Ganoderma lucidum spore oil composition helpful for enhancing immunity and preparation method thereof

Abstract

The invention belongs to the technical field of biology, and particularly relates to a ganoderma lucidum spore oil composition helpful for enhancing immunity and a preparation method of the ganoderma lucidum spore oil composition. The ganoderma lucidum spore oil composition comprises the following raw materials in parts by weight: 75-85 parts of ganoderma lucidum spore oil, 4-8 parts of codonopsis pilosula polysaccharide, 5-10 parts of capillarisin, 2-5 parts of lecithin and 5-8 parts of a stabilizer. According to the ganoderma lucidum spore oil composition, the oxidation resistance of ganoderma lucidum spore oil can be improved, the long-acting stability of the composition can be maintained, and then the ganoderma lucidum spore oil composition can play a role in enhancing immunity.

Description

Technical Field

[0001] The present invention belongs to the field of biotechnology, and particularly relates to a Ganoderma lucidum spore oil composition helpful for enhancing immunity and a preparation method thereof. Background Art

[0002] As a key node in the continuum between clinical health and disease, the pathological characteristics of the sub-healthy state are mainly manifested as chronic fatigue syndrome (CFS), a significant decline in the body vitality index (VPI), and an imbalance in the immune system homeostasis. According to epidemiological surveys, the proportion of sub-healthy people globally has exceeded 75%, and those with abnormal immune function disorder-related indicators account for 92.3%. This intermediate state between health and disease has posed a major public health challenge in modern society. Based on the medical concept of "preventive treatment of disease", intervening through immunomodulation to block the evolution of sub-health to a pathological state has important strategic value for disease prevention. Currently, with the continuous in-depth study of immunology and the continuous increase in the number of sub-healthy people, the demand for drugs that can improve immune function is becoming increasingly urgent.

[0003] Ganoderma lucidum spore oil is a fat-soluble bioactive complex extracted from mature Ganoderma lucidum spores by supercritical CO2 fluid extraction technology, mainly containing triterpenoids (ganoderic acids A-G), sterols (ergosterol), unsaturated fatty acids (ω-6 / ω-3 polyunsaturated fatty acids), and immune polysaccharides (β-1,3-glucan) and other active ingredients. Pharmacological studies have shown that this extract can enhance macrophage phagocytic activity by activating the NF-κB signaling pathway, and its immune-enhancing effect shows a dose-dependent relationship.

[0004] Although Ganoderma lucidum spore oil has significant medicinal potential, there are still three major technical bottlenecks in its physical form: 1) the double bonds of unsaturated fatty acids are easily oxidized; 2) the stability of the liquid lipid system is poor; 3) the bioavailability for people with insufficient bile secretion is insufficient. These problems seriously restrict its clinical application efficacy. Therefore, developing a new type of Ganoderma lucidum spore oil preparation with strong oxidation stability, high absorption efficiency, and long-lasting action has become a technical bottleneck that urgently needs to be broken through in the field of immunomodulatory preparations. Summary of the Invention

[0005] In order to overcome the deficiencies of the prior art, the first object of the present invention is to provide a Ganoderma lucidum spore oil composition helpful for enhancing immunity, which can improve the antioxidant capacity of Ganoderma lucidum spore oil, maintain the long-term stability of the composition, and thus help it exert its ability to enhance immunity.

[0006] The second object of the present invention is to provide a preparation method of a Ganoderma lucidum spore oil composition helpful for enhancing immunity.

[0007] In order to achieve the above object, the technical solution adopted by the present invention is:

[0008] A Ganoderma lucidum spore oil composition helpful for enhancing immunity, the composition comprising the following raw materials in parts by weight: 75-85 parts of Ganoderma lucidum spore oil, 4-8 parts of codonopsis pilosula polysaccharide, 5-10 parts of capillarisin, 2-5 parts of lecithin, and 5-8 parts of stabilizer.

[0009] Further, the composition comprises the following raw materials in parts by weight: 80 parts of Ganoderma lucidum spore oil, 7 parts of codonopsis pilosula polysaccharide, 8 parts of capillarisin, 3 parts of lecithin, and 7 parts of stabilizer.

[0010] Further, the preparation method of the stabilizer comprises the following steps:

[0011] (1) Adding dopamine hydrochloride into Tris-HCl buffer solution to obtain a mixed solution; adding nano-selenium particles into the mixed solution for reaction, centrifuging, collecting the solid matter and washing to obtain nano-selenium particles coated with polydopamine;

[0012] (2) Dispersing the nano-selenium particles coated with polydopamine in water, then adding rosmarinic acid, adjusting the pH and stirring, centrifuging, washing and drying to obtain the stabilizer.

[0013] Further, in step (1), the concentration of dopamine hydrochloride in the mixed solution is 0.8-1.2 mg / mL; the pH of the Tris-HCl buffer solution is 8.0-9.0.

[0014] Further, in step (1), the mass ratio of the nano-selenium particles to dopamine hydrochloride is (1-2):1; the reaction time is 8-12 h.

[0015] Further, in step (2), the mass ratio of the nano-selenium particles coated with polydopamine to rosmarinic acid is (3-5):1; the value of the pH is 2-3; the stirring time is 4-5 h.

[0016] Further, the dosage form of the composition is soft capsule; the components of the capsule skin of the soft capsule include gelatin, glycerol and water.

[0017] The preparation method of the above Ganoderma lucidum spore oil composition helpful for enhancing immunity comprises the following steps:

[0018] (a) Mixing and shearing Ganoderma lucidum spore oil, codonopsis pilosula polysaccharide, capillarisin, lecithin and stabilizer according to the proportion to obtain the core material liquid;

[0019] (b) Mixing water and glycerol and heating to 65-75 °C, adding gelatin, stirring and evacuating under sealed conditions, and then standing to obtain the capsule skin glue liquid;

[0020] (c) Inject the core material liquid into the capsule skin made of the capsule skin glue solution under the environmental conditions of 18-26°C. After processes such as pressing, shaping, pill washing, and drying, a ganoderma spore oil composition is obtained.

[0021] Furthermore, the mass ratio of water, glycerol, and gelatin in step (b) is 100:(45-50):(110-115).

[0022] Furthermore, the filling amount of the core material liquid in step (c) is 0.3-0.6 g / granule; the temperature of shaping is 20-25°C; the mass ratio of the core material liquid to the capsule skin glue solution is 100:(27-53).

[0023] The beneficial technical effects of the present invention are as follows:

[0024] 1. The present invention provides a ganoderma spore oil composition that helps to enhance immunity, containing components such as ganoderma spore oil and a stabilizer. The stabilizer is prepared by coating polydopamine and the antioxidant rosmarinic acid on nano-selenium particles. Nano-selenium itself has excellent antioxidant ability, and rosmarinic acid, as a powerful antioxidant, forms a stable complex with dopamine. Adding it as a stabilizer to the composition can improve the antioxidant performance of ganoderma spore oil, maintain its long-term stability, help it play the role of enhancing immunity, and can also supplement the essential selenium element for the human body, further enhancing its health benefits; in addition, the present invention also combines codonopsis pilosula polysaccharide and capillarisinone with ganoderma spore oil, which can further enhance immunity and effectively promote bile secretion, thereby accelerating the absorption of ganoderma spore oil by the human body and further enhancing the medicinal effect of ganoderma spore oil.

[0025] 2. The present invention also provides a preparation method of the above-mentioned ganoderma spore oil composition that helps to enhance immunity. The raw materials used in the preparation method are cheap, and the preparation process is simple, which is beneficial for large-scale production. Detailed Embodiments

[0026] The following content is a further detailed description of the present invention in combination with specific preferred embodiments. It cannot be determined that the specific implementation of the present invention is only limited to these descriptions. For those of ordinary skill in the technical field to which the present invention belongs, without departing from the concept of the present invention, several simple deductions or substitutions can be made, which should all be regarded as belonging to the protection scope of the present invention. The specific conditions not indicated in the examples are carried out according to the conventional conditions or the conditions recommended by the manufacturer. The reagents or instruments used, unless otherwise specified, are all conventional products obtained through commercial channels.

[0027] (I) Examples

[0028] Example 1

[0029] Example 1 provides a Ganoderma lucidum spore oil composition that helps enhance immunity, comprising the following raw materials in parts by weight: 80 parts of Ganoderma lucidum spore oil, 6 parts of codonopsis pilosula polysaccharide, 8 parts of capillarisin, 3 parts of lecithin, and 7 parts of stabilizer.

[0030] The preparation method of the stabilizer comprises the following steps:

[0031] (1) Add dopamine hydrochloride to a Tris-HCl buffer solution with a pH of 8.5 to obtain a mixed solution with a dopamine hydrochloride concentration of 1 mg / mL. Then, add nano-selenium particles with a particle size of 120 nm to the mixed solution, and perform ultrasonic dispersion to make the nano-selenium particles evenly distributed. The mass ratio of nano-selenium particles to dopamine hydrochloride is 1.5:1. After reacting for 10 h, perform centrifugal separation on the reaction solution, collect the solid matter and wash it to obtain nano-selenium particles coated with polydopamine;

[0032] (2) According to the mass ratio of nano-selenium particles coated with polydopamine to rosmarinic acid of 4:1, add nano-selenium particles coated with polydopamine and rosmarinic acid to water, then adjust the pH to 2.5 and stir for 5 h to promote the adsorption of rosmarinic acid by dopamine. After stirring is completed, perform centrifugation, washing, and drying to obtain the stabilizer.

[0033] Example 1 also provides a preparation method of the above Ganoderma lucidum spore oil composition that helps enhance immunity, comprising the following steps:

[0034] (a) Mix the Ganoderma lucidum spore oil, codonopsis pilosula polysaccharide, capillarisin, lecithin, and stabilizer evenly according to the above parts by weight, and perform high-speed shearing at 3000 rpm for 8 min to obtain the core material liquid;

[0035] (b) Mix water and glycerol evenly and heat to 70 °C, then add gelatin, and stir evenly under sealed conditions, evacuate to remove air bubbles, and then let it stand to obtain the capsule skin glue liquid; the mass ratio of water, glycerol, and gelatin is 25:12:28;

[0036] (c) Quantitatively pour 0.4 g / granule of the core material liquid into the capsule skin made from the capsule skin glue liquid at 24 °C, then press and combine the pills and shape them at 23 °C. After processes such as pill washing and drying, the Ganoderma lucidum spore oil composition is prepared, and the mass ratio of the core material liquid to the capsule skin glue liquid is 5:2.

[0037] Example 2

[0038] Example 2 provides a Ganoderma lucidum spore oil composition that helps enhance immunity, comprising the following raw materials in parts by weight: 75 parts of Ganoderma lucidum spore oil, 4 parts of codonopsis pilosula polysaccharide, 5 parts of capillarisin, 2 parts of lecithin, and 5 parts of stabilizer.

[0039] The preparation method of the stabilizer comprises the following steps:

[0040] (1) Add dopamine hydrochloride to Tris-HCl buffer solution with pH = 8 to obtain a mixed solution with a concentration of dopamine hydrochloride of 0.8 mg / mL. Then, add selenium nanoparticles with a particle size of 100 nm to the mixed solution, and ultrasonically disperse to make the selenium nanoparticles evenly distributed. The mass ratio of selenium nanoparticles to dopamine hydrochloride is 1:1. After reacting for 8 h, centrifuge the reaction solution, collect the solid matter and wash it to obtain selenium nanoparticles coated with polydopamine;

[0041] (2) According to the mass ratio of selenium nanoparticles coated with polydopamine to rosmarinic acid of 3:1, add selenium nanoparticles coated with polydopamine and rosmarinic acid to water, and then adjust the pH to 2 and stir for 4 h to promote the adsorption of rosmarinic acid by dopamine. After stirring, centrifuge, wash, and dry to obtain a stabilizer.

[0042] Example 2 also provides a preparation method of the above-mentioned ganoderma spore oil composition helpful for enhancing immunity, including the following steps:

[0043] (a) Mix ganoderma spore oil, codonopsis pilosula polysaccharide, capillarisin, lecithin, and stabilizer evenly according to the above weight parts, and shear at a high speed of 4000 rpm for 5 min to obtain a core material liquid;

[0044] (b) Mix water and glycerol evenly and heat to 65 °C, then add gelatin, and stir evenly under sealed conditions, evacuate to remove air bubbles, and then let it stand to obtain a capsule skin glue liquid; the mass ratio of water, glycerol, and gelatin is 20:9:22;

[0045] (c) Quantitatively pour 0.3 g / granule of the core material liquid into the capsule skin made of the capsule skin glue liquid at 18 °C, then press and combine into pills and shape at 20 °C, and obtain the ganoderma spore oil composition after processes such as pill washing and drying, where the mass ratio of the core material liquid to the capsule skin glue liquid is 100:53.

[0046] Example 3

[0047] Example 3 provides a ganoderma spore oil composition helpful for enhancing immunity, including the following raw materials in parts by weight: 85 parts of ganoderma spore oil, 8 parts of codonopsis pilosula polysaccharide, 10 parts of capillarisin, 5 parts of lecithin, and 8 parts of stabilizer.

[0048] The preparation method of the stabilizer includes the following steps:

[0049] (1) Add dopamine hydrochloride to a Tris-HCl buffer solution with a pH of 9 to obtain a mixed solution with a dopamine hydrochloride concentration of 1.2 mg / mL. Then, add selenium nanoparticles with a particle size of 150 nm to the mixed solution and ultrasonically disperse them to make the selenium nanoparticles evenly distributed. The mass ratio of selenium nanoparticles to dopamine hydrochloride is 2:1. After reacting for 12 h, centrifuge the reaction solution, collect the solid matter and wash it to obtain selenium nanoparticles coated with polydopamine;

[0050] (2) According to the mass ratio of 5:1 of polydopamine-coated selenium nanoparticles to rosmarinic acid, add polydopamine-coated selenium nanoparticles and rosmarinic acid to water, then adjust the pH to 3 and stir for 5 h to promote the adsorption of rosmarinic acid by dopamine. After stirring, centrifuge, wash, and dry to obtain a stabilizer.

[0051] Example 3 also provides a preparation method of the above-mentioned ganoderma spore oil composition that helps enhance immunity, including the following steps:

[0052] (a) Mix the ganoderma spore oil, codonopsis pilosula polysaccharide, capillarisin, lecithin, and stabilizer evenly according to the above weight parts, and shear at a high speed of 5000 rpm for 8 min to obtain a core material liquid;

[0053] (b) Mix water and glycerol evenly and heat to 75 °C, then add gelatin and stir evenly under sealed conditions, evacuate to remove air bubbles, and then let it stand to obtain a capsule skin glue liquid; the mass ratio of water, glycerol, and gelatin is 20:10:23;

[0054] (c) Quantitatively pour 0.6 g / granule of the core material liquid into the capsule skin made of the capsule skin glue liquid at 26 °C, then press and combine the pills and shape them at 25 °C. After processes such as pill washing and drying, the ganoderma spore oil composition is prepared, and the mass ratio of the core material liquid to the capsule skin glue liquid is 100:27.

[0055] (II) Comparative Examples

[0056] Comparative Example 1

[0057] Comparative Example 1 is basically the same as Example 1, and the difference is that: the stabilizer in Example 1 is replaced with an equal amount of selenium nanoparticles.

[0058] Comparative Example 2

[0059] Comparative Example 2 is basically the same as Example 1, and the difference is that: step (2) in the preparation method of the stabilizer in Example 1 is omitted.

[0060] Comparative Example 3

[0061] Comparative Example 3 is basically the same as Example 1, except that: the stabilizer in Example 1 is replaced with a mixture of nano-selenium particles and rosmarinic acid, and the dosages of nano-selenium particles and rosmarinic acid are the same as those in Example 1.

[0062] Comparative Example 4

[0063] Comparative Example 4 is basically the same as Example 1, except that: cirsimaritin in Example 1 is not added.

[0064] (III) Test Examples

[0065] Test Example 1

[0066] An accelerated stability test was conducted on the Ganoderma lucidum spore oil compositions prepared in each group of Example 1 and Comparative Examples 1-4. The specific method was as follows: The Ganoderma lucidum spore oil compositions prepared in each group were sealed in self-sealing bags and placed in a stability test chamber at 37 °C and 75% relative humidity. The peroxide values of each group were detected according to GB 5009.227-2023 "Determination of peroxide value in foods" at the 0th, 1st, and 3rd months respectively. The results are shown in Table 1.

[0067] Table 1 Product oxidation stability experiment

[0068]

[0069] As can be seen from Table 1, the peroxide value of the composition obtained in Example 1 was significantly lower than that of Comparative Examples 1-4, indicating that the Ganoderma lucidum spore oil composition of the present invention has excellent stability.

[0070] In Comparative Example 1, the stabilizer was replaced with nano-selenium particles. In Comparative Example 2, only polydopamine was coated on the surface of nano-selenium. In Comparative Example 3, nano-selenium and rosmarinic acid were simply mixed. The peroxide values of Comparative Examples 1-3 were all significantly higher than that of Example 1, indicating that the stabilizer of the present invention can effectively improve the stability of Ganoderma lucidum spore oil. Analyzing the reasons, it can be known that: the stabilizer is composed of rosmarinic acid and nano-selenium particles coated with dopamine on the surface. Nano-selenium itself has excellent antioxidant ability, and rosmarinic acid, as a powerful antioxidant, forms a stable complex with dopamine. Adding it as a stabilizer to the composition can significantly improve the antioxidant performance of Ganoderma lucidum spore oil and maintain the long-term stability of Ganoderma lucidum spore oil.

[0071] Test Example 2

[0072] The effects of enhancing immunity of the samples prepared in Example 1 and Comparative Examples 1-4 were tested.

[0073] 1. Experimental animals, grouping, and dosing regimens:

[0074] 160 SPF - level male Kunming mice, weighing 18 - 22 g, were selected and divided into Immunization Group I and Immunization Group II. Immunization Group I was used to measure the NK cell activity, and Immunization Group II was used to measure the number of antibody - producing cells. Both Immunization Group I and Immunization Group II were set up with low, medium, and high - dose groups of Example 1, groups of Comparative Example 1 - 4, and a blank control group, with 10 mice in each group.

[0075] The dosing doses of the low, medium, and high - dose groups of Example 1 were 0.20, 0.40, and 1.20 g / kg / day respectively (equivalent to 5, 10, and 30 times the recommended human dosage), the dosing dose of the groups of Comparative Example 1 - 4 was 1.20 g / kg / day, and the blank control group was given an equal amount of water. It was administered orally once a day. The gavage volume for the mice was 0.1 mL / 10 g body weight. After continuous gavage for 33 days, various immune indexes were measured.

[0076] 2. Experimental environmental conditions:

[0077] The experimental conditions were a barrier environment, with a temperature of 22 - 24 °C and a humidity of 52 - 58%.

[0078] 3. Experimental methods

[0079] 3.1 Measurement of NK cell activity (lactate dehydrogenase assay)

[0080] After the administration was completed, the mice in each group were sacrificed by cervical dislocation. The spleen was aseptically removed and placed in a small petri dish containing an appropriate amount of Hank's solution. The spleen was ground to make a single - cell suspension, filtered through a 200 - mesh sieve, washed twice with Hank's solution, centrifuged at 1000 r / min for 10 min each time, the supernatant was discarded, the cell mass was resuspended, 0.5 mL of sterile water was added for 20 seconds, centrifuged and the supernatant was discarded, red blood cell lysate was added. After lysing the red blood cells, 0.5 mL of 2 - fold Hank's solution and 8 mL of Hank's solution were added, centrifuged at 1000 rpm for 10 min, and resuspended with 1 mL of RPMI 1640 complete culture medium containing 10% calf serum. The cell concentration was adjusted by trypan blue staining and counting (the number of viable cells should be above 95%). The cell concentration was adjusted to 2×10 7 cells / mL, which were the effector cells; YAC - 1 cells with good growth 24 h after passage were taken, and the cell concentration was adjusted to 4×10 5cells / mL, and these are the target cells. Take 100 μL each of the target cells and effector cells (effector-to-target ratio of 50:1), and add them to a U-shaped 96-well culture plate; add 100 μL each of the target cells and culture medium to the natural release well of the target cells, and add 100 μL each of the target cells and 2.5% Triton to the maximum release well of the target cells; set three parallel wells for each of the above items, culture in an incubator at 37 °C and 5% CO2 for 4 h, then centrifuge the 96-well culture plate at 1500 r / min for 5 min, aspirate 100 μL of the supernatant from each well and place it in a flat-bottom 96-well culture plate, simultaneously add 100 μL of the LDH substrate solution, react for 5 min according to room temperature, add 30 μL of 1 mol / L HCl to each well, measure the optical density value (OD) at 490 nm with an enzyme-linked immunosorbent assay (ELISA) reader, and calculate the NK cell activity = [(OD of the reaction well - OD of the natural release well) / (OD of the maximum release well - OD of the natural release well)] × 100%, and the results are shown in Table 2.

[0081] 3.2 Detection of antibody-producing cells (Jerne modified slide method)

[0082] Prepare packed SRBC from sheep blood, and prepare a 2% (v / v) cell suspension of the packed SRBC with normal saline. On the 28th day after administration to each group of mice in Step 1, inject 0.2 mL intraperitoneally into each mouse for immunization. After 5 days, sacrifice the mice, take the spleen, gently grind it, make a cell suspension with Hank's solution, filter it through a 200-mesh sieve, wash and centrifuge twice, and finally suspend the cells in 8 mL of Hank's solution. Count the cells and adjust the cell concentration to 5×10 6 cells / mL. After heating and dissolving the surface medium (1 g of agarose added to double-distilled water to make 100 mL), mix it with an equal volume of 2-fold concentrated Hank's solution (pH 7.4), dispense it into small test tubes, 0.5 mL per tube, and then add 50 μL of 10% (v / v) SRBC prepared with SA buffer and 20 μL of the spleen cell suspension (5×10 6 cells / mL) to each tube. After quickly mixing evenly, pour it onto a slide coated with a thin layer of agarose. After the agarose solidifies, place the slide flat on a slide rack and incubate it in a carbon dioxide incubator for 1.5 h. Add guinea pig serum complement diluted with SA buffer (volume ratio of SA buffer to guinea pig serum is 8:1) to the groove of the slide rack, continue to incubate for 1.5 h, and then count the number of hemolytic plaques. The results are shown in Table 3.

[0083] Table 2 Tests of the NK cell activity of mice in the blank control group and each example

[0084]

[0085]

[0086] Table 3 Determination of antibody-producing cells of mice in the blank control group and each example

[0087] Grouping Dose (g / kg) Number of animals (n) <![CDATA[Number of hemolytic plaques (number / 10 6 spleen cells)]]> Blank control group 0 10 104±42 Low-dose group of Example 1 0.2 10 132±37 Medium-dose group of Example 1 0.4 10 141±50 High-dose group of Example 1 1.2 10 165±36 Group of Comparative Example 1 1.2 10 119±41 Group of Comparative Example 2 1.2 10 126±34 Group of Comparative Example 3 1.2 10 128±36 Group of Comparative Example 4 1.2 10 121±38

[0088] As can be seen from Tables 2 to 3, compared with the blank control group and Comparative Examples 1-4, the NK cell activity and antibody-producing cells in the Example 1 group were significantly improved. Moreover, it was found that as the dose increased, the NK cell activity and antibody-producing cells in mice were also significantly improved, indicating that the Ganoderma lucidum spore oil composition fully exerted its function of enhancing immunity.

[0089] Compared with the Example 1 group, in the Comparative Example 1-3 groups, the preparation process of the stabilizer was adjusted, and the immune effect decreased significantly because the stability of the Ganoderma lucidum spore oil was affected, thereby affecting its immune ability. In Comparative Example 4, capillarisin was omitted, and the immune effect also decreased. This indicates that the combination of capillarisin and Ganoderma lucidum spore oil can further enhance immunity while effectively promoting bile secretion, thereby accelerating the absorption of Ganoderma lucidum spore oil by the human body and further enhancing the efficacy of Ganoderma lucidum spore oil.

[0090] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. The basic principles and main features of the present invention have been described with specific implementation schemes above. On the basis of the present invention, some modifications or replacements can be made, but these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the scope of protection required by the present invention.

Claims

1. A ganoderma spore oil composition that helps enhance immunity, characterized in that, The composition comprises the following raw materials in parts by weight: 75-85 parts of ganoderma spore oil, 4-8 parts of codonopsis pilosula polysaccharide, 5-10 parts of capillarisin, 2-5 parts of lecithin, and 5-8 parts of stabilizer.

2. The Ganoderma lucidum spore oil composition according to claim 1, which is helpful for enhancing immunity, is characterized in that, The composition comprises the following raw materials in parts by weight: 80 parts of ganoderma spore oil, 7 parts of codonopsis pilosula polysaccharide, 8 parts of capillarisin, 3 parts of lecithin, and 7 parts of stabilizer.

3. The Ganoderma lucidum spore oil composition according to claim 1, which is helpful for enhancing immunity, is characterized in that, The preparation method of the stabilizer comprises the following steps: (1) Add dopamine hydrochloride into Tris-HCl buffer solution to obtain a mixed solution; add nano-selenium particles into the mixed solution for reaction, centrifuge, collect the solid and wash it to obtain nano-selenium particles coated with polydopamine; (2) Disperse the nano-selenium particles coated with polydopamine in water, then add rosmarinic acid, adjust the pH and stir, centrifuge, wash and dry to obtain the stabilizer.

4. The ganoderma spore oil composition according to claim 3, which is helpful for enhancing immunity, is characterized in that, In step (1), the concentration of dopamine hydrochloride in the mixed solution is 0.8-1.2 mg / mL; the pH of the Tris-HCl buffer solution is 8.0-9.

0.

5. The Ganoderma lucidum spore oil composition according to claim 3, which is helpful for enhancing immunity, is characterized in that, In step (1), the mass ratio of the nano-selenium particles to dopamine hydrochloride is (1-2):1; the reaction time is 8-12 h.

6. The Ganoderma lucidum spore oil composition according to claim 3, which is helpful for enhancing immunity, is characterized in that, In step (2), the mass ratio of the nano-selenium particles coated with polydopamine to rosmarinic acid is (3-5):1; the pH value is 2-3; the stirring time is 4-5 h.

7. The ganoderma spore oil composition according to claim 1, which is helpful for enhancing immunity, is characterized in that The dosage form of the composition is soft capsule; the components of the capsule skin of the soft capsule include gelatin, glycerol and water.

8. The preparation method of the ganoderma spore oil composition according to claim 7, which is helpful for enhancing immunity, is characterized in that, Comprises the following steps: (a) Mix and shear ganoderma spore oil, codonopsis pilosula polysaccharide, capillarisin, lecithin and stabilizer in proportion to obtain the core material liquid; (b) Mix water and glycerol and heat to 65-75 °C, add gelatin, stir and evacuate under sealed conditions, and then stand still to obtain the capsule skin glue liquid; (c) Inject the core material liquid into the capsule skin made of the capsule skin glue liquid under the environmental condition of 18-26 °C, and obtain the ganoderma spore oil composition after pressing, shaping, pill washing and drying processes.

9. The preparation method of the ganoderma spore oil composition according to claim 8, which is helpful for enhancing immunity, is characterized in that, In step (b), the mass ratio of water, glycerol and gelatin is 100:(45-50):(110-115).

10. The preparation method of the ganoderma spore oil composition according to claim 8, which helps to enhance immunity, is characterized in that, In step (c), the filling amount of the core material liquid is 0.3-0.6 g / granule; the shaping temperature is 20-25 °C; the mass ratio of the core material liquid to the capsule skin glue liquid is 100:(27-53).

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