Microorganism separation culture device for high-temperature white spirit yeast

Abstract

The invention discloses a microbe separation culture device for high-temperature liquor yeast, belongs to the technical field of microbe separation culture, and aims to solve the problems that an existing plate streaking method is operated manually, plate streaking needs to be carried out for multiple times, work is boring, and the error rate is high. The driving mechanism drives the sliding seat to move in the shell, the sliding seat drives the inoculating loop to be in a linear motion state in the front half moving process of the sliding seat, the inoculating loop reciprocates below the sliding seat in the rear half moving process of the sliding seat, and a continuous broken line motion track is achieved in cooperation with linear motion of the sliding seat; then, the upper part is driven to rotate during revolution, after position adjustment, residual microorganisms on the upper part are disinfected, then secondary scribing is performed, the tail part of the first scribing area is passed during secondary scribing, a small amount of microorganisms are carried, then the tail part of the second scribing area is carried during third scribing, and the operation is cyclically performed; therefore, the working efficiency is high, and the error rate is low.

Description

Technical Field

[0001] The present invention relates to the technical field of microbial separation and cultivation, in particular to a device for separating and cultivating microorganisms of high-temperature liquor Daqu. Background Art

[0002] The high-temperature liquor Daqu microbial separation and culture device is a device used to separate and culture microorganisms in high-temperature liquor Daqu. High-temperature liquor Daqu is an important fermentation agent necessary for the production of traditional Chinese liquor, which contains a variety of microorganisms such as yeast and bacteria. Separation and culture require that each microbial cell be independently separated and formed into a separate colony, followed by single colony selection and further culture and purification.

[0003] For the isolation and cultivation of high-temperature liquor Daqu microorganisms, the plate streaking method is a commonly used microbial isolation and cultivation method. However, during the actual cultivation operation, multiple plate streaking methods are required to increase the chance of biological cultivation. The existing plate streaking methods are all manual operations, which are tedious and have a high error rate. Therefore, a high-temperature liquor Daqu microbial isolation and cultivation device is proposed to facilitate the replacement of manual plate streaking operations. Summary of the Invention

[0004] The purpose of the present invention is to provide a high-temperature liquor Daqu microbial separation and cultivation device, which solves the problem that the existing plate streaking method in the background art is all manual operation, requires multiple plate streaking, the work is tedious and the error rate is high.

[0005] To achieve the above-mentioned objectives, the present invention provides the following technical solutions: A high-temperature white wine Daqu microbial separation and culture device, comprising a shell and a marking assembly arranged inside the shell, the marking assembly comprising a driving mechanism arranged inside the shell, and also comprising a sliding seat slidably arranged inside the shell, the driving mechanism comprising a screw rod rotatably connected to the inside of the shell, the sliding seat being threadedly connected to the screw rod, the driving mechanism being used to drive the sliding seat to horizontally move inside the shell, an inoculation ring being movably installed below the sliding seat, the sliding seat being used to drive the inoculation ring to move, a fixed plate being also provided on the top of the sliding seat, a reciprocating motion mechanism being provided inside the fixed plate, the reciprocating motion mechanism being used to drive the inoculation ring to slide back and forth below the sliding seat, a spur gear being connected to the top of the fixed plate above the reciprocating motion mechanism, the marking assembly also comprising a spur rack fixedly arranged inside the shell, the spur rack corresponding to the spur gear, the length of the spur rack being half of the screw rod, and the spur rack being located above the rear half of the screw rod.

[0006] Furthermore, the shell is a shell with an opening at the bottom, and a first inlet and a second inlet are provided below the outer side surface of the shell, wherein the second inlet is a sample entry and exit area.

[0007] Furthermore, a sliding groove is provided inside the shell, the sliding seat and the fixed plate are fixedly connected, and the sliding seat and the fixed plate are slidingly connected inside the sliding groove. A first rotating groove is also provided inside the shell, and the screw is rotatably connected inside the first rotating groove.

[0008] Furthermore, the driving mechanism also includes a servo motor fixedly arranged inside the housing, the output end of the servo motor is fixedly connected to a worm, the head end of the lead screw is fixedly connected to a worm wheel, and the worm wheel is meshingly connected to the worm.

[0009] Furthermore, a slide groove is provided at the bottom of the sliding seat, a slider is slidably connected in the slide groove, and the inoculation loop is fixedly installed at the bottom of the slider.

[0010] Furthermore, a movable groove is provided inside the fixed plate, the reciprocating motion mechanism includes a sector gear rotatably connected in the middle of the movable groove, the reciprocating motion mechanism also includes a movable ring slidably connected inside the movable groove, and tooth grooves corresponding to the sector gears are provided on both sides of the interior of the movable ring, and connecting rods are fixedly connected on both sides of the tooth grooves, and the other end of the connecting rod is fixedly connected to the slider.

[0011] Furthermore, the spur gear is fixedly connected to the sector gear via a shaft.

[0012] Furthermore, a bottom plate is slidably connected to the lower interior of the shell, an electric telescopic rod is connected between the top inner wall of the shell and the top of the bottom plate, a reduction motor is installed inside the bottom plate, and the output end of the reduction motor is fixedly connected to the first rotating seat.

[0013] Furthermore, a second rotating groove is provided on one side of the top of the first rotating seat, the second rotating groove is internally rotatably connected to the second rotating seat, a gear ring is provided on the outer side of the second rotating seat, grooves are provided on both sides of the outer side of the first rotating seat, and the gear ring on the outer side of the second rotating seat is exposed from the groove.

[0014] Furthermore, an interlocking groove is provided on the top of the second rotating seat, in which the bottom of the dish is interlocked and installed, an arc-shaped rack is fixedly connected to the inner wall of the side of the shell, the arc-shaped rack corresponds to the gear ring, and the tooth position on the arc-shaped rack is one-sixth of the tooth position of the gear ring, an alcohol lamp is interlocked and installed on the other side of the top of the first rotating seat, a pipe is installed on the alcohol lamp, the other end of the pipe corresponds to the inoculation ring, and a groove is provided on the top of the shell.

[0015] Compared with the prior art, the present invention has the following beneficial effects:

[0016] The present invention provides a high-temperature liquor Daqu microorganism separation and cultivation device, which drives the sliding seat to move inside the shell through the rotation of the screw rod in the driving mechanism. During the first half of the movement of the sliding seat, the sliding seat drives the inoculation loop to move in a linear state. During the second half of the movement of the sliding seat, the spur gear engages the spur rack to rotate, thereby driving the reciprocating motion mechanism to cause the inoculation loop to reciprocate under the sliding seat. In conjunction with the linear motion of the sliding seat, a continuous broken line motion trajectory is achieved. Subsequently, by driving the rotation, the upper part rotates during the revolution. After the position is adjusted, the residual microorganisms are disinfected and a second streaking is performed. During the second streaking, the tail of the first streaking area is passed through, carrying a small amount of microorganisms. Subsequently, the tail of the second streaking area is carried for the third streaking. The operation is cyclical until five streakings are completed, with high work efficiency and low error rate. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a schematic diagram of the overall structure of the present invention;

[0018] Figure 2 This is a diagram showing the overall structure of the present invention;

[0019] Figure 3 This is a cross-sectional view of the housing structure of the present invention;

[0020] Figure 4 It is a schematic structural diagram of the scribing assembly of the present invention;

[0021] Figure 5 The scribing assembly structure of the present invention explodes Figure 1 ;

[0022] Figure 6 The scribing assembly structure of the present invention explodes Figure 2 ;

[0023] Figure 7 An exploded view of the drive mechanism, sliding seat, and inoculating loop structure of the present invention;

[0024] Figure 8 Schematic diagram of the inoculating ring, reciprocating motion mechanism, spur gear and spur rack structure of the present invention;

[0025] Figure 9 This is an exploded view of the base plate, electric telescopic rod and first rotating seat structure of the present invention;

[0026] Figure 10 It is a drawing of the marking process of the present invention.

[0027] In the figure: 1. outer shell; 11. first insertion port; 12. second insertion port; 13. groove; 14. sliding groove; 15. first rotating groove; 2. marking assembly; 21. driving mechanism; 211. servo motor; 212. worm; 213. worm gear; 214. lead screw; 22. sliding seat; 221. sliding groove; 222. slider; 23. inoculating ring; 24. fixed plate; 241. movable groove; 25. reciprocating motion mechanism; 251. sector gear; 252. movable ring; 253. tooth groove; 254. connecting rod; 26. spur gear; 27. spur rack; 3. bottom plate; 31. reduction motor; 4. electric telescopic rod; 5. first rotating seat; 51. second rotating groove; 52. second rotating seat; 521. gear ring; 522. fitting groove; 53. arc rack; 54. dish bottom; 55. alcohol lamp; 551. pipeline. DETAILED DESCRIPTION

[0028] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0029] In order to solve the technical problems that the existing flat plate marking method is manual operation, multiple flat plate markings are required, the work is tedious and the error rate is high, such as Figures 1-10 As shown, the following preferred technical solutions are provided:

[0030] A high-temperature liquor Daqu microbial separation and culture device comprises a shell 1 and a marking assembly 2 arranged inside the shell 1, the marking assembly 2 comprises a driving mechanism 21 arranged inside the shell 1, and also comprises a sliding seat 22 slidably arranged inside the shell 1, the driving mechanism 21 comprises a screw rod 214 rotatably connected to the inside of the shell 1, the sliding seat 22 is threadedly connected to the screw rod 214, the driving mechanism 21 is used to drive the sliding seat 22 to move horizontally inside the shell 1, an inoculation ring 23 is movably installed below the sliding seat 22, the sliding seat 22 is used to drive the inoculation ring 23 to move, a fixed plate 24 is further provided on the top of the sliding seat 22, a reciprocating motion mechanism 25 is provided inside the fixed plate 24, the reciprocating motion mechanism 25 is used to drive the inoculation ring 23 to slide back and forth below the sliding seat 22, and a spur gear 26 is connected to the top of the fixed plate 24 above the reciprocating motion mechanism 25. 2 also includes a spur rack 27 fixedly arranged inside the housing 1. The spur rack 27 corresponds to the spur gear 26. The length of the spur rack 27 is half of the screw rod 214. The spur rack 27 is located above the rear half of the screw rod 214. The screw rod 214 in the driving mechanism 21 rotates to drive the sliding seat 22 to move inside the housing 1. During the first half of the movement of the sliding seat 22, the spur gear 26 does not contact the spur rack 27. The reciprocating mechanism 25 is in a stationary state. The inoculating ring 23 is fixed below the sliding seat 22. At this time, the sliding seat 22 drives the inoculating ring 23 to move linearly. During the second half of the movement of the sliding seat 22, the spur gear 26 engages the spur rack 27 to rotate, thereby driving the reciprocating mechanism 25 to cause the inoculating ring 23 to reciprocate below the sliding seat 22. Combined with the linear motion of the sliding seat 22, a continuous broken line motion trajectory is achieved, and finally a shape is formed as shown in FIG. Figure 10 A broken line trajectory among any of A, B, C, D and E shown.

[0031] The shell 1 is a shell with an opening at the bottom. A first inlet 11 and a second inlet 12 are provided at the lower outer side of the shell 1. The second inlet 12 is a sample entry and exit area. The sample is inserted from the second inlet 12 so that the inoculation loop 23 dips the sample.

[0032] A sliding groove 14 is provided inside the shell 1, the sliding seat 22 and the fixed plate 24 are fixedly connected, and the sliding seat 22 and the fixed plate 24 are slidingly connected inside the sliding groove 14. A first rotating groove 15 is also provided inside the shell 1, and the screw rod 214 is rotatably connected inside the first rotating groove 15.

[0033] The driving mechanism 21 also includes a servo motor 211 fixedly arranged inside the housing 1. The output end of the servo motor 211 is fixedly connected to a worm 212, and the head end of the screw rod 214 is fixedly connected to a worm wheel 213. The worm wheel 213 is meshed with the worm 212. By starting the servo motor 211 to drive the worm 212 to rotate, the threaded teeth on the outside of the worm 212 engage with the teeth on the outside of the worm wheel 213, thereby driving the screw rod 214 to rotate.

[0034] A slide groove 221 is provided at the bottom of the sliding seat 22 , a slider 222 is slidably connected in the slide groove 221 , and the inoculating loop 23 is fixedly installed at the bottom of the slider 222 .

[0035] A movable groove 241 is provided inside the fixed plate 24, and the reciprocating motion mechanism 25 includes a sector gear 251 rotatably connected in the middle of the movable groove 241. The reciprocating motion mechanism 25 also includes a movable ring 252 slidably connected inside the movable groove 241. Tooth grooves 253 corresponding to the sector gear 251 are provided on both sides of the interior of the movable ring 252. Connecting rods 254 are fixedly connected on both sides of the tooth grooves 253, and the other end of the connecting rod 254 is fixedly connected to the slider 222.

[0036] The spur gear 26 is fixedly connected to the sector gear 251 through an axis. When the spur gear 26 is engaged with the spur rack 27, the spur gear 26 rotates, thereby driving the sector gear 251 to rotate. The teeth on the outside of the sector gear 251 engage back and forth with the tooth grooves 253 on both sides of the inside of the movable ring 252, thereby driving the movable ring 252 to reciprocate, and finally driving the inoculation ring 23 to move back and forth under the action of the connecting rod 254 and the slider 222.

[0037] A base plate 3 is slidably connected to the lower part of the interior of the shell 1, and an electric telescopic rod 4 is connected between the top inner wall of the shell 1 and the top of the base plate 3. The upper and lower positions of the base plate 3 inside the shell 1 can be changed by extending and retracting the electric telescopic rod 4. A reduction motor 31 is installed inside the base plate 3, and the output end of the reduction motor 31 is fixedly connected to a first rotating seat 5, and the cross-section of the first rotating seat 5 is runway-shaped.

[0038] A second rotating groove 51 is provided on one side of the top of the first rotating seat 5, and the second rotating seat 52 is rotatably connected inside the second rotating groove 51. A gear ring 521 is provided on the outside of the second rotating seat 52. Grooves are provided on both sides of the outside of the first rotating seat 5, and the gear ring 521 on the outside of the second rotating seat 52 is exposed from the groove.

[0039] The top of the second rotating seat 52 is provided with an interlocking groove 522, and the dish bottom 54 is interlocked and installed in the interlocking groove 522. The inner wall of the side of the outer shell 1 is fixedly connected with an arc-shaped rack 53, which corresponds to the gear ring 521, and the tooth position on the arc-shaped rack 53 is one-sixth of the tooth position of the gear ring 521. An alcohol lamp 55 is interlocked and installed on the other side of the top of the first rotating seat 5. A pipe 551 is installed on the alcohol lamp 55, and a cotton core is installed in the pipe 551. One end of the cotton core is located inside the alcohol lamp 55, and the other end of the cotton core is located outside the pipe 551. The other end of the pipe 551 corresponds to the inoculation ring 23. When the first rotating seat 5 rotates, the other end of the pipe 551 is located directly below the inoculation ring 23, so that the inoculation ring 23 can be burned. A groove 13 is provided on the top of the outer shell 1, and the alcohol lamp 55 can be ignited or extinguished from the groove 13.

[0040] Specifically, first, the electric telescopic rod 4 is extended, the bottom plate 3 moves downward, and the dish bottom 54 is inserted into the first insertion port 11 and placed in the position of the insertion groove 522. Then, the electric telescopic rod 4 is retracted, and then the first rotating seat 5 is driven to rotate by the reduction motor 31, and the pipe 551 is ignited at the groove 13. Under the control of the reduction motor 31, the flame emitted from the other end of the pipe 551 burns and disinfects the inoculating loop 23. Since the first rotating seat 5 is in the shape of a runway, the dish bottom 54 and the alcohol lamp 55 are not in contact with the inoculating loop 2 under the control of the reduction motor 31. 3, the test tube containing the sample can be inserted from the second insertion port 12, so that the inoculating loop 23 is dipped with the sample. Then, under the control of the reduction motor 31, the bottom 54 of the dish and the inoculating loop 23 are aligned up and down. At this time, the electric telescopic rod 4 is controlled to be further retracted until the inoculating loop 23 contacts the inner surface of the bottom 54 of the dish. Then, the scribing assembly 2 is started. Under the control of the driving mechanism 21, the sliding seat 22, the fixed plate 24, the reciprocating motion mechanism 25, the spur gear 26 and the spur rack 27, the inoculating loop 23 can be completed inside the bottom 54 of the dish. Figure 10 The marking operation shown in the area A is then performed by extending the electric telescopic rod 4. The inoculating ring 23 is no longer in contact with the bottom 54 of the dish. The first rotating seat 5 is controlled by the reduction motor 31 to rotate counterclockwise. Under the action of the meshing of the arc-shaped rack 53, the engaging groove 522 rotates one-sixth of a turn clockwise. Then, driven by the reduction motor 31, the alcohol lamp 55 moves below the inoculating ring 23 to burn the inoculating ring 23 and kill its residual microorganisms. Then, when the bottom 54 of the dish is aligned with the inoculating ring 23 again, the marking assembly 2 is reset. Under the control of the electric telescopic rod 4, the inoculating ring 23 is in contact with the bottom 54 of the dish again. The marking assembly 2 can achieve the following operation. Figure 10During the marking operation for the middle B area, the second marking passes through the tail of the first marking area, carrying a small amount of microorganisms. Then the third marking carries the tail of the second marking area, and the operation is repeated until five markings are completed. Since the tooth position on the arc-shaped rack 53 is one-sixth of the tooth position on the gear ring 521, there is a certain interval between the fifth marking and the first marking area after the fifth marking is completed. At this point, the marking operations for the five areas A, B, C, D, and E are completed. Then, the electric telescopic rod 4 is controlled to extend, the dish bottom 54 is taken out, and constant temperature incubation is carried out.

[0041] It should be noted that, in this document, relational terms such as first and second, etc., are used only to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship or order between these entities or operations. Moreover, the terms "comprises," "comprising," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that includes a list of elements includes not only those elements but also other elements not explicitly listed, or elements inherent to such process, method, article, or apparatus.

[0042] The above description is only a preferred specific embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any technician familiar with the technical field, within the technical scope disclosed by the present invention, who makes equivalent replacements or changes based on the technical solution and inventive concept of the present invention, should be covered by the scope of protection of the present invention.

Claims

1. A high-temperature liquor koji microbial separation and culture device, comprising a housing (1) and a scribing assembly (2) disposed inside the housing (1), characterized in that: The scribing assembly (2) includes a driving mechanism (21) arranged inside the housing (1), and also includes a sliding seat (22) slidably arranged inside the housing (1), the driving mechanism (21) includes a screw rod (214) rotatably connected to the inside of the housing (1), the sliding seat (22) is threadedly connected to the screw rod (214), the driving mechanism (21) is used to drive the sliding seat (22) to move horizontally inside the housing (1), an inoculation ring (23) is movably installed below the sliding seat (22), the sliding seat (22) is used to drive the inoculation ring (23) to move, and a fixed Plate (24), a reciprocating mechanism (25) is provided inside the fixed plate (24), the reciprocating mechanism (25) is used to drive the inoculation ring (23) to slide back and forth below the sliding seat (22), and a spur gear (26) is connected to the top of the fixed plate (24) above the reciprocating mechanism (25), and the marking assembly (2) also includes a spur rack (27) fixedly provided inside the housing (1), the spur rack (27) corresponds to the spur gear (26), the length of the spur rack (27) is half of the screw rod (214), and the spur rack (27) is located above the rear half of the screw rod (214).

2. The high-temperature liquor Daqu microbial separation and culture device according to claim 1, characterized in that: The housing (1) is a shell with an opening at the bottom, and a first inlet (11) and a second inlet (12) are provided below the outer side surface of the housing (1), wherein the second inlet (12) is a sample entry and exit area.

3. The high-temperature liquor Daqu microbial separation and culture device according to claim 1, characterized in that: A sliding groove (14) is provided inside the housing (1), the sliding seat (22) and the fixed plate (24) are fixedly connected, and the sliding seat (22) and the fixed plate (24) are slidably connected inside the sliding groove (14), and a first rotating groove (15) is also provided inside the housing (1), and the screw rod (214) is rotatably connected inside the first rotating groove (15).

4. The high-temperature liquor Daqu microbial separation and cultivation device according to claim 1, characterized in that: The driving mechanism (21) further comprises a servo motor (211) fixedly arranged inside the housing (1); the output end of the servo motor (211) is fixedly connected to a worm (212); the head end of the lead screw (214) is fixedly connected to a worm wheel (213); and the worm wheel (213) is meshedly connected to the worm wheel (212).

5. The high-temperature liquor Daqu microbial separation and culture device according to claim 1, characterized in that: A slide groove (221) is provided at the bottom of the sliding seat (22), a slider (222) is slidably connected in the slide groove (221), and the inoculation ring (23) is fixedly mounted on the bottom of the slider (222).

6. The high-temperature liquor Daqu microbial separation and culture device according to claim 1, characterized in that: A movable groove (241) is provided inside the fixed plate (24), and the reciprocating motion mechanism (25) includes a sector gear (251) rotatably connected in the middle of the movable groove (241). The reciprocating motion mechanism (25) also includes a movable ring (252) slidably connected inside the movable groove (241), and tooth grooves (253) corresponding to the sector gear (251) are provided on both sides of the movable ring (252). Connecting rods (254) are fixedly connected to both sides of the tooth grooves (253), and the other end of the connecting rod (254) is fixedly connected to the slider (222).

7. The high-temperature liquor Daqu microbial separation and culture device according to claim 6, characterized in that: The spur gear (26) is fixedly connected to the sector gear (251) via a shaft.

8. The high-temperature liquor Daqu microbial separation and culture device according to claim 1, characterized in that: A bottom plate (3) is slidably connected to the interior lower portion of the housing (1); an electric telescopic rod (4) is connected between the top inner wall of the housing (1) and the top of the bottom plate (3); a reduction motor (31) is installed inside the bottom plate (3); and an output end of the reduction motor (31) is fixedly connected to a first rotating seat (5).

9. The high-temperature liquor Daqu microbial separation and culture device according to claim 8, characterized in that: A second rotating groove (51) is provided on one side of the top of the first rotating seat (5), the second rotating groove (51) is rotatably connected to the inside of the second rotating seat (52), a toothed ring (521) is provided on the outside of the second rotating seat (52), and grooves are provided on both sides of the outside of the first rotating seat (5), and the toothed ring (521) on the outside of the second rotating seat (52) is exposed from the groove.

10. The high-temperature liquor Daqu microbial separation and culture device according to claim 9, characterized in that: The top of the second rotating seat (52) is provided with an engaging groove (522), and a dish bottom (54) is engaged and installed in the engaging groove (522). The inner wall of the side of the shell (1) is fixedly connected with an arc-shaped rack (53), and the arc-shaped rack (53) corresponds to the gear ring (521), and the tooth position on the arc-shaped rack (53) is one-sixth of the tooth position of the gear ring (521). An alcohol lamp (55) is engaged and installed on the other side of the top of the first rotating seat (5), and a pipe (551) is installed on the alcohol lamp (55), and the other end of the pipe (551) corresponds to the inoculation ring (23). The top of the shell (1) is provided with a groove (13).

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