Bacteriostatic agent for pets and preparation method thereof
By preparing pet antibacterial agents containing streptococcin, ε-polylysine hydrochloride and natamycin, the problem of poor effectiveness of existing antibacterial agents is solved, broad-spectrum inhibition of pet bacteria and fungi and wound healing promotion are achieved, and safe and irritating the pet skin.
Patent Information
- Application Number
- CN202510523455.X
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-04-24
- Publication Date
- 2025-08-01
AI Technical Summary
The antibacterial properties of existing pet antibacterial agents are not ideal, they are very irritating to pet skin, and have a single treatment direction, which is inconvenient to operate, so they cannot fully prevent and treat pet bacteria and fungal infections.
Streptococcus lactate as the main component, supplemented with ε-polylysine hydrochloride, natamycin and trehalose, to adjust the pH value, and prepare a bacterial inhibitor for pets, which is dissolved by stirring and filtered to obtain a bacterial inhibitor.
It achieves broad-spectrum inhibition of bacteria and fungi, promotes wound healing, has significant effects, quick results, no obvious irritation to pet skin, and is easy to use.
Smart Images
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Abstract
Description
Technical Field
[0001] The present invention relates to the technical field of prevention and treatment of pet bacterial and fungal infections, and specifically relates to an antibacterial agent for pets and a preparation method thereof. Background Art
[0002] Pet bacterial and fungal infections are a major challenge in the current field of pet health. With the increasing status of pets in families, the technical need for preventing and treating these infections is becoming increasingly urgent. Pet antibacterial agents are a type of pet health care product designed specifically for pets. Its main functions are to kill and inhibit various bacteria, viruses, and fungi on pets, prevent the occurrence of infections and contagious diseases, and promote wound healing. There are mainly the following three treatment directions: 1. Interdigital dermatitis: It is a type of claw dermatitis mainly caused by bacteria (Staphylococcus aureus, Staphylococcus intermedius, Streptococcus, Escherichia coli), fungi (Candida albicans), and mites. The antibacterial agent for pets contains antibacterial components, which can effectively inhibit the growth of bacteria and block their reproduction and spread. 2. Trauma, infected wounds, and fresh wounds: The pet antibacterial agent can clean the skin surface of pets, remove dirt and odors, and has a certain disinfection effect. It can kill germs and viruses on the pet's body surface, reduce the risk of infection, promote wound healing, and relieve the pain of pets. 3. Postoperative wounds: The main function of the pet antibacterial agent is to prevent and control wound infections and promote wound healing. Such products usually contain antibacterial components, which can effectively kill or inhibit bacteria on the wound surface and reduce the risk of postoperative complications. However, the antibacterial performance of existing pet disinfectants on the market is not ideal at present, and they also cause relatively large irritation to the skin of pets. Many pet skin infection prevention and treatment products have a single treatment direction and incomplete effects, requiring multiple medications, which is very inconvenient to operate.
[0003] In order to overcome the defects of the prior art, the present invention provides an antibacterial agent for pets and a preparation method thereof, which have a certain therapeutic and healing effect on preventing wound infections such as interdigital dermatitis, trauma, infected wounds, and fresh wounds. Summary of the Invention
[0004] The purpose of the present invention is to provide an antibacterial agent for pets and a preparation method thereof to solve the problems in the prior art.
[0005] To solve the above technical problems, the present invention provides the following technical solutions:
[0006] An antibacterial agent for pets contains nisin with an effective price of 500 - 2000 IU / mL.
[0007] Preferably, in terms of mass percentage, the auxiliary materials of the antibacterial agent specifically include: 0.01 - 0.1% ε-polylysine hydrochloride, 0.02 - 0.2% natamycin, 2 - 8% trehalose, and an appropriate amount of glacial acetic acid is used to adjust the pH value, and the balance is water.
[0008] Preferably, it contains nisin with an effective titer of 500 - 1500 IU / mL.
[0009] Preferably, in terms of mass percentage, the excipients of the bacteriostatic agent specifically include: 0.02 - 0.05% ε - polylysine hydrochloride, 0.02 - 0.1% natamycin, 2 - 5% trehalose, and an appropriate amount of glacial acetic acid is used to adjust the pH value, and the balance is water.
[0010] A preparation method of a bacteriostatic agent for pets includes the following steps: successively adding nisin, ε - polylysine hydrochloride, natamycin, and trehalose into water, stirring and dissolving, then adding an appropriate amount of glacial acetic acid to adjust the pH to 3.0 - 5.0, and filtering to obtain the bacteriostatic agent.
[0011] Preferably, it includes the following steps: successively adding nisin with an effective titer of 500 - 1500 IU / mL, 0.02 - 0.05% ε - polylysine hydrochloride, 0.02 - 0.1% natamycin, and 2 - 5% trehalose into water, stirring and dissolving, then adding an appropriate amount of glacial acetic acid to adjust the pH to 3.0 - 5.0, and filtering to obtain the bacteriostatic agent.
[0012] Preferably, when stirring and dissolving, the stirring temperature is 20 - 55°C, the stirring speed is 200 - 1000 rpm, and the stirring time is 5 - 30 min.
[0013] Preferably, when stirring and dissolving, the stirring temperature is 25 - 40°C, the stirring speed is 400 - 800 rpm, and the stirring time is 5 - 20 min.
[0014] Advantages of the present invention: The present invention studies a bacteriostatic agent for pets, and its preparation method has mild conditions and simple operation. The bacteriostatic agent mainly consists of nisin, supplemented by ε - polylysine hydrochloride and natamycin, and the bacteriostatic agent composed of trehalose as a wound - healing promoter, etc. has a broad - spectrum bactericidal effect and has the advantage of promoting wound healing.
[0015] The combined use of the three biological bacteriostatic agents, nisin, ε - polylysine hydrochloride, and natamycin, shows a significant synergistic effect in bacteriostatic action. This synergistic effect is not only reflected in the broad inhibition of bacteria and fungi, but also through multiple mechanisms such as destroying the integrity of the bacterial cell wall, increasing the permeability of the cell membrane, and interfering with intracellular metabolism and DNA function, etc., to achieve a strong control over the growth of microorganisms. Research shows that the combined use of nisin and ε - polylysine hydrochloride can already show synergistic antimicrobial activity against common pathogenic bacteria such as Staphylococcus aureus, Staphylococcus intermedius, Streptococcus, and Escherichia coli, and the addition of natamycin further expands the scope of this bacteriostatic effect, including the inhibition of various fungi such as Candida albicans.
[0016] It is also particularly important to promote wound healing while inhibiting bacteria. Trauma can damage the skin or mucous membranes, causing the body to lose its natural protective barrier. The wound surface is directly exposed to the air, making it vulnerable to repeated infections by pathogens, and thus prolonging the wound repair process. Therefore, secondary infection of wounds remains one of the important reasons why traumatic wounds are difficult to heal. To promote wound healing and relieve the pain of pets, the above compound preparation is specifically added with a wound healing promoter in this study, which can effectively prevent secondary infection of wounds.
[0017] Therefore, the bacteriostatic agent prepared by the present invention has good effects. By compounding multiple components, it synergistically enhances the effect, promotes wound healing, has a broad-spectrum bactericidal effect, and the bactericidal rate in vitro against Staphylococcus aureus, Escherichia coli, and Candida albicans can all reach 100%. It has good effects when used in pet hospitals. The product of the present invention has no obvious irritation to the skin of pets, has good safety, quick results, and is more convenient to use. Detailed implementation manners
[0018] The technical solutions in the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all of the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without making creative efforts shall fall within the protection scope of the present invention.
[0019] Example 1: Add nisin to water to keep the titer at 650 IU / mL; then sequentially add 0.02% ε-polylysine hydrochloride, 0.02% natamycin, and 2% trehalose according to mass percentage, stir and dissolve, then add an appropriate amount of glacial acetic acid to adjust the pH to 3.0, and finally add water to 100 mL; stir and dissolve for 10 min and filter under the conditions of a stirring temperature of 25 °C and a stirring speed of 450 rpm to obtain a bacteriostatic agent.
[0020] Example 2: Add nisin to water to keep the titer at 550 IU / mL; then sequentially add 0.03% ε-polylysine hydrochloride, 0.05% natamycin, and 3% trehalose according to mass percentage, stir and dissolve, then add an appropriate amount of glacial acetic acid to adjust the pH to 3.5, and finally add water to 100 mL; stir and dissolve for 8 min and filter under the conditions of a stirring temperature of 35 °C and a stirring speed of 550 rpm to obtain a bacteriostatic agent.
[0021] Example 3: Add nisin to water to keep the titer at 800 IU / mL; then add 0.04% ε-polylysine hydrochloride, 0.1% natamycin, and 4% trehalose in sequence by mass percentage, stir to dissolve, then add an appropriate amount of glacial acetic acid to adjust the pH to 5.0, and finally add water to 100 mL; stir and dissolve for 15 min at a stirring temperature of 25 °C and a stirring speed of 700 rpm, and then filter to obtain the bacteriostatic agent.
[0022] Example 4: Add nisin to water to keep the titer at 1000 IU / mL; then add 0.05% ε-polylysine hydrochloride, 0.08% natamycin, and 3.5% trehalose in sequence by mass percentage, stir to dissolve, then add an appropriate amount of glacial acetic acid to adjust the pH to 4.7, and finally add water to 100 mL; stir and dissolve for 18 min at a stirring temperature of 27 °C and a stirring speed of 750 rpm, and then filter to obtain the bacteriostatic agent.
[0023] Example 5: Add nisin to water to keep the titer at 1300 IU / mL; then add 0.03% ε-polylysine hydrochloride, 0.07% natamycin, and 2.5% trehalose in sequence by mass percentage, stir to dissolve, then add an appropriate amount of glacial acetic acid to adjust the pH to 3.9, and finally add water to 100 mL; stir and dissolve for 12 min at a stirring temperature of 35 °C and a stirring speed of 650 rpm, and then filter to obtain the bacteriostatic agent.
[0024] Example 6: Add nisin to water to keep the titer at 600 IU / mL; then add 0.02% ε-polylysine hydrochloride, 0.06% natamycin, and 2.5% trehalose in sequence by mass percentage, stir to dissolve, then add an appropriate amount of glacial acetic acid to adjust the pH to 4.5, and finally add water to 100 mL; stir and dissolve for 11 min at a stirring temperature of 40 °C and a stirring speed of 800 rpm, and then filter to obtain the bacteriostatic agent.
[0025] Detection test:
[0026] In vitro bacteriostatic test: Use pathogenic Escherichia coli, Staphylococcus aureus, and Candida albicans as the test bacteria, and use the Oxford cup double-layer agar diffusion method to conduct a bacteriostatic qualitative experiment. Add 10 mL of 2 g / mL water agar medium to a sterilized petri dish. After the water agar solidifies, place a sterilized Oxford cup in it. Add the indicator bacteria with a concentration of 10 6 ~10 7 cfu / mL to 20 mL of LB agar medium, mix well and pour it into the petri dish. After the medium solidifies, remove the Oxford cup, add 180 μL of the bacteriostatic agent to the hole, and culture at 37 °C for 24 h. Observe and measure the size of the inhibition zone. The results are as follows:
[0027] Table 1 In vitro antibacterial test results
[0028]
[0029] In vitro bactericidal test: Pathogenic Escherichia coli, Staphylococcus aureus, and Candida albicans were used as test bacteria. A quantitative bacteriostatic test was performed using the test tube method. The bacteriostatic agents were sterilized by filtration. 10 mL of each bacteriostatic agent was placed in separate test tubes. 0.1 mL of Staphylococcus aureus solution of appropriate concentration was added to each test tube. After mixing, 1 mL was transferred to a double dish. Trypticase soy agar medium was immediately poured into the dish. The bacteria were incubated at 37°C for 24 hours and then counted. The same procedure was used for Escherichia coli and Candida albicans. The bactericidal rates were observed and calculated. The results are as follows:
[0030] Table 2 In vitro bactericidal test results
[0031]
[0032] Use case for pet hospital:
[0033] Case 1
[0034] Patient dog: French bulldog, male, 3 years old
[0035] Symptoms: Interdigital inflammation, the dog frequently licks its toes, the skin of the feet becomes red, and in severe cases, there are pustules that smell bad and lameness.
[0036] Laboratory tests: fungi (Candida albicans+), bacteria (+).
[0037] Analysis of the cause: infection caused by bacteria and fungi.
[0038] Treatment: Example 6 antibacterial agent, three times daily, sprayed directly on the severely affected area. After seven days of continuous use, redness and swelling subsided, itching was alleviated, and exudate ceased. Repeat treatment for four weeks, a total of one course of treatment, and the wound healed, and the dog resumed normal walking and activities.
[0039] Case 2
[0040] Patient cat: Persian cat, female, 2 years old
[0041] Symptoms: Trauma and bleeding in the lower part of the neck.
[0042] Test results: Bacteria (-).
[0043] Analyze the cause of the disease: prevent bacterial infection and promote wound healing.
[0044] Treatment plan: Example 6 antibacterial agent, 3 times / day, spray directly on the severely affected area. After 28 days of continuous use, the wound did not become infected, the wound healed, and the skin and hair gradually grew back.
[0045] Case 3
[0046] Patient: Bedlington Terrier, male, 7 years old
[0047] Symptoms: Postoperative perianal tumor in elderly dog.
[0048] Test results: Bacteria (-).
[0049] Analyze the cause of the disease: prevent bacterial infection and promote wound healing.
[0050] Treatment plan: Use Example 6 antibacterial agent, 3 times a day, spray directly on the severely affected area. Use for 7 consecutive days, the wound will recover faster and prevent wound infection significantly.
[0051] Case 4
[0052] Patient cat: Ragdoll cat, female, 2 years old
[0053] Symptoms: Interdigital inflammation, frequent licking of the paws, and lameness. Examination of the interdigital spaces reveals redness, swelling, and pus-filled areas.
[0054] Laboratory tests: fungi (Candida albicans+), bacteria (+).
[0055] Analysis of the cause: infection caused by bacteria and fungi.
[0056] Treatment: The antibacterial agent from Example 6 was applied three times daily, directly to the severely affected area. After seven days of continuous use, redness and swelling subsided, and itching was alleviated. Repeat the treatment for four weeks, a total course of treatment, and the skin condition improved significantly, and the cat resumed normal walking and activities.
[0057] Conclusion: The results in Table 1 show that the pet antibacterial agents of Examples 1, 2, 5, and 6 have a good in vitro antibacterial effect. This is because the combined action of nisin, ε-polylysine hydrochloride, and natamycin creates a synergistic effect, resulting in a stronger inhibitory or killing effect on bacteria and fungi. The in vitro antibacterial effect of Examples 3 and 4 is poor, due to low concentrations or mismatched ratios of the main and auxiliary ingredients. The results in Table 2 show that the in vitro bactericidal rates of the pet antibacterial agents of Examples 1, 2, 5, and 6 all reached a high level, while the in vitro bactericidal effects of Examples 3 and 4 were poor.
[0058] The results of use cases in pet hospitals show that antibacterial agents for pets are effective in treating interdigital inflammation, trauma and postoperative wounds. They can not only effectively prevent bacterial and fungal infections and secondary infections, but also have a significant effect on promoting wound healing.
[0059] Through the above in vitro inhibition / bactericidal test and pet hospital use case test results, it can be seen that the pet antibacterial agent of the present invention has a good inhibition / bactericidal effect, can effectively promote wound healing, and has good clinical efficacy advantages.
[0060] It should be noted that in this text, relational terms such as first and second are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship or order between these entities or operations. Moreover, the terms "comprising", "including" or any other variant thereof are intended to cover non-exclusive inclusion, such that a process, method, article or device comprising a series of elements not only includes those elements but also includes other elements not expressly listed, or further includes elements inherent to such process, method, article or device.
[0061] Finally, it should be noted that the above are only preferred embodiments of the present invention and are not used to limit the present invention. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or perform equivalent replacements for some of the technical features. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included within the protection scope of the present invention.
Claims
1. An antibacterial agent for pets, characterized in that: Containing nisin with an effective titer of 500 - 2000 IU / mL.
2. The antibacterial agent for pets according to claim 1, characterized in that: In terms of mass percentage, the excipients of the bacteriostatic agent specifically include: 0.01 - 0.1% ε-polylysine hydrochloride, 0.02 - 0.2% natamycin, 2 - 8% trehalose, and an appropriate amount of glacial acetic acid is used to adjust the pH value, and the balance is water.
3. The bacteriostatic agent for pets according to claim 1, characterized in that: Containing nisin with an effective titer of 500 - 1500 IU / mL.
4. The bacteriostatic agent for pets according to claim 2, wherein: In terms of mass percentage, the excipients of the bacteriostatic agent specifically include: 0.02 - 0.05% ε-polylysine hydrochloride, 0.02 - 0.1% natamycin, 2 - 5% trehalose, and an appropriate amount of glacial acetic acid is used to adjust the pH value, and the balance is water.
5. A preparation method of an antibacterial agent for pets, characterized in that: Including the following steps: Add nisin, ε-polylysine hydrochloride, natamycin, and trehalose to water in sequence, stir to dissolve, then add an appropriate amount of glacial acetic acid to adjust the pH to 3.0 - 5.0, and filter to obtain the bacteriostatic agent.
6. The preparation method of an antibacterial agent for pets according to claim 5, characterized in that: Including the following steps: Add nisin with an effective titer of 500 - 1500 IU / mL, 0.02 - 0.05% ε-polylysine hydrochloride, 0.02 - 0.1% natamycin, and 2 - 5% trehalose to water in sequence, stir to dissolve, then add an appropriate amount of glacial acetic acid to adjust the pH to 3.0 - 5.0, and filter to obtain the bacteriostatic agent.
7. The preparation method of an antibacterial agent for pets according to claim 5 or 6, characterized in that: When stirring to dissolve, the stirring temperature is 20 - 55 °C, the stirring speed is 200 - 1000 rpm, and the stirring time is 5 - 30 min.
8. The preparation method of an antibacterial agent for pets according to claim 6, characterized in that: When stirring to dissolve, the stirring temperature is 25 - 40 °C, the stirring speed is 400 - 800 rpm, and the stirring time is 5 - 20 min.