Anti-hair loss and hair strengthening composition as well as preparation method and application thereof

Abstract

The invention provides an anti-hair loss and hair strengthening composition as well as a preparation method and application thereof, and belongs to the technical field of cosmetics. The hair loss preventing and hair strengthening composition is prepared from the following components in percentage by weight: 3 to 6 percent of a hair conditioner, 0.1 to 1 percent of a humectant, 0.5 to 1.0 percent of a preservative, 0.01 to 0.06 percent of a coloring agent, 0.2 to 0.8 percent of a suspending agent and the balance of water. The plant extracts are used as a research basis, the types of the plant extracts are reasonably selected, the mass ratio of the plant extracts is reasonably controlled, and the plant extracts are matched with the suspending agent for use, so that the obtained composition is low in irritation and high in safety and has good hair loss preventing and hair strengthening effects.

Description

Technical Field

[0001] The present invention belongs to the technical field of cosmetics, and in particular relates to an anti-hair loss and hair solidifying composition, a preparation method and application thereof. Background Art

[0002] With the accelerating pace of life and increasing workload, hair loss disorders such as androgenic alopecia (seborrheic alopecia) and alopecia areata are on the rise, with a younger prevalence. This type of hair loss affects both appearance and aesthetics, placing significant stress and psychological burden on patients, which in turn severely impacts their quality of life. Minoxidil and finasteride are commonly used Western medications for hair loss treatment, but both are prone to relapse after discontinuation and can also cause side effects such as skin irritation, scalp itching, hirsutism on the face and hands, dizziness, anorexia, and tachycardia. Furthermore, while hair transplantation has some therapeutic benefits, its clinical application is limited by the surgical trauma, high cost, and the need for multiple surgeries. Non-drug treatments such as acupuncture are also less effective. In recent years, there have been increasing reports on the use of plant extracts, including those from traditional Chinese medicine, to treat hair loss. These plant-derived ingredients are relatively safe, effective, and easy to use. Animal studies have shown that astragalus, Polygonum multiflorum, Ligustrum lucidum, and Cuscuta australis, used alone or in combination, can stimulate the growth of hair follicles in cultured mice, increase follicle number, and accelerate hair regeneration. Clinical studies have also found that anti-hair loss and hair growth solutions composed of Chinese herbs such as Platycladus orientalis leaves, Polygonum multiflorum, Ligustrum lucidum fruit, Ligustrum lucidum rhizome, and Cuscuta australis can significantly promote hair growth in patients with hair loss. Therefore, this article summarizes recent research findings on the role of plant extracts in hair growth and development, both domestically and internationally, and outlines the mechanisms by which plant extracts regulate hair follicle cell signaling pathways, in order to provide a theoretical basis for the mechanisms of action of plant extracts in hair growth and development and for the development of clinical hair loss treatments.

[0003] Dihydrotestosterone (DHT), a metabolite of testosterone, plays a key role in androgenic alopecia. Patients with androgenic alopecia have higher levels of DHT in the scalp and serum, as well as higher levels and activity of 5α-reductase, than those in healthy individuals. This increases the ability of hair follicles to convert testosterone to DHT. Because 5α-reductase in hair follicles mediates the conversion of testosterone to DHT, the use of 5α-reductase inhibitors such as finasteride can prolong the growth phase of hair follicles, prevent hair loss, and promote hair growth. Experiments in mice have shown that extracts from Panax ginseng (CA Meyer) and alcohol extracts from Ligustrum lucidum (Ait.) can also inhibit 5α-reductase activity, reduce DHT levels in skin tissue, and promote hair growth. In addition, plant extracts such as fig (Ficus carica Linn.), rosemary (Rosmarinus officinalis), Ganoderma lucidum (Ganoderma lucidum), Polygonum multiflori (Polygonum multiflori), Cacumen platycladi and Cynomorium songaricum also have inhibitory effects on 5α-reductase, and therefore have great development prospects in preventing hair loss and promoting hair growth.

[0004] For example, Chinese patent CN110025775A discloses a mild hair-fixing anti-hair loss and hair-promoting essence and a preparation method thereof. The mild hair-fixing anti-hair loss and hair-promoting essence comprises the following raw materials: water, ethanol, Polygonum multiflorum extract, glycerin, Angelica sinensis extract, aloe extract, ginseng extract, tea polyphenols, corn gluten amino acids, vitamin H, folic acid, vitamin B3, pantothenic acid, vitamin B6, vitamin B2, vitamin B1, yeast polypeptides, cysteine, sodium benzoate, potassium sorbate, citric acid, linoleic acid, xylanase, and hydra extract. This invention uses Polygonum multiflorum extract, Angelica sinensis extract, Aloe vera extract, and Ginseng extract as traditional Chinese medicine ingredients in a shampoo essence. Licorice root nourishes blood and promotes blood circulation, unblocking the meridians; Ginseng dispels wind and dampness, promotes blood circulation, opens the pores, and promotes hair growth; Polygonum multiflorum promotes local blood circulation and nourishes hair; Angelica sinensis replenishes Qi and raises Yang, strengthens Qi and strengthens the exterior; and Aloe vera promotes scalp blood circulation and metabolism, replenishes nutrients for the hair papilla, promotes the recovery of damaged and infected scalps, reduces oil leakage from oily hair, and promotes new hair regeneration. However, research has found that adding only these traditional Chinese medicine components does not meet the required hair loss prevention and hair growth rates, requiring the addition of linoleic acid, xylanase, and hydra extract to achieve a synergistic effect.

[0005] Another example is Chinese patent CN118662418A, which discloses a natural plant extract composition for preventing and strengthening hair loss. Through the synergistic effects of seven plant extracts (Hypericum perforatum extract, Guaiacum officinale extract, Mangosteen fruit peel extract, Mango leaf extract, Polygonum multiflorum extract, Platycladus orientalis leaf extract, and Zingiber officinale extract) in a reasonable ratio, the composition effectively inhibits 5α-reductase, a key microorganism that causes seborrheic alopecia, such as Malassezia furfur, Propionibacterium acnes, and Staphylococcus aureus, thereby reducing the probability of seborrheic alopecia. The beneficial ingredients in the seven plant extracts promote blood circulation and regulate scalp oil secretion, promoting hair growth and improving the scalp environment. In addition, mango leaf extract and Polygonum multiflorum extract are rich in vitamins and nutrients, nourishing hair, enhancing hair elasticity and luster, and reducing hair breakage and shedding. However, the patent does not study the hair strengthening effect of the composition, and therefore it is impossible to confirm the hair strengthening effect of the natural plant extract composition.

[0006] In addition, the existing document "Analysis and Development of Anti-Hair Loss Shampoo Formula Ingredients, Perfect (Guangdong) Daily Necessities Co., Ltd., Lin Xiaofeng, Zhongshan, Guangdong, 528400" discloses that raw Platycladus orientalis leaves, Polygonum multiflorum, raw sun-dried ginseng, soapberry, ginger, Eclipta prostrata, Herba Polygonati Scutellariae, and Ligustrum lucidum fruits all have the effect of being used for hair care in traditional Chinese medicine. Raw Platycladus orientalis leaves are often used to treat problems such as dandruff and itchy scalp. Polygonum multiflorum has the effect of nourishing the liver and kidneys and blackening hair, and can enhance the elasticity and glossiness of hair. Raw sun-dried ginseng can moisturize hair, increase the softness and glossiness of hair, and help improve the problem of dry hair. Soapberry can effectively remove grease and dirt from hair, and also has anti-inflammatory and antipruritic effects. Ginger can promote blood circulation in the scalp, enhance the nutritional supply of hair, and help prevent hair loss and dry hair. Eclipta prostrata has the effect of nourishing hair and repairing hair scales, and is often used to treat problems such as dry and frizzy hair. Herba Ligustri Lucidi (Lavender's Ligustrum lucidum) has heat-clearing and detoxifying properties, dispelling wind and relieving itching, and can improve dandruff and itchy scalp. Ligustrum lucidum (Ligustrum lucidum) nourishes the liver and kidneys, darkens hair, and increases elasticity and luster, helping to prevent dryness and hair loss. The addition of these ingredients can enhance the hair-care benefits of shampoo and improve product quality, making them suitable ingredients for shampoo formulations.

[0007] In addition, Chinese herbal plant extracts have the advantages of easy access, simple production process, and low price. They are also compatible with many cosmetic raw materials. These plant extracts all have antioxidant and / or anti-inflammatory and antibacterial effects. For example, extracts from rosemary, ganoderma lucidum, ginseng, privet fruit, cynomorium songaricum, fig, polygonum multiflorum, and arborvitae can promote hair growth by inhibiting 5α-reductase, and have become a leading source of drug research and development for anti-hair loss and hair growth preparations. Existing results have demonstrated that a variety of plant extracts or their active ingredients have significant anti-hair loss and hair growth efficacy with minimal side effects. At the same time, compound Chinese herbal anti-hair loss cosmetics such as hair nourishing essences, Chinese herbal hair growth solutions, anti-hair loss and hair growth solutions, and Chinese herbal hair growth solutions, which are made by combining extracts from various plants such as ginseng, angelica, and polygonum multiflorum, have also been recognized for their efficacy and have good development prospects.

[0008] For example, the prior patent CN118121523A discloses a hair growth and blackening effect essence composition and its preparation method and application. The essence composition comprises the following components by weight percentage: 2-5% plant extract; 0.2-0.8% moisturizer; 0.4-1.0% conditioner; 0.5-0.8% preservative; 0.2-0.4% antioxidant and the balance water; the application uses a mixture of rice bran extract, ginger root extract, ginseng root extract, kelp extract, grapefruit seed extract and Centella asiatica extract. The invention uses a plant extract compound and controls their mass ratio to be 1-2:1-2:1-2:1-2:0.5-0.9:0.2-0.5. By controlling the specific components and mass ratios of the plant extract, humectant, and conditioner, the invention enables the essence composition to have better moisturizing, hair growth, and hair darkening effects. In terms of moisturizing, it can lock in moisture and achieve deeper moisturizing; in terms of hair growth, it can moisturize and repair hair follicles and stimulate hair follicle growth; in terms of hair darkening, it can effectively activate tyrosinase activity and promote melanin production. However, the patent also does not mention the hair solidification effect.

[0009] Although the existing technology discloses that a variety of plant extracts have the effects of preventing hair loss, relieving itching, nourishing, and anti-inflammatory, some studies lack quality control, lack statutory toxicological safety evaluation, lack accurate evaluation of the components of plant extracts, and there is no research on related hair-fixing effects. Therefore, it is necessary to develop a hair care composition with high safety and good hair loss prevention and hair-fixing effects to provide a more complete and accurate basis for the effects of plant extracts in hair loss and hair fixation. Summary of the Invention

[0010] Based on the deficiencies in the prior art, the present invention takes plant extracts as the research basis, rationally selects the types of plant extracts, rationally controls the mass ratio between the plant extracts, and uses them in combination with hair conditioners to make the obtained composition have low irritation, high safety, and excellent anti-hair loss and hair strengthening effects.

[0011] In order to achieve the above object, the present invention adopts the following technical solutions:

[0012] The invention discloses a hair loss prevention and hair solidification composition, which comprises the following components by weight percentage: 3-6% hair conditioner, 0.1-1% moisturizer, 0.5-1.0% preservative, 0.01-0.06% colorant, 0.2-0.8% suspending agent and the balance water.

[0013] Preferably, the anti-hair loss and hair solidifying composition comprises the following components by weight percentage: 4-5% hair conditioner, 0.3-0.8% moisturizer, 0.6-0.8% preservative, 0.03-0.05% colorant, 0.3-0.6% suspending agent and the balance water.

[0014] As some preferred embodiments, the hair conditioner has a weight percentage of 3%, 4%, 5% or 6%.

[0015] As some preferred embodiments, the moisturizing agent is present in an amount of 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.7%, 0.8%, 0.9% or 1.0% by weight.

[0016] As some preferred embodiments, the preservative has a weight percentage of 0.5%, 0.6%, 0.7%, 0.8%, 0.9% or 1.0%.

[0017] As some preferred embodiments, the colorant has a weight percentage of 0.01%, 0.02%, 0.03%, 0.04%, 0.05% or 0.06%.

[0018] As some preferred embodiments, the suspending agent has a weight percentage of 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.7% or 0.8%.

[0019] The hair conditioner includes hair conditioner 1;

[0020] The hair conditioner 1 is one or more of ginger (Zingiber officinale) root extract, ginseng (Panax ginseng) root extract, kelp (Laminaria japonica) extract, pomelo (CITRUS GRANDIS) seed extract, Centella asiatica (CENTELLA ASIATICA) extract and rice bran (ORYZASATIVA) extract;

[0021] Preferably, the hair conditioner 1 is a mixture of ginger (Zingiber officinale) root extract, ginseng (PANAX GINSENG) root extract, kelp (LAMINARIA JAPONICA) extract, grapefruit (CITRUS GRANDIS) seed extract, Centella asiatica (CENTELLA ASIATICA) extract and rice bran (ORYZA SATIVA) extract.

[0022] More preferably, the mass ratio of the ginger (ZINGIBER OFFICINALE) root extract, ginseng (PANAX GINSENG) root extract, kelp (LAMINARIA JAPONICA) extract, pomelo (CITRUS GRANDIS) seed extract, Centella asiatica (CENTELLA ASIATICA) extract, and rice (ORYZA SATIVA) bran extract is 4-6:4-6:4-6:3-5:0.1-0.5:0.000001.

[0023] Further preferably, the mass ratio of the ginger (ZINGIBER OFFICINALE) root extract, ginseng (PANAXGINSENG) root extract, kelp (LAMINARIA JAPONICA) extract, pomelo (CITRUS GRANDIS) seed extract, Centella asiatica (CENTELLA ASIATICA) extract, and rice (ORYZA SATIVA) bran extract is 5:5:5:3.8:0.25:0.000001.

[0024] More preferably, the hair conditioner 1 is purchased from Guangzhou Huayue Medical Aesthetics Research Institute with the trade name HYFTGF003.

[0025] The hair conditioner further comprises hair conditioner 2;

[0026] The hair conditioning agent 2 is Thuja orientalis leaf extract.

[0027] Preferably, the hair conditioner 2 is purchased from Guangzhou Jianeng Biotechnology Co., Ltd.

[0028] The hair conditioner further comprises hair conditioner 3;

[0029] The hair conditioning agent 3 is pyridoxine hydrochloride.

[0030] Preferably, the hair conditioner 2 is purchased from DSM Nutritional Products Ltd. under the trade name Pyridoxine Hydrochloride.

[0031] As some preferred embodiments, the mass ratio of the hair conditioner 1, hair conditioner 2 and hair conditioner 3 is 3-5:0.2-0.6:0.1-0.5;

[0032] As some more preferred embodiments, the mass ratio of hair conditioner 1, hair conditioner 2 and hair conditioner 3 is 3-4:0.3-0.4:0.2-0.4;

[0033] As some further preferred embodiments, the mass ratio of hair conditioner 1, hair conditioner 2 and hair conditioner 3 is 3.5:0.4:0.25.

[0034] Among the above components:

[0035] Ginger (ZINGIBER OFFICINALE) root extract: can inhibit the activity of Malassezia, reduce scalp inflammation and regulate sebaceous gland secretion, thereby improving the scalp environment; has strong antioxidant properties, helps protect hair from free radical damage, enhances hair gloss and improves hair's resistance to breakage; its active ingredients can stimulate the scalp, promote blood circulation, and help improve the nutrient supply to hair follicles.

[0036] Ginseng (PANAX GINSENG) root extract: can increase the expansion of capillaries in the head, thereby providing more nutrition to the hair and enhancing its toughness; helps improve blood circulation in the scalp, promotes the health of hair follicles, and thus reduces hair loss and breakage; by promoting the health of hair follicles, ginseng root extract can enhance the health of hair roots and reduce hair problems caused by malnutrition or poor blood circulation.

[0037] Kelp (LAMINARIA JAPONICA) extract: has antioxidant and anti-aging effects; can inhibit the generation of oxygen free radicals, thereby slowing down the aging process of skin and hair, maintaining the health of scalp and hair, delaying scalp aging, and reducing fine lines and sagging.

[0038] Citrus Grandis seed extract: Rich in B vitamins, protein and other trace elements, it can provide sufficient nourishment for hair, prevent hair loss and promote healthy hair growth; its nutrients have anti-inflammatory and qi-regulating effects, can stimulate hair follicles and accelerate hair growth; it has a strong effect on eliminating free radicals and has anti-aging and antioxidant effects.

[0039] Centella Asiatica Extract: Rich in antioxidants, it can reduce hair damage, increase elasticity and prevent breakage; it has a soothing and calming effect, can reduce hair loss, while soothing the scalp and building a strong scalp barrier; it can promote cell vitality and help hair growth.

[0040] Rice (Oryza Sativa) Bran Extract: This extract improves skin's softness by promoting the production of cholesterol and ceramides, increasing sebum levels. It also possesses antioxidant properties and can activate glutathione reductase, thus aiding in anti-aging efforts. These properties make rice (Oryza Sativa) bran extract a promising addition to shampoo products, improving scalp and hair health and reducing hair loss and thinning.

[0041] Thuja orientalis leaf extract: can inhibit scalp oil secretion and reduce hair loss; has antibacterial, moisturizing and astringent effects, and can help the skin lock in moisture; in anti-hair loss shampoo, Thuja orientalis leaf extract is one of the core ingredients, which can inhibit the production of DHT (dihydrotestosterone), thereby reducing hair loss problems, and is especially suitable for people with androgenic alopecia.

[0042] Pyridoxine hydrochloride: can help control oil secretion and make hair more fluffy; contribute to hair health, reduce hair loss, and strengthen hair roots.

[0043] During the implementation of the present invention, the types of plant extracts are controlled, that is, a mixture of ginger (Zingiber officinale) root extract, ginseng (Panax ginseng) root extract, kelp (Laminaria japonica) extract, grapefruit (Citrus grandis) seed extract, Centella asiatica (Centella asiatica) extract, and rice (Oryza sativa) bran extract is used as a hair conditioner. By controlling the mass ratio of the plant extracts to 4-6:4-6:4-6:3-5:0.1-0.5:0.000001, the antioxidant effect of the composition is significantly improved, and the hair follicle health can be better promoted to achieve the effect of hair strengthening. Thuja orientalis leaf extract can better inhibit the production of DHT (dihydrotestosterone), thereby reducing hair loss problems; pyridoxine hydrochloride can further reduce hair loss and strengthen hair roots.

[0044] During the implementation of the present invention, the hair loss prevention and hair strengthening effect of the composition can be significantly improved by controlling the mass ratio of hair conditioner 1, hair conditioner 2 and hair conditioner 3 to 3-5:0.2-0.6:0.1-0.5.

[0045] The moisturizing agent is selected from 1,2-pentanediol.

[0046] Preferably, the moisturizing agent is purchased from Guangdong Dimei Biotechnology Co., Ltd. and its trade name is Antimicro 512.

[0047] The preservative is selected from a mixture of phenoxyethanol and ethylhexylglycerin, with a mass ratio of phenoxyethanol and ethylhexylglycerin being 9:1.

[0048] Preferably, the preservative is purchased from Guangdong Dimei New Material Technology Co., Ltd. and its trade name is Antimicro9010.

[0049] The coloring agent is caramel color.

[0050] Preferably, the colorant is purchased from Shandong Aipu Food Industry Co., Ltd. and its trade name is caramel color.

[0051] The above suspending agent is a mixture of microcrystalline cellulose and glycerol.

[0052] Preferably, the suspending agent is a mixture of glycerol and microcrystalline cellulose in a ratio of 10:1.

[0053] More preferably, the suspending agent is purchased from Vincen Biotech (Guangzhou) Co., Ltd. with the trade name VINCEBIOHPC851.

[0054] As some preferred embodiments, the anti-hair loss and hair solidifying composition comprises the following components by weight percentage:

[0055]

[0056] As a further preferred embodiment, the anti-hair loss and hair solidifying composition comprises the following components by weight percentage:

[0057]

[0058]

[0059] As a further preferred embodiment, the anti-hair loss and hair solidifying composition comprises the following components by weight percentage:

[0060]

[0061] As a preferred embodiment, the anti-hair loss and hair solidifying composition comprises the following components by weight percentage:

[0062]

[0063]

[0064] As some preferred embodiments, the anti-hair loss and hair solidifying composition comprises the following components by weight percentage:

[0065]

[0066] As a preferred embodiment, the anti-hair loss and hair solidifying composition comprises the following components by weight percentage:

[0067]

[0068]

[0069] The present invention also provides a method for preparing the above-mentioned anti-hair loss and hair solidifying composition, comprising the following steps:

[0070] Heat water to 60-70°C, then add hair conditioner 1 and hair conditioner 2, and homogenize for 1-5 minutes; cool to below 45°C, add the remaining raw materials, continue stirring for 20-40 minutes, stir evenly until no solid particles are left, and cool to obtain the composition.

[0071] The present invention also provides a method for preparing the above-mentioned anti-hair loss and hair solidifying composition, comprising the following steps:

[0072] Heat water to 60-70°C, then add ginger root extract, ginseng root extract, kelp extract, grapefruit seed extract, Centella asiatica extract, rice bran extract and Platycladus orientalis leaf extract, and homogenize for 1-5 minutes; cool to below 45°C, add the remaining raw materials, continue stirring for 20-40 minutes, stir evenly until no solid particles are left, and cool to obtain the composition.

[0073] The present application also provides the use of the above-mentioned anti-hair loss and hair solidification composition in the preparation of an anti-hair loss and hair solidification product;

[0074] The anti-hair loss and hair strengthening product is an anti-hair loss and hair strengthening shampoo product or hair care product, and the shampoo product or hair care product includes but is not limited to shampoo, shampoo, conditioner, and hair care essence.

[0075] A hair loss prevention and hair strengthening shampoo product comprises the above-mentioned hair loss prevention and hair strengthening composition and auxiliary materials.

[0076] Furthermore, an anti-hair loss and hair strengthening shampoo product comprises the above-mentioned anti-hair loss and hair strengthening composition and auxiliary materials used for preparing shampoo.

[0077] Furthermore, an anti-hair loss and hair strengthening shampoo product comprises the above-mentioned anti-hair loss and hair strengthening composition and auxiliary materials used for preparing shampoo.

[0078] A hair loss prevention and hair strengthening product comprises the above-mentioned hair loss prevention and hair strengthening composition and auxiliary materials.

[0079] Furthermore, an anti-hair loss and hair strengthening product comprises the above-mentioned anti-hair loss and hair strengthening composition and auxiliary materials used for preparing hair conditioner.

[0080] Furthermore, an anti-hair loss and hair strengthening product comprises the above-mentioned anti-hair loss and hair strengthening composition and auxiliary materials used for preparing hair care essence.

[0081] The auxiliary materials are selected from one or more of hair conditioners, thickeners, emulsifiers, moisturizers and emollients.

[0082] Compared with the prior art, the present invention has the following beneficial effects:

[0083] (1) The present invention adopts a plurality of hair conditioning agents, namely, a mixture of ginger (Zingiber officinale) root extract, ginseng (Panax ginseng) root extract, kelp (Laminaria japonica) extract, grapefruit (CITRUS GRANDIS) seed extract, Centella asiatica (CENTELLA asiatica) extract, and rice (ORYZA SATIVA) bran extract as hair conditioning agent 1, Thuja orientalis (THUJA orientalis) leaf extract as hair conditioning agent 2, and pyridoxine hydrochloride as hair conditioning agent 3, and by controlling the mass ratio of the three to be 3-5:0.2-0.6:0.1-0.5, the hair loss prevention and hair strengthening effect of the composition is significantly improved; wherein:

[0084] Ginger (Zingiber Officinale) Root Extract: This ingredient can inhibit the activity of Malassezia fungus, reduce scalp inflammation, and regulate sebaceous gland secretion, thereby improving the scalp environment. It has strong antioxidant properties, helping to protect hair from free radical damage, enhancing hair luster and improving its resistance to breakage. Its active ingredients can stimulate the scalp, promote blood circulation, and help improve the nutrient supply to hair follicles.

[0085] Ginseng (PANAX GINSENG) root extract: can increase the expansion of capillaries in the head, thereby providing more nutrition to the hair and enhancing its toughness; helps improve blood circulation in the scalp, promotes the health of hair follicles, and thus reduces hair loss and breakage; by promoting the health of hair follicles, ginseng root extract can enhance the health of hair roots and reduce hair problems caused by malnutrition or poor blood circulation.

[0086] Kelp (LAMINARIA JAPONICA) extract: has antioxidant and anti-aging effects; can inhibit the generation of oxygen free radicals, thereby slowing down the aging process of skin and hair, maintaining the health of scalp and hair, delaying scalp aging, and reducing fine lines and sagging.

[0087] Citrus Grandis seed extract: Rich in B vitamins, protein and other trace elements, it can provide sufficient nourishment for hair, prevent hair loss and promote healthy hair growth; its nutrients have anti-inflammatory and qi-regulating effects, can stimulate hair follicles and accelerate hair growth; it has a strong effect on eliminating free radicals and has anti-aging and antioxidant effects.

[0088] Centella asiatica extract: rich in antioxidants, can reduce hair damage, increase elasticity and prevent breakage; has a soothing and calming effect, can reduce hair loss, while soothing the scalp and building a strong scalp barrier; can promote cell vitality and help hair growth.

[0089] Rice (Oryza sativa) bran extract: This extract improves skin's softness by promoting the production of cholesterol and ceramides, increasing sebum levels. It also possesses antioxidant properties and can activate glutathione reductase, thus aiding in anti-aging efforts. These properties make rice (Oryza sativa) bran extract a promising addition to shampoo products, improving scalp and hair health and reducing hair loss and thinning.

[0090] Thuja orientalis leaf extract: can inhibit scalp oil secretion and reduce hair loss; has antibacterial, moisturizing and astringent effects, and can help the skin lock in moisture; in anti-hair loss shampoo, Thuja orientalis leaf extract is one of the core ingredients, which can inhibit the production of DHT (dihydrotestosterone), thereby reducing hair loss problems, and is especially suitable for people with androgenic alopecia.

[0091] Pyridoxine hydrochloride: can help control oil secretion and make hair more fluffy; contribute to hair health, reduce hair loss, and strengthen hair roots.

[0092] The present invention uses the above-mentioned hair conditioner as the main ingredient to prepare an anti-hair loss and hair strengthening composition that not only has the effect of promoting the health of scalp hair follicles, but also can inhibit scalp oil secretion, reduce hair loss, and promote scalp health and anti-oxidation effects, thereby achieving the effect of preventing hair loss and strengthening hair.

[0093] (2) The present invention uses a mixture of microcrystalline cellulose and glycerol as a suspending agent. The present invention uses a microcrystalline cellulose suspending agent purchased from Vincbio Biotechnology (Guangzhou) Co., Ltd. with the trade name VINCEBIO HPC851. The active ingredient of the microcrystalline cellulose is bacterial microcrystalline cellulose derived from microbial fermentation, with a diameter of only tens of nanometers. It can interweave with each other in liquids such as aqueous solutions to form a nanoscale three-dimensional network structure that is invisible to the naked eye. It can suspend particles with different densities without settling or floating. Due to the unique and stable three-dimensional network structure of the "nanoscale" microcrystalline cellulose, the fine particles of the active ingredient in the liquid system can be locked in the 3D three-dimensional network. This component has good suspending properties, which can not only increase the stability of the system, but also will not significantly increase the viscosity of the system. DETAILED DESCRIPTION

[0094] The following will be combined with the tables in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0095] Hereinafter, examples of the present invention and comparative examples will be shown to explain the composition of the present invention in more detail, but the present invention is not limited thereto.

[0096] The present invention does not limit the sources of the raw materials used. Unless otherwise specified, the raw materials used in the present invention are common commercial products in the technical field.

[0097] The invention discloses a hair loss prevention and hair solidification composition, which comprises the following components by weight percentage: 3-6% hair conditioner, 0.1-1% moisturizer, 0.5-1.0% preservative, 0.01-0.06% colorant, 0.2-0.8% suspending agent and the balance water.

[0098] Preferably, the anti-hair loss and hair solidifying composition comprises the following components by weight percentage: 4-5% hair conditioner, 0.3-0.8% moisturizer, 0.6-0.8% preservative, 0.03-0.05% colorant, 0.3-0.6% suspending agent and the balance water.

[0099] As some preferred embodiments, the hair conditioner has a weight percentage of 3%, 4%, 5% or 6%.

[0100] As some preferred embodiments, the moisturizing agent is present in an amount of 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.7%, 0.8%, 0.9% or 1.0% by weight.

[0101] As some preferred embodiments, the preservative has a weight percentage of 0.5%, 0.6%, 0.7%, 0.8%, 0.9% or 1.0%.

[0102] As some preferred embodiments, the colorant has a weight percentage of 0.01%, 0.02%, 0.03%, 0.04%, 0.05% or 0.06%.

[0103] As some preferred embodiments, the suspending agent has a weight percentage of 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.7% or 0.8%.

[0104] The hair conditioner includes hair conditioner 1;

[0105] The hair conditioner 1 is one or more of ginger (Zingiber officinale) root extract, ginseng (Panax ginseng) root extract, kelp (Laminaria japonica) extract, grapefruit (CITRUS GRANDIS) seed extract, Centella asiatica (CENTELLA asiatica) extract and rice bran (ORYZASATIVA) extract;

[0106] Preferably, the hair conditioner 1 is a mixture of ginger (Zingiber officinale) root extract, ginseng (Panax ginseng) root extract, kelp (Laminaria japonica) extract, grapefruit (CITRUS GRANDIS) seed extract, Centella asiatica (CENTELLA ASIATICA) extract and rice bran (ORYZA SATIVA) extract.

[0107] More preferably, the mass ratio of the ginger (ZINGIBER OFFICINALE) root extract, ginseng (PANAX GINSENG) root extract, kelp (LAMINARIA JAPONICA) extract, grapefruit (CITRUS GRANDIS) seed extract, Centella asiatica (CENTELLA ASIATICA) extract, and rice (ORYZA SATIVA) bran extract is 4-6:4-6:4-6:3-5:0.1-0.5:0.000001.

[0108] Further preferably, the mass ratio of the ginger (ZINGIBER OFFICINALE) root extract, ginseng (PANAXGINSENG) root extract, kelp (LAMINARIA JAPONICA) extract, pomelo (CITRUS GRANDIS) seed extract, Centella asiatica (CENTELLA ASIATICA) extract, and rice (ORYZA SATIVA) bran extract is 5:5:5:3.8:0.25:0.000001.

[0109] More preferably, the hair conditioner 1 is purchased from Guangzhou Huayue Medical Aesthetics Research Institute with the trade name HYFTGF003.

[0110] The hair conditioner further comprises hair conditioner 2;

[0111] The hair conditioning agent 2 is Thuja orientalis leaf extract.

[0112] Preferably, the hair conditioner 2 is purchased from Guangzhou Jianeng Biotechnology Co., Ltd.

[0113] The hair conditioner further comprises hair conditioner 3;

[0114] The hair conditioning agent 3 is pyridoxine hydrochloride.

[0115] Preferably, the hair conditioner 2 is purchased from DSM Nutritional Products Ltd. under the trade name Pyridoxine Hydrochloride.

[0116] As some preferred embodiments, the mass ratio of the hair conditioner 1, hair conditioner 2 and hair conditioner 3 is 3-5:0.2-0.6:0.1-0.5;

[0117] As some more preferred embodiments, the mass ratio of hair conditioner 1, hair conditioner 2 and hair conditioner 3 is 3-4:0.3-0.4:0.2-0.4;

[0118] As some further preferred embodiments, the mass ratio of hair conditioner 1, hair conditioner 2 and hair conditioner 3 is 3.5:0.4:0.25.

[0119] During the implementation process, by controlling the mass ratio of hair conditioner 1, hair conditioner 2 and hair conditioner 3 to 3-5:0.2-0.6:0.1-0.5, the hair loss prevention and hair strengthening effect of the composition can be significantly improved.

[0120] The moisturizing agent is selected from 1,2-pentanediol.

[0121] Preferably, the moisturizing agent is purchased from Guangdong Dimei Biotechnology Co., Ltd. and its trade name is Antimicro 512.

[0122] The preservative is selected from a mixture of phenoxyethanol and ethylhexylglycerin, with a mass ratio of phenoxyethanol and ethylhexylglycerin being 9:1.

[0123] Preferably, the preservative is purchased from Guangdong Dimei New Material Technology Co., Ltd. and its trade name is Antimicro9010.

[0124] The coloring agent is caramel color.

[0125] Preferably, the colorant is purchased from Shandong Aipu Food Industry Co., Ltd. and its trade name is caramel color.

[0126] The above suspending agent is a mixture of microcrystalline cellulose and glycerol.

[0127] Preferably, the suspending agent is a mixture of glycerol and microcrystalline cellulose in a mass ratio of 10:1.

[0128] More preferably, the suspending agent is purchased from Vincen Biotech (Guangzhou) Co., Ltd. with the trade name VINCEBIOHPC851.

[0129] As some preferred embodiments, the anti-hair loss and hair solidifying composition comprises the following components by weight percentage:

[0130]

[0131]

[0132] As a further preferred embodiment, the anti-hair loss and hair solidifying composition comprises the following components by weight percentage:

[0133]

[0134] As a further preferred embodiment, the anti-hair loss and hair solidifying composition comprises the following components by weight percentage:

[0135]

[0136]

[0137] As a preferred embodiment, the anti-hair loss and hair solidifying composition comprises the following components by weight percentage:

[0138]

[0139] As some preferred embodiments, the anti-hair loss and hair solidifying composition comprises the following components by weight percentage:

[0140]

[0141]

[0142] As a preferred embodiment, the anti-hair loss and hair solidifying composition comprises the following components by weight percentage:

[0143]

[0144] The present invention also provides a method for preparing the above-mentioned anti-hair loss and hair solidifying composition, comprising the following steps:

[0145] Heat water to 60-70°C, then add hair conditioner 1 and hair conditioner 2, and homogenize for 1-5 minutes; cool to below 45°C, add the remaining raw materials, continue stirring for 20-40 minutes, stir evenly until no solid particles are left, and cool to obtain the composition.

[0146] The present invention also provides a method for preparing the above-mentioned anti-hair loss and hair solidifying composition, comprising the following steps:

[0147] Heat water to 60-70°C, then add ginger root extract, ginseng root extract, kelp extract, grapefruit seed extract, Centella asiatica extract, rice bran extract and Platycladus orientalis leaf extract, and homogenize for 1-5 minutes; cool to below 45°C, add the remaining raw materials, continue stirring for 20-40 minutes, stir evenly until no solid particles are left, and cool to obtain the composition.

[0148] The present application also provides the use of the above-mentioned anti-hair loss and hair solidification composition in the preparation of an anti-hair loss and hair solidification product;

[0149] The anti-hair loss and hair strengthening product is an anti-hair loss and hair strengthening shampoo product or hair care product, and the shampoo product or hair care product includes but is not limited to shampoo, shampoo, conditioner, and hair care essence.

[0150] A hair loss prevention and hair strengthening shampoo product comprises the above-mentioned hair loss prevention and hair strengthening composition and auxiliary materials.

[0151] Furthermore, an anti-hair loss and hair strengthening shampoo product comprises the above-mentioned anti-hair loss and hair strengthening composition and auxiliary materials used for preparing shampoo.

[0152] Furthermore, an anti-hair loss and hair strengthening shampoo product comprises the above-mentioned anti-hair loss and hair strengthening composition and auxiliary materials used for preparing shampoo.

[0153] A hair loss prevention and hair strengthening product comprises the above-mentioned hair loss prevention and hair strengthening composition and auxiliary materials.

[0154] Furthermore, an anti-hair loss and hair strengthening product comprises the above-mentioned anti-hair loss and hair strengthening composition and auxiliary materials used for preparing hair conditioner.

[0155] Furthermore, an anti-hair loss and hair strengthening product comprises the above-mentioned anti-hair loss and hair strengthening composition and auxiliary materials used for preparing hair care essence.

[0156] The auxiliary materials are selected from one or more of hair conditioners, thickeners, emulsifiers, moisturizers and emollients.

[0157] Example 1: A hair loss prevention and solidification composition and its preparation method

[0158] The formula is shown in Table 1:

[0159] Table 1

[0160] Components content% Ginger root extract 0.15 Ginseng (root extract 0.15 Kelp Extract 0.15 grapefruit seed extract 0.105 Centella asiatica extract 0.0035 Rice bran extract 0.000000035 Platycladus orientalis leaf extract 0.2 Pyridoxine hydrochloride 0.1 1,2-Pentanediol 0.1 Phenoxyethanol 0.45 Ethylhexylglycerin 0.05 caramel color 0.01 VINCEBIO HPC851 0.2 water margin

[0161] Preparation method:

[0162] Heat water to 60-70°C, then add the plant extract at a time and homogenize for 1-5 minutes; cool to below 45°C, add the remaining raw materials, continue stirring for 20-40 minutes, stir evenly until there are no solid particles, and cool to obtain the composition.

[0163] Example 2: An anti-hair loss and hair solidification composition and its preparation method

[0164] The formula is shown in Table 2:

[0165] Table 2

[0166] Components content% Ginger root extract 0.2 Ginseng (root extract 0.2 Kelp Extract 0.2 grapefruit seed extract 0.18 Centella asiatica extract 0.018 Rice bran extract 0.000000035 Platycladus orientalis leaf extract 0.4 Pyridoxine hydrochloride 0.5 1,2-Pentanediol 1 Phenoxyethanol 0.9 Ethylhexylglycerin 0.1 caramel color 0.06 VINCEBIO HPC851 0.8 water margin

[0167] Preparation method:

[0168] Heat water to 60-70°C, then add the plant extract at a time and homogenize for 1-5 minutes; cool to below 45°C, add the remaining raw materials, continue stirring for 20-40 minutes, stir evenly until there are no solid particles, and cool to obtain the composition.

[0169] Example 3: An anti-hair loss and hair solidification composition and its preparation method

[0170] The formula is shown in Table 3:

[0171] Table 3

[0172]

[0173]

[0174] Preparation method:

[0175] Heat water to 60-70°C, then add the plant extract at a time and homogenize for 1-5 minutes; cool to below 45°C, add the remaining raw materials, continue stirring for 20-40 minutes, stir evenly until there are no solid particles, and cool to obtain the composition.

[0176] Example 4: An anti-hair loss and hair solidification composition and its preparation method

[0177] The formula is shown in Table 4:

[0178] Table 4

[0179] Product Name content Hair Conditioner 1 HYFTGF003 3.5％ Hair Conditioner 2 Platycladus orientalis leaf extract 0.4％ Hair Conditioner 3 Pyridoxine hydrochloride 0.25％ moisturizer 1,2-Pentanediol 0.5％ preservative Antimicro 9010 0.6％ caramel color caramel color 0.04％ Suspension Concentrate VINCEBIO HPC851 0.5％ water - margin

[0180] The preparation method is:

[0181] Heat water to 60-70°C, then add hair conditioner 1 and hair conditioner 2, and homogenize for 1-5 minutes; cool to below 45°C, add the remaining raw materials, continue stirring for 20-40 minutes, stir evenly until no solid particles are left, and cool to obtain the composition.

[0182] Example 5: An anti-hair loss and hair solidifying composition and its preparation method

[0183] The formula is shown in Table 5:

[0184] Table 5

[0185] Product Name content Hair Conditioner 1 HYFTGF003 3％ Hair Conditioner 2 Platycladus orientalis leaf extract 0.2％ Hair Conditioner 3 Pyridoxine hydrochloride 0.1％ moisturizer 1,2-Pentanediol 0.1％ preservative Antimicro 9010 0.555％ caramel color caramel color 0.01％ Suspension Concentrate VINCEBIO HPC851 1％ water - margin

[0186] The preparation method is:

[0187] Heat water to 60-70°C, then add hair conditioner 1 and hair conditioner 2, and homogenize for 1-5 minutes; cool to below 45°C, add the remaining raw materials, continue stirring for 20-40 minutes, stir evenly until no solid particles are left, and cool to obtain the composition.

[0188] Example 6: An anti-hair loss and hair solidification composition and its preparation method

[0189] The formula is shown in Table 6:

[0190] Table 6

[0191] Product Name content Hair Conditioner 1 HYFTGF003 4％ Hair Conditioner 2 Platycladus orientalis leaf extract 0.6％ Hair Conditioner 3 Pyridoxine hydrochloride 0.3％ moisturizer 1,2-Pentanediol 1％ preservative Antimicro 9010 0.88％ caramel color caramel color 0.06％ Suspension Concentrate VINCEBIO HPC851 2.0％ water - margin

[0192] The preparation method is:

[0193] Heat water to 60-70°C, then add hair conditioner 1 and hair conditioner 2, and homogenize for 1-5 minutes; cool to below 45°C, add the remaining raw materials, continue stirring for 20-40 minutes, stir evenly until no solid particles are left, and cool to obtain the composition.

[0194] Example 7: An anti-hair loss and hair solidifying composition and its preparation method

[0195] The formula is shown in Table 7:

[0196] Table 7

[0197] Product Name content Hair Conditioner 1 HYFTGF003 3.2％ Hair Conditioner 2 Platycladus orientalis leaf extract 0.3％ Hair Conditioner 3 Pyridoxine hydrochloride 0.2％ moisturizer 1,2-Pentanediol 0.4％ preservative Antimicro 9010 0.62％ caramel color caramel color 0.04％ Suspension Concentrate VINCEBIO HPC851 1.8％ water - margin

[0198] The preparation method is:

[0199] Heat water to 60-70°C, then add hair conditioner 1 and hair conditioner 2, and homogenize for 1-5 minutes; cool to below 45°C, add the remaining raw materials, continue stirring for 20-40 minutes, stir evenly until no solid particles are left, and cool to obtain the composition.

[0200] Example 8: An anti-hair loss and hair solidification composition and its preparation method

[0201] The formula is shown in Table 8:

[0202] Table 8

[0203] Product Name content Hair Conditioner 1 HYFTGF003 0.38％ Hair Conditioner 2 Platycladus orientalis leaf extract 0.42％ Hair Conditioner 3 Pyridoxine hydrochloride 0.56％ moisturizer 1,2-Pentanediol 0.85％ preservative Antimicro 9010 0.75％ caramel color caramel color 0.06％ Suspension Concentrate VINCEBIO HPC851 1.65％ water - margin

[0204] The preparation method is:

[0205] Heat water to 60-70°C, then add hair conditioner 1 and hair conditioner 2, and homogenize for 1-5 minutes; cool to below 45°C, add the remaining raw materials, continue stirring for 20-40 minutes, stir evenly until no solid particles are left, and cool to obtain the composition.

[0206] Comparative Example 1:

[0207] The difference from Example 4 is that no suspending agent is added, and the other aspects are the same as Example 5.

[0208] Comparative Example 2:

[0209] The difference from Example 4 is that the suspending agent is replaced by the traditional suspending agent SF-1, and the rest is the same as Example 5.

[0210] Effect experiment:

[0211] 1. Irritation test - acute eye irritation test

[0212] 1. Materials and methods

[0213] 1.1 Test substances: Experimental group: The samples prepared in Examples 1 to 3 were directly used as test substances.

[0214] 1.2 Experimental animals and breeding environment:

[0215] Experimental animals: 6 New Zealand rabbits (ordinary grade, female, body weight 2.0 - 2.5 kg), animal certificate number: NO.430730241100250913.

[0216] Animal source: Purchased from Hunan Taiping Biotechnology Co., Ltd., experimental animal production license number: SCXK(Xiang)2023 - 0011.

[0217] Feeding environment: Ordinary environment, temperature 21.9 - 23.6 °C, relative humidity 63.7 - 65.8 RH%, experimental animal environment use license number: SYXK(Yue)2023 - 0216.

[0218] Feed: Provided by Jiangquan Feed Factory of Linyi Shengquan Oil and Chemical Industry Co., Ltd., license number: Lu Sizheng(2024)16062, quality certificate number: NO.2024071600177.

[0219] Within 24 h before the test, the two eyes of the above animals were examined (including using sodium fluorescein), and no eye irritation symptoms were found.

[0220] 1.3 Test method

[0221] The above animals were divided into 3 groups, with 2 animals in each group. Gently pull down the lower eyelid of the right eye of the rabbit, and drop 0.1 mL of the test substance into the conjunctival sac, making the upper and lower eyelids close passively for 1 s. Do not rinse the eyes within 24 h after dropping the test substance, and the left eye is not treated as a self - control.

[0222] At 1 h, 24 h, 48 h, 72 h, 4 d, and 7 d after dropping the test substance again, use a magnifying glass and a pupil penlight to examine the eyes of the animals. At the same time, after the 24 - h observation and recording are completed, use sodium fluorescein to examine the eyes of all animals in each examination. Record the eye irritation reaction and calculate the integral according to Table 1 in Part 5 of Chapter 6 of the "Technical Specifications for Cosmetics Safety" (2015 Edition). If no irritation reaction is found within 72 h, the test can be terminated; if corneal involvement or other eye irritation effects are found and do not recover within 7 d, the observation time needs to be extended to 21 d, and observation reports for 7 d, 14 d, and 21 d are required.

[0223] 2. Test results

[0224] See Table 9 below.

[0225] Table 9 Results of the acute eye irritation test of the test substance on rabbits

[0226]

[0227]

[0228] Note: The mean integral value is rounded to 2 decimal places.

[0229] 3. Test conclusion

[0230] The results of the acute eye irritation test of the test substance on rabbits are: under the condition of no rinsing, the highest mean integral values of the test substance on rabbit eyes are 0.00 for the cornea, 0.00 for the iris, and 0.00 for the conjunctiva, all of which are non-irritating.

[0231] 2. Irritation test - multiple skin irritation tests

[0232] 1. Materials and Methods

[0233] 1.1 Test substances: Experimental group: The samples prepared in Examples 4 to 8 were directly used as test substances.

[0234] 1.2 Experimental animals and breeding environment

[0235] Experimental animals: 10 New Zealand rabbits (common grade, male, weighing 2.0-2.5 kg), animal certificate number: NO.44411600015664.

[0236] Animal source: purchased from Guangdong Medical Experimental Animal Center (Sanshui Base), experimental animal production license number: SCXK (Yue) 2023-0035.

[0237] Rearing environment: Standard environment, temperature 21.7-24.1°C, relative humidity 63.2-67.1% RH. Laboratory Animal Environment Use Permit: SYXK(Guangdong)2023-0216.

[0238] Feed: Provided by Jiangquan Feed Factory of Linyi Shengquan Oil and Fat Chemical Co., Ltd., license number: Lu Feed Certificate (2024) 16062, quality certificate number: NO.2024071600177.

[0239] 1.3 Test methods

[0240] Ten adult, healthy rabbits with intact skin were selected and divided into five groups, with two rabbits in each group. 24 hours before the test, the hair on both sides of the rabbits' spine was trimmed without damaging the epidermis. The hair removal area was 3 cm × 3 cm on the left and right sides, and the application area was 2.5 cm × 2.5 cm.

[0241] Apply 0.5 mL of the test substance to the left side of the skin, and an equal amount of distilled water to the right side as a control. Apply once daily for 14 consecutive days. Starting on the second day, shave the hair before each application and remove any residual test substance with warm water. Observe the skin for irritation one hour later and score according to Table 1, Section 4, Chapter 6 of the "Safety Technical Specifications for Cosmetics" (2015 edition). Determine irritation according to Table 2.

[0242] 2. Test results

[0243] See Table 10 below.

[0244] Table 10 Results of multiple skin irritation tests on rabbits

[0245]

[0246]

[0247]

[0248] Note: The mean integral value is rounded to 2 decimal places.

[0249] 3. Test conclusion

[0250] The multiple skin irritation of the test substance to rabbits was as follows: the average score of skin irritation reaction of each animal per day was 0.00, which was non-irritating.

[0251] 3. Human Trial Test

[0252] 1. Materials and methods

[0253] 1.1 Sample: Essence samples prepared in Examples 1-8

[0254] 1.2 Subjects: A total of 80 people, including 64 males and 16 females, with 10 people in each group (8 males and 2 females), aged 38-55 years old, who met the volunteer inclusion criteria.

[0255] 1.3 Trial Method: Have the subjects use the test product directly according to the usage characteristics and methods indicated on the cosmetic product label. Observe or follow up by phone once a week. The subjects' skin reactions should be recorded according to the grading standards for adverse skin reactions in human trial tests in the "Technical Specifications for Safety of Cosmetics" (2015 edition). The trial period should be no less than 4 weeks.

[0256] 2. The test results are shown in Table 11 below.

[0257] Table 11 Results of human trials of anti-hair loss cosmetics

[0258]

[0259] According to the test results in Table 11 above: 0 out of 80 people had adverse skin reactions.

[0260] 4. Anti-hair loss efficacy test

[0261] 1 Test product: sample prepared in Example 4 (dark brown liquid).

[0262] 2. Control product: A base formula product (brown liquid) of the corresponding test product that does not contain anti-hair loss ingredients, tested in parallel with the test product.

[0263] 3. Washout period product: Same as the control product. Both the experimental group and the control group use the control product during the washout period.

[0264] 4 Subjects: The number of valid subjects who completed the test was 66, the planned number of subjects in the experimental group was 33, the actual number of valid subjects was 33, the planned number of subjects in the control group was 33, the actual number of valid subjects was 33, including 99 males and 7 females, aged 20 to 50 years old, with an average age of 34.6+9.8 years old, which met the voluntary selection criteria for the subjects.

[0265] 5. Test equipment: Professional digital camera; pixel count not less than 15 million; parameters such as aperture, ISO, and focal length should remain consistent throughout the test; Image capture stand: A device capable of fixing the subject's head so that the top of the head and the camera lens remain perpendicular, and used with a professional digital camera to capture a full picture of the hair centered on the top of the head; Dermatoscope: LED light source, wavelength 450-750nm, color temperature 6500-8000k, illumination not less than 540lx, magnification ≥20x, detection diameter not less than 1.0cm or area not less than 0.8cm 2 Comb: The tooth density is moderate (tooth spacing 0.9-1.1mm), the tooth length is 2.0-3.0cm, and the comb length is not less than 10cm (excluding the comb handle). Combs of the same specifications and materials must be used throughout the test. After each use, they must be disinfected in accordance with the relevant requirements of the "Technical Specifications for Disinfection of Medical Institutions" (WS-T367-2012).

[0266] 6 Test method: The test was conducted in accordance with the specific requirements of the "Technical Specifications for Safety of Cosmetics" (2015 edition). Volunteer subjects were recruited according to the inclusion and exclusion criteria, and the 60-combing method was used to count hair loss and record the results. Qualified subjects underwent a 2-week washout period, and after the washout period, the 60-combing method was performed again. Only those with a hair loss count of more than 10 hairs could enter the formal test. The subjects were divided into the test product group and the control product group according to the random grouping method. The subjects' hair baseline values before the test product were evaluated, including hair loss count, hair density assessment, and image capture, and the results were recorded. The product was used continuously for 12 weeks according to the product instructions, and the same evaluation and testing were performed again after 4, 8, and 12 weeks of use.

[0267] 7 Statistical Methods

[0268] Statistical analysis software was used for data analysis. Measurement data were expressed as mean ± standard deviation and tested for normal distribution. Comparisons before and after treatment with the same sample were performed using the paired t-test. Comparisons before and after treatment with graded materials were performed using the two-sample rank sum test. Comparisons between the experimental samples and the control group were performed using the independent sample t-test or rank sum test. All statistical analyses were two-tailed, with a significance level of α = 0.05.

[0269] 8 Data Analysis

[0270] See Tables 12-13 below.

[0271] Table 12 Significance analysis of different time points before and after use of the test product

[0272]

[0273]

[0274] Note: “NS” means P ≥ 0.05, no significant difference; “S” means P < 0.05, there is a significant difference.

[0275] Table 13 Significance analysis of the difference between the test product and the control product before and after use

[0276]

[0277] Note: “NS” means P ≥ 0.05, no significant difference; “S” means P < 0.05, there is a significant difference.

[0278] 9. Test conclusion:

[0279] The results of the anti-hair loss efficacy test showed that after using the test product for 4 weeks, 8 weeks, and 12 weeks, there was no significant increase in the hair loss count compared with week 0; and after using it for 8 weeks and 12 weeks, the hair loss count was significantly reduced (P < 0.05); after using the test product for 4 weeks, 8 weeks, and 12 weeks, there was no significant decrease in the overall hair density compared with week 0; after using the test product for 4 weeks, 8 weeks, and 12 weeks, there was no significant decrease in the local hair density compared with week 0.

[0280] Compared with the control product, the difference in hair loss counts of the test product decreased significantly after 4, 8 and 12 weeks of use (P < 0.05); the difference in overall hair density increased significantly after 8 weeks of use (P < 0.05); the difference in local hair density increased significantly after 8 and 12 weeks of use (P < 0.05), indicating that the test sample has anti-hair loss effect.

[0281] 5. Stability test

[0282] In order to ensure the performance of the product during storage and use, and to prevent physical and chemical changes, and to avoid phenomena such as oil floating and stratification that may affect consumer trials, high-temperature and low-temperature storage stability tests will be conducted on the product.

[0283] Laboratory storage stability tests at high and low temperatures were conducted using the following method: Over a 48-hour period, the temperature was raised and lowered between -20°C and 40°C at a rate of 2°C per hour, repeated repeatedly, and then observed after returning to room temperature. The results are shown in Table 14.

[0284] Table 14

[0285] stability Example 1 No oil floating or stratification Example 2 No oil floating or stratification Example 3 No oil floating or stratification Example 4 No oil floating or stratification Example 5 No oil floating or stratification Example 6 No oil floating or stratification Example 7 No oil floating or stratification Example 8 No oil floating or stratification Comparative Example 1 Obvious oil slick and stratification phenomena Comparative Example 2 Slight oil slick and stratification

[0286] According to the test results in Table 14 above, it can be seen that the anti-hair loss and hair solidifying compositions prepared in Examples 1-8 of the present invention have good high and low temperature stability. No oil floating or stratification occurs after circulating between -20°C and 40°C for 48 hours.

[0287] In Comparative Example 1, no suspending agent was added, and the stability of the obtained anti-hair loss and hair solidifying composition was significantly reduced. After 30 hours of high and low temperature cycles, stratification occurred, affecting user satisfaction.

[0288] The conventional suspending agent used in Comparative Example 2 also reduces the stability of the obtained anti-hair loss and hair solidifying composition. After 48 hours of high and low temperature cycling, slight stratification will occur, but it does not affect the use.

[0289] The reason is that the active ingredient of the microcrystalline cellulose suspension used in the present invention is bacterial microcrystalline cellulose derived from microbial fermentation, which has a diameter of only tens of nanometers. It can interweave with each other in liquids such as aqueous solutions to form a nanoscale three-dimensional network structure that is invisible to the naked eye. It can suspend particles with different densities without settling or floating. Due to the unique and stable "nanoscale" three-dimensional network structure of microcrystalline cellulose, the fine particles of active ingredients in the liquid system can be locked in the 3D three-dimensional network. This component has excellent suspension properties, which not only increases the stability of the system, but also does not significantly increase the viscosity of the system.

[0290] 6. Moisturizing effect test

[0291] Skin moisture content (Mean moisture value, MMV) test

[0292] Test principle: This method uses the capacitance method to measure the moisture content of the stratum corneum of human skin. The principle is based on the significant difference in the dielectric constants of water and other substances. According to the different water content of the skin, the measured capacitance value of the skin is different. The observed parameters can represent the scalp moisture value.

[0293] Testing equipment: Skin moisture content tester

[0294] Test environment: 22℃±1℃, humidity 50%±5%.

[0295] Subjects: 100 subjects aged 20-50 were selected and divided into 10 groups, with 10 subjects in each group. The age distribution of subjects in each group was consistent, the skin condition was basically the same, and no hormone drugs or immunosuppressants had been used in the past month.

[0296] Testing Method: Subjects applied the anti-hair loss and hair strengthening compositions provided in Examples 1-8 and Comparative Examples 1-2 to their scalps twice daily for four consecutive weeks. A skin moisture content meter was used to measure scalp moisture content before application, one week, two weeks, and four weeks after application. Testing was also performed two hours after each application of the anti-hair loss and hair strengthening compositions, and the average scalp moisture content was calculated. The measurement results are shown in Tables 15 and 16.

[0297] Water content change rate = (scalp water content after n weeks of use - scalp water content before use) / scalp water content before use * 100%.

[0298] Table 15 Scalp moisture content at different times

[0299]

[0300]

[0301] Table 16 Change rate of scalp moisture content at different times

[0302]

[0303] The test results in Tables 15 and 16 indicate that the anti-hair loss and hair-fixing compositions prepared in Examples 1-8 of the present invention exhibit excellent hydration and moisture retention. For example, the anti-hair loss and hair-fixing compositions prepared in Examples 1-3 all increased scalp moisture content by more than 1.5% after one week of use, more than 2.5% after two weeks of use, and more than 3% after four weeks of use.

[0304] The anti-hair loss and hair solidifying compositions prepared in Examples 4-8 have better moisturizing and water retention effects. After one week of use, the water content of the scalp increases by more than 1.5%, and the maximum increase can reach 1.66%. After two weeks of use, the water content of the scalp increases by more than 2.8%, and the maximum can reach 3.05%. After four weeks of use, the water content of the scalp can increase by more than 3.5%, and the maximum can reach 3.81%.

[0305] In Comparative Example 1, no suspending agent is added, and the moisturizing and water-retaining effects of the obtained anti-hair loss and hair-fixing composition are significantly reduced. Since the present invention adds a microcrystalline cellulose suspending agent, the active ingredient of the microcrystalline cellulose is bacterial microcrystalline cellulose derived from microbial fermentation, with a diameter of only tens of nanometers. It can be interwoven with each other in liquids such as aqueous solutions to form a nanoscale three-dimensional network structure that is invisible to the naked eye. Due to the unique and stable three-dimensional network structure of microcrystalline cellulose at the "nanoscale", the fine particles of active ingredients in the liquid system can be locked in the 3D network, thereby achieving a better water-retaining effect. In addition, the microcrystalline cellulose suspending agent has relatively high biocompatibility and is hydrophilic, which can better transport the effective ingredients such as plant extracts in the anti-hair loss and hair-fixing composition into the skin, thereby increasing the skin's water content and achieving a moisturizing effect.

[0306] In Comparative Example 2, a conventional suspending agent is used, and the moisturizing and water-retaining effects of the obtained anti-hair loss and hair-fixing composition are also lower than those of the embodiment, because the component can only play a suspending effect and cannot achieve the moisturizing and water-retaining effects.

[0307] The above embodiments are preferred implementation modes of the present invention, but the implementation modes of the present invention are not limited to the above embodiments. Any other changes, modifications, substitutions, combinations, and simplifications that do not deviate from the spirit and principles of the present invention should be considered as equivalent replacement methods and are included in the scope of protection of the present invention.

Claims

1. An anti-hair loss and hair strengthening composition, characterized in that: The composition comprises the following components by weight percentage: 3-6% hair conditioner, 0.1-1% moisturizer, 0.5-1.0% preservative, 0.01-0.06% colorant, 0.2-0.8% suspending agent and the balance water; Wherein, the hair conditioner is a mixture of hair conditioner 1, hair conditioner 2 and hair conditioner 3, with a mass ratio of 3-5:0.2-0.6:0.1-0.5; The hair conditioner 1 comprises one or more of ginger root extract, ginseng root extract, kelp extract, grapefruit seed extract, centella asiatica extract, and rice bran extract; The hair conditioner 2 is Platycladus orientalis leaf extract; The hair conditioning agent 3 is pyridoxine hydrochloride.

2. The anti-hair loss and solidifying composition according to claim 1, wherein: The hair conditioner 1 is a mixture of ginger root extract, ginseng root extract, kelp extract, grapefruit seed extract, Centella asiatica extract and rice bran extract.

3. The anti-hair loss and solidifying composition according to claim 2, wherein: The mass ratio of the ginger root extract, ginseng root extract, kelp extract, grapefruit seed extract, Centella asiatica extract and rice bran extract is 4-6:4-6:4-6:3-5:0.1-0.5:0.000001.

4. The anti-hair loss and solidifying composition according to claim 3, wherein: The mass ratio of the ginger root extract, ginseng root extract, kelp extract, grapefruit seed extract, Centella asiatica extract and rice bran extract is 5:5:5:3.8:0.25:0.000001.

5. The anti-hair loss and solidifying composition according to claim 1, wherein: The hair conditioner 1 was purchased from Guangzhou Huayue Medical Aesthetics Research Institute with the trade name HYFTGF003.

6. The anti-hair loss and solidifying composition according to claim 1, wherein: The hair conditioner 2 is purchased from DSM Nutritional Products Ltd. under the trade name Pyridoxine Hydrochloride.

7. The anti-hair loss and solidifying composition according to claim 1, wherein: The mass ratio of the hair conditioner 1, the hair conditioner 2 and the hair conditioner 3 is 3-4:0.3-0.4:0.2-0.

4.

8. The anti-hair loss and solidifying composition according to claim 7, characterized in that: The mass ratio of the hair conditioner 1, hair conditioner 2 and hair conditioner 3 is 3.5:0.4:0.

25.

9. The anti-hair loss and solidifying composition according to claim 1, wherein: The moisturizing agent is 1,2-pentanediol.

10. The anti-hair loss and solidifying composition according to claim 1, characterized in that: The suspending agent is a mixture of microcrystalline cellulose and glycerol.

11. The anti-hair loss and solidifying composition according to claim 10, characterized in that: The suspending agent is a mixture of glycerol and microcrystalline cellulose in a ratio of 10:

1.

12. The anti-hair loss and solidifying composition according to any one of claims 1 to 11, characterized in that: The anti-hair loss and hair solidifying composition comprises the following components by weight percentage:

13. The anti-hair loss and solidifying composition according to claim 12, characterized in that: The anti-hair loss and hair solidifying composition comprises the following components by weight percentage:

14. The anti-hair loss and solidifying composition according to claim 13, characterized in that: The anti-hair loss and hair solidifying composition comprises the following components by weight percentage:

15. The anti-hair loss and solidifying composition according to any one of claims 1 to 11, characterized in that: The anti-hair loss and hair solidifying composition comprises the following components by weight percentage:

16. The anti-hair loss and solidifying composition according to claim 15, characterized in that: The anti-hair loss and hair solidifying composition comprises the following components by weight percentage:

17. The method for preparing the anti-hair loss and hair solidifying composition according to any one of claims 15 to 16, characterized in that: The steps include: Heat water to 60-70°C, then add hair conditioner 1 and hair conditioner 2, and homogenize for 1-5 minutes; cool to below 45°C, add the remaining raw materials, continue stirring for 20-40 minutes, stir evenly until no solid particles are left, and cool to obtain the composition.

18. The method for preparing the anti-hair loss and solidifying composition according to any one of claims 12 to 14, characterized in that: The steps include: Heat water to 60-70°C, then add ginger root extract, ginseng root extract, kelp extract, grapefruit seed extract, Centella asiatica extract, rice bran extract and Platycladus orientalis leaf extract, and homogenize for 1-5 minutes; cool to below 45°C, add the remaining raw materials, continue stirring for 20-40 minutes, stir evenly until no solid particles are left, and cool to obtain the composition.

19. Use of the anti-hair loss and hair solidifying composition according to any one of claims 1 to 16 in the preparation of an anti-hair loss and hair solidifying product.

20. The use according to claim 16, characterized in that: The anti-hair loss and hair strengthening product is an anti-hair loss and hair strengthening shampoo product or a hair care product, and the shampoo product or hair care product is shampoo, shampoo, conditioner, or hair care essence.

21. An anti-hair loss and hair strengthening shampoo product, characterized by: The invention comprises the anti-hair loss and solidifying composition according to any one of claims 1 to 16 and auxiliary materials.

22. A hair loss prevention and strengthening hair care product, characterized by: The invention comprises the anti-hair loss and solidifying composition according to any one of claims 1 to 16 and auxiliary materials.

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