Anti-wrinkle firming composition as well as preparation method and application thereof

Abstract

The invention particularly relates to an anti-wrinkle firming composition as well as a preparation method and application thereof, and belongs to the technical field of cosmetics. The composition is prepared from the active ingredients such as the grape seed extract, the nicotinamide, the carnosine, the dipotassium glycyrrhizinate, the ectoine, the caulerpa fimbriata extract and the ascorbic acid polypeptide, the skin elasticity can be effectively improved, the good anti-wrinkle and skin tightening effects are achieved, meanwhile, the safety is high, and no adverse reaction is caused to the skin; the composition disclosed by the invention is simple and convenient to prepare and excellent in effect and has a relatively good application prospect.

Description

Technical Field

[0001] The present invention specifically relates to an anti-wrinkle and firming composition, a preparation method and application thereof, and belongs to the technical field of cosmetics. Background Art

[0002] As skin ages naturally and due to the accumulation of external environmental factors, it gradually experiences collagen loss, reduced elasticity, and deeper wrinkles. Anti-wrinkle and firming cosmetic raw materials or finished products are solutions to these problems. By synergizing multiple active ingredients with cutting-edge technologies, they help delay the signs of aging, improve skin firmness, and reshape a youthful contour.

[0003] The principles of anti-wrinkle and skin firming are multiple, including: stimulating collagen regeneration: using ingredients such as peptides, retinol and its derivatives or extracts to activate fibroblast activity, promote the synthesis of type I and type III collagen and elastic fibers, and improve the skin's support structure from the root; dynamic line inhibition: using peptides or plant and animal extracts to block neuromuscular signal transmission, reduce excessive contraction of facial muscles, and specifically improve dynamic wrinkles in areas such as the eye area and forehead; antioxidant repair: adding vitamins, ergothioneine, resveratrol, or extracts to neutralize the damage of free radicals to cells, delay the process of photoaging, and repair damaged skin barriers; instant lifting and firming: using high-molecular polymers (such as dextran, hyaluronic acid cross-links) to form a breathable mesh membrane on the skin surface, combined with massage techniques to achieve physical lifting, and improve facial lines in the short term.

[0004] Currently, there are many types of anti-wrinkle and firming cosmetic products or raw materials, but the effects are uneven; therefore, it is necessary to provide an anti-wrinkle and firming composition with stable performance and multi-target effects and apply it in cosmetics. Summary of the Invention

[0005] Based on the purpose of solving the above technical problems, the inventors proposed the technical content of the present invention, which specifically relates to an anti-wrinkle and firming composition and its preparation method and application.

[0006] The invention relates to an anti-wrinkle and firming composition, which comprises the following raw materials: grape seed extract, nicotinamide, carnosine, dipotassium glycyrrhizate, ectoine, fernleaf algae extract, and ascorbic acid polypeptide.

[0007] Preferably, the composition comprises the following raw materials, in parts by weight: 20-40 parts of grape seed extract, 10-30 parts of niacinamide, 10-30 parts of carnosine, 5-15 parts of dipotassium glycyrrhizate, 10-30 parts of tetrahydropyrimidine carboxylic acid, 20-40 parts of fernleaf algae extract, and 10-20 parts of ascorbic acid polypeptide.

[0008] Preferably, in the composition, based on parts by weight, the raw material composition further contains 50 - 500 parts of polyol and 200 - 3000 parts of water.

[0009] Preferably, the polyol includes at least one of ethylene glycol, propylene glycol, butylene glycol, pentylene glycol, hexylene glycol, glycerol, polyethylene glycol, and octylene glycol.

[0010] Preferably, in the composition, based on parts by weight, the raw material composition is: 20 - 40 parts of grape seed extract, 10 - 30 parts of niacinamide, 10 - 30 parts of carnosine, 5 - 15 parts of dipotassium glycyrrhizinate, 10 - 30 parts of ectoine, 20 - 40 parts of Caulerpa lentillifera extract, 10 - 20 parts of ascorbyl polypeptide, 50 - 500 parts of polyol, and 200 - 3000 parts of water.

[0011] The present invention also provides a preparation method of the above composition, which includes: heating and stirring the raw materials, standing for aging, sterilizing, and filling.

[0012] The present invention also provides a preparation method of grape seed extract, including: crushing grape seeds, adding water and heating and stirring, then cooling to obtain a product, adding bromelain for enzymolysis, inactivating the enzyme, filtering, concentrating, and drying to obtain grape seed extract. The enzymolysis process of bromelain can significantly improve the types and concentrations of active ingredients in grape seed extract compared with other enzymolysis processes, and increase the anti - wrinkle and firming effect of the preparation on the skin.

[0013] Preferably, the grape seed extract prepared by the above preparation method is used for the preparation of the above composition.

[0014] Preferably, in the preparation method of grape seed extract, the weight ratio of grape seeds to water is 1:10 - 100.

[0015] Preferably, in the preparation method of grape seed extract, the heating and stirring conditions are: heating to 40 - 70 °C and keeping stirring for 2 - 10 hours.

[0016] Preferably, in the preparation method of grape seed extract, it is cooled to 10 - 35 °C.

[0017] Preferably, in the preparation method of grape seed extract, the amount of bromelain added to the product is 200 - 800 u / g.

[0018] Preferably, in the preparation method of grape seed extract, the enzymolysis temperature and time are 40 - 60 °C and 0.5 - 4 hours.

[0019] The present invention also provides a method for preparing a Caulerpa taxifolia extract, which includes: pulverizing Caulerpa taxifolia, adding water and glucose and stirring, sterilizing, then adding Lactobacillus crispatus SHMCC D51652 to obtain a material, then performing anaerobic fermentation, sterilizing, filtering, concentrating and drying to obtain the Caulerpa taxifolia extract. The present invention uses the specific Lactobacillus crispatus SHMCC D51652 to optimize the performance and effect of the extract, and increase its anti-wrinkle and firming effect on the skin in cosmetic raw materials or finished products.

[0020] Preferably, the Caulerpa taxifolia extract prepared by the above preparation method is used for the preparation of the above composition.

[0021] Preferably, in the method for preparing the Caulerpa taxifolia extract, the weight ratio of Caulerpa taxifolia to water is 1:10 - 100.

[0022] Preferably, in the method for preparing the Caulerpa taxifolia extract, the concentration of the added glucose in the material is 1 - 4 wt%.

[0023] Preferably, in the method for preparing the Caulerpa taxifolia extract, the concentration of the added Lactobacillus crispatus SHMCC D51652 in the material is 10 10 -10 12 cells / g.

[0024] Preferably, in the method for preparing the Caulerpa taxifolia extract, the anaerobic fermentation temperature is 20 - 37 °C and the time is 3 - 5 days.

[0025] The present invention also provides the application of the above composition in the preparation of cosmetics.

[0026] The present invention also provides the application of the composition prepared by the above preparation method in the preparation of cosmetics.

[0027] The present invention also provides the application of the grape seed extract and the Caulerpa taxifolia extract obtained by the above preparation method in a weight ratio of 1:1 - 2 in the preparation of an anti-wrinkle and firming preparation.

[0028] The progressiveness of the present invention compared with the prior art:

[0029] The present invention prepares a composition using active ingredients such as grape seed extract, niacinamide, carnosine, dipotassium glycyrrhizinate, ectoin, Caulerpa taxifolia extract, and ascorbic acid polypeptide, which can effectively improve skin elasticity, has a good anti-wrinkle and firming effect on the skin, and at the same time has high safety and no adverse reactions to the skin.

[0030] The present invention uses a hot water extraction and bromelain extraction method, which endows the grape seed extract with excellent elastase inhibition efficiency and has a good anti-wrinkle and firming effect on the skin.

[0031] The present invention uses a specific strain, Lactobacillus crispatus SHMCC D51652, for fermentation to prepare Caulerpa taxifolia extract, which can effectively improve the variety and richness of active substances, and the extract can effectively resist wrinkles and tighten the skin.

[0032] In the present invention, bromelain is used for enzymatic hydrolysis, which can increase the elastase inhibition rate by about 33%; while the efficiency of inhibiting elastase by grape seed extract obtained by enzymatic hydrolysis with other enzymes is low.

[0033] In the present invention, fermentation with Lactobacillus crispatus SHMCC D51652 can effectively improve the effect of the extract in inhibiting elastase, and the improvement efficiency is about 51%; and compared with other fermentation bacteria, the Lactobacillus crispatus SHMCC D51652 used in the present invention has a higher fermentation efficiency and a better effect of the extract in inhibiting elastase.

[0034] In the present invention, the combination of grape seed extract and Caulerpa taxifolia extract can produce a synergistic effect. Especially when the ratio of the two is in the range of 1:1 - 2, the efficiency of synergistically enhancing the inhibition of elastase activity is higher, and the effect of resisting wrinkles and tightening the skin is better.

[0035] The composition of the present invention is simple to prepare, has excellent effects, and has good application prospects. Detailed Embodiments

[0036] The technical solutions of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all of the embodiments. The description of at least one exemplary embodiment below is actually only illustrative and in no way restrictive of the present invention and its application or use. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.

[0037] Bromelain: S10009, Yuanye Bio-Technology Co., Ltd. Neutral protease: S10013, Yuanye Bio-Technology Co., Ltd. Lactobacillus crispatus SHMCC D51652: purchased from Shanghai Center for Microbial Culture Collection and Preservation. Lactobacillus plantarum: GDMCC NO 1.1516, purchased from Guangdong Microbial Culture Collection Center. Lactobacillus rhamnosus: SHMCC D24725, purchased from Shanghai Center for Microbial Culture Collection and Preservation.

[0038] I. Preparation of Extract

[0039] Grape Seed Extract I

[0040] The preparation method is as follows: Crush Kyoho grape seeds and place them in a reaction vessel. Add water according to the weight ratio of grape seeds to water of 1:30, heat to 55°C, keep warm and stir at 100 rpm for 4 hours, then cool to 25°C to obtain a product. Add bromelain (the amount of bromelain added to the product is 550 u / g of the product) and stir and enzymatically hydrolyze at 45°C and 60 rpm for 3 hours, inactivate the enzyme, filter through a 0.22 μm filter membrane, concentrate the filtrate under reduced pressure to 15.7 wt% of the filtrate, and freeze-dry to a moisture content of 2.24 wt% to obtain grape seed extract I.

[0041] Grape seed extract II

[0042] The preparation method is as follows: Crush Kyoho grape seeds and place them in a reaction vessel. Add water according to the weight ratio of grape seeds to water of 1:20, heat to 57°C, keep warm and stir at 80 rpm for 4.5 hours, then cool to 27°C to obtain a product. Add bromelain (the amount of bromelain added to the product is 500 u / g of the product) and stir and enzymatically hydrolyze at 40°C and 70 rpm for 3.2 hours, inactivate the enzyme, filter through a 0.22 μm filter membrane, concentrate the filtrate under reduced pressure to 15.1 wt% of the filtrate, and freeze-dry to a moisture content of 2.31 wt% to obtain grape seed extract II.

[0043] Grape seed extract III

[0044] The preparation method is as follows: Crush Kyoho grape seeds and place them in a reaction vessel. Add water according to the weight ratio of grape seeds to water of 1:30, heat to 55°C, keep warm and stir at 100 rpm for 4 hours, then cool to 25°C to obtain a product. Stir at 45°C and 60 rpm for 3 hours, filter through a 0.22 μm filter membrane, concentrate the filtrate under reduced pressure to 15.7 wt% of the filtrate, and freeze-dry to a moisture content of 2.24 wt% to obtain grape seed extract III.

[0045] Grape seed extract IV

[0046] The preparation method is as follows: Crush Kyoho grape seeds and place them in a reaction vessel. Add water according to the weight ratio of grape seeds to water of 1:30, heat to 55°C, keep warm and stir at 100 rpm for 4 hours, then cool to 25°C to obtain a product. Add neutral protease (the amount of neutral protease added to the product is 550 u / g of the product) and stir and enzymatically hydrolyze at 45°C and 60 rpm for 3 hours, inactivate the enzyme, filter through a 0.22 μm filter membrane, concentrate the filtrate under reduced pressure to 15.7 wt% of the filtrate, and freeze-dry to a moisture content of 2.24 wt% to obtain grape seed extract IV.

[0047] Grape seed extract V

[0048] The preparation method is as follows: Crush Kyoho grape seeds and place them in a reaction vessel. Add water according to the weight ratio of grape seeds to water of 1:30, heat to 55°C, keep warm and stir at 100 rpm for 4 hours, then cool to 25°C to obtain a product. Add neutral protease and bromelain (the amount of neutral protease added to the product is 225 u / g of the product, and the amount of bromelain is 225 u / g of the product), and stir and enzymatically hydrolyze at 45°C and 60 rpm for 3 hours. Inactivate the enzyme, filter through a 0.22 μm filter membrane, concentrate the filtrate under reduced pressure to 15.7 wt% of the filtrate, and freeze-dry to a moisture content of 2.24 wt% to obtain grape seed extract V.

[0049] Caulerpa taxifolia extract I:

[0050] The preparation method is as follows: Crush Caulerpa taxifolia, add water and glucose according to the weight ratio of Caulerpa taxifolia to water of 1:30, stir at 80 rpm for 5 minutes, sterilize, and then add Lactobacillus crispatus SHMCC D51652 to obtain a material (in the material, the concentration of added glucose is 3.2 wt%, and the concentration of Lactobacillus crispatus SHMCC D51652 is 3.5×10 11 cells / g), then carry out anaerobic fermentation at 30°C for 4 days, sterilize, filter through a 0.22 μm filter membrane, concentrate under reduced pressure to 14.2 wt% of the filtrate weight, and freeze-dry to a moisture content of 1.97 wt% to obtain Caulerpa taxifolia extract I.

[0051] Caulerpa taxifolia extract II:

[0052] The preparation method is as follows: Crush Caulerpa taxifolia, add water and glucose according to the weight ratio of Caulerpa taxifolia to water of 1:35, stir at 70 rpm for 10 minutes, sterilize, and then add Lactobacillus crispatus SHMCC D51652 to obtain a material (in the material, the concentration of added glucose is 2.98 wt%, and the concentration of Lactobacillus crispatus SHMCC D51652 is 4.2×10 11 cells / g), then carry out anaerobic fermentation at 30°C for 3.5 days, sterilize, filter through a 0.22 μm filter membrane, concentrate under reduced pressure to 13.7 wt% of the filtrate weight, and freeze-dry to a moisture content of 2.03 wt% to obtain Caulerpa taxifolia extract II.

[0053] Caulerpa taxifolia extract III:

[0054] The preparation method is as follows: Crush Caulerpa taxifolia, add water according to the weight ratio of Caulerpa taxifolia to water of 1:30, stir at 80 rpm for 5 minutes, sterilize, let stand at 30°C for 4 days, sterilize, filter through a 0.22 μm filter membrane, concentrate under reduced pressure to 14.2 wt% of the filtrate weight, and freeze-dry to a moisture content of 1.97 wt% to obtain Caulerpa taxifolia extract III.

[0055] Caulerpa taxifolia extract IV:

[0056] The preparation method comprises the following steps: crushing the fernleaf algae, adding water and glucose at a weight ratio of 1:30 to the fernleaf algae, stirring at 80 rpm for 5 minutes, sterilizing, and then adding Lactobacillus plantarum (GDMCC NO 1.1516) to obtain a material (the concentration of glucose added in the material is 3.2 wt %, and the concentration of Lactobacillus plantarum is 3.5×10 11 / g), then anaerobically fermented at 30°C for 4 days, sterilized, filtered through a 0.22 μm filter membrane, concentrated under reduced pressure to a filtrate weight of 14.2 wt%, and freeze-dried to a moisture content of 1.97 wt% to obtain Pteris truncatula extract IV.

[0057] Fernleaf Algae Extract V:

[0058] The preparation method is as follows: crush the fernleaf algae, add water and glucose according to the weight ratio of fernleaf algae to water of 1:30, stir at 80 rpm for 5 minutes, sterilize, and then add Lactobacillus rhamnosus (No. SHMCC D24725) to obtain a material (the concentration of glucose added in the material is 3.2wt%, the concentration of Lactobacillus rhamnosus is 3.5×10 11 / g), then anaerobically fermented at 30°C for 4 days, sterilized, filtered through a 0.22 μm filter membrane, concentrated under reduced pressure to a filtrate weight of 14.2 wt%, and freeze-dried to a moisture content of 1.97 wt% to obtain Pteris truncatula extract V.

[0059] 2. Testing

[0060] 1. Extract test

[0061] The elastase inhibition rate was tested to verify the anti-wrinkle and firming effects of the extract. The test method is as follows:

[0062] Buffer: 0.2 mol / L Tris-HCl buffer, pH = 8.0, Shanghai Xuanya Biotechnology Co., Ltd.

[0063] Elastase solution: Use the above buffer as solvent and elastase to prepare a 100 U / mL elastase solution;

[0064] Substrate solution: Use the above buffer as solvent and N-succinyl-alanine-alanine-alanine-p-nitroaniline to prepare a 0.4 mmol / mL substrate solution;

[0065] Sample solution: The above extract was diluted with deionized water to a mass concentration of 2% as the sample solution.

[0066] Prepare the test solutions for the sample group and the sample comparison group corresponding to the sample solution. The sample groups prepared with grape seed extracts I to V are successively the grape seed extract I to V groups; the sample groups prepared with Caulerpa taxifolia extracts I to V are successively the Caulerpa taxifolia extract I to V groups.

[0067] The preparation of the test solutions for each test group is shown in Table 1. Five parallel groups are set up for each group. Add the test solutions of different test groups to a 96-well plate respectively, shake well, incubate at 25 °C for 45 min, and then measure the absorbance OD at 410 nm with a microplate reader.

[0068] According to the calculation formula, elastase inhibition rate / % = 100% × [1 - (OD1 - OD2)] / (OD3 - OD4). Where OD1: OD value of the sample group; OD2: OD value of the sample comparison group; OD3: OD value of the blank group; OD4: OD value of the blank comparison group. The results are shown in Table 2.

[0069] Table 1: Composition of the test solutions for each group

[0070]

[0071] Table 2: Elastase inhibition rates of each group

[0072]

[0073] Combined with the test results in Table 2, it can be seen that the present invention adopts the extraction method of hot water extraction and bromelain, endowing the grape seed extract with excellent efficiency in inhibiting elastase and having a good effect on anti-wrinkle and firming the skin.

[0074] The present invention uses the specific strain Lactobacillus crispatus SHMCC D51652 for fermentation to prepare Caulerpa taxifolia extract, which can effectively improve the variety and abundance of active substances, and the extract can effectively anti-wrinkle and firm the skin.

[0075] According to the tests of grape seed extracts I and III, using bromelain for enzymatic hydrolysis can increase the elastase inhibition rate by about 33%; while the efficiency of inhibiting elastase of grape seed extracts obtained by enzymatic hydrolysis with other enzymes is low, and the above content can be obtained by comparing extracts I with IV and V.

[0076] According to the tests of Caulerpa taxifolia extracts I and III, using Lactobacillus crispatus SHMCC D51652 for fermentation can effectively improve the effect of the extract in inhibiting elastase, and the improvement efficiency is about 51%. And according to the comparison of extracts I with IV and V, compared with other fermentation bacteria, the Lactobacillus crispatus SHMCC D51652 used in the present invention has a higher fermentation efficiency and a better effect of the extract in inhibiting elastase.

[0077] 2. Test of the combination of extracts

[0078] Grape seed extract I and Caulerpa lentillifera extract I were combined and tested for the efficiency of their combination in inhibiting elastase. The test method was the same as that in Test 1 described above; the difference was that the sample solution was prepared by mixing grape seed extract I and Caulerpa lentillifera extract I with deionized water to obtain a total mass concentration of 2% of the extracts as the sample solution. Different sample solutions were prepared by changing the weight ratio of grape seed extract I and Caulerpa lentillifera extract I in the sample solution (while maintaining the total mass concentration of the two in the sample solution at 2%) for testing. The selection of each group ratio and the test results are shown in Table 3.

[0079] Table 3: Elastase inhibition rate

[0080]

[0081] According to the test results in Table 3, it can be seen that the combination of grape seed extract and Caulerpa lentillifera extract can produce a synergistic effect. Especially when the ratio of the two is in the range of 1:1 - 2, the synergistic effect can improve the efficiency of inhibiting elastase activity more, and the effect of anti-wrinkle and firming the skin is better.

[0082] III. Preparation examples and tests

[0083] 1. Preparation examples

[0084] The raw materials and dosages of each composition are shown in Table 4 by weight.

[0085] Table 4: Composition of the composition

[0086]

[0087]

[0088] Note: In Table 4, the grape seed extract used in Compositions 1 and 3 is grape seed extract I, and the grape seed extract used in Composition 2 is grape seed extract II; the Caulerpa lentillifera extract used in Compositions 1 and 2 is Caulerpa lentillifera extract I, and the Caulerpa lentillifera extract used in Composition 3 is Caulerpa lentillifera extract II.

[0089] Nicotinamide: Fante Rust Special Chemicals (Nantong) Co., Ltd. Carnosine: RF - 291, Guangzhou Tongjuan Pharmaceutical Technology Co., Ltd. Dipotassium glycyrrhizinate: RL - 118, Gansu Fanzhi Pharmaceutical Co., Ltd. Ectoine: RF - 159, Nanjing Xianda Pharmaceutical Technology Co., Ltd. Ascorbyl polypeptide: RP - 044, Guangzhou Nuoyan Biotechnology Co., Ltd.

[0090] The preparation method of the above composition is as follows: Add each raw material into a reaction kettle, heat to 50°C, stir at 75 rpm for 0.5 hours, let it stand and age at room temperature for 1 hour, sterilize, and fill to obtain the composition.

[0091] 2. Testing

[0092] Test 1: Conduct a safety test on the above composition. The test process is as follows: Select healthy male volunteers aged 30 - 50 years old, randomly divide them into 3 groups, with 10 people in each group. Clean the outer side of the right forearm of the volunteers with clean water, dry it with a cotton cloth, and use a patch tester to conduct a closed patch test to test safety; Take 0.025 g of each of Compositions 1 - 3 and apply them on the patch tester and stick it on the test area. Remove the patch tester after 24 hours, and evaluate the skin of each volunteer according to the criteria in Table 5 after 60 minutes. The results are shown in Table 6.

[0093] Table 5: Criteria

[0094]

[0095] Table 6: Patch test

[0096]

[0097] Combined with the test results in Table 6, the composition of the present invention has low irritation, no reaction to the skin, and high safety.

[0098] Test 2: Conduct a test on increasing skin elasticity for Compositions 1 - 3 above. The test process is as follows: Select 12 healthy female volunteers with good skin, and randomly assign them to 3 groups according to age, with 4 people in each group for testing.

[0099] Application site: Face; Application method: After cleansing the face in the morning and evening, drop the product on the face and spread it evenly; Dosage: 1.2 g each time; Application time: Continuously apply for 45 days.

[0100] Testing instrument: Cutometer, MPA580; Testing index: Parameter R2 (the ratio of the rebound amount without negative pressure to the maximum stretching amount with negative pressure), the closer the value is to 1, the better the skin elasticity.

[0101] Test and calculate the average value of the skin elasticity parameter R2 of the cheeks of each group of volunteers before use and after 45 days of use, and calculate the increase value △R2 of R2 after 30 days of use compared with before use; The results are shown in Table 7.

[0102] Table 7: Skin elasticity parameters

[0103]

[0104] Combined with the test in Table 7, it can be seen that the composition of the present invention can effectively improve skin elasticity and has a good effect of anti-wrinkle and firming the skin.

[0105] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art can still modify the technical solutions described in the foregoing embodiments, or perform equivalent replacements on some of the technical features; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the spirit and scope of the technical solutions required to be protected by the present invention.

Claims

1. An anti-wrinkle and firming composition, characterized in that, The raw material composition is: grape seed extract, niacinamide, carnosine, dipotassium glycyrrhizinate, ectoine, Caulerpa lentillifera extract, ascorbyl polypeptide.

2. The composition according to claim 1, characterized in that, By weight, the raw material composition is: 20-40 parts of grape seed extract, 10-30 parts of niacinamide, 10-30 parts of carnosine, 5-15 parts of dipotassium glycyrrhizinate, 10-30 parts of ectoine, 20-40 parts of Caulerpa lentillifera extract, 10-20 parts of ascorbyl polypeptide.

3. The composition according to claim 1 or 2, characterized in that, By weight, the raw material composition also contains 50-500 parts of polyol and 200-3000 parts of water.

4. The composition according to claim 3, wherein The polyol includes at least one of ethylene glycol, propylene glycol, butylene glycol, pentylene glycol, hexylene glycol, glycerol, polyethylene glycol, octylene glycol.

5. A method for preparing the composition according to any one of claims 1-4, characterized in that, The preparation method includes: heating and stirring the raw materials, standing for aging, sterilizing and filling.

6. The preparation method according to claim 5, wherein The preparation method of grape seed extract includes: crushing grape seeds, adding water and heating and stirring, then cooling to obtain a product, adding bromelain for enzymolysis, inactivating the enzyme, filtering, concentrating and drying to obtain grape seed extract.

7. The preparation method according to claim 5, characterized in that The preparation method of Caulerpa lentillifera extract includes: pulverizing Caulerpa lentillifera, adding water and glucose and stirring, sterilizing, then adding Lactobacillus crispatus SHMCC D51652 to obtain a material, then performing anaerobic fermentation, sterilizing, filtering, concentrating and drying to obtain Caulerpa lentillifera extract.

8. Use of the composition according to any one of claims 1-4 in the preparation of cosmetics.

9. Use of the composition prepared by the preparation method according to any one of claims 5-7 in the preparation of cosmetics.

10. Use of grape seed extract and Caulerpa lentillifera extract in a weight ratio of 1:1-2 in the preparation of an anti-wrinkle and firming preparation.

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