: The steps for preparing the activator mixture are: S5.

1. Mix the propagules of arbuscular mycorrhizal fungi with the pre-treated humus soil, straw powder, soybean meal, pine needle powder, rice husk ash, bone meal, and shell powder in proportion, and stir well with a glass rod. S5.

2. Slowly add the nutrient supplement drop by drop to the above mixture while stirring to fully integrate the nutrient supplement with other raw materials. S5.

3. Further mix the mixed culture medium with natural raw materials and stir evenly to form an activator mixture.

10. The preparation method of an arbuscular mycorrhizal fungal root activator according to claim 1, characterized in that: The cultivation process in Step 7 is as follows: S7.

1. Evenly place the formed activator particles in the culture container, with a spacing of 3 - 4 cm between the particles. S7.

2. Place the culture container with the activator particles placed in it into the culture room, control the temperature of the culture room at 22 - 25 °C, the humidity at 65% - 70%, and the environmental oxygen content at 21% - 25%. S7.

3. Control the cultivation duration at 1 - 2 weeks and observe the growth of the activator particles every half hour.

Images