Organic pigment and extraction and application thereof

Abstract

The invention relates to the technical field of substance extraction, in particular to an organic pigment and extraction and application thereof. The preparation method comprises the following steps: firstly, pretreating pitaya peel, drying, and crushing to obtain pitaya peel powder; and uniformly mixing the pitaya peel powder with water, performing ultrasonic extraction, and performing post-treatment to obtain the organic pigment. A water extraction method is adopted for extraction, ethanol residues in a traditional alcohol extraction method are avoided, and therefore the method is simpler, safer and more environmentally friendly, and the obtained pigment is safer; the organic pigment provided by the invention has excellent anti-oxidation and anti-inflammatory properties, and also has good ultraviolet resistance and blue light resistance.

Description

Technical Field

[0001] The present invention relates to the technical field of substance extraction, and in particular to an organic pigment and its extraction and application. Background Art

[0002] The organic pigment in pitaya peel is betacyanin. Betacyanin is a natural red pigment and one of the commonly known natural pigments. Betacyanin exists in many plants. Red-fleshed pitaya also contains betacyanin, especially in relatively high content in the peel. Betacyanin can mainly achieve a super antioxidant effect, and can also reduce cell oxidation, and can play a good role in alleviating chronic inflammation. In addition, it can achieve a synergistic anti-cancer effect.

[0003] Existing methods for extracting organic pigments from pitaya peel include water extraction method, alcohol extraction method, enzymatic hydrolysis method, and microwave-assisted extraction method. The alcohol extraction method has a risk of solvent residue, and organic solvents may remain, requiring additional purification steps; the temperature control of microwave-assisted extraction is strict, overheating may cause pigment decomposition, and microwave equipment is expensive and complex to maintain; the enzymatic hydrolysis method has a high cost, the enzyme preparation is expensive, the process is complex, and the enzyme type, concentration and reaction time need to be optimized. In addition, how to further explore the properties of organic pigments in pitaya remains the pursuit of those skilled in the art.

[0004] Therefore, it is still crucial to provide a technical solution that can solve the above problems. Summary of the Invention

[0005] In order to solve the above problems, the purpose of the present invention is to provide an organic pigment and its extraction and application. The present invention uses the water extraction method to avoid ethanol residue in the traditional alcohol extraction method, so as to be safer and more environmentally friendly. In addition to exploring its antioxidant and anti-inflammatory properties, the anti-ultraviolet and anti-blue light properties of betacyanin are also explored.

[0006] The purpose of the present invention can be achieved by the following technical solutions:

[0007] The first purpose of the present invention is to provide a method for extracting an organic pigment, comprising the following steps:

[0008] (S1) Pretreat the pitaya peel and then perform drying treatment and pulverization treatment to obtain pitaya peel powder;

[0009] (S2) Mix the pitaya peel powder prepared in step (S1) with water and perform ultrasonic extraction, and then perform post-treatment to obtain the organic pigment.

[0010] In one embodiment of the present invention, in step (S1), the pretreatment is to wash the pitaya peel with clean water, then cut off the rhizomes and the old and withered parts on the peel, cut the peel into pieces and then perform a freezing treatment.

[0011] In one embodiment of the present invention, in step (S1), during the freezing treatment, the temperature is -20 to -15 °C and the time is 6 to 8 h;

[0012] The drying treatment is selected from one of hot air drying, spray drying, freeze drying or microwave vacuum drying;

[0013] Preferably, the drying treatment is freeze drying treatment. During the freeze drying treatment, the temperature is -50 to -40 °C and the time is 24 to 48 h;

[0014] The particle size of the pitaya peel powder is ≤80 mesh.

[0015] In one embodiment of the present invention, in step (S2), the dosage ratio of the pitaya peel powder to water is 1 g: 10 - 50 mL;

[0016] Preferably, the dosage ratio of the pitaya peel powder to water is 1 g: 40 mL.

[0017] In one embodiment of the present invention, in step (S2), during the ultrasonic extraction, the temperature is 15 - 55 °C, the power is 200 - 400 W, and the time is 15 - 55 min;

[0018] Preferably, during the ultrasonic extraction, the temperature is 45 °C, the power is 300 W, and the time is 35 min.

[0019] In one embodiment of the present invention, in step (S2), the post-treatment is, after the ultrasonic treatment ends, centrifuging the obtained ultrasonic extraction liquid, filtering the obtained upper liquid, and subjecting the obtained filtrate to heating and concentration (to avoid ethanol residue caused by ethanol rotary evaporation) and then freeze drying.

[0020] In one embodiment of the present invention, during the centrifuging process, the rotation speed is 7000 - 9000 r / min and the time is 5 - 15 min;

[0021] Preferably, during the centrifuging process, the rotation speed is 8000 r / min and the time is 10 min;

[0022] During the filtering process, the pore size of the filter membrane is 40 - 80 μm;

[0023] Preferably, during the filtering process, the pore size of the filter membrane is 40 μm;

[0024] During the heating and concentration process, the temperature is 40 - 60 °C and the time is 6 - 8 h;

[0025] Preferably, during the heating and concentration process, the temperature is 55°C, the time is 6 h, and the concentration multiple is 5 to 40 times (as long as it can be concentrated to 1 / 5 to 1 / 40 of the filtrate volume).

[0026] During the freeze-drying process, the temperature is -50 to -40°C, and the time is 24 to 48 h.

[0027] Preferably, during the freeze-drying process, the temperature is -50°C, and the time is 48 h.

[0028] The second object of the present invention is to provide an organic pigment obtained by the above method. This organic pigment has excellent antioxidant and anti-inflammatory properties, and also has ultraviolet resistance (280 - 400 nm) and blue light resistance (400 - 500 nm).

[0029] The third object of the present invention is to provide an application of the organic pigment in the preparation of cosmetics (such as blush products or lip cosmetics) and / or foods, or in the field of clothing dyeing.

[0030] Compared with the prior art, the present invention has the following beneficial effects:

[0031] The present invention uses the water extraction method, avoiding ethanol residues in the traditional alcohol extraction method, thus being simpler, safer, more environmentally friendly, and the obtained pigment is safer; the organic pigment provided by the present invention has excellent antioxidant and anti-inflammatory properties, and also has good ultraviolet resistance and blue light resistance. Description of the Drawings

[0032] Figure 1 is the standard curve of betacyanin;

[0033] Figure 2 is the influence diagram of ultrasonic temperature on the extraction content of betacyanin;

[0034] Figure 3 is the influence diagram of the solid-liquid ratio on the extraction content of betacyanin;

[0035] Figure 4 is the influence diagram of ultrasonic time on the extraction content of betacyanin;

[0036] Figure 5 is the influence diagram of ultrasonic power on the extraction content of betacyanin;

[0037] Figure 6 is the DPPH free radical scavenging ability diagram;

[0038] Figure 7 is the ABTS free radical scavenging ability diagram;

[0039] Figure 8 Graph of the scavenging ability of hydroxyl radicals;

[0040] Figure 9 Graph of the inhibition rate of hyaluronidase;

[0041] Figure 10 Graph of the ultraviolet resistance test;

[0042] Figure 11 Graph of the blue light resistance test. Detailed implementation method

[0043] The present invention provides a method for extracting organic pigments, comprising the following steps:

[0044] (S1) Pretreat the pitaya peel, then perform drying treatment and pulverization treatment to obtain pitaya peel powder;

[0045] (S2) Mix the pitaya peel powder prepared in step (S1) with water, then perform ultrasonic extraction, and perform post-treatment to obtain organic pigments.

[0046] Further, in step (S1), the pretreatment is to wash the pitaya peel with clean water, then cut off the rhizomes and old and withered parts on the peel, cut the peel into pieces, and then perform freezing treatment.

[0047] Further, in step (S1), during the freezing treatment, the temperature is -20 to -15 °C, and the time is 6 to 8 h;

[0048] The drying treatment is selected from one of hot air drying, spray drying, freeze drying or microwave vacuum drying;

[0049] Preferably, the drying treatment is freeze drying treatment. During the freeze drying treatment, the temperature is -50 to -40 °C, and the time is 24 to 48 h;

[0050] The particle size of the pitaya peel powder is ≤80 mesh.

[0051] Further, in step (S2), the dosage ratio of the pitaya peel powder to water is 1 g: 10 to 50 mL;

[0052] Preferably, the dosage ratio of the pitaya peel powder to water is 1 g: 40 mL.

[0053] Further, in step (S2), during the ultrasonic extraction, the temperature is 15 to 55 °C, the power is 200 to 400 W, and the time is 15 to 55 min;

[0054] Preferably, during the ultrasonic extraction, the temperature is 45 °C, the power is 300 W, and the time is 35 min.

[0055] Further, in step (S2), after the ultrasonic treatment, the obtained ultrasonic extraction liquid is centrifuged, the obtained upper-layer liquid is filtered, and the obtained filtrate is successively subjected to heating and concentration (to avoid ethanol residue caused by ethanol rotary evaporation) and freeze-drying.

[0056] Further, during the centrifugation process, the rotation speed is 7000 - 9000 r / min, and the time is 5 - 15 min;

[0057] Preferably, during the centrifugation process, the rotation speed is 8000 r / min, and the time is 10 min;

[0058] During the filtration process, the pore size of the filter membrane is 40 - 80 μm;

[0059] Preferably, during the filtration process, the pore size of the filter membrane is 40 μm;

[0060] During the heating and concentration process, the temperature is 40 - 60 °C, and the time is 6 - 8 h;

[0061] Preferably, during the heating and concentration process, the temperature is 55 °C, the time is 6 h, and the concentration multiple is 5 - 40 times (as long as it can be concentrated to 1 / 5 - 1 / 40 of the filtrate volume);

[0062] During the freeze-drying process, the temperature is -50 - -40 °C, and the time is 24 - 48 h;

[0063] Preferably, during the freeze-drying process, the temperature is -50 °C, and the time is 48 h.

[0064] The present invention provides an organic pigment obtained by the above method. This organic pigment has excellent antioxidant and anti-inflammatory properties, and also has ultraviolet resistance (280 - 400 nm) and blue light resistance (400 - 500 nm).

[0065] The present invention provides an application of the organic pigment in the preparation of cosmetics (such as blush products or lip cosmetics) and / or foods, or in the field of clothing dyeing.

[0066] The present invention will be described in detail below with reference to the accompanying drawings and specific embodiments.

[0067] In the following examples, unless otherwise specified, the reagents used are commercially available reagents, and the detection means and methods used are conventional detection means and methods in the art.

[0068] Example 1

[0069] This example provides a method for extracting an organic pigment, including the following steps:

[0070] (S1) Take several pitayas, remove the flesh and keep the peel. Wash the peel with clear water, then cut off the rhizomes and old and withered parts on the peel as impurities. Cut the peel into pieces and freeze at -20°C; after freezing for 12 hours, further place it in a freeze dryer for freeze-drying for 48 hours. After taking it out, use a machine to grind it into powder, pass through an 80-mesh sieve to obtain pitaya peel powder, and store it at -20°C.

[0071] (S2) Uniformly mix and dissolve 1 g of pitaya peel powder and 40 mL of water, and perform extraction in an ultrasonic extraction device with a power of 300 W by heating in a water bath at 55°C for 35 minutes; after ultrasonic treatment, centrifuge at a speed of 8000 r / min for 10 minutes, filter the upper liquid, and the obtained filtrate is the pitaya peel stock solution.

[0072] Drawing of the betacyanin standard curve:

[0073] Take betacyanin standard product, use acetic acid-sodium acetate buffer solution with pH 5.4 (mix 14 mL of acetic acid and 86 mL of sodium acetate solution and adjust to pH 5.4), prepare betacyanin with different concentrations, measure the absorbance of betacyanin solutions with different concentrations at 538 nm. Take the absorbance value of the betacyanin mass concentration at 538 nm as the dependent variable and the betacyanin mass concentration as the independent variable to obtain the linear regression equation (as Figure 1 shown).

[0074] Determination of the betacyanin content in the pitaya peel stock solution:

[0075] After placing the pitaya peel stock solution in the dark for 30 minutes, measure the absorbance of the solution at 538 nm. According to the betacyanin standard curve, obtain the yield of betacyanin in the pitaya peel stock solution.

[0076] (S3) Heat and concentrate the filtrate prepared in step (S2) to 5% of the original filtrate volume, and then perform freeze-drying (until the mass remains unchanged) to obtain pitaya peel organic pigment powder: betacyanin.

[0077] Single-factor experiment

[0078] (1) Effect of ultrasonic temperature on the extraction rate of betacyanin in the pitaya peel stock solution

[0079] Set the material-liquid ratio to 1:50 g / mL, the ultrasonic power to 200 W, and the time to 15 minutes; study the effect of different ultrasonic temperatures of 15°C, 25°C, 35°C, 45°C, and 55°C on the extraction rate of betacyanin in the pitaya peel stock solution, with 3 parallels; the results are as Figure 2 shown.

[0080] From Figure 2It can be seen that when the temperature of ultrasound is controlled at 35°C, it is the optimal extraction temperature for betacyanin, and the maximum yield of betacyanin in the pitaya peel liquid is 16.7 mg.

[0081] (2) Effect of solid-liquid ratio on the extraction rate of betacyanin from pitaya peel liquid

[0082] Set the temperature of ultrasound at 35°C, the ultrasound power at 200 W, and the time at 15 min; study the effect of different solid-liquid ratios of 1:10, 1:20, 1:30, 1:40, 1:50 (g:mL) on the extraction rate of betacyanin from pitaya peel liquid, with 3 parallel replicates; the results are as Figure 3 shown.

[0083] From Figure 3 it can be seen that the solid-liquid ratio for the maximum yield of betacyanin in the pitaya peel liquid is 1:40 (g:mL); at this time, the maximum yield of betacyanin in the pitaya peel liquid is 22.61 mg; therefore, the solid-liquid ratio of 1:20 (g:mL) is the optimal extraction ratio for betacyanin.

[0084] (3) Effect of ultrasound time on the extraction rate of betacyanin from pitaya peel liquid

[0085] Set the temperature of ultrasound at 35°C, the solid-liquid ratio at 1:40 (g:mL), and the ultrasound power at 200 W; study the effect of different ultrasound times of 15 min, 25 min, 35 min, 45 min, 55 min on the extraction rate of betacyanin from pitaya peel liquid, with 3 parallel replicates; the results are as Figure 4 shown.

[0086] From Figure 4 it can be seen that as the ultrasound time of the constant-temperature hot water increases, the yield of betacyanin in the pitaya peel liquid gradually increases; when the extraction time is 35 min, the yield of betacyanin in the pitaya peel liquid is the largest, and the maximum yield of betacyanin in the pitaya peel liquid is 23.60 mg; after that, the yield of betacyanin decreases as the ultrasound time increases. Therefore, the optimal ultrasound extraction time for betacyanin is 35 min.

[0087] (4) Effect of ultrasound power on the extraction rate of betacyanin from pitaya peel liquid

[0088] Set the temperature of ultrasound at 35°C, the solid-liquid ratio at 1:40 (g:mL), and the ultrasound time at 35 min; study the effect of different ultrasound powers of 200 W, 250 W, 300 W, 350 W, 400 W on the extraction rate of betacyanin from pitaya peel liquid, with 3 parallel replicates; the results are as Figure 5 shown.

[0089] From Figure 5It can be seen that with the increase of ultrasonic power, the yield of betacyanin increases. The maximum yield of betacyanin in the pitaya peel liquid is 66.91 mg, which is the largest when the ultrasonic power is 300 W. Subsequently, with the increase of ultrasonic power, the yield of betacyanin decreases. It can be analyzed that with the increase of ultrasonic power, the relative content of the dissolved betacyanin increases, and the yield increases. If the ultrasonic power is too high, the internal structure of betacyanin will be damaged, and the relative content of betacyanin will decrease, resulting in a decrease in the yield. Therefore, it is analyzed that 300 W is the optimal ultrasonic extraction power for betacyanin. From Figure 5 it can be seen that the ultrasonic power has a significant effect on the yield of betacyanin.

[0090] In principle, the process should be: ultrasonic extraction temperature 35°C, ultrasonic extraction power 300 W, ultrasonic extraction time 35 min.

[0091] Example 2

[0092] This example provides a method for extracting organic pigments, including the following steps:

[0093] (S1) Take several pitayas, remove the flesh and take the peel. Clean the peel with clear water, then cut off the rhizomes and old and withered parts on the peel and other impurities, cut the peel into pieces and freeze at -20°C; place the pitaya peel frozen for 12 hours in a freeze dryer for freeze-drying for 48 h, use a machine to grind the powder, pass through an 80-mesh sieve to obtain powdery pitaya peel powder, and store it at -20°C.

[0094] (S2) Take 1 g of pitaya peel powder and 40 mL of water, mix and dissolve them evenly, and extract them in an ultrasonic extraction device by means of a water bath; among them, the ultrasonic temperature is 35°C, the ultrasonic power is 300 W, and the ultrasonic time is 35 min; after ultrasonic treatment, centrifuge at a speed of 8000 r / min for 10 minutes, filter the upper liquid, concentrate the obtained filtrate by heating to 5% of the original filtrate volume, and then perform freeze-drying (until the mass remains unchanged) to obtain pitaya peel organic pigment powder: betacyanin.

[0095] Using the method of Example 1, the yield of betacyanin in the pitaya peel liquid obtained in this example is measured to be 4.57%.

[0096] Dissolve the pitaya peel organic pigment powder with water to obtain betacyanin solutions with concentrations of 0.2, 0.4, 0.8, 1.6, and 3.2 mg / mL respectively.

[0097] Performance analysis

[0098] (1) Determination of antioxidant properties:

[0099] 1) Determination of DPPH free radical scavenging rate

[0100] a. Weigh 2 mg of DPPH powder into a 50 mL volumetric flask, make up the volume with absolute ethanol, and sonicate for 5 min to completely dissolve it. After complete dissolution, adjust the absorbance to between 1.2 and 1.3 at 517 nm and store it in the dark.

[0101] b. Pipette 2 mL of betacyanin solutions with different concentrations and 2 mL of VC solution with the same concentration into test tubes. Set VC as the positive control, add samples according to Table 1, react for 120 min in the dark, measure the absorbance at 517 nm, and calculate the DPPH scavenging rate by the following formula:

[0102] Scavenging rate of DPPH·(%) = 1 - (A s - A b ) / A c × 100%

[0103] In the formula, A s : Absorbance of the sample group at 517 nm; A b : Absorbance of the blank group at 517 nm; A c : Absorbance of the control group at 517 nm.

[0104] Table 1 DPPH free radical scavenging experiment

[0105]

[0106] The results are as Figure 6 shown. It can be seen through Figure 6 : When the concentration of the betacyanin solution is 0.2 - 3.2 mg / mL, as the concentration of the betacyanin solution gradually increases, the scavenging rate of DPPH increases relatively fast. When the concentration of the betacyanin solution is equal to 3.2 mg / mL, the efficiency of the betacyanin solution in scavenging DPPH free radicals is the highest, reaching 99.20%; VC is a potent antioxidant that can directly scavenge reactive oxygen species in the body. When the concentration of the betacyanin solution reaches 3.2 mg / mL, it is comparable to the positive control VC and has a good antioxidant effect.

[0107] 2) Determination of ABTS free radical scavenging rate

[0108] Prepare the cation working solution of ABTS free radicals by mixing the ABTS solution with a concentration of 7 mmol / L and the potassium persulfate solution with a concentration of 245 mmol / L (1:1) (store in the dark for 12 h), (pipette 25 drops and add them to a 50 mL centrifuge tube. Then dilute with deionized water to an absorbance of 0.7 ± 0.02).

[0109] Accurately pipette 0.25 mL of sample solutions with different mass concentrations and 1.0 mL of the cation working solution of ABTS free radicals. As shown in Table 2, after mixing, let it stand still, and react for 6 min under the condition of 23 °C (placed in the dark at room temperature). Then measure its absorbance at a wavelength of 734 nm, using distilled water as the blank control, calculate the ABTS free radical scavenging rate (%), and use Vc as the positive control.

[0110] ABTS free radical scavenging rate = [1 - (A1 - A2 / A3)] × 100%

[0111] In the formula:

[0112] A1: Absorbance value of the sample solution and ABTS;

[0113] A2: Absorbance value of the sample solution and distilled water;

[0114] A3: Absorbance value of ABTS without the sample solution

[0115] Table 2 ABTS free radical scavenging experiment

[0116]

[0117]

[0118] The results are as Figure 7 shown. It can be seen through Figure 7 that when the concentration of the betacyanin solution is 0.2 - 3.2 mg / mL, as the concentration of the betacyanin solution gradually increases, the scavenging rate of ABTS increases relatively fast. When the concentration of the betacyanin solution is equal to 3.2 mg / mL, the efficiency of the betacyanin solution in scavenging ABTS free radicals reaches 95.24%. VC is a powerful antioxidant that can directly scavenge reactive oxygen species in the body. When the concentration of the betacyanin solution reaches 3.2 mg / mL, it is comparable to the positive control VC and has a good antioxidant effect.

[0119] 3) Determination of hydroxyl radical scavenging rate

[0120] Accurately weigh different concentrations of betacyanin solutions and the same concentration of VC solution into test tubes according to Table 3. Then, successively add 2 mmol / L FeSO4 solution, 6 mmol / L salicylic acid solution and deionized water into the test tubes and shake well. Add 1 mmol / L H2O2 to start the reaction, place it in a constant temperature incubator at 37 °C for 30 min. After taking it out, measure the absorbance at 510 nm with an enzyme-linked immunosorbent assay instrument, and calculate the hydroxyl radical scavenging ability of the betacyanin solution. Among them, VC is the positive control, and three parallel experiments are carried out for each group.

[0121] The hydroxyl radical scavenging ability of the betacyanin solution is calculated using the following formula:

[0122] Scavenging rate of ·OH radicals = 1 - [(A1 - A2) / A3] × 100%

[0123] In the formula:

[0124] A1: Absorbance of the sample and hydroxyl groups added

[0125] A2: Absorbance of the sample and water

[0126] A3: Absorbance without adding the sample

[0127] Table 3 Hydroxyl radical scavenging experiment

[0128]

[0129] The results are as Figure 8 shown. It can be seen through Figure 8 that when the concentration of betacyanin solution is 0.2 - 3.2 mg / mL, the hydroxyl radical scavenging rate increases with the increase of the mass concentration of betacyanin. When the concentration of betacyanin solution reaches 3.2 mg / mL, the hydroxyl radical scavenging rate of betacyanin solution reaches 94.63%. VC is a potent antioxidant that can directly scavenge reactive oxygen species in the body. When the concentration of betacyanin solution reaches 3.2 mg / mL, it is comparable to the positive control VC, so it has good antioxidant effects.

[0130] (2) Determination of anti-inflammatory - hyaluronidase inhibition rate:

[0131] Prepare 0.1M glacial acetic acid solution, 0.1M sodium acetate solution, 0.5M sodium carbonate solution, 10M hydrochloric acid solution, 0.1M acetate buffer solution, 4000U / mL hyaluronidase solution, 12.5mM calcium chloride solution, sodium hyaluronate solution, 0.4M sodium hydroxide solution, acetylacetone solution, and p-dimethylaminobenzaldehyde solution for standby.

[0132] Add reagents according to Table 4. The sample group is betacyanin solution and dipotassium glycyrrhizinate solution (DPG). Dipotassium glycyrrhizinate solution is used as a positive control for the inhibitory effect on hyaluronidase activity. After the reaction, measure its absorbance at 585 nm with an enzyme-labeled instrument, and calculate the inhibitory ability of betacyanin solution on hyaluronidase activity. Among them, three parallel experiments are set for each group.

[0133] The inhibition rate of betacyanin on hyaluronidase can reflect the strength of its anti-inflammatory activity. The higher the inhibition rate, the stronger the anti-inflammatory activity. Calculate the inhibition rate of betacyanin solution on hyaluronidase activity according to the following formula:

[0134]

[0135] Where: T is the OD of the sample tube; T0 is the OD of the sample control tube; C is the OD of the negative control tube; C0 is the OD of the blank control tube.

[0136] Table 4 Inhibitory Experiment of Hyaluronidase Activity

[0137]

[0138]

[0139] The results are as Figure 9 shown. It can be seen from Figure 9 that when the concentration of the betacyanin solution is 0.2 - 3.6 mg / mL, with the increase of the concentration of the betacyanin solution, the inhibitory intensity of its activity on hyaluronidase gradually increases. When the concentration of the betacyanin solution is 3.2 mg / mL, the inhibition rate of the betacyanin solution on hyaluronidase activity reaches 91.53%. It shows that the betacyanin solution has a good inhibitory effect on hyaluronidase.

[0140] (3) Determination of Anti-ultraviolet Ability and Anti-blue Light Ability

[0141] The ultraviolet spectrophotometry was used to measure the transmittance of betacyanin solutions with different concentrations in the wavelength range of 280 - 400 nm to evaluate the sunscreen performance of betacyanin and the transmittance in the wavelength range of 400 - 500 nm to evaluate the anti-blue light performance of betacyanin

[0142] Anti-ultraviolet ability: Measure the transmittance of the sample at 280, 290, 300, 310, and 320 nm in the UVB region and the transmittance at 320, 330, 340, 350, 360, 370, 380, 390, and 400 nm in the UVA region

[0143] Ultraviolet absorption rate formula: Ultraviolet absorption rate / % = (1 - transmittance) × 100%

[0144] The results are as Figure 10 shown. It can be seen through Figure 10 that when the concentration of the betacyanin solution is 0.25 - 4.0 mg / mL, with the increase of the concentration of the betacyanin solution, its absorption rate in the wavelength range of 280 - 400 nm gradually increases, and the absorption rate at the same concentration decreases with the increase of the wave number. As Figure 10 shown, when the concentration of the betacyanin solution is equal to 4.0 mg / mL, the anti-ultraviolet effect of the betacyanin solution is the best, with an average absorption rate of 90% in the UVB region and 70% in the UVA region.

[0145] Anti-blue light ability: Measure the transmittance of the sample at 400, 410, 420, 430, 440, 450, 460, 470, 480, 490, and 500 nm in the blue light region.

[0146] Blue light blocking calculation formula: Blue light absorption rate / % = (1 - transmittance) × 100%

[0147] The results are as Figure 11 shown. Through Figure 11 it can be seen that when the concentration of the betacyanin solution is 0.25 - 4.0 mg / mL, as the concentration of the betacyanin solution increases, its absorption rate in the wavelength range of 400 - 500 nm gradually increases. As Figure 11 shown, when the concentration of the betacyanin solution is equal to 4.0 mg / mL, the blue light blocking effect of the betacyanin solution is the best, and the average absorption rate of blue light reaches 80.29%.

[0148] The above description of the embodiments is to enable those of ordinary skill in the art to understand and use the invention. It is obvious that those skilled in the art can easily make various modifications to these embodiments and apply the general principles described herein to other embodiments without creative efforts. Therefore, the present invention is not limited to the above embodiments, and all improvements and modifications made by those skilled in the art without departing from the scope of the present invention according to the interpretation of the present invention should be within the protection scope of the present invention.

Claims

1. A method for extracting an organic pigment, characterized in that, It includes the following steps: (S1) Pretreat the pitaya peel, then carry out drying treatment and pulverization treatment to obtain pitaya peel powder; (S2) Mix the pitaya peel powder prepared in step (S1) with water, carry out ultrasonic extraction, and perform post-treatment to obtain an organic pigment.

2. The extraction method of an organic pigment according to claim 1, characterized in that, In step (S1), the pretreatment is to wash the pitaya peel with clean water, then cut off the rhizomes and old and withered parts on the peel, cut the peel into pieces, and then carry out freezing treatment.

3. The extraction method of an organic pigment according to claim 1, characterized in that, In step (S1), the drying treatment is selected from one of hot air drying, spray drying, freeze drying or microwave vacuum drying; The particle size of the pitaya peel powder is ≤80 mesh.

4. The extraction method of an organic pigment according to claim 1, wherein, In step (S2), the dosage ratio of the pitaya peel powder to water is 1 g: 10 - 50 mL.

5. The extraction method of an organic pigment according to claim 1, characterized in that, In step (S2), during the ultrasonic extraction process, the temperature is 15 - 55 °C, the power is 200 - 400 W, and the time is 15 - 55 min.

6. The extraction method of an organic pigment according to claim 5, characterized in that, During the ultrasonic extraction process, the temperature is 45 °C, the power is 300 W, and the time is 35 min.

7. The extraction method of an organic pigment according to claim 1, characterized in that, In step (S2), the post-treatment is that after the ultrasonic extraction ends, centrifuge the obtained ultrasonic extraction liquid, filter the obtained upper liquid, and sequentially carry out heating concentration and freeze drying on the obtained filtrate.

8. The extraction method of an organic pigment according to claim 7, wherein, During the centrifugation process, the rotation speed is 7000 - 9000 r / min, and the time is 5 - 15 min; During the filtration process, the pore size of the filter membrane is 40 - 80 μm; During the heating concentration process, the temperature is 40 - 60 °C, and the time is 6 - 8 h; During the freeze drying process, the temperature is -50 - -40 °C, and the time is 24 - 48 h.

9. An organic pigment, characterized in that, It is obtained by the method according to any one of claims 1 - 8.

10. An application of the organic pigment according to claim 9 in the preparation of cosmetics and / or foods, or, an application in the field of clothing dyeing.

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