Qualitative detection method for floral components of living plant, quantitative method for release amount of floral components of living plant and collection device of floral components of living plant

Abstract

The invention belongs to the technical field of plant aroma component detection, and particularly relates to a qualitative detection method for aroma components of living plants, a quantitative method for release amount of the aroma components and a collection device of the aroma components. The qualitative method comprises the following steps: trapping and adsorbing plant flower fragrance in a natural growth state; sequentially carrying out thermal desorption and gas chromatography-mass spectrometry detection on the captured and adsorbed plant flower fragrance to obtain a total ion chromatogram of the plant flower fragrance; and carrying out spectrum library retrieval analysis on the total ion chromatogram of the plant flower fragrance, and qualitatively determining the components of the plant flower fragrance. According to the present invention, the plant flower fragrance in the natural growth state is captured, the plant tissue is not damaged, and the impurity interference does not exist, such that the influence of the stress reaction generated by the fresh flower in vitro on the flower fragrance component can be avoided so as to accurately perform the qualitative and quantitative analysis on the flower fragrance component.

Description

Technical Field

[0001] The present invention belongs to the technical field of plant aroma component detection, and in particular relates to a qualitative detection method for floral aroma components of living plants, a quantitative method for determining their release amounts, and a collection device thereof. Background Art

[0002] Floral fragrance, a secondary metabolite released by plant flowers, exhibits remarkable diversity and is a key quality of ornamental plants. Taking peony fragrance as an example, over 90 compounds have been identified to date. Huang Xue et al., using headspace solid-phase microextraction and gas chromatography-mass spectrometry, detected 34 aroma components in the peony cultivar "Yang Fei Chu Yu" and 36 in "Da Fu Gui." Hou Yixuan et al. analyzed the volatile components of the petals of 19 intergroup hybrids in the genus Paeonia, detecting a total of 84 volatile components. Song Chaowei et al. identified the aroma components of different floral organs of two aromatic peony cultivars, "Qiao Ling" and "Yang Fei Chu Yu."

[0003] The aroma composition of plant flowers depends primarily on the variety, but is also influenced by the collection method. Currently, aroma analysis primarily relies on headspace analysis, solid-phase microextraction, and gas chromatography-mass spectrometry. However, these methods can only detect aroma components from isolated flowers. In vitro sampling of flowers inevitably triggers physiological and biochemical stress responses, which may interfere with the synthesis and release of aroma components. Summary of the Invention

[0004] The purpose of the present invention is to provide a qualitative detection method for floral fragrance components of living plants, a quantitative method for determining their release amounts, and a collection device thereof. The qualitative detection method provided by the present invention can detect floral fragrance components in living plants, avoiding the influence of stress reactions generated by fresh flowers in vitro on the floral fragrance components.

[0005] In order to achieve the above object, the present invention provides the following technical solutions:

[0006] The present invention provides a method for qualitatively detecting the floral fragrance components of living plants, comprising the following steps:

[0007] Capture and absorb the fragrance of plants and flowers in their natural growth state;

[0008] The captured and adsorbed plant fragrance is subjected to thermal desorption and gas chromatography-mass spectrometry detection in sequence to obtain a total ion current diagram of the plant fragrance;

[0009] The total ion current spectrum of plant floral fragrance was searched and analyzed by library to qualitatively identify the components of plant floral fragrance.

[0010] Preferably, the capture and adsorption step comprises:

[0011] (1) Put multiple plant flowers with the same variety and flowering period into a sampling bag. Use an air sampler to evacuate the air in the sampling bag, and then fill it with pre-treated air.

[0012] The pre-treatment includes activated carbon filtration and discolored silica gel filtration carried out in sequence.

[0013] (2) After inflation, connect a thermal desorption tube to the intake end of the air sampler, and capture and adsorb the floral fragrance components through closed-loop circulation.

[0014] Preferably, when capturing and adsorbing the floral fragrance components, the gas flow rate of the air sampler is 0.2 - 0.5 L / min, and the capture and adsorption time is 0.5 - 1 h.

[0015] Preferably, the pre-treated air accounts for 80% of the volume of the sampling bag.

[0016] Preferably, the packing material in the thermal desorption tube is Tenax GR 60 / 80; the sampling bag is a PVF sampling bag.

[0017] Preferably, the temperature of the thermal desorption is 230 - 280 °C, and the time of the thermal desorption is 5 - 20 min.

[0018] Preferably, the conditions for gas chromatography - mass spectrometry detection include:

[0019] The gas chromatography column is a DB-5MS gas capillary chromatography column;

[0020] The temperature programming of the gas chromatography column is: the initial temperature is 40 °C and is maintained for 2 min, then it is heated at a heating rate of 6 °C / min to 180 °C and is maintained at 180 °C for 0 - 5 min;

[0021] The mass spectrometry ionization mode is the EI source; the ion source temperature is 230 °C; the scanning mass range is 29 - 600 amu.

[0022] Preferably, the plant flowers include peonies.

[0023] The present invention also provides a method for quantitatively determining the release amount of floral fragrance components of living plants, including the following steps:

[0024] Qualitatively detect the floral fragrance components of living plants according to the qualitative detection method described in the above technical solution;

[0025] According to the peak areas of the floral fragrance components of living plants detected qualitatively and the predetermined standard curves of each floral fragrance component, obtain the contents of each floral fragrance component by the external standard method;

[0026] According to formula I, calculate the release amounts of each floral fragrance component;

[0027]

[0028] In Formula I: v i is the release amount of the floral fragrance component, ng / g·h;

[0029] x i is the content of each floral fragrance component in the flower to be measured obtained from a predetermined standard curve, μg;

[0030] M is the weight of the flower of the plant to be measured, g; t is the sampling time, h.

[0031] The present invention provides a collection device for the floral fragrance components of a living plant. The collection device includes a sampling bag; a thermal desorption tube connected to the outlet of the sampling bag; an air sampler connected to the outlet of the thermal desorption tube; a first pretreatment unit communicated with the outlet of the air sampler; a second pretreatment unit communicated with the outlet of the first pretreatment unit; the outlet of the second unit is communicated with the inlet of the sampling bag;

[0032] The outlet of the thermal desorption tube is communicated with the inlet of the air sampler;

[0033] The inlet of the first pretreatment unit is communicated with the outlet of the air sampler;

[0034] The inlet of the second pretreatment unit is communicated with the outlet of the first pretreatment unit;

[0035] The first pretreatment unit includes a first drying tower containing activated carbon;

[0036] The second pretreatment unit includes a second drying tower containing discolored silica gel.

[0037] The present invention captures the floral fragrance of plants in a natural growth state, does not damage the plant tissues and has no impurity interference. Therefore, it can avoid the influence of the stress response generated by the separation of fresh flowers from the plant on the floral fragrance components, making the qualitative analysis of the floral fragrance components more accurate.

[0038] Furthermore, the floral fragrance collection method of the present invention has the advantages of simple steps and easy operation.

[0039] Furthermore, the present invention calculates the absolute release amount of each floral fragrance component by the external standard method, and the result is more accurate and has higher credibility compared with the quantification of the relative content of the floral fragrance components in the prior art. BRIEF DESCRIPTION OF THE DRAWINGS

[0040] In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the drawings required to be used in the embodiments. Obviously, the drawings described below are only some embodiments of the present invention. For those of ordinary skill in the art, other drawings can be obtained based on these drawings without creative efforts.

[0041] Figure 1 Schematic diagram of the collection device for floral fragrance components of living plants;

[0042] Figure 2 Total ion chromatogram of floral fragrance components of Paeonia lactiflora 'Chunxiao';

[0043] Figure 3 Total ion chromatogram of floral fragrance components of Paeonia lactiflora 'Qingwen';

[0044] Figure 4 Total ion chromatogram of floral fragrance components of Paeonia lactiflora 'Taohuafeixue'. Specific implementation mode

[0045] The present invention provides a qualitative detection method for floral fragrance components of living plants, comprising the following steps:

[0046] Collect and adsorb the floral fragrance of plants in the natural growth state;

[0047] Thermally desorb and perform gas chromatography - mass spectrometry detection on the collected and adsorbed floral fragrance of plants in sequence to obtain the total ion chromatogram of the floral fragrance of plants;

[0048] Perform library search and analysis on the total ion chromatogram of the floral fragrance of plants to qualitatively analyze the floral fragrance components.

[0049] The present invention collects and adsorbs the floral fragrance of plants in the natural growth state. As an implementation mode of the present invention, the plant is preferably Paeonia lactiflora.

[0050] As an implementation mode of the present invention, the step of collecting and adsorbing the floral fragrance of plants in the natural growth state can be:

[0051] (1) Put multiple plant flowers with the same variety and flowering period into a sampling bag, use an air sampler to evacuate the air in the sampling bag, and then fill it with pretreated air;

[0052] The pretreatment includes activated carbon filtration and silica gel filtration with color change in sequence;

[0053] (2) After the inflation is completed, connect a thermal desorption tube to the intake end of the air sampler, and collect and adsorb the floral fragrance components by closed - loop circulation.

[0054] As an implementation mode of the present invention, the sampling bag is preferably a PVF sampling bag; the number of plant flowers ≥ 3, and in the embodiments of the present invention, specifically 5 flowers are taken as an example for illustration. As an implementation mode of the present invention, before putting into the sampling bag, it also includes cleaning the dust and honeydew on the sepals.

[0055] As an embodiment of the present invention, preferably, the sampling bag is filled with pretreated air accounting for 80% of the volume of the sampling bag. As an embodiment of the present invention, when collecting and adsorbing the floral fragrance components, the gas flow rate of the air sampling instrument can be 0.2 - 0.5 L / min, specifically 0.5 L / min, and the collection time can be 0.5 - 1 h, specifically 1 h.

[0056] As an embodiment of the present invention, the thermal desorption tube can be a CAMSCO stainless steel thermal desorption tube, and the specifications can be: 1 / 4 inch × 3.5 inches (outer diameter × length), and the packing can be Tenax GR 60 / 80.

[0057] Figure 1 It is a schematic diagram of the collection device for the floral fragrance components of living plants, as Figure 1 shown, the collection device includes a sampling bag; a thermal desorption tube connected to the outlet of the sampling bag; an air sampling instrument connected to the outlet of the thermal desorption tube; a first pretreatment unit communicated with the outlet of the air sampling instrument; a second pretreatment unit communicated with the outlet of the first pretreatment unit; the outlet of the second unit is communicated with the inlet of the sampling bag; the outlet of the thermal desorption tube is communicated with the inlet of the air sampling instrument; the inlet of the first pretreatment unit is communicated with the outlet of the air sampling instrument; the inlet of the second pretreatment unit is communicated with the outlet of the first pretreatment unit; the first pretreatment unit includes a first drying tower containing activated carbon; the second pretreatment unit includes a second drying tower containing a discolored silica gel column.

[0058] As an embodiment of the present invention, the inlet of the first pretreatment unit is arranged above the first drying tower; the outlet of the first pretreatment unit is arranged on the side of the first drying tower; the inlet of the second pretreatment unit is arranged above the second drying tower; the outlet of the second pretreatment unit is arranged on the side of the second drying tower.

[0059] The present invention thermally desorbs and then performs gas chromatography - mass spectrometry detection on the collected and adsorbed floral fragrance of plants to obtain the total ion chromatogram of the floral fragrance of plants.

[0060] As an embodiment of the present invention, the temperature of the thermal desorption can be 230 - 280 °C, specifically 260 °C, and the desorption time can be 5 - 20 min, specifically 10 min.

[0061] The conditions for the gas chromatography - mass spectrometry detection include:

[0062] The gas chromatography column is a DB-5MS gas capillary chromatography column, and its specification can be 30 m × 0.25 mm × 0.25 μm. As an embodiment of the present invention, the temperature programming of the gas chromatography column is as follows: the initial temperature is maintained at 40°C for 2 min, then it is heated to 180°C at a heating rate of 6°C / min and maintained at 180°C for 0 - 5 min; the mass spectrometry ionization mode is the EI source; the ion source temperature is 230°C; the scanning mass range is 29 - 600 amu.

[0063] As an embodiment of the present invention, the thermal desorption and gas chromatography - mass spectrometry detection are preferably carried out on a thermal desorption - gas chromatography - mass spectrometry instrument.

[0064] The present invention performs library search analysis on the total ion current chromatogram of plant floral scents to qualitatively analyze the components of plant floral scents.

[0065] As an embodiment of the present invention, it is preferred to use the NIST17 library to perform library search analysis on the total ion current chromatogram of the plant floral scents to qualitatively analyze the components of plant floral scents.

[0066] The present invention also provides a method for quantifying the release amount of the components of living plant floral scents, including the following steps:

[0067] Qualitatively detect the components of living plant floral scents according to the above - mentioned technical solution;

[0068] According to the peak areas of the components of living plant floral scents detected qualitatively and the predetermined standard curves of each floral scent component, the content of each floral scent component is obtained by the external standard method;

[0069] According to Equation I, calculate the release amount of each floral scent component;

[0070]

[0071] In Equation I: v i is the release amount of the floral scent component, ng / g·h; x i is the content of each floral scent component in the flower to be measured obtained from the predetermined standard curve, μg; M is the weight of the flower of the plant to be measured, g; t is the sampling time, h.

[0072] The predetermined standard curve is the linear relationship between the concentration of each aroma component and its peak area.

[0073] As an embodiment of the present invention, the acquisition of the predetermined standard curves of each floral scent component includes the following steps:

[0074] Prepare a series of concentration standard solutions of each aroma component respectively;

[0075] The series concentrations of each aroma component were successively subjected to trapping adsorption, thermal desorption, and gas chromatography-mass spectrometry detection to obtain the peak areas of each aroma component.

[0076] Based on the concentrations and peak areas of each aroma component, a linear relationship between the two was established to obtain the standard curve of each aroma component.

[0077] As an embodiment of the present invention, the release amount of the plant flower fragrance component is calculated according to Formula I:

[0078]

[0079] In the formula: v i is the flower fragrance release amount, ng / g·h; x i is the content of each flower fragrance component in the flower to be measured obtained from the predetermined standard curve, μg; M is the weight of the plant flower to be measured, g; t is the sampling time, h.

[0080] Flower fragrance is determined by the aroma components and their contents. The currently commonly used method for quantifying flower fragrance components is the area normalization method. This method is simple to operate, but the obtained is the relative content of the components, so the quantitative result is inaccurate and the credibility is relatively low. The present invention calculates the absolute release amount of each flower fragrance component by the external standard method, and the quantitative result is more accurate.

[0081] To further illustrate the present invention, the following describes the solution of the present invention in detail with reference to the drawings and embodiments, but they should not be construed as limiting the protection scope of the present invention. This method takes the collection and detection of the flower fragrance components and release amounts of 3 different peony varieties, "Spring Dawn", "Qingwen", and "Flying Snow of Peach Blossom", as specific examples to specifically introduce the content of the invention.

[0082] Sources of materials used in the examples:

[0083] PVF sampling bags, 5L, provided by Dalian Delin Gas Packaging Co., Ltd.;

[0084] Example 1

[0085] 1) Select the flowers at the full-bloom stage of 3 peony varieties, "Spring Dawn", "Qingwen", and "Flying Snow of Peach Blossom", and collect their flower fragrance components.

[0086] As Figure 1, after cleaning the dust and honeydew on the sepals, put 5 flowering-stage flowers of the same variety into a PVF sampling bag at the same time. First, use an air sampler to exhaust the air in the bag, and then fill it with air filtered by activated carbon and silica gel (the inflation volume is 80% of the sampling bag volume). After inflation, immediately connect a thermal desorption tube to the intake end of the air sampler, close the loop to trap the floral fragrance components, and start timing. During sampling, the gas flow rate of the air sampler is 0.5 L / min, and the sampling time is 1 h. After sampling, quickly remove the sampling tube, seal it for storage, then cut the flowers in the PVF sampling bag and weigh them.

[0087] 2) Heat the sampling tube with a thermal desorption instrument at a thermal desorption temperature of 260 °C for 10 min. After desorbing the floral fragrance components collected in the sampling tube, directly analyze and detect them using gas chromatography-mass spectrometry.

[0088] Gas chromatography-mass spectrometry working conditions: EI source, the ion source temperature is 230 °C, the chromatographic column model is DB-5MS (30 m × 0.25 mm × 0.25 μm), and the column oven temperature rise process is: hold at 40 °C for 2 min, and then increase the temperature to 180 °C at a rate of 6 °C / min.

[0089] 3) The total ion chromatograms of the floral fragrances of 3 kinds of peonies, namely "Chunxiao", "Qingwen", and "Taohuafeixue", obtained by GC / MS detection are shown in Figure 2 、 Figure 3 and Figure 4 . Use the NIST17 spectral library to retrieve each chromatographic peak in the total ion chromatogram to identify the floral fragrance components.

[0090] After analysis: "Chunxiao" contains a total of 27 kinds of floral fragrance components, including 8 alcohol compounds such as pentanol, trans-3-hexen-1-ol, hexanol, cis-3-hexen-1-ol, linalool, phenethyl alcohol, citronellol, and nerol; 7 terpene compounds such as α-pinene, β-pinene, myrcene, limonene, ocimene, caryophyllene, and thujopsene; 3 ester compounds such as butyl butyrate, citronellyl acetate, and geranyl acetate; 2 aldehyde compounds such as phenylacetaldehyde and neral; 2 hydrocarbon compounds such as p-cymene and tridecane; 3 ketone compounds such as acetophenone, verbenone, and geranyl acetone; 2 ether compounds such as anisole and cineole. Among them, citronellol, phenethyl alcohol, nerol, and caryophyllene are the main components.

[0091] "Qingwen" contains a total of 34 floral fragrance components, including: 9 alcohol compounds such as pentanol, trans-3-hexen-1-ol, hexanol, cis-3-hexen-1-ol, linalool, phenethyl alcohol, terpineol, citronellol, and nerol; 10 terpene compounds such as α-pinene, β-pinene, myrcene, α-phellandrene, α-terpinene, limonene, ocimene, cedrene, caryophyllene, and thujopsene; 2 ester compounds such as citronellyl acetate and geranyl acetate; 7 aldehyde compounds such as hexanal, benzaldehyde, octanal, phenylacetaldehyde, nonanal, decanal, and neral; 2 hydrocarbon compounds such as p-cymene and tridecane; 2 ketone compounds such as acetophenone and geranylacetone; 2 ether compounds such as anisole and cineole. Among them, phenethyl alcohol and nerol are the main components.

[0092] "Taohua Feixue" contains a total of 26 floral fragrance components, including: 8 alcohol compounds such as pentanol, trans-3-hexen-1-ol, hexanol, cis-3-hexen-1-ol, linalool, phenethyl alcohol, citronellol, and nerol; 9 terpene compounds such as α-pinene, β-pinene, myrcene, limonene, ocimene, γ-terpinene, caryophyllene, thujopsene, and Farnesene; 3 ester compounds such as butyl butyrate, citronellyl acetate, and geranyl acetate; 2 aldehyde compounds such as citronellal and neral; 2 ketone compounds such as 6-methyl-5-hepten-2-one and acetophenone; 2 ether compounds such as anisole and cineole. Among them, citronellol, nerol, phenethyl alcohol, and butyl butyrate are the main components.

[0093] 4) Prepare working solutions with concentrations of 0.1, 0.2, 0.5, 1, and 2 mg / mL for the standards of each aroma component using n-hexane. Take 1 μL of each and inject it into the thermal desorption tube, and measure it with a thermal desorption-gas chromatography mass spectrometer. Obtain the standard curve of each standard according to the relationship between the content (x) and the peak area (y).

[0094] 5) Substitute the peak areas of each component in the total ion current chromatogram of the 3 kinds of peony flower fragrances into the standard curve respectively, and use the external standard method to obtain the content of each flower fragrance component in the enrichment sampling tube.

[0095] 6) Substitute the content of each flower fragrance component in the sampling tube and the weight of different varieties of fresh flowers into the following formula to calculate the release amount of each flower fragrance component of peony flowers (Table 1, Table 2, and Table 3);

[0096]

[0097] In formula I: v i is the release amount of the flower fragrance component, ng / g·h; x i is the content of each flower fragrance component in the flower to be measured obtained from the predetermined standard curve, μg; M is the weight of the flower of the plant to be measured, g; t is the sampling time, h.

[0098] Table 1 Floral Fragrance Components and Release Amounts of Paeonia lactiflora 'Chunxiao'

[0099]

[0100]

[0101] Table 2 Floral Fragrance Components and Release Amounts of Paeonia lactiflora 'Qingwen'

[0102]

[0103]

[0104] Table 3 Floral Fragrance Components and Release Amounts of Paeonia lactiflora 'Taohuafeixue'

[0105]

[0106]

[0107] Components with high release amounts and high aroma intensities are often characteristic aroma components. The component with the highest release amount in 'Chunxiao' is citronellol, reaching 218.03 ng / g·h, followed by phenethyl alcohol with a release amount of 114.58 ng / g·h. The component with the highest release amount in 'Qingwen' is phenethyl alcohol, at 72.76 ng / g·h, followed by nerol with a release amount of 55.18 ng / g·h. The release amount of citronellol in 'Taohuafeixue' is very high, at 472.35 ng / g·h, followed by nerol (126.58 ng / g·h). Citronellol, phenethyl alcohol, and nerol are all characteristic aroma components of roses, so these three Paeonia lactiflora aroma types all belong to the rose fragrance type.

[0108] There are many Paeonia lactiflora varieties, and there are obvious differences in floral fragrance components and release amounts among different varieties. This method can be used for testing the floral fragrance components of other Paeonia lactiflora varieties to obtain the true floral fragrance components and accurate release amounts of more Paeonia lactiflora varieties, providing a basis for studying the interaction relationships among different aroma components and the correlations between their release amounts and the floral fragrance intensity and fragrance type of Paeonia lactiflora.

[0109] Although the above embodiments have described the present invention in detail, they are only a part of the embodiments of the present invention, not all embodiments. Other embodiments can also be obtained based on these embodiments without creative efforts, and these embodiments all fall within the protection scope of the present invention.

Claims

1. A qualitative detection method for the floral fragrance components of living plants, characterized in that, It includes the following steps: Capture and adsorb the floral fragrance of plants in their natural growth state; Thermally desorb and perform gas chromatography - mass spectrometry detection on the captured and adsorbed floral fragrance of plants in sequence to obtain the total ion chromatogram of the floral fragrance of plants; Conduct library search and analysis on the total ion chromatogram of the floral fragrance of plants to qualitatively analyze the components of the floral fragrance of plants.

2. The qualitative detection method according to claim 1, characterized in that, The step of capture and adsorption includes: (1) Put multiple plant flowers with the same variety and flowering period into a sampling bag, use an air sampler to exhaust the air in the sampling bag, and then fill it with pre - treated air; The pre - treatment includes activated carbon filtration and silica gel filtration with color change in sequence; (2) After inflation, connect a thermal desorption tube to the inlet end of the air sampler, and capture and adsorb the floral fragrance components through closed - loop circulation.

3. The qualitative detection method according to claim 2, wherein When capturing and adsorbing the floral fragrance components, the gas flow rate of the air sampler is 0.2 - 0.5 L / min, and the capture and adsorption time is 0.5 - 1 h.

4. The qualitative detection method according to claim 2, wherein The pre - treated air accounts for 80% of the volume of the sampling bag.

5. The qualitative detection method according to claim 2, wherein The filler in the thermal desorption tube is Tenax GR60 / 80; the sampling bag is a PVF sampling bag.

6. The qualitative detection method according to claim 1, characterized in that, The temperature of the thermal desorption is 230 - 280 °C, and the time of the thermal desorption is 5 - 20 min.

7. The qualitative detection method according to claim 1, characterized in that, The conditions for the gas chromatography - mass spectrometry detection include: The gas chromatography column is a DB - 5MS gas capillary chromatography column; The temperature - rising program of the gas chromatography column is: the initial temperature is 40 °C and is maintained for 2 min, then it is heated at a rate of 6 °C / min to 180 °C and is maintained at 180 °C for 0 - 5 min; The mass spectrometry ionization mode is EI source; the ion source temperature is 230 °C; the scanning mass range is 29 - 600 amu.

8. The qualitative detection method according to claim 1, wherein The plant flowers include peonies.

9. A method for quantifying the release amount of floral fragrance components of living plants, which includes the following steps: Qualitatively detect the floral fragrance components of living plants according to the qualitative detection method described in any one of claims 1 - 8; According to the peak areas of the qualitatively detected floral fragrance components of living plants and the predetermined standard curves of each floral fragrance component, use the external standard method to obtain the content of each floral fragrance component; Calculate the release amount of each floral fragrance component according to formula I; In Formula I: v i is the release amount of the floral fragrance component, ng / g·h; x i is the content of each floral fragrance component in the flower to be measured obtained from a predetermined standard curve, μg; M is the weight of the plant flowers to be measured, g; t is the sampling time, h.

10. An acquisition device for the floral fragrance components of a living plant, characterized in that, The collection device includes a sampling bag; a thermal desorption tube connected to the outlet of the sampling bag; An air sampler connected to the outlet of the thermal desorption tube; A first pre - treatment unit communicated with the outlet of the air sampler; A second pre - treatment unit communicated with the outlet of the first pre - treatment unit; the outlet of the second unit is communicated with the inlet of the sampling bag; The outlet of the thermal desorption tube is communicated with the inlet of the air sampler; The inlet of the first pre - treatment unit is communicated with the outlet of the air sampler; The inlet of the second pre - treatment unit is communicated with the outlet of the first pre - treatment unit; The first pre - treatment unit includes a first drying tower containing activated carbon; The second pre - treatment unit includes a second drying tower containing silica gel with color change.

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