A closed flow cytometry sample processing device

Abstract

The present invention relates to the field of cell sample storage technology, and in particular to a closed flow cell sample processing device. The device comprises a sealing ring membrane that separates the storage cavity into a first chamber and a second chamber that are not connected to each other, and the sealing ring membrane comprises a sealing body, a first opening blind seam, a second opening blind seam and a guide slot, wherein the second opening blind seam corresponds to the position of the first opening blind seam; the guide slot is used to quickly break the sealing ring membrane and guide the cell sample to be extruded vertically in a jet-like shape after being pressurized. The present invention can break the sealing ring membrane by pressure in a completely closed state, and extrudes the cell sample in a jet-like shape from the discharge head of the storage device in a jet-like shape after being pressurized, and vertically injects it into the sample tube to be mixed. During the entire cell sample adding operation, the cell sample does not come into contact with the external environment, thereby ensuring that the cell sample is not contaminated by the external environment to a great extent, avoiding external contamination, and improving the sterility of the sample processing.

Description

Technical Field

[0001] The present invention relates to the technical field of cell sample storage, and in particular to a closed flow cell sample processing device. Background Art

[0002] Flow cytometry is a technique that uses fluorescent signals from single cells or other biological particles in suspension to achieve high-speed, individual cell quantitative analysis and sorting. The main steps include: 1. Preparation of single-cell suspension; 2. Antibody selection and multi-color combination design; 3. Statistical results; 4. Screening of various cell types. During the antibody selection step, multiple different antibodies need to be added to the sample sequentially or simultaneously to adapt to the various markers that need to be detected in the sample.

[0003] During the current antibody addition process, storage boxes of multiple antibodies need to be opened one by one, and a pipette or straw is used to draw out the antibodies one by one and add them to the sample tube to mix with the sample. During this process, the antibodies will be exposed to the external environment for a long time, increasing the risk of environmental pollution. At the same time, when the current antibody storage box is drawn to the end, a layer of cell samples remaining at the bottom of the box is not easily sucked out by a pipette or straw, and is generally disposed of directly by discarding it. When faced with high-cost and difficult-to-obtain cell samples, the economic cost is high. Summary of the Invention

[0004] The present invention aims to at least improve one of the technical problems existing in the prior art. To this end, the present invention provides a closed flow cytometer sample processing device.

[0005] The technical solution of the present invention is as follows: A closed flow cytometer sample processing device, comprising:

[0006] A reservoir having a cavity and a discharge head, wherein the cavity is in communication with the discharge head;

[0007] A sealing ring membrane is used to separate the cavity into a first chamber and a second chamber that are not connected to each other. The sealing ring membrane includes:

[0008] a sealing body connected to the reservoir;

[0009] a plurality of first open blind slits, formed on the surface of the sealing body near the first chamber;

[0010] A plurality of second open blind slits are provided on the other surface of the sealing body, corresponding to the position of each first open blind slit;

[0011] The guide notch is formed on the sealing body away from the inner wall of the reservoir, and is used to quickly break the sealing ring membrane and guide the cell sample to be squeezed out of the discharge head in a vertical jet shape instantly after being pressurized.

[0012] In a possible technical solution, the sealing body further has a surface that gradually sinks toward the center, so that the overall thickness of the sealing body gradually decreases from the circumference to the center, and the thickness reaches a minimum value at the center. Through the above arrangement, the center position of the sealing ring membrane is ensured to be the weakest point. Therefore, when it is subjected to pressure from top to bottom, it will first break along the first opening blind seam at the center, thereby ensuring the smooth discharge of the cell sample.

[0013] In a possible technical solution, further, the cross-sections of the first opening blind seam and the second opening blind seam are both triangular wedge-shaped, and the depth of the first opening blind seam is greater than the depth of the second opening blind seam, so that the sealing ring film is subjected to forces in other directions, and the pressure threshold of the sealing ring film is higher, so that it is not easy to be torn, further improving the safety of the operator after accidental touch.

[0014] In a possible technical solution, further, the guide notch and the first opening blind slit are located on the same side, and the angle between the guide notch and the first opening blind slit is in the range of 30° to 90°, so that the position of a guide notch is exactly in the middle position between the two first opening blind slits. Therefore, when the guide notch provides a breaking position, the pressure of the two first opening blind slits on both sides can be synchronously transmitted, further improving the uniform force at various locations of the sealing ring membrane during the tearing process, thereby ensuring that the tearing speed at various locations remains uniform, and further ensuring the uniformity of the spraying state when the cell sample is discharged.

[0015] In a possible technical solution, further, the plurality of first open blind seams are symmetrically distributed about the center of the sealing body, so that the pressure is more uniform.

[0016] In a possible technical solution, further, the sealing body near the center has a straight portion, a circular portion and an arc-shaped portion, and the straight portion, the circular portion and the arc-shaped portion are connected in sequence to form a guide slot, so that the guide slot can guide the pressure into the interior thereof and squeeze toward both sides of the sealing ring membrane in an inclined posture, thereby just keeping synchronization with the tearing direction of the first opening blind seam and the second opening blind seam toward both ends, thereby increasing the speed at which the sealing ring membrane is torn, and at the same time increasing its reaction speed when it is broken under pressure, and the guide slot itself will open outward as the first opening blind seam is torn, so that the residual antibody inside it can be effectively squeezed out when the reservoir is subsequently squeezed, further improving the practical effect of the device.

[0017] In a possible technical solution, further, the straight portion is located on an extension line of a surface trajectory of the sealing body that is symmetrical thereto.

[0018] In a possible technical solution, further, the first opening blind slits are distributed in a cross shape, so as to ensure that the sealing ring membrane can be subjected to sufficient pressure to guide the cell sample to be ejected instantly after being pressurized.

[0019] In a possible technical solution, further, the end of the reservoir away from the discharge head is provided with a sealing edge, and the discharge head has an outlet that can be sealed by a sealing plug, wherein the end of the discharge head is detachably provided with a sealing plug.

[0020] In a possible technical solution, further comprising:

[0021] Metal damping wires are installed on both sides of the reservoir. When the operator processes the cell sample remaining in the reservoir, the operator can pinch the tail of the reservoir and curl the reservoir downward from the edge sealing position in sequence. At the same time, the metal damping wire is deformed during curling to effectively curl the reservoir in sequence into a tightly rolled state, so that the residual antibodies inside the reservoir can be fully discharged from the discharge head, further improving its practicality.

[0022] The sealing ring membrane is supported by medical-grade sterile elastic material as a whole, and the surface finish of the sealing ring membrane is RA0.2-RA0.3. Through the above setting, it can be ensured that when the first opening blind seam and the second opening blind seam are torn under pressure, the overall incision is smooth and has no burrs, which facilitates the smooth passage of antibodies.

[0023] According to the closed flow cytometer sample processing device of the present invention, by providing a reservoir, a sealing ring membrane, an open blind seam and a guide slot, the sealing ring membrane can be broken by pressure in a completely closed state, and the cell sample is squeezed out of the discharge head of the reservoir in a jet state after being pressurized, and vertically injected into the sample tube to be mixed. During the entire cell sample addition operation, the cell sample does not come into contact with the external environment, thereby greatly ensuring that the cell sample is not contaminated by the external environment, avoiding external contamination, and improving the sterility of the sample processing. At the same time, the operator can use the operation method of squeezing and curling the reservoir to fully discharge the cell sample for use, thereby maximizing the utilization rate of the cell sample and increasing the economic efficiency of the sample processing.

[0024] Additional aspects and advantages of the present invention will be set forth in part in the description which follows and, in part, will be obvious from the description which follows, or may be learned by practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the following briefly introduces the drawings required for use in the embodiments. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0026] Figure 1 It is a schematic diagram of the overall structure of the present invention;

[0027] Figure 2 It is a schematic diagram of the side three-dimensional structure of the present invention;

[0028] Figure 3 It is a schematic diagram of the overall cross-sectional structure of the present invention;

[0029] Figure 4 For the present invention Figure 3 Schematic diagram of the cross-sectional structure at AA;

[0030] Figure 5 Schematic diagram of the sealing ring membrane structure of the present invention;

[0031] Figure 6 Schematic diagram of the side cross-section structure of the sealing ring membrane of the present invention;

[0032] Figure 7 For the present invention Figure 6 A schematic diagram of the enlarged structure at point A;

[0033] Figure 8 A top view of the sealing ring membrane of the present invention;

[0034] Figure 9 For the present invention Figure 8 Schematic diagram of the cross-sectional structure at BB;

[0035] Figure 10 Schematic diagram of a partial cross-sectional structure of the sealing ring membrane of the present invention;

[0036] Figure 11 A schematic diagram of the direction of pressure guidance during the compression process of the sealing ring membrane of the present invention;

[0037] Figure 12 This is a schematic diagram of the storage extrusion and release process of the present invention;

[0038] Figure 13 The stress cloud diagram and the corresponding stress direction diagram of the internal stress of the sealing ring membrane of the present invention when it is under pressure;

[0039] Figure 14 The pressure cloud diagram of the sealing ring membrane of the present invention when it is under pressure;

[0040] Figure 15This is a line diagram of the contact pressure applied to the upper boundary of the sealing ring membrane of the present invention.

[0041] Reference numerals:

[0042] Storage container 1, chamber 10, discharge head 11, first chamber 101, second chamber 102, edge sealing 12

[0043] Sealing ring membrane 2; sealing body 20, straight portion 201, circular portion 202, arc portion 203, first opening blind seam 21, second opening blind seam 22, guide notch 23;

[0044] Sealing plug 4, metal damping wire 5. DETAILED DESCRIPTION

[0045] The embodiments of the present invention are described in detail below. The embodiments described with reference to the accompanying drawings are exemplary. It should be understood that the specific embodiments described herein are only used to explain the present application and are not used to limit the present application.

[0046] It should be noted that when an element is referred to as being “fixed to” another element, it may be directly on the other element or there may be an intermediate element. When an element is referred to as being “connected to” another element, it may be directly connected to the other element or there may be an intermediate element.

[0047] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by those skilled in the art to which this invention pertains. The terms used in this specification of the present invention are for the purpose of describing specific embodiments only and are not intended to limit the present invention. The term "and / or" as used herein includes any and all combinations of one or more of the associated listed items.

[0048] In the specification, claims, and accompanying drawings of this application, the terms "first," "second," "third," and the like are used to distinguish different objects and are not used to describe a particular order. Furthermore, the terms "including," "comprising," "having," and any variations thereof are intended to cover non-exclusive inclusions. For example, a list of steps or elements may be included, or alternatively, steps or elements not listed may be included, or other steps or elements may be included that are inherent to the process, method, product, or apparatus.

[0049] Only portions relevant to the present application are shown in the accompanying drawings, not all of them. Before discussing the exemplary embodiments in more detail, it should be noted that some exemplary embodiments are described as processes or methods depicted as flow charts. Although the flow charts describe the various operations (or steps) as being processed sequentially, many of the operations can be performed in parallel, concurrently, or simultaneously. In addition, the order of the various operations can be rearranged. The process can be terminated when its operations are completed, but can also have additional steps not included in the accompanying drawings. The process can correspond to a method, function, procedure, subroutine, subprogram, etc.

[0050] Example 1

[0051] like Figures 1 to 15 As shown, this embodiment provides a closed flow cytometer sample processing device, which includes:

[0052] The reservoir 1 has a chamber 10 and a discharge head 11, wherein the chamber 10 is in communication with the discharge head 11;

[0053] The sealing ring membrane 2 divides the cavity 10 into a first chamber 101 and a second chamber 102 which are not connected to each other. The sealing ring membrane 2 includes:

[0054] A sealing body 20 connected to the reservoir 1;

[0055] A plurality of first open blind slits 21 are formed on the surface of the sealing body 20 near the first chamber 101 and are not connected to the second chamber 102;

[0056] A plurality of second blind opening slits 22 are formed on the other surface of the sealing body 20 and correspond to the positions of each first blind opening slit 21;

[0057] The guide notch 23 is formed on the sealing body 20 away from the inner wall of the reservoir 1 , and is used to quickly break the sealing membrane 2 and guide the cell sample to be squeezed out of the discharge head 11 in a vertical jet shape instantly after being pressurized.

[0058] It should be noted that, in this embodiment, the sealing body 20 has a surface that gradually sinks toward the center, so that the overall thickness of the sealing body 20 gradually decreases from the circumference to the center, and the thickness reaches a minimum value at the center. Through the above arrangement, the center position of the sealing ring membrane 2 is ensured to be the weakest point. Therefore, when it is subjected to pressure from top to bottom, it will first break along the first opening blind seam 21 at the center, thereby ensuring the smooth discharge of the cell sample.

[0059] in, Figure 13-14 The horizontal and vertical coordinates are both size coordinates.

[0060] It should be noted that, in this embodiment, the cross-sections of the first opening blind seam 21 and the second opening blind seam 22 are both triangular wedge-shaped, and the depth of the first opening blind seam 21 is greater than the depth of the second opening blind seam 22, so that the sealing ring film 2 is subjected to forces in other directions, such as forces in the opposite direction from bottom to top. The pressure threshold of the sealing ring film 2 is relatively high, so it is not easy to be torn, further improving the safety of the operator after accidental touch.

[0061] Among them, Figure 13 As shown in FIG, the stress cloud diagram of the internal stress of the sealing ring film 2 and the corresponding stress direction thereof when the sealing ring film 2 is under pressure are shown. Figure 14 As shown, the distribution of the pressure at the center position and inside the guide groove 23 of the sealing ring membrane 2 as a whole is shown. Figure 15 As shown, the distribution of contact pressure of the upper boundary of the sealing ring membrane 2 after being subjected to pressure is shown. It can be seen that as the squeezing continues, the stress generated by the sealing ring membrane 2 and the pressure it is subjected to continue to increase.

[0062] It should be noted that, in this embodiment, the guide notch 23 and the first opening blind slit 21 are located on the same side, and the guide notch 23 and the first opening blind slit 21 are distributed at an angle of 30° to 90°, so that the position of one guide notch 23 is exactly in the middle position between the two first opening blind slits 21. Therefore, when the guide notch 23 provides a breaking position, the pressure of the two first opening blind slits 21 on both sides can be simultaneously transmitted, further improving the uniform force at various locations of the sealing ring film 2 during the tearing process, thereby ensuring that the tearing speed at various locations remains uniform, and further ensuring the uniformity of the spray state when the cell sample is discharged.

[0063] It should be noted that, in this embodiment, the plurality of first open blind seams 21 are symmetrically distributed about the center of the sealing body 20 , so that the pressure is more uniform.

[0064] It should be noted that, in this embodiment, the sealing body 20 near the center has a straight portion 201, a circular portion 202 and an arc-shaped portion 203, and the straight portion 201, the circular portion 202 and the arc-shaped portion 203 are connected in sequence to form a guide slot 23, so that the guide slot 23 can guide the pressure into the inside thereof and squeeze toward the two sides of the sealing ring film 2 in an inclined posture, thereby just keeping synchronization with the tearing direction of the first opening blind seam 21 and the second opening blind seam 22 toward both ends, thereby increasing the speed at which the sealing ring film 2 is torn, and at the same time increasing its reaction speed when it is broken under pressure, and the guide slot 23 itself will open outward as the first opening blind seam 21 is torn, so that the residual antibody inside it can be effectively squeezed out when the reservoir 1 is subsequently squeezed, further improving the practical effect of the device.

[0065] It should be noted that, in this embodiment, the guiding notch 23 is a convex structure as a whole, and the guiding notch 23 and the top surface of the sealing ring film 2 are distributed in an inclined manner.

[0066] The specific implementation method is as follows: by utilizing the above-mentioned setting, the guide slot 23 can guide a small portion of the antibody squeezed by the operator into the guide slot 23, and guide its pressure accordingly, thereby squeezing the weakest position in the center of the sealing ring membrane 2, causing it to reach the maximum pressure resistance value and break, and tearing along the direction of the first opening blind seam 21 and the second opening blind seam 22, thereby smoothly discharging the cell sample from the reservoir 1, fully improving the sensitivity of the sealing ring membrane 2 after being subjected to force.

[0067] It should be noted that, in this embodiment, the diameter of the sealing ring membrane 2 is equal to the width of the cavity 10, and the inner wall surface of the cavity 10 is coated with a layer of polymer material to ensure that when the reservoir 1 discharges the cell sample, the cell sample is pressurized and instantly squeezed out of the reservoir 1 in a jet state. At the same time, due to the alienation effect of the polymer material layer, the cell sample is not easily adhered to the inner wall of the cavity 10 during the discharge process.

[0068] It should be noted that, in this embodiment, the straight portion 201 is located on an extension line of a surface trajectory of the sealing body 20 that is symmetrical thereto.

[0069] It should be noted that, in this embodiment, the first blind slits 21 are distributed in a cross shape, so as to ensure that the sealing ring membrane 2 can be subjected to sufficient pressure to guide the cell sample to be ejected instantly after being pressurized.

[0070] It should be noted that, in this embodiment, a sealing edge 12 is provided at the end of the reservoir 1 away from the discharge head 11 , and the discharge head 11 has an outlet that can be sealed by a sealing plug 4 , wherein the end of the discharge head 11 is detachably fitted with a sealing plug 4 .

[0071] It should be noted that, in this embodiment, the sealing ring membrane 2 is supported by a medical-grade sterile elastic material as a whole, and the surface finish of the sealing ring membrane 2 is RA0.2 to RA0.3, which can ensure that when the first opening blind seam 21 and the second opening blind seam 22 are torn under pressure, the overall incision is smooth and has no burrs, facilitating the smooth passage of antibodies.

[0072] Example 2

[0073] like Figure 3 As shown, this embodiment makes further improvements on the basis of embodiment 1, and provides a closed flow cytometer sample processing device, which includes:

[0074] The reservoir 1 has a chamber 10 and a discharge head 11, wherein the chamber 10 is in communication with the discharge head 11;

[0075] The sealing ring membrane 2 divides the cavity 10 into a first chamber 101 and a second chamber 102 which are not connected to each other. The sealing ring membrane 2 includes:

[0076] A sealing body 20 connected to the reservoir 1;

[0077] A plurality of first open blind slits 21 are formed on the surface of the sealing body 20 near the first chamber 101 and are not connected to the second chamber 102;

[0078] A plurality of second blind opening slits 22 are formed on the other surface of the sealing body 20 and correspond to the positions of each first blind opening slit 21;

[0079] The guide notch 23 is formed on the sealing body 20 away from the inner wall of the reservoir 1 , and is used to quickly break the sealing membrane 2 and guide the cell sample to be squeezed out of the discharge head 11 in a vertical jet shape instantly after being pressurized.

[0080] The metal damping wire 5 is installed on both sides of the reservoir 1. When the operator processes the cell sample remaining in the reservoir 1, the operator can pinch the tail of the reservoir 1 and curl the reservoir downward from the edge seal 12. At the same time, the metal damping wire 5 is deformed during curling to effectively curl the reservoir 1 into a tightly rolled state, so that the residual antibodies inside the reservoir are fully discharged from the discharge head 11, further improving its practicality.

[0081] According to the closed flow cytometer sample processing device of the present invention, by providing a reservoir, a sealing ring membrane, an open blind seam and a guide slot, the sealing ring membrane can be broken by pressure in a completely closed state, and the cell sample is squeezed out of the discharge head of the reservoir in a jet state after being pressurized, and vertically injected into the sample tube to be mixed. During the entire cell sample addition operation, the cell sample does not come into contact with the external environment, thereby greatly ensuring that the cell sample is not contaminated by the external environment, avoiding external contamination, and improving the sterility of the sample processing. At the same time, the operator can use the operation method of squeezing and curling the reservoir to fully discharge the cell sample for use, thereby maximizing the utilization rate of the cell sample and increasing the economic efficiency of the sample processing.

[0082] This embodiment provides a method for processing antibody materials, which is as follows:

[0083] Prepare a sample processing device loaded with antibody material, remove the sealing plug 4, and simultaneously align the outlet position of the discharge head 11 of the reservoir 1 with the inlet of the sample tube. Specifically, the outlet position of the sample processing device can usually be set to be slightly smaller than the inlet size of a general sample tube to facilitate operation by the experimenter.

[0084] The operator pinches the top of the reservoir 1 with his index finger and thumb, and strokes the reservoir 1 from top to bottom. At this time, the antibody material inside it is compressed and squeezes the sealing ring film 2 at the lower outlet position downward. At this time, the guide groove 23 can guide a small part of the antibody squeezed by the operator into the guide groove 23, and squeeze it towards the two sides of the sealing ring film 2 in an inclined posture, so as to keep synchronization with the tearing direction of the first opening blind seam 21 and the second opening blind seam 22 towards both ends, and squeeze the weakest position at the center of the sealing ring film 2 to make it reach the maximum compressive strength and break, and tear cross-shaped along the direction of the first opening blind seam 21 and the second opening blind seam 22. At this time, the quickly broken sealing ring film 2 can guide the antibody After being pressurized, the body material is instantly squeezed out of the reservoir 1 in a jet state and vertically injected into the sample tube. At the same time, when there is residual antibody material in the reservoir 1, the operator can pinch the tail of the reservoir 1 and curl the reservoir downward from the edge seal 12 in sequence to discharge the residual antibody inside from the discharge head 11, thereby maximizing the utilization rate of the antibody material. When faced with the use of precious or high-cost antibody materials, its economic efficiency is effectively improved. Finally, during the entire antibody addition operation, the antibody material does not come into contact with the external environment, which greatly ensures that the antibody is not contaminated. At the same time, it also improves the safety of the operator and avoids some special infectious antibodies from contaminating the surrounding environment and threatening the safety of the operator.

[0085] In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise", "axial", "radial", "circumferential" and the like to indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operate in a specific orientation, and therefore should not be understood as a limitation to the invention.

[0086] Throughout this specification, reference to terms such as "one embodiment," "some embodiments," "illustrative embodiments," "example," "specific example," or "some examples" means that a specific feature, structure, material, or characteristic described in conjunction with the embodiment or example is included in at least one embodiment or example of the present invention. In this specification, illustrative expressions of the above terms do not necessarily refer to the same embodiment or example.

[0087] Obviously, the described embodiments are only some of the embodiments of the present application, rather than all of the embodiments. Mentioning "embodiment" in this article means that the specific features, structures or characteristics described in conjunction with the embodiment may be included in at least one embodiment of the present embodiment application. The appearance of this phrase in various positions in the specification does not necessarily mean that they are all the same embodiments, nor are they independent or alternative embodiments that are mutually exclusive with other embodiments. It can be understood explicitly and implicitly by those skilled in the art that the embodiments described herein can be combined with other embodiments. Based on the embodiments in this application, all other embodiments obtained by those of ordinary skill in the art without making creative work are within the scope of protection of this application.

[0088] While embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions, and variations may be made to the embodiments without departing from the principles and spirit of the invention, and that the scope of the invention is defined by the claims and their equivalents.

Claims

1. A closed flow cytometer sample processing device, characterized in that: include: A storage container (1) having a chamber (10) and a discharge head (11), wherein the chamber (10) is in communication with the discharge head (11); A sealing ring membrane (2) is provided for separating the cavity (10) into a first chamber (101) and a second chamber (102) which are not connected to each other. The sealing ring membrane (2) comprises: A sealing body (20) connected to the reservoir (1), wherein the sealing body (20) has a surface that gradually sinks toward the center; A plurality of first open blind seams (21) are provided on the surface of the sealing body (20); A plurality of second opening blind slits (22) are provided on the other surface of the sealing body (20), corresponding to the position of each first opening blind slit (21); The guide slot (23) is provided on the sealing body (20) for quickly breaking the sealing ring membrane (2) and guiding the cell sample to be squeezed out of the discharge head (11) in a vertical jet shape after being pressurized. The sealing body (20) near the center has a straight portion (201), a circular portion (202) and an arc portion (203). The straight portion (201), the circular portion (202) and the arc portion (203) are connected in sequence to form the guide slot (23). The straight portion (201) is located on the extension line of the surface trajectory of the sealing body (20) symmetrical to it. The guide slot (23) and the first opening blind slit (21) are located on the same side, and the angle between the guide slot (23) and the first opening blind slit (21) is in the range of 30° to 90°, so that the position of one guide slot is exactly in the middle position between the two first opening blind slits.

2. The closed flow cytometer sample processing device according to claim 1, characterized in that: The cross-sections of the first opening blind seam (21) and the second opening blind seam (22) are both triangular wedge-shaped, and the depth of the first opening blind seam (21) is greater than the depth of the second opening blind seam (22).

3. The closed flow cytometer sample processing device according to claim 1, characterized in that: The plurality of first opening blind seams (21) are symmetrically distributed about the center of the sealing body (20).

4. The closed flow cytometer sample processing device according to claim 3, characterized in that: The first opening blind seams (21) are distributed in a cross shape.

5. The closed flow cytometer sample processing device according to claim 1, characterized in that: The end of the reservoir (1) away from the discharge head (11) is provided with a sealing edge (12).

6. The closed flow cytometer sample processing device according to claim 1, characterized in that: Also includes: Metal damping wires (5) are installed on both sides of the storage (1).

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