Detachable fluorescence detection enhanced detection pen and detection method

Abstract

The invention provides a detachable fluorescence detection enhanced detection pen and a detection method, and relates to the technical field of medical instruments. An upper shell and a lower shell are buckled to form an equipment shell; the control panel comprises a light path system, the light path system comprises a plurality of lamp beads arranged on the control panel, the lamp beads respectively emit light with different excitation light wavelengths, and the control panel is fixedly installed between the upper shell and the lower shell; the detection card insertion channel is arranged on one side of the equipment shell, detachably connected detection test paper is arranged on the detection card, and the detection card is detachably connected with the detection pen through the detection card insertion channel; the result observation window is arranged at the position, corresponding to the light path system, of the upper shell, the light filtering element is arranged on the result observation window, the light filtering element can make target light wavelength penetrate through the result observation window, the observation effect is clearer, judgment is easier, repeated use is convenient, the utilization rate is increased, and meanwhile the efficiency of multi-group detection conducted by a user is greatly improved.

Description

Technical Field

[0001] The invention belongs to the technical field of medical devices and relates to a detachable fluorescence detection enhanced detection pen and a detection method. Background Art

[0002] The fluorescent substance on the test strips in traditional test pens must reach a certain concentration to be visible to the naked eye. Therefore, the substance being tested must reach a certain concentration to trigger a reaction with the fluorescent substance and produce fluorescence. Currently, the test pens and test strips on the market primarily use colloidal gold. When used in situations such as early pregnancy, it cannot accurately measure results once the HCG concentration falls below a certain level. Furthermore, colloidal gold's anti-interference ability is inferior to other fluorescent materials, such as ordinary fluorescent microspheres and quantum dot fluorescent microspheres.

[0003] Reagent products using the colloidal gold method mostly use visual inspection of color to judge the results, and the identification and judgment of color will be subjective to a certain extent, especially when judging results near the critical value, which will cause interpretation difficulties for customers. Fluorescent reagents have higher sensitivity. Compared with current colloidal gold products, they have higher sensitivity and more advantages, and reflect better signals. In addition, fluorescence is used instead of color recognition, which is easier to interpret than naked eye recognition.

[0004] However, the existing technology still has the problem of stray light interference, which leads to poor observation effect. In actual detection, the concentration of the test object varies. When judging the test result near the critical value, that is, when the concentration of the test object is not high, the fluorescence effect is correspondingly not obvious. At this time, the customer is easily interfered by the stray light in the environment when observing the result, which will cause interpretation difficulties for the customer. At the same time, the existing technology is not efficient when multiple groups of tests are performed, and the use of multiple test pens causes waste of equipment and funds.

[0005] Chinese patent document CN107356767A discloses a luteinizing hormone test strip analyzer, which includes a reagent card and a test pen; the test pen includes a housing, a light-shielding cavity arranged in the housing, and a photoelectric detection system for color depth judgment. The housing of the test pen is provided with an insertion port. When the reagent card is inserted into the test pen through the insertion port, the detection window is correspondingly inserted into the light-shielding cavity. The photoelectric detection system collects reflected light during the color depth change of the test strip and converts the light signal into a digital electrical signal. The test result is automatically determined based on the threshold comparison, thereby improving the accuracy and efficiency of the measurement result, and realizing non-contact detection, avoiding contamination of the test pen. At the same time, by separating the reagent card and the test pen, the test pen can be recycled, saving detection costs. However, the test card used in this structure identifies and judges the test result by comparing color changes. The photoelectric detection system identifies the reflected light during the color depth change on the test card and then compares the results. This structure is easily affected by external ambient light and has weak anti-interference ability.

[0006] Chinese patent document CN102033129A discloses a test pen for detecting pathogenic microorganisms, comprising blood filter paper, colloidal gold pad, nitrocellulose membrane, absorbent filter paper and PVC bottom plate; the colloidal gold pad is coated with a mouse anti-human INF-γ monoclonal antibody-colloidal gold complex; the nitrocellulose membrane has a test line and a control line, the test line is coated with IFN-γ, and the control line is coated with a goat anti-mouse IgG polyclonal antibody; when the sample is dropped onto the absorbent stick, the IFN-γ in the sample moves with the solution to the colloidal gold pad and reacts with the colloidal gold pad. The gold-labeled IFN-γ monoclonal antibody combines to form an antigen-antibody complex. If there is not enough IFN-γ in the sample so that the gold-labeled IFN-γ monoclonal antibody can still bind to the antigen IFN-γ, then these monoclonal antibodies can bind to the IFN-γ at the T line when they move with the solution, making the T line red; conversely, if there is an excess of IFN-γ in the sample and the gold-labeled IFN-γ monoclonal antibody can no longer bind to the antigen IFN-γ, these monoclonal antibodies cannot bind to the IFN-γ at the T line when they move with the solution, and the T line will appear colorless. The gold-labeled IFN-γ monoclonal antibody that is not bound to the T line continues to move with the solution to the C line, and binds to the goat anti-mouse polyclonal antibody at the C line, turning the C line red, indicating that the mouse anti-human IFN-γ monoclonal antibody can be recognized by the goat anti-mouse polyclonal antibody. However, the nitrocellulose membrane used in this structure also obtains the test results by judging the color change. The nitrocellulose membrane has requirements for the concentration of the analyte in the sample. Once the concentration of the analyte is too low, it will be difficult to visually detect the color change of the nitrocellulose membrane.

[0007] Furthermore, existing detection equipment suffers from structural design issues, making it cumbersome and inefficient to operate, particularly when multiple tests are required. Furthermore, most detection equipment fails to adequately account for the interference of ambient light on test results. In fluorescence detection, in particular, effectively filtering stray light and enhancing the target fluorescence signal remains a technical challenge. Summary of the Invention

[0008] In response to the problems existing in the above-mentioned prior art, the present invention discloses a detachable fluorescence detection enhanced detection pen and detection method, which can block the interference of stray light, make the fluorescence effect more obvious, facilitate reuse, improve the utilization rate of the device, reduce waste, and improve the efficiency of users in performing multiple groups of detection.

[0009] To achieve the above-mentioned object, the present invention is implemented through the following technical solutions: a detachable fluorescence detection enhanced detection pen, comprising an upper shell, a lower shell, a control panel, a result observation window, a filter element and a detection card;

[0010] The upper shell and the lower shell are buckled together to form the device housing; the control board includes an optical path system, and the optical path system includes a plurality of lamp beads with different excitation light wavelengths arranged on the control board. The control board is fixedly installed between the upper shell and the lower shell; the detection card insertion channel is arranged on one side of the device housing, and a detection test paper is provided on the detection card. The detection card is detachably connected to the detection pen through the detection card insertion channel; the result observation window is arranged at a corresponding position between the upper shell and the optical path system, and the filter element is arranged on the result observation window. The filter element can allow the target light wavelength to pass through the result observation window.

[0011] Furthermore, the filter element is a transparent baffle, and the surface of the transparent baffle is coated.

[0012] Furthermore, the transparent baffle is made of acrylic, glass or PVC.

[0013] Furthermore: the filter element is a filter.

[0014] Furthermore: a protective cover is also included, and the protective cover is detachably connected to one side of the device housing.

[0015] Furthermore: it also includes a switch button, the switch button is arranged on the device housing, and the switch is electrically connected to the control panel.

[0016] Furthermore: an indicator light is also included, which is electrically connected to the control panel. When the switch button is pressed, the indicator light lights up, and when the switch button is released, the indicator light goes out.

[0017] Furthermore: it also includes a battery, which is installed in the device housing and is electrically connected to the control board.

[0018] Furthermore: it also includes a charging interface, which is a Type-c charging interface. The charging interface is arranged on the control board, and the charging interface passes through the device casing to communicate with the outside world.

[0019] Furthermore: the lamp beads in the optical path system are arranged along the edge of the result observation window.

[0020] Furthermore: the multiple lamp beads with different excitation light wavelengths include a specific wavelength light source that can excite the fluorescent substance on the test paper, and the fluorescent substance includes fluorescent microspheres, quantum dot fluorescent microspheres and time-resolved fluorescent microspheres.

[0021] A fluorescence detection method comprises the following steps:

[0022] S1: Insert the test card into the test card insertion channel;

[0023] S2: Start the optical path system on the control board;

[0024] S3: The lamp beads of the optical path system provide light of a specific wavelength to the test paper in the test card to excite the fluorescent substance on the test paper;

[0025] S4: Observe the test result of the test paper through a result observation window provided with a filter element.

[0026] Compared with the prior art, the present invention has the following beneficial effects:

[0027] First, the present invention effectively blocks stray light interference through the filter element, enhancing the fluorescence effect. Based on the actual excitation and emission wavelengths of different fluorescent substances, the filter element is modified to allow only specific wavelengths of light to pass through, while other wavelengths are absorbed by the material. This allows the desired target wavelength to specifically pass through the observation window, providing the operator with a clearer fluorescence signal. This is especially true when making judgments near critical values, resulting in clearer observations and easier accurate judgments.

[0028] Second, the present invention incorporates an optical system on the control panel, employing multiple lamps with different excitation wavelengths to provide precise excitation light sources for detection. These specific wavelengths effectively excite fluorescent substances in low-concentration samples, allowing the human eye to directly observe fluorescent signals even when the concentration of fluorescent substances in the test substance is low. This significantly improves the detection accuracy of the device, expands the detectable concentration range, and maintains high sensitivity even in low-concentration tests, offering significant advantages over traditional colloidal gold methods.

[0029] Third, this invention uses a fluorescent material instead of colloidal gold. Fluorescent materials offer higher sensitivity, better specificity, and a wider linear range of detection. By replacing color change with a fluorescent signal as the basis for judgment, the subjectivity of visually identifying color changes is reduced. Especially when the concentration of the test substance approaches the critical value, the fluorescent signal is significantly more discernible than the color change, improving the reliability and accuracy of the test results.

[0030] Fourth, the overall structural design of the present invention is compact and rational. The upper and lower shells snap together to form a complete device housing. A protective cover is provided at the bottom to protect the control panel. The operation interface is simple and intuitive, and the test card insertion channel structure is designed to facilitate the insertion and replacement of disposable reagent test cards. In addition, the Type-C charging port design facilitates charging and maintenance of the device, improving its ease of use and service life. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1 This is a schematic exploded view of a detachable fluorescence detection enhanced detection pen provided by the present invention;

[0032] Figure 2 A schematic perspective view of a detachable fluorescence detection enhanced detection pen provided by the present invention;

[0033] Figure 3 A schematic front view of the internal structure of a detachable fluorescence detection enhanced detection pen provided by the present invention;

[0034] Figure 4 A schematic side view of the internal structure of a detachable fluorescence detection enhanced detection pen provided by the present invention;

[0035] Figure 5 This is the actual effect diagram of the detection pen without coating;

[0036] Figure 6 This is the actual effect diagram of the detection pen using coating;

[0037] Figure 7 A flow chart of a fluorescence detection method provided by the present invention;

[0038] In the picture:

[0039] 1. Upper shell; 2. Lower shell; 3. Switch button; 4. Protective cover; 5. Test card; 6. Test paper; 7. Battery; 8. Control panel; 9. Result observation window; 10. Indicator light; 11. Charging port; 12. Lamp beads; 13. Filter element. DETAILED DESCRIPTION

[0040] The technical solution of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the embodiments described are only some embodiments of the present invention, not all embodiments. All other embodiments obtained by ordinary technicians in this field based on the embodiments of the present invention without making any creative efforts shall fall within the scope of protection of the present invention.

[0041] In the description of the present invention, it should be noted that the terms "center," "upper," "lower," "left," "right," "vertical," "horizontal," "inner," and "outer," etc., indicating orientations or positional relationships, are based on the orientations or positional relationships shown in the accompanying drawings and are intended solely to facilitate and simplify the description of the present invention. They are not intended to indicate or imply that the devices or components referred to must have, be constructed, or operate in a specific orientation, and therefore should not be construed as limitations on the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

[0042] Example 1

[0043] like Figures 1-4 The present invention provides a detachable fluorescence detection enhancement pen, comprising an upper housing 1, a lower housing 2, a control panel 8, a result viewing window 9, a filter element 13, and a test card 5. The unique design of the filter element 13 and its detachable structure effectively address the stray light interference and low detection efficiency issues found in existing testing equipment.

[0044] The upper shell 1 and the lower shell 2 are fastened together by means of snaps or screws to form a complete device housing, providing protection and support for the internal components. The device housing is made of durable engineering plastic material, and the surface is anti-slip treated to facilitate user grip and operation. The design of the upper shell 1 and the lower shell 2 takes into account ergonomic principles, making the test pen comfortable to hold and easy to operate. A result observation window 9 is provided on the surface of the upper shell 1 to facilitate the user to directly observe the test results. At the same time, corresponding grooves and slots are also designed on the upper shell 1 and the lower shell 2 to facilitate the installation and fixation of the control board 8 and other components.

[0045] The control board 8 is an important component of the detection pen and is fixedly installed between the upper shell 1 and the lower shell 2. The control board 8 uses a high-quality PCB board, on which the optical path system, circuit system and control system are integrated. The optical path system includes a plurality of lamp beads 12 with different excitation light wavelengths arranged on the control board 8. The plurality of lamp beads 12 can respectively emit light of different excitation light wavelengths. A single lamp bead 12 can emit light of a specific wavelength. The specific wavelength here refers to a single wavelength, which is used to excite the fluorescent substance on the test paper 6. The control board 8 is firmly installed between the upper shell 1 and the lower shell 2 through structures such as fixed columns and card slots to ensure that it will not loosen or shift during use. The control board 8 is also provided with a microprocessor for controlling the switching, brightness adjustment, power management and other functions of the lamp beads 12.

[0046] The test card insertion channel is provided on one side of the device housing and is an opening structure specifically used to insert the test card 5. The channel is designed with a guide groove and a positioning mechanism to ensure that the test card 5 can be accurately and stably inserted into the correct position. The test card 5 is a replaceable disposable component on which a test paper 6 is provided. The test paper 6 contains a specific fluorescent substance that can specifically bind to the substance being tested and generate a fluorescent signal. The test card 5 is detachably connected to the test pen through the test card insertion channel and can be easily taken out and replaced after use, thereby improving the reuse efficiency of the test pen. The design of the test card 5 adopts an anti-misinsertion structure, such as Figure 1 As shown, the detection card 5 is provided with a wide arc-shaped head on one side and a narrow square head on the other side, and the wide arc-shaped head is larger than the width of the detection card insertion channel. Even if the user tries to insert it by mistake, it cannot be inserted, ensuring that the user can only insert it in the correct direction to avoid operational errors.

[0047] It should be noted that the test card 5 contains the test paper 6, and what is read is the result of the test paper in the test card 5, but the test card 5 itself is not detachable for the user. What is provided to the user is the finished product after the test card 5 and the test paper 6 are assembled. What the user can detach during use is the combination of the test card 5 and the test paper 6.

[0048] The result observation window 9 is provided at a position corresponding to the optical path system of the upper shell 1 and is a window for the user to observe the test results. The result observation window 9 is made of a transparent material to ensure that the user can clearly observe the fluorescent signal on the test paper 6. The filter element 13 provided on the result observation window 9 is a key innovation of the present invention. The surface of the filter element 13 is coated. This special coating can block stray light of a specific wavelength and only allow light of a specific wavelength to pass through, thereby enhancing the clarity and visibility of the fluorescent signal. The implementation principle of this method belongs to the prior art.

[0049] The filter element 13 can be implemented in different ways:

[0050] One type is a transparent baffle with a coating. Available materials for transparent baffles include acrylic, glass, or PVC, all of which offer excellent light transmittance, stability, and durability. The choice of material is primarily based on factors such as cost, light transmission, and the operating environment. The coating on the surface of the transparent baffle is achieved through high-precision optical coating processes such as vacuum coating, enabling precise control of the wavelength range of transmitted light.

[0051] Another implementation method is to directly use a filter as the filter element 13. A filter is an optical component specifically designed to filter light of a specific wavelength. Its operating principle is similar to that of a coated transparent baffle, but the filtering effect is more specialized and precise. Filters are typically made of special optical glass or polymer materials and coated with multiple layers of interference film. They selectively transmit specific wavelengths of light based on the principle of interference between different wavelengths.

[0052] To protect the internal components of the pen, the present invention also includes a protective cover that is detachably connected to one side of the device housing. The cover is made of the same or similar material as the device housing and is attached to the housing via a snap or threaded connection. The design of the protective cover ensures the safety of the internal components while facilitating maintenance and cleaning.

[0053] The test pen also includes an on / off button 3, located on the device housing and electrically connected to the control panel 8. This button 3 employs a standard push-button design and is made of materials with excellent tactile feel and durability. By pressing this button, the user can turn the test pen on and off, making operation simple and intuitive. A spring mechanism is incorporated into the button to ensure good resilience and a comfortable feel.

[0054] To clearly indicate the operating status of the test pen, the present invention also includes an indicator light 10, which is electrically connected to the control board 8. When the switch button 3 is pressed, the indicator light 10 illuminates, indicating that the test pen is in the operating state; when the switch button 3 is released, the indicator light 10 turns off, indicating that the test pen is in the off state. The indicator light 10 uses a low-power LED light, typically green or blue, which clearly indicates the device status without interfering with the observation of test results.

[0055] To ensure the device's portability and independent operation, the present invention also includes a battery 7, which is installed within the device housing and electrically connected to a control board 8. Battery 7 utilizes a rechargeable lithium battery, which has a high energy density and a long service life. Battery 7 is secured within the device housing via a specially designed battery compartment, facilitating replacement and maintenance. The capacity of battery 7 is designed based on the device's power consumption and expected usage time, typically supporting several hours to several days of continuous use.

[0056] To facilitate charging of battery 7, the present invention also includes a Type-C charging port 11. This port is located on control board 8 and extends through the device housing to connect to the outside world. The Type-C charging port offers advantages such as direction independence, high transmission rates, and a compact size, making it a widely used charging port standard. A waterproof seal surrounds charging port 11 to prevent liquids from seeping into the device and causing damage.

[0057] The design of the optical path system is another important part of the present invention. The lamp beads 12 in the optical path system are arranged along the edge of the result observation window 9. This layout can provide uniform lighting and avoid the situation where local lighting is insufficient or too strong. The lamp beads 12 are fixed on the control board 8 using surface mount technology to ensure the consistency and stability of the lighting direction. The lamp beads 12 with multiple different excitation light wavelengths include specific wavelength light sources that can excite the fluorescent substances on the test paper 6. The fluorescent substances include fluorescent microspheres, quantum dot fluorescent microspheres and time-resolved fluorescent microspheres, preferably quantum dot fluorescent microspheres. These specific wavelength light sources are carefully selected and can effectively excite the fluorescent microspheres to produce fluorescent signals while avoiding unnecessary background interference.

[0058] Example 2

[0059] like Figure 7 As shown, the present invention also provides a fluorescence detection method, which is applied to the above-mentioned fluorescence detection enhanced detection pen and includes the following steps:

[0060] S1, insert the test card 5 into the test card insertion channel. The user first prepares the test card 5, which is pre-installed with a test paper 6 containing a specific fluorescent substance. Before testing, the user needs to add the sample to be tested to the sampling area of the test paper 6. Then, the user inserts the test card 5 along the guide groove of the test card insertion channel until the test card 5 reaches the preset position. At this time, a slight click feeling will be felt, indicating that the test card 5 has been correctly inserted.

[0061] S2: Start the optical system on the control panel 8. The user presses the on / off button 3 on the device housing, and the indicator light 10 lights up, indicating that the device is turned on. After receiving the on / off signal, the control panel 8 starts the optical system and prepares for the next step of testing.

[0062] S3, the light bead 12 of the optical path system provides light of a specific wavelength to the test paper 6 in the test card 5, stimulating the fluorescent substance on the test paper 6. Under the light, the fluorescent microspheres on the test paper 6 are excited. If the sample contains the substance to be detected, the fluorescent microspheres will bind to it and emit a fluorescent signal. The intensity of the fluorescent signal is proportional to the concentration of the substance to be detected.

[0063] The higher the concentration, the stronger the fluorescence signal; the lower the concentration, the weaker the fluorescence signal.

[0064] S4. The test results of the test strip 6 are observed through the result observation window 9 equipped with a filter element 13. The user observes the fluorescent signal on the test strip 6 through the result observation window 9. Due to the presence of the filter element 13, stray light is effectively blocked, and only fluorescence of a specific wavelength can pass through the filter element 13 and be observed by the user. This makes the fluorescent signal more clearly visible, especially when the concentration of the detected substance is low, and a good detection effect can be achieved. The user determines whether the test result is positive or negative based on the presence and intensity of the fluorescent signal.

[0065] Through the above steps, the fluorescence detection enhanced detection pen of the present invention can achieve rapid and accurate detection, especially when detecting low-concentration samples. After the test is completed, the user only needs to pull out the test card 5 and replace it with a new one to proceed with the next test, which is simple to operate and highly efficient.

[0066] The working principle of the present invention is as follows:

[0067] The working principle of the detachable fluorescence detection enhanced detection pen of the present invention is mainly based on fluorescence detection technology, and the detection signal is enhanced through the special filter element 13 and optical path system design. The working principle and functions of each component are described in detail below.

[0068] First, the core detection principle of this test pen is based on fluorescent immunoassay technology. The test strip 6 contains a fluorescent substance that fluoresces when exposed to light of a specific wavelength. When the sample contains the substance being tested, it reacts specifically with the antigen or antibody on the test strip 6, causing the fluorescent microspheres to aggregate at specific locations and produce a fluorescent signal. This signal can be observed with the naked eye under light of a specific wavelength, and the signal intensity is proportional to the concentration of the substance being tested.

[0069] The testing process begins with the user inserting the test card 5 into the test card insertion channel. The test card 5 is a disposable component containing a pre-installed test strip 6. The test card 5 is designed with a structure to prevent incorrect insertion, ensuring that it can only be inserted in the correct orientation. When the test card 5 is correctly inserted, the detection area on the test strip 6 is precisely aligned with the result viewing window 9, facilitating subsequent observation of the results.

[0070] When the user presses the on / off button 3, the control panel 8 receives the signal and starts operation. Control panel 8 is responsible for controlling the entire detection process. Upon receiving the on / off signal, the circuit system on control panel 8 immediately sends a working instruction to the optical system and illuminates indicator light 10, notifying the user that the device has started operation.

[0071] The optical system, a key component of the test pen, includes multiple lamp beads 12. These lamp beads 12 are positioned along the edge of the result viewing window 9 to provide uniform illumination. The specific wavelength of light emitted by these lamp beads 12 has been carefully selected to effectively stimulate the fluorescence of the fluorescent microspheres on the test strip 6. The specific wavelength here refers to a single wavelength. When light shines on the test strip 6, the fluorescent microspheres absorb the light energy of a specific wavelength and then emit fluorescence at another specific wavelength.

[0072] Under normal circumstances, the fluorescence signal may be interfered with by various stray lights. This is particularly true when the concentration of the substance being tested is low and the fluorescence signal is weak. This stray light interference can seriously affect the accuracy of the test results. This is where the filter element 13 of the present invention comes into play. The filter element 13 is positioned above the result observation window 9 and has a special coating treatment on its surface. This coating, based on the principle of interference in optical physics, selectively transmits light of specific wavelengths while blocking light of other wavelengths.

[0073] Specifically, when light of various wavelengths (including fluorescent signals and stray light) from the test strip 6 reaches the filter element 13, only light of the specific wavelength that matches the wavelength of light emitted by the fluorescent microspheres is able to pass through the filter element 13, while stray light of other wavelengths is absorbed or reflected by the filter element 13. This allows the user to view a purer and clearer fluorescent signal through the result viewing window 9, without interference from stray light.

[0074] The filter element 13 can be compared to an optical filter, filtering out stray light and retaining only the useful portion of fluorescence. This filtering effect is particularly important when the concentration of the detected substance is low and the fluorescence signal is weak, and can significantly improve the sensitivity and accuracy of detection.

[0075] There are two ways to implement filter element 13: one is a transparent barrier with a coating, which can be made of materials such as acrylic, glass, or PVC; the other is a dedicated filter. Both work on similar principles, selectively transmitting light of specific wavelengths, but filters generally provide more specialized and precise filtering effects.

[0076] The power supply system is essential for ensuring the proper function of the test pen. Battery 7 is installed in the device housing and electrically connected to the control board 8, providing power to the entire device. Battery 7 uses a rechargeable lithium battery with high energy density and a long service life. When battery 7 is low on power, the user can recharge it through the charging port 11. Charging port 11 is located on the control board 8 and connects to the outside world through the device housing for easy user operation. The Type-C port supports fast charging, significantly reducing charging time and improving device efficiency.

[0077] During the actual test process, the user first adds the sample to the sampling area of the test card 5 and then inserts the test card 5 into the test card insertion channel. After pressing the switch button 3, the optical system begins to operate, providing light of a specific wavelength to the test strip 6. The substance to be tested in the sample reacts with the reagent on the test strip 6, generating a fluorescent signal. The user observes the test results through the result observation window 9 equipped with a filter element 13 to determine whether the sample contains the substance to be tested and its approximate concentration.

[0078] After the test is completed, the user can release the switch button 3, the device stops working, and the indicator light 10 goes out. If a new test is needed, just pull out the used test card 5 and insert a new one, without having to replace the entire test pen, which greatly improves the test efficiency and equipment utilization.

[0079] The protective cover 4 is designed to protect the detection pen from the influence of the external environment when not in use. The protective cover 4 is detachably connected to one side of the device housing and can be removed when in use and re-covered when not in use to extend the service life of the device.

[0080] Compared to traditional colloidal gold detection methods, the fluorescence detection technology of the present invention has higher sensitivity and specificity. Traditional methods rely primarily on visual observation of color changes, which is inaccurate when detecting low-concentration samples and is easily influenced by subjective judgment. Fluorescence detection technology, on the other hand, uses the fluorescent signal to make judgments more objective and accurate, and has a clear advantage when detecting low-concentration samples.

[0081] At the same time, the detachable design of the present invention allows the detection pen to be reused, and only the detection card 5 needs to be replaced, which greatly reduces the detection cost and resource waste. This design is particularly suitable for scenarios where a large number of samples need to be tested, such as medical institutions, laboratories, and on-site testing.

[0082] The detachable fluorescence detection enhancement pen of the present invention effectively solves the problem of stray light interference in traditional fluorescence detection through the synergistic effect of the filter element 13 and the specific wavelength light source, making the fluorescent signal clearer and more discernible. The detachable design improves the efficiency and cost-effectiveness of the device and is suitable for various scenarios requiring high sensitivity and high specificity detection.

[0083] It should be noted that the surface of the filter element 13 is coated to achieve the principle and specific implementation process of blocking stray light: within the visible light range (400nm-750nm), after selecting the corresponding target wavelength range, the surface of the filter element 13 is coated. Its working principle is that when the light of the corresponding target wavelength range and the stray light of other wavelengths are transmitted to the coating at the same time, only the light of the corresponding target wavelength range can be transmitted through the coating, and the stray light of other wavelengths is absorbed by the coating, thereby reducing the interference of stray light transmitted from the filter element 13 inside the device, and the transmitted fluorescence effect is better, which plays a role in blocking the interference of stray light. The fluorescence effect of the light source is more obvious, and when judging the detection result near the critical value, the observation effect is clearer and easier to judge.

[0084] like Figure 5-Figure 6 The following are the actual effects of the detection pen without coating and with coating. Figure 5 This is the actual effect diagram of the detection pen without coating. Figure 6 This is the actual effect diagram of the coated detection pen. In actual detection, when judging the test result near the critical value, that is, when the concentration of the test object is not high, the fluorescence effect is correspondingly not obvious. At this time, the visual effect of the detection pen without coating is not obvious, while the detection pen with coating can see a clear line at the bottom of the detection window.

[0085] It should be noted that the material selected for the transparent baffle is one of acrylic, glass, and PVC, which has the effects of light transmission, fixation, sealing and convenient coating. The filter element 13 can choose to directly use a filter. The selected filter needs to be a filter for transmitting the selected target wavelength range. Its working principle is that when the light of the selected target wavelength range and the stray light of other wavelengths are transmitted to the filter at the same time, only the light of the selected target wavelength range can be transmitted through the filter, and the stray light of other wavelengths is absorbed by the filter, reducing the stray light interference transmitted from the inside of the device by the filter, and the transmitted fluorescence effect is better, further enhancing the effect of blocking stray light, and the fluorescence effect is more obvious, so that the required target light wavelength can specifically pass through the window, so that the operator can obtain a clearer fluorescence signal judgment. When judging the detection result near the critical value, the observation effect is more obvious and easier to judge.

[0086] The above embodiments are intended only to illustrate the technical concepts and features of the present invention. Their purpose is to enable those skilled in the art to understand the contents of the present invention and implement them accordingly. They are not intended to limit the scope of protection of the present invention. Any equivalent changes or modifications made in accordance with the spirit of the present invention are intended to be covered by the scope of protection of the present invention.

Claims

1. A detachable fluorescent detection enhanced detection pen, characterized by: It includes an upper shell, a lower shell, a control panel, a result observation window, a filter element and a test card; The upper shell and the lower shell are buckled together to form the device shell; the control board includes an optical path system, and the optical path system includes multiple lamp beads arranged on the control board, and the multiple lamp beads respectively emit light of different excitation light wavelengths, and the control board is installed between the upper shell and the lower shell; the detection card insertion channel is arranged on one side of the device shell, and a detection test paper is provided on the detection card, and the detection card is detachably connected to the detection pen through the detection card insertion channel; the result observation window is arranged at a corresponding position between the upper shell and the optical path system, and the filter element is arranged on the result observation window, and the filter element can allow the target light wavelength to pass through the result observation window.

2. The detachable fluorescence detection enhanced detection pen according to claim 1, characterized in that: The filter element is a transparent baffle, and the surface of the transparent baffle is subjected to coating treatment.

3. The detachable fluorescence detection enhanced detection pen according to claim 2, characterized in that: The transparent baffle is made of acrylic, glass or PVC.

4. The detachable fluorescence detection enhanced detection pen according to claim 1, characterized in that: The filter element is a filter.

5. The detachable fluorescence detection enhanced detection pen according to claim 1, characterized in that: It also includes a protective cover, which is detachably connected to one side of the device housing.

6. The detachable fluorescence detection enhanced detection pen according to claim 1, characterized in that: It also includes a switch button, which is arranged on the device housing, and the switch is electrically connected to the control panel.

7. The detachable fluorescence detection enhanced detection pen according to claim 6, characterized in that: It also includes an indicator light, which is electrically connected to the control panel. When the switch button is pressed, the indicator light lights up, and when the switch button is released, the indicator light goes out.

8. The detachable fluorescence detection enhanced detection pen according to claim 1, characterized in that: The device further comprises a battery, which is installed in the device housing and is electrically connected to the control board.

9. The detachable fluorescence detection enhanced detection pen according to claim 8, characterized in that: It also includes a charging interface, which is a Type-c charging interface. The charging interface is arranged on the control board, and the charging interface passes through the device casing to communicate with the outside world.

10. The detachable fluorescence detection enhanced detection pen according to claim 1, characterized in that: The lamp beads in the optical path system are arranged along the edge of the result observation window.

11. The detachable fluorescence detection enhanced detection pen according to claim 1, characterized in that: The multiple lamp beads include a wavelength light source that can excite the fluorescent substance on the test paper, and the fluorescent substance includes fluorescent microspheres, quantum dot fluorescent microspheres and time-resolved fluorescent microspheres.

12. A fluorescence detection method, characterized in that: The method uses the fluorescence detection enhanced detection pen according to any one of claims 1 to 11, comprising the following steps: S1: Insert the test card into the test card insertion channel; S2: Start the optical path system on the control board; S3: The lamp beads of the optical path system provide light of a specific wavelength to the test paper in the test card to excite the fluorescent substance on the test paper; S4: Observe the test result of the test paper through a result observation window provided with a filter element.

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