Oral care composition containing cranberry extract and inulin and application thereof

Abstract

The invention discloses an oral care composition containing cranberry extract and inulin and application of the oral care composition, the oral care composition comprises 1) cranberry extract, 2) inulin and 3) an orally acceptable carrier, the mass ratio of cranberry extract in the oral care composition is 0.001-0.6%, the mass ratio of inulin in the oral care composition is 0.1-0.3%, and the mass ratio of inulin in the oral care composition is 0.1-0.3%. The mass ratio of the inulin in the oral care composition is 0.1-6%. The oral care composition disclosed by the invention has a growth promotion effect on non-pathogenic bacteria in the oral cavity, and also has no promotion or even inhibition effect on oral pathogenic bacteria, so that the oral flora environment can be effectively improved, and the oral health can be improved. The composition disclosed by the invention can also effectively inhibit bacteria from adhering to the oral cavity or the tooth surface.

Description

Technical Field

[0001] The present invention relates to the technical field of oral care, and in particular to an oral care composition comprising cranberry extract and inulin and application thereof. Background Art

[0002] Dental plaque is considered the primary cause of most oral diseases, including caries, gingivitis, and periodontitis. This is primarily due to the presence of numerous oral pathogens, which adhere to each other and firmly attach to the oral cavity and dental surfaces, making them difficult to remove. This can lead to oral problems such as caries, tartar, and oral inflammation. Common preventative measures include preventing bacterial adhesion to the oral cavity and dental surfaces, or balancing the oral flora by inhibiting the growth of oral pathogens and activating non-pathogenic bacteria, thereby reducing the number of oral pathogens in the biofilm and improving oral health. Summary of the Invention

[0003] The first technical problem addressed by the present invention is to provide an oral care composition containing cranberry extract and inulin. This composition effectively promotes the growth of non-pathogenic oral bacteria while having no promoting effect on, or even inhibiting, oral pathogens. By leveraging its differential effects on non-pathogenic and oral pathogenic bacteria, it improves the oral flora environment, thereby improving oral health.

[0004] The second technical problem to be solved by the present invention is to provide an application of cranberry extract in enhancing the growth promotion of oral non-pathogenic bacteria by an oral care composition containing inulin.

[0005] The third technical problem to be solved by the present invention is to provide an oral care composition comprising cranberry extract and inulin for use in enhancing the ability to inhibit bacterial adhesion on the oral cavity or tooth surface.

[0006] In order to solve the above-mentioned first technical problem, the invention adopts the following technical solution :

[0007] An oral care composition comprising cranberry extract and inulin, comprising:

[0008] 1) Cranberry extract,

[0009] 2) Inulin and

[0010] 3) an orally acceptable carrier,

[0011] in,

[0012] The mass proportion of the cranberry extract in the oral care composition is 0.001-0.6%.

[0013] The inulin accounts for 0.1-6% by weight in the oral care composition.

[0014] As an embodiment, the cranberry extract contains cranberry polyphenols.

[0015] As an embodiment, the mass proportion of the cranberry extract in the oral care composition is 0.004-0.5%.

[0016] As an embodiment, the mass proportion of the inulin in the oral care composition is 0.5-5%.

[0017] As an embodiment, the composition further comprises xylitol.

[0018] As an embodiment, the composition further comprises erythritol.

[0019] As an embodiment, the composition comprises xylitol and erythritol.

[0020] As an embodiment, the mass proportion of xylitol in the oral care composition is 0.1-5%.

[0021] As an embodiment, the mass proportion of the erythritol in the oral care composition is 0.1-5%.

[0022] In order to solve the above second technical problem, the present invention adopts the following technical solution :

[0023] The present invention provides application of cranberry extract in enhancing the growth promotion of oral non-pathogenic bacteria by an oral care composition containing inulin.

[0024] In order to solve the third technical problem mentioned above, the present invention adopts the following technical solution :

[0025] The present invention provides an application of an oral care composition comprising cranberry extract and inulin in improving the ability to inhibit bacterial adhesion on the oral cavity or tooth surface.

[0026] Any range described in the present invention includes the end value and any numerical value between the end values and any sub-range formed by the end value or any numerical value between the end values.

[0027] Unless otherwise specified, all raw materials in the present invention can be purchased commercially, and the equipment used in the present invention can adopt conventional equipment in the relevant field or refer to the existing technology in the relevant field.

[0028] Compared with the prior art, the present invention has the following beneficial effects:

[0029] The oral care composition of the present invention promotes the growth of non-pathogenic bacteria in the oral cavity, while having no promoting effect on, or even inhibiting, oral pathogenic bacteria, thereby effectively improving the oral flora environment and oral health. The composition of the present invention can also effectively inhibit bacterial adhesion to the oral cavity or tooth surfaces. DETAILED DESCRIPTION

[0030] In order to explain the present invention more clearly, the present invention is further described below in conjunction with preferred embodiments. Those skilled in the art should understand that the following specific description is illustrative rather than restrictive and should not be used to limit the scope of protection of the present invention.

[0031] Unless otherwise indicated, all percentages and ratios used herein are by weight of the total composition. Unless otherwise indicated, all percentages, ratios and amounts of ingredients mentioned herein are based on the actual amount of the ingredient and do not include solvents, fillers or other substances that may be combined with these ingredients in commercially available products.

[0032] The term "comprises / comprising" herein means that other steps and ingredients which do not affect the end result can be added.

[0033] The term "preferably" and its variants herein refer to embodiments of the present invention that can provide specific beneficial effects under specific circumstances. However, other embodiments may also be preferred under the same or other circumstances. In addition, the detailed description of one or more preferred embodiments does not mean that other embodiments are useless and is not intended to exclude other embodiments from the scope of the present invention.

[0034] If no specific conditions are specified in the examples of the present invention, the experiments were carried out according to conventional conditions or the conditions recommended by the manufacturer. If no manufacturer is specified for the reagents or instruments used, they are all conventional products that can be purchased commercially.

[0035] As one aspect of the present invention, an oral care composition comprising cranberry extract and inulin comprises:

[0036] 1) Cranberry extract,

[0037] 2) Inulin and

[0038] 3) an orally acceptable carrier,

[0039] in,

[0040] The mass proportion of the cranberry extract in the oral care composition is 0.001-0.6%.

[0041] The inulin accounts for 0.1-6% by weight in the oral care composition.

[0042] It is well known to those skilled in the art that inulin is commonly used as a prebiotic in the prior art and has the effect of promoting the growth of non-pathogenic oral bacteria. However, cranberry extract is commonly used as an anti-oral bacterial adhesion agent and there are no reports on its effect of promoting the growth of non-pathogenic oral bacteria. The present invention unexpectedly discovered that adding cranberry extract to an oral care composition containing inulin can effectively enhance the growth-promoting effect of the composition on non-pathogenic oral bacteria. Even more surprisingly, the oral care composition containing cranberry extract and inulin also significantly enhances its ability to inhibit the adhesion of pathogenic bacteria in the oral cavity (teeth).

[0043] Cranberry extract

[0044] Cranberry, also known as cranberry or cranberry, is an evergreen shrub belonging to the genus Vacinium macrocarpon in the Ericaceae family. It is one of the three major fruits in North America and is known as the "Ruby of North America." The main active ingredients in cranberry extract are polyphenols such as anthocyanins, proanthocyanidins, phenolic acids, and ellagic acid. According to existing technologies, cranberry polyphenols have the function of preventing bacterial adhesion in the mouth or on tooth surfaces. In addition, cranberry extract also contains flavonoids, flavonols, phenolic acids, ellagic acid, resveratrol, hyperoside, quercetin, and other active ingredients beneficial to the human body.

[0045] According to some embodiments of the present invention, the cranberry extract comprises cranberry polyphenols.

[0046] According to some embodiments of the present invention, the cranberry polyphenols comprise one or more combinations of anthocyanidins, proanthocyanidins, phenolic acids, and ellagic acid.

[0047] According to certain embodiments of the present invention, the mass proportion of the cranberry extract in the oral care composition is 0.001-0.6%, for example, but not limited to, 0.001-0.5%, 0.002-0.5%, 0.004-0.6%, 0.004-0.5%, 0.004-0.4%, 0.004-0.3%, 0.004-0.2%, 0.004-0.1%, 0.008-0.5%, 0.0 1-0.5%, 0.05-0.5%, 0.1-0.5%, 0.3-0.5%, 0.2-0.4%, 0.3-0.4%, 0.001-0.3%, 0.002-0.3%, 0.008-0.3%, 0.01-0.3%, 0.05-0.3%, 0.1-0.3%, 0.2-0.3%, 0.001-0.1%, 0.001-0.05%, 0.04-0.5%.

[0048] Inulin

[0049] Inulin is a natural fructan mixture composed of D-fructofuranose molecules linked by β-(2,1) glycosidic bonds, with each inulin molecule end linked by an α-(1,2) glycosidic bond to a glucose residue. Inulin is a soluble dietary fiber that is virtually indigestible by gastric acid and can only be utilized by beneficial microorganisms in the colon, thereby improving the intestinal flora and, consequently, the intestinal environment. Therefore, it is often used as a prebiotic in the prior art. Prior art also reports that inulin promotes the growth of non-pathogenic oral bacteria. The present invention unexpectedly discovered that an oral care composition combining inulin with cranberry extract can further enhance the growth-promoting effect of inulin on non-pathogenic oral bacteria, thereby improving the balance of oral flora and oral health.

[0050] According to certain embodiments of the present invention, the mass proportion of inulin in the oral care composition is 0.1-6%, for example, but not limited to 0.1-5%, 0.1-4%, 0.1-3%, 0.1-2%, 0.1-1%, 0.1-1%, 0.1-0.5%, 0.3-6%, 0.3-5%, 0.3-4%, 0.3-3%, 0.3-2%, 0.3-1%, 0.5-6%, 0.5-5%, 0.5-4%, 0.5-3%, 1-6%, 1-5%, 1-4%, 1-3%, 1-2%, 2-5%, 2-4%, 2-3%, 3-5%, 3-4%, 4-5%.

[0051] Xylitol

[0052] Xylitol is a naturally occurring five-carbon sugar alcohol with the chemical formula C5H 12 O5. Xylitol has significant application value in oral care due to its unique metabolic properties: xylitol is not fermented by oral bacteria to produce acid, thus helping to reduce plaque formation and the occurrence of tooth decay. Furthermore, xylitol's hygroscopicity and cooling properties can improve the taste and texture of oral care products.

[0053] The present invention unexpectedly discovered that when xylitol is further added to an oral care composition comprising cranberry extract and inulin, the oral non-pathogenic bacteria growth promoting effect and the anti-bacterial adhesion effect of the composition are effectively enhanced.

[0054] According to certain embodiments of the present invention, the mass proportion of inulin in the oral care composition is 0.1-5%, for example, but not limited to 0.1-4%, 0.1-3%, 0.1-2%, 0.1-1%, 0.1-1%, 0.1-0.5%, 0.3-5%, 0.3-4%, 0.3-3%, 0.3-2%, 0.3-1%, 0.5-5%, 0.5-4%, 0.5-3%, 1-5%, 1-4%, 1-3%, 1-2%, 2-5%, 2-4%, 2-3%, 3-5%, 3-4%, 4-5%.

[0055] erythritol

[0056] Erythritol is a natural four-carbon sugar alcohol with the chemical formula C4H 10 O4. Erythritol has significant advantages in the oral care field due to its unique physical and chemical properties: erythritol is not metabolized by oral microorganisms to produce acid, so it does not cause enamel demineralization or the formation of tooth decay; at the same time, it has an endothermic effect when dissolved, which can give oral care products a refreshing taste experience.

[0057] The present invention unexpectedly discovered that when erythritol is further added to an oral care composition comprising cranberry extract and inulin, the oral non-pathogenic bacteria growth promoting effect and anti-bacterial adhesion effect of the composition are effectively enhanced, especially when xylitol and erythritol are added to the composition at the same time, the oral non-pathogenic bacteria growth promoting effect and anti-bacterial adhesion effect of the composition can be further enhanced.

[0058] According to some embodiments of the present invention, the mass proportion of erythritol in the oral care composition is 0.1-5%, for example, but not limited to 0.1-4%, 0.1-3%, 0.1-2%, 0.1-1%, 0.1-1%, 0.1-0.5%, 0.3-5%, 0.3-4%, 0.3-3%, 0.3-2%, 0.3-1%, 0.5-5%, 0.5-4%, 0.5-3%, 1-5%, 1-4%, 1-3%, 1-2%, 2-5%, 2-4%, 2-3%, 3-5%, 3-4%, 4-5%.

[0059] Orally acceptable carrier

[0060] In the present invention, the "orally acceptable carrier" refers to any vehicle suitable for formulating the oral care composition disclosed in the present invention; when the orally acceptable carrier is retained in the mouth in the amount disclosed in the present invention without being swallowed for a period of time sufficient to allow effective contact with the tooth surface as required by the present invention, it is harmless to mammals; in general, the orally acceptable carrier will not be harmful even if it is swallowed unintentionally; suitable orally acceptable carriers include, for example, one or more of the following substances: water, thickeners, buffers, wetting agents, surfactants, abrasives, sweeteners, flavorings, visual aids (for example, pigments, dyes or mixtures thereof), anti-caries agents, antibacterial agents, whitening agents, desensitizing agents, vitamins, preservatives, enzymes and mixtures thereof, etc.

[0061] As another aspect of the present invention, the present invention provides use of a cranberry extract in enhancing the growth promotion of an oral care composition containing inulin on oral non-pathogenic bacteria.

[0062] As another aspect of the present invention, the present invention provides an application of an oral care composition comprising a cranberry extract and inulin in improving the ability to inhibit bacterial adhesion on the oral cavity or tooth surface.

[0063] 1. Evaluation method of the effect of oral bacteria growth

[0064] 1) Oral resident bacteria

[0065] Non-pathogenic bacteria (abbreviated as: non-pathogenic bacteria):

[0066] Streptococcus sanguis (abbreviated as S. sanguis), a suspected pathogen of periodontal disease, can antagonize it by producing hydrogen peroxide and sanguisin, and is the main beneficial bacteria of periodontitis.

[0067] Pathogenic bacteria (referred to as: pathogenic bacteria):

[0068] Streptococcus mutans (abbreviated as S.mutans), Streptococcus mutans, is one of the main components of dental plaque and the main pathogen of dental caries.

[0069] Porphyromonas gingivalis (abbreviated as P.gingivalis), a Gram-negative anaerobic cocci, is a non-fermenting saccharolytic bacillus and one of the important periodontal pathogens with extensive research and sufficient evidence.

[0070] 2) Culture medium preparation: 0.0005% hemin and 0.0001% menadione were added to Tryptic Soy Medium (TS medium, manufactured by Becton and Dickinson) to prepare the experimental culture medium.

[0071] 3) Preparation of bacterial suspension: Bacteria cultured in the above medium were inoculated at a concentration of 2% into a 2-fold concentration series of culture media to obtain bacterial suspension;

[0072] 4) Place 100 μL of sample in a 96-well plate, add 100 μL of bacterial solution, and incubate anaerobically at 37°C for approximately 20 hours;

[0073] 5) Shake the 96-well plate and measure the turbidity of the culture medium at a wavelength of 630 nm;

[0074] 6) The turbidity of the control group (sample addition amount is 0%) is used as the relative value of the bacterial growth effect. When the growth effect is greater than 1, growth is promoted, and the larger the value, the better the growth promotion effect; when the growth effect is less than 1, growth is inhibited.

[0075] 2. Evaluation method of anti-adhesion test

[0076] 1) Place the HAp sheet onto a 24-well plate, inject 2 ml of sample solution, and completely soak at 37°C for 1 hour.

[0077] 2) Remove the HAp and rinse with deionized water three times, two minutes each time;

[0078] 3) After washing, the HAp sheet was placed in a new 24-well plate and injected with 2 ml of 0.2 OD saliva mixed bacteria. The film was cultured at 37 °C.

[0079] 4) After 6 hours, replace the TS medium and continue culturing at 37°C for 18 hours.

[0080] 5) Take out the HAp and place it in a 14 ml centrifuge tube with 2 ml of 0.25% trypsin solution, and incubate at 37°C for 30 minutes.

[0081] 6) Remove the centrifuge tube and shake for 10 seconds. Transfer 250 μl of the solution to a 96-well plate and measure the OD value of the solution at 630 nm. Specific embodiments

[0083] Example 1, Comparative Examples 1-3

[0084] According to the formula in Table 1 below, oral care compositions of Example 1 and Comparative Examples 1-3 were prepared. The data in the table are all parts by weight, totaling 100 parts. In the present invention, the oral care compositions prepared according to the formula can be prepared by conventional mixing.

[0085] Table 1:

[0086]

[0087] After Example 1 and Comparative Examples 1-3 were prepared, the effects of the compositions on the growth of different oral bacteria were evaluated. The results are shown in Table 2.

[0088] Table 2:

[0089] Comparative Example 1 Comparative Example 2 Comparative Example 3 Example 1 S. sanguis 1 0.99 1.06 1.21 S. mutans 1 1.01 0.97 0.96 P.gingivalis 1 1 0.99 0.98

[0090] From Table 1 and Table 2 we can see that:

[0091] 1) Comparative Example 1 was used as a negative control. No cranberry extract or inulin was added to the formula. The effect on the growth of three oral bacteria was recorded as 1 unit.

[0092] 2) The formula of Comparative Example 2 added 0.001% cranberry extract. The results showed that the formula of Comparative Example 2 had essentially no growth promoting or inhibitory effect on the three oral bacteria.

[0093] 3) Comparative Example 3 added 0.1% inulin. The results showed that the formula of Comparative Example 3 had a slight growth-promoting effect on the non-pathogenic bacteria S. sanguis, but had a slight inhibitory effect on the other two oral pathogens;

[0094] 4) Example 1 simultaneously added 0.001% cranberry and 0.1% inulin. The results showed that compared with Comparative Examples 2 and 3, Example 1 significantly promoted the growth of the non-pathogenic oral bacteria S. sanguis, while having no significant effect on the growth of the other two oral pathogens.

[0095] In summary, when cranberry extract and inulin are added to the composition at the same time, the composition has the effect of synergistically promoting the growth of beneficial bacteria.

[0096] Comparative Examples 4-8

[0097] According to the formula in Table 3 below, oral care compositions of Comparative Examples 4-8 were prepared. The data in the table are all in parts by weight, and the total amount is 100 parts. In the present invention, the oral care compositions prepared according to the formula can be prepared by conventional mixing.

[0098] Table 3:

[0099]

[0100] After the preparation of the above comparative examples 4-8 was completed, the effects of the compositions on the bacterial growth of different oral bacteria were evaluated. The results are shown in Table 4.

[0101] Table 4:

[0102] Comparative Example 4 Comparative Example 5 Comparative Example 6 Comparative Example 7 Comparative Example 8 S. sanguis 1 0.98 1.01 0.99 1.01 S. mutans 0.99 0.97 1 0.97 0.98 P.gingivalis 1 0.99 0.96 1 0.99

[0103] From Table 3 and Table 4 we can see that:

[0104] 1) The composition of Comparative Example 4 was added with 0.004% by weight of cranberry extract;

[0105] 2) The composition of Comparative Example 5 was added with 0.01% by weight of cranberry extract;

[0106] 3) The composition of Comparative Example 6 was added with 0.1% by weight of cranberry extract;

[0107] 4) The composition of Comparative Example 7 was added with 0.5% by weight of cranberry extract;

[0108] 5) The composition of Comparative Example 8 was added with 0.6% by weight of cranberry extract;

[0109] From the results in Table 4, it can be seen that the effects of the composition formulas of Comparative Examples 4-8 on the bacterial growth of the three oral bacteria are not very different, that is, the cranberry extract does not show better growth inhibition or promotion effects on the three oral bacteria with increasing concentration.

[0110] Examples 2-6

[0111] Oral care compositions of Examples 2-6 were prepared according to the formulations in Table 5 below. All data in the table are in parts by weight, totaling 100 parts. For ease of comparison, the formulations of Example 1 and Comparative Example 3 are listed in Table 5. In the present invention, oral care compositions prepared according to the formulations are prepared by conventional mixing.

[0112] Table 5:

[0113]

[0114] After the preparation of Examples 2-6 was completed, the effects of the compositions on the bacterial growth of different oral bacteria were evaluated. The results are shown in Table 6.

[0115] Table 6:

[0116] Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Comparative Example 3 S. sanguis 1.21 1.26 1.42 1.51 1.69 1.52 1.06 S. mutans 0.96 0.99 1.01 0.98 1 0.96 0.97 P.gingivalis 0.98 1.01 0.98 0.99 0.96 0.97 0.99

[0117] As can be seen from Tables 5 and 6, Examples 1-6 and Comparative Example 3 all added 0.1% by mass of inulin, the difference being that the mass ratios of cranberries added were different, wherein:

[0118] 1) Example 1 added 0.001% cranberry extract;

[0119] 2) Example 2 added 0.004% cranberry extract;

[0120] 3) Example 3 added 0.01% cranberry extract;

[0121] 4) Example 4 added 0.1% cranberry extract;

[0122] 5) Example 5 added 0.5% cranberry extract

[0123] 6) In Example 6, 0.6% of cranberry extract was added.

[0124] From the bacterial growth results, it can be seen that there is no significant difference in the effects of Examples 1-6 on the growth of oral pathogenic bacteria. In contrast, the effect of the composition on the growth of oral non-pathogenic bacteria shows that the growth-promoting effect of the composition on oral non-pathogenic bacteria increases with the increase in the mass proportion of cranberry in the composition. However, when the concentration of cranberry extract reaches 0.6%, the bacterial growth effect of the composition decreases instead of increases.

[0125] Examples 7-12, Comparative Example 9

[0126] Oral care compositions of Examples 7-12 and Comparative Example 9 were prepared according to the formula in Table 7 below. The data in the table are all parts by weight, and the total is 100 parts. In the present invention, the oral care compositions prepared according to the formula can be prepared by conventional mixing.

[0127] Table 7:

[0128]

[0129] After the preparation of Examples 7-12 and Comparative Example 9, the effects of the compositions on the growth of different oral bacteria were evaluated. The results are shown in Table 8.

[0130] Table 8:

[0131]

[0132] From Tables 7 and 8 we can see that:

[0133] Examples 7-12 and Comparative Example 9 all added 5% by mass of inulin, the difference being that the mass ratios of cranberries added were different, wherein:

[0134] 1) Example 7 added 0.001% cranberry extract;

[0135] 2) Example 8 added 0.04% cranberry extract;

[0136] 3) Example 9 added 0.01% cranberry extract;

[0137] 4) Example 10 added 0.1% cranberry extract

[0138] 5) Example 11 added 0.5% cranberry extract;

[0139] 6) Example 12 added 0.6% cranberry extract;

[0140] From the bacterial growth results we can see that:

[0141] There is no significant difference in the effects of Examples 7-12 on the growth of oral pathogenic bacteria, and the trend of the effects on the growth of oral non-pathogenic bacteria is consistent with that of Examples 1-6. The oral care composition containing cranberry extract and inulin has an increasing growth-promoting effect on oral non-pathogenic bacteria as the mass proportion of cranberry in the composition increases. However, when the concentration of cranberry extract reaches 0.5% (for example, increasing from 0.5% to 0.6%), the bacterial growth effect of the composition does not increase further.

[0142] Comparative Examples 10-13

[0143] Oral care compositions of Comparative Examples 10-13 were prepared according to the formula in Table 9 below. The data in the table are all in parts by weight, and the total amount is 100 parts. In the present invention, the oral care compositions prepared according to the formula can be prepared by conventional mixing.

[0144] Table 9:

[0145] Comparative Example 10 Comparative Example 11 Comparative Example 12 Comparative Example 13 Cranberry extract / 0.1 / 0.1 Inulin 0.05 0.05 6 6 Deionized water Add to 100% Add to 100% Add to 100% Add to 100%

[0146] After the preparation of the above comparative examples 10-13 was completed, the effects of the compositions on the growth of oral beneficial bacteria were evaluated. The results are shown in Table 10.

[0147] Table 10:

[0148] Comparative Example 10 Comparative Example 11 Comparative Example 12 Comparative Example 13 S. sanguis 1 1.01 2.21 2.2

[0149] From Table 9 and Table 10 we can see that:

[0150] 1) The composition formulas of Comparative Example 10 and Comparative Example 13 did not contain cranberry extract;

[0151] 2) Comparative Example 11 is based on Comparative Example 10, with 0.1% by weight of cranberry extract added;

[0152] 3) Comparative Example 12 is based on Comparative Example 13, with 0.1% by mass of cranberry extract added.

[0153] From the results:

[0154] 1) Compared with Comparative Example 10, Comparative Example 11 did not show a better effect of promoting the growth of oral non-pathogenic bacteria;

[0155] 2) Compared with Comparative Example 13, Comparative Example 12 did not show a better effect of promoting the growth of oral non-pathogenic bacteria;

[0156] Combining the data from Comparative Example 3 in Tables 1 and 2, Example 3 in Tables 5 and 6, and Example 9 and Comparative Example 9 in Tables 7 and 8, it can be concluded that when the mass percentage of inulin in the composition is less than or equal to 0.05% and greater than or equal to 6%, cranberry extract does not enhance the efficacy of inulin in promoting the growth of non-pathogenic oral bacteria. In other words, when the mass percentage of inulin in the composition is between 0.1% and 5%, cranberry extract effectively enhances the growth-promoting effect of inulin on non-pathogenic oral bacteria.

[0157] Examples 13-14, Comparative Example 14

[0158] According to the formula in Table 11 below, oral care compositions of Examples 13-14 and Comparative Example 14 were prepared. The data in the table are all in parts by weight, totaling 100 parts. In the present invention, the oral care compositions prepared according to the formula can be prepared by conventional mixing.

[0159] Table 11:

[0160] Example 13 Comparative Example 14 Example 15 Cranberry extract 0.004 0.004 0.004 Inulin 2 / 1 Xylitol / 2 1 Deionized water Add to 100% Add to 100% Add to 100%

[0161] After the preparation of Examples 13-14 and Comparative Example 14, the effects of the compositions on the bacterial growth of different oral bacteria were evaluated. The results are shown in Table 12.

[0162] Table 12:

[0163] Example 13 Comparative Example 14 Example 14 S. sanguis 1.62 0.9 1.59 S. mutans 0.94 0.72 0.76 P.gingivalis 0.95 0.88 0.84

[0164] From Table 11 and Table 12, we can see that:

[0165] 1) Example 13 added 0.004% by weight of cranberry extract and 2% by weight of inulin;

[0166] 2) Comparative Example 14 added 0.004% by weight of cranberry extract and 2% by weight of xylitol;

[0167] 3) In Example 14, 0.004% by mass of cranberry extract, 1% by mass of inulin, and 1% by mass of xylitol were added simultaneously.

[0168] From the results:

[0169] 1) Example 13 promoted the growth of non-pathogenic oral bacteria, but had no significant growth inhibitory effect on oral pathogenic bacteria;

[0170] 2) The formulation in Comparative Example 14 inhibited the growth of three bacteria to varying degrees;

[0171] 3) The growth promotion effect of Example 14 on oral non-pathogenic bacteria is basically the same as that of Example 13, but the inhibitory effect on oral pathogenic bacteria has been significantly improved compared with Example 13.

[0172] From the above, it can be seen that the oral care composition containing cranberry extract and inulin has a significant effect of promoting the growth of non-pathogenic oral bacteria; the oral care composition containing cranberry extract, inulin and xylitol has a synergistic effect of significantly promoting the growth of non-pathogenic oral bacteria and significantly inhibiting the growth of oral pathogenic bacteria.

[0173] Example 15, Comparative Example 15

[0174] Oral care compositions of Example 15 and Comparative Example 15 were prepared according to the formulations in Table 13 below. All data in the table are in parts by weight, totaling 100 parts. For ease of comparison, Example 13 is included in Table 13. In the present invention, oral care compositions prepared according to the formulations are prepared by conventional mixing.

[0175] Table 13:

[0176] Example 13 Comparative Example 15 Example 15 Cranberry extract 0.004 0.004 0.004 Inulin 2 / 1 erythritol / 2 1 Deionized water Add to 100% Add to 100% Add to 100%

[0177] After Example 15 and Comparative Example 15 were prepared, the effects of the compositions on the bacterial growth of different oral bacteria were evaluated. The results are shown in Table 14.

[0178] Table 14:

[0179] Example 13 Comparative Example 15 Example 15 S. sanguis 1.62 0.95 1.65 S. mutans 0.94 0.92 0.76 P.gingivalis 0.95 0.88 0.81

[0180] From Table 13 and Table 14 we can see that:

[0181] 1) Example 13 added 0.004% by weight of cranberry extract and 2% by weight of inulin;

[0182] 2) Comparative Example 15 added 0.004% by mass of cranberry extract and 2% by mass of erythritol;

[0183] 3) In Example 15, 0.004% by mass of cranberry extract, 1% by mass of inulin, and 1% by mass of erythritol were added simultaneously.

[0184] From the results:

[0185] 1) Example 13 significantly promoted the growth of non-pathogenic oral bacteria, but had no significant growth inhibitory effect on oral pathogenic bacteria;

[0186] 2) The formulation of Comparative Example 15 had a slight inhibitory effect on all three bacteria;

[0187] 3) The growth promotion effect of Example 15 on oral non-pathogenic bacteria is basically the same as that of Comparative Example 14, and the inhibitory effect on oral pathogenic bacteria has been significantly improved compared with Examples 13 and 15.

[0188] From the above, it can be seen that the oral care composition containing cranberry extract and inulin has a significant effect of promoting the growth of non-pathogenic oral bacteria; the oral care composition containing cranberry extract, inulin and erythritol has a synergistic effect of significantly promoting the growth of non-pathogenic oral bacteria and significantly inhibiting the growth of oral pathogenic bacteria.

[0189] Examples 16-18, Comparative Examples 16-17

[0190] Oral care compositions of Examples 16-18 and Comparative Examples 16-17 were prepared according to the formulations in Table 15 below. All data in the table are in parts by weight, totaling 100 parts. For ease of comparison, Comparative Example 1, Comparative Example 4, Comparative Example 3, and Example 2 are listed in Table 15. In the present invention, oral care compositions prepared according to the formulations are prepared by conventional mixing.

[0191] Table 15:

[0192]

[0193] After the above comparative example and example compositions were prepared, the anti-bacterial adhesion effects of different compositions were evaluated according to the above anti-adhesion test evaluation method. The results are shown in Table 16.

[0194] Table 16:

[0195]

[0196] From Table 15 and Table 16 we can see that:

[0197] 1) Comparative Example 16 added 0.5% by weight of xylitol;

[0198] 2) Comparative Example 17 added 0.5% by weight of erythritol;

[0199] 3) Example 16 simultaneously added 0.004% by weight of cranberry extract, 0.1% by weight of inulin, and 0.5% by weight of xylitol;

[0200] 4) Example 17 simultaneously added 0.004% by weight of cranberry extract, 0.1% by weight of inulin, and 0.5% by weight of erythritol;

[0201] 5) Example 18 simultaneously added 0.004% by weight of cranberry extract, 0.1% by weight of inulin, 0.25% by weight of xylitol, and 0.25% by weight of erythritol;

[0202] From the results:

[0203] 1) The OD value of Comparative Example 1 without adding any ingredients was 0.33;

[0204] 2) The OD value of Comparative Example 4, in which 0.004 wt.% cranberry extract was added, was 0.254, which showed an antibacterial adhesion effect compared to Comparative Example 1;

[0205] 3) The OD value of Comparative Example 3, in which 0.1 wt.% inulin was added, was comparable to that of Comparative Example 1, indicating no antibacterial adhesion effect.

[0206] 4) The OD value of Comparative Example 16, in which 0.5 wt.% xylitol was added, was comparable to that of Comparative Example 1, indicating no antibacterial adhesion effect.

[0207] 5) The OD value of Comparative Example 17, in which 0.5 wt.% erythritol was added, was comparable to that of Comparative Example 1, indicating no antibacterial adhesion effect.

[0208] 6) The OD value of Example 2, in which 0.004 wt.% cranberry extract and 0.1 wt.% inulin were added, was 0.214, showing a significantly improved antibacterial adhesion effect compared to Comparative Examples 4 and 3.

[0209] 7) The OD value of Example 16, in which 0.004 wt.% cranberry extract, 0.1 wt.% inulin, and 0.5 wt.% xylitol were added, was 0.187, which further improved the antibacterial adhesion effect compared to Example 2.

[0210] 8) The OD value of Example 17, in which 0.004 wt.% cranberry extract, 0.1 wt.% inulin, and 0.5 wt.% erythritol were added, was 0.179, which further improved the antibacterial adhesion effect compared to Example 2.

[0211] 9) The OD value of Example 18, to which 0.004 wt.% cranberry extract, 0.1 wt.% inulin, 0.25 wt.% xylitol, and 0.25 wt.% erythritol were added, was 0.160, showing an improved antibacterial adhesion effect compared to Examples 16 and 17.

[0212] In summary, the oral care composition containing cranberry extract and inulin has greatly improved the ability to inhibit the adhesion of pathogenic bacteria in the oral cavity (teeth); in particular, the oral care composition containing cranberry extract, inulin, xylitol and erythritol has a synergistic effect in promoting anti-bacterial adhesion.

[0213] In the description of this specification, reference to the terms "one embodiment," "some embodiments," "example," "specific example," or "some examples" means that the specific features, materials, or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the embodiment of this specification. In this specification, the exemplary expressions of the above terms do not necessarily refer to the same embodiment or example.

[0214] In addition, those skilled in the art may combine and combine the different embodiments or examples described in this specification, as well as the features of the different embodiments or examples, unless they are mutually inconsistent. The above description is merely an embodiment of the embodiment of this specification and is not intended to limit the embodiment of this specification. For those skilled in the art, various changes and modifications may be made to the embodiment of this specification. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the embodiment of this specification shall be included within the scope of the claims of the embodiment of this specification.

Claims

1. An oral care composition comprising cranberry extract and inulin, characterized in that include: 1) Cranberry extract, 2) Inulin and 3) an orally acceptable carrier, in, The mass proportion of the cranberry extract in the oral care composition is 0.001-0.6%. The inulin accounts for 0.1-6% by weight in the oral care composition.

2. The oral care composition comprising cranberry extract and inulin according to claim 1, characterized in that: The cranberry extract contains cranberry polyphenols.

3. The oral care composition comprising cranberry extract and inulin according to claim 1, characterized in that: The mass proportion of the cranberry extract in the oral care composition is 0.004-0.5%.

4. The oral care composition comprising cranberry extract and inulin according to claim 1, characterized in that: The mass proportion of the inulin in the oral care composition is 0.5-5%.

5. The oral care composition comprising cranberry extract and inulin according to claim 1, characterized in that: The composition also includes xylitol.

6. The oral care composition comprising cranberry extract and inulin according to claim 1, characterized in that: The composition also includes erythritol.

7. The oral care composition comprising cranberry extract and inulin according to claim 1, characterized in that: The composition comprises xylitol and erythritol.

8. The oral care composition comprising cranberry extract and inulin according to claim 1, characterized in that: The mass proportion of xylitol in the oral care composition is 0.1-5%.

9. The oral care composition comprising cranberry extract and inulin according to claim 1, characterized in that: The mass proportion of the erythritol in the oral care composition is 0.1-5%.

10. Use of cranberry extract in enhancing the growth promotion of oral non-pathogenic bacteria in an oral care composition containing inulin.

11. Use of an oral care composition comprising cranberry extract and inulin for enhancing the ability to inhibit bacterial adhesion on oral or tooth surfaces.

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