Eco-friendly formulations for inducing plant system resistance to control pepper anthracnose

Abstract

The invention discloses an eco-friendly preparation for controlling pepper anthracnose by inducing plant system resistance, and belongs to the technical field of plant disease prevention and control, in the eco-friendly preparation, S-allyl-L-cysteine is a sulfur-containing amino acid derivative and has the effect of inducing plants to generate disease-resistant substances such as disease course-related proteins, and S-allyl-L-cysteine is a sulfur-containing amino acid derivative. Therefore, the systematic resistance of the plant is improved. 2-piperidinecarboxylic acid inhibits the growth and reproduction of pathogenic bacteria by influencing the metabolic pathway of the pathogenic bacteria. The sodium dodecyl sulfate is beneficial to uniform distribution and absorption of the preparation, and the utilization efficiency of the effective components is improved. The combination of potassium glutamate and S-allyl-L-cysteine can enhance the direct defense reaction and systematic acquired resistance of plants at the same time, and a multi-layer protective barrier is formed. The 1, 2-piperidinecarboxylic acid enables plants to maintain a healthy state even when the plants are attacked by pathogenic bacteria. Due to the addition of sodium dodecyl sulfate, the effective components can be quickly and uniformly absorbed by plants, and the disease-resistant effect of the effective components can be exerted to the greatest extent.

Description

Technical Field

[0001] The present invention belongs to the technical field of plant disease prevention and control, and specifically relates to an eco-friendly preparation for inducing plant systemic resistance to control pepper anthracnose. Background Art

[0002] With the rapid development of modern agriculture, crop diseases are becoming increasingly prominent, particularly fungal diseases such as pepper anthracnose, which have caused significant losses to agricultural production. Traditionally, chemical pesticides have been widely used to combat these diseases. However, the long-term and extensive use of chemical pesticides not only leads to the development of resistance in pathogens but also seriously pollutes soil and water sources, disrupting ecological balance and posing a threat to human health and environmental protection. In recent years, with increasing attention paid to ecological and food safety, the development of environmentally friendly and highly effective biopesticides has become a hot topic in agricultural research. Plant-induced resistance, a method that harnesses a plant's own defense mechanisms to resist pathogens, offers advantages such as environmental friendliness, safety, and longevity.

[0003] However, the existing induced resistance preparations on the market often have single ingredients, limited effects, and lack the effect of improving the soil environment, and cannot meet the comprehensive needs of modern agricultural production. Summary of the Invention

[0004] The object of the present invention is to provide an eco-friendly formulation for inducing plant systemic resistance to control pepper anthracnose in order to solve the above-mentioned problems.

[0005] The technical solution adopted by the present invention is as follows: an eco-friendly preparation for inducing plant systemic resistance to control pepper anthracnose, characterized in that the eco-friendly preparation comprises: potassium glutamate: 10-20 parts by weight; S-allyl-L-cysteine: 8-15 parts by weight; 2-piperidinic acid: 5-10 parts by weight; sodium lauryl sulfonate: 3-6 parts by weight; citric acid: 1-3 parts by weight; polyethylene glycol: 3-6 parts by weight; copper sulfate: 0.3-0.8 parts by weight; diatomaceous earth: 6-10 parts by weight; seaweed extract: 8-12 parts by weight; chitosan: 4-7 parts by weight; sodium iron EDTA: 0.5-1.5 parts by weight; vitamin E: 0.5-1.5 parts by weight; mannitol: 2-5 parts by weight; gingerol extract: 3-6 parts by weight; oregano essential oil: 1-3 parts by weight; and cinnamon essential oil: 1-3 parts by weight.

[0006] In a preferred embodiment, the preparation method of the preparation comprises the following steps:

[0007] S1: Weigh potassium glutamate, S-allyl-L-cysteine, 2-piperidinic acid, sodium lauryl sulfate, citric acid, polyethylene glycol, copper sulfate, diatomaceous earth, seaweed extract, chitosan, sodium iron EDTA, vitamin E, mannitol, gingerol extract, oregano essential oil and cinnamon essential oil according to the weight parts range in the above formula for later use;

[0008] S2: Weighed solid ingredients: potassium glutamate, S-allyl-L-cysteine, 2-piperidinic acid, sodium lauryl sulfate, citric acid, polyethylene glycol, copper sulfate, diatomaceous earth, seaweed extract, chitosan, sodium iron EDTA, vitamin E, mannitol, and gingerol extract are placed in a mixer and mixed evenly;

[0009] S3: Slowly add the liquid ingredients: oregano essential oil and cinnamon essential oil to the solid ingredients in the blender while continuously stirring to ensure uniform mixing;

[0010] S4: Continue stirring the mixed solid and liquid components until a uniform suspension or solution is formed;

[0011] S5: After the mixture is cooled to room temperature, gently stir;

[0012] S6: diluting the mixed solution to the desired concentration according to actual use requirements;

[0013] S7: Perform quality checks on the diluted preparation, including pH value, viscosity, and suspension rate, to ensure that the preparation meets the requirements;

[0014] S8: Place qualified products into appropriate containers and seal them for storage;

[0015] S9: Mark the product name, ingredients, instructions for use, storage conditions, production date and expiration date on the product packaging.

[0016] In a preferred embodiment, in step S1, an electronic balance with an accuracy of 0.01 g is used to accurately weigh the ingredients strictly in accordance with the weight range specified in the recipe; after each ingredient is weighed, it is immediately transferred to a clean, dry container to avoid moisture absorption or cross contamination; all weighing operations are performed in an environment with a room temperature of 20-25°C and a relative humidity of less than 50%.

[0017] In a preferred embodiment, in step S2, the speed of the high-speed stirrer is set to 250 rpm, and the mixing process lasts for 15 minutes to ensure that all solid components are completely dispersed and evenly mixed; after the mixing is completed, the wall of the mixing container is thoroughly cleaned with a scraper to ensure that there is no residue.

[0018] In a preferred embodiment, in step S3, during the injection process, the stirring speed is reduced to 150 rpm to ensure that the liquid component and the solid component are fully integrated and avoid local aggregation; after the injection is completed, stirring is continued for 10 minutes to ensure that the mixture is uniform.

[0019] In a preferred embodiment, in step S4, homogenization is performed at a pressure of 50-60 bar for 5 minutes; after the homogenization is completed, the uniformity and stability of the mixture are checked to ensure that a stable suspension or solution is formed; if stratification or precipitation is found, the mixture needs to be homogenized again until it is qualified.

[0020] In a preferred embodiment, in step S5, the mixture is naturally cooled at room temperature of 20-25°C for 30 minutes until the temperature of the mixture drops below 25°C; after cooling, a low-speed stirrer is used at a speed not exceeding 50 rpm for stirring for 5 minutes.

[0021] In a preferred embodiment, in step S6, deionized water is used for dilution, and water is slowly added to the mixture at a rate of 100 ml per minute using a peristaltic pump, while continuous stirring is performed using a low-speed stirrer at a speed of 100 rpm; the dilution multiple is strictly carried out in accordance with the instructions to ensure that the concentration of the diluted preparation is accurate;

[0022] In step S7, a pH meter is used to measure the pH value to ensure that it is within the range of 5.0-7.0; a viscometer is used to measure the viscosity to ensure that it meets the design requirements; a suspension rate meter is used to measure the suspension rate to ensure that it reaches more than 95%; all test items must meet the preset standards, and unqualified products will be discarded.

[0023] In a preferred embodiment, in step S8, after filling is completed, the container is immediately sealed using an aluminum foil sealing machine to ensure that the container is well sealed and leak-free; the sealed container is visually inspected to ensure that it is free of damage and contamination.

[0024] In a preferred embodiment, in step S9, the packaged product is subjected to a final quality inspection, including appearance inspection, label inspection, and sealing inspection; a magnifying glass with a magnification of 10 times is used to check whether there are stains or damage on the bottle body and label; a sealing tester is used to check the sealing of the bottle cap to ensure that there is no leakage; all inspection items must meet the quality standards, and unqualified products will be rejected; quality inspection is carried out in a dedicated quality inspection area, and the ambient temperature is controlled at 23±2°C.

[0025] In summary, due to the adoption of the above technical solution, the beneficial effects of the present invention are:

[0026] 1. In the present invention, potassium glutamate can provide the nitrogen source required for plant growth while stimulating the activity of defense enzymes in the plant body, thereby enhancing the plant's resistance to pathogens. S-allyl-L-cysteine is a sulfur-containing amino acid derivative that can induce plants to produce disease-resistant substances, such as pathogenesis-related proteins, thereby improving the plant's systemic resistance. 2-Nipecotic acid inhibits the growth and reproduction of pathogens by affecting their metabolic pathways. Sodium lauryl sulfate, as a surfactant, facilitates the uniform distribution and absorption of the formulation, improving the utilization efficiency of the active ingredients. The combination of potassium glutamate and S-allyl-L-cysteine can simultaneously enhance the plant's direct defense response and systemic acquired resistance, forming a multi-layered protective barrier. The addition of 2-Nipecotic acid further weakens the pathogen's ability to infect, allowing the plant to remain healthy even when attacked by pathogens. The addition of sodium lauryl sulfate ensures that these active ingredients are quickly and evenly absorbed by the plant, maximizing their disease-fighting effects. This synergistic effect makes the formulation perform well in controlling pepper anthracnose, effectively reducing the occurrence and spread of the disease.

[0027] 2. In the present invention, the diatomaceous earth, seaweed extract, and chitosan components of the formulation not only have disease-fighting effects but also improve the soil environment. Diatomaceous earth increases soil air permeability and water retention, promoting root development. Seaweed extract and chitosan, rich in organic matter and trace elements, can improve soil fertility and promote crop growth. The combined effect of these components not only improves the soil environment but also increases crop yield and quality, achieving sustainable agricultural development.

[0028] 3. In the present invention, the design of the preparation fully considers eco-friendliness. All ingredients are environmentally friendly and will not pollute the soil and water sources. By inducing plant resistance and improving the soil environment to control diseases, dependence on chemical pesticides is reduced, which is conducive to protecting the ecological environment. The addition of ingredients such as polyethylene glycol and mannitol improves the stability and effectiveness of the preparation, reduces the amount used, and further reduces the environmental burden. Natural ingredients such as vitamin E, gingerol extract, oregano essential oil and cinnamon essential oil not only have disease resistance, but also have certain insect repellent and antibacterial effects, reducing the use of pesticides. This eco-friendly design concept not only meets the needs of modern agricultural production, but also provides strong support for the sustainable development of agriculture, achieving a win-win situation for disease control and environmental protection. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1 It is a schematic diagram of the process principle of the present invention. DETAILED DESCRIPTION

[0030] In order to make the purpose, technical solutions and advantages of the present invention more clearly understood, the present invention will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention.

[0031] Reference Figure 1 ,

[0032] Example:

[0033] An eco-friendly formulation for inducing plant systemic resistance to control pepper anthracnose, the eco-friendly formulation comprising: potassium glutamate: 10 parts by weight; S-allyl-L-cysteine: 8 parts by weight; 2-piperidinic acid: 5 parts by weight; sodium lauryl sulfonate: 3 parts by weight; citric acid: 13 parts by weight; polyethylene glycol: 3 parts by weight; copper sulfate: 0.3 parts by weight; diatomaceous earth: 6 parts by weight; seaweed extract: 8 parts by weight; chitosan: 4 parts by weight; sodium iron EDTA: 0.5 parts by weight; vitamin E: 0.5 parts by weight; mannitol: 2 parts by weight; gingerol extract: 3 parts by weight; oregano essential oil: 1 part by weight; and cinnamon essential oil: 1 part by weight.

[0034] The preparation method of the preparation comprises the following steps:

[0035] S1: Weigh potassium glutamate, S-allyl-L-cysteine, 2-piperidinic acid, sodium lauryl sulfate, citric acid, polyethylene glycol, copper sulfate, diatomaceous earth, seaweed extract, chitosan, sodium iron EDTA, vitamin E, mannitol, gingerol extract, oregano essential oil and cinnamon essential oil according to the weight parts range in the above formula for later use;

[0036] S2: Place the weighed solid ingredients (potassium glutamate, S-allyl-L-cysteine, 2-piperidinic acid, sodium lauryl sulfate, citric acid, polyethylene glycol, copper sulfate, diatomaceous earth, seaweed extract, chitosan, sodium iron EDTA, vitamin E, mannitol, gingerol extract) into a mixer and mix well;

[0037] S3: Slowly add the liquid ingredients (oregano essential oil and cinnamon essential oil) to the solid ingredients in the mixer while continuously stirring to ensure uniform mixing;

[0038] S4: Continue stirring the mixed solid and liquid components until a uniform suspension or solution is formed;

[0039] S5: After the mixture is cooled to room temperature, gently stir;

[0040] S6: diluting the mixed solution to a desired concentration, usually according to actual use requirements;

[0041] S7: Perform quality checks on the diluted preparation, including pH value, viscosity, suspension rate, etc., to ensure that the preparation meets the requirements;

[0042] S8: Place qualified products into appropriate containers and seal them for storage;

[0043] S9: Mark the product name, ingredients, instructions for use, storage conditions, production date and expiration date on the product packaging.

[0044] In step S1, use an electronic balance with an accuracy of 0.01 g to accurately weigh the ingredients within the weight range specified in the recipe. After weighing each ingredient, immediately transfer it to a clean, dry container to avoid moisture absorption and cross-contamination. All weighing operations should be performed at a room temperature of 20-25°C and a relative humidity below 50%.

[0045] In step S2, the speed of the high-speed stirrer is set to 250 rpm, and the mixing process is continued for 15 minutes to ensure that all solid components are completely dispersed and mixed evenly. After the mixing is completed, the walls of the mixing container are thoroughly cleaned with a scraper to ensure that there is no residue.

[0046] In step S3, during the injection process, the stirring speed was reduced to 150 rpm to ensure that the liquid component and the solid component were fully integrated and local aggregation was avoided. After the injection was completed, stirring was continued for 10 minutes to ensure that the mixture was uniform.

[0047] In step S4, homogenization is performed at a pressure of 50-60 bar for 5 minutes. After homogenization, the mixture is checked for uniformity and stability to ensure that a stable suspension or solution has been formed. If stratification or precipitation is observed, the mixture is homogenized again until it passes the homogenization.

[0048] In step S5, the mixture is cooled naturally at room temperature (20-25° C.) for 30 minutes until the temperature of the mixture drops below 25° C. After cooling, the mixture is stirred for 5 minutes using a low-speed stirrer (the speed does not exceed 50 rpm).

[0049] In step S6, deionized water was slowly added to the mixture at a rate of 100 ml per minute using a peristaltic pump while continuously stirring using a low-speed stirrer (100 rpm). The dilution ratio was strictly in accordance with the instructions to ensure the correct concentration of the diluted preparation.

[0050] In step S7, the pH value is measured using a pH meter to ensure it is within the range of 5.0-7.0; the viscosity is measured using a viscometer to ensure it meets design requirements; and the suspension rate is measured using a suspension rate meter to ensure it is above 95%. All test items must meet the preset standards, and unqualified products will be discarded.

[0051] In step S8, after filling is completed, the container is immediately sealed using an aluminum foil sealer to ensure that the container is well sealed and leak-free. The sealed container is visually inspected to ensure that there is no damage or contamination.

[0052] In step S9, the packaged products are subjected to final quality inspection, including appearance inspection, label inspection, and sealing inspection. Use a magnifying glass (magnification: 10x) to check whether the bottle body and label are stained or damaged; use a sealing tester (model: XX-MF20, test pressure set to 20,000-30 kPa) to check the sealing of the bottle cap to ensure there is no leakage. All inspection items must meet quality standards, and unqualified products will be rejected. Quality inspection is carried out in a dedicated quality inspection area, and the ambient temperature is controlled at 23±2°C.

[0053] Test example: Evaluate the efficacy of an eco-friendly formulation against pepper anthracnose and its impact on crop growth. 1. Test objective:

[0054] Verify the beneficial effects of the above-mentioned eco-friendly preparations in preventing and controlling pepper anthracnose, enhancing plant disease resistance, improving soil environment and increasing crop yields.

[0055] 2. Test materials:

[0056] Eco-friendly formulation (prepared according to the above formula)

[0057] Pepper variety: XX variety

[0058] Control group: conventional chemical pesticides

[0059] Experimental field: divided into experimental area and control group, each with an area of 1 mu

[0060] 3. Test methods:

[0061] The same number of pepper plants were planted in the experimental area and the control group.

[0062] The test area was sprayed with eco-friendly formulations according to the recommended dosage for a total of three times with an interval of 10 days.

[0063] The control group used conventional chemical pesticides, sprayed according to the recommended dosage, for a total of 3 times with an interval of 10 days.

[0064] Record the incidence of pepper anthracnose, plant growth and yield.

[0065] 4. Test data recording and analysis:

[0066] The following is an example of a test data table:

[0067] Control effect (%) = [1-(disease index before treatment in control area × disease index after treatment in treatment area) / (disease index after treatment in control area × disease index before treatment in treatment area)] × 100

[0068]

[0069]

[0070] 5. Test conclusion:

[0071] The above test data demonstrates that the eco-friendly formulation is significantly effective in preventing and controlling pepper anthracnose, with a significantly lower incidence rate than the control group. Furthermore, plants in the experimental area experienced more vigorous growth, more leaves, and significantly increased yield. Furthermore, the soil environment in the experimental area improved, with increased organic matter content, enhanced air permeability, and an increase in the number of beneficial microorganisms. In summary, this eco-friendly formulation has the beneficial effects of enhancing plant disease resistance, improving the soil environment, and increasing crop yields, meeting the needs of modern agricultural production and providing strong support for sustainable agricultural development.

[0072] From the above experimental data, we can know that:

[0073] In the present invention, potassium glutamate can provide the nitrogen source required for plant growth while stimulating the activity of the plant's defense enzyme system, thereby enhancing the plant's resistance to pathogens. S-allyl-L-cysteine is a sulfur-containing amino acid derivative that has the effect of inducing plants to produce disease-resistant substances such as pathogenesis-related proteins, thereby improving the plant's systemic resistance. 2-piperidinic acid inhibits the growth and reproduction of pathogens by affecting their metabolic pathways. Sodium lauryl sulfate, as a surfactant, helps to evenly distribute and absorb the formulation, improving the utilization efficiency of the active ingredients. The combination of potassium glutamate and S-allyl-L-cysteine can simultaneously enhance the plant's direct defense response and systemic acquired resistance, forming a multi-layered protective barrier. The addition of 2-piperidinic acid further weakens the pathogen's ability to infect, allowing the plant to remain healthy even when attacked by pathogens. The addition of sodium lauryl sulfate ensures that these active ingredients can be quickly and evenly absorbed by the plant, maximizing their disease-fighting effects. This synergistic effect makes the formulation perform well in controlling pepper anthracnose, effectively reducing the occurrence and spread of the disease.

[0074] In the present invention, the ingredients in the formulation, such as diatomaceous earth, seaweed extract, and chitosan, not only have disease-fighting effects but also improve the soil environment. Diatomaceous earth increases soil permeability and water retention, promoting root development. Seaweed extract and chitosan, rich in organic matter and trace elements, can improve soil fertility and promote crop growth. The combined effect of these ingredients not only improves the soil environment but also increases crop yield and quality, achieving sustainable agricultural development.

[0075] In the present invention, the design of the preparation fully considers eco-friendliness, and all ingredients are environmentally friendly and will not pollute soil and water sources. Diseases are controlled by inducing plant resistance and improving the soil environment, reducing dependence on chemical pesticides and helping to protect the ecological environment. The addition of ingredients such as polyethylene glycol and mannitol improves the stability and effectiveness of the preparation, reduces usage, and further reduces the environmental burden. Natural ingredients such as vitamin E, gingerol extract, oregano essential oil, and cinnamon essential oil not only have disease resistance, but also have certain insect repellent and antibacterial effects, reducing the use of pesticides. This eco-friendly design concept not only meets the needs of modern agricultural production, but also provides strong support for the sustainable development of agriculture, achieving a win-win situation for disease control and environmental protection.

[0076] It should be noted that, in this document, relational terms such as first and second, etc., are used only to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship or order between these entities or operations. Moreover, the term "comprises" or any other variant thereof is intended to cover non-exclusive inclusion, so that a process, method, article or device that includes a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device. In the absence of further limitations, an element defined by the sentence "comprises a ..." does not exclude the presence of other identical elements in the process, method, article or device that includes the element.

[0077] The above description is intended to enable one skilled in the art to implement or use the present invention. Various modifications to these embodiments will be readily apparent to one skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention is not limited to the embodiments shown herein but is intended to conform to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. An eco-friendly formulation for inducing plant systemic resistance to control pepper anthracnose, characterized by: The eco-friendly preparation comprises: potassium glutamate: 10-20 parts by weight; S-allyl-L-cysteine: 8-15 parts by weight; 2-piperidinic acid: 5-10 parts by weight; sodium lauryl sulfate: 3-6 parts by weight; citric acid: 1-3 parts by weight; polyethylene glycol: 3-6 parts by weight; copper sulfate: 0.3-0.8 parts by weight; diatomaceous earth: 6-10 parts by weight; seaweed extract: 8-12 parts by weight; chitosan: 4-7 parts by weight; sodium iron EDTA: 0.5-1.5 parts by weight; vitamin E: 0.5-1.5 parts by weight; mannitol: 2-5 parts by weight; gingerol extract: 3-6 parts by weight; oregano essential oil: 1-3 parts by weight; and cinnamon essential oil: 1-3 parts by weight.

2. The eco-friendly formulation for inducing plant systemic resistance to control pepper anthracnose according to claim 1, characterized in that: The preparation method of the preparation comprises the following steps: S1: Weigh potassium glutamate, S-allyl-L-cysteine, 2-piperidinic acid, sodium lauryl sulfate, citric acid, polyethylene glycol, copper sulfate, diatomaceous earth, seaweed extract, chitosan, sodium iron EDTA, vitamin E, mannitol, gingerol extract, oregano essential oil and cinnamon essential oil according to the weight parts range in the above formula for later use; S2: Weighed solid ingredients: potassium glutamate, S-allyl-L-cysteine, 2-piperidinic acid, sodium lauryl sulfate, citric acid, polyethylene glycol, copper sulfate, diatomaceous earth, seaweed extract, chitosan, sodium iron EDTA, vitamin E, mannitol, and gingerol extract are placed in a mixer and mixed evenly; S3: Slowly add the liquid ingredients: oregano essential oil and cinnamon essential oil to the solid ingredients in the blender while continuously stirring to ensure uniform mixing; S4: Continue stirring the mixed solid and liquid components until a uniform suspension or solution is formed; S5: After the mixture is cooled to room temperature, gently stir; S6: diluting the mixed solution to the desired concentration according to actual use requirements; S7: Perform quality checks on the diluted preparation, including pH value, viscosity, and suspension rate, to ensure that the preparation meets the requirements; S8: Place qualified products into appropriate containers and seal them for storage; S9: Mark the product name, ingredients, instructions for use, storage conditions, production date and expiration date on the product packaging.

3. The eco-friendly formulation for inducing plant systemic resistance to control pepper anthracnose according to claim 1, characterized in that: In step S1, an electronic balance with an accuracy of 0.01 g is used to accurately weigh the ingredients in strict accordance with the weight range specified in the recipe. After each ingredient is weighed, it is immediately transferred to a clean, dry container to avoid moisture absorption or cross contamination. All weighing operations are performed in an environment with a room temperature of 20-25° C. and a relative humidity of less than 50%.

4. The eco-friendly formulation for inducing plant systemic resistance to control pepper anthracnose according to claim 1, characterized in that: In step S2, the speed of the high-speed stirrer is set to 250 rpm, and the mixing process lasts for 15 minutes to ensure that all solid components are completely dispersed and mixed evenly; after the mixing is completed, the wall of the mixing container is thoroughly cleaned with a scraper to ensure that there is no residue.

5. The eco-friendly formulation for inducing plant systemic resistance to control pepper anthracnose according to claim 1, characterized in that: In step S3, during the injection process, the stirring speed is reduced to 150 rpm to ensure that the liquid component and the solid component are fully integrated and local aggregation is avoided; after the injection is completed, stirring is continued for 10 minutes to ensure that the mixture is uniform.

6. The eco-friendly formulation for inducing plant systemic resistance to control pepper anthracnose according to claim 1, characterized in that: In step S4, homogenization is performed at a pressure of 50-60 bar for 5 minutes. After the homogenization is completed, the uniformity and stability of the mixture are checked to ensure that a stable suspension or solution is formed. If stratification or precipitation is found, the mixture needs to be homogenized again until it meets the requirements.

7. The eco-friendly formulation for inducing plant systemic resistance to control pepper anthracnose according to claim 1, characterized in that: In step S5, the mixture is naturally cooled at room temperature of 20-25° C. for 30 minutes until the temperature of the mixture drops below 25° C.; after cooling, a low-speed stirrer is used at a speed not exceeding 50 rpm.

8. The eco-friendly formulation for inducing plant systemic resistance to control pepper anthracnose according to claim 1, characterized in that: In step S6, deionized water is used for dilution, and water is slowly added to the mixture at a rate of 100 ml per minute using a peristaltic pump, while a low-speed stirrer is used at a speed of 100 rpm to continuously stir; The dilution multiple should be strictly in accordance with the instructions to ensure the concentration of the diluted preparation is accurate; In step S7, the pH value is measured using a pH meter to ensure that it is within the range of 5.0-7.0; Use a viscometer to measure viscosity to ensure that it meets design requirements; use a suspension rate meter to measure the suspension rate to ensure that it reaches more than 95%; all test items must meet the preset standards, and unqualified products will be discarded.

9. The eco-friendly formulation for inducing plant systemic resistance to control pepper anthracnose according to claim 1, characterized in that: In step S8, after filling is completed, the container is immediately sealed using an aluminum foil sealing machine to ensure that the container is well sealed and there is no leakage; the sealed container is visually inspected to ensure that there is no damage or contamination.

10. The eco-friendly formulation for inducing plant systemic resistance to control pepper anthracnose according to claim 1, characterized in that: In step S9, the packaged products are subjected to a final quality inspection, including appearance inspection, label inspection, and sealing inspection. The bottle and label are inspected for stains and damage using a magnifying glass at a magnification of 10 times. The bottle cap is inspected for leaks using a sealing tester. All inspection items must meet quality standards, and unqualified products are discarded. Quality inspection is carried out in a dedicated quality inspection area with the ambient temperature controlled at 23±2℃.

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