Flow cytometry antibody premixing device

Abstract

The invention relates to the technical field of cell detection, in particular to a flow cytometry antibody premixing device. Comprising a box body, the box body is provided with a cavity, an elastic movable part extends into the cavity to be connected with the box body, two notch gaps are formed in the side wall of the elastic movable part to form two movable petals, and when no external force extrusion exists, the two movable petals tend to the notch gaps to extend and be attached to the notch gaps so that a closed space can be formed in the cavity below the movable petals; the annular body is installed on the inner wall of the elastic movable part, and when the annular body is extruded by external force, the annular body deforms so that a closed space can be formed in a cavity below the annular body; the EP tube is mounted in the chamber and is used for premixing flow cytometry antibodies. The EP tube in the box body can be ensured to be always in a closed state isolated from the outside in the antibody mixture preparation process, so that the contact with the outside is reduced, and the pollution risk of the antibody mixture is reduced.

Description

Technical Field

[0001] The present invention relates to the technical field of cell detection, and more particularly to a flow cytometry antibody premixing device. Background Art

[0002] Flow cytometry is a single-cell quantitative analysis and sorting technology using a flow cytometer, which can perform multi-parameter high-speed analysis and sorting on small particulate matters such as cells and microorganisms, and has the characteristics of being quantifiable, flexible, and fast. It is one of the most advanced cell quantitative analysis technologies at present.

[0003] Some specific reagents are very important for flow cytometry technology, such as fluorescent dye-labeled antibodies against specific cell antigens. Testers need to optimize each protocol to increase accuracy and reduce errors and differences, and finally determine the best available antibodies, fluorophores, and their combinations. Antibody mixtures are very useful tools in clinical process laboratories, which contribute to the consistency of detection. An antibody mixture is a combination of individual antibodies for analysis in a single tube. Compared with direct purchase, preparing an antibody mixture by a tester has the advantages of low cost and flexible customization. When preparing an antibody mixture, each monoclonal antibody must be titrated separately to achieve the best signal-to-noise ratio separation.

[0004] When there are many antibodies to be mixed for preparing an antibody mixture, the EP tube for preparing the antibody mixture will remain continuously open. When the tester is performing the preparation operation, the continuously open state of the EP tube will increase the risk of contamination of the antibody mixture. Summary of the Invention

[0005] The present invention aims to at least improve one of the technical problems existing in the prior art. To this end, the present invention provides a flow cytometry antibody premixing device.

[0006] The technical solution of the present invention is as follows: A flow cytometry antibody premixing device, comprising: A box body having a chamber, and a through hole is provided on the surface of the box body; An elastic movable member passing through the through hole and extending into the chamber, the elastic movable member being connected to the surface of the box body. The diameter of the elastic movable member away from the surface of the box body gradually contracts, for a pipette gun to pass through and extend into the chamber. Two notch slits are provided on the side wall of the elastic movable member to form two movable flaps. When there is no external force extrusion, the two movable flaps tend to extend and fit towards the notch slits so that the chamber below the movable flaps forms a sealed space; A ring body installed on the inner wall of the elastic movable member and located above the movable flaps. When externally extruded, the ring body deforms so that the chamber below the ring body forms a sealed space; An EP tube is installed in the chamber for pre-mixing flow cytometry antibodies.

[0007] In a possible technical solution, further, the ring body includes: A plurality of limiting strips are annularly and spacedly distributed, and there is a gap between adjacent limiting strips; A sealing belt, and each sealing belt passes through the gap and is respectively connected to adjacent two limiting strips to form an annular closed loop, so that when being extruded by an external force, the ring body deforms to form a sealed space in the chamber below the ring body.

[0008] In a possible technical solution, further, the sealing belt has an arc-shaped contour deviating from the limiting strip. When being extruded by an external force, the sealing belt stretches in the direction close to the limiting strip to increase the area of the region surrounded by the ring body, providing a displacement space for the pipette.

[0009] In a possible technical solution, further, the ring body further includes: A clamping member, one end of which is connected to the side wall of the limiting strip deviating from the elastic movable member.

[0010] In a possible technical solution, further, the sealing belt has a first side arc and a second side arc, wherein the first side arc is the inner side arc close to the surface of the limiting strip, and the second side arc is the outer side arc deviating from the surface of the limiting strip, and the arc length of the first side arc is greater than the arc length of the second side arc.

[0011] In a possible technical solution, further, the end of the clamping member away from the limiting strip approaches the inner wall of the elastic movable member. The clamping member and the upper surface of the limiting strip and the inner wall of the elastic movable member enclose a clamping gap for the sealing belt to pass through, improving the overall tightness of the device, wherein the clamping member is used to increase the limiting effect of the clamping gap.

[0012] In a possible technical solution, further, the sealing belt is made of an elastic material, which is convenient for being expanded under pressure to seal the gap.

[0013] In a possible technical solution, further, the two movable flaps are symmetrically distributed, which is convenient for evenly expanding outward when being pressed to ensure that the pipette moves vertically downward, avoiding the pipette touching the movable flap due to asymmetric deformation.

[0014] In a possible technical solution, further, a groove is provided on the inner side of the box body for fixing the EP tube.

[0015] In a possible technical solution, further, a visual glass is provided on any side surface of the box body for easy observation.

[0016] According to the flow cytometry antibody premixing device of the present invention, it can ensure that the EP tube containing the antibody mixture in the box is in a closed state isolated from the outside world throughout the entire process before and after the antibody is configured, reducing contact with the outside world and lowering the risk of antibody mixture contamination. Specifically, it is reflected in: By providing at least two notches at the lower end of the elastic movable member to form at least two movable flaps, when the pipette enters the elastic movable member, a supporting force is generated between the surface of the pipette tip ejector and the inner wall of the limiting strip, and the movable flaps are pushed outwards, facilitating the entry of the pipette tip ejector and the disposable tip cone into the chamber of the box. Moreover, due to the limiting effect of the clamping member on the sealing strip, the sealing strip maintains a tight closed fan-shaped gap state during the process of passing through the fan-shaped gap between the limiting strips to isolate contact with the outside world; During the process of the lower end of the movable flap opening, the lower end of the cavity of the elastic movable member surrounded by the movable flap communicates with the chamber of the box, enabling the overall formed by at least two limiting strips to be a complete ring under the penetration of the sealing strip, facilitating separation from the upper end of the cavity of the elastic movable member, ensuring the sealing performance of the entire pipette during the process of entering the device when mixing antibodies, and reducing the risk of antibody mixture contamination caused by the continuous opening of the EP tube.

[0017] The additional aspects and advantages of the present invention will be partially given in the following description, partially become apparent from the following description, or be understood through the practice of the present invention. Brief Description of the Drawings

[0018] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings in the following description are only some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings.

[0019] Figure 1 is the overall schematic diagram of the flow cytometry antibody premixing device according to the embodiment of the present invention; Figure 2 is the schematic diagram of the open switch door of the flow cytometry antibody premixing device according to the embodiment of the present invention; Figure 3 is the structural schematic diagram of the elastic movable member of the flow cytometry antibody premixing device according to the embodiment of the present invention; Figure 4 is the structural schematic diagram of the elastic movable member from another perspective of the flow cytometry antibody premixing device according to the embodiment of the present invention; Figure 5 is the structural schematic diagram of the ring body of the flow cytometry antibody premixing device according to the embodiment of the present invention; Figure 6It is the overall cross-sectional view before deformation of the flow cytometry antibody premixing device according to an embodiment of the present invention; Figure 7 It is the partially enlarged view before deformation of the flow cytometry antibody premixing device according to an embodiment of the present invention; Figure 8 It is the internal cross-sectional view after deformation of the flow cytometry antibody premixing device according to an embodiment of the present invention; Figure 9 It is the partially enlarged cross-sectional view after deformation of the flow cytometry antibody premixing device according to an embodiment of the present invention; Figure 10 It is the schematic diagram of the deformation of the annular body of the flow cytometry antibody premixing device before and after being pressed; Figure 11 It is the cross-sectional view of the lower end and the upper end plane of the limiting strip of the flow cytometry antibody premixing device according to an embodiment of the present invention; Figure 12 It is the comparison diagram of the overall details of the limiting strip and the gap of the flow cytometry antibody premixing device before and after deformation; Figure 13 It is the overall detailed view of the limiting strip and the clamping gap of the flow cytometry antibody premixing device according to an embodiment of the present invention.

[0020] Reference numerals: Box body 1, chamber 101, groove 102, visual glass 103, switch door 104; Elastic movable member 2, notch gap 201, movable flap 202; Pipette 3, nozzle ejector 31, disposable nozzle cone 32; Annular body 4; EP tube 5: Limiting strip 41, gap 410, sealing strip 42, clamping member 43, clamping gap 430. Detailed implementation manners

[0021] The embodiments of the present invention will be described in detail below. The embodiments described with reference to the drawings are exemplary. It should be understood that the specific embodiments described herein are only used to explain the present application and are not used to limit the present application.

[0022] It should be noted that when an element is referred to as being "fixed to" another element, it can be directly on the other element or there may also be an intermediate element. When an element is considered to be "connected" to another element, it can be directly connected to the other element or there may be an intermediate element at the same time.

[0023] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the technical field to which this invention belongs. The terms used in the specification of this invention are for the purpose of describing specific embodiments only and are not intended to limit the invention. The term "and / or" used herein includes any and all combinations of one or more of the related listed items.

[0024] In the specification and claims of this application and the accompanying drawings, the terms "first", "second", "third", etc. are used to distinguish different objects and are not used to describe a specific order. In addition, the terms "comprising" and "having" and any variations thereof are intended to cover non-exclusive inclusion. For example, a series of steps or units are included, or optionally, steps or units not listed are further included, or optionally, other steps or units inherent in these processes, methods, products or devices are further included.

[0025] Only parts related to this application are shown in the drawings, not all of the content. Before discussing the exemplary embodiments in more detail, it should be mentioned that some exemplary embodiments are described as processes or methods depicted as flowcharts. Although the flowcharts describe the operations (or steps) as sequential processes, many of the operations can be performed in parallel, concurrently, or simultaneously. In addition, the order of the operations can be rearranged. The process can be terminated when its operations are completed, but there can also be additional steps not included in the drawings. The process can correspond to a method, function, procedure, subroutine, subprogram, etc.

[0026] The terms "component", "module", "system", "unit", etc. used in this specification are used to represent computer-related entities, hardware, firmware, combinations of hardware and software, software, or software in execution. For example, a unit can be, but is not limited to, a process running on a processor, a processor, an object, an executable file, an execution thread, a program, and / or distributed between two or more computers. In addition, these units can be executed from various computer-readable media storing various data structures. A unit can communicate, for example, through signals with other systems through local and / or remote processes according to signals having one or more data packets (such as data from a second unit interacting with a local system, a distributed system, and / or a network. For example, the Internet interacting with other systems through signals).

[0027] Embodiment 1 As Figures 1 to 13 shown, this embodiment provides a flow cytometry antibody premixing device, which includes: A vertically arranged box body 1, having a chamber 101, and through holes are opened on the surface of the box body 1; The elastic movable member 2 passes through the through hole and extends into the chamber 101. The elastic movable member 2 is connected to the surface of the box body 1. The diameter of the elastic movable member 2 away from the surface of the box body 1 gradually shrinks, for the pipette 3 to pass through and extend into the chamber 101. Two notch slits 201 are formed on the side wall of the elastic movable member 2 to form two movable flaps 202. When there is no external force extrusion, under the elastic action of the elastic movable member 2, the two movable flaps 202 tend to extend and fit towards the notch slits 201 so that the chamber 101 below the movable flaps 202 forms a sealed space. When subjected to external force extrusion, the two movable flaps 202 gradually unfold in a direction away from each other, and the lower ends of the two movable flaps 202 change from a close state to a separated state. When the disposable nozzle cone 32 of the pipette 3 completely enters the chamber 101, the disposable nozzle cone 32 is limited by the lower ends of the two movable flaps 202; The annular body 4 is installed on the inner wall of the elastic movable member 2 and is located above the movable flap 202. When subjected to external force extrusion, the annular body 4 deforms so that the chamber 101 below the annular body 4 forms a sealed space; The EP tube 5 is installed in the chamber 101 and is used for pre-mixing flow cytometry antibodies.

[0028] It should be noted that the elastic movable member 2 is integrally in a semi-closed cavity frustum structure, and its upper surface has an opening for communication with the outside. The diameter of the elastic movable member 2 away from the surface of the box body 1 gradually shrinks. Specifically, the area S1 surrounded by the upper edge of the elastic movable member 2 is larger than the overall area S2 formed by the lower end face of the elastic movable member 2.

[0029] It should be noted that in this embodiment, the pipette 3 is a general-purpose pipette, including a nozzle ejector 31 and a disposable nozzle cone 32, and the disposable nozzle cone 32 is installed at the bottom end of the nozzle ejector 31.

[0030] It should be noted that in this embodiment, the annular body 4 includes: A plurality of limiting strips 41 are distributed at intervals in a ring shape, and there is a gap 410 between adjacent limiting strips 41. In this embodiment, the gap 410 is a fan-shaped gap; The sealing belt 42. Each sealing belt 42 passes through the gap 410 and is respectively connected to adjacent two limiting strips 41 to form a ring-shaped closed loop. When subjected to external force extrusion, the annular body 4 deforms so that the chamber 101 below the annular body 4 forms a sealed space.

[0031] It should be noted that in this embodiment, the sealing belt 42 has an arc-shaped contour deviating from the limiting strip 41, such as Figure 12As shown in (a) therein, it is a schematic diagram before the limiting strip is deformed under pressure. When extruded by an external force, the closed belt 42 extends towards the direction of the limiting strip 41 to increase the area of the region surrounded by the annular body 4, providing a displacement space for the pipette 3. As Figure 12 As shown in (b) therein, it is a schematic diagram of the enlarged gap after the limiting strip is deformed under pressure.

[0032] It should be noted that in this embodiment, the closed belt 42 has a first side arc L1 and a second side arc L2, where the first side arc L1 is the inner side arc close to the surface of the limiting strip 41, and the second side arc L2 is the outer side arc deviating from the surface of the limiting strip 41. The arc length of the first side arc L1 is greater than the arc length of the second side arc L2.

[0033] As Figure 10 shown, where Figure 10 As shown in (a) therein, it is a schematic diagram before the annular body is deformed under pressure. When no deformation occurs, the area surrounded by the overall inner edge formed by the limiting strip 41 is P1, and the lower surface of the nozzle ejector 31 of the pipette 3 is attached to the overall inner surface formed by the limiting strip 41; as Figure 10 As shown in (b) therein, it is a schematic diagram of the deformation after the annular body is deformed under pressure. In the fully deformed state, the area surrounded by the overall inner edge formed by the limiting strip 41 under extrusion is P2, and the cross-sectional area of the upper end of the nozzle ejector is S3, satisfying S1 > S3 ≥ P2 > P1; the cavity of the elastic movable member surrounded by the movable flap 202 can accommodate the disposable nozzle cone 32 of the pipette 3.

[0034] It should be noted that the closed belt 42 is made of an elastic material, which is convenient for unfolding under pressure to close the gap 410 between the limiting strips 41.

[0035] It should be noted that in this embodiment, the annular body 4 further includes: A clamping member 43, one end of which is connected to the side wall of the limiting strip 41 deviating from the elastic movable member 2. Among them, the end of the clamping member 43 far from the limiting strip 41 approaches the inner wall of the elastic movable member 2, that is, the whole clamping member 43 is in a radially outward radiation shape. The clamping member 43 and the upper surface of the limiting strip 41 and the inner wall of the elastic movable member 2 enclose a clamping gap 430 for the closed belt 42 to pass through, improving the overall tightness of the device, and the clamping member 43 is used to increase the limiting effect of the clamping gap 430.

[0036] It should be noted that in this embodiment, the two movable flaps 202 are symmetrically distributed, which is convenient for uniformly expanding outward under pressure to ensure that the pipette 3 moves vertically downward, avoiding the pipette 3 touching the movable flap 202 due to asymmetric deformation.

[0037] It should be noted that in this embodiment, a groove 102 is provided inside the box body 1 for fixing the EP tube 5, so that the disposable nozzle cone 32 with adsorbed antibody can extend into the EP tube 5. The elastic movable member 2 is located directly above the groove 102, facilitating the premixing of cell antibodies by a pipette gun.

[0038] According to the flow cytometry antibody premixing device of the present invention, it can ensure that the EP tube containing the antibody mixture in the box body is in a closed state isolated from the outside world throughout the entire process before and after antibody configuration, reducing contact with the outside world and lowering the risk of antibody mixture contamination. Specifically manifested as follows: By providing at least two notches at the lower end of the middle part of the elastic movable member to form at least two movable flaps, when the pipette gun enters the elastic movable member, a supporting force is generated between the surface of the pipette gun nozzle ejector and the inner wall of the limiting strip, and the movable flaps are pushed outward, facilitating the nozzle ejector and the disposable nozzle cone to enter the chamber of the box body. And due to the limiting effect of the clamping member on the sealing strip, the sealing strip maintains a tight closed fan-shaped gap state during the process of passing through the fan-shaped gap between the limiting strips to isolate contact with the outside world; During the process of the lower end of the movable flap opening, the lower end of the cavity of the elastic movable member surrounded by the movable flap communicates with the chamber of the box body, enabling the overall formed by at least two limiting strips to be a complete ring under the penetration of the sealing strip, facilitating separation from the upper end of the cavity of the elastic movable member, ensuring the sealing performance of the entire pipette gun during the process of entering the device when antibody mixing is carried out, and reducing the risk of antibody mixture contamination caused by the continuous opening of the EP tube.

[0039] Embodiment 2 On the basis of Embodiment 1, this embodiment makes further improvements and provides a flow cytometry antibody premixing device, wherein, At least one side surface of the box body 1 is provided with a visible glass 103, facilitating the observation of the operation process.

[0040] One side of the box body 1 is provided with a switch door 104, facilitating the placement of the EP tube 5 into the box body 1. A sealing ring is provided at the connection between the switch door 104 and the box body 1 for enhancing the tightness of the chamber 101 and reducing the risk of antibody mixture contamination.

[0041] This embodiment provides a flow cytometry antibody premixing method, including the following steps: S1, Premixing preparation: Open the EP tube cap, hold the EP tube body with forceps, place it in the groove inside the box body through the switch door, and then close the switch door; S2, Absorbing antibody: Install the disposable nozzle cone at the lower end of the nozzle ejector of the pipette gun, adjust the liquid absorption capacity of the pipette gun, and absorb the flow cytometry antibody; S3, Sealing and Pipetting: Vertically move the entire pipette gun downward from the upper end of the elastic movable part. When the disposable nozzle cone moves to the lower end of the cavity of the elastic movable part, the surface of the nozzle ejector adheres to the inner wall of the limit strip. As shown in (a) of Figure 11 , which is the cross-sectional view of the lower end plane of the limit strip. Since the pipette gun moves downward, the diameter of the nozzle ejector of the pipette gun in contact with the inner wall of the limit strip gradually increases. The limit strip is squeezed, resulting in an increase in the fan-shaped ring gap, thereby increasing the area enclosed by the limit strip. Among them, as shown in (b) of Figure 11 , which is the cross-sectional view of the upper end plane of the limit strip. The chamber is isolated from the external environment by squeezing the limit strip, forming a sealed environment inside the chamber. Due to the limiting effect of the clamping gap, during the increase of the fan-shaped ring gap, one end of the sealing band changes from vertically penetrating the fan-shaped ring gap to obliquely penetrating the fan-shaped ring gap. The whole sealing band is in a straightened state under force to seal the fan-shaped ring gap. Since the area enclosed by the two limit strips increases, it drives the movable flaps to gradually unfold outward. The lower ends of the two movable flaps change from a close state to a separated state. When the movable flaps are fully unfolded, the upper end of the nozzle ejector is squeezed and limited by the limit strip. The lower and middle parts of the disposable nozzle cone and the nozzle ejector enter the sealed chamber, and the antibody in the disposable nozzle cone is pushed along the inner wall of the EP tube into the EP tube; S4, Withdrawal of the Pipette Gun after Pipetting: Vertically move the entire pipette gun upward until the nozzle ejector is no longer squeezed and limited by the lower ends of the two movable flaps. Since the middle part of the nozzle ejector squeezes the limit strip and the lower ends of the movable flaps are in a separated state, it is beneficial for the disposable nozzle cone to move from the chamber to the lower end of the cavity of the elastic movable part. When the nozzle ejector gradually retracts, without external force extrusion, under the elastic action of the elastic movable part, the sealing band and the movable flaps reset. The two movable flaps tend to extend and fit towards the notch gap to form a sealed space in the chamber below the movable flaps. Finally, the pipette gun is removed from the elastic movable part to complete one pipetting; S5, Repeated Pipetting: Repeat steps S2 - S4 to sequentially add the antibodies to be mixed into the EP tube. Until after the last antibody is pushed into the EP tube, repeatedly aspirate the pipette gun to mix the antibodies until they are completely mixed; S6, Collection of the Mixed Sample: Open the switch door, use tweezers to loosely cover the tube cap of the EP tube on the mouth of the EP tube to prevent spillage when taking it out. Clamp the upper part of the tube body of the EP tube with the tweezers, take out the EP tube containing the antibody mixture from the groove in the box, and cover the tube cap tightly for standby.

[0042] In the description of the present invention, it should be understood that the orientation or positional relationships indicated by the terms "center", "longitudinal", "transverse", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential", etc. are based on the orientation or positional relationships shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of the invention.

[0043] In the description of this specification, the description with reference to terms such as "one embodiment", "some embodiments", "schematic embodiments", "examples", "specific examples", or "some examples", etc. means that the specific features, structures, materials, or characteristics described in connection with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic expressions of the above terms do not necessarily refer to the same embodiment or example.

[0044] Obviously, the described embodiments are only a part of the embodiments of the present application, rather than all of the embodiments. The mention of "embodiment" in this article means that the specific features, structures, or characteristics described in connection with the embodiment can be included in at least one embodiment of this application. The appearance of this phrase at various positions in the specification does not necessarily refer to the same embodiment, nor is it an independent or alternative embodiment mutually exclusive with other embodiments. Those skilled in the art can explicitly and implicitly understand that the embodiments described herein can be combined with other embodiments. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative efforts belong to the scope protected by the present application.

[0045] Although the embodiments of the present invention have been shown and described, those of ordinary skill in the art can understand that various changes, modifications, substitutions, and variations can be made to these embodiments without departing from the principles and purposes of the present invention, and the scope of the present invention is defined by the claims and their equivalents.

Claims

1. A flow cytometry antibody premixing device, characterized in that Comprising: A box body (1) having a chamber (101), and through holes are formed on the surface of the box body (1); An elastic movable member (2) passing through the through hole and extending into the chamber (101), the elastic movable member (2) being connected to the box body (1), wherein the diameter of the elastic movable member (2) far from the box body (1) gradually contracts, for a pipette gun (3) to pass through and extend into the chamber (101), and two notch slits (201) are formed on the side wall of the elastic movable member (2) to form two movable flaps (202). When there is no external force extrusion, the two movable flaps (202) tend to extend and fit towards the notch slits (201) so that a sealed space is formed in the chamber (101) below the movable flaps (202); A ring body (4) installed on the inner wall of the elastic movable member (2) and located above the movable flaps (202). When externally extruded, the ring body (4) deforms so that a sealed space is formed in the chamber (101) below the ring body (4); An EP tube (5) installed in the chamber (101) for pre-mixing flow cytometry antibodies.

2. The flow cytometry antibody premixing device according to claim 1, wherein The ring body (4) includes: A plurality of limiting strips (41) distributed at intervals in a ring shape, and a gap (410) is provided between adjacent limiting strips (41); A sealing belt (42), and each sealing belt (42) passes through the gap (410) and is respectively connected to adjacent two limiting strips (41) to form a ring-shaped closed loop.

3. The flow cytometry antibody premixing device according to claim 2, wherein The sealing belt (42) has an arc-shaped profile deviating from the limiting strip (41).

4. The flow cytometry antibody premixing device according to claim 2, wherein The ring body (4) further includes: A clamping member (43) with one end connected to the side wall of the limiting strip (41) deviating from the elastic movable member (2).

5. The flow cytometry antibody premixing device according to claim 3, wherein The sealing belt (42) has a first side arc and a second side arc, wherein the first side arc is the inner side arc close to the surface of the limiting strip (41), and the second side arc is the outer side arc deviating from the surface of the limiting strip (41), and the arc length of the first side arc is greater than the arc length of the second side arc.

6. The flow cytometry antibody premixing device according to claim 4, wherein The end of the clamping member (43) far from the limiting strip (41) approaches the inner wall of the elastic movable member (2).

7. The flow cytometry antibody premixing device according to claim 5, wherein The sealing belt (42) is made of an elastic material.

8. The flow cytometry antibody premixing device according to claim 1, wherein, The two movable flaps (202) are symmetrically distributed.

9. The flow cytometry antibody premixing device according to claim 1, wherein A groove (102) is provided on the inner side of the box body (1).

10. The flow cytometry antibody premixing device according to claim 9, characterized in that, A visual glass (103) is provided on any side surface of the box body (1).

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