DNA test tube liquid concentration adjusting equipment and DNA test tube liquid adjusting system

Abstract

The invention provides DNA test tube liquid concentration adjusting equipment and a DNA test tube liquid adjusting system. The equipment comprises a DNA test tube liquid shaking device, a DNA tube liquid ultramicro test device and a DNA tube liquid concentration adjusting device, the DNA tube liquid concentration adjusting device comprises a DNA tube liquid adjusting mechanism and a DNA tube liquid diluting cup; the DNA tube liquid adjusting mechanism comprises a tube liquid adjusting base, a tube liquid adjusting mechanical arm and a tube liquid extracting needle; the tube liquid extraction needle is used for extracting the diluent in the DNA tube liquid dilution cup to the DNA test tubes when the concentration of the liquid in the DNA test tubes is greater than a preset concentration, so that the concentrations of the liquids in the DNA test tubes are equal. The tube liquid adjusting mechanical arm drives the tube liquid extracting needle to the DNA tube liquid diluting cup, diluent in the DNA tube liquid diluting cup is extracted into the DNA test tube, and then the testing and diluting operation is repeated till the concentration of liquid in the DNA test tube reaches the preset concentration.

Description

Technical Field

[0001] The present disclosure relates to the technical field of DNA liquid testing, and in particular to a DNA test tube liquid concentration adjustment device and a DNA test tube liquid adjustment system. Background Art

[0002] At present, plasmid DNA, as a conventional gene carrier in the field of genetic engineering technology, is widely used in the operation of constructing cloned cells. The extraction and purification of plasmid DNA is one of the most basic steps in molecular biology. Alkaline lysis is a classic method for extracting plasmids from cells, and the vast majority of commercially available plasmid extraction kits adopt alkaline lysis. Plasmid mini-extraction is a commonly used method for extracting experimental plasmid DNA. Many existing plasmid mini-extraction kits use centrifugation to remove the precipitate. Compared with the phenol extraction method, the reagents used in this method are less harmful to the human body and the operation is relatively simple. However, during the alkaline lysis experiment, a white flocculent precipitate will be produced, and it is generally necessary to remove the precipitate after centrifugation and separate the supernatant to obtain the plasmid DNA.

[0003] The aforementioned extraction method is only suitable for small-dose laboratory use. On the production line, the precipitate mixture must first be transferred to a centrifuge tube, centrifuged at high speed to concentrate the precipitate at the bottom of the tube, and then the supernatant must be carefully pipetted or decanted for production. However, the concentration of the sample DNA liquid in the centrifuge tube is often higher than the standard concentration, that is, higher than the required qualified concentration. This can easily lead to inconsistent DNA concentration within the centrifuge tube, significantly affecting the results of subsequent uses of the same batch of DNA liquid, and making the reliability of experimental results uncertain. Summary of the Invention

[0004] The purpose of the present invention is to overcome the deficiencies in the prior art and provide a DNA test tube liquid concentration adjustment device and a DNA test tube liquid adjustment system that effectively improve the consistency of DNA liquid concentration.

[0005] The purpose of this disclosure is achieved through the following technical solutions: A DNA test tube liquid concentration adjustment device comprises: a DNA test tube liquid shaking device, a DNA tube liquid ultra-trace testing device, and a DNA tube liquid concentration adjustment device; the DNA test tube liquid shaking device is used to shake the liquid in multiple DNA test tubes; the DNA tube liquid ultra-trace testing device is used to detect the concentration of the liquid in the DNA test tube; the DNA tube liquid concentration adjustment device comprises a DNA tube liquid adjustment mechanism and a DNA tube liquid dilution cup, the DNA tube liquid dilution cup is used to hold the dilution liquid, and the DNA tube liquid adjustment mechanism comprises a tube liquid adjustment base, a tube liquid adjustment mechanical arm, and a tube liquid extraction needle. The tube liquid adjustment robotic arm is arranged on the tube liquid adjustment base, and the tube liquid extraction needle is arranged on the tube liquid adjustment robotic arm. The tube liquid extraction needle is used to extract the liquid in the DNA test tube to the test position of the DNA tube liquid ultra-trace testing device to test the concentration of the liquid in the DNA test tube; the liquid extraction control end of the tube liquid extraction needle is connected to the tube liquid concentration adjustment end of the DNA tube liquid ultra-trace testing device. The tube liquid extraction needle is also used to extract the dilution liquid in the DNA tube liquid dilution cup to the DNA test tube when the concentration of the liquid in the DNA test tube is greater than the preset concentration, so as to make the concentration of the liquid in each DNA test tube equal.

[0006] In one embodiment, the DNA tube liquid adjustment mechanism further includes a liquid collection slide rail, which is installed on the tube liquid adjustment robot arm, and the tube liquid extraction needle is slidably connected to the liquid collection slide rail.

[0007] In one embodiment, the DNA tube liquid adjustment mechanism further includes a sampling slider, the sampling slider is slidably disposed on the liquid collection slide rail, and the tube liquid extraction needle is fixed on the sampling slider.

[0008] In one embodiment, the DNA tube liquid adjustment mechanism further includes a liquid level probe, which is disposed on the tube liquid adjustment robotic arm and is used to detect the liquid level of the liquid in the DNA test tube.

[0009] In one embodiment, the DNA tube liquid adjustment mechanism further includes an ultrasonic liquid level detector, which is installed on the tube liquid adjustment robotic arm and is used to recheck the liquid level of the liquid in the DNA test tube.

[0010] In one embodiment, the DNA tube-liquid calibration mechanism further includes a test tube grabbing cylinder, which is fixedly connected to the tube-liquid calibration robotic arm and is used to grab the DNA test tube.

[0011] In one embodiment, the DNA test tube liquid shaking device includes a DNA liquid oscillation component and a DNA test tube locking component. The DNA liquid oscillation component includes an oscillating polarization motor and a polarization plate. The rotating shaft of the oscillating polarization motor is connected to the polarization plate to cause the polarization plate to rotate eccentrically. The DNA test tube locking component includes a DNA test tube locking seat and a DNA test tube locking piece. The DNA test tube locking seat is connected to a side of the polarization plate facing away from the oscillating polarization motor. The DNA test tube locking seat has at least one test tube mounting area. The test tube mounting area is used to accommodate multiple test tube assembly fixtures. The test tube assembly fixture is used to hold DNA liquid. The DNA test tube locking piece is arranged on the DNA test tube locking seat, and the DNA test tube locking piece is also used to connect with the test tube assembly fixture to install the test tube assembly fixture on the DNA test tube locking seat; wherein, the DNA liquid oscillation component also includes an oscillation positioning plate and a laser sensor, the oscillation positioning plate is mounted on the rotating shaft of the oscillation polarization motor, the oscillation positioning plate is provided with a positioning window, the laser sensor is arranged adjacent to the oscillation positioning plate, and the output end of the laser sensor is used to connect with the central control end of the oscillation polarization motor, so that when the oscillation polarization motor is started or reset, the sensing end of the laser sensor is arranged opposite to the positioning window.

[0012] In one embodiment, the DNA tube liquid ultra-trace testing device includes a DNA test tube placement seat and a DNA tube liquid concentration testing assembly, the DNA test tube placement seat is used to place DNA test tubes in batches; the DNA tube liquid concentration testing assembly includes a tube liquid concentration detection component and a lens liquid detection component, the tube liquid concentration detection component includes a liquid detection base, a liquid detection substrate, a liquid detection top plate and a detection rotating motor, the liquid detection substrate is arranged on the liquid detection base, the liquid detection top plate is rotatably connected to the liquid detection substrate, the detection rotating motor is connected to the liquid detection base, and the rotating shaft of the detection rotating motor is connected to the liquid detection top plate, so that the liquid detection top plate is flipped to fit the liquid detection substrate during the tube liquid test; the lens liquid detection component includes a lens liquid cleaning rack and a lens liquid cleaner, the lens liquid cleaning rack is arranged adjacent to the liquid detection base, the lens liquid cleaner is slidably arranged on the lens liquid cleaning rack, and the wiping end of the lens liquid cleaner is used to swing and wipe the liquid detection substrate.

[0013] In one embodiment, the lens liquid cleaner includes a liquid cleaning fixed plate, a tape feed wheel, a tape take-up wheel and a liquid cleaning extrusion head, the liquid cleaning fixed plate is arranged on the lens liquid cleaning rack, the tape feed wheel, the tape take-up wheel and the liquid cleaning extrusion head are all arranged on the liquid cleaning fixed plate, the tape feed wheel is used to unwind the liquid absorbing tape, the tape take-up wheel is used to rewind the liquid absorbing tape, the liquid cleaning extrusion head is located between the tape feed wheel and the tape take-up wheel, and the liquid cleaning extrusion head is used to squeeze the liquid absorbing tape onto the liquid detection substrate; the DNA tube liquid concentration test assembly also includes a liquid cleaning position adjusting part and a liquid cleaning swinging part, the liquid cleaning position adjusting part includes a liquid cleaning position adjusting motor, a liquid cleaning position adjusting driving plate and a liquid cleaning position adjusting moving rail, the liquid cleaning position adjusting The positioning motor is arranged on the lens clear liquid rack, and the clear liquid positioning drive plate is respectively connected with the telescopic shaft of the clear liquid positioning motor and the clear liquid fixed plate, and the clear liquid positioning movable rail is arranged on the clear liquid positioning drive plate, and the clear liquid positioning motor slides on the clear liquid positioning movable rail; the clear liquid swinging part includes a clear liquid swinging motor, a clear liquid swinging gear rod and a clear liquid swinging gear, the clear liquid swinging motor is fixed on the clear liquid fixed plate, the telescopic shaft of the clear liquid swinging motor is connected with the clear liquid swinging gear rod, the clear liquid swinging gear rod is meshed with the clear liquid swinging gear, the clear liquid swinging gear is rotatably arranged on the clear liquid fixed plate, and the central axis of the clear liquid swinging gear is connected to the clear liquid extrusion head.

[0014] A DNA test tube liquid calibration system includes the DNA test tube liquid concentration calibration device described in any of the above embodiments.

[0015] Compared with the prior art, the present disclosure has at least the following advantages: After the concentration test of the DNA test tube liquid, if the concentration of the DNA test tube liquid is too high, the tube liquid adjustment robot arm drives the tube liquid extraction needle to the DNA tube liquid dilution cup, extracts the diluted liquid in the DNA tube liquid dilution cup into the DNA test tube, and then repeats the above testing and dilution operations until the concentration of the liquid in the DNA test tube reaches the predetermined concentration, so that the concentration of the liquid in each DNA test tube is consistent. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] In order to more clearly illustrate the technical solutions of the embodiments of the present disclosure, the following briefly introduces the drawings required for use in the embodiments. It should be understood that the following drawings only illustrate certain embodiments of the present disclosure and therefore should not be regarded as limiting the scope. For ordinary technicians in this field, other relevant drawings can be obtained based on these drawings without creative work.

[0017] Figure 1 A schematic diagram of a device for adjusting the concentration of liquid in a DNA test tube according to an embodiment; Figure 2A schematic diagram of a DNA tube liquid concentration adjustment device in one embodiment; Figure 3 Schematic diagram of a DNA test tube liquid shaking device in one embodiment; Figure 4 for Figure 3 A schematic diagram of the DNA test tube liquid shaking device from another perspective; Figure 5 Schematic diagram of a DNA tube liquid ultra-micro test device in one embodiment; Figure 6 A schematic diagram of a DNA tube liquid concentration test assembly in one embodiment; Figure 7 for Figure 6 A schematic diagram of the DNA tube liquid concentration test assembly from another perspective is shown; Figure 8 for Figure 6 A schematic diagram of the DNA tube liquid concentration test assembly from another perspective is shown; Figure 9 Schematic diagram of the combination of a DNA test tube cover lifting assembly and a DNA test tube cover buckling assembly in one embodiment. DETAILED DESCRIPTION

[0018] To facilitate understanding of the present disclosure, a more comprehensive description of the present disclosure will be provided below with reference to the accompanying drawings. The accompanying drawings illustrate preferred embodiments of the present disclosure. However, the present disclosure can be implemented in many different forms and is not limited to the embodiments described herein. Rather, these embodiments are provided to provide a more thorough and comprehensive understanding of the disclosure.

[0019] It should be noted that when an element is referred to as being "fixed to" another element, it may be directly attached to the other element or there may be an intermediate element. When an element is referred to as being "connected to" another element, it may be directly connected to the other element or there may be an intermediate element. The terms "vertical," "horizontal," "left," "right," and similar expressions used herein are for illustrative purposes only and do not represent the only implementation methods.

[0020] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by those skilled in the art to which this disclosure pertains. The terms used herein in the specification of this disclosure are for the purpose of describing specific embodiments only and are not intended to limit this disclosure. The term "and / or" as used herein includes any and all combinations of one or more of the associated listed items.

[0021] The present disclosure relates to a DNA test tube liquid concentration adjustment device. In one embodiment, the DNA test tube liquid concentration adjustment device includes a DNA test tube liquid shaking device, a DNA tube liquid ultra-micro testing device, and a DNA tube liquid concentration adjustment device; the DNA test tube liquid shaking device is used to shake the liquid in multiple DNA test tubes; the DNA tube liquid ultra-micro testing device is used to detect the concentration of the liquid in the DNA test tube; the DNA tube liquid concentration adjustment device includes a DNA tube liquid adjustment mechanism and a DNA tube liquid dilution cup, the DNA tube liquid dilution cup is used to hold the dilution liquid, the DNA tube liquid adjustment mechanism includes a tube liquid adjustment base, a tube liquid adjustment mechanical arm, and a tube liquid The extraction needle, the tube liquid adjustment robot arm is arranged on the tube liquid adjustment base, and the tube liquid extraction needle is arranged on the tube liquid adjustment robot arm. The tube liquid extraction needle is used to extract the liquid in the DNA test tube to the test position of the DNA tube liquid ultra-micro testing device to test the concentration of the liquid in the DNA test tube; the liquid extraction control end of the tube liquid extraction needle is connected to the tube liquid concentration adjustment end of the DNA tube liquid ultra-micro testing device. The tube liquid extraction needle is also used to extract the dilution liquid in the DNA tube liquid dilution cup into the DNA test tube when the concentration of the liquid in the DNA test tube is greater than the preset concentration, so as to make the concentration of the liquid in each DNA test tube equal. After the concentration test of the DNA test tube liquid, if the concentration of the DNA test tube liquid is too high, the tube liquid adjustment robot arm drives the tube liquid extraction needle to the DNA tube liquid dilution cup, extracts the dilution liquid in the DNA tube liquid dilution cup into the DNA test tube, and then repeats the above test and dilution operation until the concentration of the liquid in the DNA test tube reaches the preset concentration, so that the concentration of the liquid in each DNA test tube remains consistent.

[0022] See also Figure 1 , which is a structural schematic diagram of a DNA test tube liquid concentration adjustment device according to an embodiment of the present disclosure.

[0023] The DNA test tube liquid concentration adjustment device 10 of one embodiment includes a DNA test tube liquid shaking device 10A, a DNA test tube liquid ultra-micro testing device 10B, and a DNA test tube liquid concentration adjustment device 10C. The DNA test tube liquid shaking device 10A is used to shake the liquid in multiple DNA test tubes. The DNA test tube liquid ultra-micro testing device 10B is used to detect the concentration of the liquid in the DNA test tube. Please refer to Figure 2The DNA liquid concentration adjustment device 10C includes a DNA liquid adjustment mechanism 100 and a DNA liquid dilution cup 200. The DNA liquid dilution cup 200 is used to hold the diluent. The DNA liquid adjustment mechanism 100 includes a liquid adjustment base 110, a liquid adjustment robot 120, and a liquid extraction needle 130. The liquid adjustment robot 120 is mounted on the liquid adjustment base 110. The liquid extraction needle 130 is mounted on the liquid adjustment robot 120. The liquid extraction needle 130 is used to extract the liquid in the DNA test tube to the testing position of the DNA liquid ultra-micro testing device 10B to test the concentration of the liquid in the DNA test tube. The liquid extraction control end of the tube liquid extraction needle 130 is connected to the tube liquid concentration adjustment end of the DNA tube liquid ultra-trace testing device 10B. The tube liquid extraction needle 130 is also used to extract the diluted liquid in the DNA tube liquid dilution cup 200 into the DNA test tube when the concentration of the liquid in the DNA test tube is greater than the preset concentration, so as to make the concentration of the liquid in each DNA test tube equal.

[0024] In this embodiment, after the concentration test of the DNA test tube liquid, if the concentration of the DNA test tube liquid is too high, the tube liquid adjustment robot 120 drives the tube liquid extraction needle 130 to the DNA tube liquid dilution cup 200 to extract the diluted liquid in the DNA tube liquid dilution cup 200 into the DNA test tube. Thereafter, the above-mentioned testing and dilution operations are repeated until the concentration of the liquid in the DNA test tube reaches the predetermined concentration, so that the concentration of the liquid in each DNA test tube remains consistent.

[0025] In another embodiment, the liquid extraction needle is a replaceable TIP needle, that is, the liquid extraction needle has multiple TIP needles connected in series. After each liquid extraction, the original TIP needle is automatically removed and a new TIP needle is used to extract the liquid to avoid cross contamination of the liquid.

[0026] In one embodiment, see Figure 2 The DNA tube liquid calibration mechanism 100 further includes a liquid collection slide 140, which is mounted on the tube liquid calibration robot arm 120. The tube liquid extraction needle 130 is slidably connected to the liquid collection slide 140. In this embodiment, the liquid collection slide 140 is located on the tube liquid calibration robot arm 120, and the tube liquid calibration robot arm 120 performs liquid collection operations through the tube liquid extraction needle 130. Specifically, the tube liquid calibration robot arm 120 is a robot arm with 4 or more degrees of freedom. The end of the tube liquid calibration robot arm 120 is connected to the liquid collection slide 140. The tube liquid extraction needle 130 slides along the direction of the liquid collection slide 140, which facilitates adjustment of the penetration depth of the tube liquid extraction needle 130 in the DNA test tube and the DNA tube liquid dilution cup 200, so that the DNA liquid dosage extracted by the tube liquid extraction needle 130 is more accurate.

[0027] Further, see Figure 2 The DNA tube liquid calibration mechanism 100 further includes a sampling slider 150, which is slidably disposed on the liquid collection rail 140. The liquid collection needle 130 is fixed to the sampling slider 150. In this embodiment, the sampling slider 150 is located between the liquid collection rail 140 and the liquid collection needle 130. The sampling slider 150 serves as a base for the liquid collection needle 130 to slide on the liquid collection rail 140. That is, the liquid collection needle 130 slides on the liquid collection rail 140 via the sampling slider 150. The sampling slider 150 acts as a sliding member for the liquid collection needle 130, preventing friction between the liquid collection needle 130 and the liquid collection rail 140, thereby reducing the chance of damage to the liquid collection needle 130 and ensuring stable and safe liquid collection by the liquid collection needle 130.

[0028] In one embodiment, see Figure 2 The DNA tube liquid calibration mechanism 100 further includes a liquid level probe 160, which is disposed on the tube liquid calibration robot arm 120. The liquid level probe 160 is used to detect the liquid level of the liquid in the DNA test tube. In this embodiment, the liquid level probe 160 is mounted on the tube liquid calibration robot arm 120. Specifically, the tube liquid calibration robot arm 120 has a liquid level detection area, and the liquid level probe 160 is disposed in the liquid level detection area. Moreover, the liquid level detection area is separated from the known area of the tube liquid extraction needle 130 to prevent the liquid level probe 160 from being too close to the tube liquid extraction needle 130, thereby preventing the liquid on the liquid level probe 160 from splashing onto the tube liquid extraction needle 130 and causing contamination of the extracting liquid, thereby ensuring the accuracy of the concentration test of the DNA liquid extracted by the tube liquid extraction needle 130.

[0029] In one embodiment, see Figure 2The DNA tube liquid calibration mechanism 100 further includes an ultrasonic liquid level detector 170, which is mounted on the tube liquid calibration robotic arm 120. The ultrasonic liquid level detector 170 is used to recheck the liquid level of the liquid in the DNA test tube. In this embodiment, the ultrasonic liquid level detector 170 is located on the tube liquid calibration robotic arm 120. Specifically, the ultrasonic liquid level detector 170 is disposed adjacent to the liquid level detection area, so that the ultrasonic liquid level detector 170 is close to the liquid level probe 160. The ultrasonic liquid level detector 170 determines the liquid level in the DNA test tube by ultrasonic positioning. When the liquid level probe 160 performs liquid level detection, the ultrasonic liquid level detector 170 simultaneously performs a secondary liquid level detection to calibrate the liquid level detected by the liquid level probe 160, thereby improving the detection accuracy of the liquid level in the DNA test tube.

[0030] In one embodiment, see Figure 2 The DNA tube and liquid calibration mechanism 100 further includes a test tube grabbing cylinder 180, which is fixedly connected to the tube and liquid calibration robot arm 120 and is used to grab DNA test tubes. In this embodiment, the test tube grabbing cylinder 180 is mounted on the tube and liquid calibration robot arm 120. As a grabbing component for DNA test tubes, the cylinder 180 facilitates loading, unloading, and transporting of DNA test tubes, thereby facilitating the transfer of DNA test tubes to corresponding workstations.

[0031] In one embodiment, see Figure 3 The DNA test tube liquid shaking device 10A includes a DNA liquid oscillating component 300 and a DNA test tube locking component 400. The DNA liquid oscillating component 300 includes an oscillating polarization motor 310 and a polarization plate 320. The rotating shaft of the oscillating polarization motor 310 is connected to the polarization plate 320 to make the polarization plate 320 rotate eccentrically; the DNA test tube locking component 400 includes a DNA test tube locking seat 410 and a DNA test tube locking member 420. The DNA test tube locking seat 410 The DNA test tube locking seat 410 is connected to a side of the polarization plate 320 facing away from the oscillating polarization motor 310. The DNA test tube locking seat 410 has at least one test tube installation area, which is used to accommodate a variety of test tube assembly fixtures, and the test tube assembly fixtures are used to hold DNA liquid; the DNA test tube locking piece 420 is set on the DNA test tube locking seat 410, and the DNA test tube locking piece 420 is also used to connect with the test tube assembly fixture to install the test tube assembly fixture on the DNA test tube locking seat 410.

[0032] In this embodiment, the DNA test tube locking seat 410 is used to place various types of test tube assembly fixtures, and the DNA test tube locking piece 420 is used to fix various types of test tube assembly fixtures on the DNA test tube locking seat 410 to improve the shaking of test tube types; the oscillating polarization motor 310 drives the polarization plate 320 to shake, and after the test tube assembly fixture is stabilized on the DNA test tube locking seat 410, the oscillating polarization motor 310 shakes the DNA liquid in the test tube on the test tube assembly fixture in batches and quickly, effectively reducing the production cost of shaking the test tube liquid.

[0033] In one embodiment, see Figure 3 The DNA test tube locking seat 410 is provided with a plurality of test tube locking grooves 402, which are used to secure portions of the test tube assembly fixture. In this embodiment, the test tube locking grooves 402 are located on the DNA test tube locking seat 410 and serve as fixed mounting grooves for the test tube assembly fixture. Specifically, the test tube locking grooves 402 are located within the test tube mounting area, and portions of the test tube assembly fixture are accommodated within the test tube locking grooves 402. The mounting position formed by the test tube locking grooves 402 facilitates embedding the test tube assembly fixture within the DNA test tube locking seat 410, thereby improving the installation stability of the test tube assembly fixture on the DNA test tube locking seat 410.

[0034] Furthermore, the multiple test tube locking grooves 402 are arranged parallel to each other. In this embodiment, the test tube locking grooves 402 fix the test tube assembly fixture to the DNA test tube locking base 410. The multiple test tube locking grooves 402 remain parallel, so that the multiple test tube assembly fixtures are synchronously and parallelly arranged, thereby increasing the number of test tube assembly fixtures on the DNA test tube locking base 410, facilitating batch shaking of test tubes.

[0035] In another embodiment, the test tube locking groove 402 is a U-shaped through groove, and the upper part of the test tube locking groove 402 has an opening, and the test tube assembly fixture has an I-shaped structure, so that the test tube assembly fixture is adapted to the test tube locking groove 402, making it convenient for the test tube assembly fixture to enter from the entrance of the test tube locking groove 402, so that the upper opening of the test tube locking groove 402 clamps the middle part of the I-shaped structure of the test tube assembly fixture.

[0036] In one embodiment, see Figure 3The DNA test tube lock 420 includes a locking outer plate 422 and a locking inner plate 424 that are interlocked. The locking outer plate 422 is connected to the DNA test tube locking seat 410, and the locking inner plate 424 is located between the locking outer plate 422 and the test tube assembly fixture. The locking outer plate 422 and the locking inner plate 424 span the plurality of test tube locking grooves 402. In this embodiment, the locking inner plate 424 abuts against the locking outer plate 422 and the test tube assembly fixture, respectively, and serves as a buffer plate for the fixed compression between the locking outer plate 422 and the test tube assembly fixture. The locking outer plate 422 and the locking inner plate 424 correspond to the upper openings of each test tube locking groove 402, so that the locking outer plate 422 and the locking inner plate 424 confine the test tube assembly fixture within the test tube locking groove 402 to improve the installation stability of the test tube assembly fixture, thereby improving the stability of the test tube assembly fixture during vibration.

[0037] In another embodiment, the locking outer plate 422 and the locking inner plate 424 are adjacent to the installation entrance of the test tube locking groove 402. In this embodiment, the locking outer plate 422 and the locking inner plate 424 correspond to the installation entrance of the test tube locking groove 402. Specifically, the locking outer plate 422 and the locking inner plate 424 are located above the test tube locking groove 402 and adjacent to the installation entrance of the test tube locking groove 402, so that the locking inner plate 424 abuts against the end of the test tube assembly fixture, thereby facilitating the confinement of the test tube assembly fixture within the test tube locking groove 402.

[0038] Further, see Figure 3 The DNA test tube lock 420 further includes a locking connecting rod 426, which is rotatably connected to the DNA test tube locking seat 410 and engages with the locking outer plate 422. In this embodiment, the locking connecting rod 426 is rotatably mounted on the DNA test tube locking seat 410. Specifically, one end of the locking connecting rod 426 is rotatably connected to the side wall of the DNA test tube locking seat 410, and the other end of the locking connecting rod 426 engages with the locking outer plate 422. The rotational connection of the locking connecting rod 426 to the locking outer plate 422 facilitates securing the locking outer plate 422 to the DNA test tube locking seat 410. Furthermore, the rotational connection of the locking connecting rod 426 facilitates disassembly and maintenance of the locking outer plate 422.

[0039] In another embodiment, see Figure 3The locking connecting rod 426 defines a locking snap groove 404, the notch of which faces the locking outer plate 422. The locking snap groove 404 is configured to receive a portion of the locking outer plate 422 so that the portion of the locking outer plate 422 is snapped into the locking snap groove 404. In this embodiment, the notch of the locking snap groove 404 faces downward, specifically, the notch of the locking snap groove 404 faces the polarizing plate 320, so that the locking connecting rod 426 can be snapped into the locking outer plate 422 by an inverted snap.

[0040] In one embodiment, see Figure 3 The DNA test tube lock 420 further includes a rotatably connected locking lever 428 and a locking buckle 421. The locking lever 428 is rotatably connected to the DNA test tube locking seat 410. The connection point between the locking lever 428 and the DNA test tube locking seat 410 is located between the rotational connection point between the locking buckle 421 and the locking lever 428 and the locking outer plate 422. The locking buckle 421 is sleeved onto the locking outer plate 422. In this embodiment, there is a rotation point between the locking lever 428 and the DNA test tube locking seat 410, and another rotation point between the locking lever 428 and the locking buckle 421. The locking buckle 421 is also sleeved onto the locking outer plate 422, that is, a portion of the locking outer plate 422 is located within the locking buckle 421. The locking buckle 421 is conveniently sleeved onto the locking outer plate 422 by rotating with the locking lever 428. When the locking lever 428 rotates relative to the DNA test tube locking seat 410 and the end of the locking lever 428 rotates toward and away from the locking outer plate 422, the locking buckle 421 is driven by the locking lever 428 to move toward and away from the locking outer plate 422, so that the locking buckle 421 stably squeezes the locking outer plate 422 onto the test tube assembly fixture, thereby making the installation of the test tube assembly fixture on the DNA test tube locking seat 410 more stable.

[0041] In another embodiment, the DNA test tube locking member is a bolt, and the test tube assembly fixture is an integral test kit having a plurality of liquid receiving grooves distributed in an array, so as to facilitate screwing the test tube assembly fixture onto the DNA test tube locking seat.

[0042] In one embodiment, see Figure 4The DNA liquid oscillation assembly 300 further includes an oscillation positioning disk 330 and a laser sensor 340. The oscillation positioning disk 330 is mounted on the rotating shaft of the oscillation polarization motor 310. The oscillation positioning disk 330 defines a positioning window 302. The laser sensor 340 is positioned adjacent to the oscillation positioning disk 330. The output end of the laser sensor 340 is connected to the central control end of the oscillation polarization motor 310, so that when the oscillation polarization motor 310 is started or reset, the sensing end of the laser sensor 340 is positioned opposite the positioning window 302. In this embodiment, the oscillation positioning disk 330 is mounted on the rotating shaft of the oscillation polarization motor 310 and deflects in accordance with the rotation of the rotating shaft of the oscillation polarization motor 310, such that the degree of deflection of the oscillation positioning disk 330 corresponds to the degree of shaking of the test tube assembly fixture. The sensing end of the laser sensor 340 cooperates with the positioning window 302 on the oscillating positioning disk 330. When the sensing end of the laser sensor 340 is aligned with the positioning window 302 on the oscillating positioning disk 330, the oscillating polarization motor 310 is in its initial or reset state, i.e., its initial shaking position. At this time, the light emitted by the sensing end of the laser sensor 340 passes through the positioning window 302, and no reflected light is received. However, when the oscillating polarization motor 310 is in the shaking state, the oscillating positioning disk 330 deflects, causing the light emitted by the sensing end of the laser sensor 340 to deviate from the positioning window 302. In other words, the light is reflected by the oscillating positioning disk 330, thereby receiving the reflected light. In this way, the laser sensor 340 determines and adjusts the operating state of the oscillating polarization motor 310 based on the received reflected light, so that the test tubes on the test tube assembly fixture remain stable after the shaking is completed.

[0043] In another embodiment, the laser sensor 340 is a through-beam sensor, that is, the sensing end of the laser sensor 340 includes a light receiving probe and a light emitting probe. The light receiving probe and the light emitting probe are arranged opposite to each other, and the light receiving probe and the light emitting probe both correspond to the positioning window 302. In the initial or reset state, the light emitted by the light emitting probe is received by the light receiving probe, and in the shaking state, the light emitted by the light emitting probe is blocked by the oscillating positioning plate 330, and the light cannot be received by the light receiving probe.

[0044] In one embodiment, see Figure 3The DNA test tube liquid shaking device 10A also includes a DNA test tube code collection component 430, which includes a test tube code collector 432 and a code collection movable rail 434. The code collection movable rail 434 is arranged adjacent to the DNA test tube locking seat 410, and the test tube code collector 432 is slidably arranged on the code collection movable rail 434. The scanning terminal of the test tube code collector 432 is facing the DNA test tube locking seat 410 to collect the code of each test tube assembly fixture. In this embodiment, the test tube code collector 432 serves as a collector for the codes of each test tube assembly fixture. The test tube code collector 432 moves along the direction of the code collection movable rail 434. The scanning terminal of the test tube code collector 432 is facing the test tube assembly fixture. When multiple test tube assembly fixtures are installed on the DNA test tube locking seat 410 in sequence, the test tube code collector 432 moves gradually on the code collection movable rail 434 to facilitate the entry of the codes of each test tube assembly fixture separately, so that the coding information of each test tube assembly fixture can be accurately collected.

[0045] In another embodiment, the code collecting movable rail 434 and the test tube locking groove 402 are perpendicular to each other. In this way, after each test tube assembly fixture is loaded and the test tube code collector 432 collects the code of the test tube assembly fixture, the test tube code collector 432 moves a predetermined distance along the code collecting movable rail 434 and in a direction away from the test tube assembly fixture to facilitate scanning of the next loaded test tube assembly fixture.

[0046] In one embodiment, see Figure 3 The DNA test tube liquid shaking device 10A further includes a test tube leveling detector 440, which is connected to the oscillating polarization motor 310. The detector 440's probe faces the test tube assembly fixtures to detect the height of each test tube assembly fixture. In this embodiment, the test tube leveling detector 440 serves as a height detector for multiple test tube assembly fixtures. By measuring the height of each test tube assembly fixture, it facilitates the installation and leveling verification of the multiple test tube assembly fixtures, ensuring a more stable installation of the multiple test tube assembly fixtures on the DNA test tube locking base 410, thereby facilitating batch and synchronous shaking of the test tube liquids.

[0047] In one embodiment, see Figure 5 The DNA tube liquid ultra-micro testing device 10B includes a DNA test tube placement seat 500 and a DNA tube liquid concentration testing component 600. The DNA test tube placement seat 500 is used to place DNA test tubes in batches; please refer to Figure 6The DNA tube liquid concentration test component 600 includes a tube liquid concentration detection component 610 and a lens clear liquid detection component 620. The tube liquid concentration detection component 610 includes a liquid detection base 612, a liquid detection substrate 614, a liquid detection top plate 616 and a detection rotation motor 618. The liquid detection substrate 614 is arranged on the liquid detection base 612, the liquid detection top plate 616 is rotatably connected to the liquid detection substrate 614, the detection rotation motor 618 is connected to the liquid detection base 612, and the detection The rotating shaft of the rotating motor 618 is connected to the liquid detection top plate 616, so that the liquid detection top plate 616 can be flipped over to fit with the liquid detection substrate 614 during the tube liquid test; the lens liquid cleaning component 620 for detecting the lens liquid cleaning includes a lens liquid cleaning rack 622 and a lens liquid cleaner 624, the lens liquid cleaning rack 622 is arranged adjacent to the liquid detection base 612, and the lens liquid cleaner 624 is slidably arranged on the lens liquid cleaning rack 622, and the wiping end of the lens liquid cleaner 624 is used to swing and wipe the liquid detection substrate 614.

[0048] In this embodiment, before the tube liquid test, the liquid taking component drops the tube liquid onto the liquid detection substrate 614, and the detection rotating motor 618 buckles the liquid detection top plate 616 on the liquid detection substrate 614 to form a test sample chamber. After the test is completed, the detection rotating motor 618 opens the liquid detection top plate 616. At this time, the lens liquid cleaner 624 moves to the liquid detection substrate 614 and wipes the residual tube liquid on the liquid detection substrate 614 by swinging, thereby effectively improving the efficiency of the tube liquid test.

[0049] In another embodiment, the tube liquid concentration detection component is an ultra-micro spectrophotometer.

[0050] In one embodiment, see Figure 6The tube liquid concentration detection member 610 further includes a rotating shaft extension rod 611 and a top sheet flipping rod 613, which are interconnected. The rotating shaft extension rod 611 is connected to the rotating shaft of the detection rotation motor 618. The top sheet flipping rod 613 is located at the end of the rotating shaft extension rod 611 away from the rotating shaft of the detection rotation motor 618. The top sheet flipping rod 613 is connected to a surface of the liquid detection top sheet 616 that faces away from the liquid detection substrate 614. In this embodiment, the rotating shaft extension rod 611 serves as a flip extension rod for the detection rotation motor 618 and rotates around the rotating shaft of the detection rotation motor 618. The top sheet flip rod 613 serves as a flipping component for the liquid detection substrate 614. The detection rotating motor 618 drives the top sheet flip rod 613 to rotate through the rotating shaft extension rod 611, so that the top sheet flip rod 613 drives the liquid detection top sheet 616 to rotate, so that the liquid detection top sheet 616 moves away from or close to the liquid detection substrate 614, which facilitates the opening and closing operation of the liquid detection top sheet 616 and the liquid detection substrate 614.

[0051] In another embodiment, the shaft extension rod 611 is respectively arranged perpendicular to the shaft of the detection rotating motor 618 and the top sheet flip rod 613. Specifically, the top sheet flip rod 613 and the shaft of the detection rotating motor 618 are parallel to each other, so that the rotation radius of the shaft of the detection rotating motor 618 is increased, thereby enabling the top sheet flip rod 613 to stably flip the liquid detection top sheet 616.

[0052] Further, see Figure 6 The top sheet flip rod 613 is provided with a top sheet engaging groove 602, and the liquid detection top sheet 616 is inserted into the top sheet engaging groove 602. In this embodiment, the top sheet engaging groove 602 is located on the top sheet flip rod 613, and the notch of the top sheet engaging groove 602 faces the liquid detection top sheet 616. A portion of the liquid detection top sheet 616 is engaged with the top sheet flip rod 613, so that the liquid detection top sheet 616 is stably engaged with the top sheet flip rod 613, thereby improving the connection stability between the liquid detection top sheet 616 and the top sheet flip rod 613.

[0053] In one embodiment, see Figure 6The tube liquid concentration detection component 610 also includes a near rod stop block 615 and a far rod stop block 617. The near rod stop block 615 and the far rod stop block 617 are both arranged on the liquid detection base 612. The near rod stop block 615 is located on the side of the rotating shaft extension rod 611 away from the rotating shaft of the detection rotating motor 618, and the near rod stop block 615 is used to abut against the rotating shaft extension rod 611. The far rod stop block 617 is located on the side of the top plate flip rod 613 away from the rotating shaft of the detection rotating motor 618, and the far rod stop block 617 is used to abut against the top plate flip rod 613. In this embodiment, the near-rod stopper 615 corresponds to the rotating shaft extension rod 611, and the far-rod stopper 617 corresponds to the top-sheet flipping rod 613. The near-rod stopper 615 serves as a flipping angle limiting component for the rotating shaft extension rod 611, while the far-rod stopper 617 serves as a flipping angle limiting component for the top-sheet flipping rod 613. When the liquid detection top sheet 616 is flipped open, the near-rod stopper 615 limits the flipping angle of the rotating shaft extension rod 611 to a specified angle, while the far-rod stopper 617 also limits the flipping angle of the top-sheet flipping rod 613 to the same specified angle. Specifically, the near-rod stopper 615 and the far-rod stopper 617 are arranged parallel to each other, thereby stabilizing the opening angle of the liquid detection top sheet 616 and preventing the liquid detection top sheet 616 from being damaged due to excessive flipping.

[0054] In one embodiment, see Figure 6The lens liquid cleaner 624 includes a liquid cleaning fixed plate 6242, a tape feed wheel 6244, a tape take-up wheel 6246 and a liquid cleaning extrusion head 6248. The liquid cleaning fixed plate 6242 is arranged on the lens liquid cleaning rack 622. The tape feed wheel 6244, the tape take-up wheel 6246 and the liquid cleaning extrusion head 6248 are all arranged on the liquid cleaning fixed plate 6242. The tape feed wheel 6244 is used to unwind the liquid absorbing tape, and the tape take-up wheel 6246 is used to rewind the liquid absorbing tape. The liquid cleaning extrusion head 6248 is located between the tape feed wheel 6244 and the tape take-up wheel 6246. The liquid cleaning extrusion head 6248 is used to squeeze the liquid absorbing tape onto the liquid detection substrate 614. In this embodiment, the clear liquid fixing plate 6242 serves as a mounting and fixing component for the tape feed roller 6244, the tape take-up roller 6246, and the clear liquid extrusion head 6248. Specifically, the tape feed roller 6244, the tape take-up roller 6246, and the clear liquid extrusion head 6248 are all located on a side of the clear liquid fixing plate 6242 that is close to the liquid detection base 612. The tape feed roller 6244 and the tape take-up roller 6246 work in conjunction with each other, with the tape feed roller 6244 delivering the wicking tape while the tape take-up roller 6246 simultaneously rewinds and recovers the wicking tape. The clear liquid extrusion head 6248 squeezes the wicking tape between the tape feed roller 6244 and the tape take-up roller 6246 onto the liquid detection substrate 614, facilitating the wiping of residual liquid from the liquid detection substrate 614 as the wicking tape reels.

[0055] Further, see Figure 7 The DNA tube liquid concentration testing assembly 600 also includes a clear liquid positioning component 630, and the clear liquid positioning component 630 includes a clear liquid positioning motor 632, a clear liquid positioning drive plate 634 and a clear liquid positioning movable rail 636. The clear liquid positioning motor 632 is arranged on the lens clear liquid rack 622, and the clear liquid positioning drive plate 634 is respectively connected to the telescopic shaft of the clear liquid positioning motor 632 and the clear liquid fixed plate 6242. The clear liquid positioning movable rail 636 is arranged on the clear liquid positioning drive plate 634, and the clear liquid positioning motor 632 slides on the clear liquid positioning movable rail 636. In this embodiment, the clear liquid position adjustment motor 632 serves as a position adjustment mechanism for the clear liquid extrusion head 6248. The clear liquid position adjustment motor 632 drives the clear liquid position adjustment drive plate 634. The clear liquid position adjustment motor 632 moves the clear liquid fixed plate 6242 via the clear liquid position adjustment drive plate 634, so that the clear liquid extrusion head 6248 on the clear liquid fixed plate 6242 moves closer to or further away from the liquid detection substrate 614. The clear liquid position adjustment movable rail 636 serves as a component for the clear liquid position adjustment drive plate 634 to slide on the clear liquid position adjustment motor 632, facilitating smooth movement of the clear liquid position adjustment drive plate 634.

[0056] In another embodiment, see Figure 8 The DNA tube liquid concentration testing component 600 also includes a clear liquid swinging member 640, and the clear liquid swinging member 640 includes a clear liquid swinging motor 642, a clear liquid swinging gear rod 644 and a clear liquid swinging gear 646. The clear liquid swinging motor 642 is fixed on the clear liquid fixed plate 6242, and the telescopic shaft of the clear liquid swinging motor 642 is connected to the clear liquid swinging gear rod 644, and the clear liquid swinging gear rod 644 is engaged with the clear liquid swinging gear 646. The clear liquid swinging gear 646 is rotatably set on the clear liquid fixed plate 6242, and the central axis of the clear liquid swinging gear 646 is connected to the clear liquid extrusion head 6248. In this embodiment, the clear liquid swing motor 642 serves as the power source of the clear liquid swing gear rod 644. The clear liquid swing motor 642 is installed on the clear liquid fixed plate 6242. The clear liquid swing gear rod 644 and the clear liquid swing gear 646 are meshed and connected with each other. When the clear liquid swing motor 642 extends and retracts the clear liquid swing gear rod 644, the clear liquid swing gear rod 644 pushes the clear liquid swing gear 646 to rotate. The clear liquid extrusion head 6248 is connected to the central axis of the clear liquid swing gear 646, so that the clear liquid swing gear 646 drives the clear liquid extrusion head 6248 to swing, so that the clear liquid extrusion head 6248 squeezes the liquid absorption belt onto the liquid detection substrate 614. Moreover, the swing of the clear liquid extrusion head 6248 enables the liquid absorption belt to swing and wipe the residual tube liquid on the liquid detection substrate 614.

[0057] For further information, see Figure 6The DNA tube liquid concentration testing component 600 also includes a clear liquid belt positioning component 650, which includes a belt movable connecting plate 652, a first telescopic cylinder 654, a second telescopic cylinder 656 and a plurality of belt conveying rods 658. The belt movable connecting plate 652 is connected to the central axis of the clear liquid swing gear 646. The first telescopic cylinder 654 is arranged on the side of the belt movable connecting plate 652 away from the clear liquid swing gear 646. The longitudinal moving end of the first telescopic cylinder 654 is connected to the second telescopic cylinder 656, and the lateral moving end of the second telescopic cylinder 656 is connected to the clear liquid extrusion head 6248. A plurality of belt conveying rods 658 are arranged around the edge of the belt movable connecting plate 652. The belt conveying rods 658 are used to slide against the liquid absorption belt to transmit the liquid absorption belt through the end of the clear liquid extrusion head 6248. In this embodiment, the belt-operated connecting plate 652 serves as an intermediate plate between the liquid-clearing swinging gear 646 and the first telescopic cylinder 654. The rotation of the liquid-clearing swinging gear 646 is transmitted to the first telescopic cylinder 654 via the belt-operated connecting plate 652, thereby facilitating the swinging of the liquid-clearing extrusion head 6248. The first telescopic cylinder 654 and the second telescopic cylinder 656 provide the longitudinal motion power for the liquid-clearing extrusion head 6248, enabling the liquid-clearing extrusion head 6248 to move in multiple directions. This facilitates adjustment of the relative position between the liquid-clearing extrusion head 6248 and the liquid detection substrate 614, ensuring precise alignment of the liquid-clearing extrusion head 6248 with the liquid detection substrate 614 during wiping. Multiple belt-operated conveying rods 658 collectively drive the wicking belt, assisting in its transport and ensuring smoother delivery to the end of the liquid-clearing extrusion head 6248.

[0058] In another embodiment, the clear liquid extrusion head 6248 is an elastic wiping head. Specifically, the clear liquid extrusion head 6248 has a built-in spring as a buffer to reduce the contact between the liquid suction belt and the end of the clear liquid extrusion head 6248.

[0059] In another embodiment, see Figure 6 The liquid belt positioning member 650 further includes at least one belt retaining ring 651, which is sleeved onto the belt conveying rod 658 and is configured to slide against the side of the absorbent belt facing away from the belt movable connecting plate 652. In this embodiment, the belt retaining ring 651 is sleeved onto the belt conveying rod 658 and serves as a position-limiting component for the absorbent belt during transport, thereby confining the absorbent belt to the belt conveying rod 658 and preventing the absorbent belt from detaching from the belt conveying rod 658 during transport.

[0060] In another embodiment, see Figure 7 The clear liquid positioning component 630 also includes a positioning sliding block 638, and the clear liquid positioning drive plate 634 has a U-shaped structure. The clear liquid positioning drive plate 634 includes a first positioning part 6342 and a second positioning part 6344 that are vertically connected to each other. The first positioning part 6342 is connected to the telescopic shaft of the clear liquid positioning motor 632, and the first positioning part 6342 is located on the side of the clear liquid positioning motor 632 away from the clear liquid fixed plate 6242. The second positioning part 6344 is also connected to the clear liquid fixed plate 6242. The clear liquid positioning movable rail 636 is located on the side of the second positioning part 6344 close to the clear liquid positioning motor 632. The positioning sliding block 638 is connected to the clear liquid positioning motor 632, and the positioning sliding block 638 is slidably set on the clear liquid positioning movable rail 636. In this embodiment, the first positioning portion 6342 and the second positioning portion 6344 form a U-shaped structure. Specifically, the two ends of a first positioning portion 6342 are respectively connected to a second positioning portion 6344, and the two second positioning portions 6344 are parallel to the telescopic axis of the clear liquid positioning motor 632. The positioning sliding block 638 is slidably connected to the clear liquid positioning movable rail 636 located on the second positioning portion 6344, so that when the second positioning portion 6344 drives the clear liquid fixing plate 6242 to extend or retract, the second positioning portion 6344 moves more smoothly relative to the clear liquid positioning motor 632. For example, the clear liquid positioning movable rail 636 and the telescopic axis of the clear liquid positioning motor 632 are parallel to each other. Moreover, the U-shaped structure formed by the first adjustment portion 6342 and the second adjustment portion 6344 reduces the distance between the clear liquid adjustment motor 632 and the clear liquid fixing plate 6242 , thereby reducing the space occupied by the clear liquid adjustment member 630 .

[0061] In another embodiment, see Figure 9The DNA tube liquid ultra-trace testing device 10B also includes a DNA test tube cover lifting assembly 700, which includes a DNA test tube mounting base 710, a cover lifting motor 720, and a cover lifting rotating rod 730. The DNA test tube mounting base 710 is arranged adjacent to the DNA test tube placement seat 500. The DNA test tube mounting base 710 is used to place the capped tubes to be tested that are transferred from the DNA test tube placement seat 500. The cover lifting motor 720 is connected to the DNA test tube mounting base 710, and the rotating shaft of the cover lifting motor 720 is connected to the cover lifting rotating rod 730. The cover lifting rotating rod 730 abuts against the inner side of the tube cap of the tube to be tested. In this embodiment, the DNA test tube mounting base 710 serves as a placement area for the capped tubes to be tested that are transferred from the DNA test tube placement base 500. For example, the tube liquid ultra-micro testing device further includes a test tube transport assembly, which is used to transport the capped tubes to be tested from the DNA test tube placement base 500 to the DNA test tube mounting base 710. At this time, the tube caps of the test tubes on the DNA test tube mounting base 710 are in a closed state. The cap lifting motor 720 is fixed to the DNA test tube mounting base 710. The cap lifting motor 720 serves as the turning power source of the cap lifting rotating rod 730. The rotating shaft of the cap lifting motor 720 drives the cap lifting rotating rod 730 to rotate, so that the cap lifting rotating rod 730 flips up the tube cap of the tube to be tested to open the cap of the tube to be tested, thereby facilitating the extraction of the tube liquid in the tube to be tested.

[0062] In another embodiment, see Figure 9 The DNA test tube mounting base 710 defines a test tube mounting groove 702, which receives a portion of the test tube. In this embodiment, the test tube mounting groove 702 is located on the DNA test tube mounting base 710 and receives a portion of the test tube. Specifically, the test tube mounting groove 702 serves as a placement location for the test tube, allowing the test tube to be stably placed on the DNA test tube mounting base 710. Specifically, at least a portion of the test tube is located within the test tube mounting groove 702, while the tube cap of the test tube is located outside the test tube mounting groove 702.

[0063] In another embodiment, see Figure 9The lid-lifting rotating lever 730 includes a first lid-lifting rotating portion 732 and a second lid-lifting rotating portion 734, which are perpendicularly connected to each other. The first lid-lifting rotating portion 732 is also connected to the rotating shaft of the lid-lifting motor 720. The second lid-lifting rotating portion 734 is arranged parallel to the rotating shaft of the lid-lifting motor 720. The second lid-lifting rotating portion 734 is configured to abut the inner side of the tube cap of the tube to be tested during the lid opening operation to open the tube cap. In this embodiment, the first lid-lifting rotating portion 732 serves as an extension of the rotating shaft of the lid-lifting motor 720. The rotation of the first lid-lifting rotating portion 732 is transmitted to the second lid-lifting rotating portion 734, causing the rotation center of the second lid-lifting rotating portion 734 to shift from the rotating shaft of the lid-lifting motor 720 to the connection with the first lid-lifting rotating portion 732. This increases the length of the second lid-lifting rotating portion 734's tilting arm, facilitating rapid lid opening of the tube to be tested by the second lid-lifting rotating portion 734. Moreover, when opening the cover, the second cover-lifting rotating portion 734 always abuts against the inner side of the tube cover of the tube to be tested, and the maximum distance between the second cover-lifting rotating portion 734 and the DNA test tube mounting base 710 is less than or equal to the maximum distance between the tube cover of the tube to be tested and the DNA test tube mounting base 710.

[0064] In another embodiment, see Figure 9 The DNA tube liquid ultra-micro test device 10B further includes a DNA test tube capping assembly 800, which includes a capping and retracting motor 810 and a capping and translation plate 820. The capping and retracting motor 810 is disposed on the DNA test tube mounting base 710, and the capping and translation plate 820 is connected to the retractable shaft of the capping and retracting motor 810. The capping and translation plate 820 is used to abut against the outer side of the tube cap of the test tube when closing the cap, thereby closing the cap of the test tube. In this embodiment, the capping and retracting motor 810 is fixed to the DNA test tube mounting base 710. Specifically, the capping and retracting motor 810 is fixed to an end of the DNA test tube mounting base 710 near the outer side of the tube cap of the test tube, and the capping and translation plate 820 is located between the capping and retracting motor 810 and the test tube. In this way, the cover retracting motor 810 pushes the cover translation plate 820 toward the outside of the tube cap of the tube to be tested, so that the cover translation plate 820 abuts the outside of the tube cap of the tube to be tested, facilitating the closing of the tube cap. Furthermore, when the cap is closed, the cover translation plate 820 always abuts the outside of the tube cap of the tube to be tested, and the distance between the bottom surface of the cover translation plate 820 and the DNA test tube mounting base 710 is greater than or equal to the distance between the outside of the tube cap of the tube to be tested and the DNA test tube mounting base 710 when the cap is closed.

[0065] In one embodiment, the present disclosure further provides a DNA test tube liquid calibration system, comprising the DNA test tube liquid concentration calibration device described in any of the above embodiments. In this embodiment, the DNA test tube liquid concentration calibration device comprises a DNA test tube liquid shaking device, a DNA tube liquid ultra-micro testing device, and a DNA tube liquid concentration calibration device; the DNA test tube liquid shaking device is used to shake the liquid in multiple DNA test tubes; the DNA tube liquid ultra-micro testing device is used to detect the concentration of the liquid in the DNA test tube; the DNA tube liquid concentration calibration device comprises a DNA tube liquid calibration mechanism and a DNA tube liquid dilution cup, the DNA tube liquid dilution cup is used to hold the dilution liquid, the DNA tube liquid calibration mechanism comprises a tube liquid calibration base, a tube liquid calibration robotic arm, and a tube liquid extraction The liquid calibration robot arm is arranged on the liquid calibration base, and the liquid extraction needle is arranged on the liquid calibration robot arm. The liquid extraction needle is used to extract the liquid in the DNA test tube to the test position of the DNA test tube ultra-micro test device to test the concentration of the liquid in the DNA test tube; the liquid extraction control end of the liquid extraction needle is connected to the liquid concentration adjustment end of the DNA test tube ultra-micro test device. The liquid extraction needle is also used to extract the dilution in the DNA test tube dilution cup into the DNA test tube when the concentration of the liquid in the DNA test tube is greater than the preset concentration, so as to make the concentration of the liquid in each DNA test tube equal. After the concentration test of the DNA test tube liquid, if the concentration of the DNA test tube liquid is too high, the liquid calibration robot arm drives the liquid extraction needle to the DNA test tube dilution cup to extract the dilution in the DNA test tube into the DNA test tube, and then repeats the above test and dilution operation until the concentration of the liquid in the DNA test tube reaches the preset concentration, so that the concentration of the liquid in each DNA test tube remains consistent.

[0066] The above-described embodiments merely represent several implementation methods of the present disclosure. While the descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the present invention. It should be noted that a person of ordinary skill in the art could make various modifications and improvements without departing from the spirit of the present disclosure, all of which fall within the scope of protection of the present disclosure. Therefore, the scope of protection of the present patent shall be determined by the appended claims.

Claims

1. A DNA test tube liquid concentration adjustment device, characterized in that: include: A DNA test tube liquid shaking device, which is used to shake the liquid in multiple DNA test tubes; DNA tube liquid ultra-micro testing device, the DNA tube liquid ultra-micro testing device is used to detect the concentration of the liquid in the DNA test tube; A DNA tube liquid concentration adjustment device, the DNA tube liquid concentration adjustment device includes a DNA tube liquid adjustment mechanism and a DNA tube liquid dilution cup, the DNA tube liquid dilution cup is used to hold the diluent, the DNA tube liquid adjustment mechanism includes a tube liquid adjustment base, a tube liquid adjustment mechanical arm and a tube liquid extraction needle, the tube liquid adjustment mechanical arm is arranged on the tube liquid adjustment base, the tube liquid extraction needle is arranged on the tube liquid adjustment mechanical arm, the tube liquid extraction needle is used to extract the liquid in the DNA test tube to the test position of the DNA tube liquid ultra-trace testing device to test the concentration of the liquid in the DNA test tube; the liquid collection control end of the tube liquid extraction needle is connected to the tube liquid concentration adjustment end of the DNA tube liquid ultra-trace testing device, and the tube liquid extraction needle is also used to extract the diluent in the DNA tube liquid dilution cup to the DNA test tube when the concentration of the liquid in the DNA test tube is greater than the preset concentration, so as to make the concentration of the liquid in each DNA test tube equal.

2. The DNA test tube liquid concentration adjustment device according to claim 1, characterized in that: The DNA tube liquid adjustment mechanism also includes a liquid collection slide rail, which is installed on the tube liquid adjustment mechanical arm, and the tube liquid extraction needle is slidably connected to the liquid collection slide rail.

3. The DNA test tube liquid concentration adjustment device according to claim 2, characterized in that: The DNA tube liquid adjustment mechanism further comprises a sampling slider, which is slidably arranged on the liquid collection slide rail, and the tube liquid extraction needle is fixed on the sampling slider.

4. The DNA test tube liquid concentration adjustment device according to claim 1, characterized in that: The DNA tube liquid adjustment mechanism further includes a liquid level probe, which is arranged on the tube liquid adjustment mechanical arm and is used to detect the liquid level of the liquid in the DNA test tube.

5. The DNA test tube liquid concentration adjustment device according to claim 1, characterized in that: The DNA tube liquid adjustment mechanism also includes an ultrasonic liquid level detector, which is installed on the tube liquid adjustment mechanical arm and is used to recheck the liquid level of the liquid in the DNA test tube.

6. The DNA test tube liquid concentration adjustment device according to claim 1, characterized in that: The DNA tube liquid adjustment mechanism also includes a test tube grabbing cylinder, which is fixedly connected to the tube liquid adjustment mechanical arm and is used to grab the DNA test tube.

7. The DNA test tube liquid concentration adjustment device according to claim 1, characterized in that: The DNA test tube liquid shaking device includes a DNA liquid shaking component and a DNA test tube locking component, the DNA liquid shaking component includes an oscillating polarization motor and a polarization plate, the rotating shaft of the oscillating polarization motor is connected to the polarization plate to make the polarization plate rotate eccentrically; the DNA test tube locking component includes a DNA test tube locking seat and a DNA test tube locking piece, the DNA test tube locking seat is connected to the side of the polarization plate away from the oscillating polarization motor, the DNA test tube locking seat has at least one test tube installation area, the test tube installation area is used to accommodate a variety of test tube assembly fixtures, and the test tube assembly fixture is used to hold DNA liquid; the DNA The test tube locking piece is arranged on the DNA test tube locking seat, and the DNA test tube locking piece is also used to connect with the test tube assembly fixture to install the test tube assembly fixture on the DNA test tube locking seat; wherein, the DNA liquid oscillation component also includes an oscillation positioning plate and a laser sensor, the oscillation positioning plate is mounted on the rotating shaft of the oscillation polarization motor, the oscillation positioning plate is provided with a positioning window, the laser sensor is arranged adjacent to the oscillation positioning plate, and the output end of the laser sensor is used to connect with the central control end of the oscillation polarization motor, so that when the oscillation polarization motor is started or reset, the sensing end of the laser sensor is arranged opposite to the positioning window.

8. The DNA test tube liquid concentration adjustment device according to claim 1, characterized in that: The DNA tube liquid ultra-trace testing device includes a DNA test tube placement seat and a DNA tube liquid concentration testing assembly, the DNA test tube placement seat is used to place DNA test tubes in batches; the DNA tube liquid concentration testing assembly includes a tube liquid concentration detection component and a lens liquid detection component, the tube liquid concentration detection component includes a liquid detection base, a liquid detection substrate, a liquid detection top plate and a detection rotating motor, the liquid detection substrate is arranged on the liquid detection base, the liquid detection top plate is rotatably connected to the liquid detection substrate, the detection rotating motor is connected to the liquid detection base, and the rotating shaft of the detection rotating motor is connected to the liquid detection top plate, so that the liquid detection top plate is flipped to fit the liquid detection substrate during the tube liquid test; the lens liquid detection component includes a lens liquid cleaning rack and a lens liquid cleaner, the lens liquid cleaning rack is arranged adjacent to the liquid detection base, the lens liquid cleaner is slidably arranged on the lens liquid cleaning rack, and the wiping end of the lens liquid cleaner is used to swing and wipe the liquid detection substrate.

9. The DNA test tube liquid concentration adjustment device according to claim 8, characterized in that: The lens liquid cleaner includes a liquid cleaning fixed plate, a tape feed wheel, a tape take-up wheel and a liquid cleaning extrusion head, the liquid cleaning fixed plate is arranged on the lens liquid cleaning rack, the tape feed wheel, the tape take-up wheel and the liquid cleaning extrusion head are all arranged on the liquid cleaning fixed plate, the tape feed wheel is used to unwind the liquid suction tape, the tape take-up wheel is used to rewind the liquid suction tape, the liquid cleaning extrusion head is located between the tape feed wheel and the tape take-up wheel, and the liquid cleaning extrusion head is used to squeeze the liquid suction tape onto the liquid detection substrate; the DNA tube liquid concentration test assembly also includes a liquid cleaning position adjustment part and a liquid cleaning swing part, the liquid cleaning position adjustment part includes a liquid cleaning position adjustment motor, a liquid cleaning position adjustment drive plate and a liquid cleaning position adjustment movable rail, the liquid cleaning position adjustment motor is arranged On the lens clear liquid rack, the clear liquid positioning drive plate is respectively connected to the telescopic shaft of the clear liquid positioning motor and the clear liquid fixed plate, the clear liquid positioning movable rail is arranged on the clear liquid positioning drive plate, and the clear liquid positioning motor slides on the clear liquid positioning movable rail; the clear liquid swinging part includes a clear liquid swinging motor, a clear liquid swinging gear rod and a clear liquid swinging gear, the clear liquid swinging motor is fixed on the clear liquid fixed plate, the telescopic shaft of the clear liquid swinging motor is connected to the clear liquid swinging gear rod, the clear liquid swinging gear rod is meshed with the clear liquid swinging gear, the clear liquid swinging gear is rotatably arranged on the clear liquid fixed plate, and the central axis of the clear liquid swinging gear is connected to the clear liquid extrusion head.

10. A DNA test tube liquid calibration system, characterized in that: It comprises the DNA test tube liquid concentration adjustment device as described in any one of claims 1 to 9.

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