Auxiliary device for in-vitro tissue magnetic resonance imaging

Abstract

The invention relates to the technical field of magnetic resonance imaging, and provides an auxiliary device for in-vitro tissue magnetic resonance imaging, which comprises a shell, a wrapping assembly and a sealing plug, the shell comprises a containing cavity and a packaging opening communicated with the containing cavity, the peripheral wall of the shell is configured to be matched with the inner wall of the scanning coil, and an MRI imaging medium is contained in the containing cavity; the wrapping assembly is arranged in the containing cavity and immersed in the MRI imaging medium, the wrapping assembly comprises a cavity and an opening communicated with the cavity, and the cavity is configured to be matched with the in-vitro tissue to be imaged in shape; the sealing plug is packaged in the packaging opening; wherein the MRI imaging medium is in a liquid state and is used for inhibiting external signal interference in the MRI imaging process of in-vitro tissues. The auxiliary device is easy and convenient to operate, interference of external signals can be reduced in the magnetic resonance imaging process, the imaging effect of the high signal-to-noise ratio of the special scanning coil is obtained, and the scientific research cost is effectively reduced.

Description

Technical Field

[0001] The present invention relates to the technical field of magnetic resonance imaging, and in particular to an auxiliary device for in vitro tissue magnetic resonance imaging. Background Art

[0002] As an advanced medical imaging technology, magnetic resonance imaging (MRI) has played a significant role in scientific research and disease monitoring. In biomedical research, MRI imaging of ex vivo tissues is of great significance in revealing pathological mechanisms, drug effects, and disease progression. MRI imaging technology does not require invasive procedures such as surgery or punctures. It is a non-invasive and non-destructive imaging technology that can fully display key information such as anatomical details and structural connections of tissues on multiple planes. In particular, in brain science research, MRI imaging can clearly display various regions and structures of the brain, and then accurately map the contours of various brain regions and structures, becoming an important technical means for researchers to conduct in-depth research on brain structure, brain function, and brain networks.

[0003] In practical applications, MRI imaging equipment is susceptible to external interference signals when using existing scanning coils to image ex vivo tissues, making it difficult to obtain high signal-to-noise ratio imaging. In order to improve the imaging quality of MRI imaging equipment, dedicated scanning coils are usually customized for the ex vivo tissue to be imaged. This not only increases scientific research costs, but also is impractical to equip each research tissue with a corresponding scanning coil, making it difficult to be widely used in scientific research. Summary of the Invention

[0004] The present invention provides an auxiliary device for in vitro tissue magnetic resonance imaging, which is used to at least solve or improve the problem that it is difficult to obtain high signal-to-noise ratio imaging of in vitro tissue in a low-cost manner based on existing MRI imaging equipment.

[0005] The present invention provides an auxiliary device for in vitro tissue magnetic resonance imaging, comprising: a housing, comprising a housing cavity and a packaging port communicating with the housing cavity, wherein a peripheral wall of the housing is configured to fit with an inner wall of the scanning coil, and an MRI imaging medium is contained in the housing cavity; a wrapping assembly disposed in the accommodating cavity and immersed in the MRI imaging medium, the wrapping assembly comprising a cavity and an opening communicating with the cavity, the cavity being configured to conform to a shape of an ex vivo tissue to be imaged; A sealing plug, the sealing plug being sealed in the sealing port; The MRI imaging medium is in liquid form and is used to suppress external signal interference during MRI imaging of the ex vivo tissue.

[0006] According to an auxiliary device for in vitro tissue magnetic resonance imaging provided by the present invention, the packaging component includes: A first inclusion body includes a first cavity formed on a first assembly surface of the first inclusion body; A second inclusion body includes a second cavity, wherein the second cavity is formed on a second assembly surface of the second inclusion body; The first assembling surface and the second assembling surface are fitted together, so that the first cavity and the second cavity are assembled into the mold cavity.

[0007] According to an auxiliary device for in vitro tissue magnetic resonance imaging provided by the present invention, both the first enclosure and the second enclosure are provided with the opening, and the side wall of at least one of the first enclosure and the second enclosure is further provided with a guide groove connected to the opening.

[0008] According to an auxiliary device for in vitro tissue magnetic resonance imaging provided by the present invention, the first enclosure and the second enclosure are both provided with a plurality of openings, and the plurality of openings are arranged side by side along the axial direction of the enclosure component; The first enclosure and the second enclosure are both provided with the guide groove, and the guide groove extends along the axial direction of the enclosure component.

[0009] According to the auxiliary device for in vitro tissue magnetic resonance imaging provided by the present invention, the first inclusion body further includes a first positioning portion, and the first positioning portion is provided on the first assembly surface; The second inclusion body further includes a second positioning portion, which is provided on the second assembling surface, and the first positioning portion and the second positioning portion are connected.

[0010] According to an auxiliary device for in vitro tissue magnetic resonance imaging provided by the present invention, the housing includes a bottom wall, the bottom wall is arranged opposite to the packaging opening, and the bottom wall is provided with a third positioning portion; The wrapping component includes a fourth positioning portion, which is provided at the first end of the wrapping component, and the third positioning portion is connected to the fourth positioning portion.

[0011] According to the auxiliary device for in vitro tissue magnetic resonance imaging provided by the present invention, the third positioning portion is a positioning hole, and the fourth positioning portion is a positioning pin, and the positioning pin is inserted into the positioning hole.

[0012] According to an auxiliary device for ex vivo tissue magnetic resonance imaging provided by the present invention, the wrapping assembly further includes an operating portion, which is provided at the second end of the wrapping assembly and abuts against the sealing plug.

[0013] According to the auxiliary device for in vitro tissue magnetic resonance imaging provided by the present invention, the operating part is configured as a "U"-shaped component.

[0014] According to the auxiliary device for in vitro tissue magnetic resonance imaging provided by the present invention, the MRI imaging medium includes fluorinated oil or superparamagnetic iron oxide nanoparticle solution.

[0015] The auxiliary device for magnetic resonance imaging of ex vivo tissue provided by the present invention is configured with a shell, a wrapping assembly and a sealing plug. The shell and the sealing plug cooperate to form a closed accommodating cavity. The ex vivo tissue can be placed in the cavity of the wrapping assembly. When the wrapping assembly is placed in the accommodating cavity, the wrapping assembly is immersed in the MRI imaging medium in the accommodating cavity. The MRI imaging medium can enter the cavity through the opening on the wrapping assembly and be immersed around the ex vivo tissue. In practical applications, there is no need to structurally modify the scanning coil of the existing MRI imaging equipment. The shell can be directly installed in the scanning coil to perform magnetic resonance imaging on the ex vivo tissue. Since the ex vivo tissue is immersed in the MRI imaging medium during the magnetic resonance imaging process, the MRI imaging medium can effectively isolate moisture and oxygen in the air, preventing oxidation and dehydration of the ex vivo tissue during the imaging process. The optical and physical properties of the MRI imaging medium can also be used to reduce interference from external signals, thereby obtaining an imaging effect with a high signal-to-noise ratio of the dedicated scanning coil, effectively reducing scientific research costs. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] In order to more clearly illustrate the technical solutions in the present invention or the prior art, a brief introduction is given below to the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0017] Figure 1 It is a schematic diagram of the three-dimensional structure of the auxiliary device for in vitro tissue magnetic resonance imaging provided by the present invention.

[0018] Figure 2 It is a schematic cross-sectional structural diagram of an auxiliary device for in vitro tissue magnetic resonance imaging provided by the present invention.

[0019] Figure 3 It is a schematic diagram of the top structure of the shell provided by the present invention.

[0020] Figure 4 It is a structural schematic diagram of the sealing plug provided by the present invention.

[0021] Figure 5 It is a schematic diagram of the main structure of the packaging assembly provided by the present invention.

[0022] Figure 6 It is a schematic diagram of the top view of the packaging assembly provided by the present invention.

[0023] Figure 7 It is a schematic diagram of the main structure of the first inclusion body provided by the present invention.

[0024] Figure 8 It is a schematic diagram of the bottom-up structure of the first inclusion body provided by the present invention.

[0025] Figure 9 It is a schematic diagram of the main structure of the second inclusion body provided by the present invention.

[0026] Figure 10 It is a schematic diagram of the top view of the second inclusion provided by the present invention.

[0027] Figure 11 This is a comparison diagram of MRI imaging of isolated mouse brain tissue provided by the present invention, wherein Figure 11 In the figure, (a) is an MRI image obtained by using the auxiliary device provided by the present invention, and (b) is an MRI image obtained without using the auxiliary device provided by the present invention.

[0028] Reference numerals: 1. Housing; 11. Accommodating cavity; 12. Packaging opening; 100. Third positioning portion; 2. Envelope assembly; 201. Cavity; 202. Opening; 203. Diversion groove; 21. First envelope; 210. First cavity; 211. First positioning portion; 22. Second envelope; 220. Second cavity; 221. Second positioning portion; 230. Fourth positioning portion; 240. Operating portion; 3. Sealing plug. DETAILED DESCRIPTION

[0029] To make the objectives, technical solutions, and advantages of the present invention more clear, the technical solutions of the present invention will be clearly and completely described below in conjunction with the accompanying drawings. Obviously, the embodiments described are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts shall fall within the scope of protection of the present invention.

[0030] The following combination Figures 1-11 , the auxiliary device for in vitro tissue magnetic resonance imaging provided by the embodiment of the invention is described in detail through specific embodiments and application scenarios.

[0031] In some embodiments, as Figure 1 、 Figure 2 、 Figure 3 and Figure 4As shown, an embodiment of the present invention provides an auxiliary device for in vitro tissue magnetic resonance imaging, comprising: a housing 1, a wrapping assembly 2 and a sealing plug 3; The housing 1 includes a housing cavity 11 and a sealing opening 12 communicating with the housing cavity 11. The peripheral wall of the housing 1 is configured to adapt to the inner wall of the scanning coil. The housing cavity 11 contains an MRI imaging medium. The packaging assembly 2 is disposed in the housing cavity 11 and immersed in the MRI imaging medium. The packaging assembly 2 includes a cavity 201 and an opening 202 communicating with the cavity 201. The cavity 201 is configured to adapt to the shape of the ex vivo tissue to be imaged. The sealing plug 3 is sealed in the sealing opening 12. Among them, the MRI imaging medium is in liquid form and is used to suppress external signal interference during MRI imaging of ex vivo tissue.

[0032] It is understood that for MRI imaging equipment, a channel is formed inside the scanning coil, and the shape of the peripheral wall of the housing 1 is adapted to the shape of the channel inside the scanning coil. The housing 1 is generally cylindrical, and the shape of the radial cross-section of the housing 1 is circular or elliptical.

[0033] For the wrapping component 2, by setting a cavity 201 and an opening 202 connected to the cavity 201, the ex vivo tissue that matches its shape can be placed based on the cavity 201, ensuring that the ex vivo tissue to be imaged is stably placed at the scanning center position of the scanning coil, ensuring the intensity and uniformity of the scanning signal, and effectively reducing the imaging difference introduced by the position difference of the ex vivo tissue in the scanning coil, and the opening 202 of the wrapping component 2 is used to connect the accommodating cavity 11 of the shell 1 and the cavity 201 of the wrapping component 2, ensuring that the MRI imaging medium in the accommodating cavity 11 can flow into the cavity 201 through the opening 202, ensuring that the ex vivo tissue is immersed in the MRI imaging medium for imaging, which is conducive to obtaining high signal-to-noise ratio imaging.

[0034] Among them, MRI imaging media include fluorinated oil or superparamagnetic iron oxide nanoparticle solution. MRI imaging media are used as contrast agents in magnetic resonance imaging, which can reduce interference from external signals, provide specific magnetic resonance signals, and improve imaging clarity and accuracy.

[0035] In practical applications, after wrapping assembly 2 is immersed in the MRI imaging medium within chamber 11, the assembly formed by housing 1 and wrapping assembly 2 can be evacuated to ensure that chamber 11 is in a vacuum state, thereby allowing bubbles in the MRI imaging medium to separate out. Sealing plug 3 is then sealed at sealing opening 12. This design prevents bubbles from remaining in the MRI imaging medium, thereby ensuring imaging quality. The size and distribution of openings 202 can be configured to correspond to the ex vivo tissue to be scanned, ensuring that the ex vivo tissue is completely immersed in the MRI imaging medium.

[0036] The auxiliary device shown in the embodiment of the present invention is configured with a shell 1, a wrapping component 2 and a sealing plug 3. The shell 1 and the sealing plug 3 cooperate to form a closed accommodating chamber 11, and the ex vivo tissue can be placed in the cavity 201 of the wrapping component 2. When the wrapping component 2 is placed in the accommodating chamber 11, the wrapping component 2 is immersed in the MRI imaging medium in the accommodating chamber 11. The MRI imaging medium can enter the cavity 201 through the opening 202 on the wrapping component 2 and be immersed around the ex vivo tissue. In actual application, there is no need to structurally modify the scanning coil of the existing MRI imaging equipment. The shell 1 can be directly installed in the scanning coil to perform magnetic resonance imaging on the ex vivo tissue. Since the ex vivo tissue is immersed in the MRI imaging medium during the magnetic resonance imaging process, the MRI imaging medium can effectively isolate moisture and oxygen in the air, preventing oxidation and dehydration of the ex vivo tissue during the imaging process. The optical and physical properties of the MRI imaging medium can also be used to reduce interference from external signals, thereby obtaining an imaging effect with a high signal-to-noise ratio of the dedicated scanning coil, effectively reducing scientific research costs.

[0037] At the same time, the auxiliary device shown in the embodiment of the present invention can ensure that the ex vivo tissue is in the scanning center position of the scanning coil during the scanning process. The placement and removal operation procedures of the ex vivo tissue are simple and can be completed without professional skills. It not only improves the efficiency of magnetic resonance imaging, but also provides an efficient and reliable solution for MRI imaging of ex vivo tissue in biomedical research.

[0038] In some embodiments, as Figure 5 、 Figure 8 and Figure 10 As shown, the wrapping assembly 2 includes: a first wrapping body 21 and a second wrapping body 22; The first inclusion body 21 includes a first cavity 210 formed on a first assembly surface of the first inclusion body 21 ; the second inclusion body 22 includes a second cavity 220 formed on a second assembly surface of the second inclusion body 22 ; The first assembling surface and the second assembling surface are fitted together, so that the first cavity 210 and the second cavity 220 are assembled into the mold cavity 201 .

[0039] It can be understood that by configuring the wrapping component 2 to include the first wrapping body 21 and the second wrapping body 22 , the placement and removal operations of the ex vivo tissue can be completed conveniently based on the assembly of the first wrapping body 21 and the second wrapping body 22 .

[0040] Among them, the first assembling surface of the first inclusion body 21 and the second assembling surface of the second inclusion body 22 are both planes, the first cavity 210 and the second cavity 220 are both groove-shaped, the inner wall of the first cavity 210 is adapted to the shape of the first side surface of the ex vivo tissue, and the inner wall of the second cavity 220 is adapted to the shape of the second side surface of the ex vivo tissue, so that the first cavity 210 and the second cavity 220 are assembled to form a closed cavity 201 that is adapted to the shape of the ex vivo tissue to be imaged, thereby ensuring the stability and reliability of the placement of the ex vivo tissue based on the cavity 201.

[0041] In some embodiments, as Figure 6 、 Figure 7 and Figure 9 As shown, both the first inclusion body 21 and the second inclusion body 22 are provided with an opening 202 , and a side wall of at least one of the first inclusion body 21 and the second inclusion body 22 is further provided with a guide groove 203 communicating with the opening 202 .

[0042] Specifically, the first inclusion body 21 also has a first side surface opposite to the first assembly surface, and the opening 202 on the first inclusion body 21 is opened on the first side surface, and the opening 202 on the first inclusion body 21 is connected to the first cavity 210 of the first inclusion body 21; accordingly, the second inclusion body 22 also has a second side surface opposite to the second assembly surface, and the opening 202 on the second inclusion body 22 is opened on the second side surface, and the opening 202 on the second inclusion body 22 is connected to the second cavity 220 of the second inclusion body 22.

[0043] At the same time, a guide groove 203 is also provided on the first side of the first inclusion body 21, and the guide groove 203 on the first inclusion body 21 is connected to the first cavity 210 through the opening 202 on the first inclusion body 21; a guide groove 203 is also provided on the second side of the second inclusion body 22, and the guide groove 203 on the second inclusion body 22 is connected to the second cavity 220 through the opening 202 on the second inclusion body 22.

[0044] Obviously, in this embodiment, by setting the guide groove 203, the MRI imaging medium in the accommodating cavity 11 can be guided to flow along the guide groove 203 through the guide groove 203, and then enter the cavity 201 through the opening 202. This design ensures that the MRI imaging medium in the accommodating cavity 11 can smoothly enter the cavity 201, and the peripheral wall of the wrapping component 2 can be set to fit as closely as possible with the inner wall of the shell 1, thereby ensuring the stability of the installation of the wrapping component 2 in the shell 1.

[0045] In some embodiments, as Figure 6 、 Figure 7 and Figure 9As shown, the first enclosure 21 and the second enclosure 22 can be provided with a plurality of openings 202, and the plurality of openings 202 are arranged side by side along the axial direction of the enclosure component 2; the first enclosure 21 and the second enclosure 22 are both provided with a guide groove 203, and the guide groove 203 extends along the axial direction of the enclosure component 2.

[0046] Thus, based on the above setting, the guide groove 203 on the first enclosure 21 is connected to the first cavity 210 through the multiple openings 202 on the first enclosure 21, and the guide groove 203 on the second enclosure 22 is connected to the second cavity 220 through the multiple openings 202 on the second enclosure 22. The first cavity 210 and the second cavity 220 are assembled into a cavity 201. This design can further ensure that the MRI imaging medium in the accommodating cavity 11 can smoothly enter the cavity 201.

[0047] In some embodiments, as Figures 7 to 10 As shown, the first inclusion body 21 further includes a first positioning portion 211, which is provided on the first assembly surface; the second inclusion body 22 further includes a second positioning portion 221, which is provided on the second assembly surface, and the first positioning portion 211 and the second positioning portion 221 are connected.

[0048] It is understandable that, according to the positioning provided by the first positioning portion 211 and the second positioning portion 221 , the first enclosure 21 and the second enclosure 22 can be assembled into one body in a certain posture, thereby ensuring the assembly efficiency of the first enclosure 21 and the second enclosure 22 .

[0049] Some examples, such as Figure 7 and Figure 9 As shown, the first positioning portion 211 may be a positioning hole, and the second positioning portion 221 may be a positioning post, which is inserted into the positioning hole.

[0050] Some examples, such as Figure 7 and Figure 9 As shown, in order to ensure the reliability and stability of the assembly of the first enclosure 21 and the second enclosure 22, a plurality of first positioning portions 211 and a plurality of second positioning portions 221 are provided, and the plurality of first positioning portions 211 and the plurality of second positioning portions 221 are arranged one by one relative to each other.

[0051] In some embodiments, as Figure 2 、 Figure 3 and Figure 5 As shown, the shell 1 includes a bottom wall, which is arranged opposite to the packaging port 12, and the bottom wall is provided with a third positioning portion 100; the wrapping component 2 includes a fourth positioning portion 230, and the fourth positioning portion 230 is arranged at the first end of the wrapping component 2, and the third positioning portion 100 and the fourth positioning portion 230 are connected.

[0052] It can be understood that the positioning provided by the third positioning portion 100 and the fourth positioning portion 230 can prevent the wrapping assembly 2 from rotating within the shell 1, thereby ensuring the stability of the placement of the ex vivo tissue during magnetic resonance imaging.

[0053] Some examples, such as Figure 2 As shown, the third positioning portion 100 is a positioning hole, and the fourth positioning portion 230 is a positioning pin. The positioning pin is inserted into the positioning hole, and a sealing connection is achieved between the peripheral wall of the positioning pin and the hole wall of the positioning hole through a sealing member.

[0054] Some examples, such as Figure 5 and Figure 7 As shown, in the case where the encapsulating assembly 2 includes a first encapsulating body 21 and a second encapsulating body 22 , the fourth positioning portion 230 is provided at the first end of the first encapsulating body 21 .

[0055] In some embodiments, as Figure 2 and Figure 5 As shown, the wrapping assembly 2 further includes an operating portion 240 , which is disposed at the second end of the wrapping assembly 2 and abuts against the sealing plug 3 .

[0056] It is understandable that after completing the imaging of the ex vivo tissue, the operator can apply force through the operating part 240 to remove the wrapping component 2 from the housing 1 .

[0057] The operating portion 240 is configured as a “U”-shaped component, and the “U”-shaped component can be disposed at the second end of the first enclosure 21 .

[0058] In some embodiments, the auxiliary device shown in the embodiments of the present invention can be used to perform MRI imaging of mouse brain tissue. The specific steps are as follows: Step 1: Fix the mouse brain tissue with 4% formaldehyde solution for 12 hours, then wash it in 1X PBS solution. After 30 minutes, remove the mouse brain tissue and wipe the liquid on the surface of the mouse brain tissue with absorbent paper; Step 2: Place the mouse brain tissue into the cavity of the packaging component, ensuring that the ventral side of the mouse brain tissue is in contact with the inner wall of the second cavity. The olfactory bulb of the mouse brain tissue is positioned toward the side of the "U"-shaped component. Pay attention to the orientation of the mouse brain tissue in the cavity to avoid placing the olfactory bulb in the wrong position. Step 3: Place the package assembly containing the mouse brain tissue into the housing cavity, ensuring that the third positioning portion and the fourth positioning portion are connected; Step 4: Pour fluorine oil into the receiving cavity until the package component is immersed in the fluorine oil; Step 5: vacuuming the assembly obtained in step 4 to remove any bubbles that may be present in the fluorine oil; Step 6: Place the sealing plug in the sealing port of the housing and seal the joint between the sealing plug and the housing with sealing tape to prevent the fluorine oil in the housing from leaking out. Step 7: MRI of the mouse brain tissue was performed using a Bruker BioSpec 94 / 30 small animal MRI imaging system with the following imaging parameters: repetition time TR = 2000 ms, echo time TE = 20 ms, average acquisition times Averages = 4, and field of view size FOV = 12 × 11 × 17 mm. 3 .

[0059] In actual operation, based on the MRI imaging system shown in step 7, the same mouse brain tissue is imaged using the auxiliary device provided by the present invention and without the auxiliary device provided by the present invention, and the following is obtained: Figure 11 Comparison of MRI images shown.

[0060] exist Figure 11 In the figure, (a) is an MRI image obtained using the auxiliary device provided by the present invention, which shows information such as the brain region structure and connections of the mouse brain. (b) is an MRI image obtained without the auxiliary device provided by the present invention. This image contains a large amount of background noise, and the image resolution is greatly reduced, making it almost impossible to see the connection information. Among them, (a) can clearly see the relevant structures of the corpus callosum in the mouse brain, while (b) cannot. Therefore, the signal-to-noise ratio of the MRI image shown in (a) is much higher than that of the MRI image shown in (b), which also shows that the auxiliary device shown in the embodiment of the present invention achieves an imaging effect with a high signal-to-noise ratio.

[0061] In summary, the auxiliary device for in vitro magnetic resonance imaging of tissues provided by the present invention can utilize the scanning coils and fluorinated oil of existing MRI imaging equipment to significantly improve the signal-to-noise ratio and resolution of imaging. Through the auxiliary device of the present invention, researchers can achieve high-quality MRI imaging of in vitro tissues without increasing equipment costs. This low-cost solution provides an innovative idea for scientific research and helps promote the further development of biomedical research.

[0062] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the various embodiments of the present invention.

Claims

1. An auxiliary device for in vitro tissue magnetic resonance imaging, characterized in that: include: a housing, comprising a housing cavity and a packaging port communicating with the housing cavity, wherein a peripheral wall of the housing is configured to fit with an inner wall of the scanning coil, and an MRI imaging medium is contained in the housing cavity; a wrapping assembly disposed in the accommodating cavity and immersed in the MRI imaging medium, the wrapping assembly comprising a cavity and an opening communicating with the cavity, the cavity being configured to conform to a shape of an ex vivo tissue to be imaged; A sealing plug, the sealing plug being sealed in the sealing port; The MRI imaging medium is in liquid form and is used to suppress external signal interference during MRI imaging of the ex vivo tissue.

2. The auxiliary device for in vitro tissue magnetic resonance imaging according to claim 1, characterized in that: The package assembly includes: A first inclusion body includes a first cavity formed on a first assembly surface of the first inclusion body; A second inclusion body includes a second cavity, wherein the second cavity is formed on a second assembly surface of the second inclusion body; The first assembling surface and the second assembling surface are fitted together, so that the first cavity and the second cavity are assembled into the mold cavity.

3. The auxiliary device for in vitro tissue magnetic resonance imaging according to claim 2, characterized in that: The first inclusion body and the second inclusion body are both provided with the opening, and the side wall of at least one of the first inclusion body and the second inclusion body is further provided with a guide groove communicating with the opening.

4. The auxiliary device for in vitro tissue magnetic resonance imaging according to claim 3, characterized in that The first enclosure and the second enclosure are both provided with a plurality of openings, and the plurality of openings are arranged side by side along the axial direction of the enclosure component; The first enclosure and the second enclosure are both provided with the guide groove, and the guide groove extends along the axial direction of the enclosure component.

5. The auxiliary device for in vitro tissue magnetic resonance imaging according to claim 2, characterized in that: The first inclusion body further includes a first positioning portion, and the first positioning portion is provided on the first assembly surface; The second inclusion body further includes a second positioning portion, which is provided on the second assembling surface, and the first positioning portion and the second positioning portion are connected.

6. The auxiliary device for in vitro tissue magnetic resonance imaging according to claim 1, characterized in that: The housing includes a bottom wall, the bottom wall is arranged opposite to the packaging opening, and the bottom wall is provided with a third positioning portion; The wrapping component includes a fourth positioning portion, which is provided at the first end of the wrapping component, and the third positioning portion is connected to the fourth positioning portion.

7. The auxiliary device for in vitro tissue magnetic resonance imaging according to claim 6, characterized in that: The third positioning portion is a positioning hole, and the fourth positioning portion is a positioning pin, which is inserted into the positioning hole.

8. The auxiliary device for in vitro tissue magnetic resonance imaging according to claim 6, characterized in that: The wrapping assembly further includes an operating portion, which is disposed at the second end of the wrapping assembly and abuts against the sealing plug.

9. The auxiliary device for in vitro tissue magnetic resonance imaging according to claim 8, characterized in that: The operating portion is configured as a "U"-shaped component.

10. The auxiliary device for in vitro tissue magnetic resonance imaging according to any one of claims 1 to 9, characterized in that: The MRI imaging medium includes fluorinated oil or superparamagnetic iron oxide nanoparticle solution.

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