Microbial agent for sewage treatment as well as preparation method and application of microbial agent

Abstract

The invention belongs to the technical field of sewage treatment, and relates to a microbial agent for sewage treatment as well as a preparation method and application thereof. The microbial agent for sewage treatment is prepared from the following components in parts by weight: 10-20 parts of bentonite, 6-10 parts of humic acid, 15-25 parts of a compound microbial agent and 10-20 parts of charcoal. The bentonite, humic acid, biochar and the compound microbial agent complement and cooperate with one another, a multi-level virtuous cycle is formed in the aspect of removing organic pollution such as phenol or heavy metal pollution, and the sewage treatment effect is improved.

Description

Technical Field

[0001] The present invention belongs to the technical field of sewage treatment, and relates to a microbial agent for sewage treatment, a preparation method thereof and an application thereof. Background Art

[0002] More than 180 kinds of organic pollutants have been found in groundwater, including aromatic hydrocarbons, halogenated hydrocarbons, pesticides, etc., and the quantity and variety are still increasing rapidly. Even some pesticides not registered for use have been found. The research on groundwater pollution has shifted from inorganic pollution to organic pollution, and trace organic pollution has become the primary problem in the field of groundwater environmental protection.

[0003] At present, the water treatment methods are divided into physical method, chemical method and biological method. Compared with the first two methods, due to advantages such as low cost, high treatment efficiency and no easy generation of secondary pollution, the biological method has been widely applied to the treatment of various sewage. The biological method for treating sewage is to add microorganisms to the sewage and use the microorganisms to adsorb, decompose and oxidize pollutants, so as to convert the pollutants into stable and harmless substances, achieving the purpose of reducing water pollution. However, the traditional biological treatment method may have the problem of incomplete treatment effect when treating sewage with more complex components. Summary of the Invention

[0004] In view of this, the purpose of the present invention is to provide a microbial agent for sewage treatment, a preparation method thereof and an application thereof. The present invention utilizes the complementary and synergistic effects of bentonite, humic acid, biochar and the composite microbial agent to form a multi-level virtuous cycle in removing organic pollution such as phenol or heavy metal pollution, improving the treatment effect on sewage.

[0005] To achieve the above purpose, the present application adopts the following technical solutions:

[0006] In the first aspect, the present invention provides a microbial agent for sewage treatment, and the microbial agent for sewage treatment is made of the following components by weight: 10-20 parts of bentonite, 6-10 parts of humic acid, 15-25 parts of composite microbial agent and 10-20 parts of biochar.

[0007] Further, the composite microbial agent is Bacillus licheniformis, Pseudomonas putida and Bacillus thuringiensis with a mass ratio of 1:2-4:3-5.

[0008] Further, the microbial agent for sewage treatment is made of the following components by weight: 15 parts of bentonite, 8 parts of humic acid, 20 parts of composite microbial agent and 15 parts of biochar.

[0009] Furthermore, the microbial agent for sewage treatment is made of the following components in parts by weight: 20 parts of bentonite, 6 parts of humic acid, 25 parts of composite microbial agent and 10 parts of biochar.

[0010] Furthermore, the microbial agent for sewage treatment is made of the following components in parts by weight: 10 parts of bentonite, 10 parts of humic acid, 15 parts of composite microbial agent and 20 parts of biochar.

[0011] Furthermore, the composite microbial agent is Bacillus licheniformis, Pseudomonas putida and Bacillus thuringiensis in a mass ratio of 1:3:4.

[0012] In a second aspect, the present invention provides a method for preparing the above-mentioned microbial agent for sewage treatment, wherein the preparation method is as follows: (1) preparing biochar: crushing wheat straw and putting it into a biochar high-temperature cracking furnace, pyrolyzing it at 450°C-550°C for 1.5-2.5 hours under nitrogen atmosphere protection to generate biochar, crushing it, and passing it through a 200-300 mesh sieve to obtain wheat straw biochar; (2) uniformly mixing Bacillus licheniformis, Pseudomonas putida and Bacillus thuringiensis according to the mass ratio to obtain a composite microbial agent; (3) weighing bentonite, humic acid and biochar according to weight parts, mixing them to obtain a mixture one; (4) mixing the composite microbial agent and the mixture one to obtain a microbial agent for sewage treatment.

[0013] Furthermore, the pyrolysis temperature in step (1) is 500°C.

[0014] Furthermore, the pyrolysis time in step (1) is 2 hours.

[0015] In a third aspect, the present invention provides the use of the microbial agent for sewage treatment obtained by the above preparation method in repairing a water environment polluted by phenolic organic compounds.

[0016] Furthermore, the phenolic organic pollution is phenol.

[0017] The present invention provides the use of the microbial agent for sewage treatment obtained by the above preparation method in repairing a water environment polluted by heavy metals.

[0018] Bentonite has a large specific surface area, cation exchange capacity, and good adsorption properties. However, due to the extremely hydrophilic nature of the silica structure on the bentonite surface and the hydrolysis of interlayer cations, a thin water film usually forms on the bentonite surface, making it ineffective in adsorbing hydrophobic organic pollutants.

[0019] Biochar is a carbon-rich solid material produced by pyrolyzing biomass waste, sludge, etc. at a certain high temperature under anaerobic or anoxic conditions. It has the characteristics of loose structure and easy regulation of specific surface area. At the same time, its surface is rich in functional groups with strong adsorption ability, such as hydroxyl, carboxyl, and amino groups.

[0020] Humic acid substances are oligomers or polymers containing various polar functional groups formed by the decomposition or recombination of animals and plants under chemical or microbial action. Humic acid contains many phenolic hydroxyl groups and has a very strong reducing effect. Due to the existence of many special structures in humic acid, it has a strong binding ability to some aromatic organic compounds, such as pyrene, fluorinated anthracene, phenanthrene, nitrophenol, etc.

[0021] Composite microbial communities are formed by combining two or more functional strains. The ecological interactions between strains are mainly divided into six forms: mutualism, commensalism, amensalism, predation, competition, and neutralism. Designing and constructing appropriate strain interaction relationships is of great significance for maintaining the structural stability of composite microbial communities.

[0022] Compared with the prior art, the beneficial effects achieved by the present invention are as follows:

[0023] The strains in the present invention are mutually symbiotic, which can promote the growth of strains in polluted environments. At the same time, the strains can share or exchange nutrients, thereby improving the degradation effect of pollutants.

[0024] The humic acid of the present invention can optimize the generation and reproduction environment of microorganisms, promote the degradation of wastewater, and accelerate the decomposition and removal of organic matter by degrading organic matter together with microorganisms.

[0025] The present invention utilizes the complementary and synergistic effects of bentonite, humic acid, biochar, and composite microbial agents to form a multi-level virtuous cycle in the removal of organic pollution such as phenol and heavy metal pollution, improving the treatment effect of sewage. Brief Description of the Drawings

[0026] In order to more clearly illustrate the technical solutions in the present invention or the prior art, the drawings required for the specific embodiments will be briefly introduced below.

[0027] Figure 1 The removal rate of phenol at 30 min for each treatment.

[0028] Figure 2 The removal rate of COD at 30 min for each treatment.

[0029] Figure 3 The removal rate of phenol at 50 min for each treatment.

[0030] Figure 4Removal rate of COD when each treatment lasts for 50 min.

[0031] Figure 5 Removal rate of each treatment for Pb 2+

[0032] Figure 6 Removal rate of each treatment for Zn 2+ Detailed implementation manners

[0033] The various exemplary implementation manners of the present invention will now be described in detail. This detailed description should not be considered as a limitation of the present invention, but rather as a more detailed description of certain aspects, characteristics, and implementation schemes of the present invention.

[0034] It should be understood that the terms described in the present invention are only for describing particular implementation manners and are not used to limit the present invention. Additionally, for the numerical ranges in the present invention, it should be understood that each intermediate value between the upper and lower limits of the range is also specifically disclosed. Each intermediate value within any stated value or stated range, as well as each smaller range between any other stated value or intermediate value within the stated range, is also included in the present invention. The upper and lower limits of these smaller ranges may be independently included or excluded from the range.

[0035] Unless otherwise specified, all technical and scientific terms used herein have the same meaning as commonly understood by those of ordinary skill in the art to which the present invention pertains. Although the present invention only describes preferred methods and materials, any methods and materials similar or equivalent to those described herein can also be used in the implementation or testing of the present invention. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and / or materials related to the documents. In case of conflict with any incorporated document, the content of this specification shall prevail.

[0036] The reagents and raw materials used in the following examples and effect verification can be obtained from commercial channels unless otherwise specified.

[0037] Example 1

[0038] A microbial inoculant for sewage treatment is prepared from the following components by weight: 15 parts of bentonite, 8 parts of humic acid, 20 parts of composite microbial inoculant, and 15 parts of biochar.

[0039] The composite microbial inoculant is Bacillus licheniformis, Pseudomonas putida, and Bacillus thuringiensis with a mass ratio of 1:3:4.

[0040] ​​The preparation method is as follows: (1) Preparation of biochar: Wheat straw is crushed and then put into a high-temperature pyrolysis furnace for biochar. Under the protection of a nitrogen atmosphere, it is pyrolyzed at 500 °C for 2 h to generate biochar, which is crushed and sieved through a 200-mesh sieve to obtain wheat straw biochar; (2) Bacillus licheniformis, Pseudomonas putida, and Bacillus thuringiensis are mixed evenly according to the said mass ratio to obtain a composite microbial agent; (3) Weigh bentonite, humic acid, and biochar by weight parts, and mix them to obtain mixture one; (4) Mix the composite microbial agent and mixture one to obtain the microbial agent for sewage treatment.

[0041] Example 2

[0042] A microbial agent for sewage treatment is made of the following components by weight parts: 20 parts of bentonite, 6 parts of humic acid, 25 parts of composite microbial agent, and 10 parts of biochar.

[0043] The said composite microbial agent is Bacillus licheniformis, Pseudomonas putida, and Bacillus thuringiensis with a mass ratio of 1:2:5.

[0044] The preparation method is as follows: (1) Preparation of biochar: Wheat straw is crushed and then put into a high-temperature pyrolysis furnace for biochar. Under the protection of a nitrogen atmosphere, it is pyrolyzed at 450 °C for 2.5 h to generate biochar, which is crushed and sieved through a 300-mesh sieve to obtain wheat straw biochar; (2) Bacillus licheniformis, Pseudomonas putida, and Bacillus thuringiensis are mixed evenly according to the said mass ratio to obtain a composite microbial agent; (3) Weigh bentonite, humic acid, and biochar by weight parts, and mix them to obtain mixture one; (4) Mix the composite microbial agent and mixture one to obtain the microbial agent for sewage treatment.

[0045] Example 3

[0046] A microbial agent for sewage treatment is made of the following components by weight parts: 10 parts of bentonite, 10 parts of humic acid, 15 parts of composite microbial agent, and 20 parts of biochar.

[0047] The said composite microbial agent is Bacillus licheniformis, Pseudomonas putida, and Bacillus thuringiensis with a mass ratio of 1:4:3.

[0048] The preparation method is as follows: (1) Preparation of biochar: Wheat straw is crushed and then put into a high-temperature pyrolysis furnace for biochar. Under the protection of a nitrogen atmosphere, it is pyrolyzed at 550 °C for 1.5 h to generate biochar, which is crushed and sieved through a 200-mesh sieve to obtain wheat straw biochar; (2) Bacillus licheniformis, Pseudomonas putida, and Bacillus thuringiensis are mixed evenly according to the said mass ratio to obtain a composite microbial agent; (3) Weigh bentonite, humic acid, and biochar by weight parts, and mix them to obtain mixture one; (4) Mix the composite microbial agent and mixture one to obtain the microbial agent for sewage treatment.

[0049] Example 4

[0050] A microbial agent for sewage treatment is prepared from the following components by weight: 15 parts of bentonite, 10 parts of humic acid, 15 parts of composite microbial agents and 15 parts of biochar.

[0051] The composite microbial agent comprises Bacillus licheniformis, Pseudomonas putida and Bacillus thuringiensis in a mass ratio of 1:2:3.

[0052] The preparation method is as follows: (1) preparation of biochar: wheat straw is crushed and put into a biochar high-temperature cracking furnace, pyrolyzed at 500°C for 2 hours under nitrogen atmosphere to generate biochar, crushed, and passed through a 300-mesh sieve to obtain wheat straw biochar; (2) Bacillus licheniformis, Pseudomonas putida and Bacillus thuringiensis are mixed uniformly according to the mass ratio to obtain a composite microbial agent; (3) bentonite, humic acid and biochar are weighed according to weight, mixed to obtain a mixture one; (4) the composite microbial agent and the mixture one are mixed to obtain a microbial agent for sewage treatment.

[0053] Example 5

[0054] A microbial agent for sewage treatment is prepared from the following components by weight: 10 parts of bentonite, 10 parts of humic acid, 20 parts of composite microbial agents and 20 parts of biochar.

[0055] The composite microbial agent comprises Bacillus licheniformis, Pseudomonas putida and Bacillus thuringiensis in a mass ratio of 1:4:5.

[0056] The preparation method is as follows: (1) preparation of biochar: wheat straw is crushed and put into a biochar high-temperature cracking furnace, and pyrolyzed at 450°C for 2.5 hours under nitrogen atmosphere to generate biochar, which is crushed and passed through a 200-mesh sieve to obtain wheat straw biochar; (2) Bacillus licheniformis, Pseudomonas putida and Bacillus thuringiensis are mixed uniformly according to the mass ratio to obtain a composite microbial agent; (3) bentonite, humic acid and biochar are weighed according to weight, mixed to obtain a mixture one; (4) the composite microbial agent and the mixture one are mixed to obtain a microbial agent for sewage treatment.

[0057] Example 6

[0058] A microbial agent for sewage treatment is prepared from the following components by weight: 20 parts of bentonite, 8 parts of humic acid, 25 parts of composite microbial agents and 10 parts of biochar.

[0059] The composite microbial agent comprises Bacillus licheniformis, Pseudomonas putida and Bacillus thuringiensis in a mass ratio of 1:4:5.

[0060] The preparation method is as follows: (1) Preparation of biochar: Wheat straw is crushed and put into a high-temperature pyrolysis furnace for biochar. Under the protection of a nitrogen atmosphere, it is pyrolyzed at 550 °C for 1.5 h to generate biochar, which is then crushed and sieved through a 300-mesh sieve to obtain wheat straw biochar; (2) Bacillus licheniformis, Pseudomonas putida, and Bacillus thuringiensis are mixed evenly according to the said mass ratio to obtain a composite microbial agent; (3) Weigh bentonite, humic acid, and biochar by weight parts, mix them to obtain a mixture one; (4) Mix the composite microbial agent and mixture one to obtain the microbial agent for sewage treatment.

[0061] Comparative Example 1

[0062] 13 parts of humic acid, 25 parts of composite microbial agent, and 20 parts of biochar.

[0063] Compared with Example 1, the difference is that it does not contain bentonite, and the dosages of humic acid, composite microbial agent, and biochar are different.

[0064] The preparation method refers to Example 1.

[0065] Comparative Example 2

[0066] 18 parts of bentonite, 22 parts of composite microbial agent, and 18 parts of biochar. <8000152>Compared with Example 1, the difference is that it does not contain humic acid, and the dosages of bentonite, composite microbial agent, and biochar are different.

[0068] The preparation method refers to Example 1.

[0069] Comparative Example 3

[0070] 20 parts of bentonite, 18 parts of humic acid, and 20 parts of biochar.

[0071] Compared with Example 1, the difference is that it does not contain the composite microbial agent, and the dosages of bentonite, humic acid, and biochar are different.

[0072] The preparation method refers to Example 1.

[0073] Comparative Example 4

[0074] 20 parts of bentonite, 13 parts of humic acid, and 25 parts of composite microbial agent.

[0075] Compared with Example 1, the difference is that it does not contain biochar, and the dosages of bentonite, humic acid, and composite microbial agent are different.

[0076] The preparation method refers to Example 1.

[0077] Comparative Example 5

[0078] 15 parts of bentonite, 8 parts of humic acid, 20 parts of compound microbial inoculum, and 15 parts of biochar.

[0079] Compared with Example 1, the difference lies in the different compound microbial inoculum. The compound microbial inoculum is Bacillus licheniformis and Bacillus thuringiensis with a mass ratio of 2:5.

[0080] The preparation method refers to Example 1.

[0081] Comparative Example 6

[0082] 15 parts of bentonite, 8 parts of humic acid, 20 parts of compound microbial inoculum, and 15 parts of biochar.

[0083] Compared with Example 1, the difference lies in the different compound microbial inoculum. The compound microbial inoculum is Bacillus licheniformis, Bacillus amyloliquefaciens, Bacillus cereus, and Bacillus thuringiensis with a mass ratio of 1:3:2:2.

[0084] The preparation method refers to Example 1.

[0085] Test Example 1 Experimental study on the adsorption of phenol in sewage by the microbial inoculum for sewage treatment of the present invention

[0086] Dissolve 1.0 g of phenol in deionized water and make up the volume to 1000 mL to obtain a phenol standard stock solution with a concentration of 1.0 g / L. Dilute it to the required concentration with deionized water during the experiment.

[0087] In a 1000 mL conical flask, add 800 mL of simulated phenol-polluted aqueous solution with a concentration of 50 mg / L and 35 g of the microbial inoculum for sewage treatment. A total of 9 treatments are set up in the test, namely Example 1 group (Group A), Example 2 group (Group B), Example 3 group (Group C), Comparative Example 1 group (Group D), Comparative Example 2 group (Group E), Comparative Example 3 group (Group F), Comparative Example 4 group (Group G), Comparative Example 5 group (Group H), and Comparative Example 6 group (Group I). Each treatment is set with 3 replicates.

[0088] Each treatment group is shaken at a speed of 120 r / min at room temperature for 50 min, then centrifuged at high speed for 5 min, the supernatant is taken, and the residual amount of phenol is measured. Calculate the removal rate of phenol in water: q = [(co - cs) / co] × 100%.

[0089] Among them, co is the phenol concentration before adsorption; cs is the phenol concentration after adsorption.

[0090] The phenol concentration in the aqueous solution is determined by 4-aminoantipyrine spectrophotometry.

[0091] Experimental results

[0092] As Figure 1 、 2As shown, for each treatment group, at 30 minutes of treatment, the removal rates of phenol by Group A, Group B, and Group C were 73.5%, 69.3%, and 65.8% respectively, which were better than those of Group D, Group E, Group F, Group G, Group H, and Group I. The removal rates of COD by Group A, Group B, and Group C were 63.2%, 59.3%, and 55.1% respectively, which were better than those of Group D, Group E, Group F, Group G, Group H, and Group I.

[0093] As shown in Table 1, Figure 3 , 4 As shown, for each treatment group, at 60 minutes of treatment, the removal rates of phenol by Group A, Group B, and Group C were 97.1%, 92.0%, and 91.2% respectively, which were better than those of Group D, Group E, Group F, Group G, Group H, and Group I. The removal rates of COD by Group A, Group B, and Group C were 89.7%, 86.7%, and 87.1% respectively, which were better than those of Group D, Group E, Group F, Group G, Group H, and Group I.

[0094] Table 1 Removal rates of phenol and COD at 60 minutes under each treatment

[0095]

[0096] The present invention utilizes bentonite, humic acid, biochar and composite microbial agents to complement and cooperate with each other, forming a multi-level virtuous cycle in the removal of organic pollutants such as phenol, and improving the treatment effect of sewage.

[0097] Test Example 2 Experimental study on the adsorption of heavy metals in sewage by the microbial agent for sewage treatment of the present invention

[0098] 0.5 g of Pb(NO3)2 and 0.5 g of ZnSO4·7H2O were added to deionized water and made up to 1000 mL to obtain a heavy metal standard stock solution with a concentration of 1.0 g / L. During the experiment, it was diluted to the required concentration with deionized water.

[0099] In a 1000 mL conical flask, 800 mL of a simulated heavy metal polluted aqueous solution with a concentration of 80 mg / L and 45 g of the microbial agent for sewage treatment were added. A total of 9 treatments were set up in the test, namely Example 1 group (Group A), Example 2 group (Group B), Example 3 group (Group C), Comparative Example 1 group (Group D), Comparative Example 2 group (Group E), Comparative Example 3 group (Group F), Comparative Example 4 group (Group G), Comparative Example 5 group (Group H), and Comparative Example 6 group (Group I). Each treatment was set with 3 replicates.

[0100] Each treatment group was shaken at a speed of 140 r / min at room temperature for 60 minutes, then centrifuged at high speed for 8 minutes, and the supernatant was taken to measure the residual amount of heavy metals and calculate the removal rate of heavy metals in water: q = [(co - cs) / co] × 100%.

[0101] Wherein, co is the heavy metal concentration before adsorption; cs is the heavy metal concentration after adsorption.

[0102] Pb in the solution 2+ and Zn 2+ concentrations were determined by atomic absorption spectrophotometer.

[0103] Experimental results

[0104] As shown in Table 2 Figure 5 and 6 shown, for each treatment group, at 60 min of treatment, the removal rates of Pb 2+ by Group A, Group B, and Group C were 92.7%, 89.8%, and 90.2% respectively, which were superior to those of Group D, Group E, Group F, Group G, Group H, and Group I. The removal rates of Zn 2+ by Group A, Group B, and Group C were 83.4%, 79.5%, and 80.5% respectively, which were superior to those of Group D, Group E, Group F, Group G, Group H, and Group I.

[0105] Table 2 Removal rates of Pb 2+ and Zn 2+ at 60 min under each treatment

[0106]

[0107] The present invention utilizes bentonite, humic acid, biochar, and composite microbial inoculants to complement and synergize with each other, and has a significant effect on treating heavy metal - contaminated sewage.

[0108] The technical features of the above - described embodiments can be combined arbitrarily. For the sake of brevity of description, not all possible combinations of the technical features in the above - described embodiments are described. However, as long as there is no contradiction in the combination of these technical features, it should be considered as the scope recorded in this specification.

[0109] The above are only the embodiments of the present invention. Specific technical solutions and / or common knowledge such as characteristics well - known in the art are not described in detail herein. It should be noted that for those skilled in the art, without departing from the technical solution of the present invention, several deformations and improvements can be made, and these should also be regarded as the protection scope of the present invention, which will not affect the implementation effect of the present invention and the practicality of the patent. The protection scope required by this application should be based on the content of its claims, and the specific implementation manners described in the specification can be used to interpret the content of the claims.

Claims

1. A microbial inoculant for sewage treatment, characterized in that, The microbial agent for sewage treatment is prepared from the following components by weight: 10-20 parts of bentonite, 6-10 parts of humic acid, 15-25 parts of a composite microbial agent and 10-20 parts of biochar; the composite microbial agent comprises Bacillus licheniformis, Pseudomonas putida and Bacillus thuringiensis in a mass ratio of 1:2-4:3-5.

2. The microbial inoculant for sewage treatment according to claim 1, characterized in that The microbial agent for sewage treatment is prepared from the following components by weight: 15 parts of bentonite, 8 parts of humic acid, 20 parts of composite microbial agent and 15 parts of biochar.

3. The microbial inoculant for sewage treatment according to claim 1, characterized in that, The microbial agent for sewage treatment is prepared from the following components by weight: 20 parts of bentonite, 6 parts of humic acid, 25 parts of composite microbial agent and 10 parts of biochar.

4. The microbial inoculant for sewage treatment according to claim 1, characterized in that, The microbial agent for sewage treatment is prepared from the following components by weight: 10 parts of bentonite, 10 parts of humic acid, 15 parts of composite microbial agent and 20 parts of biochar.

5. The microbial inoculant for sewage treatment according to claim 1, wherein The composite microbial agent comprises Bacillus licheniformis, Pseudomonas putida and Bacillus thuringiensis in a mass ratio of 1:2-4:3-5.

6. The microbial inoculant for sewage treatment according to claim 5, wherein The composite microbial agent comprises Bacillus licheniformis, Pseudomonas putida and Bacillus thuringiensis in a mass ratio of 1:3:

4.

7. The microbial inoculant for sewage treatment according to claim 1, wherein, The preparation method comprises the following steps: (1) preparing biochar: crushing wheat straw and putting it into a biochar high-temperature cracking furnace, pyrolyzing it at 450° C.-550° C. for 1.5-2.5 hours under nitrogen atmosphere protection to generate biochar, crushing it, and passing it through a 200-300 mesh sieve to obtain wheat straw biochar; (2) uniformly mixing Bacillus licheniformis, Pseudomonas putida and Bacillus thuringiensis according to the mass ratio to obtain a composite microbial agent; (3) weighing bentonite, humic acid and biochar according to parts by weight, mixing them to obtain a mixture 1; and (4) mixing the composite microbial agent and the mixture 1 to obtain a microbial agent for sewage treatment.

8. The microbial inoculum for sewage treatment according to claim 7, characterized in that, The pyrolysis temperature in step (1) is 500°C.

9. The microbial inoculant for sewage treatment according to claim 7, wherein The pyrolysis time in step (1) is 2 hours.

10. Use of the microbial agent for sewage treatment according to claim 1 in repairing a water environment polluted by phenolic organic compounds or heavy metals.

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