Lactobacillus plantarum selenium particles as well as preparation method and application thereof

Abstract

The invention belongs to the technical field of production and application of probiotic microorganism organic selenium, and particularly relates to lactobacillus plantarum selenium particles as well as a preparation method and application thereof. The lactobacillus plantarum selenium particles are prepared from lactobacillus plantarum selenium spray-dried powder, a granulation carrier and water. Lactobacillus plantarum selenium spray-dried powder in the lactobacillus plantarum selenium particles is prepared by producing lactobacillus plantarum through fermentation, converting sodium selenite into lactobacillus plantarum selenium fermentation liquor and carrying out spray drying, and can be widely used in the production process of agricultural products; the lactobacillus plantarum selenium organic selenium active component can effectively improve the oxidation resistance of animals and plants. The lactobacillus plantarum selenium granules are added into a crop root fertilizer or an animal feed to prepare a composite granular fertilizer or feed, so that the lactobacillus plantarum selenium granules can be effectively and passively absorbed and utilized by plants, the total organic selenium content in applied crop or animal tissues is increased, the disease resistance of crops or animals is effectively improved, and selenium-enriched agricultural products are produced.

Description

Technical Field

[0001] The invention belongs to the technical field of production and application of organic selenium from probiotic microorganisms, and particularly relates to Lactobacillus plantarum selenium particles, a preparation method and application thereof. Background Art

[0002] Selenium is an essential mineral for humans and animals, with physiological functions such as enhancing immunity, fighting cancer, and providing antioxidant benefits. Selenium was discovered in 1817 by the Swedish physicist Berzelius from a sticky substance at the bottom of a lead chamber in a sulfuric acid plant. Initially classified as a toxic substance, it was not recognized as an essential nutrient for animals until 1957.

[0003] Selenium, an essential trace element for the human body, is closely related to numerous bodily functions. It is a key component of glutathione peroxidase and 5-deiodinase, and plays a crucial role in antioxidant and stress resistance. Selenium deficiency can cause conditions such as muscular dystrophy, exudative diathesis, and pancreatic degeneration. Selenium supplementation can prevent and treat liver disease, enhance immunity, maintain overall health, and improve the quality of livestock and poultry products. Although selenium is present in very small amounts in the human body, it plays an irreplaceable role in human health, providing essential conditions for the survival of life. Selenium in the body requires continuous intake and is continuously maintained throughout bodily activity. Therefore, selenium intake and supplementation have become a symbol of human health.

[0004] 72% of my country's land area is selenium-deficient to varying degrees, and dietary selenium supplementation is now a common practice in my country's agricultural products. For a long time, inorganic selenium (such as sodium selenite and sodium selenate) has been added to livestock and poultry feed or fertilizer as a selenium source. However, due to sodium selenite's low bioavailability, high toxicity, and difficulty in controlling dosage, its irrational use has had adverse effects on animals and the environment. Bioconversion can convert toxic inorganic selenium into organic selenium, reducing its toxic side effects and increasing its physiological activity and absorption rate.

[0005] The present invention is based on the principle of biotransformation, utilizes probiotic plant lactobacillus (purchased from China Industrial Microorganism Culture Collection Administration Center, brand CICC 20263) to convert sodium selenite into plant lactobacillus selenium fermentation liquid of organic selenium, adds carrier, and is made into granules through spray drying. Plant lactobacillus selenium granules are a kind of organic selenium-rich additive, can play the dual role of organic selenium and probiotics. Compared with inorganic selenium, plant lactobacillus selenium granules are non-toxic and harmless, and have high bioavailability. Summary of the Invention

[0006] The purpose of the present invention is to address the problems in the prior art that inorganic selenium or organic selenium has low utilization rate and high toxicity during the production process, has adverse effects on environmental pollution, and cannot be safely utilized in crops or farmed animals, and to propose a Lactobacillus plantarum selenium particle and its preparation method and application.

[0007] The Lactobacillus plantarum selenium spray-dried powder in the Lactobacillus plantarum selenium granules of the present invention is prepared by fermenting Lactobacillus plantarum (strain preservation number is CICC.20263) to convert sodium selenite into Lactobacillus plantarum selenium through spray drying.

[0008] In the plant lactobacillus selenium granules prepared by the present invention, active ingredient organic selenium can effectively improve the antioxidant capacity of animals and plants, and can be widely used in agricultural product production processes. By adding the plant lactobacillus selenium granules in crop root fertilizers or in economic animal feeds, and making composite granular fertilizers or feeds, organic selenium therein can be passively and effectively absorbed and utilized by plants, improves the total organic selenium content in crops or animal tissues, and then effectively improves the disease resistance of crops or animals. The plant lactobacillus selenium granules of the present invention are conducive to producing selenium-rich agricultural products, increase the added value of agricultural products, and have good application prospects and economic value.

[0009] To achieve the above object, the technical solution created by the present invention is implemented as follows:

[0010] The invention discloses a Lactobacillus plantarum selenium granule, which is prepared from Lactobacillus plantarum selenium spray-dried powder, a granulation carrier and water.

[0011] Specifically, the Lactobacillus plantarum selenium spray-dried powder is prepared by adding a spray drying carrier to a Lactobacillus plantarum selenium fermentation liquid and performing high-temperature spray drying.

[0012] The Lactobacillus plantarum selenium fermentation liquid is obtained by converting sodium selenite by fermenting Lactobacillus plantarum (brand CICC 20263).

[0013] Preferably, the spray drying carrier is any one of maltodextrin and Jiayi powder, or a combination of two.

[0014] Specifically, the Lactobacillus plantarum selenium granules are prepared by first adding a granulation carrier and water to Lactobacillus plantarum selenium spray-dried powder, then mixing the mixture evenly, and then granulating and drying the mixture.

[0015] Preferably, the granulation carrier is any one or more combinations of zeolite powder, bentonite powder, sucrose powder, glucose powder, maltodextrin, and Jiayi powder.

[0016] Further preferably, in the Lactobacillus plantarum selenium granules, the mass ratio between the Lactobacillus plantarum selenium spray-dried powder and the granulation carrier is 1:(3-200).

[0017] Further preferably, the granulation carrier is a composite granulation carrier composed of zeolite powder and bentonite powder, and the mass ratio of the two is 1:2 to 2:1; or, the granulation carrier is a composite granulation carrier composed of zeolite powder, bentonite powder and sucrose powder; or, the granulation carrier is a composite granulation carrier composed of zeolite powder, bentonite powder and glucose powder; or, the granulation carrier is a composite granulation carrier composed of zeolite powder, bentonite powder, Jiayi powder and maltodextrin.

[0018] Furthermore, the present invention also provides a method for preparing Lactobacillus plantarum selenium particles, comprising the following steps:

[0019] (1) Preparation of Lactobacillus plantarum selenium spray-dried powder: Lactobacillus plantarum strains are activated → cultured in a shake flask → cultured in a first-level seed tank → cultured in a second-level seed tank → cultured in a third-level seed tank → cultured in a fourth-level fermentation tank with sodium selenite until the sodium selenite is converted into Lactobacillus plantarum selenium fermentation liquid → centrifuged, concentrated, and compounded with a spray drying carrier, and then spray-dried to prepare a spray-dried powder;

[0020] (2) Preparation of granulated Lactobacillus plantarum selenium composite powder: mixing the Lactobacillus plantarum selenium spray-dried powder of step (1) with a granulation carrier, and mixing the mixture in a mixer for 10 to 15 minutes to prepare the granulated Lactobacillus plantarum selenium composite powder;

[0021] (3) Preparation of Lactobacillus plantarum selenium granules: mixing the granulated Lactobacillus plantarum selenium composite powder of step (2) with water, stirring until the composite powder becomes a clumping wet powder, and then stirring and mixing for 10 to 15 minutes to obtain Lactobacillus plantarum selenium composite wet powder;

[0022] The prepared plant lactobacillus selenium composite wet powder is then added to the silo of a granulator to be made into plant lactobacillus selenium wet granules with different particle sizes and contents according to production requirements, and then dried until the moisture content is less than 6%.

[0023] Preferably, in step (1), the spray drying carrier is any one of maltodextrin and Jiayi powder, or a combination of two.

[0024] Specifically, in step (1), the fermentation conditions of the plant lactobacillus selenium fermentation liquid are as follows: fermentation production at a temperature of 20° C. to 30° C. under anaerobic conditions. The concentration of the sodium selenite solution used for conversion is 0.1 mmol / L to 10 mmol / L in terms of selenium, and the glucose concentration (mass percentage) used during the fermentation in the four-stage fermentation tank is 1% to 20%. All of the steps are sterilized in a feed tank at 121° C. to 125° C. for 40 to 60 minutes, and closed-loop feeding is performed during the fermentation in the four-stage fermentation tank according to the sodium selenite conversion detection value.

[0025] Specifically, in step (2), the granulation carrier is any one or more combinations of zeolite powder, bentonite powder, sucrose powder, glucose powder, maltodextrin, and Jiayi powder.

[0026] Specifically, in the Lactobacillus plantarum selenium granules described in step (2), the mass ratio between the Lactobacillus plantarum selenium spray-dried powder and the granulation carrier is 1:(3-200), preferably 1:(20-150).

[0027] Specifically, in step (3), when the plant lactobacillus selenium composite powder is granulated, the mass ratio of the plant lactobacillus selenium composite powder to water is 100:(20-55), and the preferred mixing ratio is 100:(35-45).

[0028] Furthermore, based on a general inventive concept, the present invention also provides the use of the Lactobacillus plantarum selenium particles in crops, fruits, vegetable fertilizers or animal feed.

[0029] Specifically, when used, adding the Lactobacillus plantarum selenium particles to crops, fruits, and vegetable fertilizers can increase the organic selenium content in plant and fruit agricultural products.

[0030] The amount used in agricultural products is:

[0031] 1) For field crops, calculate the amount of selenium by accurately weighing Lactobacillus plantarum selenium granules, using a dosage of 1.0-2.0g pure selenium / mu;

[0032] 2) For fruits and vegetables, calculate the amount of selenium and accurately weigh the Lactobacillus plantarum selenium granules. The usage amount is 3.0-5.0g pure selenium / mu.

[0033] 3) applying the Lactobacillus plantarum selenium particles from step 1) or 2) alone or mixed with other fertilizers around the roots of crops or fruits and vegetables.

[0034] Specifically, in step 1), the agricultural product is a food crop such as corn, wheat or sorghum. The amount of Lactobacillus plantarum selenium particles used is 1-2 g pure selenium per mu (calculated as pure selenium).

[0035] Preferably, in step 1), the usage of the Lactobacillus plantarum selenium particles is 1 g of selenium per mu calculated as pure selenium.

[0036] Specifically, in step 2), the agricultural products are cash crops such as melons, fruits, and vegetables. The amount of Lactobacillus plantarum selenium granules used is 2-5 g pure selenium per mu (calculated as pure selenium);

[0037] Preferably, in step 2), the usage of the Lactobacillus plantarum selenium particles is 3 g selenium / mu calculated as pure selenium.

[0038] Specifically, in step 3), the Lactobacillus plantarum selenium particles are used alone or mixed with other fertilizers to fertilize around the roots of crops or fruits and vegetables.

[0039] Specifically, when used, adding the Lactobacillus plantarum selenium particles to animal feed can increase the organic selenium content of economic animal agricultural products.

[0040] The dosage in animal breeding is:

[0041] 1) Calculate the amount of selenium by accurately weighing Lactobacillus plantarum selenium granules, using an amount of 0.5-2.0g pure selenium per ton of feed;

[0042] 2) Evenly mixing the Lactobacillus plantarum selenium particles prepared in step 1) with feed, and feeding the mixture to farmed animals.

[0043] Specifically, in step 1), the animals are economic animals such as pigs, chickens, ducks, geese, and cattle. The amount of Lactobacillus plantarum selenium particles used is 0.5 to 2.0 g of pure selenium per ton of feed.

[0044] Preferably, in step 1), the usage of the Lactobacillus plantarum selenium particles in meat-producing economic animals is 1.0 g of pure selenium per ton of feed, calculated as pure selenium.

[0045] Further preferably, in step 2), the usage of the Lactobacillus plantarum selenium particles in laying fowl and economic animals is 1.5 g pure selenium per ton of feed calculated as pure selenium.

[0046] Furthermore, based on a general inventive concept, the present invention also provides a selenium-rich fertilizer for crops or a selenium-rich feed for animal breeding prepared using the Lactobacillus plantarum selenium particles.

[0047] Specifically, the present invention also provides the use of the selenium-rich fertilizer for crops or the selenium-rich feed for animal breeding in the production of selenium-rich agricultural products.

[0048] Specifically, the present invention also provides the use of the selenium-rich fertilizer for crops or the selenium-rich feed for animal breeding in improving the disease resistance of crops and the healthy growth of livestock and poultry such as economic animals.

[0049] The selenium-enriched crop fertilizer of the present invention can be applied to crops to produce organic selenium-enriched grains, melons, fruits and vegetables, thereby increasing the organic selenium enrichment in the crop products.

[0050] The invention provides the economic animals with the selenium-rich drinking water for animal breeding, thereby enhancing the disease resistance of the animals, improving their health and increasing the organic selenium enrichment in the animal products.

[0051] The advantages of the Lactobacillus plantarum selenium-enriched particles of the present invention are that they can fully utilize crops or economic animal breeding to produce low-cost selenium-enriched agricultural products for people's daily consumption, including the following aspects:

[0052] (1) Celery contains 0.0781 mg / Kg of organic selenium, which meets the standard of selenium-rich vegetables (0.01 mg / Kg to 0.10 mg / Kg).

[0053] (2) The organic selenium content of Millennium Fruit is 0.0622 mg / Kg, which meets the standard of selenium-rich fruit (0.01 mg / Kg ~ 0.10 mg / Kg).

[0054] (3) The amount of organic selenium in eggs produced by laying hens is 0.5564 mg / Kg, which can meet the standard of selenium-rich eggs (0.15 mg / Kg ~ 0.50 mg / Kg).

[0055] (4) The amount of organic selenium in the chicken produced by broiler chickens is 0.1756 mg / Kg, which can meet the standard of selenium-rich fresh meat (0.02 mg / Kg ~ 0.50 mg / Kg).

[0056] (5) The amount of organic selenium in fresh milk produced by dairy cows is 0.0519 mg / Kg, which can meet the standard of selenium-rich fresh milk (0.01 mg / Kg~0.10 mg / Kg).

[0057] Compared with the prior art, the present invention has the following beneficial effects:

[0058] 1. After testing, the agricultural products produced using the plant lactobacillus selenium particles of the present invention can meet the selenium-rich agricultural product standards in terms of selenium-rich agricultural product indicators, and have good application value and market promotion prospects in crop planting and production and economic animal breeding industries.

[0059] 2. Selenium-enriched fertilizers for crops or selenium-enriched feed for animal husbandry produced using the plant lactobacillus selenium granules of the present invention can improve the disease resistance of crops and the healthy growth of livestock and poultry such as cash animals. Fertilizing crops with the selenium-enriched fertilizers of the present invention can produce grains, melons, fruits, and vegetables enriched with organic selenium, thereby increasing the organic selenium content in the crop products. Giving the selenium-enriched drinking water for animal husbandry described in the present invention to cash animals can enhance the animals' disease resistance, improve their health, and increase the organic selenium content in the animal products. BRIEF DESCRIPTION OF THE DRAWINGS

[0060] Figure 1 This is a flow chart of the production process of Lactobacillus plantarum selenium particles in Example 1;

[0061] Figure 2 The total selenium detection data and spectrum in Example 2, wherein the upper figure is the total selenium detection data, and the lower figure is the total selenium detection spectrum;

[0062] Figure 3 The inorganic selenium detection data and spectrum in Example 2, wherein the upper figure is the inorganic selenium detection data, and the lower figure is the inorganic selenium detection spectrum;

[0063] Figure 4 This is a photo of Lactobacillus plantarum selenium wet granules of different particle sizes prepared in Example 3;

[0064] Figure 5 The total selenium detection data and spectrum in Example 3, wherein the upper figure is the total selenium detection data, and the lower figure is the total selenium detection spectrum;

[0065] Figure 6 This is a photo of Lactobacillus plantarum selenium wet granules of different particle sizes prepared in Example 4;

[0066] Figure 7 The total selenium detection data and spectrum in Example 4, wherein the upper figure is the total selenium detection data, and the lower figure is the total selenium detection spectrum;

[0067] Figure 8 These are photos of Lactobacillus plantarum selenium wet granules of different particle sizes prepared in Example 5;

[0068] Figure 9 The total selenium detection data and spectrum in Example 5, wherein the upper figure is the total selenium detection data, and the lower figure is the total selenium detection spectrum;

[0069] Figure 10 Photos of Lactobacillus plantarum selenium wet granules of different particle sizes prepared in Example 6;

[0070] Figure 11 The total selenium detection data and spectrum in Example 6, wherein the upper figure is the total selenium detection data, and the lower figure is the total selenium detection spectrum;

[0071] Figure 12 The total selenium detection data and graph of selenium-enriched celery in Example 7, wherein the upper graph is the total selenium detection data, and the lower graph is the total selenium detection graph;

[0072] Figure 13 The total selenium detection data and spectrum of the selenium-rich Millennium Fruit in Example 7, wherein the upper figure is the total selenium detection data, and the lower figure is the total selenium detection spectrum;

[0073] Figure 14 The total selenium detection data and graph of the selenium-enriched egg product in Example 8, wherein the upper graph is the total selenium detection data, and the lower graph is the total selenium detection graph;

[0074] Figure 15 The total selenium detection data and graph of the selenium-enriched chicken product in Example 9, wherein the upper graph is the total selenium detection data, and the lower graph is the total selenium detection graph;

[0075] Figure 16 These are the total selenium detection data and graph of the selenium-enriched fresh milk product in Example 10, where the upper graph is the total selenium detection data and the lower graph is the total selenium detection graph. DETAILED DESCRIPTION

[0076] In order to better understand the present invention, the present invention is further described below in conjunction with the examples. However, the content of the present invention is not limited to the following examples. Any changes or equivalent substitutions that do not deviate from the concept of the present invention are included in the protection scope of the present invention.

[0077] The raw materials and equipment described in the present invention can be selected from products sold in the market. The Lactobacillus plantarum (brand number CICC.20263) used in the present invention was purchased from the China Industrial Culture Collection Center (CICC).

[0078] Example 1 Production process of Lactobacillus plantarum selenium granules

[0079] The conventional method in the prior art is adopted. The process flow of the specific invention embodiment 1 is shown in Figure 1 The fermentation culture, spray drying powder preparation and selenium wet granule preparation methods and steps not described in this embodiment all adopt conventional means in the prior art. The production process of the Lactobacillus plantarum selenium granules includes the following steps:

[0080] The Lactobacillus plantarum (strain number CICC.20263) is first melted and activated at room temperature → cultured in a shake flask → cultured in a first-level seed tank → cultured in a second-level seed tank → cultured in a third-level seed tank → cultured in a fourth-level fermentation tank with sodium selenite until the sodium selenite is converted into Lactobacillus plantarum selenium fermentation liquid → centrifuged and concentrated to obtain a concentrated liquid, which is then compounded with a spray drying carrier to obtain a spray-dried raw powder.

[0081] The spray drying carrier is any one of maltodextrin and Jiayi powder or a combination of the two.

[0082] In the spray-dried raw powder, the ratio (mass ratio) of the spray-dried carrier to the spray-fermented concentrated liquid is 1‰ to 10‰, preferably 1‰ to 2‰.

[0083] The fermentation conditions of the Lactobacillus plantarum selenium fermentation broth are as follows: fermentation at a temperature of 20°C to 30°C under anaerobic conditions. The concentration of the sodium selenite solution used for conversion is 0.1mmol / L to 10mmol / L in terms of selenium, and the glucose concentration (mass percentage) used during fermentation in the four-stage fermenter is 1% to 20%. Both are prepared by high-temperature sterilization at 121°C to 125°C for 40 to 60 minutes in a feeding tank. During the fermentation in the four-stage fermenter, closed-loop feeding is performed based on the sodium selenite conversion test value.

[0084] Then, the produced spray-dried raw powder is spray-dried to prepare spray-dried powder, and after being fully and evenly mixed with a granulation carrier, water is added and stirred evenly to form a wet powder, which is then granulated on a granulator. The prepared wet granules are dried at 50-60° C. until the moisture content is less than 6%, thereby obtaining plant lactobacillus selenium granules, which are then packaged and sealed.

[0085] Example 2

[0086] Example 2 provides a method for detecting the performance of the Lactobacillus plantarum selenium spray-dried powder produced in Example 1.

[0087] (1) Detection of total selenium in Lactobacillus plantarum selenium spray-dried powder

[0088] (1) Experimental materials: HNO3, HClO4, HCl, NaOH, and NaBH4 are all of high-grade purity; experimental equipment: 100 mL tall beaker, 50 mL colorimetric tube, 50 mL volumetric flask, watch glass, hot plate, analytical balance, atomic fluorescence spectrometer, etc.

[0089] (2) Reagent configuration:

[0090] Sodium borohydride alkaline solution (20 g / L): Weigh 20 g of sodium borohydride and 5 g of sodium hydroxide, dissolve in 1000 mL of water, and mix well.

[0091] Hydrochloric acid solution (20%): Measure 200 mL of hydrochloric acid and slowly add it to 500 mL of water. After cooling, dilute to 1000 mL with purified water and mix well.

[0092] Nitric acid-perchloric acid mixed acid (9+1): Mix 900 mL of nitric acid with 100 mL of perchloric acid.

[0093] Hydrochloric acid solution (6 mol / L): Measure 500 mL of hydrochloric acid and slowly add it to 400 mL of water. After cooling, dilute to 1000 mL with purified water and mix well.

[0094] Potassium ferrocyanide solution (100 g / L): Weigh 50 g of potassium ferrocyanide, dissolve it in 500 mL of water, and mix well.

[0095] Sample dilution of Lactobacillus plantarum selenium spray-dried powder: Measure 100 mL of hydrochloric acid and 50 mL of potassium ferricyanide solution (100 g / L), slowly add them into 200 mL of water, cool and dilute to 500 mL with purified water, and mix well.

[0096] (3) Preparation of standard solution

[0097] Selenium Standard Solution: Pipette 0 mL, 0.100 mL, 0.200 mL, 0.400 mL, and 1 mL of a 10 mg / L selenium standard solution into a 100 mL volumetric flask. Add 10 mL of 100 g / L potassium ferricyanide solution. Bring the volume to the mark with 20% hydrochloric acid solution and mix thoroughly before testing. The mass concentrations of this selenium standard series are 0 μg / L, 10 μg / L, 20 μg / L, 40 μg / L, and 100 μg / L, respectively.

[0098] (4) Preparation of Lactobacillus plantarum selenium spray-dried powder test sample

[0099] Weigh about 0.5 g of the produced Lactobacillus plantarum selenium spray-dried powder (accurate to 0.0001 g), place it in a 100 mL tall beaker, add 10 mL of nitric acid-perchloric acid mixture (9:1), cover with a watch glass, and cold digest overnight.

[0100] Next day on electric hot plate, heat, and in time add nitric acid process to sample solution for translucent, volume is about 2mL, cool, add 6mL hydrochloric acid solution (6mol / L) again, continue to be heated to clear and colorless and with white smoke to occur.After cooling, transfer in the 50mL colorimetric tube, and rinse beaker 3-5 time with purified water, move in the colorimetric tube in the lump, add 10mL potassium ferricyanide solution (100g / L), use purified water constant volume, mix.According to sample concentration to constant volume rear sample, carry out gradient dilution with plant lactobacillus selenium spray-dried powder sample diluent, make diluted back sample concentration in 10-100 μ g / L.To be measured, do blank experiment simultaneously, blank dilution multiple is identical with sample.

[0101] (2) Detection of inorganic selenium content in Lactobacillus plantarum selenium spray-dried powder

[0102] Get about 0.5-1g plant lactobacillus selenium spray-dried powder sample (being accurate to 0.0001g), be placed in 50mL volumetric flask, add about 25mL water, ultrasonic dissolution 10min, fully dissolves plant lactobacillus selenium spray-dried powder, adds purified water and is settled to scale, shakes up.After constant volume, solution is placed in 8000r / min centrifuge, centrifugal 5min, gets centrifugal sample and carries out gradient dilution (making dilution rear sample concentration in 10-100 μ g / L) to be measured, does blank test simultaneously.

[0103] (III) Calculation of organic selenium content in Lactobacillus plantarum selenium spray-dried powder

[0104] Organic selenium content = total selenium content - inorganic selenium content;

[0105]

[0106] (IV) Test results of total selenium, inorganic selenium and organic selenium in Lactobacillus plantarum selenium spray-dried powder

[0107] For quality inspection data and graphs of total selenium, please refer to Figure 2 For the detection data and spectrum of inorganic selenium, please refer to Figure 3 .

[0108] Percentage of organic selenium:

[0109]

[0110] It can be seen from the above sample test results that the organic selenium content in the Lactobacillus plantarum selenium spray-dried powder produced by the present invention is 99.9655%, which can meet the production and application requirements.

[0111] Example 3

[0112] Example 3 provides a method for producing 1200 kg of 500 ppm Lactobacillus plantarum selenium particles, the specific steps being as follows:

[0113] (1) Preparation of granulation carrier:

[0114] Weigh 500 kg of zeolite powder and 690 kg of bentonite powder, mix them thoroughly in a mixer for 15 minutes, and prepare 1190 kg of composite granulation carrier for later use.

[0115] (2) Preparation of granulated Lactobacillus plantarum selenium composite powder:

[0116] Take 10 kg of the qualified plant lactobacillus selenium spray-dried powder tested in Example 2 and 1190 kg of the composite granulation carrier in step (1) (i.e., the mass ratio is 1:119) and fully mix them in a mixer for 15 minutes to prepare a granulated plant lactobacillus selenium composite powder for standby use.

[0117] (3) Preparation of plant lactobacillus selenium composite wet powder:

[0118] Weigh 1200 kg of the granulated plant lactobacillus selenium composite powder of step (2), add 420 kg of drinking water, and stir evenly while adding water until the composite powder becomes a wet powder that can be agglomerated by hand, and then fully stir and mix for 10 to 15 minutes to obtain the plant lactobacillus selenium composite wet powder, which is set aside.

[0119] (4) Preparation of 500ppm Lactobacillus plantarum selenium granules:

[0120] The plant lactobacillus selenium composite wet powder prepared in step (3) is added to the hopper of the granulator, and 500ppm plant lactobacillus selenium wet granules of different particle sizes (such as Figure 4 The prepared Lactobacillus plantarum selenium wet granules are dried at 50-60° C. until the moisture content is less than 6%, thereby obtaining 500 ppm Lactobacillus plantarum selenium granules, which are then packaged and sealed.

[0121] (5) The detection method of the 500ppm plant lactobacillus selenium granules of the present embodiment is as shown in Example 2. Figure 5 shown.

[0122] From the sample test results, it can be seen that the total selenium content in the produced 500ppm Lactobacillus plantarum selenium granules is 503.7595mg / Kg, which can meet the standard of 500ppm Lactobacillus plantarum selenium granules (≥500mg / Kg).

[0123] Example 4

[0124] Example 4 provides a method for producing 1800 kg of 1000 ppm Lactobacillus plantarum selenium particles. The preparation method in Example 4 differs from that in Example 3 in that steps (1), (2), and (3);

[0125] The step (1) is as follows: 800 kg of zeolite powder, 900 kg of bentonite powder and 70 kg of sucrose powder are weighed and fully mixed in a mixer for 15 minutes to prepare 1770 kg of a composite granulation carrier for standby use.

[0126] Step (2) is: taking 30 kg of the qualified plant lactobacillus selenium spray-dried powder detected in Example 2 and 1770 kg of the composite granulation carrier in step (1) (i.e., the mass ratio is 1:59), and fully mixing them in a mixer for 15 minutes to prepare a granulated plant lactobacillus selenium composite powder for standby use.

[0127] Step (3) comprises the following steps: weighing 1800 kg of the granulated plant lactobacillus selenium composite powder prepared in step (2), adding 684 kg of drinking water, stirring evenly while adding water, until the composite powder becomes a wet powder that can be agglomerated by hand, and then stirring and mixing for 10 to 15 minutes to obtain the plant lactobacillus selenium composite wet powder for standby use.

[0128] 1000ppm plant lactobacillus selenium wet granules of different particle sizes prepared in Example 4 (such as Figure 6 The test results of the finished product of Example 4 are as shown. Figure 7 shown.

[0129] From the sample test results, it can be seen that the total selenium content in the produced 1000ppm Lactobacillus plantarum selenium granules is 1043.4177mg / Kg, which can meet the standard of 1000ppm Lactobacillus plantarum selenium granules (≥1000mg / Kg).

[0130] Example 5

[0131] Example 5 provides a method for producing 1500 kg of 2000 ppm Lactobacillus plantarum selenium particles. The preparation method in Example 5 differs from that in Example 3 in that steps (1), (2), and (3);

[0132] The step (1) is as follows: 600 kg of zeolite powder, 800 kg of bentonite powder and 50 kg of glucose powder are weighed and fully mixed in a mixer for 15 minutes to prepare 1450 kg of a composite granulation carrier for standby use.

[0133] Step (2) is: taking 50 kg of the qualified plant lactobacillus selenium spray-dried powder detected in Example 2 and 1450 kg of the composite granulation carrier in step (1) (i.e., the mass ratio is 1:29), and fully mixing them in a mixer for 15 minutes to prepare a granulated plant lactobacillus selenium composite powder for standby use.

[0134] Step (3) comprises the following steps: weighing 1500 kg of the granulated plant lactobacillus selenium composite powder prepared in step (2), adding 540 kg of drinking water, stirring evenly while adding water, until the composite powder becomes a wet powder that can be agglomerated by hand, and then stirring and mixing for 10 to 15 minutes to obtain the plant lactobacillus selenium composite wet powder for standby use.

[0135] 2000ppm plant lactobacillus selenium wet granules of different particle sizes prepared in Example 5 (such as Figure 8 The test results of the finished product of Example 5 are as shown. Figure 9 shown.

[0136] It can be seen from the sample test results that the total selenium content in the produced 2000ppm Lactobacillus plantarum selenium granules is 2036.8035mg / Kg, which can meet the standard of 2000ppm Lactobacillus plantarum selenium granules (≥2000mg / Kg).

[0137] Example 6

[0138] Example 6 provides a method for producing 1000 kg of 3000 ppm Lactobacillus plantarum selenium particles. The preparation method in Example 6 differs from that in Example 3 in that steps (1), (2), and (3);

[0139] The step (1) is as follows: 400 kg of zeolite powder, 500 kg of bentonite powder, 25 kg of Jiayi powder and 25 kg of maltodextrin are weighed and fully mixed in a mixer for 15 minutes to prepare 950 kg of composite granulation carrier for standby use.

[0140] Step (2) is: taking 50 kg of the qualified plant lactobacillus selenium spray-dried powder detected in Example 2 and 950 kg of the composite granulation carrier in step (1) (i.e., the mass ratio is 1:19), and fully mixing them in a mixer for 15 minutes to prepare a granulated plant lactobacillus selenium composite powder for standby use.

[0141] Step (3) comprises the following steps: weighing 1000 kg of the granulated plant lactobacillus selenium composite powder prepared in step (2), adding 400 kg of drinking water, stirring evenly while adding water, until the composite powder becomes a wet powder that can be agglomerated by hand, and then stirring and mixing for 10 to 15 minutes to obtain the plant lactobacillus selenium composite wet powder, which is then set aside.

[0142] 3000ppm plant lactobacillus selenium wet granules of different particle sizes prepared in Example 6 (such as Figure 10 The test results of the finished product of Example 5 are as shown. Figure 11 shown.

[0143] From the sample test results, it can be seen that the total selenium content in the produced 3000ppm Lactobacillus plantarum selenium granules is 3033.9817mg / Kg, which can meet the standard of 3000ppm Lactobacillus plantarum selenium granules (≥3000mg / Kg).

[0144] Example 7

[0145] Example 7 provides the application of the Lactobacillus plantarum selenium particles in producing selenium-enriched vegetables or selenium-enriched fruits, and the specific steps are as follows:

[0146] (1) Fertilization of fruits and vegetables is calculated with selenium. Accurately weigh the plant lactobacillus selenium granules in an amount of 3.0 to 5.0 g of pure selenium per mu. In this embodiment, 6 kg of a 500 ppm plant lactobacillus selenium granule product, 3 kg of a 1000 ppm plant lactobacillus selenium granule product, 1.5 kg of a 2000 ppm plant lactobacillus selenium granule product, or 1 kg of a 3000 ppm plant lactobacillus selenium granule product are taken.

[0147] (2) The plant lactobacillus selenium particles weighed in step (1) are applied alone or mixed with other fertilizers around the roots of celery or cherry tomato seedlings, irrigated and watered, and harvested when the agricultural products are mature.

[0148] (3) The detection method of selenium-rich agricultural products is as follows: Referring to Example 2, the detection results of the finished products are as follows.

[0149] The test results of selenium-rich celery (such as Figure 12 shown):

[0150] From the sample test results, it can be seen that the amount of organic selenium contained in selenium-rich celery is 0.0781 mg / Kg, which meets the standard of selenium-rich vegetables (0.01 mg / Kg ~ 0.10 mg / Kg).

[0151] The test results of selenium-rich millennium fruit (cherry tomatoes) (such as Figure 13 shown):

[0152] From the sample test results, it can be seen that the selenium-rich Millennium Fruit contains 0.0622 mg / Kg of organic selenium, which meets the standard of selenium-rich fruit (0.01 mg / Kg~0.10 mg / Kg).

[0153] Example 8

[0154] Example 8 provides the application of the Lactobacillus plantarum selenium particles in producing selenium-rich egg agricultural products in egg-laying poultry farming, and the specific steps are as follows:

[0155] (1) The feeding addition amount for poultry and egg farming is calculated with selenium. The plant lactobacillus selenium granules are accurately weighed, and the usage amount is 0.5-2.0g pure selenium / feed. In this embodiment, 2kg of 500ppm plant lactobacillus selenium granule product, 1kg of 1000ppm plant lactobacillus selenium granule product, 0.5kg of 2000ppm plant lactobacillus selenium granule product, or 0.35kg of 3000ppm plant lactobacillus selenium granule product are taken.

[0156] (2) Evenly mix the plant lactobacillus selenium particles of step (1) with feed, and feed the laying hens for 30 days or more.

[0157] (3) The detection method of selenium-rich egg products is as follows (see Example 2). Figure 14 shown).

[0158] From the sample test results, it can be seen that the amount of organic selenium in eggs produced by laying hens is 0.5564 mg / Kg, which can meet the standard of selenium-rich eggs (0.15 mg / Kg ~ 0.50 mg / Kg).

[0159] Example 9

[0160] Example 9 provides the application of the Lactobacillus plantarum selenium particles in producing selenium-rich chicken agricultural products in broiler farming, and the specific steps are as follows:

[0161] (1) Meat poultry breeding and feeding addition is calculated with selenium, and the plant lactobacillus selenium particles are accurately weighed, and the usage amount is 0.5-2.0g pure selenium / feed. In this embodiment, 2kg of 500ppm plant lactobacillus selenium particle product, or 1kg of 1000ppm plant lactobacillus selenium particle product, or 0.5kg of 2000ppm plant lactobacillus selenium particle product, or 0.35kg of 3000ppm plant lactobacillus selenium particle product are taken.

[0162] (2) Evenly mix the Lactobacillus plantarum selenium particles of step (1) with feed, and feed the broiler chickens for 35 days or more.

[0163] (3) The detection method of selenium-enriched chicken products is as follows (see Example 2). Figure 15 shown).

[0164] From the sample test results, it can be seen that the chicken produced by broiler chickens contains 0.1756 mg / Kg of organic selenium, which can meet the standard of selenium-rich fresh meat (0.02 mg / Kg~0.50 mg / Kg).

[0165] Example 10

[0166] Example 10 provides the application of the Lactobacillus plantarum selenium particles in producing selenium-rich fresh milk agricultural products in dairy cattle breeding, and the specific steps are as follows:

[0167] (1) The feed addition for dairy cow breeding is calculated with selenium, and the plant lactobacillus selenium particles are accurately weighed, and the usage amount is 0.5-2.0g pure selenium / feed. In this embodiment, 2kg of 500ppm plant lactobacillus selenium particle product, 1kg of 1000ppm plant lactobacillus selenium particle product, 0.5kg of 2000ppm plant lactobacillus selenium particle product, or 0.35kg of 3000ppm plant lactobacillus selenium particle product are taken.

[0168] (2) Evenly mix the Lactobacillus plantarum selenium particles of step (1) with feed, and feed the broiler chickens for 45 days or more.

[0169] (3) The detection method of selenium-enriched fresh milk product is as follows (see Example 2). Figure 16 shown).

[0170] From the sample test results, it can be seen that the amount of organic selenium in the fresh milk produced by dairy cows is 0.0519 mg / Kg, which can meet the standard of selenium-rich fresh milk (0.01 mg / Kg~0.10 mg / Kg).

Claims

1. A Lactobacillus plantarum selenium granule, characterized in that The invention is prepared from Lactobacillus plantarum selenium spray-dried powder, granulation carrier and water.

2. The plant lactobacillus selenium granules according to claim 1, wherein The Lactobacillus plantarum selenium spray-dried powder is prepared by adding a spray-drying carrier into Lactobacillus plantarum selenium fermentation liquid and subjecting the mixture to high-temperature spray drying.

3. The plant lactobacillus selenium granules according to claim 2, wherein The Lactobacillus plantarum selenium fermentation liquid is produced by converting sodium selenite into Lactobacillus plantarum through fermentation.

4. The plant lactobacillus selenium granules according to claim 2, characterized in that The spray drying carrier is any one of maltodextrin and Jiayi powder or a combination of the two.

5. The plant lactobacillus selenium granules according to claim 1, characterized in that The granulation carrier is any one or more combinations of zeolite powder, bentonite powder, sucrose powder, glucose powder, maltodextrin, and Jiayi powder; in the plant lactobacillus selenium granules, the mass ratio between the plant lactobacillus selenium spray-dried powder and the granulation carrier is 1:(3-200).

6. The method for preparing Lactobacillus plantarum selenium particles according to any one of claims 1 to 5, characterized in that: The following steps are involved: (1) Preparation of Lactobacillus plantarum selenium spray-dried powder: Lactobacillus plantarum strains are activated → cultured in a shake flask → cultured in a first-level seed tank → cultured in a second-level seed tank → cultured in a third-level seed tank → cultured in a fourth-level fermentation tank with sodium selenite until the sodium selenite is converted into Lactobacillus plantarum selenium fermentation liquid → centrifuged, concentrated, and compounded with a spray drying carrier, and then spray-dried to prepare a spray-dried powder; (2) Preparation of granulated Lactobacillus plantarum selenium composite powder: mixing the Lactobacillus plantarum selenium spray-dried powder of step (1) with a granulation carrier, and mixing for 10 to 15 minutes to prepare a granulated Lactobacillus plantarum selenium composite powder; (3) Preparation of Lactobacillus plantarum selenium granules: mixing the granulated Lactobacillus plantarum selenium composite powder of step (2) with water, stirring until the composite powder becomes a clumping wet powder, and then stirring and mixing for 10 to 15 minutes to obtain Lactobacillus plantarum selenium composite wet powder; The prepared plant lactobacillus selenium composite wet powder is then made into plant lactobacillus selenium wet granules with different particle sizes and contents, and then dried until the moisture content is less than 6%.

7. The preparation method according to claim 6, characterized in that In step (1), the plant lactobacillus selenium fermentation liquid is fermented and produced at a temperature of 20° C. to 30° C. under anaerobic conditions; the sodium selenite solution used for conversion has a concentration of 0.1 mmoL / L to 10 mmoL / L in terms of selenium, and a glucose concentration of 1% to 20%, and both are sterilized in a feed tank at 121° C. to 125° C. for 40 to 60 min.

8. The preparation method according to claim 6, characterized in that In step (2), the granulation carrier is any one or more combinations of zeolite powder, bentonite powder, sucrose powder, glucose powder, maltodextrin, and Jiayi powder; in the plant lactobacillus selenium granules described in step (2), the mass ratio between the plant lactobacillus selenium spray-dried powder and the granulation carrier is 1:(3-200).

9. The preparation method according to claim 6, characterized in that In step (3), when the plant lactobacillus selenium composite powder is granulated, the mass ratio of the plant lactobacillus selenium composite powder to water is 100: (20-55).

10. Use of the Lactobacillus plantarum selenium particles according to any one of claims 1 to 5 in crops, fruits, vegetable fertilizers or animal feed, characterized in that: Adding the plant lactobacillus selenium particles to crops, fruits, and vegetable fertilizers can increase the organic selenium content in plant and fruit agricultural products; Alternatively, adding the Lactobacillus plantarum selenium particles to animal feed can increase the organic selenium content of economic animal agricultural products.

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