Bright field fluorescence integrated pathology slide scanning device and scanning method

Abstract

A brightfield fluorescence integrated pathology slide scanning device and scanning method includes: a white light source, a stage, an objective lens, a tube lens, and a black and white camera arranged sequentially along an optical path; a slide clamping device arranged on the side of the stage and movable relative to the stage; when the brightfield fluorescence integrated pathology slide scanning device is in brightfield scanning mode, the light beam emitted by the white light source passes through the stage, objective lens, and tube lens in sequence before being captured by the black and white camera; when the brightfield fluorescence integrated pathology slide scanning device is in fluorescence scanning mode, the light beam emitted by the white light source passes through a first filter, stage, objective lens, second filter, and tube lens in sequence before being captured by the black and white camera. This solution achieves the simultaneous use of brightfield imaging and fluorescence imaging on the same device by introducing metasurface technology on the camera surface and utilizing the characteristics of metasurface spectrum modulation. This eliminates the need to physically switch cameras and lighting methods, simplifies the device structure, and reduces operational complexity.

Description

Technical Field

[0001] The present invention relates to the field of pathology slide scanning, and in particular to a bright field fluorescence integrated pathology slide scanning device and a scanning method. Background Art

[0002] Traditional brightfield fluorescence slide scanning instruments usually split the brightfield and fluorescence functions into independent modules in terms of structural design: the brightfield scanning module uses transmissive illumination to collect the color and morphological information of the sample through a color camera; the fluorescence scanning module relies on epi-illumination, projecting excitation light from above to the sample, and then capturing the fluorescence signal through a black and white camera. In this design, fluorescence epi-illumination requires an independent light source and cannot be shared with the brightfield light source. In addition, the camera optical path and lighting mode must be converted synchronously when the modules are switched, resulting in a complex optical structure of the equipment and redundant mechanical components. In addition, the independent operation of the two modules requires image acquisition to be carried out in steps, and manual stitching or registration of brightfield and fluorescence images is required later. Not only is the operation process cumbersome, but it is also difficult to achieve efficient automated image processing, which restricts the throughput and analysis efficiency of slide scanning. Summary of the Invention

[0003] This solution provides an integrated brightfield and fluorescence pathology slide scanning device. By introducing metasurface technology on the camera surface and utilizing the characteristics of metasurface spectrum modulation, it achieves the functions of simultaneous brightfield imaging and fluorescence imaging on the same device. There is no need to physically switch cameras and lighting methods, which simplifies the device structure and reduces operational complexity.

[0004] To achieve the above objectives, this solution provides a brightfield fluorescence integrated pathology slide scanning device, including:

[0005] A white light source 1, a stage 2, an objective lens 3, a tube lens 4, and a black and white camera 5 are sequentially arranged along the optical path, wherein the lens surface of the camera is subjected to a super surface treatment;

[0006] A slide clamping device 6 is arranged at the side of the stage and is movable relative to the stage, wherein the slide clamping device 6 clamps a first filter 7 and a second filter 8 that are spaced apart;

[0007] When the brightfield fluorescence integrated pathology slide scanner is in brightfield scanning mode, the slide clamping device 6 is set away from the stage 2, and the light beam emitted by the white light source 1 passes through the stage 2, the objective lens 3, and the tube lens 4 in sequence and is captured by the black and white camera 5;

[0008] When the bright field fluorescence integrated pathology slide scanning device is in fluorescence scanning mode, the slide clamping device 6 is set close to the stage 2, and the light beam emitted by the white light source 1 passes through the first filter 7, the stage 2, the objective lens 3, the second filter 8, and the tube lens 4 in sequence and is captured by the black and white camera 5.

[0009] Preferably, when the bright field fluorescence integrated pathology slide scanning device is in fluorescence scanning mode, the first filter 7 is clamped to be located between the white light source 1 and the stage 2, the second filter 8 is clamped to be located between the objective lens 3 and the tube lens 4, and the first filter 7 and the second filter 8 are both located in the optical path.

[0010] Preferably, when the first filter 7 is between the white light source 1 and the stage 2, the first filter 7 is perpendicular to the exit optical axis of the light source 1, and the filtering area of ​​the first filter 7 is larger than the exit light beam diameter of the white light source 1; when the second filter 8 is between the objective lens 3 and the tube lens 4, the second filter 8 is perpendicular to the exit optical axis of the objective lens 3, and the filtering area of ​​the second filter 8 is larger than the exit light beam diameter of the objective lens 3.

[0011] Preferably, a metasurface modulation layer is covered on the surface of the image sensor of the black and white camera 5 , and the metasurface modulation layer is used to perform broadband modulation on the spectrum of the collected light beam to obtain spectral information of different frequency bands.

[0012] Preferably, the first filter 7 and the second filter 8 are multi-channel filters, and the excitation light emitted by the white light source 1 is filtered by the first filter 7 and then transmitted to the transparent pathology slide on the stage 2, wherein the first filter 7 filters the excitation light to obtain wavelength band light beams corresponding to different fluorescence channels, and the reflected light reflected by the transparent pathology slide passes through the objective lens 3, the second filter 8 and the tube lens 4 and is captured by the camera 5, wherein the second filter filters the reflected light and only retains the wavelength band light beam corresponding to the fluorescence channel.

[0013] This solution provides a scanning method for pathological slide samples, based on a brightfield fluorescence integrated pathology slide scanning device, including:

[0014] Placing a transparent pathology slide containing a pathology section sample to be scanned on the object stage 2;

[0015] Move the slide holding device 6 away from the stage 2 to set the triggering bright field scanning mode, turn on the white light source 1 to transmit the transparent pathology slide, and obtain the bright field scan image captured by the black and white camera 5, wherein the bright field scan image is modulated by the metasurface, and the spectrum reconstruction technology is used to perform spectral reconstruction on the bright field scan image to obtain a spectral image of the bright field scan;

[0016] The slide holder 6 is moved close to the stage 2 to set the trigger fluorescence scanning mode, the white light source 1 is turned on to transmit the transparent pathology slide, and the fluorescence scanning image captured by the black and white camera 5 is obtained. The fluorescence scanning image is modulated by the metasurface, and the fluorescence scanning image is spectrally separated using spectral separation technology to obtain a multi-channel fluorescence image;

[0017] Combine multi-channel fluorescence images and bright field scanning spectral images to obtain full spectrum imaging of the pathological section sample to be scanned;

[0018] Preferably, in the fluorescence scanning mode, at least one fluorescent dye is used to dye the pathological section sample to be scanned in the transparent pathological slide, and each fluorescent dye corresponds to a fluorescence channel.

[0019] Preferably, in the bright field scanning mode, the lens of the black and white camera 5 performs broadband modulation on the spectrum of the collected light beam to obtain a modulated light signal, and the modulated light signal is detected by the image sensor of the black and white camera 5 to obtain an intensity measurement value of each frequency band; in the fluorescence scanning mode, the lens of the black and white camera 5 performs broadband modulation on the collected spectrum to obtain a modulated light signal, and the modulated light signal is detected by the image sensor of the black and white camera 5 to obtain an intensity measurement value of each frequency band corresponding to the fluorescence channel.

[0020] Preferably, in the fluorescence scanning mode, the excitation light of the white light source 1 passes through the stage 2 and the first filter 7 in sequence and is divided into excitation light signals corresponding to different fluorescence channels. The reflected light reflected by the transparent pathology slide on the stage 2 passes through the objective lens 3, the second filter 8 and the tube lens 4 and is captured by the black and white camera 5 to obtain a multi-channel fluorescence image.

[0021] Preferably, in the bright field scanning mode, a linear equation group is used to solve the intensity measurement value of each frequency band to obtain the original spectral information, and a spectral image of the bright field scan is obtained based on the original spectral information; in the fluorescence scanning mode, spectral separation is used to separate the intensity measurement value of each frequency band corresponding to the fluorescence channel to obtain a multi-channel fluorescence image.

[0022] Compared with the existing technology, this technical solution has the following characteristics and beneficial effects:

[0023] This solution introduces metasurface technology on the camera surface and utilizes the characteristics of metasurface spectrum modulation to achieve the functions of simultaneous bright field imaging and fluorescence imaging on the same device. There is no need to physically switch the camera and lighting mode, which simplifies the device structure and reduces operational complexity. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 This is a schematic diagram of the structure of a bright field fluorescence integrated pathology slide scanning device in this scheme;

[0025] Figure 2 This is a schematic diagram of the structure of a bright field fluorescence integrated pathology slide scanning device in fluorescence scanning mode of this solution;

[0026] Figure 3 This is a schematic diagram of bright field imaging results obtained by a bright field fluorescence integrated pathology slide scanning device in this scheme;

[0027] Figure 4 This is a schematic diagram of the fluorescence imaging results obtained by the bright-field fluorescence integrated pathology slide scanning device in this scheme.

[0028] In the figure: 1- white light source, 2- stage, 3- objective lens, 4- tube lens, 5- black and white camera, 6- slide holding device, 7- first filter, 8- second filter. DETAILED DESCRIPTION

[0029] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. All other embodiments obtained by ordinary technicians in this field based on the embodiments of the present invention are within the scope of protection of the present invention.

[0030] It should be understood by those skilled in the art that, in the disclosure of the present invention, the terms "longitudinal", "transverse", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", etc., indicating the orientation or position relationship, are based on the orientation or position relationship shown in the accompanying drawings, which are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operate in a specific orientation. Therefore, the above terms should not be understood as limiting the present invention.

[0031] It is to be understood that the term "one" should be understood as "at least one" or "one or more", that is, in one embodiment, the number of an element may be one, while in another embodiment, the number of the elements may be multiple, and the term "one" should not be understood as a limitation on the quantity.

[0032] Example 1

[0033] This solution provides a bright field fluorescence integrated pathology slide scanning device such as Figure 1 Shown, including:

[0034] A white light source 1, a stage 2, an objective lens 3, a tube lens 4, and a black and white camera 5 are sequentially arranged along the optical path, wherein the lens surface of the camera is subjected to a super surface treatment;

[0035] A slide clamping device 6 is arranged at the side of the stage and is movable relative to the stage, wherein the slide clamping device 6 clamps a first filter 7 and a second filter 8 that are spaced apart;

[0036] When the brightfield fluorescence integrated pathology slide scanner is in brightfield scanning mode, the slide clamping device 6 is set away from the stage 2, and the light beam emitted by the white light source 1 passes through the stage 2, the objective lens 3, and the tube lens 4 in sequence and is captured by the black and white camera 5;

[0037] When the bright field fluorescence integrated pathology slide scanning device is in fluorescence scanning mode, the slide clamping device 6 is set close to the stage 2, and the light beam emitted by the white light source 1 passes through the first filter 7, the stage 2, the objective lens 3, the second filter 8, and the tube lens 4 in sequence and is captured by the black and white camera 5.

[0038] In some embodiments, the light source 1 is an LED white light source.

[0039] Specifically, since the white light source covers a wide spectral range, it can meet the needs of bright field scanning and fluorescence scanning at the same time.

[0040] Specifically, black and white cameras have higher photosensitivity and can obtain better imaging effects in both bright field scanning and fluorescence scanning modes. In particular, in fluorescence scanning mode, they can greatly reduce light loss and improve the signal-to-noise ratio of the image.

[0041] In some embodiments, a metasurface modulation layer is covered on the surface of the image sensor of the black and white camera 5 , and the metasurface modulation layer is used to perform broadband modulation on the spectrum of the collected light beam to obtain spectral information of different frequency bands.

[0042] This solution uses metasurface technology to process the lens of a black and white camera, so that images under brightfield scanning or fluorescence scanning can be obtained through spectral reconstruction or spectral separation operations. Therefore, when switching between brightfield scanning and fluorescence scanning, there is no need to switch the lighting method and imaging mode. One set of equipment can be used to perform brightfield scanning and fluorescence scanning on transparent pathology slides.

[0043] In some embodiments, when the bright field fluorescence integrated pathology slide scanning device is in fluorescence scanning mode, the first filter 7 is clamped to be located between the white light source 1 and the stage 2, the second filter 8 is clamped to be located between the objective lens 3 and the tube lens 4, and the first filter 7 and the second filter 8 are both located on the optical path.

[0044] In some embodiments, the first filter 7 and the second filter 8 are multi-channel filters. The excitation light emitted by the white light source 1 is filtered by the first filter 7 and then transmitted to the transparent pathology slide on the stage 2, wherein the first filter 7 filters the excitation light to obtain wavelength band light beams corresponding to different fluorescence channels, and the reflected light reflected by the transparent pathology slide passes through the objective lens 3, the second filter 8 and the tube lens 4 and is captured by the black and white camera 5, wherein the second filter filters the reflected light and only retains the wavelength band light beam corresponding to the fluorescence channel.

[0045] That is to say, the first filter 7 and the second filter 8 in this solution are used in a group, and the first filter 7 and the second filter 8 need to be replaced as the fluorescent dyes used in the pathological section samples are different.

[0046] For example, in the fluorescence scanning mode, the scanned pathological section sample uses four different fluorescent dyes, namely DAPI, FITC, CY3 and CY5. The first filter 7 in this scheme adopts a filter with model number Semrock S-000719 / FL-004249, which can divide the excitation light emitted by the light source 1 into four bands, which are used to excite the four different fluorescent dyes DAPI, FITC, CY3 and CY5 respectively. At the same time, the second filter 8 adopts a filter with model number Semrock S-000719 / FL-004445, which can filter out light in other bands and retain only the reflected light emitted by the stained part of the pathological section sample.

[0047] In some embodiments, when the first filter 7 is between the light source 1 and the stage 2, the first filter 7 is perpendicular to the output optical axis of the light source 1, and the filtering area of ​​the first filter 7 is larger than the output light beam diameter of the light source 1; when the second filter 8 is between the objective lens 3 and the tube lens 4, the second filter 8 is perpendicular to the output optical axis of the objective lens 3, and the filtering area of ​​the second filter 8 is larger than the output light beam diameter of the objective lens 3.

[0048] Specifically, the first filter 7 is perpendicular to the optical axis of the light source 1, and the second filter 8 is perpendicular to the optical axis of the objective lens 3. This ensures that the fluorescence signal enters the filter at an angle perpendicular to the filter, preventing refraction deviation or reduced filtering efficiency caused by oblique incidence. For example, if the filter is not perpendicular to the optical axis, the fluorescence signal may be blocked by the filter edge due to incident angle deviation, or the filtering bands of different wavelengths may shift, affecting the accuracy of subsequent spectral reconstruction. Furthermore, when the filter center coincides with the optical axis, the incident position of the fluorescence signal of each channel on the filter is relatively uniform, preventing excessive attenuation or cross-talk in a particular channel due to optical axis deviation. For example, if the filter center deviates from the optical axis, the long-wavelength fluorescence of the CY5 channel may enter the low-transmittance region at the filter edge, resulting in a decrease in the signal-to-noise ratio of the image in that channel and affecting the consistency of multi-channel imaging.

[0049] Specifically, the filtering area of ​​first filter 7 is larger than the diameter of the output beam of light source 1, and the filtering area of ​​second filter 8 is larger than the diameter of the output beam of objective lens 3. This ensures that after the white light source is banded by first filter 7, all excitation light can cover the sample area. At the same time, the fluorescence signal collected by the objective lens will not be blocked due to insufficient filter size when passing through second filter 8. For example, if the filtering area of ​​first filter 7 is smaller than the light source spot, the edge excitation light may directly illuminate the sample without being filtered, causing non-target band excitation light to interfere with the fluorescence signal and increase the risk of cross-color.

[0050] Specifically, as the objective lens magnification changes, the diameter of its output beam changes accordingly: for example, the beam diameter of a high-magnification objective lens is smaller, while that of a low-magnification objective lens is larger. The diameter of the second filter 8 is larger than the maximum output beam diameter of the objective lens, ensuring that the fluorescence signal fully passes through the filter at different magnifications, avoiding the need for optical path recalibration required when changing the objective lens. For example, when using a 40x objective lens, if the diameter of the second filter 8 only matches the beam diameter of a 20x objective lens, the edge fluorescence signal from the high-magnification objective lens may be blocked by the filter, resulting in uneven brightness at the edge of the image.

[0051] In some specific embodiments, the present solution controls the slide holding device 6 to slide by a sliding drive mechanism, and the slide holding device 6 moves back and forth in a direction perpendicular to the optical path.

[0052] Specifically, the sliding drive mechanism includes a stepper motor and a linear guide rail. The stepper motor drives the glass slide clamping device 6 through a gear rack transmission structure. The guiding accuracy of the linear guide rail is not less than 10 μm to ensure the position repeatability when the first filter 7 and the second filter 8 are switched.

[0053] In this scheme, the structure of the bright field fluorescence integrated pathology slide scanning device in the bright field scanning mode is as follows Figure 1 As shown, by controlling the slide clamping device 6 so that the first filter 7 and the second filter 8 are both outside the light path, the structure of the bright field fluorescence integrated pathology slide scanning device in the fluorescence scanning mode is as follows Figure 2 As shown, by controlling the slide clamping device 6, the first filter 7 is placed between the light source 1 and the stage 2, and the second filter 8 is placed between the objective lens 3 and the tube lens 4. Therefore, this solution can take into account the functions of bright field imaging and fluorescence imaging on the same device at the same time, without the need to physically switch the camera and lighting mode, simplifying the device structure and reducing the complexity of operation.

[0054] Example 2

[0055] A scanning method for pathological slide samples, based on a brightfield fluorescence integrated pathology slide scanning device, comprising:

[0056] Placing a transparent pathology slide containing a pathology section sample to be scanned on the object stage 2;

[0057] Move the slide holding device 6 away from the stage 2 to set the triggering bright field scanning mode, turn on the white light source 1 to transmit the transparent pathology slide, and obtain the bright field scan image captured by the black and white camera 5, wherein the bright field scan image is modulated by the metasurface, and the spectrum reconstruction technology is used to perform spectral reconstruction on the bright field scan image to obtain a spectral image of the bright field scan;

[0058] The slide holder 6 is moved close to the stage 2 to set the trigger fluorescence scanning mode, the white light source 1 is turned on to transmit the transparent pathology slide, and the fluorescence scanning image captured by the black and white camera 5 is obtained. The fluorescence scanning image is modulated by the metasurface, and the fluorescence scanning image is spectrally separated using spectral separation technology to obtain a multi-channel fluorescence image;

[0059] The full spectrum imaging of the pathological section sample to be scanned is obtained by combining the multi-channel fluorescence image and the spectral image of the bright field scan.

[0060] In some embodiments, in the fluorescence scanning mode, at least one fluorescent dye is used to dye the pathological section sample to be scanned in the transparent pathological slide, and each fluorescent dye corresponds to a fluorescence channel.

[0061] In some embodiments, in the bright field scanning mode, the lens of the black and white camera 5 performs broadband modulation on the spectrum of the collected light beam to obtain a modulated light signal, and the modulated light signal is detected by the image sensor of the black and white camera 5 to obtain an intensity measurement value of each frequency band; in the fluorescence scanning mode, the lens of the black and white camera 5 performs broadband modulation on the collected spectrum to obtain a modulated light signal, and the modulated light signal is detected by the image sensor of the black and white camera 5 to obtain an intensity measurement value of each frequency band corresponding to the fluorescence channel.

[0062] Specifically, the bright field imaging results obtained by the bright field fluorescence integrated pathology slide scanning device are as follows: Figure 3 As shown, the spectrum modulation method and the method for solving the linear equation used in this solution are existing technologies and will not be described here. Since the camera in this solution is a black and white camera, it can better receive the spectrum modulation results, thereby achieving richer imaging details.

[0063] In some embodiments, in the fluorescence scanning mode, the excitation light of the white light source 1 passes through the stage 2 and the first filter 7 in sequence and is divided into excitation light signals corresponding to different fluorescence channels. The reflected light reflected by the transparent pathology slide on the stage 2 passes through the objective lens 3, the second filter 8 and the tube lens 4 and is captured by the black and white camera 5 to obtain a multi-channel fluorescence image.

[0064] In some embodiments, in bright field scanning mode, a linear equation group is used to solve the intensity measurement value of each frequency band to obtain original spectral information, and a spectral image of the bright field scan is obtained based on the original spectral information; in fluorescence scanning mode, spectral separation is used to separate the intensity measurement value of each frequency band corresponding to the fluorescence channel to obtain a multi-channel fluorescence image.

[0065] Specifically, the fluorescence imaging results obtained by the bright field fluorescence integrated pathology slide scanning device are as follows: Figure 4 As shown, the fluorescence imaging result in this scheme is a multi-channel image, and each channel corresponds to the image of a different fluorescence channel. Through the precise spectral modulation of metasurface technology, this scheme can effectively distinguish between excitation light and emission light, avoiding the interference of excitation light in traditional drop-type illumination, and significantly improving the contrast and clarity of the image.

[0066] The present invention is not limited to the above-mentioned optimal implementation mode. Anyone can derive other forms of products under the inspiration of the present invention. However, no matter what changes are made in the shape or structure, any technical solution that is the same or similar to that of the present application falls within the scope of protection of the present invention.

Claims

1. A bright field fluorescence integrated pathology slide scanning device, characterized in that: include: A white light source (1), a stage (2), an objective lens (3), a tube lens (4), and a black and white camera (5) are sequentially arranged along an optical path, wherein the lens surface of the camera is subjected to a super-surface treatment; A glass slide clamping device (6) is arranged on the side of the stage and is movable relative to the stage, wherein the glass slide clamping device (6) clamps a first filter (7) and a second filter (8) that are spaced apart; When the bright field fluorescence integrated pathology slide scanning device is in bright field scanning mode, the slide clamping device (6) is set away from the stage (2), and the light beam emitted by the white light source (1) passes through the stage (2), the objective lens (3), and the tube lens (4) in sequence and is captured by the black and white camera (5); When the bright field fluorescence integrated pathology slide scanning device is in a fluorescence scanning mode, the slide clamping device (6) is set close to the stage (2), the first filter (7) is clamped to be located between the white light source (1) and the stage (2), the second filter (8) is clamped to be located between the objective lens (3) and the tube lens (4), and the first filter (7) and the second filter (8) are both located on the light path. When the first filter (7) is between the white light source (1) and the stage (2), the first filter (7) is perpendicular to the exit optical axis of the light source (1), and the filtering area of ​​the first filter (7) is larger than the exit beam diameter of the white light source (1). When the second filter (8) is between the objective lens (3) and the tube lens (4), the second filter (8) is perpendicular to the exit optical axis of the objective lens (3), and the filtering area of ​​the second filter (8) is larger than the exit beam diameter of the objective lens (3). The light beam emitted by the light source (1) passes through the first filter (7), the stage (2), the objective lens (3), the second filter (8), and the tube lens (4) in sequence and is captured by the black and white camera (5). The first filter (7) and the second filter (8) are multi-channel filters. The excitation light emitted by the white light source (1) is filtered by the first filter (7) and then transmitted to the transparent pathology slide on the stage (2). The first filter (7) filters the excitation light to obtain wavelength band light beams corresponding to different fluorescence channels. The reflected light reflected by the transparent pathology slide passes through the objective lens (3), the second filter (8) and the tube lens (4) and is captured by the black and white camera (5). The second filter filters the reflected light and only retains the wavelength band light beam corresponding to the fluorescence channel.

2. The bright field fluorescence integrated pathology slide scanning device according to claim 1, characterized in that: A metasurface modulation layer is covered on the surface of the image sensor of the black and white camera (5), and the metasurface modulation layer is used to perform broadband modulation on the spectrum of the collected light beam to obtain spectral information of different frequency bands.

3. A method for scanning a pathological slide sample, based on the bright field fluorescence integrated pathology slide scanning device according to any one of claims 1 to 2, characterized in that: include: Placing a transparent pathology slide containing a pathology section sample to be scanned on the object stage (2); The slide holding device (6) is moved away from the stage (2) to set a triggering bright field scanning mode, the white light source (1) is turned on to transmit the transparent pathology slide, and a bright field scanning image captured by the black and white camera (5) is obtained, wherein the bright field scanning image is modulated by the metasurface, and the spectrum reconstruction technology is used to perform spectrum reconstruction on the bright field scanning image to obtain a spectrum image of the bright field scanning; The slide holding device (6) is moved close to the stage (2) to set a trigger fluorescence scanning mode, the white light source (1) is turned on to transmit the transparent pathology slide, and a fluorescence scanning image captured by a black and white camera (5) is obtained, wherein the fluorescence scanning image is modulated by the metasurface, and the fluorescence scanning image is spectrally separated using spectral separation technology to obtain a multi-channel fluorescence image; The full spectrum imaging of the pathological section sample to be scanned is obtained by combining the multi-channel fluorescence image and the spectral image of the bright field scan.

4. The method for scanning a pathological section sample according to claim 3, wherein: In the fluorescence scanning mode, at least one fluorescent dye is used to dye the pathological section sample to be scanned in the transparent pathological slide, and each fluorescent dye corresponds to a fluorescence channel.

5. The method for scanning a pathological section sample according to claim 3, wherein: In the bright field scanning mode, the lens of the black and white camera (5) performs broadband modulation on the spectrum of the collected light beam to obtain a modulated light signal, and the modulated light signal is detected by the image sensor of the black and white camera (5) to obtain an intensity measurement value of each frequency band; in the fluorescence scanning mode, the lens of the black and white camera (5) performs broadband modulation on the collected spectrum to obtain a modulated light signal, and the modulated light signal is detected by the image sensor of the black and white camera (5) to obtain an intensity measurement value of each frequency band corresponding to the fluorescence channel.

6. The method for scanning a pathological section sample according to claim 3, wherein: In the fluorescence scanning mode, the excitation light from the white light source (1) passes through the stage (2) and the first filter (7) in sequence and is divided into excitation light signals corresponding to different fluorescence channels. The reflected light from the transparent pathology slide on the stage (2) passes through the objective lens (3), the second filter (8) and the tube lens (4) and is captured by the black and white camera (5) to obtain a multi-channel fluorescence image.

7. The method for scanning a pathological section sample according to claim 5, characterized in that: In the bright field scanning mode, the linear equations are used to solve the intensity measurement values ​​of each frequency band to obtain the original spectral information, and the spectral image of the bright field scan is obtained based on the original spectral information; in the fluorescence scanning mode, spectral separation is used to separate the intensity measurement values ​​of each frequency band corresponding to the fluorescence channel to obtain a multi-channel fluorescence image.

Images