DNA tube liquid concentration adjusting device and DNA tube liquid adjusting system

Abstract

The present disclosure provides a DNA test tube liquid concentration adjusting device and a DNA test tube liquid adjusting system. The device comprises a DNA test tube liquid shaking device, a DNA tube liquid ultra-micro testing device and a DNA tube liquid concentration adjusting device; the DNA tube liquid concentration adjusting device comprises a DNA tube liquid adjusting mechanism and a DNA tube liquid dilution cup, and the DNA tube liquid adjusting mechanism comprises a tube liquid adjusting base, a tube liquid adjusting mechanical arm and a tube liquid extraction needle; the tube liquid extraction needle is used to extract dilution liquid in the DNA tube liquid dilution cup to the DNA test tube when the concentration of the liquid in the DNA test tube is greater than a preset concentration, so that the concentration of the liquid in each DNA test tube is equal. The tube liquid adjusting mechanical arm drives the tube liquid extraction needle to the DNA tube liquid dilution cup, extracts the dilution liquid in the DNA tube liquid dilution cup to the DNA test tube, and then repeats the above testing and dilution operation until the concentration of the liquid in the DNA test tube reaches a predetermined concentration.

Description

TECHNICAL FIELD

[0001] The present disclosure relates to the technical field of DNA liquid testing, in particular to a DNA test tube liquid concentration adjusting device and a DNA test tube liquid adjusting system. BACKGROUND

[0002] At present, plasmid DNA is a common gene carrier in the field of genetic engineering technology and is widely used in the operation of constructing cloned cells. Extraction and purification of plasmid DNA is one of the most basic steps in molecular biology. Alkali lysis method is a classic method for extracting plasmid from cells, and most of the commercially available plasmid extraction kits adopt alkali lysis method. Plasmid miniprep is a common method for extracting experimental plasmid DNA, and many existing plasmid miniprep kits use centrifugal precipitation method. Compared with phenol extraction method, the reagents used in this method are less harmful to human body, and the operation is relatively simple. However, white flocculent precipitate will be produced during the experimental process of alkali lysis method, which generally needs to be removed by centrifugation, and the supernatant is separated to obtain plasmid DNA.

[0003] The above extraction method can only meet the small dose use of the laboratory, and the production line needs to transfer the precipitate mixed liquid to a centrifugal tube first, then high-speed centrifugation is performed to concentrate the precipitate at the bottom of the centrifugal tube, and then the supernatant is carefully sucked or poured out for production. However, the DNA liquid concentration in the centrifugal tube is usually higher than the standard concentration, that is, greater than the required qualified concentration, which easily leads to the inconsistency of the DNA liquid concentration in the centrifugal tube, which will greatly affect the subsequent use of the same batch of DNA liquid and cannot ensure the reliability of the experimental results. SUMMARY

[0004] The purpose of the present disclosure is to overcome the deficiencies in the prior art and provide a DNA test tube liquid concentration adjusting device and a DNA test tube liquid adjusting system which can effectively improve the consistency of DNA liquid concentration.

[0005] The purpose of the present disclosure is achieved by the following technical solutions:

[0006] The application discloses a DNA test tube liquid concentration adjusting device, which comprises a DNA test tube liquid shaking device, a DNA tube liquid ultramicro testing device and a DNA tube liquid concentration adjusting device; the DNA test tube liquid shaking device is used for shaking the liquid in a plurality of DNA test tubes; the DNA tube liquid ultramicro testing device is used for detecting the concentration of the liquid in the DNA test tubes; the DNA tube liquid concentration adjusting device comprises a DNA tube liquid adjusting mechanism and a DNA tube liquid dilution cup, the DNA tube liquid dilution cup is used for containing dilution liquid, the DNA tube liquid adjusting mechanism comprises a tube liquid adjusting base, a tube liquid adjusting mechanical arm and a tube liquid extraction needle, the tube liquid adjusting mechanical arm is arranged on the tube liquid adjusting base, the tube liquid extraction needle is arranged on the tube liquid adjusting mechanical arm, the tube liquid extraction needle is used for extracting the liquid in the DNA test tubes to a testing position of the DNA tube liquid ultramicro testing device, so as to test the concentration of the liquid in the DNA test tubes; the liquid extraction control end of the tube liquid extraction needle is connected with the tube liquid concentration adjusting end of the DNA tube liquid ultramicro testing device, and the tube liquid extraction needle is also used for extracting the dilution liquid in the DNA tube liquid dilution cup to the DNA test tubes when the concentration of the liquid in the DNA test tubes is greater than a preset concentration, so that the concentrations of the liquids in the DNA test tubes are equal.

[0007] In one of the embodiments, the DNA tube liquid adjusting mechanism further comprises a liquid extraction sliding rail, the liquid extraction sliding rail is mounted on the tube liquid adjusting mechanical arm, and the tube liquid extraction needle is slidably connected with the liquid extraction sliding rail.

[0008] In one of the embodiments, the DNA tube liquid adjusting mechanism further comprises a sampling sliding block, the sampling sliding block is slidably arranged on the liquid extraction sliding rail, and the tube liquid extraction needle is fixed on the sampling sliding block.

[0009] In one of the embodiments, the DNA tube liquid adjusting mechanism further comprises a liquid level probe, the liquid level probe is arranged on the tube liquid adjusting mechanical arm, and the liquid level probe is used for detecting the liquid level of the liquid in the DNA test tubes.

[0010] In one of the embodiments, the DNA tube liquid adjusting mechanism further comprises an ultrasonic liquid level detector, the ultrasonic liquid level detector is mounted on the tube liquid adjusting mechanical arm, and the ultrasonic liquid level detector is used for rechecking the liquid level of the liquid in the DNA test tubes.

[0011] In one of the embodiments, the DNA tube liquid adjusting mechanism further comprises a test tube grabbing cylinder, the test tube grabbing cylinder is fixedly connected with the tube liquid adjusting mechanical arm, and the test tube grabbing cylinder is used for grabbing the DNA test tubes.

[0012] In one embodiment, the DNA test tube liquid shaking device comprises a DNA liquid shaking assembly and a DNA test tube locking assembly. The DNA liquid shaking assembly comprises a shaking and polarizing motor and a polarizing plate. The shaft of the shaking and polarizing motor is connected with the polarizing plate to make the polarizing plate eccentrically rotate. The DNA test tube locking assembly comprises a DNA test tube locking seat and a DNA test tube locking piece. The DNA test tube locking seat is connected with the side of the polarizing plate away from the shaking and polarizing motor. The DNA test tube locking seat has at least one test tube mounting area for accommodating various test tube assembly clamps for containing DNA liquid. The DNA test tube locking piece is arranged on the DNA test tube locking seat and is used to connect with the test tube assembly clamps to mount the test tube assembly clamps on the DNA test tube locking seat. The DNA liquid shaking assembly further comprises a shaking positioning disc and a laser sensor. The shaking positioning disc is sleeved on the shaft of the shaking and polarizing motor. The shaking positioning disc is provided with a positioning window. The laser sensor is arranged adjacent to the shaking positioning disc. The output end of the laser sensor is connected with the control end of the shaking and polarizing motor to make the sensing end of the laser sensor oppositely arranged with the positioning window when the shaking and polarizing motor is started or reset.

[0013] In one embodiment, the DNA test tube liquid shaking device comprises a DNA liquid shaking assembly and a DNA test tube locking assembly. The DNA liquid shaking assembly comprises a shaking and polarizing motor and a polarizing plate. The shaft of the shaking and polarizing motor is connected with the polarizing plate to make the polarizing plate eccentrically rotate. The DNA test tube locking assembly comprises a DNA test tube locking seat and a DNA test tube locking piece. The DNA test tube locking seat is connected with the side of the polarizing plate away from the shaking and polarizing motor. The DNA test tube locking seat has at least one test tube mounting area for accommodating various test tube assembly clamps for containing DNA liquid. The DNA test tube locking piece is arranged on the DNA test tube locking seat and is used to connect with the test tube assembly clamps to mount the test tube assembly clamps on the DNA test tube locking seat. The DNA liquid shaking assembly further comprises a shaking positioning disc and a laser sensor. The shaking positioning disc is sleeved on the shaft of the shaking and polarizing motor. The shaking positioning disc is provided with a positioning window. The laser sensor is arranged adjacent to the shaking positioning disc. The output end of the laser sensor is connected with the control end of the shaking and polarizing motor to make the sensing end of the laser sensor oppositely arranged with the positioning window when the shaking and polarizing motor is started or reset.

[0014] In one of the embodiments, the lens cleaning device comprises a cleaning fixed plate, a feeding belt wheel, a winding belt wheel and a cleaning extrusion head, the cleaning fixed plate is arranged on the lens cleaning rack, the feeding belt wheel, the winding belt wheel and the cleaning extrusion head are arranged on the cleaning fixed plate, the feeding belt wheel is used for unwinding the liquid suction belt, the winding belt wheel is used for winding the liquid suction belt, the cleaning extrusion head is located between the feeding belt wheel and the winding belt wheel, and the cleaning extrusion head is used for extruding the liquid suction belt on the liquid detection substrate; the DNA tube liquid concentration testing assembly further comprises a cleaning positioner and a cleaning swing device, the cleaning positioner comprises a cleaning positioner motor, a cleaning positioner driving plate and a cleaning positioner moving rail, the cleaning positioner motor is arranged on the lens cleaning rack, the cleaning positioner driving plate is connected with the extension shaft of the cleaning positioner motor and the cleaning fixed plate respectively, the cleaning positioner moving rail is arranged on the cleaning positioner driving plate, and the cleaning positioner motor slides on the cleaning positioner moving rail; the cleaning swing device comprises a cleaning swing motor, a cleaning swing toothed rod and a cleaning swing gear, the cleaning swing motor is fixed on the cleaning fixed plate, the extension shaft of the cleaning swing motor is connected with the cleaning swing toothed rod, the cleaning swing toothed rod is engaged with the cleaning swing gear, the cleaning swing gear is rotationally arranged on the cleaning fixed plate, and the central shaft of the cleaning swing gear is connected with the cleaning extrusion head.

[0015] The DNA test tube liquid concentration adjusting system comprises the DNA test tube liquid concentration adjusting device of any one of the embodiments.

[0016] Compared with the prior art, the present disclosure has at least the following advantages:

[0017] After the DNA test tube liquid concentration test, when the DNA test tube liquid concentration is too high, the tube liquid adjusting mechanical arm drives the tube liquid extraction needle to the DNA tube liquid dilution cup, extracts the dilution liquid in the DNA tube liquid dilution cup into the DNA test tube, and then repeats the above test and dilution operation until the concentration of the liquid in the DNA test tube reaches the predetermined concentration, so that the concentration of the liquid in each DNA test tube remains consistent. BRIEF DESCRIPTION OF DRAWINGS

[0018] In order to more clearly illustrate the technical solutions of the embodiments of the present disclosure, the following will briefly introduce the drawings needed to be used in the embodiments. It should be understood that the following drawings only show some embodiments of the present disclosure, and therefore should not be regarded as a limitation on the scope. For those skilled in the art, other related drawings can also be obtained without creative labor on the basis of these drawings.

[0019] Figure 1 It is a schematic diagram of the DNA test tube liquid concentration adjusting device in one embodiment;

[0020] Figure 2 A schematic diagram of a DNA tube liquid concentration adjustment device in one embodiment;

[0021] Figure 3 Schematic diagram of a DNA test tube liquid shaking device in one embodiment;

[0022] Figure 4 for Figure 3 A schematic diagram of the DNA test tube liquid shaking device from another perspective;

[0023] Figure 5 Schematic diagram of a DNA tube liquid ultra-micro test device in one embodiment;

[0024] Figure 6 A schematic diagram of a DNA tube liquid concentration test assembly in one embodiment;

[0025] Figure 7 for Figure 6 A schematic diagram of the DNA tube liquid concentration test assembly from another perspective is shown;

[0026] Figure 8 for Figure 6 A schematic diagram of the DNA tube liquid concentration test assembly from another perspective is shown;

[0027] Figure 9 Schematic diagram of the combination of a DNA test tube cover lifting assembly and a DNA test tube cover buckling assembly in one embodiment. DETAILED DESCRIPTION

[0028] To facilitate understanding of the present disclosure, a more comprehensive description of the present disclosure will be provided below with reference to the accompanying drawings. The accompanying drawings illustrate preferred embodiments of the present disclosure. However, the present disclosure can be implemented in many different forms and is not limited to the embodiments described herein. Rather, these embodiments are provided to provide a more thorough and comprehensive understanding of the disclosure.

[0029] It should be noted that when an element is referred to as being "fixed to" another element, it may be directly attached to the other element or there may be an intermediate element. When an element is referred to as being "connected to" another element, it may be directly connected to the other element or there may be an intermediate element. The terms "vertical," "horizontal," "left," "right," and similar expressions used herein are for illustrative purposes only and do not represent the only implementation methods.

[0030] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this disclosure belongs. The terminology used in the description of the disclosure herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the disclosure. As used herein, the term "and / or" includes any and all combinations of one or more of the associated listed items.

[0031] The present disclosure relates to a DNA tube liquid concentration adjusting device. In one embodiment, the DNA tube liquid concentration adjusting device comprises a DNA tube liquid shaking device, a DNA tube liquid ultra-micro testing device, and a DNA tube liquid concentration adjusting device. The DNA tube liquid shaking device is used to shake the liquid in a plurality of DNA tubes. The DNA tube liquid ultra-micro testing device is used to detect the concentration of the liquid in the DNA tubes. The DNA tube liquid concentration adjusting device comprises a DNA tube liquid adjusting mechanism and a DNA tube liquid dilution cup. The DNA tube liquid dilution cup is used to hold dilution liquid. The DNA tube liquid adjusting mechanism comprises a tube liquid adjusting base, a tube liquid adjusting mechanical arm, and a tube liquid extraction needle. The tube liquid adjusting mechanical arm is arranged on the tube liquid adjusting base. The tube liquid extraction needle is arranged on the tube liquid adjusting mechanical arm. The tube liquid extraction needle is used to extract the liquid in the DNA tubes to the testing position of the DNA tube liquid ultra-micro testing device to test the concentration of the liquid in the DNA tubes. The liquid extraction control end of the tube liquid extraction needle is connected with the tube liquid concentration adjusting end of the DNA tube liquid ultra-micro testing device. The tube liquid extraction needle is also used to extract the dilution liquid in the DNA tube liquid dilution cup to the DNA tubes when the concentration of the liquid in the DNA tubes is greater than a preset concentration, so that the concentrations of the liquids in the DNA tubes are equal. After the concentration test of the DNA tube liquid, when the concentration of the DNA tube liquid is too high, the tube liquid adjusting mechanical arm drives the tube liquid extraction needle to the DNA tube liquid dilution cup to extract the dilution liquid in the DNA tube liquid dilution cup to the DNA tubes. Then the above-mentioned test and dilution operation are repeated until the concentration of the liquid in the DNA tubes reaches a predetermined concentration, so that the concentrations of the liquids in the DNA tubes remain consistent.

[0032] Please refer to Figure 1 which is a structural schematic diagram of the DNA tube liquid concentration adjusting device according to an embodiment of the present disclosure.

[0033] The DNA tube liquid concentration adjusting device 10 according to an embodiment comprises a DNA tube liquid shaking device 10A, a DNA tube liquid ultra-micro testing device 10B, and a DNA tube liquid concentration adjusting device 10C. The DNA tube liquid shaking device 10A is used to shake the liquid in a plurality of DNA tubes. The DNA tube liquid ultra-micro testing device 10B is used to detect the concentration of the liquid in the DNA tubes. Please refer to Figure 2The DNA liquid concentration adjustment device 10C includes a DNA liquid adjustment mechanism 100 and a DNA liquid dilution cup 200. The DNA liquid dilution cup 200 is used to hold the diluent. The DNA liquid adjustment mechanism 100 includes a liquid adjustment base 110, a liquid adjustment robot 120, and a liquid extraction needle 130. The liquid adjustment robot 120 is mounted on the liquid adjustment base 110. The liquid extraction needle 130 is mounted on the liquid adjustment robot 120. The liquid extraction needle 130 is used to extract the liquid in the DNA test tube to the testing position of the DNA liquid ultra-micro testing device 10B to test the concentration of the liquid in the DNA test tube. The liquid extraction control end of the tube liquid extraction needle 130 is connected to the tube liquid concentration adjustment end of the DNA tube liquid ultra-trace testing device 10B. The tube liquid extraction needle 130 is also used to extract the diluted liquid in the DNA tube liquid dilution cup 200 into the DNA test tube when the concentration of the liquid in the DNA test tube is greater than the preset concentration, so as to make the concentration of the liquid in each DNA test tube equal.

[0034] In this embodiment, after the concentration test of the DNA test tube liquid, if the concentration of the DNA test tube liquid is too high, the tube liquid adjustment robot 120 drives the tube liquid extraction needle 130 to the DNA tube liquid dilution cup 200 to extract the diluted liquid in the DNA tube liquid dilution cup 200 into the DNA test tube. Thereafter, the above-mentioned testing and dilution operations are repeated until the concentration of the liquid in the DNA test tube reaches the predetermined concentration, so that the concentration of the liquid in each DNA test tube remains consistent.

[0035] In another embodiment, the liquid extraction needle is a replaceable TIP needle, that is, the liquid extraction needle has multiple TIP needles connected in series. After each liquid extraction, the original TIP needle is automatically removed and a new TIP needle is used to extract the liquid to avoid cross contamination of the liquid.

[0036] In one embodiment, see Figure 2 The DNA tube liquid calibration mechanism 100 further includes a liquid collection slide 140, which is mounted on the tube liquid calibration robot arm 120. The tube liquid extraction needle 130 is slidably connected to the liquid collection slide 140. In this embodiment, the liquid collection slide 140 is located on the tube liquid calibration robot arm 120, and the tube liquid calibration robot arm 120 performs liquid collection operations through the tube liquid extraction needle 130. Specifically, the tube liquid calibration robot arm 120 is a robot arm with 4 or more degrees of freedom. The end of the tube liquid calibration robot arm 120 is connected to the liquid collection slide 140. The tube liquid extraction needle 130 slides along the direction of the liquid collection slide 140, which facilitates adjustment of the penetration depth of the tube liquid extraction needle 130 in the DNA test tube and the DNA tube liquid dilution cup 200, so that the DNA liquid dosage extracted by the tube liquid extraction needle 130 is more accurate.

[0037] Further, referring to Figure 2 , the DNA tube liquid adjusting mechanism 100 further comprises a sampling slider 150, the sampling slider 150 is slidingly arranged on the liquid taking slide rail 140, and the tube liquid extraction needle 130 is fixed on the sampling slider 150. In this embodiment, the sampling slider 150 is located between the liquid taking slide rail 140 and the tube liquid extraction needle 130, and the sampling slider 150 serves as the base for the tube liquid extraction needle 130 to slide on the liquid taking slide rail 140, that is, the tube liquid extraction needle 130 slides on the liquid taking slide rail 140 through the sampling slider 150. The sampling slider 150 acts as the sliding component of the tube liquid extraction needle 130, avoiding friction between the tube liquid extraction needle 130 and the liquid taking slide rail 140, thereby reducing the damage probability of the tube liquid extraction needle 130 and ensuring the stable and safe liquid taking of the tube liquid extraction needle 130.

[0038] In one embodiment, referring to Figure 2 , the DNA tube liquid adjusting mechanism 100 further comprises a liquid level probe 160, the liquid level probe 160 is arranged on the tube liquid adjusting mechanical arm 120, and the liquid level probe 160 is used to detect the liquid level in the DNA test tube. In this embodiment, the liquid level probe 160 is installed on the tube liquid adjusting mechanical arm 120, specifically, the tube liquid adjusting mechanical arm 120 has a liquid level detection area, the liquid level probe 160 is arranged in the liquid level detection area, and the liquid level detection area is separated from the area where the tube liquid extraction needle 130 is located, so as to avoid the liquid level probe 160 being too close to the tube liquid extraction needle 130, thereby avoiding the liquid splashing from the liquid level probe 160 to the tube liquid extraction needle 130 and causing the extraction liquid to be contaminated, thereby ensuring the accuracy of the concentration test of the DNA liquid extracted by the tube liquid extraction needle 130.

[0039] In one embodiment, referring to Figure 2The DNA tube liquid adjusting mechanism 100 further comprises an ultrasonic liquid level detector 170 installed on the tube liquid adjusting mechanical arm 120, which is used to recheck the liquid level in the DNA tube. In this embodiment, the ultrasonic liquid level detector 170 is located on the tube liquid adjusting mechanical arm 120, and specifically, the ultrasonic liquid level detector 170 is arranged adjacent to the liquid level detection area, so that the ultrasonic liquid level detector 170 is close to the liquid level probe 160. The ultrasonic liquid level detector 170 determines the liquid level in the DNA tube by ultrasonic positioning, and when the liquid level probe 160 detects the liquid level, the ultrasonic liquid level detector 170 synchronously detects the liquid level again, so as to calibrate the liquid level detected by the liquid level probe 160, thereby improving the detection accuracy of the liquid level in the DNA tube.

[0040] In one embodiment, referring to Figure 2 The DNA tube liquid adjusting mechanism 100 further comprises a tube grabbing cylinder 180 fixedly connected with the tube liquid adjusting mechanical arm 120, which is used to grab the DNA tube. In this embodiment, the tube grabbing cylinder 180 is arranged on the tube liquid adjusting mechanical arm 120, and the tube grabbing cylinder 180 serves as a grabbing component of the DNA tube, so as to facilitate the loading, unloading and transfer of the DNA tube, thereby facilitating the movement of the DNA tube to the corresponding station.

[0041] In one embodiment, referring to Figure 3 The DNA tube liquid shaking device 10A comprises a DNA liquid shaking assembly 300 and a DNA tube locking assembly 400. The DNA liquid shaking assembly 300 comprises a shaking polarization motor 310 and a polarization flat plate 320. The rotating shaft of the shaking polarization motor 310 is connected with the polarization flat plate 320, so that the polarization flat plate 320 rotates eccentrically. The DNA tube locking assembly 400 comprises a DNA tube locking seat 410 and a DNA tube locking piece 420. The DNA tube locking seat 410 is connected with the side of the polarization flat plate 320 away from the shaking polarization motor 310. The DNA tube locking seat 410 has at least one tube mounting area for accommodating a plurality of tube assembly clamps for containing DNA liquid. The DNA tube locking piece 420 is arranged on the DNA tube locking seat 410 and is used to connect with the tube assembly clamps to mount the tube assembly clamps on the DNA tube locking seat 410.

[0042] In the embodiment, the DNA test tube locking seat 410 is used to place various types of test tube assembly clamps, the DNA test tube locking piece 420 is used to fix various types of test tube assembly clamps on the DNA test tube locking seat 410, so as to improve the test tube type; the oscillation polarization motor 310 drives the polarization flat plate 320 to shake, after the test tube assembly clamp is stabilized on the DNA test tube locking seat 410, the oscillation polarization motor 310 shakes the DNA liquid in the test tube on the test tube assembly clamp in batches and quickly, which effectively reduces the production cost of the test tube liquid shaking.

[0043] In one embodiment, referring to Figure 3 , the DNA test tube locking seat 410 is provided with a plurality of test tube locking grooves 402, and the test tube locking grooves 402 are used to clamp part of the test tube assembly clamp. In the embodiment, the test tube locking groove 402 is located on the DNA test tube locking seat 410, and the test tube locking groove 402 is used as a fixed mounting groove of the test tube assembly clamp. Specifically, the test tube locking groove 402 is located in the test tube mounting area, and part of the test tube assembly clamp is accommodated in the test tube locking groove 402. The mounting position formed by the test tube locking groove 402 facilitates embedding the test tube assembly clamp in the DNA test tube locking seat 410, so as to improve the mounting stability of the test tube assembly clamp on the DNA test tube locking seat 410.

[0044] Further, a plurality of test tube locking grooves 402 are arranged in parallel with each other. In the embodiment, the test tube locking groove 402 fixes the test tube assembly clamp on the DNA test tube locking seat 410, and a plurality of test tube locking grooves 402 are kept parallel, so that a plurality of test tube assembly clamps are kept parallel at the same time, thereby increasing the number of test tube assembly clamps on the DNA test tube locking seat 410, and facilitating batch shaking of the test tubes.

[0045] In another embodiment, the test tube locking groove 402 is a back-shaped through groove, and the upper part of the test tube locking groove 402 has an opening. The test tube assembly clamp has an I-shaped structure, so that the test tube assembly clamp is adapted to the test tube locking groove 402, and the test tube assembly clamp enters from the entrance of the test tube locking groove 402, so that the upper opening of the test tube locking groove 402 clamps the middle part of the I-shaped structure of the test tube assembly clamp.

[0046] In one embodiment, referring to Figure 3The DNA test tube locking member 420 comprises a locking outer plate 422 and a locking inner plate 424, the locking outer plate 422 is connected with the DNA test tube locking seat 410, the locking inner plate 424 is located between the locking outer plate 422 and the test tube assembly clamp, and the locking outer plate 422 and the locking inner plate 424 span the plurality of test tube locking grooves 402. In the embodiment, the locking inner plate 424 abuts against the locking outer plate 422 and the test tube assembly clamp respectively, and the locking inner plate 424 serves as a buffer plate fixedly extruded between the locking outer plate 422 and the test tube assembly clamp. The locking outer plate 422 and the locking inner plate 424 correspond to the upper openings of the test tube locking grooves 402, so that the locking outer plate 422 and the locking inner plate 424 limit the test tube assembly clamp in the test tube locking groove 402, thereby improving the mounting stability of the test tube assembly clamp and the stability of the test tube assembly clamp when shaken.

[0047] In another embodiment, the locking outer plate 422 and the locking inner plate 424 are adjacent to the mounting entrances of the test tube locking grooves 402. In the embodiment, the locking outer plate 422 and the locking inner plate 424 correspond to the mounting entrances of the test tube locking grooves 402, specifically, the locking outer plate 422 and the locking inner plate 424 are located above the test tube locking grooves 402 and close to the mounting entrances of the test tube locking grooves 402, so that the locking inner plate 424 abuts against the end of the test tube assembly clamp, thereby facilitating the limitation of the test tube assembly clamp in the test tube locking groove 402.

[0048] Further, referring to Figure 3 The DNA test tube locking member 420 further comprises a locking connecting rod 426, the locking connecting rod 426 is rotationally connected with the DNA test tube locking seat 410, and the locking connecting rod 426 is clamped with the locking outer plate 422. In the embodiment, the locking connecting rod 426 is rotationally arranged on the DNA test tube locking seat 410, specifically, one end of the locking connecting rod 426 is rotationally connected with the side wall of the DNA test tube locking seat 410, and the other end of the locking connecting rod 426 is clamped with the locking outer plate 422. The locking connecting rod 426 is connected with the locking outer plate 422 in a rotational manner, thereby facilitating the fixation of the locking outer plate 422 on the DNA test tube locking seat 410, and the rotational connection of the locking connecting rod 426 facilitates the disassembly and maintenance of the locking outer plate 422.

[0049] In another embodiment, referring to Figure 3The locking connecting rod 426 is provided with a locking buckle groove 404, and the groove of the locking buckle groove 404 is directed to the locking outer plate 422. The locking buckle groove 404 is used for accommodating part of the locking outer plate 422, so that part of the locking outer plate 422 is clamped in the locking buckle groove 404. In the embodiment, the groove of the locking buckle groove 404 is directed downward, and in particular, the groove of the locking buckle groove 404 is directed to the polarization flat plate 320, so that the locking connecting rod 426 is clamped on the locking outer plate 422 in an inverted manner.

[0050] In one embodiment, referring to Figure 3 The DNA tube locking piece 420 further comprises a rotationally connected locking lever 428 and a locking ring 421. The locking lever 428 is rotationally connected with the DNA tube locking seat 410. The connection point of the locking lever 428 and the DNA tube locking seat 410 is located between the rotationally connected point of the locking ring 421 and the locking lever 428 and the locking outer plate 422. The locking ring 421 is sleeved on the locking outer plate 422. In the embodiment, the locking lever 428 and the DNA tube locking seat 410 have one rotation point, and the locking lever 428 and the locking ring 421 have another rotation point. The locking ring 421 is also sleeved with the locking outer plate 422, that is, part of the locking outer plate 422 is located in the locking ring 421. The locking ring 421 is sleeved on the locking outer plate 422 by rotating the locking lever 428. When the locking lever 428 rotates relative to the DNA tube locking seat 410 and the end of the locking lever 428 is directed to move away from the locking outer plate 422, the locking ring 421 is driven by the locking lever 428 to move away from the locking outer plate 422, so that the locking ring 421 stably presses the locking outer plate 422 on the tube assembly clamp, thereby making the tube assembly clamp more stable on the DNA tube locking seat 410.

[0051] In another embodiment, the DNA tube locking piece is a bolt, and the tube assembly clamp is an integral kit which is provided with a plurality of liquid receiving grooves arranged in an array, so as to screw the tube assembly clamp on the DNA tube locking seat.

[0052] In one embodiment, referring to Figure 4The DNA liquid shock assembly 300 further comprises a shock positioning disc 330 and a laser sensor 340. The shock positioning disc 330 is sleeved on the rotating shaft of the shock polar motor 310 and is provided with a positioning window 302. The laser sensor 340 is arranged adjacent to the shock positioning disc 330. The output end of the laser sensor 340 is connected with the control end of the shock polar motor 310, so that the sensing end of the laser sensor 340 is arranged opposite to the positioning window 302 when the shock polar motor 310 is started or reset. In the embodiment, the shock positioning disc 330 is arranged on the rotating shaft of the shock polar motor 310. The shock positioning disc 330 deflects when the rotating shaft of the shock polar motor 310 rotates, so that the deflection degree of the shock positioning disc 330 corresponds to the shaking degree of the test tube assembly clamp. The sensing end of the laser sensor 340 is used in cooperation with the positioning window 302 on the shock positioning disc 330. When the sensing end of the laser sensor 340 is aligned with the positioning window 302 on the shock positioning disc 330, the shock polar motor 310 is in the initial state or the reset state, that is, the initial shaking position of the shock polar motor 310. At this time, the light emitted by the sensing end of the laser sensor 340 passes through the positioning window 302 and no reflected light is received. When the shock polar motor 310 is in the shaking state, the shock positioning disc 330 deflects, so that the light emitted by the sensing end of the laser sensor 340 deviates from the positioning window 302, that is, the light is reflected on the shock positioning disc 330, so that the reflected light is received. In this way, the laser sensor 340 determines and adjusts the working state of the shock polar motor 310 according to the received reflected light, so that the test tube on the test tube assembly clamp is placed stably after shaking.

[0053] In another embodiment, the laser sensor 340 is a reflection sensor, that is, the sensing end of the laser sensor 340 comprises a light receiving probe and a light emitting probe. The light receiving probe and the light emitting probe are arranged opposite to each other and correspond to the positioning window 302. In the initial or reset state, the light emitted by the light emitting probe is received by the light receiving probe. In the shaking state, the light emitted by the light emitting probe is blocked by the shock positioning disc 330 and the light receiving probe cannot receive the light.

[0054] In one of the embodiments, please refer to Figure 3The DNA test tube liquid shaking device 10A further comprises a DNA test tube code acquisition assembly 430, which comprises a test tube code acquisition device 432 and a code acquisition moving track 434, the code acquisition moving track 434 is arranged adjacent to the DNA test tube locking seat 410, and the test tube code acquisition device 432 is slidingly arranged on the code acquisition moving track 434, and the code acquisition head of the test tube code acquisition device 432 faces the DNA test tube locking seat 410 to acquire the code of each test tube assembly clamp. In the embodiment, the test tube code acquisition device 432 serves as an acquisition device of the code of each test tube assembly clamp, the test tube code acquisition device 432 moves along the direction in which the code acquisition moving track 434 is arranged, the code acquisition head of the test tube code acquisition device 432 faces the test tube assembly clamp, and when a plurality of test tube assembly clamps are sequentially arranged on the DNA test tube locking seat 410, the test tube code acquisition device 432 moves on the code acquisition moving track 434 step by step to respectively record the code of each test tube assembly clamp, so that the code information of each test tube assembly clamp is accurately acquired.

[0055] In another embodiment, the code acquisition moving track 434 is perpendicular to the test tube locking groove 402, so that after one test tube assembly clamp is arranged and the code acquisition head of the test tube code acquisition device 432 acquires the code of the test tube assembly clamp, the test tube code acquisition device 432 moves along the code acquisition moving track 434 by a predetermined distance and away from the test tube assembly clamp to scan the next arranged test tube assembly clamp.

[0056] In one of the embodiments, referring to Figure 3 The DNA test tube liquid shaking device 10A further comprises a test tube flatness detector 440, which is connected with the oscillation bias motor 310, and the probe of the test tube flatness detector 440 faces the test tube assembly clamp to detect the height of each test tube assembly clamp. In the embodiment, the test tube flatness detector 440 serves as a height detector of a plurality of test tube assembly clamps, and through the height measurement of each test tube assembly clamp, the arrangement and flatness of a plurality of test tube assembly clamps are checked, so that the arrangement of a plurality of test tube assembly clamps on the DNA test tube locking seat 410 is more stable, thereby facilitating the batch and synchronous shaking operation of test tube liquid.

[0057] In one of the embodiments, referring to Figure 5 The DNA test tube liquid shaking device 10A further comprises a DNA test tube liquid ultra-micro test device 10B, which comprises a DNA test tube placing seat 500 and a DNA test tube liquid concentration test assembly 600, the DNA test tube placing seat 500 is used for placing a plurality of DNA test tubes; and referring to Figure 6The DNA tube liquid concentration test assembly 600 comprises a tube liquid concentration detecting piece 610 and a detecting lens cleaning piece 620. The tube liquid concentration detecting piece 610 comprises a liquid detecting base 612, a liquid detecting substrate 614, a liquid detecting top sheet 616 and a detecting rotary motor 618. The liquid detecting substrate 614 is arranged on the liquid detecting base 612. The liquid detecting top sheet 616 is rotationally connected with the liquid detecting substrate 614. The detecting rotary motor 618 is connected with the liquid detecting base 612. The rotary shaft of the detecting rotary motor 618 is connected with the liquid detecting top sheet 616, so that the liquid detecting top sheet 616 is turned over to be attached with the liquid detecting substrate 614 during the tube liquid test. The detecting lens cleaning piece 620 comprises a lens cleaning frame 622 and a lens cleaning device 624. The lens cleaning frame 622 is arranged adjacent to the liquid detecting base 612. The lens cleaning device 624 is slidingly arranged on the lens cleaning frame 622. The wiping end of the lens cleaning device 624 is used to swing and wipe the liquid detecting substrate 614.

[0058] In the embodiment, before the tube liquid test, the liquid taking piece drops the tube liquid on the liquid detecting substrate 614. The detecting rotary motor 618 buckles the liquid detecting top sheet 616 on the liquid detecting substrate 614 to form a test sample chamber. After the test is completed, the detecting rotary motor 618 opens the liquid detecting top sheet 616. At this time, the lens cleaning device 624 moves to the liquid detecting substrate 614 to swing and wipe the residual tube liquid on the liquid detecting substrate 614, so that the tube liquid test efficiency is effectively improved.

[0059] In another embodiment, the tube liquid concentration detecting piece is an ultramicro spectrophotometer.

[0060] In one of the embodiments, please refer to Figure 6The tube liquid concentration detecting piece 610 further comprises a rotating shaft extension rod 611 and a top sheet turning rod 613 which are connected with each other. The rotating shaft extension rod 611 is connected with the rotating shaft of the detecting rotating motor 618. The top sheet turning rod 613 is located at the end of the rotating shaft extension rod 611 which is away from the rotating shaft of the detecting rotating motor 618. The top sheet turning rod 613 is connected with the side of the liquid detecting top sheet 616 which is away from the liquid detecting base sheet 614. In this embodiment, the rotating shaft extension rod 611 serves as a turning extension rod of the detecting rotating motor 618. The rotating shaft extension rod 611 rotates around the rotating shaft of the detecting rotating motor 618. The top sheet turning rod 613 serves as a turning component of the liquid detecting base sheet 614. The detecting rotating motor 618 drives the top sheet turning rod 613 to rotate through the rotating shaft extension rod 611. The top sheet turning rod 613 drives the liquid detecting top sheet 616 to rotate, so that the liquid detecting top sheet 616 moves away from or approaches the liquid detecting base sheet 614, facilitating the opening and closing operation of the liquid detecting top sheet 616 and the liquid detecting base sheet 614.

[0061] In another embodiment, the rotating shaft extension rod 611 is arranged perpendicularly to the rotating shaft of the detecting rotating motor 618 and the top sheet turning rod 613. Specifically, the top sheet turning rod 613 is parallel to the rotating shaft of the detecting rotating motor 618, so that the rotating radius of the rotating shaft of the detecting rotating motor 618 is increased, thereby stabilizing the turning of the top sheet turning rod 613 to the liquid detecting top sheet 616.

[0062] Further, referring to Figure 6 The top sheet turning rod 613 is provided with a top sheet clamping groove 602. The liquid detecting top sheet 616 is clamped in the top sheet clamping groove 602. In this embodiment, the top sheet clamping groove 602 is located on the top sheet turning rod 613. The groove opening of the top sheet clamping groove 602 faces the liquid detecting top sheet 616. Part of the liquid detecting top sheet 616 is clamped in the top sheet clamping groove 602, so that the liquid detecting top sheet 616 is stably clamped with the top sheet turning rod 613, thereby improving the connection stability between the liquid detecting top sheet 616 and the top sheet turning rod 613.

[0063] In one embodiment, referring to Figure 6The tube liquid concentration detection piece 610 further comprises a near-rod stop block 615 and a far-rod stop block 617, both of which are arranged on the liquid detection base 612. The near-rod stop block 615 is located on the side of the shaft extension rod 611 away from the shaft of the detection rotating motor 618, and is used to abut against the shaft extension rod 611. The far-rod stop block 617 is located on the side of the top sheet turnover rod 613 away from the shaft of the detection rotating motor 618, and is used to abut against the top sheet turnover rod 613. In this embodiment, the near-rod stop block 615 corresponds to the shaft extension rod 611, and the far-rod stop block 617 corresponds to the top sheet turnover rod 613. The near-rod stop block 615 serves as a turnover angle limiting component of the shaft extension rod 611, and the far-rod stop block 617 serves as a turnover angle limiting component of the top sheet turnover rod 613. When the liquid detection top sheet 616 is turned open, the near-rod stop block 615 limits the turnover angle of the shaft extension rod 611 to a specified angle, and at the same time, the far-rod stop block 617 also limits the turnover angle of the top sheet turnover rod 613 to the same specified angle. Specifically, the near-rod stop block 615 and the far-rod stop block 617 are arranged in parallel with each other, so that the opening angle of the liquid detection top sheet 616 is stable, and the damage caused by excessive turnover of the liquid detection top sheet 616 is avoided.

[0064] In one embodiment, see Figure 6The lens cleaning device 624 includes a cleaning fixed plate 6242, a feeding roller 6244, a winding roller 6246 and a cleaning pressing head 6248. The cleaning fixed plate 6242 is arranged on the lens cleaning frame 622. The feeding roller 6244, the winding roller 6246 and the cleaning pressing head 6248 are arranged on the cleaning fixed plate 6242. The feeding roller 6244 is used for unwinding the liquid absorbing belt. The winding roller 6246 is used for winding the liquid absorbing belt. The cleaning pressing head 6248 is located between the feeding roller 6244 and the winding roller 6246 and is used for pressing the liquid absorbing belt on the liquid detection substrate 614. In the embodiment, the cleaning fixed plate 6242 is used as a mounting and fixing component of the feeding roller 6244, the winding roller 6246 and the cleaning pressing head 6248. Specifically, the feeding roller 6244, the winding roller 6246 and the cleaning pressing head 6248 are located on one side of the cleaning fixed plate 6242 close to the liquid detection base 612. The feeding roller 6244 and the winding roller 6246 are used in cooperation. The feeding roller 6244 unwinds the liquid absorbing belt, and the winding roller 6246 synchronously winds the liquid absorbing belt back. The cleaning pressing head 6248 presses the liquid absorbing belt between the feeding roller 6244 and the winding roller 6246 on the liquid detection substrate 614, so as to wipe the residual liquid on the liquid detection substrate 614 when the liquid absorbing belt is wound.

[0065] Further, referring to Figure 7 The DNA liquid concentration test assembly 600 further includes a cleaning positioner 630. The cleaning positioner 630 includes a cleaning positioner motor 632, a cleaning positioner driving plate 634 and a cleaning positioner moving rail 636. The cleaning positioner motor 632 is arranged on the lens cleaning frame 622. The cleaning positioner driving plate 634 is connected with the extension shaft of the cleaning positioner motor 632 and the cleaning fixed plate 6242 respectively. The cleaning positioner moving rail 636 is arranged on the cleaning positioner driving plate 634, and the cleaning positioner motor 632 slides on the cleaning positioner moving rail 636. In the embodiment, the cleaning positioner motor 632 is used as a positioner mechanism of the cleaning pressing head 6248. The cleaning positioner motor 632 pushes the cleaning positioner driving plate 634. The cleaning positioner motor 632 moves the cleaning fixed plate 6242 through the cleaning positioner driving plate 634, so that the cleaning pressing head 6248 on the cleaning fixed plate 6242 is close to or away from the liquid detection substrate 614. The cleaning positioner moving rail 636 is used as a component for the cleaning positioner driving plate 634 to slide on the cleaning positioner motor 632, so as to keep smooth when the cleaning positioner driving plate 634 moves.

[0066] In another embodiment, referring toFigure 8 The DNA tube liquid concentration test assembly 600 further comprises a clear liquid swing member 640, which comprises a clear liquid swing motor 642, a clear liquid swing tooth rod 644 and a clear liquid swing gear 646. The clear liquid swing motor 642 is fixed on the clear liquid fixing plate 6242, the telescopic shaft of the clear liquid swing motor 642 is connected with the clear liquid swing tooth rod 644, the clear liquid swing tooth rod 644 is engaged with the clear liquid swing gear 646, the clear liquid swing gear 646 is rotationally arranged on the clear liquid fixing plate 6242, and the central shaft of the clear liquid swing gear 646 is connected with the clear liquid extrusion head 6248. In the embodiment, the clear liquid swing motor 642 serves as the power source of the clear liquid swing tooth rod 644. The clear liquid swing motor 642 is mounted on the clear liquid fixing plate 6242, the clear liquid swing tooth rod 644 and the clear liquid swing gear 646 are in meshing transmission connection with each other, the clear liquid swing motor 642 drives the clear liquid swing tooth rod 644 to extend and retract, the clear liquid swing tooth rod 644 drives the clear liquid swing gear 646 to rotate, the clear liquid extrusion head 6248 is connected with the central shaft of the clear liquid swing gear 646, so that the clear liquid swing gear 646 drives the clear liquid extrusion head 6248 to swing, thereby enabling the clear liquid extrusion head 6248 to extrude the liquid absorption belt on the liquid detection substrate 614, and the swing of the clear liquid extrusion head 6248 enables the liquid absorption belt to wipe the residual tube liquid on the liquid detection substrate 614.

[0067] Further, please refer to Figure 6The DNA tube liquid concentration test assembly 600 further comprises a clear liquid strip positioning member 650, which comprises a strip positioning movable connecting plate 652, a first telescopic cylinder 654, a second telescopic cylinder 656, and a plurality of strip conveying rods 658. The strip positioning movable connecting plate 652 is connected with the central shaft of the clear liquid swing gear 646. The first telescopic cylinder 654 is arranged on the side of the strip positioning movable connecting plate 652 away from the clear liquid swing gear 646. The longitudinal moving end of the first telescopic cylinder 654 is connected with the second telescopic cylinder 656. The transverse moving end of the second telescopic cylinder 656 is connected with the clear liquid extrusion head 6248. The plurality of strip conveying rods 658 are arranged around the edge of the strip positioning movable connecting plate 652. The strip conveying rods 658 are used to slide against the liquid absorbing strip to drive the liquid absorbing strip to pass through the end of the clear liquid extrusion head 6248. In this embodiment, the strip positioning movable connecting plate 652 serves as an intermediate plate between the clear liquid swing gear 646 and the first telescopic cylinder 654. The rotation of the clear liquid swing gear 646 is transmitted to the first telescopic cylinder 654 through the strip positioning movable connecting plate 652, thereby facilitating the swing operation of the clear liquid extrusion head 6248. The first telescopic cylinder 654 provides power for the longitudinal movement of the clear liquid extrusion head 6248. The second telescopic cylinder 656 provides power for the longitudinal movement of the clear liquid extrusion head 6248, so that the clear liquid extrusion head 6248 can move in multiple directions, facilitating the adjustment of the relative position between the clear liquid extrusion head 6248 and the liquid detection substrate 614, so that the clear liquid extrusion head 6248 accurately locates the liquid detection substrate 614 during wiping. The plurality of strip conveying rods 658 collectively drive the liquid absorbing strip to assist the transmission of the liquid absorbing strip, facilitating the smooth transmission of the liquid absorbing strip to the end of the clear liquid extrusion head 6248.

[0068] In another embodiment, the clear liquid extrusion head 6248 is an elastic wiping head. Specifically, a spring is arranged in the clear liquid extrusion head 6248 as a buffer to reduce the contact between the liquid absorbing strip and the end of the clear liquid extrusion head 6248.

[0069] In another embodiment, referring to Figure 6 The clear liquid strip positioning member 650 further comprises at least one strip blocking ring 651, which is sleeved on the strip conveying rod 658 and is used to slide against the side of the liquid absorbing strip away from the strip positioning movable connecting plate 652. In this embodiment, the strip blocking ring 651 is sleeved with the strip conveying rod 658. The strip blocking ring 651 serves as a limiting component for the liquid absorbing strip during transmission, so as to limit the liquid absorbing strip on the strip conveying rod 658 and prevent the liquid absorbing strip from detaching from the strip conveying rod 658 during transmission.

[0070] In another embodiment, referring to Figure 7 The clear liquid positioner 630 further comprises a positioner sliding block 638, and the clear liquid positioner driving plate 634 has a "U" shaped structure, and the clear liquid positioner driving plate 634 comprises a first positioner part 6342 and a second positioner part 6344 which are connected perpendicularly to each other, the first positioner part 6342 is connected with the telescopic shaft of the clear liquid positioner motor 632, and the first positioner part 6342 is located on the side of the clear liquid positioner motor 632 which is away from the clear liquid fixed plate 6242, the second positioner part 6344 is further connected with the clear liquid fixed plate 6242, the clear liquid positioner moving rail 636 is located on the side of the second positioner part 6344 which is close to the clear liquid positioner motor 632, and the positioner sliding block 638 is connected with the clear liquid positioner motor 632 and is slidingly arranged on the clear liquid positioner moving rail 636. In this embodiment, the first positioner part 6342 and the second positioner part 6344 form a "U" shaped structure, specifically, two ends of one first positioner part 6342 are connected with one second positioner part 6344 respectively, and the two second positioner parts 6344 are parallel to the telescopic shaft of the clear liquid positioner motor 632. The positioner sliding block 638 is slidingly connected with the clear liquid positioner moving rail 636 which is located on the second positioner part 6344, so that when the second positioner part 6344 drives the clear liquid fixed plate 6242 to extend and retract, the second positioner part 6344 moves more stably relative to the clear liquid positioner motor 632, for example, the clear liquid positioner moving rail 636 is parallel to the telescopic shaft of the clear liquid positioner motor 632. Moreover, the "U" shaped structure formed by the first positioner part 6342 and the second positioner part 6344 reduces the distance between the clear liquid positioner motor 632 and the clear liquid fixed plate 6242, thereby reducing the space occupation volume of the clear liquid positioner 630.

[0071] In another embodiment, referring to Figure 9The DNA tube liquid ultra-micro testing device 10B further comprises a DNA tube cover lifting assembly 700, which comprises a DNA tube installation base 710, a cover lifting motor 720 and a cover lifting rotating rod 730. The DNA tube installation base 710 is arranged adjacent to the DNA tube placing seat 500 and is used for placing the capped test tube transferred from the DNA tube placing seat 500. The cover lifting motor 720 is connected with the DNA tube installation base 710. The rotating shaft of the cover lifting motor 720 is connected with the cover lifting rotating rod 730. The cover lifting rotating rod 730 abuts against the inner side of the cap of the test tube. In this embodiment, the DNA tube installation base 710 serves as a placing area of the capped test tube transferred from the DNA tube placing seat 500. For example, the liquid ultra-micro testing device further comprises a test tube transferring assembly, which is used for transferring the capped test tube from the DNA tube placing seat 500 to the DNA tube installation base 710. At this time, the cap of the test tube on the DNA tube installation base 710 is in a closed state. The cover lifting motor 720 is fixed on the DNA tube installation base 710. The cover lifting motor 720 serves as a rotating power source of the cover lifting rotating rod 730. The rotating shaft of the cover lifting motor 720 drives the cover lifting rotating rod 730 to rotate, so that the cover lifting rotating rod 730 lifts the cap of the test tube to realize uncapping of the test tube, thereby facilitating extraction of the liquid in the test tube.

[0072] In another embodiment, referring to Figure 9 The DNA tube installation base 710 is provided with a test tube installation groove 702, which accommodates part of the test tube. In this embodiment, the test tube installation groove 702 is arranged on the DNA tube installation base 710. The test tube installation groove 702 accommodates part of the test tube, i.e., the test tube installation groove 702 serves as a placing position of the test tube, so that the test tube is stably placed on the DNA tube installation base 710. Specifically, at least part of the body of the test tube is arranged in the test tube installation groove 702, and the cap of the test tube is arranged outside the test tube installation groove 702.

[0073] In another embodiment, referring to Figure 9The cover lifting rotating rod 730 comprises a first cover lifting rotating part 732 and a second cover lifting rotating part 734 which are connected perpendicularly to each other. The first cover lifting rotating part 732 is further connected with the rotating shaft of the cover lifting motor 720. The second cover lifting rotating part 734 is arranged in parallel with the rotating shaft of the cover lifting motor 720. The second cover lifting rotating part 734 is used to abut against the inner side of the cover of the to-be-tested tube when the cover is opened, so as to open the cover of the to-be-tested tube. In the embodiment, the first cover lifting rotating part 732 is an extension of the rotating shaft of the cover lifting motor 720. The rotation of the first cover lifting rotating part 732 is transmitted to the second cover lifting rotating part 734. The center of the overturning of the second cover lifting rotating part 734 is shifted from the rotating shaft of the cover lifting motor 720 to the connection position of the first cover lifting rotating part 732. Thus, the overturning swing arm of the second cover lifting rotating part 734 is increased, which facilitates the second cover lifting rotating part 734 to quickly open the cover of the to-be-tested tube. Moreover, when the cover is opened, the second cover lifting rotating part 734 always abuts against the inner side of the cover of the to-be-tested tube. The maximum distance between the second cover lifting rotating part 734 and the DNA tube mounting base 710 is less than or equal to the maximum distance between the cover of the to-be-tested tube and the DNA tube mounting base 710.

[0074] In another embodiment, referring to Figure 9 The DNA tube liquid ultramicro testing device 10B further comprises a DNA tube cover clamping assembly 800. The DNA tube cover clamping assembly 800 comprises a cover clamping telescopic motor 810 and a cover clamping translation plate 820. The cover clamping telescopic motor 810 is arranged on the DNA tube mounting base 710. The cover clamping translation plate 820 is connected with the telescopic shaft of the cover clamping telescopic motor 810. The cover clamping translation plate 820 is used to abut against the outer side of the cover of the to-be-tested tube when the cover is closed, so as to close the cover of the to-be-tested tube. In the embodiment, the cover clamping telescopic motor 810 is fixed on the DNA tube mounting base 710. Specifically, the cover clamping telescopic motor 810 is fixed on the end of the DNA tube mounting base 710 which is close to the outer side of the cover of the to-be-tested tube. The cover clamping translation plate 820 is located between the cover clamping telescopic motor 810 and the to-be-tested tube. In this way, the cover clamping telescopic motor 810 pushes the cover clamping translation plate 820 to move towards the outer side of the cover of the to-be-tested tube, so that the cover clamping translation plate 820 abuts against the outer side of the cover of the to-be-tested tube, thereby facilitating the cover clamping translation plate 820 to close the cover of the to-be-tested tube. Moreover, when the cover is closed, the cover clamping translation plate 820 always abuts against the outer side of the cover of the to-be-tested tube. The distance between the bottom surface of the cover clamping translation plate 820 and the DNA tube mounting base 710 is greater than or equal to the distance between the outer side of the cover of the to-be-tested tube and the DNA tube mounting base 710 when the cover is closed.

[0075] In one of the embodiments, the present disclosure further provides a DNA tube liquid concentration adjustment system, comprising the DNA tube liquid concentration adjustment device of any one of the above embodiments. In this embodiment, the DNA tube liquid concentration adjustment device comprises a DNA tube liquid shaking device, a DNA tube liquid ultra-micro testing device and a DNA tube liquid concentration adjustment device; the DNA tube liquid shaking device is used for shaking the liquid in the DNA tubes; the DNA tube liquid ultra-micro testing device is used for detecting the concentration of the liquid in the DNA tubes; the DNA tube liquid concentration adjustment device comprises a DNA tube liquid adjustment mechanism and a DNA tube liquid dilution cup, the DNA tube liquid dilution cup is used for containing dilution liquid, the DNA tube liquid adjustment mechanism comprises a tube liquid adjustment base, a tube liquid adjustment mechanical arm and a tube liquid extraction needle, the tube liquid adjustment mechanical arm is arranged on the tube liquid adjustment base, the tube liquid extraction needle is arranged on the tube liquid adjustment mechanical arm, and the tube liquid extraction needle is used for extracting the liquid in the DNA tubes to the testing position of the DNA tube liquid ultra-micro testing device to test the concentration of the liquid in the DNA tubes; the liquid extraction control end of the tube liquid extraction needle is connected with the tube liquid concentration adjustment end of the DNA tube liquid ultra-micro testing device, and the tube liquid extraction needle is also used for extracting the dilution liquid in the DNA tube liquid dilution cup to the DNA tubes when the concentration of the liquid in the DNA tubes is greater than the preset concentration, so that the concentrations of the liquids in the DNA tubes are equal. After the concentration test of the DNA tube liquid, when the concentration of the DNA tube liquid is too high, the tube liquid adjustment mechanical arm drives the tube liquid extraction needle to the DNA tube liquid dilution cup, the dilution liquid in the DNA tube liquid dilution cup is extracted into the DNA tubes, and then the above test and dilution operation are repeated until the concentration of the liquid in the DNA tubes reaches the predetermined concentration, so that the concentrations of the liquids in the DNA tubes remain consistent.

[0076] The above embodiments only express several embodiments of the present disclosure, and the description is more specific and detailed, but it cannot be understood as the limitation of the scope of the patent. It should be noted that for ordinary skilled in the art, without departing from the concept of the present disclosure, several modifications and improvements can be made, which are within the scope of the present disclosure. Therefore, the protection scope of the present disclosure patent should be subject to the appended claims.

Claims

1. A DNA test tube liquid concentration adjustment device, characterized in that: include: A DNA test tube liquid shaking device, which is used to shake the liquid in multiple DNA test tubes; DNA tube liquid ultra-micro testing device, the DNA tube liquid ultra-micro testing device is used to detect the concentration of the liquid in the DNA test tube; A DNA tube liquid concentration adjustment device, the DNA tube liquid concentration adjustment device comprising a DNA tube liquid adjustment mechanism and a DNA tube liquid dilution cup, the DNA tube liquid dilution cup being used to hold a diluent, the DNA tube liquid adjustment mechanism comprising a tube liquid adjustment base, a tube liquid adjustment mechanical arm, and a tube liquid extraction needle, the tube liquid adjustment mechanical arm being disposed on the tube liquid adjustment base, the tube liquid extraction needle being disposed on the tube liquid adjustment mechanical arm, the tube liquid extraction needle being used to extract liquid from a DNA test tube to a test position of the DNA tube liquid ultra-micro testing device to test the concentration of the liquid in the DNA test tube; the liquid extraction control end of the tube liquid extraction needle being connected to the tube liquid concentration adjustment end of the DNA tube liquid ultra-micro testing device, the tube liquid extraction needle being further used to extract the diluent from the DNA tube liquid dilution cup to a DNA test tube when the concentration of the liquid in the DNA test tube is greater than a preset concentration, so as to equalize the concentrations of the liquids in each DNA test tube; Wherein, the DNA tube liquid ultra-trace testing device includes a DNA test tube placement seat and a DNA tube liquid concentration testing assembly, the DNA test tube placement seat is used to place DNA test tubes in batches; the DNA tube liquid concentration testing assembly includes a tube liquid concentration detection component and a lens liquid detection component, the tube liquid concentration detection component includes a liquid detection base, a liquid detection substrate, a liquid detection top plate and a detection rotating motor, the liquid detection substrate is arranged on the liquid detection base, the liquid detection top plate is rotatably connected to the liquid detection substrate, the detection rotating motor is connected to the liquid detection base, and the rotating shaft of the detection rotating motor is connected to the liquid detection top plate, so that the liquid detection top plate is flipped over to fit the liquid detection substrate during tube liquid testing; the lens liquid detection component includes a lens liquid cleaning rack and a lens liquid cleaner, the lens liquid cleaning rack is arranged adjacent to the liquid detection base, the lens liquid cleaner is slidably arranged on the lens liquid cleaning rack, and the wiping end of the lens liquid cleaner is used to swing and wipe the liquid detection substrate; The lens liquid cleaner includes a liquid cleaning fixed plate, a tape feed wheel, a tape take-up wheel and a liquid cleaning extrusion head, the liquid cleaning fixed plate is arranged on the lens liquid cleaning rack, the tape feed wheel, the tape take-up wheel and the liquid cleaning extrusion head are all arranged on the liquid cleaning fixed plate, the tape feed wheel is used to unwind the liquid suction tape, the tape take-up wheel is used to rewind the liquid suction tape, the liquid cleaning extrusion head is located between the tape feed wheel and the tape take-up wheel, and the liquid cleaning extrusion head is used to squeeze the liquid suction tape onto the liquid detection substrate; the DNA tube liquid concentration test assembly also includes a liquid cleaning position adjustment part and a liquid cleaning swing part, the liquid cleaning position adjustment part includes a liquid cleaning position adjustment motor, a liquid cleaning position adjustment drive plate and a liquid cleaning position adjustment movable rail, the liquid cleaning position adjustment motor is arranged On the lens clear liquid rack, the clear liquid positioning drive plate is respectively connected to the telescopic shaft of the clear liquid positioning motor and the clear liquid fixed plate, the clear liquid positioning movable rail is arranged on the clear liquid positioning drive plate, and the clear liquid positioning motor slides on the clear liquid positioning movable rail; the clear liquid swinging part includes a clear liquid swinging motor, a clear liquid swinging gear rod and a clear liquid swinging gear, the clear liquid swinging motor is fixed on the clear liquid fixed plate, the telescopic shaft of the clear liquid swinging motor is connected to the clear liquid swinging gear rod, the clear liquid swinging gear rod is meshed with the clear liquid swinging gear, the clear liquid swinging gear is rotatably arranged on the clear liquid fixed plate, and the central axis of the clear liquid swinging gear is connected to the clear liquid extrusion head.

2. The DNA test tube liquid concentration adjustment device according to claim 1, characterized in that: The DNA tube liquid adjustment mechanism also includes a liquid collection slide rail, which is installed on the tube liquid adjustment mechanical arm, and the tube liquid extraction needle is slidably connected to the liquid collection slide rail.

3. The DNA test tube liquid concentration adjustment device according to claim 2, characterized in that: The DNA tube liquid adjustment mechanism further comprises a sampling slider, which is slidably arranged on the liquid collection slide rail, and the tube liquid extraction needle is fixed on the sampling slider.

4. The DNA test tube liquid concentration adjustment device according to claim 1, characterized in that: The DNA tube liquid adjustment mechanism further includes a liquid level probe, which is arranged on the tube liquid adjustment mechanical arm and is used to detect the liquid level of the liquid in the DNA test tube.

5. The DNA test tube liquid concentration adjustment device according to claim 1, characterized in that: The DNA tube liquid adjustment mechanism also includes an ultrasonic liquid level detector, which is installed on the tube liquid adjustment mechanical arm and is used to recheck the liquid level of the liquid in the DNA test tube.

6. The DNA test tube liquid concentration adjustment device according to claim 1, characterized in that: The DNA tube liquid adjustment mechanism also includes a test tube grabbing cylinder, which is fixedly connected to the tube liquid adjustment mechanical arm and is used to grab the DNA test tube.

7. The DNA test tube liquid concentration adjustment device according to claim 1, characterized in that: The DNA test tube liquid shaking device includes a DNA liquid shaking component and a DNA test tube locking component, the DNA liquid shaking component includes an oscillating polarization motor and a polarization plate, the rotating shaft of the oscillating polarization motor is connected to the polarization plate to make the polarization plate rotate eccentrically; the DNA test tube locking component includes a DNA test tube locking seat and a DNA test tube locking piece, the DNA test tube locking seat is connected to the side of the polarization plate away from the oscillating polarization motor, the DNA test tube locking seat has at least one test tube installation area, the test tube installation area is used to accommodate a variety of test tube assembly fixtures, and the test tube assembly fixture is used to hold DNA liquid; the DNA The test tube locking piece is arranged on the DNA test tube locking seat, and the DNA test tube locking piece is also used to connect with the test tube assembly fixture to install the test tube assembly fixture on the DNA test tube locking seat; wherein, the DNA liquid oscillation component also includes an oscillation positioning plate and a laser sensor, the oscillation positioning plate is mounted on the rotating shaft of the oscillation polarization motor, the oscillation positioning plate is provided with a positioning window, the laser sensor is arranged adjacent to the oscillation positioning plate, and the output end of the laser sensor is used to connect with the central control end of the oscillation polarization motor, so that when the oscillation polarization motor is started or reset, the sensing end of the laser sensor is arranged opposite to the positioning window.

8. A DNA test tube liquid calibration system, characterized in that: It comprises the DNA test tube liquid concentration adjustment device as described in any one of claims 1 to 7.

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