Protein reaction device for full-automatic protein interaction test

Abstract

The invention relates to the field of protein interaction experiments, in particular to a protein reaction device for a full-automatic protein interaction test, which comprises a magnetic part for separating magnetic beads in a sample tube and a support frame arranged on an experimental platform, a reaction table is rotatably arranged on the support frame, a plurality of accommodating holes for inserting the sample tube are formed in the reaction table, and the sample tube is arranged in the accommodating holes. The axes of the containing holes are located in the same plane, a refrigerating module and a heating module are arranged at the positions, corresponding to the containing holes, of the reaction table and used for refrigerating and heating the sample pipes, and a turning cover plate is rotationally connected to the top face of the reaction table and used for opening and closing pipe openings of the sample pipes at the same time. By implementing the scheme, the effects of small and exquisite structure, low cost and automatic operation are achieved.

Description

Technical Field

[0001] The present invention relates to the field of protein interaction experiments, and in particular to a protein reaction device for fully automated protein interaction experiments. Background Art

[0002] Co-IP experiment is co-immunoprecipitation experiment, which is mainly used by scientific researchers to study the interaction between proteins and determine which proteins bind to the target protein to form a complex under physiological conditions in the cell. The characteristics of this experiment are: 1) The experimental process is relatively cumbersome, including but not limited to: magnetic bead pretreatment (resuspending the magnetic beads multiple times), antibody binding (antibody dilution, gentle resuspending of magnetic beads and antibodies and flipping at low temperature overnight), precipitation reaction (resuspending multiple times, flipping at low temperature overnight after protein binding, magnetic separation, resuspending multiple times, high temperature heating, magnetic separation, etc.); 2) The content of each experiment is different, because humans have tens of thousands of proteins and a huge antibody library. Even for the same protein, different designs are required when studying its role in different pathways or diseases. In addition, each experiment requires a new control group (negative control, positive control) to be redesigned for the new target. 3) It takes a long time: one experiment takes scientific researchers nearly 2 days.

[0003] At present, the general situation of scientific researchers conducting this experiment is manual operation. The reasons for this situation are: 1) Due to the second experimental feature of the Co-IP experiment mentioned above, that is, the different experimental content and control groups of each experiment, the relevant parameters cannot be set uniformly; 2) Due to the large number of variables involved in this experiment and the cumbersome operation steps, its R&D cost is high, and the field of experimental equipment is a niche field. Therefore, based on cost-benefit considerations, there is currently no equipment on the market that can realize fully automated protein interaction experiments. However, the personnel of the Academy of Sciences have devoted most of their energy to tedious experimental operations, which greatly compresses their time and energy for innovative research, which is not conducive to scientific and technological progress.

[0004] In response to the above problems, the applicant has developed an automated protein interaction test device that does not require human intervention. Among them, a major difficulty is: in the protein interaction experiment, it is necessary to continuously open the sample tube cover to aspirate and add liquid, and then it is necessary to close the sample tube cover in time for magnetic separation or flip mixing. At the same time, because each sample tube in the same batch of experiments is a control experiment, the sample tubes cannot be cross-flowed, that is, each sample tube must ensure good airtightness, and at the same time, the completion of the current batch of experiments cannot affect the next experiment; in addition, flip mixing, magnetic separation, heating, cooling and other processes are required during the experimental reaction. Therefore, it is necessary to design a protein interaction reaction device with a compact structure, low cost and automation. Summary of the Invention

[0005] The present invention aims to provide a protein reaction device for fully automated protein interaction experiments, so as to achieve the effects of compact structure, low cost and automated operation.

[0006] To achieve the above-mentioned objectives, the present invention adopts the following technical solution: a protein reaction device for fully automated protein interaction experiments, comprising a magnetic part for separating magnetic beads in a sample tube and a support frame arranged on an experimental platform, a reaction table rotatably provided on the support frame, a plurality of receiving holes for inserting sample tubes provided on the reaction table, the axes of each receiving hole being located in the same plane, a cooling module and a heating module being provided at corresponding positions of each receiving hole on the reaction table for cooling and heating the sample tubes, a flip cover being rotatably connected to the top surface of the reaction table, and the flip cover being used to simultaneously open and close the tube openings of each sample tube.

[0007] Preferably, as an improvement, a tube cover plate is rotatably connected to the tube mouth of the sample tube, and a cover protrusion for sealing the tube mouth is provided on the tube cover plate. A cover hole is also provided on the tube cover plate. When the tube mouth is sealed by the tube cover plate, the cover hole is misaligned with the end face of the tube mouth of the sample tube.

[0008] Preferably, as an improvement, the free end of the flip cover plate is provided with a cover opening mechanism corresponding to the cover opening hole, the cover opening mechanism includes a connecting shaft and a cover opening member, the connecting shaft is vertically arranged on the lower surface of the flip cover plate, one end of the cover opening member is rotatably connected to the side wall of the connecting shaft, the gravity of the cover opening member is greater than the rotational friction between the cover opening member and the connecting shaft; when the cover opening member is folded, the maximum transverse width of the cover opening mechanism is smaller than the diameter of the cover opening hole, and when the cover opening member is opened, the maximum transverse width of the cover opening mechanism is greater than the diameter of the cover opening hole.

[0009] Preferably, as an improvement, a limit plate is provided at one end of the connecting shaft away from the flip cover plate, a limit groove is provided on the limit plate, the connection between the cover opening member and the rotating shaft is located in the limit groove, and the contact surface of the limit groove and the cover opening member is an inclined surface.

[0010] Preferably, as an improvement, a limiting protrusion is transversely provided on the outer peripheral wall of the sample tube, and a limiting ring groove for engaging the limiting protrusion is provided in the accommodating hole.

[0011] Preferably, as an improvement, at least one positioning guide strip is provided on the outer side wall of the sample tube, and the positioning guide strip is vertically arranged, and a positioning guide groove for inserting the positioning guide strip is vertically provided in the accommodating hole.

[0012] Preferably, as an improvement, a heating module is also provided in the flip cover.

[0013] Preferably, as an improvement, the magnetic member is embedded in the side wall of the reaction table corresponding to each receiving hole, and the magnetic member is an electromagnet.

[0014] Preferably, as an improvement, the reaction table is detachably connected to the support frame.

[0015] The design principles of this solution are as follows:

[0016] To design a sample tube cover that effectively avoids the risk of cross-contamination and automatically and uniformly opens and closes each sample tube cover simultaneously, the initial consideration was to use sample tubes with threaded covers, using two opposing racks to twist the cover. While this method could open all sample tubes at once, it presented several challenges. First, a mechanism was required on the reaction table to clamp and release the sample tubes to prevent them from falling during rotation. Second, the rack twisting mechanism was complex, requiring a drive mechanism to operate the racks, a lifting mechanism for the cover to spiral upwards or downwards, and a translation mechanism to horizontally move the entire rack twisting mechanism or cover above the sample tubes. Consequently, the entire structure was complex, requiring numerous operational steps and resulting in high costs.

[0017] Then, we considered using a flip-up plate method, directly setting a cover for sealing each sample tube on the flip-up plate. This method only requires driving the flip-up plate to rotate, and its structure and operation are extremely simple. However, there is a problem: when conducting the next batch of protein interaction experiments, the residual substances on the cover will affect the results of the next experiment. Later, we considered sticking a disposable sealing film on the flip-up plate, and setting multiple covers on the sealing film corresponding to each sample tube. In this way, after each experiment is completed, the sealing film can be torn off and a new sealing film can be pasted. However, the problem that may exist in this method is that manual pasting cannot ensure the accuracy of its position, resulting in the sample tube being not tightly sealed during the experiment and causing the reagent to leak. To address this problem, we later considered using an automated pasting method, setting the sealing film into a roll and storing it in the experimental chamber. When pasting, we need to consider tearing off the adhesive film on the sealing film, then cutting the sealing film of appropriate length and sending it to the designated position, and then the pasting mechanism, with the assistance of the positioning mechanism, accurately pastes the sealing film. Although this method is feasible, it involves additional consumables: sealing film; at the same time, the additional mechanisms involved are too complicated, and the precision requirements required for coordination in the middle are too high, making the overall cost too high, which is not conducive to the original design intention of making the equipment compact and affordable.

[0018] Finally, after continuous analysis and optimization of the structure, this solution was designed, which has the following effects:

[0019] 1. The same structural design can achieve multiple effects at the same time: This solution uses the ingenious design of the flip plate structure to enable the flip plate to achieve four effects at the same time: 1) It can realize batch opening and closing of each sample tube without generating excess consumables; at the same time, the control process is simple, and only the rotation of the flip plate is required to drive the tube cover to open and seal the sample tube mouth. 2) The flip plate can also serve the purpose of pressing the sample tube to prevent the sample tube from being thrown out during the flipping and mixing process. 3) During the flipping process, the flip plate can always press the cover convex and the sample tube to keep the two in a sealed state, further ensuring that the risk of liquid spillage is avoided. 4) Since the sample tube will have residual liquid on the cover convex after the flipping and mixing step, especially in the washing step, the suspension needs to be heated at high temperature. At this time, a large amount of liquid will condense on the cover convex. During the opening process, the liquid on the cover convex is very likely to splash into the adjacent sample tube, resulting in inaccurate experimental results. Therefore, this solution uses a flip cover plate and directly sets a heating module inside it to heat the cover convex of the sample tube to keep the cover convex dry. In this way, no liquid splashing will occur when the cover is opened, ensuring the accuracy of the experimental results.

[0020] 2. The cover opening structure is extremely simple and low-cost: It only consists of a flip cover plate, a connecting shaft and a cover opening piece, which can realize the opening and closing functions of the sample tube and the multiple functions mentioned above. During the process, no additional consumables or unnecessary complex structures are required, making the entire structure extremely simple and the cost extremely low.

[0021] 3. The changes to the sample tube are minimal. Only a cover opening hole is opened on the tube cover to cooperate with the cover opening mechanism to perform automated, batch opening and closing operations. At the same time, in terms of processing technology, the improved sample tube does not increase the processing difficulty compared to the traditional sample tube, so the productization degree is high and the promotion is strong.

[0022] 4. Compact structure and high integration: This solution integrates the heating module, cooling module, magnetic parts and lid opening mechanism on the reaction table, making the entire device compact and neat. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 This is an overall diagram of the experimental equipment including the protein reaction device.

[0024] Figure 2 It is a structural schematic diagram of the present invention.

[0025] Figure 3 This is a schematic diagram of the improved sample tube structure of the present invention.

[0026] Figure 4 A cross-sectional view of the reaction table.

[0027] Figure 5 for Figure 4 A partial enlarged view of middle A.

[0028] Figure 6 for Figure 4 Cross-sectional view of the middle reaction station with the cover opened. DETAILED DESCRIPTION

[0029] The following is further described in detail through specific implementation methods:

[0030] The figure marks in the drawings of the specification include: experimental platform 1, support frame 2, reaction table 3, accommodating hole 4, magnetic part 5, thermoelectric semiconductor cooling plate 6, flip cover plate 7, opening mechanism 8, connecting shaft 9, opening member 10, limiting plate 11, weight reduction hole 12, sample tube 13, tube cover plate 14, cover protrusion 15, opening hole 16, limiting protrusion 17, positioning guide strip 18.

[0031] The embodiment is basically as shown in the attached Figures 1-6 As shown: A protein reaction device for fully automated protein interaction experiments, including a magnetic member 5 for separating magnetic beads in a sample tube 13 and a support frame 2 provided on an experimental platform 1, a reaction table 3 is rotatably provided on the support frame 2, and in this solution, the reaction table 3 is driven by a micro speed-regulating motor, which is fixedly mounted on the support frame 2, and its output shaft is clamped on the reaction table 3. By adopting a micro speed-regulating motor, the experimenter can adjust the speed of the reaction table 3 according to different needs to achieve the ideal experimental effect. A plurality of receiving holes 4 for accommodating sample tubes 13 are provided on the reaction table 3, and each sample tube 13 is used for experiments with different control groups. The number of receiving holes 4 can be 4, 8, 12, etc., and the axes of each receiving hole 4 are located in the same plane. In this embodiment, four receiving holes 4 are taken as an example.

[0032] like Figure 4 As shown, the magnetic member 5 can be installed on the magnetic frame and externally installed on the experimental platform 1. By changing the position of the magnetic member 5, it is controlled whether it absorbs the magnetic beads in the sample tube 13; in addition, the magnetic member 5 can also be vertically embedded in the reaction table 3 and corresponding to the side wall of each receiving hole 4. The magnetic member 5 is an electromagnet. The latter is adopted in this embodiment. This method has a high degree of integration and makes the entire structure compact and simple. At the same time, it can reduce the magnetic frame and the related structures that drive the movement of the magnetic frame, and avoid interference with other components during its movement. In addition, using an electromagnet as the magnetic member 5, in addition to controlling the working state of the electromagnet by turning the power on and off, the magnitude of its magnetic force can also be controlled by changing the current to be suitable for different types of magnetic beads. In addition, in this solution, the electromagnet is vertically located on the bottom side wall of the sample tube 13. Its purpose is to adsorb the magnetic beads on the side wall of the sample tube 13 rather than the bottom of the tube, so as to avoid the risk of the magnetic beads being sucked away when the pipette tip is inserted into the bottom of the sample tube 13 to absorb the liquid.

[0033] Since, in the antibody binding step of the experiment, the liquid in the sample tube 13 needs to be turned over overnight with the reaction table 3 under low temperature conditions (usually at 4°C), it needs to be refrigerated. At the beginning of the design, constant temperature refrigeration was considered in the experimental chamber, because in addition to the liquid in the sample tube 13, there are various other reaction liquids stored in the experimental chamber that also need to be stored at low temperatures, but it was later discovered that this method will cause water mist and even condensed water droplets to form in the experimental chamber, especially on the pipette gun, and there is a high possibility of contamination risk during the pipetting process. Therefore, it was later considered to set a refrigeration module at each receiving hole 4 on the reaction table 3, which not only avoids the generation of water droplets in the experimental chamber, but also saves energy and reduces unnecessary low-temperature consumption in the experimental chamber.

[0034] At the same time, in the washing step of the final stage of the experiment, the suspension needs to be heated at a high temperature (usually at 95°C), so a heating module is set up on the reaction table 3 corresponding to each receiving hole 4. The heating module and the cooling module can be set up using a heating layer and a cooling layer respectively. However, considering that the space of the reaction table 3 is limited and it needs to be in a flipped state for a long time, the reaction table 3 as a whole needs to be light and compact. After comprehensive consideration, a thermoelectric semiconductor cooling plate 6 with both cooling and heating functions is adopted. It can achieve cooling and heating function switching and temperature adjustment by simply changing the direction and size of the current. At the same time, the thermoelectric semiconductor cooling plate 6 has a small structure and can meet the installation space corresponding to a single sample tube 13. The installation position of the thermoelectric semiconductor cooling plate 6 is offset from the position of the magnetic part 5.

[0035] A number of weight-reducing holes 12 are provided on the reaction table 3, some of which are connected to the thermoelectric semiconductor cooling plate 6. This can not only reduce the weight of the entire reaction table 3, but also facilitate the heat dissipation of the thermoelectric semiconductor cooling plate 6 when heated.

[0036] Since in the entire magnetic bead protein interaction experiment, it is necessary to continuously open the cover of the sample tube 13 for aspiration and addition of liquid, and then close the cover of the sample tube 13 in time for magnetic separation or flipping and mixing. At the same time, since in the same batch of experiments, each sample tube 13 is a control experiment, the sample tubes 13 cannot be cross-flowed, that is, each sample tube 13 must ensure good airtightness, and at the same time, the completion of the current batch of experiments cannot affect the next experiment. Therefore, how to design a sample tube 13 cover that can effectively avoid the risk of mutual contamination and can automatically and uniformly open and close the cover of each sample tube 13 at the same time is a very important and critical link.

[0037] Therefore, after continuous analysis and optimization of the structure, the following structure is finally designed:

[0038] First, if Figure 3As shown, the sample tube 13 is improved: a tube cover 14 is rotatably connected to the tube mouth of the sample tube 13. Tube cover 14 is provided with a cover protrusion 15 for sealing the tube mouth. Cover protrusion 15 can be solid or hollow, but this embodiment adopts a hollow design to save materials and costs. The peripheral wall of cover protrusion 15, located three-quarters of the way away from tube cover 14, is designed as an inclined surface, facilitating easy insertion of cover protrusion 15 into the sample tube 13. Tube cover 14 also has a cover opening 16. When tube cover 14 is sealing the tube mouth, cover opening 16 is offset from the end face of the tube mouth of the sample tube 13. The main improvement of sample tube 13 over conventional sample tubes 13 lies in the simple addition of cover opening 16 to tube cover 14.

[0039] Secondly: Figure 4-Figure 6 As shown, a flap plate 7 is rotatably connected to the top surface of the reaction table 3 via a bearing. The flap plate 7 is used to simultaneously open and close the nozzles of each sample tube 13. In this embodiment, the flap plate 7 is driven by a servo motor fixed to the reaction table 3, and its output shaft is connected to the flap plate 7. A lid opening mechanism 8 is vertically mounted on the left end of the flap plate 7, corresponding to the position of the lid opening hole 16. The lid opening mechanism 8 includes a connecting shaft 9 and two lid opening members 10. The connecting shaft 9 is vertically mounted on the bottom surface of the flap plate 7. The lower ends of the lid opening members 10 are hinged to the lower end of the connecting shaft 9 via a pin. In this embodiment, the lower ends of the lid opening members 10 are U-shaped and rotatably connected to the connecting shaft 9 via a pin. The gravity of the lid opening members 10 is greater than the rotational friction between the lid opening members 10 and the connecting shaft 9. This allows the upper ends of the lid opening members 10 to automatically expand outward under their own weight in the absence of external forces. When the two cover opening members 10 are folded together, the maximum transverse width of the cover opening mechanism 8 is smaller than the diameter of the cover opening hole 16, so that the cover opening mechanism 8 can be smoothly inserted into the cover opening hole 16 of the sample tube 13; when the cover opening members 10 are unfolded, the maximum transverse width of the cover opening mechanism 8 is larger than the diameter of the cover opening hole 16, so that during the cover opening process, the cover opening members 10 contact the bottom surface of the flip cover plate 7 and directly drive the flip cover plate 7 to rotate upward until the cover is opened.

[0040] The lower end of the connecting shaft 9 is integrally formed with a limit plate 11. A limit groove is defined in the middle of the limit plate 11. The connection between the cover opening member 10 and the rotating shaft is located within the limit groove. The contact surface between the limit groove and the cover opening member 10 is an inclined surface. This design not only limits the expansion stroke of the cover opening member 10, but also effectively supports the cover opening member 10 during the opening process, thereby extending the service life of the cover opening mechanism 8.

[0041] As a more optimal improvement, an electromagnet rod is installed in the connecting shaft 9, and the cover opening member 10 is made of iron or an iron sheet is provided on the cover opening member 10. In this way, when the experimental accuracy is high and the sample tube 13 needs to be replaced, the electromagnet rod in the connecting shaft 9 is energized and magnetized, thereby attracting and retracting the cover opening member 10. The flip cover plate 7 flips upward, disengaging the cover opening mechanism 8 from the tube cover plate 14 of the sample tube 13. At this time, the sample tube 13 is in a free state, and the robot arm removes the sample tube 13 from the reaction table 3 and transfers the liquid in the tube to a new sample tube 13.

[0042] To prevent the lid-opening mechanism 8 from pulling the sample tube 13 out during the opening process, a horizontal stopper protrusion 17 is provided on the outer wall of the sample tube 13, and a stopper groove is provided within the receiving hole 4 to engage with the stopper protrusion 17, thereby securing the two. Furthermore, to ensure precise alignment between the lid-opening hole 16 of the sample tube 13 and the lid-opening mechanism 8, allowing the lid-opening mechanism 8 to be accurately inserted into the lid-opening hole 16, at least one positioning guide bar 18 is integrally formed on the outer wall of the sample tube 13. This positioning guide bar 18 extends axially along the sample tube 13, and a vertical positioning guide groove is provided within the receiving hole 4 for the positioning guide bar 18 to insert. This allows the experimenter to insert the positioning guide bar 18 into the corresponding positioning guide groove when placing the sample tube 13, ensuring a one-to-one alignment between the lid-opening hole 16 and the lid-opening mechanism 8. Furthermore, the top of the positioning guide groove is flared, with the opening facing upward, to simplify alignment.

[0043] Considering that after the sample tube 13 has gone through the flipping and mixing step, liquid will remain on the cover bulge 15. Especially in the washing step, the suspension needs to be heated at high temperature. At this time, a large amount of liquid will condense on the cover bulge 15. During the opening process, the liquid on the cover bulge 15 is very likely to splash into the adjacent sample tube 13, thereby causing inaccurate experimental results.

[0044] Therefore, in this solution, a heating module, which can be an electric heating wire, is installed in the flip cover 7. After the sample tube 13 is flipped and mixed, the cover ridge 15 is heated to evaporate the water vapor or small water droplets condensed on the cover ridge 15, ensuring that the surface of the cover ridge 15 is dry and preventing liquid splashing when the cover is opened.

[0045] In order to meet the more diverse experimental demands of experimenters, sample tubes 13 of different capacities will be used. Therefore, reaction tables 3 with various specifications of accommodating holes 4 are designed for sample tubes 13 of different specifications, and the reaction tables 3 can be detachably connected to the support frame 2, such as by snap connection, bolt connection, etc.

[0046] The specific operation method is as follows:

[0047] According to the specifications of the sample tube 13, the corresponding reaction table 3 is selected and installed on the support frame 2 and the micro speed-regulating motor. The experimenter aligns the positioning guide strip 18 of the sample tube 13 with the positioning guide groove and inserts it into the receiving hole 4. At this time, the opening hole 16 on the sample tube 13 corresponds to the opening mechanism 8 on the flip plate 7, and the protein interaction experiment begins. When it is necessary to seal the sample tube 13 and start the flip plate 7 to rotate downward, the opening mechanism 8 is inserted into the corresponding opening hole 16. Since the lower end of the opening member 10 is rotatably connected to the connecting shaft 9, when the opening member 10 is inserted, the upper end of the opening member 10 automatically retracts inward. After the opening member 10 passes through the opening hole 16, the upper end of the opening member 10 automatically expands outward under the action of its gravity. When it is necessary to open the cover, the flip plate 7 rotates upward, and the opening member 10 drives the tube cover plate 14 to move upward until it is pulled out, thereby achieving the purpose of opening the cover. When magnetic separation is required during this period, magnetic attraction is performed by turning on the electromagnet. When the experiment is completed, the experimenter only needs to press the cover opening member 10 to close it, start the flip cover plate 7 to flip upward and separate from the cover hole 16, and then the experimenter can remove the sample tube 13.

[0048] The above is only an embodiment of the present invention, and the common knowledge such as the specific technical solutions and / or characteristics in the solution are not described in detail here. It should be pointed out that for those skilled in the art, without departing from the technical solution of the present invention, several variations and improvements can be made, which should also be regarded as the scope of protection of the present invention, and these will not affect the effect of the implementation of the present invention and the practicality of the patent. The scope of protection required by this application shall be based on the content of its claims, and the specific implementation methods and other records in the description can be used to interpret the content of the claims.

Claims

1. A protein reaction device for fully automated protein interaction experiments, characterized by: It includes a magnetic part for separating magnetic beads in a sample tube and a support frame arranged on the experimental platform. A reaction table is rotatably provided on the support frame. The reaction table is provided with multiple accommodating holes for inserting sample tubes. The axes of each accommodating hole are located in the same plane. A cooling module and a heating module are provided at each accommodating hole corresponding to each accommodating hole, which are used to cool and heat the sample tube. The top surface of the reaction table is rotatably connected to a flip cover plate, which is used to simultaneously open and close the tube openings of each sample tube.

2. A protein reaction device for fully automated protein interaction assay according to claim 1, characterized in that: The tube mouth of the sample tube is rotatably connected to a tube cover plate, which is provided with a cover protrusion for sealing the tube mouth and a cover opening hole. When the tube cover plate is in a state of sealing the tube mouth, the cover opening hole is misaligned with the end face of the tube mouth of the sample tube.

3. A protein reaction device for fully automated protein interaction assay according to claim 2, characterized in that: The free end of the flip cover plate is provided with a cover opening mechanism corresponding to the cover opening hole, the cover opening mechanism comprising a connecting shaft and a cover opening member, the connecting shaft being vertically arranged on the lower surface of the flip cover plate, one end of the cover opening member being rotatably connected to the side wall of the connecting shaft, and the gravity of the cover opening member being greater than the rotational friction between the cover opening member and the connecting shaft; When the cover opening member is folded, the maximum transverse width of the cover opening mechanism is smaller than the diameter of the cover opening hole; when the cover opening member is opened, the maximum transverse width of the cover opening mechanism is larger than the diameter of the cover opening hole.

4. A protein reaction device for fully automated protein interaction assay according to claim 3, characterized in that: A limiting plate is provided at one end of the connecting shaft away from the flip cover plate, and a limiting groove is provided on the limiting plate. The connection between the cover opening member and the rotating shaft is located in the limiting groove, and the surface where the limiting groove contacts the cover opening member is an inclined surface.

5. A protein reaction device for fully automated protein interaction assay according to claim 4, characterized in that: A limiting protrusion is transversely provided on the outer peripheral wall of the sample tube, and a limiting ring groove for engaging the limiting protrusion is provided in the accommodating hole.

6. A protein reaction device for fully automated protein interaction assay according to claim 5, characterized in that: At least one positioning guide strip is provided on the outer side wall of the sample tube. The positioning guide strip is vertically arranged. A positioning guide groove for inserting the positioning guide strip is vertically provided in the accommodating hole.

7. A protein reaction device for fully automated protein interaction assay according to claim 6, characterized in that: A heating module is also provided in the flip cover.

8. A protein reaction device for fully automated protein interaction assay according to claim 7, characterized in that: The magnetic piece is embedded in the side wall of the reaction table corresponding to each receiving hole, and the magnetic piece is an electromagnet.

9. A protein reaction device for fully automated protein interaction assay according to claim 8, characterized in that: The reaction platform is detachably connected to the support frame.

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