Hydrogel with wound healing function and preparation method thereof

Abstract

The invention provides hydrogel with a wound healing function and a preparation method thereof.The hydrogel is prepared from, by mass, 50-70 parts of basic gel matrix, 20-30 parts of natural medicine extract and 8-10 parts of bioactive ingredients; the natural medicine extract comprises superfine pearl powder, a semen cassiae extract, an aloe extract, a cortex phellodendri extract, a honeysuckle flower extract, a dandelion extract, a centipede extract, a fructus forsythiae extract and a green tea extract; the bioactive components comprise epidermal growth factors, heparin sodium, mussel mucin and nano-silver ions; on the basis of a commercially available compound cortex phellodendri liniment, further research and development are carried out, the superfine pearl powder, the semen cassiae extract, the aloe extract, the green tea extract and other natural medicine components are added, the optimal proportion is selected, and the basic gel matrix, the bioactive components and the like are combined, so that the wound healing rate, the antibacterial rate and the water holding rate of the hydrogel are remarkably improved finally.

Description

Technical Field

[0001] The present invention relates to the technical field of wound repair, and more specifically, to a hydrogel with wound healing function and its preparation method. Background Art

[0002] Wound care is an important part of the medical field. Traditional wound care methods such as gauze dressing can protect the wound to a certain extent, but they often have problems such as easy infection, easy detachment, and secondary injury. Therefore, it is very necessary to develop an external medicine that can accelerate wound treatment.

[0003] Huangbai Liquid Coating Agent sold on the market has the effects of clearing heat and detoxifying, reducing swelling and removing necrosis. Its main ingredients include Forsythia suspensa, Phellodendron amurense, Lonicera japonica, Taraxacum mongolicum, Scolopendra subspinipes, etc., and it is prepared by processes such as decocting and concentrating these drugs.

[0004] However, the Huangbai Liquid Coating Agent prepared by this method is a water-based agent, which will cause secondary damage to the wound, and its treatment effect is not very ideal. At the same time, the problem of scarring cannot be solved for the wounds treated with Compound Huangbai Coating Agent.

[0005] In the field of biomedical materials, hydrogels are widely used in wound healing, tissue repair, drug release, etc. due to their unique physical and chemical properties. Therefore, the purpose of the present invention is to provide a wound healing hydrogel with comprehensive treatment effects and its preparation method. Summary of the Invention

[0006] To solve the above technical problems, the present invention discloses a hydrogel with wound healing function and its preparation method, specifically as follows: A hydrogel with wound healing function includes the following components, by mass: 50 - 70 parts of a basic gel matrix, 20 - 30 parts of a natural medicine extract, and 8 - 10 parts of a bioactive ingredient; The natural medicine extract includes ultrafine pearl powder, Cassia obtusifolia extract, Aloe vera extract, Phellodendron amurense extract, Lonicera japonica extract, Taraxacum mongolicum extract, Scolopendra subspinipes extract, Forsythia suspensa extract, and green tea extract; By mass, the ultrafine pearl powder is 8 - 12 parts, the Cassia obtusifolia extract is 5 - 8 parts, the Aloe vera extract is 15 - 20 parts, the Phellodendron amurense extract is 6 - 10 parts, the Lonicera japonica extract is 5 - 8 parts, the Taraxacum mongolicum extract is 5 - 10 parts, the Scolopendra subspinipes extract is 1 - 2 parts, the Forsythia suspensa extract is 5 - 10 parts, and the green tea extract is 5 - 10 parts; Among them, aloe-emodin and aloe polysaccharides in aloe extract, as well as alkaloids such as berberine and phellodendrine in phellodendron extract, all have significant antibacterial and anti-inflammatory effects. They can work synergistically to jointly inhibit the growth of various bacteria, reduce the inflammatory response of the wound, and thus accelerate the wound healing process; Both honeysuckle extract and dandelion extract contain a variety of antibacterial active ingredients, such as chlorogenic acid in honeysuckle and taraxasterol in dandelion, etc. They can also work synergistically to expand the antibacterial spectrum and enhance the control ability of wound infection; Ultra-fine pearl powder is rich in various minerals and trace elements, which can promote cell regeneration and repair. Aloe extract has moisturizing and repair effects, which can reduce skin dryness and discomfort. The combination of the two can work synergistically to accelerate the wound healing process and reduce the formation of scars.

[0007] Cassia seed extract can indirectly promote wound healing by regulating cell metabolism and promoting cell proliferation. The tea polyphenols in green tea extract have a strong antioxidant effect, which can scavenge free radicals and reduce the damage of oxidative stress to the wound. The synergistic effect of the two helps to improve the wound healing effect.

[0008] The bioactive components include epidermal growth factor, heparin sodium, mussel adhesive protein, and nano silver ions; By mass, the epidermal growth factor is 0.3 - 0.5 parts, the heparin sodium is 2 - 3 parts, the mussel adhesive protein is 4 - 6 parts, and the nano silver ions are 0.3 - 0.5 parts.

[0009] Among them, epidermal growth factor is a potent protein that promotes cell growth and differentiation, and plays an important role in the regeneration of epidermal cells during the wound healing process; Mussel adhesive protein has the functions of promoting cell adhesion, crawling substitution, and accelerating wound surface healing; The combination of the two can synergistically promote the proliferation and migration of epidermal cells, thus accelerating the wound healing process, The specific mechanism is as follows: Epidermal growth factor stimulates the receptors on the cell surface, activates a series of intracellular signaling pathways, and promotes cell proliferation and differentiation. Mussel adhesive protein provides a favorable environment for cell proliferation through its unique adhesion and cell adhesion promoting properties. The two complement each other and work together during the wound healing process.

[0010] Heparin sodium can interfere with the blood coagulation process and reduce thrombus formation, thus facilitating blood supply and nutrient delivery to the wound site; Nano silver ions have a broad-spectrum antibacterial effect and can effectively inhibit bacterial infection at the wound site; The combination of the two can promote blood circulation and nutrient supply to the wound while keeping the wound clean, and accelerate the healing process.

[0011] The specific mechanism is as follows: Heparin sodium improves blood circulation at the wound site through its anticoagulant effect, providing sufficient nutrition and oxygen for wound healing; nano silver ions reduce the number of bacteria at the wound site through their antibacterial effect, reducing the risk of infection. The two work synergistically in maintaining wound cleanliness and promoting healing.

[0012] Mussel adhesive protein forms a protective film on the wound surface, which has the properties of waterproof, breathable and antibacterial; nano silver ions further enhance the antibacterial ability of the wound through their broad-spectrum antibacterial effect; when used in combination, they can form a powerful antibacterial barrier on the wound surface, effectively preventing bacterial infection and promoting wound healing; The specific mechanism is as follows: Mussel adhesive protein forms a protective film on the wound surface through its unique molecular structure and adhesion performance. This protective film not only has the properties of waterproof and breathable, but also can interact with nano silver ions to enhance their antibacterial effect. Nano silver ions achieve the bactericidal effect by destroying the bacterial cell membrane and inhibiting bacterial metabolism.

[0013] Preferably, the preparation method of the cassia seed extract / aloe extract / amur corktree bark extract / honeysuckle flower extract / dandelion extract / centipede extract / forsythia fruit extract / green tea extract is as follows: Step 1: Respectively crush cassia seeds / aloe / amur corktree bark / honeysuckle flowers / dandelions / centipedes / forsythia fruits / green tea leaves with a pulverizer to obtain cassia seed powder / aloe powder / amur corktree bark powder / honeysuckle flower powder / dandelion powder / centipede powder / forsythia fruit powder / green tea leaf powder respectively; Step 2: Respectively add cassia seed powder / aloe powder / amur corktree bark powder / honeysuckle flower powder / dandelion powder / centipede powder / forsythia fruit powder / green tea leaf powder to a supercritical extractor; Step 3: Pump carbon dioxide liquid into the supercritical extractor and extract for 90 - 110 min to obtain supercritical carbon dioxide containing cassia seeds / supercritical carbon dioxide containing aloe / supercritical carbon dioxide containing amur corktree bark / supercritical carbon dioxide containing honeysuckle flowers / supercritical carbon dioxide containing dandelions / supercritical carbon dioxide containing centipedes / supercritical carbon dioxide containing forsythia fruits / supercritical carbon dioxide containing green tea leaves respectively; Step 4: Respectively introduce supercritical carbon dioxide containing cassia seeds / supercritical carbon dioxide containing aloe / supercritical carbon dioxide containing amur corktree bark / supercritical carbon dioxide containing honeysuckle flowers / supercritical carbon dioxide containing dandelions / supercritical carbon dioxide containing centipedes / supercritical carbon dioxide containing forsythia fruits / supercritical carbon dioxide containing green tea leaves into a separator to separately separate out cassia seed extract / aloe extract / amur corktree bark extract / honeysuckle flower extract / dandelion extract / centipede extract / forsythia fruit extract / green tea extract.

[0014] Preferably, the preparation method of the cassia seed extract / aloe extract / amur corktree bark extract / honeysuckle flower extract / dandelion extract / centipede extract / forsythia fruit extract / green tea extract is as follows: Step 1: After cleaning and drying cassia seeds / aloe / amur corktree bark / honeysuckle flowers / dandelions / centipedes / forsythia fruits / green tea leaves respectively, they are pulverized to a size between 50 and 80 microns to obtain micron-sized cassia seed powder / micron-sized aloe powder / micron-sized amur corktree bark powder / micron-sized honeysuckle flower powder / micron-sized dandelion powder / micron-sized centipede powder / micron-sized forsythia fruit powder / micron-sized green tea leaf powder; Step 2: The micron-sized cassia seed powder / micron-sized aloe powder / micron-sized amur corktree bark powder / micron-sized honeysuckle flower powder / micron-sized dandelion powder / micron-sized centipede powder / micron-sized forsythia fruit powder / micron-sized green tea leaf powder is mixed with deionized water to form a cassia seed suspension / aloe suspension / amur corktree bark suspension / honeysuckle flower suspension / dandelion suspension / centipede suspension / forsythia fruit suspension / green tea suspension; Step 3: The cassia seed suspension / aloe suspension / amur corktree bark suspension / honeysuckle flower suspension / dandelion suspension / centipede suspension / forsythia fruit suspension / green tea suspension is respectively placed in a high-voltage pulsed electric field and subjected to high-voltage pulse treatment for 10 - 15 minutes; Step 4: The cassia seed suspension / aloe suspension / amur corktree bark suspension / honeysuckle flower suspension / dandelion suspension / centipede suspension / forsythia fruit suspension / green tea suspension after high-voltage pulse treatment is filtered and separated respectively to obtain cassia seed extract / aloe extract / amur corktree bark extract / honeysuckle flower extract / dandelion extract / centipede extract / forsythia fruit extract / green tea extract.

[0015] Preferably, the electric field strength of the high-voltage pulsed electric field is 30 - 40 kV / cm and the frequency is 100 - 150 Hz.

[0016] Preferably, the particle size of the ultrafine pearl powder is less than or equal to 10 microns.

[0017] Preferably, the basic gel matrix includes sodium hyaluronate, sodium alginate, collagen, polyacrylamide, polyvinyl alcohol, carbomer, nano calcium alginate; By mass, the sodium hyaluronate is 5 - 10 parts, the sodium alginate is 10 - 20 parts, the collagen is 10 - 15 parts, the polyacrylamide is 5 - 8 parts, the polyvinyl alcohol is 10 - 15 parts, the carbomer is 2 - 3 parts, and the nano calcium alginate is 3 - 5 parts.

[0018] Preferably, the preparation method of the hydrogel for wound healing is as follows: S1. Take sodium hyaluronate, sodium alginate, collagen, polyacrylamide, polyvinyl alcohol, and carbomer, dissolve each of them in deionized water, heat and continuously stir to make them uniformly dissolved, forming their respective matrix solutions; S2. Uniformly disperse calcium alginate nanoparticles in deionized water to form a calcium alginate nanoparticle suspension; S3. Mix the matrix solutions and the calcium alginate nanoparticle suspension, heat and continuously stir until a uniform basic gel matrix is formed; S4. Take ultrafine pearl powder, cassia seed extract, aloe extract, phellodendron extract, honeysuckle extract, dandelion extract, centipede extract, forsythia extract, and green tea extract, disperse each of them in deionized water to form their respective natural medicine extract solutions; S5. Slowly add the natural medicine extract solution prepared in step S4 to the basic gel matrix prepared in step S3, heat and continuously stir to make them uniformly mixed, obtaining a pretreated gel; S6. Dissolve epidermal growth factor, heparin sodium, and mussel adhesive protein in physiological saline respectively to form their respective bioactive ingredient solutions; S7. Add silver nanoparticles to physiological saline to make them uniformly dispersed, forming a silver nanoparticle suspension; S8. Slowly add the bioactive ingredient solutions prepared in step S6 and the silver nanoparticle suspension prepared in step S7 to the pretreated gel prepared in step S5, heat and continuously stir to make them uniformly mixed, obtaining a hydrogel for wound healing.

[0019] Preferably, in steps S1, S3, S5, and S8, the stirring speed is 200 - 400 rpm / min, and the heating temperature is 30 - 35 °C.

[0020] Preferably, in step S1, the material - liquid ratios of sodium hyaluronate, sodium alginate, collagen, polyacrylamide, polyvinyl alcohol, and carbomer to deionized water are 1:4 - 6, 1:10 - 13, 1:10 - 12, 1:6 - 7, 1:2 - 3, and 1:2 - 3 respectively; In step S2, the material - liquid ratio of calcium alginate nanoparticles to deionized water is 1:4 - 6; In step S4, the ratios of ultrafine pearl powder, cassia seed extract, aloe extract, phellodendron extract, honeysuckle extract, dandelion extract, centipede extract, forsythia extract, and green tea extract to deionized water are 1 - 2:10, 1 - 2:10, 1 - 2:10, 1 - 2:10, 1 - 2:10, 1 - 2:10, 1 - 2:20, 1 - 2:10, and 1 - 2:10 respectively.

[0021] Preferably, in the step S6, the material ratio of epidermal growth factor, heparin sodium, mussel adhesive protein to physiological saline is 1-2:10, 2-3:10, 2-3:10; In the step S7, the material ratio of nano silver ions to physiological saline is 1-2:10.

[0022] Cassia seed, aloe, phellodendron bark, honeysuckle flower, dandelion, centipede, forsythia suspensa, green tea leaves mentioned in the present invention are all Chinese herbal pieces.

[0023] Compared with the prior art, the beneficial effects of the present invention are as follows: 1. The hydrogel for wound healing provided by the present invention can closely adhere to the wound surface, is not easy to fall off, will not cause secondary damage to the wound, and improves the comfort of patients compared with the water coating agent in the prior art; at the same time, the hydrogel for wound healing provided by the present invention realizes multi-path and multi-target therapeutic effects through the reasonable proportioning of various natural medicine extracts and bioactive components, and has a more comprehensive therapeutic effect compared with the compound phellodendron bark coating agent with a single component.

[0024] 2. The hydrogel for wound healing provided by the present invention contains 20-30 parts of natural medicine extracts. The natural medicine extracts include 8-12 parts of ultrafine pearl powder, 5-8 parts of cassia seed extract, 15-20 parts of aloe extract, 6-10 parts of phellodendron bark extract, 5-8 parts of honeysuckle flower extract, 5-10 parts of dandelion extract, 1-2 parts of centipede extract, 5-10 parts of forsythia suspensa extract, 5-10 parts of green tea extract, which significantly improves its various biological activities such as anti-inflammatory, antibacterial, and promoting cell regeneration, and helps to accelerate the wound healing process; Among them, aloe emodin and aloe polysaccharide in the aloe extract, as well as alkaloids such as berberine and phellodendrine in the phellodendron bark extract, all have significant antibacterial and anti-inflammatory effects. They can synergistically enhance the effect and jointly inhibit the growth of various bacteria, reduce the inflammatory reaction of the wound, and thus accelerate the wound healing process; Both the honeysuckle flower extract and the dandelion extract contain various antibacterial active ingredients, such as chlorogenic acid in honeysuckle flower and taraxasterol in dandelion, etc. They can also act synergistically to expand the antibacterial spectrum and enhance the control ability of wound infection; The ultrafine pearl powder is rich in various minerals and trace elements, which can promote the regeneration and repair of cells. The aloe extract has a moisturizing and repairing effect, which can reduce skin dryness and discomfort. The combination of the two can synergistically enhance the effect to accelerate the wound healing process and reduce the formation of scars.

[0025] Cassia seed extract can indirectly promote wound healing by regulating cell metabolism, promoting cell proliferation, etc. Polyphenols in green tea extract have a strong antioxidant effect, can scavenge free radicals, and reduce the damage of oxidative stress to wounds. The synergistic effect of the two helps to improve the wound healing effect.

[0026] 3. The present invention does not adopt the traditional Chinese medicine decoction method for extracting Chinese medicine components, but provides two methods, supercritical carbon dioxide extraction method and high-voltage pulse extraction method, for extracting Cassia seed extract, Aloe vera extract, Phellodendron amurense extract, Lonicera japonica extract, Taraxacum mongolicum extract, Scolopendra subspinipes extract, Forsythia suspensa extract, and green tea extract. The obtained natural medicine extracts are purer and have more significant medicinal effects than the traditional method.

[0027] 4. The hydrogel for wound healing provided by the present invention contains 8-10 parts of bioactive components. The bioactive components include 0.3-0.5 parts of epidermal growth factor, 2-3 parts of heparin sodium, 4-6 parts of mussel adhesive protein, and 0.3-0.5 parts of nano silver ions. Through the above reasonably proportioned bioactive components, while promoting wound healing, it can also reduce scar formation, prevent infection, and reduce the risk of complications; Among them, epidermal growth factor is a potent protein that promotes cell growth and differentiation and plays an important role in the regeneration of epidermal cells during the wound healing process; mussel adhesive protein has the functions of promoting cell adhesion, crawling substitution, and accelerating wound surface healing; the combination of the two can synergistically promote the proliferation and migration of epidermal cells, thus accelerating the wound healing process; Heparin sodium can interfere with the blood coagulation process and reduce thrombus formation, thus facilitating blood supply and nutrient delivery to the wound site; nano silver ions have a broad-spectrum antibacterial effect and can effectively inhibit bacterial infection at the wound site; the combination of the two can promote blood circulation and nutrient supply to the wound while keeping the wound clean, accelerating the healing process; Mussel adhesive protein forms a protective film on the wound surface, which has the characteristics of waterproof, breathable, and antibacterial; nano silver ions further enhance the antibacterial ability of the wound through its broad-spectrum antibacterial effect; the combination of the two can form a powerful antibacterial barrier on the wound surface, effectively preventing bacterial infection and promoting wound healing.

[0028] 5. The present invention is further developed on the basis of the commercially available compound Phellodendron amurense liniment, adding natural medicine components such as ultrafine pearl powder, Cassia seed extract, Aloe vera extract, and green tea extract, selecting the optimal ratio, and combining with the basic gel matrix, bioactive components, etc. Finally, the wound healing rate, antibacterial rate, and water retention rate of the hydrogel are significantly improved. Detailed implementation mode

[0029] Example 1 A hydrogel with wound healing function, comprising the following components by mass: 50 parts of a basic gel matrix, 20 parts of a natural medicine extract, and 8 parts of a bioactive ingredient; The natural medicine extract includes ultrafine pearl powder, semen cassiae extract, aloe extract, phellodendron amurense extract, honeysuckle flower extract, dandelion extract, scolopendra extract, forsythia suspense extract, and green tea extract; By mass, the ultrafine pearl powder is 8 parts, the semen cassiae extract is 5 parts, the aloe extract is 15 parts, the phellodendron amurense extract is 6 parts, the honeysuckle flower extract is 5 parts, the dandelion extract is 5 parts, the scolopendra extract is 1 part, the forsythia suspense extract is 5 parts, and the green tea extract is 5 parts; The bioactive ingredient includes epidermal growth factor, heparin sodium, mussel adhesive protein, and nano silver ions; By mass, the epidermal growth factor is 0.3 parts, the heparin sodium is 2 parts, the mussel adhesive protein is 4 parts, and the nano silver ions are 0.3 parts.

[0030] Moreover, the preparation method of the semen cassiae extract / aloe extract / phellodendron amurense extract / honeysuckle flower extract / dandelion extract / scolopendra extract / forsythia suspense extract / green tea extract is as follows: Step 1: Respectively pulverize semen cassiae / aloe / phellodendron amurense / honeysuckle flower / dandelion / scolopendra / forsythia suspense / green tea leaves with a pulverizer to obtain semen cassiae powder / aloe powder / phellodendron amurense powder / honeysuckle flower powder / dandelion powder / scolopendra powder / forsythia suspense powder / green tea leaf powder; Step 2: Respectively add the semen cassiae powder / aloe powder / phellodendron amurense powder / honeysuckle flower powder / dandelion powder / scolopendra powder / forsythia suspense powder / green tea leaf powder to a supercritical extractor; Step 3: Pump carbon dioxide liquid into the supercritical extractor and extract for 90 minutes to obtain supercritical carbon dioxide containing semen cassiae / supercritical carbon dioxide containing aloe / supercritical carbon dioxide containing phellodendron amurense / supercritical carbon dioxide containing honeysuckle flower / supercritical carbon dioxide containing dandelion / supercritical carbon dioxide containing scolopendra / supercritical carbon dioxide containing forsythia suspense / supercritical carbon dioxide containing green tea; Step 4: Respectively introduce the supercritical carbon dioxide containing semen cassiae / supercritical carbon dioxide containing aloe / supercritical carbon dioxide containing phellodendron amurense / supercritical carbon dioxide containing honeysuckle flower / supercritical carbon dioxide containing dandelion / supercritical carbon dioxide containing scolopendra / supercritical carbon dioxide containing forsythia suspense / supercritical carbon dioxide containing green tea into a separator to separately separate out the semen cassiae extract / aloe extract / phellodendron amurense extract / honeysuckle flower extract / dandelion extract / scolopendra extract / forsythia suspense extract / green tea extract.

[0031] Moreover, the particle size of the ultrafine pearl powder is less than or equal to 10 microns.

[0032] Moreover, the basic gel matrix includes sodium hyaluronate, sodium alginate, collagen, polyacrylamide, polyvinyl alcohol, carbomer, and nano calcium alginate; By mass, the sodium hyaluronate is 5 parts, the sodium alginate is 10 parts, the collagen is 10 parts, the polyacrylamide is 5 parts, the polyvinyl alcohol is 10 parts, the carbomer is 2 parts, and the nano calcium alginate is 3 parts.

[0033] Moreover, the preparation method of the hydrogel for wound healing is as follows: S1. Take sodium hyaluronate, sodium alginate, collagen, polyacrylamide, polyvinyl alcohol, and carbomer, dissolve each of them in deionized water, heat and continuously stir to make them uniformly dissolved, forming respective matrix solutions; S2. Uniformly disperse nano calcium alginate in deionized water to form a nano calcium alginate suspension; S3. Mix the matrix solutions and the nano calcium alginate suspension, heat and continuously stir until a uniform basic gel matrix is formed; S4. Take ultrafine pearl powder, cassia seed extract, aloe extract, phellodendron bark extract, honeysuckle extract, dandelion extract, centipede extract, forsythia extract, and green tea extract, disperse each of them in deionized water to form respective natural medicine extract solutions; S5. Slowly add the natural medicine extract solutions prepared in step S4 to the basic gel matrix prepared in step S3, heat and continuously stir to make them uniformly mixed, obtaining a pretreated gel; S6. Dissolve epidermal growth factor, heparin sodium, and mussel adhesive protein in physiological saline respectively to form respective bioactive ingredient solutions; S7. Add nano silver ions to physiological saline to make them uniformly dispersed, forming a nano silver ion suspension; S8. Slowly add the bioactive ingredient solutions prepared in step S6 and the nano silver ion suspension prepared in step S7 to the pretreated gel prepared in step S5, heat and continuously stir to make them uniformly mixed, obtaining the hydrogel for wound healing.

[0034] Moreover, in steps S1, S3, S5, and S8, the stirring speed is 200 rpm / min, and the heating temperature is 30°C.

[0035] Moreover, in step S1, the material-liquid ratios of sodium hyaluronate, sodium alginate, collagen, polyacrylamide, polyvinyl alcohol, carbomer to deionized water are 1:4, 1:10, 1:10, 1:6, 1:2, 1:2 respectively; In the step S2, the material ratio of nano calcium alginate to ionic water is 1:4; In the step S4, the ratio of the ultrafine pearl powder, cassia seed extract, aloe extract, phellodendron extract, honeysuckle extract, dandelion extract, centipede extract, forsythia extract, green tea extract to deionized water is 1:10, 1:10, 1:10, 1:10, 1:10, 1:10, 1:20, 1:10, 1:10.

[0036] Moreover, in the step S6, the material ratio of epidermal growth factor, heparin sodium, mussel adhesive protein to physiological saline is 1:10, 2:10, 2:10.

[0037] Moreover, in the step S7, the material ratio of nano silver ions to physiological saline is 1:10.

[0038] Example 2 A hydrogel with wound healing function, comprising the following components in parts by mass: 70 parts of a basic gel matrix, 30 parts of a natural medicine extract, and 10 parts of a bioactive ingredient; The natural medicine extract includes ultrafine pearl powder, cassia seed extract, aloe extract, phellodendron extract, honeysuckle extract, dandelion extract, centipede extract, forsythia extract, green tea extract; In parts by mass, the ultrafine pearl powder is 12 parts, the cassia seed extract is 8 parts, the aloe extract is 20 parts, the phellodendron extract is 10 parts, the honeysuckle extract is 8 parts, the dandelion extract is 10 parts, the centipede extract is 2 parts, the forsythia extract is 10 parts, and the green tea extract is 10 parts; The bioactive ingredient includes epidermal growth factor, heparin sodium, mussel adhesive protein, nano silver ions; In parts by mass, the epidermal growth factor is 0.5 parts, the heparin sodium is 3 parts, the mussel adhesive protein is 6 parts, and the nano silver ions are 0.5 parts.

[0039] Moreover, the preparation method of the cassia seed extract / aloe extract / phellodendron extract / honeysuckle extract / dandelion extract / centipede extract / forsythia extract / green tea extract is as follows: Step 1: Respectively crush cassia seeds / aloe / phellodendron / honeysuckle / dandelion / centipede / forsythia / green tea leaves with a pulverizer to obtain cassia seed powder / aloe powder / phellodendron powder / honeysuckle powder / dandelion powder / centipede powder / forsythia powder / green tea leaf powder; Step 2: Respectively add the cassia seed powder / aloe powder / phellodendron powder / honeysuckle powder / dandelion powder / centipede powder / forsythia powder / green tea leaf powder to a supercritical extractor; Step 3: Pump liquid carbon dioxide into the supercritical extractor and extract for 110 minutes to obtain supercritical carbon dioxide containing cassia seed / supercritical carbon dioxide containing aloe / supercritical carbon dioxide containing phellodendron bark / supercritical carbon dioxide containing honeysuckle / supercritical carbon dioxide containing dandelion / supercritical carbon dioxide containing centipede / supercritical carbon dioxide containing forsythia / supercritical carbon dioxide containing green tea respectively; Step 4: Respectively introduce supercritical carbon dioxide containing cassia seed / supercritical carbon dioxide containing aloe / supercritical carbon dioxide containing phellodendron bark / supercritical carbon dioxide containing honeysuckle / supercritical carbon dioxide containing dandelion / supercritical carbon dioxide containing centipede / supercritical carbon dioxide containing forsythia / supercritical carbon dioxide containing green tea into the separator to separately separate out cassia seed extract / aloe extract / phellodendron bark extract / honeysuckle extract / dandelion extract / centipede extract / forsythia extract / green tea extract.

[0040] Moreover, the particle size of the ultrafine pearl powder is less than or equal to 10 microns.

[0041] Moreover, the basic gel matrix includes sodium hyaluronate, sodium alginate, collagen, polyacrylamide, polyvinyl alcohol, carbomer, nano calcium alginate; By mass, the sodium hyaluronate is 10 parts, the sodium alginate is 20 parts, the collagen is 15 parts, the polyacrylamide is 8 parts, the polyvinyl alcohol is 15 parts, the carbomer is 3 parts, and the nano calcium alginate is 5 parts.

[0042] Moreover, the preparation method of the hydrogel for wound healing is as follows: S1: Take sodium hyaluronate, sodium alginate, collagen, polyacrylamide, polyvinyl alcohol, carbomer, dissolve each of them in deionized water, heat and continuously stir to make them dissolve evenly to form their respective matrix solutions; S2: Uniformly disperse nano calcium alginate in deionized water to form a nano calcium alginate suspension; S3: Mix the matrix solutions and the nano calcium alginate suspension, heat and continuously stir until a uniform basic gel matrix is formed; S4: Take ultrafine pearl powder, cassia seed extract, aloe extract, phellodendron bark extract, honeysuckle extract, dandelion extract, centipede extract, forsythia extract, green tea extract, disperse each of them in deionized water to form their respective natural medicine extract solutions; S5: Slowly add the natural medicine extract solutions prepared in step S4 to the basic gel matrix prepared in step S3, heat and continuously stir to make them mix evenly to obtain a pretreated gel; S6: Dissolve epidermal growth factor, heparin sodium, and mussel adhesive protein in physiological saline respectively to form their respective bioactive ingredient solutions; S7. Add silver nanoparticles into normal saline to make it evenly dispersed, forming a silver nanoparticle suspension; S8. Slowly add the bioactive ingredient solution prepared in step S6 and the silver nanoparticle suspension prepared in step S7 into the pretreated gel prepared in step S5, heat and continuously stir to make them evenly mixed, obtaining a hydrogel for wound healing.

[0043] Moreover, in steps S1, S3, S5, and S8, the stirring speed is 400 rpm / min and the heating temperature is 35°C.

[0044] Moreover, in step S1, the material-liquid ratios of sodium hyaluronate, sodium alginate, collagen, polyacrylamide, polyvinyl alcohol, carbomer to ionized water are 1:6, 1:13, 1:12, 1:7, 1:3, 1:3 respectively; In step S2, the material-liquid ratio of calcium alginate nanoparticles to ionized water is 1:6; In step S, the ratios of the ultrafine pearl powder, cassia seed extract, aloe extract, phellodendron bark extract, honeysuckle extract, dandelion extract, centipede extract, forsythia extract, green tea extract to deionized water are 2:10, 2:10, 2:10, 2:10, 2:10, 2:10, 2:20, 2:10, 2:10.

[0045] Moreover, in step S6, the material-liquid ratios of epidermal growth factor, heparin sodium, mussel adhesive protein to normal saline are 2:10, 3:10, 3:10.

[0046] Moreover, in step S7, the material-liquid ratio of silver nanoparticles to normal saline is 2:10.

[0047] Example 3 A hydrogel for wound healing, comprising the following components in parts by mass: 60 parts of a basic gel matrix, 25 parts of a natural medicine extract, and 9 parts of bioactive ingredients; The natural medicine extract includes ultrafine pearl powder, cassia seed extract, aloe extract, phellodendron bark extract, honeysuckle extract, dandelion extract, centipede extract, forsythia extract, green tea extract; In parts by mass, the ultrafine pearl powder is 10 parts, the cassia seed extract is 6.5 parts, the aloe extract is 18 parts, the phellodendron bark extract is 8 parts, the honeysuckle extract is 6.5 parts, the dandelion extract is 8 parts, the centipede extract is 1.5 parts, the forsythia extract is 8, and the green tea extract is 8; The bioactive ingredients include epidermal growth factor, heparin sodium, mussel adhesive protein, silver nanoparticles; By mass fraction, the epidermal growth factor is 0.4 parts, the heparin sodium is 2.5 parts, the mussel adhesive protein is 5 parts, and the nano silver ions are 0.4 parts.

[0048] Moreover, the preparation method of the cassia seed extract / aloe extract / amur cork tree bark extract / honeysuckle extract / dandelion extract / centipede extract / forsythia extract / green tea extract is as follows: Step 1: Respectively crush cassia seeds / aloe / amur cork tree bark / honeysuckle / dandelion / centipede / forsythia / green tea leaves with a pulverizer to obtain cassia seed powder / aloe powder / amur cork tree bark powder / honeysuckle powder / dandelion powder / centipede powder / forsythia powder / green tea leaf powder respectively; Step 2: Respectively add cassia seed powder / aloe powder / amur cork tree bark powder / honeysuckle powder / dandelion powder / centipede powder / forsythia powder / green tea leaf powder to a supercritical extractor; Step 3: Pump carbon dioxide liquid into the supercritical extractor and extract for 100 min to obtain supercritical carbon dioxide containing cassia seeds / supercritical carbon dioxide containing aloe / supercritical carbon dioxide containing amur cork tree bark / supercritical carbon dioxide containing honeysuckle / supercritical carbon dioxide containing dandelion / supercritical carbon dioxide containing centipede / supercritical carbon dioxide containing forsythia / supercritical carbon dioxide containing green tea respectively; Step 4: Respectively introduce supercritical carbon dioxide containing cassia seeds / supercritical carbon dioxide containing aloe / supercritical carbon dioxide containing amur cork tree bark / supercritical carbon dioxide containing honeysuckle / supercritical carbon dioxide containing dandelion / supercritical carbon dioxide containing centipede / supercritical carbon dioxide containing forsythia / supercritical carbon dioxide containing green tea into a separator to separately separate out cassia seed extract / aloe extract / amur cork tree bark extract / honeysuckle extract / dandelion extract / centipede extract / forsythia extract / green tea extract.

[0049] Moreover, the particle size of the ultrafine pearl powder is less than or equal to 10 microns.

[0050] Moreover, the basic gel matrix includes sodium hyaluronate, sodium alginate, collagen, polyacrylamide, polyvinyl alcohol, carbomer, nano calcium alginate; By mass fraction, the sodium hyaluronate is 8 parts, the sodium alginate is 15 parts, the collagen is 13 parts, the polyacrylamide is 6.5 parts, the polyvinyl alcohol is 13 parts, the carbomer is 2.5 parts, and the nano calcium alginate is 4 parts.

[0051] Moreover, the preparation method of the hydrogel for wound healing is as follows: S1: Take sodium hyaluronate, sodium alginate, collagen, polyacrylamide, polyvinyl alcohol, carbomer, dissolve each of them in deionized water, heat and continuously stir to make them uniformly dissolved to form their respective matrix solutions; S2. Uniformly disperse calcium alginate nanoparticles in deionized water to form a calcium alginate nanoparticle suspension; S3. Mix the matrix solution and the calcium alginate nanoparticle suspension, heat and continuously stir until a uniform basic gel matrix is formed; S4. Take ultrafine pearl powder, cassia seed extract, aloe extract, phellodendron bark extract, honeysuckle extract, dandelion extract, centipede extract, forsythia extract, and green tea extract, and disperse each of them in deionized water to form their respective natural medicine extract solutions; S5. Slowly add the natural medicine extract solution prepared in step S4 to the basic gel matrix prepared in step S3, heat and continuously stir to make them uniformly mixed to obtain a pretreated gel; S6. Dissolve epidermal growth factor, heparin sodium, and mussel adhesive protein in physiological saline respectively to form their respective bioactive ingredient solutions; S7. Add silver nanoparticles to physiological saline to make them uniformly dispersed to form a silver nanoparticle suspension; S8. Slowly add the bioactive ingredient solutions prepared in step S6 and the silver nanoparticle suspension prepared in step S7 to the pretreated gel prepared in step S5, heat and continuously stir to make them uniformly mixed to obtain a hydrogel for wound healing.

[0052] Moreover, in steps S1, S3, S5, and S8, the stirring speed is 300 rpm / min, and the heating temperature is 33 °C.

[0053] Moreover, in step S1, the material-liquid ratios of sodium hyaluronate, sodium alginate, collagen, polyacrylamide, polyvinyl alcohol, carbomer to ionic water are 1:5, 1:12, 1:11, 1:6.5, 1:2.5, 1:2.5 respectively; In step S2, the material-liquid ratio of calcium alginate nanoparticles to ionic water is 1:5; In step S4, the ratios of the ultrafine pearl powder, cassia seed extract, aloe extract, phellodendron bark extract, honeysuckle extract, dandelion extract, centipede extract, forsythia extract, and green tea extract to deionized water are 1.5:10, 1.5:10, 1.5:10, 1.5:10, 1.5:10, 1.5:10, 1.5:20, 1.5:10, 1.5:10.

[0054] Moreover, in step S6, the material-liquid ratios of the epidermal growth factor, heparin sodium, and mussel adhesive protein to physiological saline are 1.5:10, 2.5:10, 2.5:10; In step S7, the material-liquid ratio of silver nanoparticles to physiological saline is 1.5:10.

[0055] Example 4 The difference between this example and Example 3 lies in the different preparation methods of the cassia seed extract / aloe extract / amur corktree bark extract / honeysuckle flower extract / dandelion extract / centipede extract / forsythia fruit extract / green tea extract, which are as follows: Step 1: After cleaning and drying cassia seeds / aloe / amur corktree bark / honeysuckle flowers / dandelions / centipedes / forsythia fruits / green tea leaves respectively, they are pulverized to within 50 microns to obtain micron-level cassia seed powder / micron-level aloe powder / micron-level amur corktree bark powder / micron-level honeysuckle flower powder / micron-level dandelion powder / micron-level centipede powder / micron-level forsythia fruit powder / micron-level green tea leaf powder; Step 2: The micron-level cassia seed powder / micron-level aloe powder / micron-level amur corktree bark powder / micron-level honeysuckle flower powder / micron-level dandelion powder / micron-level centipede powder / micron-level forsythia fruit powder / micron-level green tea leaf powder are mixed with deionized water to form cassia seed suspension / aloe suspension / amur corktree bark suspension / honeysuckle flower suspension / dandelion suspension / centipede suspension / forsythia fruit suspension / green tea suspension; Step 3: The cassia seed suspension / aloe suspension / amur corktree bark suspension / honeysuckle flower suspension / dandelion suspension / centipede suspension / forsythia fruit suspension / green tea suspension are respectively placed in a high-voltage pulsed electric field and subjected to high-voltage pulse treatment for 10 minutes; Step 4: The cassia seed suspension / aloe suspension / amur corktree bark suspension / honeysuckle flower suspension / dandelion suspension / centipede suspension / forsythia fruit suspension / green tea suspension after high-voltage pulse treatment are filtered and separated respectively to obtain cassia seed extract / aloe extract / amur corktree bark extract / honeysuckle flower extract / dandelion extract / centipede extract / forsythia fruit extract / green tea extract.

[0056] Moreover, the electric field strength of the high-voltage pulsed electric field is 30 kV / cm and the frequency is 100 Hz.

[0057] Example 5 The difference between this example and Example 3 lies in the different preparation methods of the cassia seed extract / aloe extract / amur corktree bark extract / honeysuckle flower extract / dandelion extract / centipede extract / forsythia fruit extract / green tea extract, which are as follows: Step 1: After cleaning and drying cassia seeds / aloe / amur corktree bark / honeysuckle flowers / dandelions / centipedes / forsythia fruits / green tea leaves respectively, they are pulverized to within 80 microns to obtain micron-level cassia seed powder / micron-level aloe powder / micron-level amur corktree bark powder / micron-level honeysuckle flower powder / micron-level dandelion powder / micron-level centipede powder / micron-level forsythia fruit powder / micron-level green tea leaf powder; Step 2: After mixing micron-sized semen cassiae powder / micron-sized aloe vera powder / micron-sized phellodendron amurense powder / micron-sized honeysuckle flower powder / micron-sized dandelion powder / micron-sized centipede powder / micron-sized forsythia suspense powder / micron-sized green tea leaf powder with deionized water, form semen cassiae suspension / aloe vera suspension / phellodendron amurense suspension / honeysuckle flower suspension / dandelion suspension / centipede suspension / forsythia suspense suspension / green tea leaf suspension; Step 3: Respectively place the semen cassiae suspension / aloe vera suspension / phellodendron amurense suspension / honeysuckle flower suspension / dandelion suspension / centipede suspension / forsythia suspense suspension / green tea leaf suspension in a high-voltage pulsed electric field and perform high-voltage pulse treatment for 15 minutes; Step 4: Respectively filter and separate the semen cassiae suspension / aloe vera suspension / phellodendron amurense suspension / honeysuckle flower suspension / dandelion suspension / centipede suspension / forsythia suspense suspension / green tea leaf suspension after high-voltage pulse treatment to obtain semen cassiae extract / aloe vera extract / phellodendron amurense extract / honeysuckle flower extract / dandelion extract / centipede extract / forsythia suspense extract / green tea leaf extract.

[0058] Moreover, the electric field strength of the high-voltage pulsed electric field is 40 kV / cm and the frequency is 150 Hz.

[0059] Example 6 The difference between this example and Example 3 lies in the different preparation methods of the semen cassiae extract / aloe vera extract / phellodendron amurense extract / honeysuckle flower extract / dandelion extract / centipede extract / forsythia suspense extract / green tea leaf extract, which are as follows: Step 1: Respectively wash and dry semen cassiae / aloe vera / phellodendron amurense / honeysuckle flower / dandelion / centipede / forsythia suspense / green tea leaves and crush them to between 65 microns to obtain micron-sized semen cassiae powder / micron-sized aloe vera powder / micron-sized phellodendron amurense powder / micron-sized honeysuckle flower powder / micron-sized dandelion powder / micron-sized centipede powder / micron-sized forsythia suspense powder / micron-sized green tea leaf powder; Step 2: After mixing micron-sized semen cassiae powder / micron-sized aloe vera powder / micron-sized phellodendron amurense powder / micron-sized honeysuckle flower powder / micron-sized dandelion powder / micron-sized centipede powder / micron-sized forsythia suspense powder / micron-sized green tea leaf powder with deionized water, form semen cassiae suspension / aloe vera suspension / phellodendron amurense suspension / honeysuckle flower suspension / dandelion suspension / centipede suspension / forsythia suspense suspension / green tea leaf suspension; Step 3: Respectively place the semen cassiae suspension / aloe vera suspension / phellodendron amurense suspension / honeysuckle flower suspension / dandelion suspension / centipede suspension / forsythia suspense suspension / green tea leaf suspension in a high-voltage pulsed electric field and perform high-voltage pulse treatment for 10 - 15 minutes; Step 4: Filter and separate the cassia seed suspension / aloe suspension / phellodendron amurense suspension / honeysuckle suspension / dandelion suspension / centipede suspension / forsythia suspension / green tea suspension after high-voltage pulse treatment, to obtain cassia seed extract / aloe extract / phellodendron amurense extract / honeysuckle extract / dandelion extract / centipede extract / forsythia extract / green tea extract respectively.

[0060] Moreover, the electric field strength of the high-voltage pulsed electric field is 35 kV / cm and the frequency is 125 Hz.

[0061] Comparative Example 1 The difference between this comparative example and Example 3 is that the natural medicine extract does not add ultrafine pearl powder, cassia seed extract, aloe extract, green tea extract, and is the same as the phellodendron amurense liquid preparation; Comparative Example 2 The difference between this comparative example and Example 3 is that the extraction method of the natural medicine extract uses traditional decoction and concentration, without using supercritical carbon dioxide extraction.

[0062] Comparative Example 3 The difference between this comparative example and Example 3 is that the ratio of the aloe extract in the natural medicine extract is different. Specifically: 15 parts of phellodendron amurense extract, 3 parts of ultrafine pearl powder, 2 parts of cassia seed extract, and 15 parts of green tea extract.

[0063] Comparative Example 4 The difference between this comparative example and Example 3 is that the ratio of the aloe extract in the natural medicine extract is different. Specifically: 20 parts of aloe extract, 2 parts of phellodendron amurense extract, 10 parts of ultrafine pearl powder, 13 parts of cassia seed extract, and 3 parts of green tea extract.

[0064] Comparative Example 5 The difference between this comparative example and Example 3 is that the ratio of the aloe extract in the natural medicine extract is different. Specifically: 25 parts of aloe extract, 12 parts of phellodendron amurense extract, 18 parts of ultrafine pearl powder, 13 parts of cassia seed extract, and 15 parts of green tea extract.

[0065] Comparative Example 6 The difference between this comparative example and Example 3 is that the ratio of the aloe extract in the natural medicine extract is different. Specifically: 8 parts of aloe extract, 3 parts of phellodendron amurense extract, 2 parts of ultrafine pearl powder, 2 parts of cassia seed extract, and 2 parts of green tea extract.

[0066] Comparative Example 7 The difference between this comparative example and Example 3 is that the ratio of the bioactive components is different. Specifically: 0.1 part of epidermal growth factor, 4 parts of heparin sodium, 5 parts of mussel adhesive protein, and 0.7 part of nano silver ions.

[0067] Comparative Example 8 The difference between this comparative example and Example 3 lies in: different ratios of bioactive components, specifically: epidermal growth factor is 0.8 parts, the heparin sodium is 1 part, the mussel adhesive protein is 3 parts, and the nano silver ions are 0.8 parts.

[0068] Comparative Example 9 The difference between this comparative example and Example 3 lies in: different ratios of natural medicine extract and bioactive components, specifically: 15 parts of natural medicine extract and 15 parts of bioactive components.

[0069] Comparative Example 10 The difference between this comparative example and Example 3 lies in: different ratios of natural medicine extract and bioactive components, specifically: 35 parts of natural medicine extract and 4 parts of bioactive components.

[0070] Comparative Example 11 The difference between this comparative example and Example 3 lies in: different ratios of natural medicine extract and bioactive components, specifically: 35 parts of natural medicine extract and 15 parts of bioactive components.

[0071] Comparative Example 12 The difference between this comparative example and Example 3 lies in: different ratios of natural medicine extract and bioactive components, specifically: 15 parts of natural medicine extract and 3 parts of bioactive components.

[0072] Experimental Part Experiment 1 1. Animal Preparation Select healthy adult white mice, and use them for experiments after one week of adaptive feeding. Use depilatory cream to depilate the areas on both sides of the spine on the back of the white mice to observe the wounds, and intraperitoneally inject 10% chloral hydrate (400 mg / kg) for anesthesia.

[0073] 2. Wound Model Establishment Use 75% alcohol to disinfect the depilated area, and use a sharp surgical blade to create a linear incision about 1 cm long and about 0.3 cm deep on the disinfected skin. Immediately after the wound is made, gently press it with a sterile gauze to stop bleeding, and then wash the wound with normal saline.

[0074] 3. Grouping and Drug Administration Control Group: Only perform wound cleaning treatment without applying any drugs; Experimental Groups 1 - 12: Comparative Examples 1 - 12; Treatment Group 1: After wound cleaning, apply the hydrogel prepared in Example 3; Treatment Group 3: After wound cleaning, apply the hydrogel prepared in Example 6.

[0075] 4. Wound healing rate On the 1st, 2nd, 3rd, 5th, 7th, and 14th days after wound creation, 5 mice were randomly selected from each group, and the wound healing rate was evaluated using transparent grid paper and weighing method. The results are shown in Table 1.

[0076] Table 1 Wound healing rate Serial number 1d 2d 3d 5d 7d 14d Control group / % 0 0 1.12±0.34 6.12±0.35 10.45±0.38 46.52±4.54 Treatment group 1 / % 2.12±0.65 7.44±1.35 10.88±1.47 48.52±4.15 57.29±4.95 87.18±5.32 Treatment group 2 / % 2.77±0.46 6.89±3.32 12.45±1.94 48.56±5.02 59.54±5.47 88.12±5.23 Experimental group 1 / % 2.13±0.93 4.21±1.35 5.96±1.82 27.85±3.08 46.72±2.82 65.92±3.65 Experimental group 2 / % 0 2.21±1.33 5.98±1.81 28.34±3.21 47.56±3.42 67.45±4.32 Experimental group 3 / % 0 2.33±1.36 5.76±1.21 29.82±3.08 49.72±3.42 68.92±6.67 Experimental group 4 / % 1.12±0.23 5.24±2.35 6.12±1.35 29.43±3.15 46.72±6.56 68.92±4.67 Experimental group 5 / % 1.32±0.45 4.21±1.36 9.96±1.83 28.62±3.06 51.62±3.82 70.92±4.64 Experimental group 6 / % 0 3.23±1.31 6.53±1.74 18.82±3.08 47.72±5.84 68.92±5.67 Experimental group 7 / % 1.17±0.53 5.45±1.45 8.96±2.81 30.62±5.63 59.77±3.86 71.92±4.43 Experimental group 8 / % 1.53±0.67 6.26±1.36 15.95±2.35 29.82±3.08 59.72±3.82 68.92±7.56 Experimental group 9 / % 0 2.33±1.34 5.96±1.82 19.65±2.42 43.76±6.86 57.65±8.11 Experimental group 10 / % 0 2.21±0.35 5.95±1.82 20.82±5.08 39.72±3.82 54.92±4.65 Experimental group 11 / % 0 1.45±1.56 6.96±1.58 21.82±3.54 41.72±3.82 57.92±7.61 Experimental group 12 / % 0 2.79±0.89 5.78±2.88 19.82±3.091 38.72±3.82 52.92±4.67 As can be seen from Table 1, the therapeutic effects of the hydrogels of the present invention (Treatment Groups 1 and 2) are better than those of other experimental groups. At the same time, it was found during the experiment that the wound scars of the mice in Treatment Groups 1 and 2 were lighter than those of the experimental groups and the control group after healing.

[0077] Experiment 2 1. The hydrogels obtained in Examples 3 and 6 and Comparative Examples 1 - 12 were made into circular gel specimens with a diameter of 10 mm and a thickness of 2 mm. 2. Staphylococcus aureus and Escherichia coli were selected and activated in culture until they reached 10 6 CFU / mL. 3. The activated Staphylococcus aureus and Escherichia coli were inoculated onto the circular gel specimens. 4. The inoculated circular gel specimens were placed in an incubator and cultured at 37°C for 24 hours. 5. After the culture was completed, the culture medium was taken out, and the growth of pathogenic microorganisms around the hydrogel samples was observed and recorded. The colony counter was used to count the number of colonies on the culture medium, and the antibacterial rate was calculated. The results are shown in Table 2. As can be seen from Table 2, the hydrogels prepared by the present invention have a significant antibacterial effect compared with the controls.

[0078] Table 2 Antibacterial rate test results Serial number Bacteriostatic rate (%) Example 3 96.3 Example 6 97.5 Comparative example 1 75.6 Comparative example 2 74.5 Comparative example 3 67.4 Comparative example 4 66.5 Comparative example 5 65.8 Comparative example 6 68.7 Comparative example 7 73.5 Comparative example 8 71.1 Comparative example 9 63.3 Comparative example 10 64.1 Comparative example 11 69.6 Comparative example 12 65.2 Experiment 3 1. The hydrogels obtained in Examples 3 and 6 and Comparative Examples 1 - 12 were made into circular gel specimens with a diameter of 10 mm and a thickness of 2 mm, ensuring the same mass. 2. The mass of each sample was accurately weighed using an electronic balance (recorded as M0), and the data was recorded. 3. The weighed samples were carefully placed into a petri dish containing an appropriate amount of deionized water, ensuring that the samples were completely immersed, and soaked for 24 hours. 4. After soaking, the excess water on the surface of the samples was gently blotted with absorbent paper. 5. The mass of the samples was weighed again using an electronic balance (recorded as M1), and the data was recorded.

[0079] 6. The water retention rate was calculated as follows: water retention rate = M0 / (M1 - M0) × 100%. The results are shown in Table 3.

[0080] As can be seen from Table 3, the water hanging rate of the hydrogel prepared by the present invention is higher than that of the comparative example. The hydrogel prepared by the present invention can better provide a wound moisturizing effect, thereby affecting its ability to promote wound healing.

[0081] Table 2 Water holding rate results Serial number Water retention rate (%) Example 3 95.8 Example 6 97.9 Comparative example 1 75.5 Comparative example 2 84.5 Comparative example 3 87.6 Comparative example 4 86.9 Comparative example 5 85.1 Comparative example 6 84.7 Comparative example 7 88.8 Comparative example 8 88.5 Comparative example 9 83.4 Comparative example 10 75.5 Comparative example 11 67.2 Comparative example 12 70.8 In summary, the hydrogel prepared by the present invention not only has a strong wound healing ability, but also has good water holding rate and antibacterial effect.

Claims

1. A hydrogel with wound healing properties, characterized in that, It comprises the following components in parts by mass: 50 - 70 parts of a basic gel matrix, 20 - 30 parts of a natural medicine extract, and 8 - 10 parts of a bioactive ingredient; The natural medicine extract includes ultrafine pearl powder, cassia seed extract, aloe extract, phellodendron bark extract, honeysuckle flower extract, dandelion extract, centipede extract, forsythia extract, and green tea extract; In parts by mass, the ultrafine pearl powder is 8 - 12 parts, the cassia seed extract is 5 - 8 parts, the aloe extract is 15 - 20 parts, the phellodendron bark extract is 6 - 10 parts, the honeysuckle flower extract is 5 - 8 parts, the dandelion extract is 5 - 10 parts, the centipede extract is 1 - 2 parts, the forsythia extract is 5 - 10 parts, and the green tea extract is 5 - 10 parts; The bioactive ingredient includes epidermal growth factor, heparin sodium, mussel adhesive protein, and nano silver ions; In parts by mass, the epidermal growth factor is 0.3 - 0.5 parts, the heparin sodium is 2 - 3 parts, the mussel adhesive protein is 4 - 6 parts, and the nano silver ions are 0.3 - 0.5 parts.

2. The hydrogel with wound healing as described in claim 1, characterized in that, The preparation method of the cassia seed extract / aloe extract / phellodendron bark extract / honeysuckle flower extract / dandelion extract / centipede extract / forsythia extract / green tea extract is as follows: Step 1: Respectively pulverize cassia seeds / aloe / phellodendron bark / honeysuckle flowers / dandelions / centipedes / forsythia / green tea leaves with a pulverizer to obtain cassia seed powder / aloe powder / phellodendron bark powder / honeysuckle flower powder / dandelion powder / centipede powder / forsythia powder / green tea leaf powder; Step 2: Respectively add the cassia seed powder / aloe powder / phellodendron bark powder / honeysuckle flower powder / dandelion powder / centipede powder / forsythia powder / green tea leaf powder to a supercritical extractor; Step 3: Pump carbon dioxide liquid into the supercritical extractor and extract for 90 - 110 min to obtain supercritical carbon dioxide containing cassia seeds / supercritical carbon dioxide containing aloe / supercritical carbon dioxide containing phellodendron bark / supercritical carbon dioxide containing honeysuckle flowers / supercritical carbon dioxide containing dandelions / supercritical carbon dioxide containing centipedes / supercritical carbon dioxide containing forsythia / supercritical carbon dioxide containing green tea respectively; Step 4: Respectively introduce the supercritical carbon dioxide containing cassia seeds / supercritical carbon dioxide containing aloe / supercritical carbon dioxide containing phellodendron bark / supercritical carbon dioxide containing honeysuckle flowers / supercritical carbon dioxide containing dandelions / supercritical carbon dioxide containing centipedes / supercritical carbon dioxide containing forsythia / supercritical carbon dioxide containing green tea into a separator to separately separate out the cassia seed extract / aloe extract / phellodendron bark extract / honeysuckle flower extract / dandelion extract / centipede extract / forsythia extract / green tea extract.

3. A hydrogel with wound healing as claimed in claim 1, wherein, The preparation method of the cassia seed extract / aloe extract / phellodendron bark extract / honeysuckle flower extract / dandelion extract / centipede extract / forsythia extract / green tea extract is as follows: Step 1: After separately washing and drying semen cassiae / aloe barbadensis miller / cortex phellodendri / flos lonicerae / herba taraxaci / scolopendra / folium forsythiae / green tea leaves, crush them to between 50 and 80 microns to obtain micron-level semen cassiae powder / micron-level aloe barbadensis miller powder / micron-level cortex phellodendri powder / micron-level flos lonicerae powder / micron-level herba taraxaci powder / micron-level scolopendra powder / micron-level folium forsythiae powder / micron-level green tea leaves powder; Step 2: Mix the micron-level semen cassiae powder / micron-level aloe barbadensis miller powder / micron-level cortex phellodendri powder / micron-level flos lonicerae powder / micron-level herba taraxaci powder / micron-level scolopendra powder / micron-level folium forsythiae powder / micron-level green tea leaves powder with deionized water to form a semen cassiae suspension / aloe barbadensis miller suspension / cortex phellodendri suspension / flos lonicerae suspension / herba taraxaci suspension / scolopendra suspension / folium forsythiae suspension / green tea suspension; Step 3: Place the semen cassiae suspension / aloe barbadensis miller suspension / cortex phellodendri suspension / flos lonicerae suspension / herba taraxaci suspension / scolopendra suspension / folium forsythiae suspension / green tea suspension in a high-voltage pulsed electric field respectively, and perform high-voltage pulse treatment for 10 - 15 minutes; Step 4: Filter and separate the semen cassiae suspension / aloe barbadensis miller suspension / cortex phellodendri suspension / flos lonicerae suspension / herba taraxaci suspension / scolopendra suspension / folium forsythiae suspension / green tea suspension after high-voltage pulse treatment respectively to obtain semen cassiae extract / aloe barbadensis miller extract / cortex phellodendri extract / flos lonicerae extract / herba taraxaci extract / scolopendra extract / folium forsythiae extract / green tea extract.

4. The hydrogel with wound healing according to claim 3, characterized in that, The electric field strength of the high-voltage pulsed electric field is 30 - 40 kV / cm, and the frequency is 100 - 150 Hz.

5. A hydrogel with wound healing as claimed in claim 1, wherein, The particle size of the ultrafine pearl powder is less than or equal to 10 microns.

6. A hydrogel with wound healing as described in claim 5, characterized in that, The basic gel matrix includes sodium hyaluronate, sodium alginate, collagen, polyacrylamide, polyvinyl alcohol, carbomer, nano calcium alginate; By mass, the sodium hyaluronate is 5 - 10 parts, the sodium alginate is 10 - 20 parts, the collagen is 10 - 15 parts, the polyacrylamide is 5 - 8 parts, the polyvinyl alcohol is 10 - 15 parts, the carbomer is 2 - 3 parts, and the nano calcium alginate is 3 - 5 parts.

7. A hydrogel having wound healing as described in claim 6, wherein The preparation method of the hydrogel for wound healing is as follows: S1: Take sodium hyaluronate, sodium alginate, collagen, polyacrylamide, polyvinyl alcohol, carbomer, dissolve each of them in deionized water, heat and continuously stir to make them dissolve evenly to form their respective matrix solutions; S2: Uniformly disperse nano calcium alginate in deionized water to form a nano calcium alginate suspension; S3: Mix the matrix solution and the nano calcium alginate suspension, heat and continuously stir until a uniform basic gel matrix is formed; S4: Take ultrafine pearl powder, semen cassiae extract, aloe barbadensis miller extract, cortex phellodendri extract, flos lonicerae extract, herba taraxaci extract, scolopendra extract, folium forsythiae extract, green tea extract, disperse each of them in deionized water to form their respective natural medicine extract solutions; S5: Add the natural medicine extract solution prepared in step S4 to the basic gel matrix prepared in step S3, heat and continuously stir to make them mix evenly to obtain a pretreated gel; S6. Dissolve epidermal growth factor, sodium heparin, and mussel adhesive protein in physiological saline respectively to form their respective bioactive ingredient solutions; S7. Add silver nanoparticles into physiological saline to make it evenly dispersed, forming a silver nanoparticle suspension; S8. Add the bioactive ingredient solutions prepared in step S6 and the silver nanoparticle suspension prepared in step S7 into the pre-treated gel prepared in step S5, heat and continuously stir to make them evenly mixed, obtaining a hydrogel for wound healing.

8. A hydrogel having wound healing as described in claim 7, wherein, In steps S1, S3, S5, and S8, the stirring speed is 200 - 400 rpm / min, and the heating temperature is 30 - 35 °C.

9. A hydrogel with wound healing as described in claim 8, characterized in that, In step S1, the material-liquid ratios of sodium hyaluronate, sodium alginate, collagen, polyacrylamide, polyvinyl alcohol, carbomer to ionized water are 1:4 - 6, 1:10 - 13, 1:10 - 12, 1:6 - 7, 1:2 - 3, 1:2 - 3 respectively; In step S2, the material-liquid ratio of nano calcium alginate to ionized water is 1:4 - 6; In step S4, the ratios of ultrafine pearl powder, cassia seed extract, aloe extract, phellodendron bark extract, honeysuckle flower extract, dandelion extract, centipede extract, forsythia extract, green tea extract to deionized water are 1 - 2:10, 1 - 2:10, 1 - 2:10, 1 - 2:10, 1 - 2:10, 1 - 2:10, 1 - 2:20, 1 - 2:10, 1 - 2:

10.

10. A hydrogel with wound healing as described in claim 8, characterized in that, In step S6, the material-liquid ratios of epidermal growth factor, sodium heparin, mussel adhesive protein to physiological saline are 1 - 2:10, 2 - 3:10, 2 - 3:10 respectively; In step S7, the material-liquid ratio of silver nanoparticles to physiological saline is 1 - 2:10.