An automated PCR anti-splash device

Abstract

The present application relates to an automated PCR anti-splash device, and relates to the field of medical instrument technology. An automated PCR anti-splash device includes a body and a base arranged at one end of the body, a sample pool for storing samples is arranged on the base, a cover is slidably arranged on the body, a heat cover is lifted and lowered inside the cover, and a sealing gasket for sealing the sample pool is arranged inside the heat cover; positioning rods are arranged at both ends of the length direction of the sample pool, and side panels are arranged on both sides of the width direction of the cover, and each of the side panels is provided with a limiting rod, the limiting rods are arranged at intervals along the length direction of the side panels, and each limiting rod abuts against the side wall of the positioning rod away from the base. The present application has the effect of reducing sample splashing when the heat cover is opened.

Description

Technical Field

[0001] The present application relates to the technical field of medical instruments, and in particular to an automated PCR anti-splash device. Background Art

[0002] An automated PCR instrument is a highly automated device that integrates PCR reaction, sample processing, data analysis and other functions. It can perform PCR amplification and analysis quickly and accurately without human intervention.

[0003] In the related art, an automated PCR includes a body and a base arranged at one end of the body, wherein a sample pool for storing samples is arranged on the base, a reaction module for catalyzing sample reactions is arranged inside the base, and the conical tube on the sample pool is inserted into the conical hole of the reaction module; a cover is slidably installed on the body along the length direction of the body by a translation mechanism, and a hot cover for covering the sample pool is installed inside the cover by a lifting mechanism along the height direction of the body, a sealing gasket for sealing the sample inside the sample pool is arranged inside the hot cover, and an electric heating element for heating the sample is arranged in the body; when in use, the translation mechanism drives the cover and the hot cover to move above the sample pool, the lifting mechanism drives the hot cover and the sealing gasket to press and seal the sample inside the sample pool, the reaction module catalyzes the sample to react, and the electric heating element heats the sample; after use, the lifting structure drives the hot cover and the sealing gasket away from the sample pool, and then the translation mechanism drives the hot cover away from the sample pool for reset.

[0004] Regarding the above-mentioned related technologies, the sealing gasket on the hot cover is heated during the process of pressing and sealing the sample pool, which can easily cause the sealing gasket to adhere tightly to the sample pool; when the hot cover moves up and away from the sample pool, it can easily drive the sample pool to move up, and then when the hot cover drives the sample pool to move horizontally, the translated sample pool and the reaction module interfere with each other, forcing the sample pool to separate from the sealing gasket, which can easily cause the sample pool to vibrate violently, causing the sample inside the sample pool to splash, causing contamination of the experimental instrument, so it needs to be improved. Summary of the Invention

[0005] In order to improve the problem that samples inside the sample pool are easily splashed when the hot cover is opened and reset, the present application provides an automated PCR anti-splash device.

[0006] The present application provides an automated PCR anti-splash device that adopts the following technical solutions:

[0007] An automated PCR anti-splash device comprises a body and a base arranged at one end of the body, wherein a sample pool for storing samples is provided on the base, a cover is slidably provided on the body, a heat cover is lifted and lowered inside the cover, and a sealing gasket for sealing the sample pool is provided inside the heat cover; positioning rods are provided at both ends of the sample pool in the longitudinal direction, and side panels are provided on both sides of the cover in the width direction, and each of the side panels is provided with a limiting rod, the limiting rods are arranged at intervals along the longitudinal direction of the side panel, and each limiting rod abuts against the side wall of the positioning rod away from the base.

[0008] By adopting the above technical solution, the limiting rod and the positioning rod abut against the side wall of the base to limit the sample pool from moving up and down with the thermal cover, and quickly separate the sample pool from the sealing gasket, thereby reducing the phenomenon of sample splashing caused by the sample pool moving with the thermal cover when the thermal cover is opened.

[0009] Preferably, each side plate is provided with a waist-shaped groove on the side wall facing the positioning rod for the limiting rod to be inserted into, and an adjustment block is provided on the side plate for sliding along the vertical direction inside the waist-shaped groove, and the adjustment block is connected to the limiting rod, and an adjustment bolt for adjusting the position of the adjustment block is passed through the side plate.

[0010] By adopting the above technical solution, the adjustment block drives the limit rod to move up and down inside the waist-shaped groove, and the adjustment bolt is used to fix the position of the adjustment block and the limit rod, thereby realizing the adjustment of the height position of the limit rod, thereby facilitating the limiting of sample pools of different heights and improving the practicality of the overall anti-splash device.

[0011] Preferably, a locking assembly is provided between the base and the sample cell for locking the base and the sample cell.

[0012] By adopting the above technical solution, the locking assembly locks the base and the sample pool, reducing the phenomenon of the sample pool moving up and down with the thermal cover, so that the sample pool and the raised sealing gasket are quickly separated, thereby reducing the phenomenon of sample splashing when the thermal cover is opened.

[0013] Preferably, the locking assembly includes a plug-in plate, a locking rod and a driving member; the plug-in plate is arranged on the side wall of the sample pool facing the base, the top wall of the base is provided with a plug-in groove for the plug-in plate to be inserted into, the base is provided with a locking groove connected to the plug-in groove, the locking rod is slidably arranged inside the locking groove, the driving member is provided on the base to drive the locking rod close to or away from the plug-in plate, and a locking hole for the locking rod to pass through is provided on the plug-in plate.

[0014] By adopting the above technical solution, the driving member drives the locking rod to approach and penetrate the plug-in board to quickly connect the sample pool to the base; the driving member drives the locking rod away from and disengages from the plug-in board to facilitate the removal and replacement of the sample pool on the base.

[0015] Preferably, the driving member includes a guide rod, an anti-slip block, an elastic member and a driving rod; the guide rod is passed through the inside of the base, one end of the guide rod is connected to the end of the locking rod away from the plug-in plate, and the other end of the guide rod is exposed from the base; the anti-slip block is arranged at the end of the guide rod away from the locking rod, and the elastic member is arranged between the anti-slip block and the base to drive the locking rod away from the plug-in plate by its own elastic force; the driving rod is arranged on the machine cover, and the driving rod can be abutted against the side wall of the anti-slip block away from the guide plate.

[0016] By adopting the above technical solution, the elastic member uses its own elastic force to drive the guide rod to drive the locking rod away from and disengage from the plug-in board, so that the sample pool on the base can be normally removed and replaced; when the machine cover drives the thermal cover gradually close to the sample pool to prepare for the experiment, the driving rod on the machine cover presses the anti-detachment block, so that the anti-detachment block drives the guide rod and the locking rod gradually close to the plug-in board, and the locking rod is used to penetrate the plug-in rod to achieve a quick connection between the sample pool and the base; the driving rod is driven by the moving machine cover, so that the sample pool and the base are locked and connected during the experiment, reducing the phenomenon that the lifting and moving thermal cover drives the sample pool to move; after the machine cover drives the thermal cover to separate from the sample pool, the elastic member drives the locking rod to separate from the plug-in board, so that the operator can easily remove and replace the sample pool.

[0017] Preferably, each of the side panels is provided with a splitting assembly for splitting the sealing gasket and the sample cell.

[0018] By adopting the above technical solution, the split assembly separates the sealing gasket and the sample pool, thereby reducing the phenomenon of the sealing gasket and the sample pool adhering when the hot cover is opened and reducing the phenomenon of sample splashing inside the sample pool.

[0019] Preferably, the splitting assembly includes a splitting plate, a sliding rod and a driving cylinder; the splitting plate is slidably arranged between the side plate and the sample pool, a plurality of exhaust holes are penetrated through the side wall of the splitting plate, and a guide surface is provided on the side wall of the splitting plate facing the sample pool for facilitating insertion between the sample pool and the sealing gasket; the sliding rod is arranged on the side wall of the splitting plate away from the sample pool, and the end of the sliding rod away from the splitting plate penetrates the side plate; the driving cylinder is arranged on the side plate to drive the splitting plate close to or away from the sample pool.

[0020] By adopting the above technical solution, before the hot cover is opened, the driving cylinder drives the split plate to gradually approach the sample pool. The split plate uses the guide surface to press between the sample pool and the sealing gasket, and allows the air between the sample pool and the sealing gasket to circulate to the outside through the exhaust hole, so that the air pressure between the sample pool and the sealing gasket is consistent with that of the outside, thereby quickly separating the sample pool and the sealing gasket from each other.

[0021] Preferably, the sealing gasket is provided with a plurality of vent holes, a sealing component for sealing all the vent holes is provided between the thermal cover and the sealing gasket, and a separating component is provided on the thermal cover for releasing the sealing component from sealing the vent holes.

[0022] By adopting the above technical solution, the sealing component seals the vent hole on the sealing gasket, ensuring that the sealing gasket stably seals the sample pool during the experiment; the separation component releases the sealing component from the vent hole, so that the air pressure between the sample pool and the sealing gasket is consistent with that outside before the hot cover is opened, thereby quickly separating the sample pool and the sealing gasket from each other.

[0023] Preferably, the closing assembly includes a closing plate, a closing head, a guide rod and a pressing piece; the closing plate is slidably arranged between the thermal cover and the sealing gasket, the closing head is arranged on the side wall of the closing plate facing the sealing gasket, the closing heads and the vents are respectively arranged in a one-to-one correspondence, and each of the closing heads is used to seal the corresponding vent; the guide rod is arranged on the side wall of the thermal cover facing the sealing gasket, and the guide rod passes through the closing plate; the pressing piece is arranged between the closing plate and the thermal cover to drive the closing plate to fit tightly against the sealing gasket through its own elastic force.

[0024] By adopting the above technical solution, the pressing member uses its own elastic force to drive the closing plate to move along the length direction of the guide rod, and the closing plate uses the closing head to block all the vents to achieve sealing of the vents on the sealing gasket.

[0025] Preferably, the separation assembly includes an extension rod and a separation rod; the extension rod is arranged on the sliding rod, and the separation rod is arranged on the side wall of the extension rod facing the closing plate, the side wall of the thermal cover is penetrated by a passage for the separation rod to pass through, and the end of the separation rod facing the closing plate is provided with a lifting inclined surface for lifting the closing plate away from the sealing gasket.

[0026] By adopting the above technical solution, before the hot cover is opened, in the process of driving the cylinder to drive the split plate and the sliding rod to gradually approach the sample pool, the extension rod and the separation rod are driven by the movement of the sliding rod, so that the separation rod drives the closing plate and the closing head to gradually move away from the sealing gasket by using the lifting inclined surface, thereby canceling the blockage of the vent hole. Therefore, before the hot cover is opened, the air pressure between the sample pool and the sealing gasket is consistent with that outside, thereby facilitating the separation of the sample pool and the sealing gasket.

[0027] In summary, this application includes at least one of the following beneficial technical effects:

[0028] By setting the limit rod and the positioning rod to abut against the side wall of the base, the sample pool is restricted from following the lifting and lowering movement of the thermal cover, and the sample pool and the sealing gasket are quickly separated, thereby reducing the phenomenon of sample splashing caused by the sample pool following the movement of the thermal cover when the thermal cover is opened;

[0029] The base and the sample cell are locked together by setting a locking assembly, which reduces the phenomenon of the sample cell moving up and down with the hot cover, allowing the sample cell to be quickly separated from the raised sealing gasket, thereby reducing the phenomenon of sample splashing when the hot cover is opened;

[0030] The sealing gasket and the sample pool are separated by setting a split component, which reduces the phenomenon of the sealing gasket and the sample pool adhering when the hot cover is opened, and reduces the phenomenon of sample splashing inside the sample pool. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1 This is a schematic structural diagram of an automated PCR anti-splash device according to an embodiment of the present application.

[0032] Figure 2 It is a cross-sectional diagram used to reflect the connection relationship between the body, cover and thermal cover.

[0033] Figure 3 It is a cross-sectional schematic diagram used to show the internal structure of the body.

[0034] Figure 4 It is used to reflect Figure 3 A magnified schematic diagram of the structure at center A.

[0035] Figure 5 It is a cross-sectional schematic diagram used to reflect the connection relationship between the base and the sample pool.

[0036] Figure 6 It is a cross-sectional diagram used to illustrate the connection relationship between the locking assembly, the base and the sample cell.

[0037] Figure 7 It is a cross-sectional diagram used to illustrate the connection relationship between the closing component and the sealing gasket.

[0038] Figure 8 It is a structural diagram used to reflect the connection relationship between split components and separated components.

[0039] Description of reference numerals:

[0040] 1. Body; 11. Base; 111. Plug-in slot; 112. Locking slot; 12. Sample cell; 121. Positioning rod; 13. Cover; 131. Translation rack; 14. Heat cover; 141. Passageway; 15. Sealing gasket; 151. Vent; 16. Side panel; 161. Limiting rod; 162. Waist groove; 163. Adjustment block; 164. Adjustment bolt; 2. Locking assembly; 21. Plug-in plate; 211. Locking hole; 22. Lock Stop rod; 23. Driving member; 231. Guide rod; 232. Anti-slip block; 233. Elastic member; 234. Driving rod; 3. Splitting assembly; 31. Splitting plate; 311. Exhaust hole; 312. Guide surface; 32. Sliding rod; 33. Driving cylinder; 4. Closing assembly; 41. Closing plate; 42. Closing head; 43. Guide rod; 44. Pressing member; 5. Separating assembly; 51. Extension rod; 52. Separating rod; 521. Lifting ramp. DETAILED DESCRIPTION

[0041] The following is combined with Figure 1-8 This application is described in further detail.

[0042] The embodiment of the present application discloses an automated PCR anti-splash device for reducing sample splashing when the hot cover is opened.

[0043] Reference Figure 1 and Figure 2 An automated PCR splash-proof device includes a body 1 and a base 11 mounted at one end of the body 1. A sample pool 12 is sleeved on the base 11 for storing samples. A cover 13 is slidably mounted on the top of the body 1 along the length of the body 1. In this embodiment, a translation screw driven by a motor is rotatably mounted inside the body 1. A translation frame 131 fixedly connected to the cover 13 is threadedly connected to the translation screw, driving the cover 13 to move along the direction of the body 1. A heat cover 14 is installed inside the cover 13 by a cylinder. A silicone sealing gasket 15 is glued to the inside of the heat cover 14 to cover and seal the sample pool 12.

[0044] Reference Figure 3 and Figure 4 The sample pool 12 has positioning rods 121 bent and formed at both ends of its length. Side panels 16 are fixedly connected to both sides of the cover 13 along its width, with the sample pool 12 and the positioning rods 121 located between two sets of side panels 16. Each set of side panels 16 is mounted with several sets of limiting rods 161, all of which are spaced apart along the length of the side panels 16. Each set of limiting rods 161 abuts against the sidewall of the positioning rod 121 away from the base 11 to prevent the sample pool 12 from separating from the base 11.

[0045] Reference Figure 3 and Figure 4, each set of side panels 16 is provided with a waist-shaped groove 162 on the side wall facing the positioning rod 121, for the limiting rod 161 to be inserted into. An adjustment block 163 is slidably installed inside each set of waist-shaped grooves 162 of the side panel 16 along the height direction of the side panel 16, and the end of the limiting rod 161 away from the sample pool 12 is threadedly connected to the adjustment block 163. Several sets of adjustment bolts 164 are inserted into the side wall of the side panel 16 away from the sample pool 12, and the adjustment screws can slide along the height direction of the side panel 16. The adjustment screws and the adjustment blocks 163 are respectively provided in a one-to-one correspondence, and each set of adjustment screws is threadedly connected to the corresponding adjustment block 163 to fix the adjustment block 163 to the side panel 16.

[0046] Reference Figure 5 and Figure 6 A locking assembly 2 is installed between the base 11 and the sample cell 12. The locking assembly 2 is distributed on both sides of the base 11 in the width direction and is used to lock the base 11 and the sample cell 12. The locking assembly 2 includes a plug-in plate 21, a locking rod 22, and a driving member 23. The plug-in plate 21 is integrally formed at the end of the sample cell 12 and is located on the side wall of the sample cell 12 facing the base 11.

[0047] Reference Figure 5 and Figure 6 The top wall of the base 11 is provided with an insertion slot 111 along the vertical direction, into which the end of the plug-in plate 21 away from the sample cell 12 is slidably inserted. A locking slot 112 is provided inside the base 11, extending along the width of the base 11, and the locking slot 112 is connected to the interior of the insertion slot 111. The locking rod 22 is slidably mounted inside the locking slot 112, and a locking hole 211 is provided through the plug-in plate 21, into which the locking rod 22 is slidably pressed, thereby preventing the sample cell 12 from being separated from the base 11.

[0048] Reference Figure 5 and Figure 6 The driving member 23 is mounted on the base 11 and is used to drive the locking rod 22 toward or away from the plug board 21. The driving member 23 includes a guide rod 231, an anti-slip block 232, an elastic member 233, and a driving rod 234. The guide rod 231 slides through the interior of the base 11 and moves along the width direction of the base 11. One end of the guide rod 231 is adhesively connected to the end of the locking rod 22 away from the plug board 21, and the other end of the guide rod 231 is exposed outside the base 11. The anti-slip block 232 is threadedly connected to the end of the guide rod 231 away from the locking rod 22.

[0049] Reference Figure 5 and Figure 6In this embodiment, the elastic member 233 is located in a spring. The elastic member 233 is sleeved on the guide rod 231. One end of the elastic member 233 is adhesively connected to the side wall of the anti-slip block 232 facing the base 11, and the other end of the elastic member 233 is adhesively connected to the side wall of the base 11 facing the anti-slip block 232. The elastic member 233 is in a compressed state, so that the elastic force of the elastic member 233 itself drives the anti-slip block 232 to drive the guide rod 231 and the locking rod 22 away from the plug board 21. The drive rod 234 is fixedly connected to the translation frame 131 at the bottom of the cover 13, and the end of the drive rod 234 away from the cover 13 can abut against the side wall of the anti-slip block 232 facing away from the guide rod 231, thereby driving the guide rod 231 and the locking rod 22 to approach and penetrate the plug board 21.

[0050] Reference Figure 7 and Figure 8 Each set of side panels 16 is mounted with a splitter assembly 3 for separating the sealing gasket 15 from the sample cell 12. The splitter assembly 3 comprises a splitter plate 31, a sliding rod 32, and a drive cylinder 33. The sliding rod 32 slides through the side panels 16, and the splitter plate 31 is fixedly connected to the sidewalls of all the sliding rods 32 facing the sample cell 12. Several groups of vent holes 311 are defined through the sidewalls of the splitter plate 31, spaced along the length of the splitter plate 31. The drive cylinder 33 is fixedly connected to the side panels 16, with the output end of the drive cylinder 33 fixedly connected to the splitter plate 31. The output end of the drive cylinder 33 is retracted and extended to move the splitter plate 31 toward or away from the sample cell 12. A guide surface 312 is defined on the sidewall of the splitter plate 31 facing the sample cell 12, allowing it to be inserted between the sample cell 12 and the sealing gasket 15 to separate the sample cell 12 and the sealing gasket 15.

[0051] Reference Figure 7 and Figure 8 A plurality of vent holes 151 are formed on the sealing gasket 15 along the thickness direction. A sealing component 4 for sealing all the vent holes 151 is installed between the thermal cover 14 and the sealing gasket 15. A separation component 5 is installed on the thermal cover 14 to release the sealing component 4 from sealing all the vent holes 151.

[0052] Reference Figure 7 and Figure 8 The closing assembly 4 includes a closing plate 41, a closing head 42, a guide rod 43 and a pressing member 44; the closing plate 41 is located between the thermal cover 14 and the sealing gasket 15, and the guide rod 43 is fixedly connected to the side wall of the closing plate 41 facing the sealing gasket 15, and all the ends of the guide rods 43 away from the thermal cover 14 pass through the closing plate 41, so that the closing plate 41 slides along the length direction of the guide rods 43.

[0053] Reference Figure 7 and Figure 8The closing head 42 is integrally formed on the side wall of the closing plate 41 facing the sealing gasket 15. In this embodiment, the closing heads 42 correspond to the vents 151 one by one, and each group of closing heads 42 is located inside the corresponding vents 151 to block the vents 151. In this embodiment, the pressing member 44 is a spring. The pressing member 44 is located between the closing plate 41 and the thermal cover 14. One end of the pressing member 44 is adhesively connected to the closing plate 41, and the other end of the pressing member 44 is adhesively connected to the thermal cover 14. The pressing member 44 is in a compressed state, so that the elastic force of the pressing member 44 drives the closing plate 41 to drive the closing head 42 to stably seal the vents 151 on the sealing gasket 15.

[0054] Reference Figure 7 and Figure 8 The separation assembly 5 includes an extension rod 51 and a separation rod 52. The extension rod 51 is fixedly connected to the side wall of each set of sliding rods 32. The separation rod 52 is bent and formed at the end of each set of extension rods 51 away from the sliding rods 32, and the end of the separation rod 52 away from the extension rod 51 faces the thermal cover 14. A passage 141 is formed through the side wall of the thermal cover 14 for the separation rod 52 to pass through. The end of the separation rod 52 facing the thermal cover 14 is formed with a lifting slope 521 to facilitate lifting the sealing plate 41 and the sealing head 42 away from the sealing gasket 15.

[0055] The implementation principle of an automated PCR anti-splash device in the embodiment of the present application is as follows:

[0056] When the machine cover 13 drives the side plate 16 and the hot cover 14 to move above the sample pool 12, the limiting rod 161 follows the side plate 16 to move to the positioning rod 121. When the hot cover 14 is opened, the limiting rod 161 is used to abut against the side wall of the positioning rod 121 away from the base 11 to limit the sample pool 12 from following the hot cover 14 in the lifting and lowering movement, and quickly separate the sample pool 12 from the sealing gasket 15, thereby reducing the phenomenon of sample splashing caused by the sample pool 12 following the movement of the hot cover 14 when the hot cover 14 is opened.

[0057] The above are all preferred embodiments of the present application, and are not intended to limit the scope of protection of the present application. Therefore, any equivalent changes made based on the structure, shape, and principle of the present application should be included in the scope of protection of the present application.

Claims

1. An automated PCR anti-splash device, comprising a body (1) and a base (11) disposed at one end of the body (1), wherein the base (11) is provided with a sample pool (12) for storing a sample, a cover (13) is slidably disposed on the body (1), a heat cover (14) is provided inside the cover (13) for lifting, and a sealing gasket (15) is provided inside the heat cover (14) for sealing the sample pool (12); characterized in that: Positioning rods (121) are provided at both ends of the sample pool (12) in the longitudinal direction, and side panels (16) are provided on both sides of the cover (13) in the width direction. A limiting rod (161) is provided on each of the side panels (16), and the limiting rods (161) are arranged at intervals along the longitudinal direction of the side panels (16), and each of the limiting rods (161) abuts against the side wall of the positioning rod (121) away from the base (11); Each side plate (16) is provided with a waist-shaped groove (162) on the side wall facing the positioning rod (121) for the limiting rod (161) to be inserted into. An adjustment block (163) is provided on the side plate (16) so as to slide in the vertical direction inside the waist-shaped groove (162). The adjustment block (163) is connected to the limiting rod (161). An adjustment bolt (164) for adjusting the position of the adjustment block (163) is passed through the side plate (16). A locking assembly (2) is provided between the base (11) and the sample pool (12) for locking the base (11) and the sample pool (12); The locking assembly (2) includes a plug-in plate (21), a locking rod (22) and a driving member (23); the plug-in plate (21) is arranged on the side wall of the sample pool (12) facing the base (11); a plug-in slot (111) for the plug-in plate (21) to be inserted into the top wall of the base (11); a locking slot (112) communicating with the plug-in slot (111) is arranged inside the base (11); the locking rod (22) is slidably arranged inside the locking slot (112); the driving member (23) is arranged on the base (11) for driving the locking rod (22) to approach or move away from the plug-in plate (21); and a locking hole (211) for the locking rod (22) to pass through is provided on the plug-in plate (21); Each of the side plates (16) is provided with a splitting assembly (3) for splitting the sealing gasket (15) and the sample pool (12); The splitting assembly (3) comprises a splitting plate (31), a sliding rod (32) and a driving cylinder (33); the splitting plate (31) is slidably arranged between the side plate (16) and the sample pool (12); a plurality of exhaust holes (311) are provided through the side wall of the splitting plate (31), and a guide surface (312) is provided on the side wall of the splitting plate (31) facing the sample pool (12) for facilitating insertion between the sample pool (12) and the sealing gasket (15); the sliding rod (32) is arranged on the side wall of the splitting plate (31) away from the sample pool (12), and the end of the sliding rod (32) away from the splitting plate (31) passes through the side plate (16); the driving cylinder (33) is arranged on the side plate (16) for driving the splitting plate (31) to approach or move away from the sample pool (12); The sealing gasket (15) is provided with a plurality of groups of vent holes (151) extending therethrough. A sealing component (4) for sealing all the vent holes (151) is provided between the thermal cover (14) and the sealing gasket (15). A separating component (5) is provided on the thermal cover (14) for releasing the sealing of the vent holes (151) by the sealing component (4).

2. The automated PCR anti-splash device according to claim 1, characterized in that: The driving member (23) comprises a guide rod (231), an anti-slip block (232), an elastic member (233) and a driving rod (234); the guide rod (231) is arranged inside the base (11), one end of the guide rod (231) is connected to the end of the locking rod (22) away from the plug-in board (21), and the other end of the guide rod (231) is exposed on the base (11); the anti-slip block (232) is arranged at the end of the guide rod (231) away from the locking rod (22), and the elastic member (233) is arranged between the anti-slip block (232) and the base (11) to drive the locking rod (22) away from the plug-in board (21) through its own elastic force; the driving rod (234) is arranged on the machine cover (13), and the driving rod (234) can be abutted against the side wall of the anti-slip block (232) away from the guide plate.

3. The automated PCR anti-splash device according to claim 1, characterized in that: The sealing assembly (4) comprises a sealing plate (41), a sealing head (42), a guide rod (43) and a pressing member (44); the sealing plate (41) is slidably arranged between the thermal cover (14) and the sealing gasket (15); the sealing head (42) is arranged on the side wall of the sealing plate (41) facing the sealing gasket (15); the sealing head (42) and the vent holes (151) are respectively arranged in a one-to-one correspondence, and each of the sealing heads (42) is used to block the corresponding vent hole (151); the guide rod (43) is arranged on the side wall of the thermal cover (14) facing the sealing gasket (15), and the guide rod (43) passes through the sealing plate (41); the pressing member (44) is arranged between the sealing plate (41) and the thermal cover (14) to drive the sealing plate (41) to fit tightly against the sealing gasket (15) through its own elastic force.

4. The automated PCR anti-splash device according to claim 3, characterized in that: The separation assembly (5) comprises an extension rod (51) and a separation rod (52); the extension rod (51) is arranged on the sliding rod (32); the separation rod (52) is arranged on the side wall of the extension rod (51) facing the closing plate (41); a passage (141) for the separation rod (52) to pass through is provided through the side wall of the thermal cover (14); and a lifting inclined surface (521) is provided at the end of the separation rod (52) facing the closing plate (41) for lifting the closing plate (41) away from the sealing gasket (15).

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