Nanoscale impurity removal method and system for blood products

Abstract

The invention belongs to the technical field of impurity removal of blood products, and discloses a nanoscale impurity removal method for blood products, the removal precision of the nanoscale impurity removal method is remarkably improved, a magnetic control nano adsorbent is used for specifically adsorbing nano impurities smaller than 100 nm in a high-selectivity mode, the removal rate of impurity particles in the treated blood products reaches 95% or above, and the removal rate of the impurity particles in the treated blood products reaches 95% or above. The purity of a blood product is obviously improved. According to the invention, the activity loss caused by the mechanical shearing or pressure difference effect of the traditional membrane filtration technology and centrifugal sedimentation on blood product proteins, active factors and cells is avoided. Through mild magnetic adsorption and magnetic field separation processes, mechanical damage to active components of the product is reduced, the retention rate of active substances reaches 95% or above, and the problem of damage to the active components in the prior art is effectively solved.

Description

Technical Field

[0001] The present invention belongs to the technical field of impurity removal from blood products, and particularly relates to a method and system for removing nanoscale impurities from blood products. Background Art

[0002] Prior Art 1: Traditional membrane filtration technology

[0003] In the existing blood product processing, ultrafiltration membranes or nanofiltration membranes are generally used to filter impurities, which can effectively retain proteins and viruses, but have poor removal effect on nanoscale impurities with a particle size less than 50 nm, and are prone to membrane fouling, resulting in low filtration efficiency and short membrane service life. At the same time, the membrane technology is also prone to non-specific adsorption of proteins, causing loss of target components and membrane fouling, increasing the cleaning cost and reducing the separation efficiency.

[0004] Prior Art 2: Centrifugal sedimentation method

[0005] Using centrifugation technology to sediment and separate blood products can effectively remove impurities within a certain particle size range, but has limited removal effect on nanoscale impurities less than 100 nm. Moreover, due to the high shear force generated during centrifugation, it is easy to damage active substances such as proteins, resulting in reduced biological activity. In addition, the centrifugation process has complex equipment, high cost, complex operation, and poor process stability, making it difficult to meet the requirements of precise and efficient impurity removal.

[0006] Therefore, the problems that need to be solved urgently in the above prior art are: for nanoscale impurities (less than 100 nm) in blood products, develop a highly efficient, stable, and selective removal method and system, and achieve high-purity products while ensuring the activity of the effective components of blood products.

[0007] Through the above analysis, the problems and defects existing in the prior art are:

[0008] (1) In the existing blood product processing, ultrafiltration membranes or nanofiltration membranes are generally used to filter impurities, which can effectively retain proteins and viruses, but have poor removal effect on nanoscale impurities with a particle size less than 50 nm, and are prone to membrane fouling, resulting in low filtration efficiency and short membrane service life. At the same time, the membrane technology is also prone to non-specific adsorption of proteins, causing loss of target components and membrane fouling, increasing the cleaning cost and reducing the separation efficiency.

[0009] (2) The use of centrifugation technology to sediment and separate blood products can effectively remove impurities within a certain particle size range, but has limited effectiveness in removing nanoscale impurities smaller than 100 nm. Moreover, due to the high shear forces generated during centrifugation, it is easy to damage active substances such as proteins, resulting in a reduction in biological activity. In addition, the equipment for the centrifugation process is complex, costly, the operation is complex, and the process stability is poor, making it difficult to achieve the requirements of precise and efficient impurity removal. Summary of the Invention[[ID={3}]]

[0010] In view of the problems existing in the prior art, the present invention provides a method for removing nanoscale impurities from blood products.

[0011] The present invention is implemented as follows. A method for removing nanoscale impurities from blood products includes:

[0012] Step 1, functionalization of magnetic nanoparticles;

[0013] Step 2, pretreatment of blood products;

[0014] Step 3, magnetic field-assisted rapid separation;

[0015] Step 4, on-line real-time detection and intelligent feedback control. [[ID={21}]]

[0016] Furthermore, for the functionalization of magnetic nanoparticles:

[0017] By using magnetic iron oxide nanoparticles with their surfaces modified with amino groups, and then coupling antibodies specifically recognizing impurities to the particle surfaces via cross-linking agents, magnetic nano-adsorbents with high selectivity are obtained.

[0018] Furthermore, for the pretreatment of blood products:

[0019] The blood products are continuously flowed into a nano-adsorption column through a microfluidic chip at a flow rate of 1 mL / min. Under the assistance of a magnetic control field, the magnetic nanoparticles are uniformly dispersed in the blood, fully contacting and specifically adsorbing the nano-impurities.

[0020] Furthermore, for the step of magnetic field-assisted rapid separation:

[0021] The blood products after adsorption treatment enter the magnetic separation area. By applying a magnetic field gradient (0.1 - 0.5 T / m), the magnetic particles are rapidly enriched and separated under the action of the magnetic field.

[0022] Furthermore, for the on-line real-time detection and intelligent feedback control:

[0023] The real-time monitoring system detects changes in the particle size of impurities through dynamic light scattering. If the number of particles with a particle size smaller than 100 nm detected does not reach the set value, the system automatically feeds back to the adsorption and magnetic field control modules, automatically adjusting the magnetic field strength and treatment time until the best purification effect is achieved.

[0024] Another object of the present invention is to provide a nanoscale impurity removal system for blood products, comprising:

[0025] Magnetic nanosorption module: By means of magnetic control, nanoscale magnetic particles are uniformly arranged, and the surfaces of the magnetic particles are functionalized with hydrophilic ligands or specific recognition antibodies, which can selectively adsorb nanoscale impurities in blood products, including lipid microparticles, endotoxins, and small-sized virus impurities;

[0026] Magnetic field control and separation module: Comprising an adjustable permanent magnet array and a magnetic field generator, with the magnetic field intensity adjustable in the range of 0.1 - 2 Tesla;

[0027] Dynamic fluid control module: The flow rate is precisely regulated by an intelligent microfluidic system, accurately controlling the liquid flow velocity and shear force;

[0028] Real-time monitoring and intelligent feedback control module: Equipped with high-precision laser dynamic light scattering instruments, fluorescence microscopes, and ultraviolet spectrometers, which can detect the particle size distribution, concentration, and compositional changes of impurities in blood products before and after treatment in real time, and feedback signals to the artificial intelligence analysis module; After the AI algorithm analyzes the monitoring results, it optimizes the magnetic field intensity, particle dispersion density, and flow rate parameters.

[0029] Combined with the above technical solutions and the technical problems solved, the advantages and positive effects of the technical solution to be protected by the present invention are as follows:

[0030] Traditional membrane filtration and centrifugal sedimentation technologies are difficult to effectively remove nanoscale impurities smaller than 100 nm. The present invention uses magnetically controlled nanosorbents to specifically adsorb nanoscale impurities smaller than 100 nm with high selectivity. The removal rate of impurity particles in the treated blood products reaches more than 95%, significantly improving the purity of blood products.

[0031] The present invention avoids the activity loss caused by the mechanical shearing or pressure difference of traditional membrane filtration technology and centrifugal sedimentation on proteins, active factors, and cells in blood products. Through a mild magnetic adsorption and magnetic field separation process, the mechanical damage to the active ingredients of the product is reduced, and the retention rate of active substances reaches more than 95%, effectively solving the problem of damage to active ingredients in the prior art.

[0032] The present invention detects the distribution and quantity of impurity particles in real time through dynamic laser scattering, automatically optimizes the magnetic field intensity, magnetic particle concentration, and treatment flow rate based on intelligent algorithms, constitutes a closed-loop automated precision control mode, greatly reduces the frequency of human intervention, realizes the intelligentization and automation of the treatment process, and improves the treatment efficiency by more than 30%.

[0033] Compared with the prior art, the present invention does not require frequent replacement of the filter membrane, does not require frequent cleaning of the filter, has no obvious blockage risk, greatly simplifies the maintenance process, reduces the production cost, and improves the processing efficiency of blood products.

[0034] Aiming at the problem of removing nano-scale impurities in blood products, the present invention realizes the efficient removal of impurities through an efficient selective magnetic nano-adsorption technology and an intelligent feedback control system, while ensuring the purity and activity of blood products, and has obvious technical advantages and significant technological progress. BRIEF DESCRIPTION OF THE DRAWINGS

[0035] Figure 1 is a flowchart of a method for removing nano-scale impurities in blood products provided by an embodiment of the present invention.

[0036] Figure 2 is a flowchart of a magnetic field-assisted rapid separation method provided by an embodiment of the present invention.

[0037] Figure 3 is a block diagram of a system structure for removing nano-scale impurities in blood products provided by an embodiment of the present invention. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0038] In order to make the objectives, technical solutions and advantages of the present invention clearer and more understandable, the present invention will be further described in detail below with reference to the embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0039] As Figure 1 shown, the method for removing nano-scale impurities in blood products provided by an embodiment of the present invention includes the following steps:

[0040] S101, functionalization of magnetic nanoparticles;

[0041] S102, pretreatment of blood products;

[0042] S103, magnetic field-assisted rapid separation;

[0043] S104, on-line real-time detection and intelligent feedback control.

[0044] In the embodiments of the present invention, nano-ferroferric oxide (Fe3O4) magnetic particles are used as the core material, and high reactivity is imparted to them by surface modification with amino groups (-NH2). Subsequently, a crosslinking agent (such as glutaraldehyde, EDC / NHS system) is used to couple specific recognition antibodies on the particle surface, enabling the magnetic nanoparticles to have high-affinity adsorption for target impurities (such as viruses, protein aggregates or cell debris) in blood products. This functionalization process enhances the selectivity, biocompatibility and stability of the magnetic nanoparticles, ensuring the ability to efficiently remove nano-scale impurities during the actual separation process while avoiding damage to the blood active components.

[0045] In the pretreatment of blood products, a microfluidic chip system is used to continuously pump the blood product into the nano-adsorption column at a constant flow rate of 1 mL / min. The laminar flow environment provided by the microfluidic system can significantly improve the dispersion uniformity of the magnetic nanoparticles, ensuring a high specific surface area in the blood sample, thereby increasing the effective capture probability of impurity molecules.

[0046] In addition, under the action of an externally applied magnetic control field (such as an alternating magnetic field or a gradient magnetic field), the magnetic nanoparticles exhibit an enhanced Brownian motion effect, further promoting their full contact and dynamic binding with nano-impurities in the blood, maximizing the adsorption efficiency. This process not only improves the impurity removal effect but also effectively reduces the aggregation risk of the magnetic nanoparticles, ensuring the stable operation of the system.

[0047] When the magnetic nanoparticles complete the specific adsorption of blood impurities, a high-gradient magnetic field (High-Gradient Magnetic Field, HGMF) is applied for magnetic field-assisted rapid separation. Specifically, under the drive of the magnetic field, the magnetic nanoparticles carrying impurities rapidly converge along the magnetic field gradient direction and form a high-density deposition area in the nano-adsorption column, allowing the clean blood components to flow out from the system outlet.

[0048] Compared with traditional centrifugation or membrane separation methods, the magnetic field-assisted separation technology of the present invention has the advantages of being non-destructive, high-speed and continuously processable, which can significantly reduce blood component loss while ensuring that the separated blood products still have high biological activity. In addition, this method shows good removal ability for different types of blood impurities, including viruses, protein aggregates and microparticle pollutants, further improving the purity and safety of blood products.

[0049] The embodiments of the present invention are equipped with an on-line real-time detection system, combined with an intelligent feedback control algorithm, to ensure the monitorability and automatic optimization ability of the impurity removal process. The on-line detection module uses optical transmission method, dynamic light scattering (DLS) or nanoparticle tracking analysis (NTA) to measure the concentration of residual impurities in the blood product in real time and dynamically evaluate the adsorption process through a data processing algorithm.

[0050] The intelligent feedback system can adaptively adjust the parameters of the magnetic control field, the flow rate of the microfluidic chip, or the nanoparticle concentration of the adsorption column according to the real-time data of impurity removal efficiency, blood flow rate, and magnetic field strength, so as to optimize the separation efficiency and ensure that the quality of the final product meets the clinical-grade standards.

[0051] To improve the sustainability of the system, the embodiments of the present invention have designed the nano-adsorption column to be renewable. After the magnetic nanoparticles inside the adsorption column complete the impurity removal, desorption and regeneration can be achieved through methods such as weak acid elution, ultrasonic oscillation, or high-salt ion competitive elution. The eluted magnetic nanoparticles can further use ultrasonic fragmentation or dynamic recombination technology to re-expose the antibodies on their surfaces, ensuring the high efficiency of the next round of separation.

[0052] In addition, the stability optimization of the system is reflected in:

[0053] A low shear force environment reduces the risk of blood protein denaturation;

[0054] Constant temperature control (such as 37°C) prevents the degradation of blood components;

[0055] Optimized design of magnetic field uniformity to avoid the influence of local magnetic aggregation on the adsorption effect.

[0056] These optimization measures significantly improve the stability of the system during long-term operation and ensure the high quality and high safety of blood products.

[0057] The magnetic nanoparticle functionalization adsorption technology proposed in the embodiments of the present invention combines a microfluidic chip, magnetic field-assisted separation, and intelligent feedback control to construct a high-efficiency, controllable, and low-loss blood product impurity removal system. The core technical advantages include:

[0058] Ultra-high selectivity: Through antibody functionalization, precise removal of specific impurities is achieved;

[0059] Fast separation ability: Magnetic field assistance accelerates impurity clearance, reduces blood residence time, and ensures the stability of active ingredients;

[0060] Online intelligent optimization: Using real-time detection and intelligent feedback control to achieve full-process automatic adjustment and improve the adaptability of the system;

[0061] Sustainable operation: The nano-adsorption column can be recycled, reducing operating costs and improving the durability of the system.

[0062] This method has broad application potential in the fields of blood product production, virus removal, biopharmaceutical purification, and precision medicine, providing a new and efficient technical solution for the preparation and clinical application of high-end blood products.

[0063] Functionalization of magnetic nanoparticles provided by the embodiments of the present invention:

[0064] The surface of nano-sized magnetite magnetic particles is modified with amino groups, and then an antibody specifically recognizing impurities is conjugated to the particle surface via a cross-linking agent to obtain a magnetic nano-adsorbent with high selectivity.

[0065] Pretreatment of blood products provided by the embodiments of the present invention:

[0066] The blood product is continuously flowed into the nano-adsorption column through the microfluidic chip at a flow rate of 1 mL / min. Under the assistance of a magnetic control field, the magnetic nanoparticles are uniformly dispersed in the blood, fully contacting and specifically adsorbing the nano-impurities.

[0067] As Figure 2 shown, the magnetic field-assisted rapid separation step provided by the embodiments of the present invention:

[0068] S201, the adsorbed blood product enters the magnetic separation area. By applying a magnetic field gradient (0.1 - 0.5 T / m), the magnetic particles are rapidly enriched and separated under the action of the magnetic field.

[0069] Online real-time detection and intelligent feedback control provided by the embodiments of the present invention:

[0070] The real-time monitoring system detects the change in the particle size of impurities through dynamic light scattering. If the number of particles with a particle size less than 100 nm detected does not reach the set value, the system automatically feeds back to the adsorption and magnetic field control module to automatically adjust the magnetic field strength and processing time until the best purification effect is achieved.

[0071] As shown in Figure 3, a nano-scale impurity removal system for blood products provided by the embodiments of the present invention includes:

[0072] Magnetic nano-adsorption module: By means of magnetic control, nano-scale magnetic particles are uniformly arranged. The surface of the magnetic particles is functionally modified with hydrophilic ligands or specifically recognizing antibodies, which can selectively adsorb nano-scale impurities in blood products, including lipid microparticles, endotoxins, and virus small particle size impurities;

[0073] Magnetic field control and separation module: It includes an adjustable permanent magnet array and a magnetic field generator, and the magnetic field strength adjustment range is 0.1 - 2 Tesla;

[0074] Dynamic fluid control module: The flow rate is precisely regulated by an intelligent microfluidic system to accurately control the liquid flow velocity and shear force;

[0075] Real-time monitoring and intelligent feedback control module: Equipped with a high-precision laser dynamic light scattering instrument, a fluorescence microscope, and an ultraviolet spectrometer, it can detect the impurity particle size distribution, concentration, and composition changes in blood products before and after processing in real time, and feedback signals to the artificial intelligence analysis module; after the AI algorithm analyzes the monitoring results, it optimizes the magnetic field strength, particle dispersion density, and flow rate parameters.

[0076] The present invention proposes a blood product nano-scale impurity removal system based on the synergistic technology of microfluidic magnetic nano-impurity adsorption - magnetic separation. The system structure is as follows:

[0077] (1) Magnetic nano-adsorption module:

[0078] By means of magnetic control, nano-scale magnetic particles (particle size 20 - 50 nm) are uniformly arranged. The surface of the magnetic particles is functionalized with hydrophilic ligands or specific recognition antibodies, which can selectively adsorb nano-scale impurities in blood products, including small particle size impurities such as lipid microparticles, endotoxins, and viruses.

[0079] (2) Magnetic field control and separation module:

[0080] It includes an adjustable permanent magnet array and a magnetic field generator. The magnetic field strength can be adjusted in the range of 0.1 - 2 Tesla, which can accurately control the magnetic field strength and gradient, realize the rapid and uniform dispersion and rapid aggregation of magnetic particles, and thus precisely control the impurity adsorption and removal process.

[0081] (3) Dynamic fluid control module:

[0082] The flow rate (0.1 - 2 mL / min) is precisely regulated by an intelligent microfluidic system (such as a micro peristaltic pump), accurately controlling the liquid flow velocity and shear force to ensure sufficient and efficient contact between magnetic particles and nano-impurities, and improving the adsorption efficiency and separation rate.

[0083] (4) Real-time monitoring and intelligent feedback control module:

[0084] Equipped with a high-precision laser dynamic light scattering (DLS) instrument, a fluorescence microscope, and an ultraviolet spectrometer, it can detect the impurity particle size distribution, concentration, and composition changes in blood products before and after processing in real time, and feedback signals to the artificial intelligence analysis module. After the AI algorithm analyzes the monitoring results, it optimizes parameters such as the magnetic field strength, particle dispersion density, and flow rate to achieve precise regulation and efficient impurity removal.

[0085] III. Specific method steps of the present invention

[0086] (1) Functionalization of magnetic nanoparticles:

[0087] Using magnetic iron oxide nanoparticles (particle size 30 nm) with amino groups modified on the surface, and then coupling antibodies specifically recognizing impurities on the particle surface through a cross-linking agent to obtain a highly selective magnetic nano-adsorbent.

[0088] (2) Pretreatment of blood products:

[0089] The blood products are continuously flowed into the nano-adsorption column through the microfluidic chip at a flow rate of 1 mL / min. Under the assistance of a magnetic control field, the magnetic nanoparticles are uniformly dispersed in the blood, fully contacting and specifically adsorbing with the nano-impurities.

[0090] (3) Magnetic field-assisted rapid separation step:

[0091] The adsorbed blood products enter the magnetic separation area. By applying a magnetic field gradient (0.1 - 0.5 T / m), the magnetic particles are rapidly enriched and separated under the action of the magnetic field, thus realizing the efficient separation of impurities from pure blood products. The separation time is less than 10 minutes, and the separation efficiency is increased by more than 50%.

[0092] (4) On-line real-time detection and intelligent feedback control:

[0093] The real-time monitoring system detects the change of impurity particle size through dynamic light scattering. If the number of particles with an impurity particle size less than 100 nm detected does not reach the set value, the system automatically feeds back to the adsorption and magnetic field control module, and automatically adjusts the magnetic field strength and treatment time until the best purification effect is achieved.

[0094] IV. The specific method steps are as follows:

[0095] (1) After the blood products are pretreated, they are transported to the adsorption reaction module through the injection system, and a complex is formed by the reaction of the magnetic nano-adsorbent with impurities; (2) The microfluidic device is used to control the sample flow rate (flow rate 0.1 - 0.3 mL / min), and under specific magnetic field conditions (magnetic field strength 0.1 - 0.5 T / m), magnetic adsorption treatment is carried out, and the impurity particles are rapidly adsorbed onto the surface of the magnetic particles; (3) The treated mixed liquid enters the magnetic separation device. Under the gradient action of the magnetic field, the magnetic particles adsorbed with impurities are rapidly enriched at one end of the magnetic pole. After removing the magnetic particles by filtration, the blood products continue to enter the subsequent ultrafiltration or dialysis system for further treatment; (4) The DLS dynamic light scattering system and fluorescence microscope are used to real-time monitor the treatment effect. If there are still impurities below the target particle size, the intelligent feedback control system automatically repeats the adsorption and separation steps until the purification target is achieved.

[0096] Example 1:

[0097] Magnetic Fe3O4 nanoparticles (particle size 30 nm) were taken, and the surface was aminated by APTES. Then, they were covalently coupled with specific antibodies against target impurities through glutaraldehyde crosslinker to prepare a functionalized magnetic nano-adsorbent for standby. The blood sample was fed into the nano-adsorption column by a microfluidic pump at a flow rate of 0.2 mL / min. Under the action of a uniform magnetic field of 0.3 T, the nanoparticles were fully dispersed and came into full contact with the nano-impurities in the blood, and the adsorption time was about 10 minutes. The adsorbed blood entered the magnetic separation area, and the magnetic field was quickly switched to a magnetic field gradient of 0.5 T / m. The magnetic particles were enriched and settled to the bottom of the separation area within 2 minutes. After removing the magnetic particles through a filter screen, the blood continued to enter the subsequent ultrafiltration device for treatment. The dynamic light scattering instrument and fluorescence microscope were used for real-time detection, and the particle size of the nano-impurities was significantly reduced, and the average removal rate reached more than 60%.

[0098] Example 2:

[0099] Magnetic Fe3O4 nanoparticles with surface amino modification (particle size 40 nm) were weighed and prepared into particle adsorbents for standby after glutaraldehyde crosslinking treatment. The blood sample entered the nano-adsorption column through the microfluidic device at a flow rate of 0.15 mL / min. The magnetic field intensity was set to 0.2 T. After continuous adsorption for 15 minutes, the magnetic field was adjusted to 0.4 T for rapid aggregation separation. After removing the magnetic particles with a filter membrane, the blood entered the subsequent dialysis system for treatment. At the same time, the dynamic light scattering instrument monitored the particle size distribution of the impurities in real time, and the removal efficiency of nano-scale impurities (particle size less than 100 nm) after treatment reached more than 55%.

[0100] As mentioned above, it is only the specific implementation manners of the present invention, but the protection scope of the present invention is not limited thereto. Any person skilled in the art within the technical scope disclosed by the present invention, any modifications, equivalent substitutions, and improvements made within the spirit and principle of the present invention should be covered within the protection scope of the present invention.

Claims

1. A method for removing nanoscale impurities from blood products, characterized in that, It includes the following steps: 1) Functionalization of magnetic nanoparticles: Modify the surface of magnetic nanoparticles of nano-ferroferric oxide (Fe3O4) with amino groups, and couple specific recognition antibodies through a cross-linking agent; 2) Transport of blood products: Transport the blood products through the microfluidic chip to the nano-adsorption column at a set flow rate; 3) Magnetic field action: In a high-gradient magnetic field environment, make the magnetic nanoparticles fully contact with the blood sample; 4) Impurity adsorption: Achieve the capture of target impurities through the specific binding of antibodies on the surface of magnetic nanoparticles; 5) Magnetic field separation: Under the drive of the magnetic field, enrich the magnetic nanoparticles carrying impurities and separate them from the blood products; 6) Online detection: Monitor the impurity concentration through optical transmission detection, dynamic light scattering (DLS) or nanoparticle tracking analysis (NTA); 7) Feedback control: Adjust the magnetic field strength, blood flow rate and magnetic nanoparticle concentration according to the online detection results.

2. The method according to claim 1, characterized in that During the functionalization process of the magnetic nanoparticles, the modification of the amino group adopts silanization treatment, and the cross-linking agent is selected from glutaraldehyde or the EDC / NHS system; the channel width of the microfluidic chip during the transport process of the blood products is 10 - 200 μm, and the flow rate is 0.1 - 5 mL / min.

3. The method according to claim 1, wherein In the magnetic field action step, the magnetic field strength is 0.1 - 2 T, and the magnetic field gradient range is 10 - 500 T / m; during the magnetic field separation process, a magnetic field gradient distribution area is provided in the nano-adsorption column to form a multi-stage magnetic enrichment area.

4. The method according to claim 1, wherein During the feedback control process, the impurity concentration data measured based on dynamic light scattering (DLS) or nanoparticle tracking analysis (NTA) is used, and the magnetic field strength, blood flow rate and nanoparticle concentration are adjusted in combination with an adaptive control algorithm.

5. The nanoscale impurity removal method for blood products according to claim 1, characterized in that, The pretreatment of the blood products: The blood products are continuously flowed into the nano-adsorption column through the microfluidic chip at a flow rate of 1 mL / min. Under the assistance of the magnetic control field, the magnetic nanoparticles are uniformly dispersed in the blood, fully contact with the nano-impurities and specifically adsorb them.

6. The nano-level impurity removal method for blood products according to claim 1, characterized in that, The magnetic field-assisted rapid separation step: The blood products treated by adsorption enter the magnetic separation area. By applying a magnetic field gradient (0.1 - 0.5 T / m), the magnetic particles are rapidly enriched and separated under the action of the magnetic field.

7. The nano-level impurity removal method for blood products according to claim 1, wherein The online real-time detection and intelligent feedback control: The real-time monitoring system detects the change of impurity particle size through dynamic light scattering. If the number of particles with an impurity particle size less than 100 nm detected does not reach the set value, the system automatically feeds back to the adsorption and magnetic field control module, and automatically adjusts the magnetic field strength and treatment time until the best purification effect is achieved.

8. A nanoscale impurity removal system for blood products that implements the nanoscale impurity removal method for blood products described in any one of claims 1-7, characterized in that, The nano-level impurity removal system for blood products includes: Magnetic nano-adsorption module: Uniformly arrange nano-level magnetic particles in a magnetic control manner. The surface of the magnetic particles is functionally modified with hydrophilic ligands or specific recognition antibodies, and can selectively adsorb nano-level impurities in blood products, including lipid microparticles, endotoxins and virus small-particle impurities; Magnetic field control and separation module: Includes an adjustable permanent magnet array and a magnetic field generator, and the magnetic field strength adjustment range is 0.1 - 2 Tesla; Dynamic fluid control module: The flow rate is precisely regulated by an intelligent microfluidic system, and the liquid flow rate and shear force are precisely controlled; Real-time monitoring and intelligent feedback control module: Equipped with high-precision laser dynamic light scattering instrument, fluorescence microscope and ultraviolet spectrometer, it can detect the impurity particle size distribution, concentration and composition changes before and after the blood product treatment in real time, and feedback signals to the artificial intelligence analysis module; After analyzing the monitoring results with AI algorithms, the magnetic field strength, particle dispersion density and flow rate parameters are optimized.

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