Application of body resistance strengthening and detoxification formula in preparation of porcine epidemic diarrhea virus inhibitor

Abstract

The invention relates to a formula for strengthening body resistance and detoxifying and application of at least one of kaempferol-7-O-glucoside, acradine, kaempferol and octyl gallate in preparation of drugs for resisting porcine epidemic diarrhea virus infection. In view of the strong inhibition effect of the body resistance strengthening and detoxification prescription and the compound on PEDV virus, the body resistance strengthening and detoxification prescription and the compound are likely to become specific medicines for treating swine fluid diarrhea. The inventor believes that as a potential medicine for treating PEDV infection, the body resistance strengthening and detoxification prescription and the compounds have important medicinal value, and are promising candidate medicines for treating porcine epidemic diarrhea virus infectious diseases.

Description

Technical Field

[0001] The invention belongs to the technical field of medicine, and particularly relates to an application of a Fuzheng Jiedu recipe in preparing a drug for resisting porcine epidemic diarrhea virus infection. Background Art

[0002] Porcine epidemic diarrhea is an acute intestinal infectious disease of pigs caused by porcine epidemic diarrhea virus (PEDV), with diarrhea, vomiting, and dehydration as typical symptoms. Pigs of all ages are susceptible to infection, but piglets under two weeks of age are most seriously affected. PEDV is an enveloped, single-stranded, positive-sense RNA virus with a genome size of approximately 28kb. It encodes four structural proteins and four non-structural proteins. The structural proteins include spike protein (S), envelope protein (E), membrane protein (M), and capsid protein (N), while the non-structural proteins include 1a, 1b, 3a, and 3b. PEDV is mainly transmitted through the mouth and nose. Other scholars have confirmed that contaminated feed can also transmit PEDV. In addition, studies have found that PEDV can also be transmitted in the form of aerosols. The diversity of PEDV transmission routes has brought certain difficulties to the prevention and control of epidemic diarrhea. PEDV, which enters the intestines through the mouth and nose, can proliferate within intestinal epithelial cells, causing swelling, degeneration, and disintegration. This leads to atrophy and loss of intestinal villi, decreased intestinal enzyme activity, impaired absorption, and increased intestinal osmotic pressure, causing osmotic diarrhea. Due to their poor stress tolerance and tolerance, piglets experience slow intestinal villi renewal, resulting in a much higher mortality rate than medium and larger pigs.

[0003] In pig farms where diarrhea occurs, vaccinating piglets or newborns with diarrheal vaccines containing the PEDV GII genotype has little control effect. Suckling piglets under 7 days of age are most susceptible to infection, with a mortality rate as high as 100%. Theoretically, it takes at least 7 to 10 days for piglets to develop immunity through vaccination. Currently, protecting suckling piglets through maternal antibodies (protection) in breast milk is the only effective way to control PEDV in piglets.

[0004] Drug prevention and control has been a new area of research in recent years. Currently, the main prevention and control drugs include a series of biopharmaceuticals such as monoclonal antibodies, immune sera, and egg yolk antibodies. Although these biopharmaceuticals have shown promising results, their instability and high cost have prevented them from being commercialized. Therefore, the development of effective and stable antiviral drugs is of paramount importance, and the development of safe, effective, and broad-spectrum antiviral drugs is of great significance.

[0005] Academician Liu Liang and his team developed a traditional Chinese medicine formula for strengthening the body and detoxifying the body based on the common symptoms of patients infected with the Delta strain. It consists of the following eight herbs: 10g of Radix Aconiti Lateralis Preparata, 15g of dried ginger, 20g of roasted licorice root, 10g of honeysuckle flower, 10g of thorny Chinese honeysuckle, 20g of quinquefolia, 10g of patchouli, and 5g of dried tangerine peel, totaling 100g. The granules are prepared by adding eight times the weight of water to the above formula and decocting the mixture three times for one hour each time. The decoction is filtered, the filtrates are combined, and concentrated under reduced pressure at 80°C (vacuum pressure of -0.090 to -0.1 MPa) to produce an extract (each gram of extract is equivalent to approximately 2g of the slices). Stevia (0.5% by mass) and lactose-dextrin (2:1 by mass) are then added. Granulation is then performed in a single step to produce 1000g of granules, which are then packaged separately. At present, the Fu Zheng Jie Du Fang granules have shown good therapeutic effects on 274 patients infected with the Delta variant of the new coronavirus in clinical practice, indicating that this prescription has the effect of blocking patients from turning into severe high-risk / warning groups in the treatment of new coronavirus infections, promoting the absorption of lung inflammation, and shortening the time for nucleic acid to turn negative.

[0006] However, there are currently no reports on the use of Fuzheng Jiedu prescription to treat porcine epidemic diarrhea virus infection. Summary of the Invention

[0007] The present invention provides a Fuzheng Jiedu recipe and use of at least one of the following compounds in a medicament for preventing, alleviating and / or treating diseases caused by porcine epidemic diarrhea virus (PEDV): kaempferol-7-O-glucoside, acladine, kaempferol, and octyl gallate.

[0008] According to an embodiment of the present invention, the Fuzheng Jiedu prescription is composed of the following 8 Chinese medicines in parts by mass: 10 parts of Aconite Root, 15 parts of Dried Ginger, 20 parts of Roasted Licorice Root, 10 parts of Honeysuckle, 10 parts of Sophora japonica, 20 parts of Prunus mume, 10 parts of Patchouli Fragrantis, and 5 parts of Tangerine Peel, totaling 100 parts.

[0009] According to an embodiment of the present invention, the Fuzheng Jiedu prescription was formulated by Academician Liu Liang and his team based on the general pattern of symptoms in patients infected with the Delta strain.

[0010] According to an embodiment of the present invention, the Fu Zheng Jie Du Fang is a Fu Zheng Jie Du Fang granule, and its preparation method is as follows: add 8 times the mass of water to the above prescription, decoct three times, each decoction for 1 hour, filter the decoction, combine the filtrates, and concentrate under reduced pressure at 80°C (vacuum degree is -0.090 to -0.1MPa) to form an extract (each gram of extract is equivalent to approximately 2g of medicinal slices), add stevia with a mass fraction of 0.5% and lactose-dextrin (mass ratio 2:1) in an appropriate amount, granulate in one step, make 1000g of granules, and package them.

[0011] According to an embodiment of the present invention, the Fuzheng Jiedu Fang granules and at least one of the following compounds inhibit porcine epidemic diarrhea virus (PEDV), or treat diseases caused by porcine epidemic diarrhea virus (PEDV) infection, or improve diseases caused by porcine epidemic diarrhea virus (PEDV) infection: kaempferol-7-O-glucoside, aclaidine, kaempferol, and octyl gallate.

[0012] According to an embodiment of the present invention, the inhibition of porcine epidemic diarrhea virus (PEDV) may be at the organism level or the cell level.

[0013] According to an embodiment of the present invention, the symptoms caused by porcine epidemic diarrhea virus (PEDV) infection may be diarrhea, vomiting, or dehydration.

[0014] According to an embodiment of the present invention, the Fuzheng Jiedu Recipe and at least one of the following compounds inhibit porcine epidemic diarrhea virus (PEDV) by exerting its effect after entering the host or host cell (i.e., post-endocytosis). Therefore, the Fuzheng Jiedu Recipe granules and at least one of the following compounds are suitable for preparing a product for preventing, alleviating, and / or treating diseases caused by porcine epidemic diarrhea virus (PEDV) infection: kaempferol-7-O-glucoside, acladine, kaempferol, and octyl gallate.

[0015] The present invention also provides a pharmaceutical composition for preventing, alleviating and / or treating diseases caused by porcine epidemic diarrhea virus (PEDV) infection, which comprises the above-mentioned Fuzheng Jiedu prescription and at least one of the following compounds: kaempferol-7-O-glucoside, acladine, kaempferol, and octyl gallate.

[0016] According to an embodiment of the present invention, the pharmaceutical composition further comprises a pharmaceutically acceptable excipient.

[0017] According to an embodiment of the present invention, the pharmaceutically acceptable excipient includes one, two or more of a physiologically or pharmaceutically acceptable carrier, diluent, vehicle and / or excipient.

[0018] According to an embodiment of the present invention, the pharmaceutical composition can be formulated into various pharmaceutical preparation forms suitable for administration.

[0019] According to an embodiment of the present invention, the preparation form is selected from granules, tablets, capsules, solutions, suspensions and semisolid preparations.

[0020] The present invention also provides a method for preventing, alleviating and / or treating diseases caused by porcine epidemic diarrhea virus (PEDV) infection, comprising administering the Fuzheng Jiedu recipe or the above-mentioned pharmaceutical composition to an individual in need thereof, such as a pig.

[0021] The inventors have discovered that the Fuzheng Jiedu Recipe, kaempferol-7-O-glucoside, acladine, kaempferol, and octyl gallate have excellent inhibitory activity against porcine epidemic diarrhea virus (PEDV). Therefore, the Fuzheng Jiedu Recipe, kaempferol-7-O-glucoside, acladine, kaempferol, and octyl gallate of the present invention have important clinical application value for the treatment of diseases caused by porcine epidemic diarrhea virus (PEDV) infection.

[0022] The inventors found that Fuzheng Jiedu Recipe and kaempferol-7-O-glucoside, aclaidine, kaempferol, and octyl gallate have good inhibitory activity against porcine epidemic diarrhea virus (PEDV). 50 =0.4500mg / ml, CC 50 =7.499 mg / ml, SI=16.66; EC of compound Kaempferol-7-O-Glucoside against PEDV on Vero E6 cells 50 =0.41μM, CC 50 >100μM, SI>243.90; EC value of icaritin against PEDV in Vero E6 cells 50 =2.69μM, CC 50 >100μM, SI>37.17; EC value of Kaempferol against PEDV on Vero E6 cells 50 =3.22μM, CC 50 >100μM, SI>31.06; EC value of octyl gallate against PEDV on Vero E6 cells 50 =0.86μM, CC 50 =20.72 μM, SI=24.09. The above results indicate that Fuzheng Jiedu Recipe and kaempferol-7-O-glucoside, aclaidine, kaempferol, and octyl gallate have great potential clinical application value in preventing, alleviating and / or treating porcine diarrhoea virus infectious diseases. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 This is the test result of Fuzheng Jiedu prescription inhibiting PEDV virus infection.

[0024] Figure 2 Figure 2 shows the test results of Fuzheng Jiedu Recipe at different stages of action on PEDV-infected Vero E6 cells (**** indicates significant difference between the experimental group and the control group, P<0.0001).

[0025] Figure 3The graph shows the test results of the compounds kaempferol-7-O-glucoside, icaritin, kaempferol, and octyl gallate inhibiting PEDV virus infection.

[0026] Figure 4 The figure shows the test results of different stages of the action of compounds kaempferol-7-O-glucoside, icaritin, kaempferol, and octyl gallate on PEDV-infected VeroE6 cells (wherein, * indicates that there is a significant difference between the experimental group and the control group, P<0.05; ** indicates that there is a significant difference between the experimental group and the control group, P<0.01; *** indicates that there is a significant difference between the experimental group and the control group, P<0.001; **** indicates that there is a significant difference between the experimental group and the control group, P<0.0001). DETAILED DESCRIPTION

[0027] The technical solutions of the present invention will be described in further detail below with reference to specific embodiments. It should be understood that the following embodiments are merely illustrative and explanations of the present invention and should not be construed as limiting the scope of protection of the present invention. All technologies implemented based on the above content of the present invention are encompassed within the scope of protection that the present invention is intended to protect.

[0028] Unless otherwise specified, the raw materials and reagents used in the following examples are commercially available or can be prepared by known methods.

[0029] The preparation method of the Fuzheng Jiedu Fang granules used in the following examples is as follows: 100g of Radix Aconiti Lateralis Preparata, 15g of dried ginger, 20g of roasted liquorice root, 10g of honeysuckle, 10g of thorny Chinese honeysuckle, 20g of five-fingered peach, 10g of patchouli, and 5g of dried tangerine peel. The above prescription is added with 8 times the weight of water and decocted three times, each time for 1 hour. The decoction is filtered, the filtrates are combined, and concentrated under reduced pressure at 80°C (vacuum degree is -0.090 to -0.1MPa) to form an extract (each gram of extract is equivalent to approximately 2g of the slices). 0.5% (mass fraction) of stevia and appropriate amounts of lactose-dextrin (mass ratio is 2:1) are added, and granulated in one step to prepare 1000g of granules, which are packaged separately.

[0030] Example 1 Antiviral Activity Test of Fuzheng Jiedu Granules

[0031] 1. Fuzheng Jiedu Fang Granules inhibit porcine epidemic diarrhea virus (PEDV)

[0032] 1) Cell virus culture

[0033] Vero E6, an African green monkey kidney cell line, was obtained from the American Type Culture Collection (ATCC, No. 1586) and cultured in DMEM medium (Gibco) containing 10% fetal bovine serum (FBS; Gibco Invitrogen) at 37°C in a 5% CO2 incubator.

[0034] 2) Fuzheng Jiedu Recipe inhibits the EC of porcine epidemic diarrhea virus (PEDV) 50 and CC 50 Determination

[0035] EC 50 Detection: 2.5×10 4 Vero E6 cells were seeded into 96-well plates and cultured in a 37°C, 5% CO2 incubator for 24 h. Fuzheng Jiedufang granules with final concentrations of 12.5 mg / mL, 6.25 mg / mL, 3.125 mg / mL, 1.56 mg / mL, 0.78 mg / mL, 0.39 mg / mL, 0.195 mg / mL, 0.098 mg / mL, and 0 mg / mL were added to the cell culture wells and pre-incubated at 37°C for 1 h. The cells were preincubated with Fuzheng Jiedufang granules at 4°C for 1 hour. After incubation, the cells were infected with virus at a final MOI of 0.01. After 2 hours of infection, the cells were washed three times with PBS and the medium was replaced. Medium containing only the drug was added to final concentrations of 12.5 mg / mL, 6.25 mg / mL, 3.125 mg / mL, 1.56 mg / mL, 0.78 mg / mL, 0.39 mg / mL, 0.195 mg / mL, 0.098 mg / mL, and 0 mg / mL, respectively. The cells were cultured in a 37°C, 5% CO2 incubator. Cytopathic effects were observed microscopically 48 hours after PEDV infection, and the expression of intracellular viral RNA and the reference gene GAPDH was quantified by qRT-PCR. GraphPad-Prism 8 software was used for data analysis and calculation of EC values. 50 .

[0036] CC 50 Detection: CC was performed using the CellTiter-Blue method 50Vero E6 cells were seeded into 96-well cell culture plates, and the test was carried out when the cell density reached 60%-80%. After the Vero E6 cells were replaced with diluted drugs, the final concentrations were 12.5 mg / mL, 6.25 mg / mL, 3.125 mg / mL, 1.56 mg / mL, 0.78 mg / mL, 0.39 mg / mL, 0.195 mg / mL, 0.098 mg / mL, and 0 mg / mL, respectively. The cells were cultured at 37°C with 5% CO2 for 48 hours, and the luminescence intensity at 593 nm was detected using CellTiter-Blue reagent. The data were analyzed and calculated using GraphPad-Prism 8 software. 50 .

[0037] 3) Dosing time experiment

[0038] 1×10 5 Vero E6 cells were seeded into 24-well plates and cultured in a 37°C, 5% CO₂ incubator for 24 hours. Fuzheng Jiedu Fang granules were added to the wells for the entire period and before cell entry, respectively, at a final concentration of 3 mg / mL. The cells were pre-incubated at 37°C for 1 hour. Simultaneously, the virus was pre-incubated with Fuzheng Jiedu Fang granules at a final concentration of 3 mg / mL at 4°C for 1 hour. Following incubation, the cells were infected with virus at a final MOI of 0.01. Two hours after infection, the cells were washed three times with PBS and the medium was replaced. Medium containing only the drug was added to the wells for the entire period and after cell entry, while medium without the drug was added to the wells for the pre-infection and control groups at a final concentration of 3 mg / mL. The cells were incubated in a 37°C, 5% CO₂ incubator. Ten hours after viral infection, the expression of viral RNA and the cellular reference gene GAPDH was quantified by qRT-PCR. Data were analyzed using GraphPad-Prism 8 software.

[0039] 4) Virus sample processing and testing

[0040] RNA was extracted using the Axygen™ Multipurpose Total RNA Mini Kit (Axygene, Catalog No. AP-MN-MS-RNA-250G) according to the manufacturer's instructions. Reverse transcription was performed using the Hifair II 1st Strand cDNA Synthesis Kit (Shanghai Yisheng Biotechnology, Catalog No. 11121ES60) with gDNA digestion. Quantstudio Real-Time PCR Detection Reagents (Applied Biosystems, Foster City, CA, USA) were used for qRT-PCR amplification using the SYBR Green method: 95°C for 5 min, followed by 40 cycles of 95°C for 10 s, 55°C for 20 s, and 72°C for 31 s. Normalization was achieved by detecting the GAPDH gene.

[0041] 2. Kaempferol-7-O-glucoside, acladine, kaempferol, and octyl gallate inhibit porcine epidemic diarrhea virus (PEDV)

[0042] 1) The above compounds inhibit the EC of porcine epidemic diarrhea virus (PEDV) 50 and CC 50 Determination

[0043] EC 50 Detection: 2.5×10 4 Vero E6 cells were seeded into 96-well plates and cultured in a 37°C, 5% CO2 incubator for 24 h. Kaempferol-7-O-glucoside with a final concentration of 100 μM, 50 μM, 25 μM, 12.5 μM, 6.25 μM, 3.125 μM, 1.56 μM, 0.78 μM, and 0 μM, or octyl gallate with a final concentration of 50 μM, 25 μM, 12.5 μM, 6.25 μM, 3.125 μM, 1.56 μM, 0.78 μM, 0.39 μM, and 0 μM, respectively, were added to the cell culture wells. Kaempferol or aclaidine at concentrations of 25 μM, 12.5 μM, 6.25 μM, 3.125 μM, 1.56 μM, 0.78 μM, 0.39 μM, 0.20 μM, and 0 μM were pre-incubated at 37°C for 1 hour. At the same time, PEDV virus was pre-incubated with the corresponding final concentration of the above compounds at 4°C for 1 hour. After the incubation, the cells were infected with the virus at a final MOI of 0.01. After 2 hours of infection, the cells were washed three times with PBS and the medium containing only the corresponding final concentration of the drug was added. The cells were cultured in an incubator at 37°C and 5% CO2. 48 hours after PEDV virus infection, the cell lesions were observed under a microscope, and the expression of intracellular viral RNA and the cellular reference gene GAPDH was quantitatively detected by qRT-PCR. GraphPad-Prism 8 software was used for data analysis and calculation of EC 50 .

[0044] CC 50 Detection: CC was performed using the CellTiter-Blue method 50 Vero E6 cells were seeded into 96-well cell culture plates and the test was performed when the cell density reached 60%-80%. After the Vero E6 cells were replaced with diluted drugs, the final concentrations were 50 The cells were cultured at 37°C with 5% CO2 for 48 h, and the luminescence intensity at 593 nm was detected using CellTiter-Blue reagent. The data were analyzed and CC was calculated using GraphPad-Prism 8 software. 50 .

[0045] 2) Dosing time experiment

[0046] 1×10 5 Vero E6 cells were seeded into 24-well plates and cultured in a 37°C, 5% CO2 incubator for 24 h. Then, 6.25 μM kaempferol-7-O-glucoside / 12.5 μM icaritin / 12.5 μM kaempferol / 6.25 μM octyl gallate were added to the cell culture wells at all times and before cell entry, respectively. The cells were pre-incubated at 37°C for 1 h. At the same time, the virus was incubated with 6.25 μM kaempferol-7-O-glucoside / 12.5 μM icaritin / 12.5 μM kaempferol / 6.25 μM octyl gallate. The cells were preincubated with 1 μg of kaempferol-7-O-glucoside (6.25 μM kaempferol-7-O-glucoside), 12.5 μM icaritin, 12.5 μM kaempferol, and 6.25 μM octylgallate at 4°C for 1 hour. After infection, the cells were infected with virus at a final MOI of 0.01. Two hours after infection, the cells were washed three times with PBS and the medium was replaced. Drug-only medium was added to the wells during the entire period and after cell entry. Drug-free medium was added to the wells before cell entry and in the control group. The cells were cultured in a 37°C, 5% CO2 incubator. Ten hours after infection, the expression of viral RNA and the cellular reference gene GAPDH was quantified by qRT-PCR. Data were analyzed using GraphPad-Prism 8 software.

[0047] 3) Virus sample processing and testing

[0048] RNA was extracted using the Axygen™ Multipurpose Total RNA Mini Kit (Axygene, Catalog No. AP-MN-MS-RNA-250G) according to the manufacturer's instructions. Reverse transcription was performed using the Hifair II 1st Strand cDNA Synthesis Kit (Shanghai Yisheng Biotechnology, Catalog No. 11121ES60) with gDNA digestion. Quantstudio Real-Time PCR Detection Reagents (Applied Biosystems, Foster City, CA, USA) were used for qRT-PCR amplification using the SYBR Green method: 95°C for 5 min, followed by 40 cycles of 95°C for 10 s, 55°C for 20 s, and 72°C for 31 s. Normalization was achieved by detecting the GAPDH gene.

[0049] Experimental results

[0050] See the results Figure 1 ,Depend on Figure 1 It can be seen that different concentrations of Fuzheng Jiedufang granules have a good inhibitory effect on PEDV, and have a dose-dependent effect.

[0051] In addition, by Figure 1 It can be seen that the EC of Fuzheng Jiedufang granules against PEDV on Vero E6 cells 50 =0.4500mg / mL, CC 50 =7.499 mg / mL, SI=16.66.

[0052] Depend on Figure 2 It can be seen that the Fuzheng Jiedu recipe, at a concentration of 3 mg / mL, has an inhibitory effect on PEDV-infected VeroE6 cells before, after and throughout the cell cycle.

[0053] Depend on Figure 3 It can be seen that the above compounds at different concentrations have a good inhibitory effect on PEDV and have a dose-dependent effect.

[0054] In addition, by Figure 3 It can be seen that Kaempferol-7-O-Glucoside has an EC value of 1.577 for PEDV in Vero E6 cells. 50 =0.41μM, CC 50 >100μM, SI>243.90; EC of icaritin against PEDV 50 =2.69μM, CC 50 >100μM, SI>37.17; EC of Kaempferol against PEDV 50 =3.22μM, CC 50>100μM, SI>31.06; EC of octyl gallate against PEDV 50 =0.86μM, CC 50 =20.72μM, SI=24.09.

[0055] Depend on Figure 4 It can be seen that kaempferol-7-O-Glucoside (Kaempferol-7-O-Glucoside) at a concentration of 6.25 μM has an inhibitory effect on PEDV infection of VeroE6 cells before, after and during the entire period; icaritin (Icaritin) at a concentration of 12.5 μM has an inhibitory effect on PEDV infection of VeroE6 cells before, after and during the entire period; kaempferol (Kaempferol) at a concentration of 12.5 μM has an inhibitory effect on PEDV infection of VeroE6 cells before, after and during the entire period; octyl gallate (Octyl gallate) at a concentration of 6.25 μM has an inhibitory effect on PEDV infection of VeroE6 cells before, after and during the entire period.

[0056] In summary, the inventors of this invention have demonstrated that Fuzheng Jiedu Fang granules and the aforementioned compounds exhibited strong dose-dependent antiviral efficacy and low cytotoxicity against porcine epidemic diarrhea virus (PEDV) infection in Vero E6 cells. Currently, there are no specific drugs for PEDV, so this invention holds important implications for the future clinical use of PEDV.

[0057] Given the observed strong inhibitory effects of Fuzheng Jiedu Fang granules and the aforementioned compounds against PEDV, they are highly likely to become effective treatments for porcine epidemic diarrhea. The inventors believe that Fuzheng Jiedu Fang granules and the aforementioned compounds possess significant medicinal value as potential treatments for PEDV infection and represent promising drug candidates for the treatment of porcine epidemic diarrhea virus infections.

[0058] The above describes the embodiments of the present invention. However, the present invention is not limited to the above embodiments. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention shall be included in the scope of protection of the present invention.

Claims

1. Use of a Fuzheng Jiedu prescription and at least one of the following compounds in a medicament for preventing, alleviating and / or treating diseases caused by porcine epidemic diarrhea virus: kaempferol-7-O-glucoside, acladine, kaempferol, octyl gallate.

2. The use according to claim 1, characterized in that The Fuzheng Jiedu prescription is composed of the following 8 Chinese medicinal herbs in parts by mass: 10 parts of Radix Aconiti Lateralis Preparata, 15 parts of dried ginger, 20 parts of roasted licorice root, 10 parts of honeysuckle, 10 parts of honeysuckle thorn, 20 parts of five-fingered ginseng, 10 parts of patchouli, and 5 parts of dried tangerine peel, totaling 100 parts.

3. The use according to claim 2, characterized in that The Fuzheng Jiedu prescription was formulated by Academician Liu Liang and his team based on the general symptoms of patients infected with the Delta strain.

4. The use according to claim 2 or 3, characterized in that The Fuzheng Jiedu prescription is a Fuzheng Jiedu prescription granule, and its preparation method is as follows: add 8 times the weight of water to the prescription, decoct three times, each decoction is 1 hour, filter the decoction, combine the filtrate, and concentrate under reduced pressure at 80°C to form an extract, add stevia with a mass fraction of 0.5% and an appropriate amount of lactose-dextrin with a mass ratio of 2:1, granulate in one step, make 1000g of granules, and package them.

5. The use according to claim 4, characterized in that The vacuum degree used for reduced pressure concentration is -0.090 to -0.1 MPa.

6. The use according to claim 4, characterized in that Each gram of extract is equivalent to 2g of medicinal pieces.

7. The use according to claim 1, characterized in that The porcine epidemic diarrhea virus is inhibited at the organism level or the cell level.

8. The use according to claim 1, characterized in that The symptoms caused by porcine epidemic diarrhea virus infection are diarrhea, vomiting, or dehydration.

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