Chlorophyll fluorescence imager for seedling phenotype detection

Abstract

The invention relates to the technical field of chlorophyll fluorescence imaging, in particular to a chlorophyll fluorescence imager for seedling phenotype detection, comprising: a base, the upper end of which is provided with a lifting member; the imaging main body is connected with the movable part of the lifting piece; the trapezoidal cover is connected with the lower end of the imaging body; the rectangular cover is communicated with the lower end of the trapezoidal cover; the movable plate is movably connected with the right wall in the rectangular cover; the first driving part is connected with the right end of the trapezoidal cover; the second driving piece is connected with the left end of the movable plate; the rubber net is connected with the left end of the second driving part; the header pipe is connected with the left end of the movable plate; by means of the design, shells left on seedlings can be mechanically and flexibly removed, the probability that leaves of the seedlings are damaged and the like is effectively reduced, the probability that chlorophyll fluorescence imaging of the seedlings has errors and the like is effectively reduced, and the reliability of chlorophyll fluorescence imaging of the seedlings is improved. The phenotype detection effect and quality of the seedlings are effectively ensured.

Description

Technical Field

[0001] The present invention is a chlorophyll fluorescence imager for seedling phenotype detection, belonging to the technical field of chlorophyll fluorescence imaging technology. Background Art

[0002] The chlorophyll fluorescence phenomenon is of crucial significance in the study of plant photosynthesis. It can not only intuitively reflect the primary reaction processes of photosynthesis such as light energy absorption, excitation energy transfer, and photoreaction, but also be closely related to subsequent core processes such as electron transfer, proton gradient establishment, ATP synthesis, and CO2 fixation. It can be said that the dynamic changes of almost all photosynthesis links can be clearly presented through the chlorophyll fluorescence characteristics. For this reason, chlorophyll fluorescence has become an important probe for photosynthesis research, and photosynthesis efficiency has also become one of the core detection standards in the field of seedling cultivation. In actual detection applications, a chlorophyll fluorescence imager is usually used to achieve precise detection of seedling phenotypes.

[0003] Currently, the mainstream chlorophyll fluorescence imager generally consists of a base, a lifting mechanism arranged on the base, an imager main body connected to the movable part of the lifting mechanism, and a light-shielding outer cover installed on the outer periphery of the lower end of the imager main body. The detection operation process is as follows: First, place the flowerpot with planted seedlings at the designated position on the base, and drive the imager main body and the outer cover to move downward synchronously through the lifting mechanism, so that the seedlings completely enter the detection space formed by the outer cover. Subsequently, the imager main body emits excitation light with a specific wavelength to the seedlings. After the chlorophyll molecules in the seedling leaves absorb light energy, they quickly jump from the ground state to the excited state. Since the excited-state chlorophyll molecules are extremely unstable, they will quickly return to the ground state through ways such as emitting fluorescence. At this time, the imager main body accurately captures the fluorescence signal emitted by the chlorophyll and converts it into a digital signal and transmits it to the corresponding terminal device in real time. And the terminal device calculates chlorophyll fluorescence parameters (such as Fv / Fm, ΦPSII, etc.) through in-depth processing and analysis of the fluorescence data, and generates a visual image based on the parameters to intuitively reflect the photosynthetic physiological state of the plant.

[0004] However, in the actual seedling cultivation process, some seedling leaves are prone to residual seed coats. And the residual shells are likely to form a physical blockage to the subsequent fluorescence imaging detection. Currently, generally, tools such as tweezers are used for manual removal. However, due to the adhesiveness between the seed coat and the seedling leaves, and it is difficult to accurately control the force during manual operation, it is extremely easy to cause damage to the seedling leaves. Statistical data shows that the leaf damage rate caused by manual removal can reach 37%, which will cause deviations in chlorophyll fluorescence imaging data and ultimately affect the effect and quality of seedling phenotype detection. Summary of the Invention

[0005] In view of the problems in the prior art, the present invention provides a chlorophyll fluorescence imager for seedling phenotype detection.

[0006] The technical solution adopted by the present invention to solve its technical problems is as follows: A chlorophyll fluorescence imager for seedling phenotype detection, comprising: A base, on which a lifting member is installed at the upper end; An imaging main body, connected to the movable part of the lifting member; A trapezoidal cover, connected to the lower end of the imaging main body, the imaging main body extends into the trapezoidal cover, and the trapezoidal cover is arranged with a narrow upper part and a wide lower part; A rectangular cover, communicated with the lower end of the trapezoidal cover; A movable plate, movably connected to the inner right wall of the rectangular cover, the movable plate is arranged in a left-high and right-low inclined manner, and the movable plate extends into the trapezoidal cover; A first driving member, connected to the right end of the trapezoidal cover, the movable part of the first driving member passes through the trapezoidal cover and is movably connected to the right end of the movable plate; A second driving member, connected to the left end of the movable plate; A rubber net, connected to the left end of the second driving member; A main pipe, connected to the left end of the movable plate, the main pipe is located above the second driving member and on the right side of the imaging main body; A first atomizing nozzle, a plurality of the first atomizing nozzles are provided, and the plurality of the first atomizing nozzles are equidistantly communicated and arranged at the upper end of the main pipe, and a first control valve is assembled on the first atomizing nozzle; A second atomizing nozzle, a plurality of the second atomizing nozzles are provided, and the plurality of the second atomizing nozzles are equidistantly communicated and installed at the lower left end of the main pipe, and a second control valve is assembled on the second atomizing nozzle; An air drying member, connected to the left end of the rectangular cover, the air drying member extends into the rectangular cover and is located on the left side of the imaging main body.

[0007] Furthermore, the air drying member includes a cylinder, the cylinder is slidably connected to the left end of the rectangular cover and extends into the rectangular cover, a conical cylinder is communicated with the left end of the cylinder, and the conical cylinder is arranged with a narrow left part and a wide right part. A connecting pipe is communicated and installed at the left end of the conical cylinder, the other end of the connecting pipe is communicated with a first extraction device, the first extraction device is arranged on the upper end of the base and behind the lifting member, a spiral plate is installed in the cylinder, and the lower end surface of the cylinder is recessed upward to form an opening, and the opening extends to the inner wall of the cylinder; Fixing plates are installed at both the front and rear ends of the cylinder, and the fixing plates are located in the rectangular cover. A telescopic device is arranged at the left end of the rectangular cover and behind the cylinder, and a first telescopic rod is installed at the left end of the rectangular cover and in front of the cylinder. The movable parts of the telescopic device and the first telescopic rod both pass through the rectangular cover and are respectively connected to the left ends of the two fixing plates.

[0008] Furthermore, an air pipe is installed at the upper end of the cylinder, and the right end of the air pipe extends into the rectangular cover. A plurality of suction pipes are equidistantly and communicatively arranged at the lower end of the air pipe, and the lower ends of the suction pipes penetrate through the cylinder and the opening. The left end of the air pipe is communicatively arranged with the inlet part of the first extraction device. A cleaning component is installed at the upper end of the air pipe, and the upper end of the cleaning component extends into the trapezoidal cover and fits with the inner wall of the trapezoidal cover.

[0009] Furthermore, the cleaning component includes a mounting seat arranged at the upper end of the air pipe. A trapezoidal block is arranged directly above the mounting seat, and the trapezoidal block is arranged with a narrow upper part and a wide lower part. The inclined surface of the trapezoidal block fits with the inner left wall of the trapezoidal cover. Two second elastic members are symmetrically installed between the mounting seat and the trapezoidal block; Two second telescopic rods are symmetrically arranged between the mounting seat and the trapezoidal block, and the two second telescopic rods are respectively located inside the two second elastic members. An installation plate is arranged at the right end of the trapezoidal block, and a cleaning strip is installed at the upper end of the installation plate, and the upper end of the cleaning strip fits with the inner wall of the trapezoidal cover in an extrusion manner.

[0010] Furthermore, the second driving member includes a mounting frame. A rubber net is arranged inside the mounting frame, and the mounting frame is located on the left side of the movable plate and under the main pipe. T-shaped rods are installed at the four corner positions at the right end of the mounting frame. The rod parts of the four T-shaped rods all penetrate through the movable plate, and the T-shaped rods are slidably connected with the movable plate; Third elastic members are arranged at the four corner positions at the right end of the movable plate, and the other ends of the four third elastic members are respectively connected with the left ends of the plate parts of the four T-shaped rods, and the four third elastic members are respectively located outside the rod parts of the four T-shaped rods. Two magnetic strips are symmetrically arranged at the right end of the mounting frame, and the two magnetic strips are located on the upper and lower sides of the rubber net. Two electromagnets are symmetrically installed at the left end of the movable plate, and the two electromagnets are respectively located directly to the right of the two magnetic strips. The electromagnets and the magnetic strips are arranged to repel each other, and the electromagnets and the magnetic strips are both located outside the T-shaped rods.

[0011] Furthermore, the first driving member includes a lifting device installed on the upper right end of the trapezoidal cover. A slider is slidably connected to the right end of the movable plate, and the slider is located outside the T-shaped rod. The movable part of the lifting device passes through the trapezoidal cover and is movably connected with the slider.

[0012] Furthermore, the lifting member includes an installation box arranged on the upper end of the base. A lead screw is rotatably connected inside the installation box. A driving device is installed at the upper end of the installation box, and the output shaft of the driving device is connected with the lead screw. The outer end of the lead screw is connected with a movable block through a ball screw nut pair. The movable block is slidably connected inside the installation box. The movable block extends out of the front side of the installation box and is connected with the imaging main body. Two guide rods are symmetrically installed inside the installation box, and the two guide rods are located on the left and right sides of the lead screw. The two guide rods both penetrate through the movable block, and the movable block is slidably connected with the guide rods.

[0013] Furthermore, a concave hole is formed by the downward depression of the upper end surface of the base, and the concave hole is located directly below the rectangular cover. A second extraction device is provided at the upper end of the movable block, and the second extraction device is located at the front side of the installation box. The rear end of the main pipe is connected to the second extraction device through a hose.

[0014] Advantages of the present invention: By using the second electric push rod and the slider, the components such as the movable plate, the main pipe, and the installation frame are rotated to a horizontal state. At the same time, by using the water pump, multiple second control valves, the main pipe, and multiple second atomizing nozzles, atomized warm water is sprayed on the seedlings, so as to soften the residual shells on the seedlings and weaken the adhesion degree. Then, by using the electromagnet, the magnetic strip, four T-shaped rods, and four third elastic members, the installation frame and the rubber net move up and down, so as to flexibly pat the top of the seedlings with the rubber net, and then the shells on the seedlings fall off, realizing the mechanical and flexible removal of the residual shells on the seedlings, effectively reducing the probability of damage to the leaves of the seedlings due to factors such as manual removal, effectively reducing the probability of errors in the chlorophyll fluorescence imaging of the seedlings, and effectively ensuring the effect and quality of the phenotypic detection of the seedlings. Description of the Drawings

[0015] By reading the detailed description of the non-limiting embodiments with reference to the following drawings, other features, purposes, and advantages of the present invention will become more obvious: Figure 1 It is a schematic structural diagram of a chlorophyll fluorescence imager for seedling phenotypic detection according to the present invention; Figure 2 It is a three-dimensional view of a chlorophyll fluorescence imager for seedling phenotypic detection according to the present invention; Figure 3 It is a sectional view of a chlorophyll fluorescence imager for seedling phenotypic detection according to the present invention; Figure 4 It is Figure 3 The enlarged view of part A in Figure 5 It is Figure 3 The enlarged view of part B in Figure 6 It is Figure 3 The enlarged view of part C in Figure 7 It is a three-dimensional view of the installation box in a chlorophyll fluorescence imager for seedling phenotypic detection according to the present invention; Figure 8 It is an assembly diagram of the rubber net and the movable plate in a chlorophyll fluorescence imager for seedling phenotypic detection according to the present invention; Figure 9 It is a three-dimensional view of the cylinder in a chlorophyll fluorescence imager for seedling phenotypic detection according to the present invention; Figure 10 Stereogram of the trachea in a chlorophyll fluorescence imager for seedling phenotype detection according to the present invention; Figure 11 Schematic diagram of another embodiment of a chlorophyll fluorescence imager for seedling phenotype detection according to the present invention; Figure 12 Assembly drawing of the circular plate and the metal tube in a chlorophyll fluorescence imager for seedling phenotype detection according to the present invention.

[0016] In the figure: 1, base, 11, concave hole, 12, air pump, 2, installation box, 21, lead screw, 22, motor, 23, guide rod, 3, movable block, 31, water pump, 32, circular plate, 33, first elastic member, 34, metal tube; 4, imaging main body, 5, first electric push rod, 51, first telescopic rod, 6, cylinder, 61, connecting pipe, 62, spiral plate, 63, opening, 64, fixing plate, 7, trachea, 71, suction pipe, 72, mounting seat, 73, second telescopic rod, 74, second elastic member, 75, trapezoidal block, 76, mounting plate, 77, sponge strip, 8, rectangular cover, 81, trapezoidal cover; 9, second electric push rod, 91, rubber net, 92, movable plate, 93, slider, 94, T-shaped rod, 95, third elastic member, 96, main pipe, 961, first atomizing nozzle, 962, second atomizing nozzle, 97, mounting frame, 98, magnetic strip, 99, electromagnet. Detailed implementation manners

[0017] To make the technical means, creative features, achieved purposes and functions of the present invention easy to understand, the present invention will be further described below in conjunction with specific implementation manners.

[0018] Embodiment 1: As shown in Figure 1 , Figure 2 , Figure 3 , Figure 6 , Figure 7 and Figure 8 shown, a chlorophyll fluorescence imager for seedling phenotype detection is provided, including: a base 1, an installation box 2 is arranged on the upper end of the base 1. Through the installation box 2, an installation space is provided for components such as the lead screw 21, and the lead screw 21 is rotatably connected in the installation box 2. Through the lead screw 21, the movable block 3 moves up and down. Then, the fixed part of the driving device with the output shaft connected to the lead screw 21 is installed on the upper end of the installation box 2. Through the driving device, the lead screw 21 is driven to rotate. The driving device can adopt the motor 22, and the movable block 3 that is slidably connected in the installation box 2 and extends out of the front side of the installation box 2 is arranged on the outer end of the lead screw 21 through a ball screw pair; The imaging main body 4 extending into the trapezoidal cover 81 is installed on the front end of the movable block 3. Through the movable block 3, an installation carrier is provided for the imaging main body 4. Two guide rods 23 that penetrate the movable block 3 and are slidably connected to the movable block 3 on the left and right sides of the lead screw 21 are symmetrically installed in the installation box 2. The two guide rods 23 are used in cooperation to guide the movement of the movable block 3. A concave hole 11 located directly below the rectangular cover 8 is formed by recessing downward on the upper end surface of the base 1. Through the concave hole 11, the flower pot is positioned and placed.

[0019] The trapezoidal cover 81 arranged with a narrow upper part and a wide lower part is set at the lower end of the imaging main body 4. Through the trapezoidal cover 81, an installation carrier is provided for the rectangular cover 8. The rectangular cover 8 is communicatively arranged at the lower end of the trapezoidal cover 81. Through the rectangular cover 8, an installation space is provided for movable components and the like. Then, the movable plate 92 arranged in a left-high and right-low inclined manner and extending into the trapezoidal cover 81 is movably installed on the inner right wall of the rectangular cover 8. Through the movable plate 92, an installation carrier is provided for components such as the slider 93. The slider 93 located outside the T-shaped rod 94 is slidably connected to the right end of the movable plate 92. Through the slider 93, the lifting device is connected to the movable plate 92. Then, the fixed part of the lifting device whose movable part passes through the trapezoidal cover 81 and is movably connected to the slider 93 is installed on the right end of the trapezoidal cover 81. Through the lifting device, the slider 93 and the movable plate 92 are driven to move. The lifting device can adopt the second electric push rod 9.

[0020] The rubber net 91 is installed in the installation frame 97, and the installation frame 97 is located on the left side of the movable plate 92 and below the main pipe 96. Through the installation frame 97, an installation carrier is provided for components such as the rubber net 91. The rod parts of the four T-shaped rods 94 whose rod parts penetrate the movable plate 92 and are slidably connected to the movable plate 92 are respectively installed at the four corner positions on the right end of the installation frame 97. The four T-shaped rods 94 are used in cooperation to connect the installation frame 97 and the movable plate 92. Then, four third elastic members 95 respectively located outside the rod parts of the four T-shaped rods 94 are respectively arranged at the four corner positions on the right end of the movable plate 92, and the other ends of the four third elastic members 95 are respectively connected to the left ends of the plate parts of the four T-shaped rods 94. The four third elastic members 95 are used in cooperation to make the installation frame 97 return to its original position. The third elastic member 95 can adopt a spring; Two magnetic strips 98 located outside the T-shaped rod 94 on the upper and lower sides of the rubber net 91 are symmetrically arranged on the right end of the installation frame 97. Two electromagnets 99 located outside the T-shaped rod 94 and arranged in mutual repulsion with the magnetic strips 98 are symmetrically installed on the left end of the movable plate 92. The electromagnets 99 and the magnetic strips 98 are used in cooperation to make the installation frame 97 move to the left. Then, the main pipe 96 located above the installation frame 97 and on the right side of the imaging main body 4 is installed on the left end of the movable plate 92. Through the main pipe 96, an installation carrier is provided for the second atomizing nozzle 962; A plurality of second atomizing nozzles 962 equipped with second control valves are equidistantly connected and installed on the lower left end of the main pipe 96. The plurality of second atomizing nozzles 962 are used in combination to spray warm water on the seedlings. A second extraction device arranged at the upper end of the movable block 3 and connected to the rear end of the main pipe 96 through a hose and located on the front side of the installation box 2 is provided, and the water inlet part of the second extraction device is communicated with the warm water source. Through the second extraction device, warm water is conveyed into the main pipe 96. The second extraction device can adopt a water pump 31.

[0021] Before use, first measure the height of the seedlings and transmit the measurement data to the terminal. Then place the flower pot with the seedlings on the concave hole 11, so as to position and place the flower pot on the base 1. At this time, the seedlings are directly below the imaging body 4. Then start the motor 22 to drive the screw rod 21 to rotate. Since the screw rod 21 is connected to the movable block 3 through a ball screw pair, the rotation of the screw rod 21 will cause the movable block 3 to move downward, so that the imaging body 4, the trapezoidal cover 81 and the rectangular cover 8 move downward to a predetermined position. At this time, the seedlings are located inside the rectangular cover 8; Then start the second electric push rod 9 to drive the slider 93 to move, and then make the movable plate 92 rotate, so that components such as the main pipe 96 and the installation frame 97 rotate, and make the movable plate 92 rotate to a horizontal state. And when the movable plate 92 rotates to a predetermined angle, start the water pump 31 and a plurality of second control valves to extract the warm water in the warm water source, then convey the extracted warm water into the main pipe 96, and then the water in the main pipe 96 is distributed to a plurality of second atomizing nozzles 962, and the warm water is atomized and sprayed through the plurality of second atomizing nozzles 962, so that the atomized warm water is sprayed on the seedlings. The temperature of the warm water is 30-35°C, and the water quality is selected as distilled water or filtered water, effectively avoiding stimulating the roots of the seedlings and ensuring the normal growth of the subsequent seedlings; At this time, the atomized warm water is also sprayed on the remaining shells on the leaves of the seedlings, so that the fibers on the shells absorb water and expand. With the assistance of temperature, the hardness and toughness of the shells are both reduced and softened, and at the same time, the adhesion between the shells and the leaves of the seedlings is weakened; Then connect the circuit of the electromagnet 99. Since the electromagnet 99 and the magnetic strip 98 are arranged to repel each other, a repulsive force is generated between the electromagnet 99 and the magnetic strip 98 when the circuit is connected. Under the action of the repulsive force, the magnetic strip 98, the installation frame 97 and the rubber net 91 move downward, and then the rubber net 91 contacts the top of the seedlings, and the four T-shaped rods 94 move downward, so as to compress the third elastic member 95 and make the third elastic member 95 generate an elastic force. Then disconnect the circuit of the electromagnet 99, and under the elastic force of the third elastic member 95, the magnetic strip 98, the installation frame 97 and the rubber net 91 move upward and return to their original positions. Then perform the above steps in a cycle, so that the installation frame 97 and the rubber net 91 move up and down, and then use the rubber net 91 to gently pat the top of the seedlings; Furthermore, with the assistance of flexible flapping, the upper shell of the seedling is detached, realizing the mechanical and flexible removal of the remaining shell on the seedling, effectively reducing the probability of damage to the leaf part of the seedling due to factors such as manual removal, effectively reducing the probability of errors in the chlorophyll fluorescence imaging of the seedling, and effectively ensuring the effect and quality of the seedling phenotype detection.

[0022] After the upper shell of the seedling leaf is removed, the second electric push rod 9 is used to turn the components such as the movable plate 92 back to their original positions, and then the imaging main body 4 is started. The imaging main body 4 works and irradiates the seedling with light of a specific wavelength, causing the chlorophyll molecules in the seedling to absorb light energy and transition from the ground state to the excited state. The excited chlorophyll molecules are unstable and will return to the ground state in the form of emitting fluorescence, etc. The imaging main body 4 captures the fluorescence signal emitted by the chlorophyll, converts the fluorescence signal into data, and transmits the collected data to the terminal. Then the terminal processes and analyzes the data, calculates the chlorophyll fluorescence parameters, and forms an image based on the chlorophyll fluorescence parameters, thereby displaying the spatial distribution and dynamic changes of chlorophyll fluorescence on the plant surface in the form of an image, reflecting the photosynthetic physiological status of the plant, and then completing the seedling phenotype detection operation.

[0023] Embodiment 2: Through the linkage of the second electric push rod 9, the slider 93 and the movable plate 92, the rubber net 91 and components such as the main pipe 96 are driven to rotate, and the water pump 31 is used to spray atomized warm water on the seedlings positioned on the base 1 through the main pipe 96 and a plurality of second atomizing nozzles 962 to soften the remaining shells on the seedlings. With the cooperation of the magnetic strip 98, the electromagnet 99, the four T-shaped rods 94 and the third elastic member 95, the rubber net 91 generates an up-and-down cyclic movement to perform flexible flapping on the seedlings to complete the shell removal. However, this structure has technical pain points: after the atomized warm water is sprayed, water droplets are likely to remain on the surface of the seedlings, resulting in interference artifacts in the subsequent chlorophyll fluorescence imaging; and the lens of the imaging main body 4 is exposed when idle, and is easily attached with dust and other impurities. Generally, before work, tools such as a wet cloth are used, and under the block of components such as the rectangular cover 8, the lens of the imaging main body 4 is manually wiped and cleaned in a hidden manner, but such an operation increases the risk of scratching the lens, and is more likely to cause light spots during imaging due to wiping marks, ultimately affecting the detection accuracy of the chlorophyll fluorescence imaging of the seedlings.

[0024] To solve the above problems, as Figure 1 、 Figure 2 、 Figure 3 、 Figure 4 、 Figure 5 、 Figure 6 、 Figure 9 and Figure 10As shown in the figure, a plurality of first atomizing nozzles 961 equipped with first control valves are equidistantly connected to the upper end of the main pipe 96. The plurality of first atomizing nozzles 961 are used in combination to spray water on the lens surface at the lower end of the imaging body 4. The cylinder 6 extending into the rectangular cover 8 and located on the left side of the imaging body 4 is slidably connected to the left end of the rectangular cover 8. Through the cylinder 6, an installation carrier is provided for components such as the conical cylinder. Then, the conical cylinder arranged with a narrow left end and a wide right end is connected to the left end of the cylinder 6. Through the conical cylinder, the connecting pipe 61 is connected between the cylinder 6; The first extraction device located at the rear end of the installation box 2 is arranged on the upper end of the base 1. Through the first extraction device, it is used to extract gas. The first extraction device can adopt an air pump 12, and the connecting pipe 61 with the other end connected to the outlet part of the first extraction device is connected and installed on the left end of the conical cylinder. Through the connecting pipe 61, the conical cylinder is connected to the first extraction device. Then, the spiral plate 62 is installed in the cylinder 6. Through the spiral plate 62, the inside of the cylinder 6 is separated into a spiral channel. An opening 63 extending to the inner wall of the cylinder 6 is recessed upward on the lower end surface of the cylinder 6. Through the opening 63, gas is sprayed outwards; Two fixing plates 64 located in the rectangular cover 8 are respectively installed at the front and rear ends of the cylinder 6. Through the fixing plates 64, the cylinder 6 moves left and right. The fixed part of the telescopic device located at the rear side of the cylinder 6 is arranged on the left end of the rectangular cover 8. Then, the fixed part of the first telescopic rod 51 located at the front side of the cylinder 6 is installed on the left end of the rectangular cover 8. The movable parts of the telescopic device and the first telescopic rod 51 both pass through the rectangular cover 8 and are respectively connected to the left ends of the two fixing plates 64. Through the telescopic device, the cylinder 6 is driven to move left and right. The telescopic device can adopt a first electric push rod 5, and through the first telescopic rod 51, the movement of the cylinder 6 is guided; The trachea 7 with the right end extending into the rectangular cover 8 is installed on the upper end of the cylinder 6, and the left end of the trachea 7 is connected to the inlet part of the first extraction device. Through the trachea 7, an installation carrier is provided for the straw 71, and a plurality of straws 71 with the lower ends penetrating through the cylinder 6 and the opening 63 are equidistantly connected to the lower end of the trachea 7. The plurality of straws 71 are used in combination to extract gas from the position below the opening 63; The mounting seat 72 is arranged on the upper end of the trachea 7, and the inclined surface of the trapezoidal block 75 arranged with a narrow upper end and a wide lower end and located directly above the mounting seat 72 is mutually attached to the inner left wall of the trapezoidal cover 81. The mounting seat 72 and the trapezoidal block 75 are used in combination to provide an installation carrier for components such as the second telescopic rod 73. Then, two second elastic members 74 are symmetrically installed between the mounting seat 72 and the trapezoidal block 75. The two second elastic members 74 are used in combination to apply an upward thrust to the trapezoidal block 75. The second elastic member 74 can adopt a spring; The two second telescopic rods 73 respectively located inside the two second elastic members 74 are symmetrically arranged between the mounting seat 72 and the trapezoidal block 75. The two second telescopic rods 73 are used in cooperation to guide the movement of the trapezoidal block 75, and the mounting plate 76 is arranged on the right end of the trapezoidal block 75. Through the mounting plate 76, a mounting carrier is provided for the cleaning strip. Then, the cleaning strip with its upper end in extrusion fit with the inner wall of the trapezoidal cover 81 is mounted on the upper end of the mounting plate 76. Through the cleaning strip, the lens surface of the imaging main body 4 is cleaned. The cleaning strip can be a sponge strip 77.

[0025] During use, first use the concave hole 11 to position and place the flowerpot planted with seedlings on the base 1. Then, use the motor 22, the lead screw 21 and the movable block 3 to move the imaging main body 4, the trapezoidal cover 81 and the rectangular cover 8 downward to a predetermined position. Then, through the second electric push rod 9 and the slider 93, rotate the movable plate 92, the main pipe 96, the mounting frame 97 and other components to the horizontal state. At the same time, use the water pump 31, the main pipe 96 and a plurality of second atomizing nozzles 962 to atomize and spray warm water onto the seedlings, thereby softening the residual shell on the seedlings by using the atomized warm water, and at the same time weakening the adhesion between the shell and the leaf part of the seedlings; At the same time, start a plurality of first control valves. Then, the warm water in the main pipe 96 is branched to a plurality of first atomizing nozzles 961, and the first atomizing nozzles 961 are used to spray the atomized warm water outward. The first atomizing nozzles 961 are in a rotating state, so that the atomized warm water is sprayed onto the lower end of the lens surface of the imaging main body 4. When the movable plate 92 rotates to a predetermined angle, the first control valve is closed to effectively avoid excessive waste of warm water; After the movable plate 92 rotates to the horizontal state, then connect the circuit of the electromagnet 99, so that a repulsive force is generated between the electromagnet 99 and the magnetic strip 98. Under the action of the repulsive force and with the assistance of the four T-shaped rods 94, the magnetic strip 98, the mounting frame 97 and the rubber net 91 move downward, so that the rubber net 91 comes into contact with the top of the seedlings. Then, under the elastic force of the third elastic member 95, the magnetic strip 98, the mounting frame 97 and the rubber net 91 move upward and return to their original positions. Then, the mounting frame 97 and the rubber net 91 move up and down, so as to flexibly pat the top of the seedlings by using the rubber net 91, and thus the shell on the seedlings falls off.

[0026] After the shell on the leaf part of the seedlings is removed, then use the second electric push rod 9 to rotate the movable plate 92 and other components back to their original positions. Then, use the motor 22 and the lead screw 21 to adjust the height of the rectangular cover 8 and other components. Then, start the first electric push rod 5, so that with the assistance of the first telescopic rod 51, the two fixing plates 64 move to the right, and thus the cylinder 6 and the air pipe 7 move to the right; During the rightward movement of the trachea 7, the mounting base 72, the second telescopic rod 73, the trapezoidal block 75, the mounting plate 76, and the sponge strip 77 will move to the right. At this time, the second elastic member 74 is in a compressed state and has elastic force. Thus, under the action of the elastic force of the second elastic member 74, the trapezoidal block 75, the mounting plate 76, and the sponge strip 77 move upward, and then the sponge strip 77 fits against the lens surface of the imaging body 4. Then, the trachea 7 continues to move to the right, and then the sponge strip 77 moves to the right along the lens surface of the imaging body 4, realizing mechanical cleaning of the lens surface of the imaging body 4, effectively reducing the probability of damage to the lens surface of the imaging body 4 due to operation factors such as hidden manual cleaning, effectively reducing the probability of phenomena such as light spots during chlorophyll fluorescence imaging, effectively reducing the probability of errors in the chlorophyll fluorescence imaging of seedlings, and effectively ensuring the effect and quality of seedling phenotype detection.

[0027] When the cylinder 6 and the trachea 7 move to the extreme right position, at this time the seedling is directly below the opening 63. Then the air pump 12 is started, so that the gas below the opening 63 enters the air pump 12 along the straw 71 and the trachea 7. Thus, an upward air current is formed below the opening 63. And a spiral plate 62 is provided in the cylinder 6, so that a spiral channel is formed in the cylinder 6. At this time, the gas extracted by the air pump 12 is delivered to the spiral channel through the connecting pipe 61 and the conical cylinder, and the gas in the cylinder 6 forms a spiral air current. At the same time, the spiral air current overflows outward through the opening 63, and the overflowing spiral air current and the rising air current will generate a vortex air current on the seedling, realizing the removal of the water droplets remaining on the seedling due to the sprayed atomized warm water by using the vortex air current, effectively reducing the probability of damage to the seedling due to a unidirectional air current, effectively reducing the probability of interference with subsequent chlorophyll fluorescence imaging due to the water droplets remaining on the seedling caused by the sprayed atomized warm water, and effectively ensuring the effect and quality of seedling phenotype detection.

[0028] Then the air pump 12 is stopped, and then the first electric push rod 5, the first telescopic rod 51, and the two fixing plates 64 are used to move the cylinder 6, the trachea 7 and other components back to their original positions to the right. Then, the motor 22 and the lead screw 21 are used to move the rectangular cover 8 and other components down to a predetermined position. Then, the imaging body 4 is used to display the spatial distribution and dynamic changes of chlorophyll fluorescence on the plant surface in the form of an image, reflecting the photosynthetic physiological status of the plant, and then completing the seedling phenotype detection operation.

[0029] Example 3: The second electric push rod 9 drives the slider 93 to interlock with the movable plate 92, causing the rubber net 91 and the main pipe 96 and other components to rotate. At the same time, the water pump 31 is used to transport warm water through the main pipe 96 to the multiple second atomizing nozzles 962 distributed thereon, spraying atomized water flow on the seedlings positioned on the base 1, thereby softening the shells remaining on the seedlings. Then, with the help of the magnetic attraction of the magnetic strip 98 and the electromagnet 99, combined with the elastic support of the four T-shaped rods 94 and the third elastic member 95, the rubber net 91 is driven to move in an up and down cycle, and the seedling shells are removed in a flexible flapping manner. However, during the rotation of the main pipe 96, the hose connected to the water pump 31 will be continuously pulled, increasing the probability of it becoming tangled and chaotic within the rectangular cover 8, thereby causing obstruction and interference to subsequent imaging operations.

[0030] In order to solve the above problems, Figure 11 and Figure 12 As shown, the metal tube 34 passing through the movable block 3 is slidably connected to the upper end of the movable block 3, and the upper end of the metal tube 34 is connected to the water pump 31, and the lower end of the metal tube 34 is connected to the rear end of the main pipe 96 through a hose. The metal tube 34 is used to provide a mounting carrier for the circular plate 32, and the circular plate 32 located above the movable block 3 is set on the outer end of the metal tube 34. The first elastic member 33 is restricted and installed through the circular plate 32, and the first elastic member 33 located on the outside of the metal tube 34 is set between the circular plate 32 and the movable block 3. The circular plate 32 and the metal tube 34 are returned to their original positions through the first elastic member 33. The first elastic member 33 can be a spring.

[0031] When in use, the flower pot with the seedlings planted is first positioned and placed on the base 1 using the recessed hole 11. Then, the imaging body 4, the trapezoidal cover 81 and the rectangular cover 8 are moved downward to a predetermined position using the motor 22, the screw rod 21 and the movable block 3. Then, the movable plate 92, the main pipe 96, the mounting frame 97 and other components are rotated to a horizontal state using the second electric push rod 9 and the slider 93. At the same time, the water pump 31, the main pipe 96 and the plurality of second atomizing nozzles 962 are used to atomize warm water and spray it onto the seedlings. The atomized warm water is then used to soften the shell remaining on the seedlings and reduce the adhesion between the shell and the leaves of the seedlings. Meanwhile, the main pipe 96 and multiple first atomizing nozzles 961 are used to spray atomized warm water onto the lens surface of the imaging body 4. After the movable plate 92 rotates to a horizontal state, the circuit of the electromagnet 99 is connected, so that a repulsive force is generated between the electromagnet 99 and the magnetic strip 98. Under the action of the repulsive force and with the assistance of four T-shaped rods 94, the magnetic strip 98, the mounting frame 97 and the rubber net 91 move downward, and then the rubber net 91 comes into contact with the top of the seedling. Under the elastic force of the third elastic member 95, the magnetic strip 98, the mounting frame 97 and the rubber net 91 move upward and return to their original positions, and then the mounting frame 97 and the rubber net 91 move up and down, so as to flexibly pat the top of the seedling with the rubber net 91, and then the upper shell of the seedling falls off.

[0032] After the upper shell of the seedling leaf is removed, the second electric push rod 9 is used to turn the movable plate 92 and other components back to their original positions, and then the motor 22 and the lead screw 21 are used to adjust the height of the rectangular cover 8 and other components. Then the first electric push rod 5 is started, so that with the assistance of the first telescopic rod 51, the two fixing plates 64 move to the right, and then the cylinder 6 and the air pipe 7 move to the right, and the mounting seat 72, the second telescopic rod 73, the trapezoidal block 75, the mounting plate 76 and the sponge strip 77 will move to the right. Under the elastic force of the second elastic member 74, the sponge strip 77 moves up and fits with the lens surface of the imaging body 4, and then the sponge strip 77 moves to the right along the lens surface of the imaging body 4, so as to mechanically clean the lens surface of the imaging body 4; When the cylinder 6 and the air pipe 7 move to the extreme right position, the air pump 12, multiple suction pipes 71, the air pipe 7, the cylinder 6, the spiral plate 62 and the opening 63 are used to generate a vortex air flow on the seedling, so as to remove the water droplets remaining on the seedling due to the sprayed atomized warm water by the vortex air flow. Then, the first electric push rod 5, the first telescopic rod 51 and the two fixing plates 64 are used to move the cylinder 6 and the air pipe 7 and other components back to the right. Then, the motor 22 and the lead screw 21 are used to move the rectangular cover 8 and other components down to a predetermined position. Then, the imaging body 4 is used to display the spatial distribution and dynamic changes of chlorophyll fluorescence on the plant surface in the form of images, reflecting the photosynthetic physiological conditions of the plant, and then completing the seedling phenotype detection operation.

[0033] During the downward rotation of the movable plate 92, the hose will be pulled, and then the metal pipe 34 will move downward, so that the circular plate 32 will move downward, and then the first elastic member 33 will be compressed, and the first elastic member 33 will generate an elastic force. When the movable plate 92 rotates upward and returns to its original position, under the elastic force of the first elastic member 33, the circular plate 32 and the metal pipe 34 move upward and return to their original positions, and then the hose is pulled upward and returns to its original state, realizing the arrangement of the hose, effectively reducing the probability of blocking the imaging operation due to factors such as the hose being in a mess, and effectively ensuring the effect and quality of the seedling phenotype detection.

[0034] Although this specification is described in terms of embodiments, not every embodiment contains only one independent technical solution. This narrative style of the specification is only for clarity. Those skilled in the art should regard the specification as a whole, and the technical solutions in each embodiment can also be appropriately combined to form other embodiments that can be understood by those skilled in the art.

Claims

1. A chlorophyll fluorescence imager for seedling phenotype detection, characterized in that, Comprising: A base (1), with a lifting member installed at the upper end of the base (1); An imaging main body (4), connected to the movable part of the lifting member; A trapezoidal cover (81), connected to the lower end of the imaging main body (4), the imaging main body (4) extends into the trapezoidal cover (81), and the trapezoidal cover (81) is arranged with a narrow upper part and a wide lower part; A rectangular cover (8), communicated and arranged with the lower end of the trapezoidal cover (81); A movable plate (92), movably connected to the inner right wall of the rectangular cover (8), the movable plate (92) is arranged in a left-high and right-low inclination, and the movable plate (92) extends into the trapezoidal cover (81); A first driving member, connected to the right end of the trapezoidal cover (81), the movable part of the first driving member passes through the trapezoidal cover (81) and is movably connected to the right end of the movable plate (92); A second driving member, connected to the left end of the movable plate (92); A rubber net (91), connected to the left end of the second driving member; A main pipe (96), connected to the left end of the movable plate (92), the main pipe (96) is located above the second driving member and on the right side of the imaging main body (4); A first atomizing nozzle (961), there are multiple first atomizing nozzles (961), multiple first atomizing nozzles (961) are equidistantly communicated and arranged at the upper end of the main pipe (96), and a first control valve is assembled on the first atomizing nozzle (961); A second atomizing nozzle (962), there are multiple second atomizing nozzles (962), multiple second atomizing nozzles (962) are equidistantly communicated and installed at the lower left end of the main pipe (96), and a second control valve is assembled on the second atomizing nozzle (962); An air-drying member, connected to the left end of the rectangular cover (8), the air-drying member extends into the rectangular cover (8) and is located on the left side of the imaging main body (4).

2. The chlorophyll fluorescence imager for seedling phenotype detection according to claim 1, wherein: The air-drying member includes a cylinder (6), the cylinder (6) is slidably connected to the left end of the rectangular cover (8) and extends into the rectangular cover (8), a conical cylinder is communicated and arranged at the left end of the cylinder (6), and the conical cylinder is arranged with a narrow left part and a wide right part, a connecting pipe (61) is communicated and installed at the left end of the conical cylinder, the other end of the connecting pipe (61) is communicated and arranged with a first extraction device, the first extraction device is arranged at the upper end of the base (1) and is located at the rear end of the lifting member, a spiral plate (62) is installed in the cylinder (6), the lower end surface of the cylinder (6) is recessed upward to form an opening (63), and the opening (63) extends to the inner wall of the cylinder (6); Fixing plates (64) are installed at both the front and rear ends of the cylinder (6), and the fixing plates (64) are located in the rectangular cover (8), a telescopic device is arranged at the left end of the rectangular cover (8) and is located at the rear side of the cylinder (6), a first telescopic rod (51) is installed at the left end of the rectangular cover (8) and is located at the front side of the cylinder (6), the movable parts of the telescopic device and the first telescopic rod (51) both pass through the rectangular cover (8) and are respectively connected to the left ends of the two fixing plates (64).

3. The chlorophyll fluorescence imager for seedling phenotype detection according to claim 2, characterized in that: An air pipe (7) is installed at the upper end of the cylinder (6), and the right end of the air pipe (7) extends into the rectangular cover (8). A plurality of suction pipes (71) are equidistantly communicated and arranged at the lower end of the air pipe (7), and the lower ends of the suction pipes (71) penetrate through the cylinder (6) and the opening (63). The left end of the air pipe (7) is communicated with the inlet part of the first extraction device. A cleaning component is installed at the upper end of the air pipe (7), and the upper end of the cleaning component extends into the trapezoidal cover (81) and fits with the inner wall of the trapezoidal cover (81).

4. The chlorophyll fluorescence imager for seedling phenotype detection according to claim 3, wherein: The cleaning component includes a mounting seat (72). The mounting seat (72) is arranged at the upper end of the air pipe (7). A trapezoidal block (75) is arranged directly above the mounting seat (72), and the trapezoidal block (75) is arranged with a narrow upper part and a wide lower part. The inclined surface of the trapezoidal block (75) fits with the inner left wall of the trapezoidal cover (81). Two second elastic members (74) are symmetrically installed between the mounting seat (72) and the trapezoidal block (75). Two second telescopic rods (73) are symmetrically arranged between the mounting seat (72) and the trapezoidal block (75), and the two second telescopic rods (73) are respectively located inside the two second elastic members (74). An installation plate (76) is arranged at the right end of the trapezoidal block (75). A cleaning strip is installed at the upper end of the installation plate (76), and the upper end of the cleaning strip fits with the inner wall of the trapezoidal cover (81) in a squeezing manner.

5. The chlorophyll fluorescence imager for seedling phenotype detection according to claim 1, wherein: The second driving member includes a mounting frame (97). A rubber net (91) is arranged inside the mounting frame (97), and the mounting frame (97) is located on the left side of the movable plate (92) and below the main pipe (96). T-shaped rods (94) are installed at the four corner positions at the right end of the mounting frame (97). The rod parts of the four T-shaped rods (94) penetrate through the movable plate (92), and the T-shaped rods (94) are slidably connected with the movable plate (92). Third elastic members (95) are arranged at the four corner positions at the right end of the movable plate (92). The other ends of the four third elastic members (95) are respectively connected to the left ends of the plate parts of the four T-shaped rods (94), and the four third elastic members (95) are respectively located outside the rod parts of the four T-shaped rods (94). Two magnetic strips (98) are symmetrically arranged at the right end of the mounting frame (97), and the two magnetic strips (98) are located above and below the rubber net (91). Two electromagnets (99) are symmetrically installed at the left end of the movable plate (92), and the two electromagnets (99) are respectively located directly to the right of the two magnetic strips (98). The electromagnets (99) and the magnetic strips (98) are arranged to repel each other, and the electromagnets (99) and the magnetic strips (98) are both located outside the T-shaped rods (94).

6. The chlorophyll fluorescence imager for seedling phenotype detection according to claim 5, wherein: The first driving member includes a lifting device. The lifting device is installed at the upper right end of the trapezoidal cover (81). A slider (93) is slidably connected to the right end of the movable plate (92), and the slider (93) is located outside the T-shaped rods (94). The movable part of the lifting device passes through the trapezoidal cover (81) and is movably connected to the slider (93).

7. The chlorophyll fluorescence imager for seedling phenotype detection according to claim 5, wherein: The lifting member includes a mounting box (2), the mounting box (2) is arranged on the upper end of the base (1), a lead screw (21) is rotatably connected inside the mounting box (2), a driving device is installed at the upper end of the mounting box (2), and the output shaft of the driving device is connected to the lead screw (21). The outer end of the lead screw (21) is connected to a movable block (3) through a ball screw pair. The movable block (3) is slidably connected inside the mounting box (2). The movable block (3) extends out of the front side of the mounting box (2) and is connected to the imaging body (4). Two guide rods (23) are symmetrically installed inside the mounting box (2), and the two guide rods (23) are located on the left and right sides of the lead screw (21). Both of the two guide rods (23) penetrate through the movable block (3), and the movable block (3) is slidably connected to the guide rods (23).

8. The chlorophyll fluorescence imager for seedling phenotype detection according to claim 7, characterized in that: A concave hole (11) is formed by downward depression on the upper end surface of the base (1), and the concave hole (11) is located directly below the rectangular cover (8). A second extraction device is arranged at the upper end of the movable block (3), and the second extraction device is located on the front side of the mounting box (2). The rear end of the main pipe (96) is communicated with the second extraction device through a hose.

Images