Temperature-control moisturizing high-flux polyacrylamide gel preparation box
Through the gel preparation box with constant temperature control and moisturizing functions, the problem of uncontrollable temperature and humidity in traditional gel preparation is solved, long-term preservation and efficient preparation of gels are achieved, and the preparation efficiency and quality stability are improved.
Patent Information
- Application Number
- CN202510789046.4
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-06-13
- Publication Date
- 2025-08-01
AI Technical Summary
During the preparation process of traditional polyacrylamide gels, the temperature and humidity are uncontrollable, resulting in uneven gel pore size, risk of explosive aggregates, and difficulty in batch operation and low efficiency.
A gel preparation box with integrated constant temperature control and moisturizing functions is designed, using a sealed and moisturizing system, a temperature control system and a high-throughput preparation structure. The precise control of humidity and temperature is achieved through silicone sealing rings, porous hydrophilic material layer and temperature control module, and the inclined angle design is used to improve the preparation efficiency.
The gel is long-term preservation and efficient preparation, the gel quality is stable, the storage period is extended to 180 days, the preparation efficiency is increased by 60%, and the unevenness of the bubbles and separation glue interface is reduced.
Smart Images
Figure FT_1 
Figure FT_2 
Figure FT_3
Abstract
Description
Technical Field
[0001] The present invention relates to the technical field of biological experimental equipment, and particularly relates to a preparation device for polyacrylamide gel electrophoresis integrating functions of constant temperature control, humidity maintenance, and multi-channel synchronous preparation. Background Art
[0002] The traditional method for preparing polyacrylamide gel uses the glass plate sandwich method, which has the following defects: Temperature sensitivity: Fluctuations in ambient temperature result in uncontrollable gel solidification rates. Low temperatures (<15°C) delay polymerization, while high temperatures (>30°C) accelerate polymerization, affecting the uniformity of gel pore size. Additionally, the polymerization process itself is an exothermic reaction, and the combined effects of heat accumulation and high-temperature acceleration lead to explosive polymerization and loss of control during the gel formation process.
[0003] Humidity uncontrollability: Gels are prone to dehydration when exposed to air, forming gaps between the gel and the gel plate, causing sample leakage during loading or electrophoresis distortion. Batch production bottleneck: The traditional configuration process has a high probability of glue leakage. The glue preparation device requires a large amount of space, the vertical pouring speed of the glue is difficult to control, the bubble rate is high, and the interface between the concentrated gel and the separating gel during one-step pouring is easily disturbed. Summary of the Invention
[0004] Object of the Invention To provide a gel preparation box integrating functions of constant temperature control, saturated humidity maintenance, and multi-channel synchronous preparation, and to solve the technical problems of low gel preparation efficiency, short storage period, and unstable quality. Technical Solution
[0005] The preparation box includes: Sealed humidity control system ( Figure 1-2 ): A cuboid box body (100) and a detachable box cover (101) are locked through a buckle structure (102); A silica gel sealing ring (103) is embedded at the edge of the box cover to form an airtight space; A porous hydrophilic material layer (104, such as polyurethane sponge) is provided on the inner wall of the box body and is suspended above the water storage layer through a support rod (105); water is absorbed and volatilized through capillary action to maintain a humidity of ≥95%RH.
[0006] Temperature regulation system ( Figure 2 ): The heating and temperature control base 112 is internally provided with a heating wire, a temperature sensor, a time delay relay, and a temperature control module; The temperature control module receives the sensor signal and adjusts the water temperature (range: 30°C, accuracy ±0.5°C) through the PID algorithm; The water storage layer is provided with a water level indicator line (106), so that the heat generated by the gel polymerization can be quickly conducted to the water in contact with the rubber plate, and the excess heat can be carried away by the convection of the water to prevent heat accumulation.
[0007] High-throughput preparation structure ( Figure 3 ): The detachable grid-shaped partition plate (201) divides the box body into 5-10 independent units; The rubber plate (202) abuts against the partition plate at an inclination angle of 50°-80° to form a self-locking structure. Beneficial effects
[0008] Improved storage period: In a saturated humidity environment, the storage period of the prefabricated gel is extended from 8 hours at room temperature with an open mouth to 180 days; Optimized gel quality: A constant temperature environment (30°C) is achieved in a water bath manner, avoiding explosive polymerization or non-gelling; The inclined angle design reduces the occurrence of bubbles formed by the vertical impact of the liquid and the uneven interface of the separating gel.
[0009] Doubled preparation efficiency: 5-10 gels are prepared synchronously at a time, reducing the time cost by 60%, The design of the partition plate saves the space for preparing the glue. Description of the drawings
[0010] Figure 1 : Schematic diagram of the box structure (101 box cover, 102 buckle, 103 sealing ring, 111 box body, 112, heating and temperature control base) Figure 2 : Structure diagram of the partition plate and the water absorption layer (104 water absorption layer, 105 water absorption layer support rod, 106 water level indicator line, 201 partition plate, 202 rubber plate) Figure 3 : Multi-connected preparation state diagram Detailed implementation manners
[0011] Example 1: ① Inject deionized water into the water storage layer (105) to the water level line (106); ② Set the water temperature to 30°C and the time setting. The temperature control module (109) starts heating to the steady state and automatically shuts down after reaching the time setting; ③ Lean one side of 5 groups of rubber plates (202) against the partition plate, and the bottom end abuts against the bottom of the previous partition plate to automatically form a fixed inclination angle; ④ After pouring the glue, fasten the box cover, and the buckle (102) locks the sealing ring (103); ⑤ If a neutral glue-making solution is used, the prepared gel can be placed at room temperature for 180 days, The glue can be taken out and used at any time during this period. After closing the box lid, due to the existence of the porous hydrophilic polymer material layer, the water vapor saturation will be quickly restored.
Claims
1. A temperature-controlled and humidity-preserving high-throughput gel preparation box, characterized in that, Comprising: A sealed box body (111) and a box cover (101), forming an airtight chamber through a sealing ring (103); A water storage layer and a porous hydrophilic material layer (104) are arranged inside the box body. When storing the gel, the liquid levels of the porous hydrophilic material layer and the water storage layer are in contact; During the process of preparing the gel, at least 30% of the surface of the gel plate is in contact with the liquid in the water storage layer; The liquid in the water storage layer contains at least more than 50% of water.
2. The preparation box according to claim 1, wherein: The porous hydrophilic material layer (104) is a polyurethane sponge, or a polyvinyl alcohol sponge, or a highly absorbent non-woven fabric.
3. The preparation box according to claim 1, characterized in that: The pure water or aqueous solution in the water storage layer can be heated to a specified temperature by an external heater and then injected, or can be directly heated and temperature-controlled by a temperature control element inside or at the bottom of the water storage layer.
4. The preparation box according to claim 1, wherein: The connection part of the sealed box body (111) and the box cover (101) has a buckle structure (102) to increase the acting force between the two.
5. The preparation box according to claim 1, characterized in that: Inside the sealed box body (111), 2-12 gels can be prepared simultaneously.
6. The preparation box according to claim 1, characterized in that: A support structure (201) is distributed inside the sealed box body, so that the gel plate (202) maintains an inclination angle of 50° to 80° with the horizontal plane during the gel preparation process.
7. The preparation box according to claim 1, characterized in that: The support structure (201) is distributed inside the sealed box body, and the spacing can be adjusted, so as to adjust the distribution and quantity of the gel plates (202) inside the sealed box body.