Method for evaluating human health by airborne pollutants

Abstract

The invention discloses a method for evaluating human health by airborne pollutants, and belongs to the technical field of pollutant evaluation, and the method comprises the following steps: S1, simulating a real exposure environment, constructing an airborne pollutant exposure simulation system, S2, systematically monitoring multiple organ indexes, and systematically monitoring pollutants, and S3, carrying out interdisciplinary comprehensive analysis and evaluation. A research team formed by environmental science, medicine and molecular biology multidisciplinary professionals is established, key organ damage is analyzed, the influence of pollutants on human health is comprehensively evaluated, the research design is close to the real environment by simulating the real exposure environment, the whole process of the pollutants from exposure to excretion can be simulated, and the real exposure environment is simulated. A scientific basis is provided for evaluating the health risk of the pollutants, meanwhile, through the propagation path of the pollutants in the human body, multiple organ damages are connected in series, a systematic research framework is formed, and the limitation of single organ analysis in traditional research is avoided.

Description

Technical Field

[0001] The present invention relates to the technical field of pollutant assessment, and more particularly to a method for assessing the effects of airborne pollutants on human health. Background Art

[0002] Among the existing assessment technologies for the effects of airborne pollutants on human health, ecological relevance is insufficient. Most of them use traditional intravenous injection and nasal drip methods, which may not accurately simulate the pollutant exposure conditions in the real atmospheric environment, resulting in deviations between research results and actual health risks. In addition, existing research mostly focuses on the toxic effects of a single organ and lacks a systematic analysis of the transmission pathways of pollutants in the body and their multi-organ damage. At the same time, existing research methods are mostly concentrated in a single discipline and lack interdisciplinary integration. Summary of the Invention

[0003] The present invention mainly provides a method for evaluating the effects of airborne pollutants on human health, which can solve the problems of insufficient ecological relevance, lack of systematic perspective, and lack of interdisciplinary integration raised by the above-mentioned background technology.

[0004] To achieve the above object, the present invention provides the following technical solution: a method for evaluating the effects of airborne pollutants on human health, comprising the following steps: S1. Simulate real exposure environment and build airborne pollutant exposure simulation system; S2. Systematically monitor multiple organ indicators and pollutants; S3. Conduct interdisciplinary comprehensive analysis and assessment, forming a research team composed of multidisciplinary professionals in environmental science, medicine, and molecular biology to analyze damage to key organs and comprehensively assess the impact of pollutants on human health.

[0005] Furthermore, the simulation system in S1 utilizes an airborne nanoplastic exposure device, a nanoplastic aerosol generator, a nanoplastic exposure chamber, an air pump, and an exhaust gas collection device to construct a pollutant exposure scenario close to the real atmospheric environment, and places the experimental subjects in the nanoplastic exposure chamber for simulation, thereby overcoming the problem that traditional intravenous injection, nasal drip and other methods cannot accurately simulate the exposure of pollutants in the real atmospheric environment, and making up for the defect of insufficient ecological relevance.

[0006] Furthermore, the multiple organs in S2 include the heart, lungs, liver, spleen, brain, and kidneys, and the systematic monitoring of pollutants is divided into the entry stage, the transmission stage, the excretion stage, and the renal toxicity stage.

[0007] Furthermore, the monitoring during the entry phase requires exposing the experimental subjects to the airborne pollutant exposure simulation device in S1, and closely monitoring respiratory-related indicators through respiratory monitoring equipment to evaluate the initial impact of pollutants on the respiratory tract and clarify the situation of pollutants entering the body from the respiratory system.

[0008] Furthermore, the monitoring during the transmission stage is to monitor the organs that are preferentially affected after the pollutants enter the body. These organs include the liver, spleen, and brain. The liver, spleen, and brain are selected as the research focus because these organs play a key role in metabolism, immunity, and neural function, and can comprehensively reflect the toxicity mechanism of pollutants.

[0009] Furthermore, the excretion and renal toxicity stage monitoring is because pollutants are ultimately excreted from the body through the urinary system. The kidneys, as the main excretory organ, are responsible for filtering harmful substances in the blood, and therefore become another important target for pollutant accumulation and toxic effects.

[0010] Furthermore, the multidisciplinary personnel in S3 jointly integrate data to analyze the transmission paths of pollutants in the body, the damage mechanisms to various organs, and the intrinsic connections between the damage to various organs, comprehensively evaluate the comprehensive impact of airborne pollutants on human health, and provide a basis for formulating targeted prevention and control measures.

[0011] Furthermore, the S1 uses a laser scattering particle size analyzer to monitor the nanoplastic aerosol particle size in real time, and automatically adjusts the nanoplastic aerosol generator parameters through a feedback control system to stabilize the nanoplastic aerosol particle size within the target range of ±10nm.

[0012] Furthermore, during the S2 stage monitoring, a nanotechnology-based respiratory sensor array is used to detect the concentrations of five different types of airborne pollutants, with a detection limit as low as 0.1μg / m³, so as to more accurately assess the entry of pollutants into the body; when monitoring the liver during the transmission stage, a liver cell gene knockout or overexpression model is constructed through gene editing technology to study the mechanism of action of specific genes in the effects of pollutants on liver metabolism and detoxification function; when monitoring the spleen, proteomics and phosphoproteomics technologies are combined to comprehensively analyze the changes in protein expression and phosphorylation modification of splenic immune cells under the action of pollutants, and identify key proteins and modification sites related to abnormal immune regulation; when monitoring the brain, optogenetics technology is used to specifically regulate the activity of specific neurons in the brain, and combined with in vivo multi-electrode array recording technology, the effects of pollutant exposure on neuronal electrical activity and neural circuit function are studied; when monitoring the excretion and renal toxicity stages, a kidney organoid model based on a microfluidic chip is used to simulate the physiological function of the kidney, and the toxic effects of pollutants on kidney cells are monitored in real time on the chip.

[0013] Furthermore, in the S3 interdisciplinary comprehensive analysis and evaluation, systems biology methods were used to construct a network model of the interaction between airborne pollutants and human health, integrating multi-omics data to comprehensively analyze the molecular mechanisms and network regulatory relationships of the impact of pollutants on human health.

[0014] The beneficial effects of the method for evaluating the effects of airborne pollutants on human health provided by the present invention are as follows: By simulating the real exposure environment, the research design is close to the real environment, and the entire process from exposure to excretion of pollutants can be simulated, providing a scientific basis for evaluating the health risks of pollutants. At the same time, through the transmission path of pollutants in the human body, multiple organ damage is connected in series to form a systematic research framework, avoiding the limitations of single organ analysis in traditional research. In addition, multidisciplinary personnel jointly integrate data to analyze the transmission path of pollutants in the body, the damage mechanism to various organs, and the inherent connection between the damage to various organs, comprehensively evaluate the comprehensive impact of airborne pollutants on human health, and provide a basis for formulating targeted prevention and control measures. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] The present invention will be further described in detail below with reference to the accompanying drawings and specific implementation methods.

[0016] Figure 1 The figure is a flow chart of a method for evaluating the effects of airborne pollutants on human health according to the present invention. DETAILED DESCRIPTION

[0017] In order to make the technical solution of the present invention clearer, the present invention is further described in detail below with reference to the accompanying drawings and specific embodiments. Example

[0018] like Figure 1 As shown, a technical solution is provided: a method for evaluating the effects of airborne pollutants on human health, comprising the following steps: Step 1: Simulate the real exposure environment and build an airborne pollutant exposure simulation system; The simulation system integrates an airborne nanoplastic exposure device, a nanoplastic aerosol generator, a nanoplastic exposure chamber, an air pump, and an exhaust gas collection device to construct a pollutant (airborne nanoplastic) exposure scenario that highly simulates the real atmospheric environment. Specifically, the system places experimental animals in the nanoplastic exposure chamber, while precisely controlling key parameters such as the concentration and particle size of airborne pollutants, thereby effectively simulating the human body's exposure process in the actual environment. Compared with traditional experimental methods such as intravenous injection and nasal instillation, this simulation system can more accurately restore the exposure of pollutants in the real atmospheric environment, successfully making up for the lack of ecological relevance of traditional methods. At the same time, the laser scattering particle size analyzer emits a laser beam to the nanoplastic aerosol. The particles in the aerosol scatter the laser. The instrument calculates the particle size based on the angle and intensity distribution of the scattered light using a specific algorithm. At the same time, it is connected to the feedback control system. When the particle size of the nanoplastic aerosol is monitored to deviate from the target range of ±10nm, the feedback control system automatically adjusts the parameters of the nanoplastic aerosol generator, such as the ultrasonic frequency and solution concentration (for example, if the particle size is too large, the feedback control system will reduce the ultrasonic frequency to make the generated aerosol particle size smaller; if the particle size is too small, the solution concentration will be appropriately increased to increase the particle size), ensuring that the particle size of the nanoplastic aerosol is stable within the target range, more accurately simulating the particle size distribution of the nanoplastic aerosol in the real atmospheric environment, and providing reliable exposure conditions for subsequent experiments.

[0019] Step 2: Systematically monitor multiple organ indicators. Conduct systematic monitoring of pollutants. Multiple organs include the heart, lungs, liver, spleen, brain, and kidneys. Systematic monitoring of pollutants is divided into the entry stage, transmission stage, excretion stage, and renal toxicity stage. Monitoring during the entry phase requires exposing the subjects to the airborne pollutant exposure simulation device in S1. Respiratory monitoring equipment (such as a spirometer) is used to closely monitor respiratory indicators (such as heart rate, blood pressure, electrocardiogram, and vital capacity) to assess the initial impact of pollutants on the respiratory tract, clarify the entry of pollutants into the body through the respiratory system, and observe whether airborne pollutants induce cardiopulmonary diseases when entering the body (such as whether they cause heart failure by affecting the PI3K / AKT signaling pathway). During the transmission phase, monitoring focuses on the organs that pollutants preferentially affect after entering the human body. The liver, spleen, and brain were selected as key research targets because they play key roles in metabolism, immune regulation, and neurological function, respectively. Monitoring these organs allows for a comprehensive analysis of the toxic mechanisms of pollutants, providing important evidence for a deeper understanding of their impact on human health. Before the initial monitoring phase, monitoring was conducted using a nanotechnology-based breath sensor array. This array consists of multiple sensors modified with nanomaterials. Carbon nanotube-modified sensors are sensitive to organic pollutants, while zinc oxide nanowire-modified sensors are highly sensitive to inorganic pollutants. Once airborne pollutants come into contact with the corresponding nanomaterial-modified sensors, their electrical or optical properties change, enabling the detection of pollutant concentrations. This breath sensor array can simultaneously measure the concentrations of five different types of airborne pollutants with a detection limit as low as 0.1 μg / m³. During the experiment, the sensor array was connected to the subject's respiratory device to collect real-time pollutant concentration data. Combined with respiratory-related indicators such as heart rate, blood pressure, electrocardiogram, and vital capacity obtained from respiratory monitoring devices such as spirometers, this allows for a more accurate assessment of the pollutant's initial impact on the respiratory tract and its entry into the body, providing comprehensive data support for in-depth analysis of the pollutant's subsequent effects in the body. During the transmission phase, we employed a multi-dimensional approach to comprehensively assess the impact of airborne pollutants on liver metabolism and detoxification.

[0020] On the one hand, blood samples are collected from experimental subjects at regular intervals to detect liver function indicators and indicators related to glucose and lipid metabolism. By testing liver function indicators such as alanine aminotransferase, aspartate aminotransferase, and bilirubin in the blood, the metabolic function of the liver can be directly assessed. At the same time, glucose, lipid, and insulin levels and other glucose and lipid metabolism-related indicators are tested to determine whether the experimental subjects have insulin resistance, glucose and lipid metabolism disorders, brown fat whitening, etc. In addition, the expression of specific genes (such as FoxO1, Pek1, G6pc, Srebp-1c, Acc, Fas, etc.) is also tested to analyze the impact of pollutants on liver metabolism-related genes from a holistic perspective. This blood sample-based detection method can reflect the overall functional changes and metabolic status of the liver after exposure to pollutants at a macro level. On the other hand, gene editing technology is used to construct gene knockout or overexpression models in liver cells to further study the mechanism of action of specific genes in the effects of pollutants on liver metabolism and detoxification. Taking the Nrf2 gene related to oxidative stress as an example, the specific operation is as follows: first, a guide RNA (gRNA) specific to the Nrf2 gene is designed and co-transfected into liver cells with a Cas9 nuclease vector. The gRNA guides the Cas9 nuclease to recognize and cut the specific target site of the Nrf2 gene, achieving gene knockout. To construct an overexpression model, an expression vector containing the complete coding sequence of the Nrf2 gene and a strong promoter is transfected to overexpress the Nrf2 gene. The constructed gene knockout or overexpression liver cell models are exposed to airborne pollutants, and cell samples are collected regularly for testing. The testing content includes changes in intracellular antioxidant enzyme activity, metabolism-related gene expression, and pollutant detoxification ability. For example, the activity of antioxidant enzymes such as glutathione peroxidase and superoxide dismutase is tested, the expression levels of lipid metabolism-related genes (such as FoxO1, Pck1, G6pc, etc.) are analyzed, and the excretion of pollutant metabolites by the cells is observed. Through this cellular level research, the specific role of specific genes (such as Nrf2) in the process of pollutants affecting liver metabolism and detoxification function can be deeply understood; Combining the above two monitoring methods, macroscopic detection based on blood samples can reveal the overall functional and metabolic abnormalities that may occur in the liver after exposure to pollutants, while cell model research based on gene editing technology can deeply explore the genetic level mechanisms behind these abnormalities. The two complement each other and provide richer and more accurate information for a comprehensive and in-depth assessment of the impact of airborne pollutants on the liver.

[0021] When monitoring the spleen during the transmission phase, a variety of technical methods were used to comprehensively explore the impact of pollutants on the spleen's immune regulatory mechanisms: First, traditional monitoring methods are used, using ultrasound and other imaging methods to closely monitor changes in spleen size and morphology. Ultrasound examinations can clearly show the spleen's outline, boundaries, and internal structure. Regular inspections can promptly detect abnormalities such as spleen atrophy, providing a direct basis for assessing the physical impact of pollutants on the spleen. At the same time, the number and activity of immune cells in the blood are tested, such as the classification and counting of lymphocyte subsets to understand the changes in the number of different subsets such as T lymphocytes and B lymphocytes; and the evaluation of macrophage function, including its phagocytic ability and cytokine secretion capacity. These tests can reflect the immune function status of the spleen at the immune cell level and determine whether the immune function is disordered. In addition, the expression of genes related to immune regulation (Plcg2, Cd247, etc.) was detected. Using real-time fluorescence quantitative PCR and other technologies, the expression changes of these genes under the influence of pollutants were analyzed to preliminarily explore the impact of pollutants on the immune regulation mechanism of the spleen at the genetic level. On this basis, further research was conducted by combining proteomics and phosphoproteomics. First, total protein from splenic immune cells was extracted and then proteomic analysis was performed using two-dimensional liquid chromatography-mass spectrometry. This technology can efficiently separate and identify a large number of proteins, accurately determine changes in their expression levels, and reveal changes in splenic immune cells under the influence of pollutants at the global protein level. At the same time, methods such as immunoaffinity chromatography are used to enrich phosphorylated proteins, as protein phosphorylation plays a key role in cell signal transduction and immune regulation. The enriched phosphorylated proteins are then analyzed by mass spectrometry to identify phosphorylation sites and changes in modification levels. In-depth analysis of proteomics and phosphoproteomics data can identify key proteins and modification sites associated with abnormal immune regulation. For example, studies have found that the phosphorylation sites of certain proteins, such as STAT3, change in response to pollutants. STAT3 is a key molecule in the immune signaling pathway, and changes in its phosphorylation sites can affect its interactions with key molecules in downstream immune signaling pathways, thereby influencing processes such as immune cell activation, proliferation, and cytokine secretion. This ultimately reveals the underlying mechanisms by which pollutants regulate splenic immunity. By combining traditional monitoring methods with proteomics and phosphoproteomics technologies, the spleen under the influence of pollutants was studied from multiple levels and at different depths, comprehensively revealing the impact of pollutants on the spleen's immune regulation mechanism, providing sufficient basis for a deeper understanding of the impact of pollutants on the immune system.

[0022] When monitoring the brain during the transmission phase, various technologies are used to explore the impact of pollutants on the brain from different dimensions: First, neuroimaging techniques such as MRI and PET are used to observe changes in brain structure and function. Behavioral tests (maze and open field tests) are then used to assess the subject's behavior. Simultaneously, the expression of neuroinflammatory markers (such as UCHL1 and GFAP) and genes related to neural function (such as Pvalb and Gad2) in the cerebrospinal fluid or blood is tested to determine whether there are abnormalities such as brain inflammation and neurodegeneration. On this basis, further research is conducted using optogenetics and in vivo multi-electrode array recording technology. Genes encoding light-sensitive ion channels (such as Channelrhodopsin-2) are transfected into specific neuronal populations in the brain, and neuronal activity is modulated by irradiation with light of a specific wavelength. In vivo multi-electrode arrays are implanted to record neuronal electrical activity in real time, comparing changes in neuronal electrical activity under light stimulation before and after pollutant exposure. Abnormal information transmission in neural circuits is observed, and the effects of pollutants on neuronal electrical activity and neural circuit function are comprehensively analyzed, revealing in depth the neural mechanisms by which pollutants affect brain function. Neuroimaging, behavioral tests, and biomarker detection reflect the macroscopic manifestations and physiological changes of the brain affected by pollutants at a holistic level. Optogenetics and in vivo multi-electrode array recording technology penetrate into the microscopic level of neurons and neural circuits, revealing the neuroelectrophysiological mechanisms by which pollutants affect brain function. The integration of multiple technologies can provide a more comprehensive and in-depth understanding of the impact of pollutants on the brain, provide richer and more accurate data support for the study of neurological diseases caused by pollutants, and help to formulate more effective prevention and control strategies.

[0023] When monitoring excretion and renal toxicity, we combine traditional detection methods with microfluidic chip-based kidney organoid model technology to comprehensively monitor the impact of pollutants on the kidneys: On the one hand, because the kidneys are an important target for the excretion and accumulation of pollutants, traditional testing methods are used to detect indicators such as protein, occult blood, and urine sugar in the urine, as well as renal function indicators such as creatinine and urea nitrogen, to assess whether the renal tubules are damaged. At the same time, the expression levels of genes such as Nr4a1 and Casp3 in kidney tissue are tested to determine the toxic damage of pollutants to the kidneys at the genetic level. On the other hand, using microfluidic chip-based kidney organoid model technology, renal tubular epithelial cells and glomerular mesangial cells are cultured in the cell culture chamber of the microfluidic chip to construct organoid structures with physiological functions. Fluids containing pollutants are transported through microchannels to simulate the blood filtration process. Real-time fluorescence imaging technology in the detection area is used to monitor cell viability (such as changes in mitochondrial membrane potential fluorescence), metabolic function (such as changes in the content of metabolites such as urea and creatinine), and changes in gene expression such as Nr4a1 and Casp3. Although traditional detection methods provide macroscopic information on the overall function and genetic level of the kidney, the microfluidic chip kidney organoid model can simulate the physiological processes of the kidney in real time at the cellular and molecular levels, and the detection is more microscopic and timely. The combination of the two can comprehensively evaluate the toxic effects of pollutants on the kidneys from multiple levels and angles, improve the accuracy and timeliness of kidney toxicity detection, and provide stronger support for the early detection of kidney damage and the formulation of targeted prevention and treatment strategies.

[0024] Step 3: Conduct an interdisciplinary comprehensive analysis and assessment, forming a multidisciplinary research team composed of professionals in environmental science, medicine, and molecular biology to analyze damage to key organs and comprehensively assess the impact of pollutants on human health; Environmental scientists are responsible for monitoring and controlling the characteristics of pollutants in the exposure environment, collecting data on pollutant concentrations, exposure time, and meteorological conditions. Medical personnel conduct clinical indicator testing, disease diagnosis, and imaging analysis, providing relevant data. Molecular biologists conduct research such as gene expression analysis, providing multi-omics data such as genomics, transcriptomics, proteomics, and metabolomics. Multidisciplinary personnel work together to integrate this data to analyze the transmission pathways of pollutants in the body, the mechanisms of damage to various organs, and the inherent connections between organ damage. They clarify the dynamic transmission pathways of pollutants from the respiratory system, through the circulatory system to the liver, spleen, and brain, and ultimately excreted through the urinary system, as well as their systemic toxicity to multiple organs. Simultaneously, using systems biology methods, bioinformatics tools, and network analysis algorithms, they construct a network model of the interaction between airborne pollutants and human health, encompassing molecular nodes such as genes, proteins, and metabolites, and their interactions. By analyzing the network's topological structure, key nodes, and pathways, they comprehensively analyze the molecular mechanisms and network regulatory relationships of pollutants' effects on human health. For example, they identify key genes that play a core role in multiple biological processes and signaling pathways, and the mechanisms by which pollutants affect these genes and thus affect the functions of multiple organs. A comprehensive analysis from macro to micro, from phenomenon to essence has been achieved. On the one hand, multidisciplinary personnel divided the work and cooperated to collect multi-source data, comprehensively covering environmental exposure, clinical manifestations and molecular level information, providing rich materials for research. On the other hand, the network model constructed by the systems biology method can deeply reveal the molecular mechanism and network regulation relationship, and closely link the pollutant transmission path, organ damage and molecular changes. The combination of the two provides a more comprehensive and in-depth basis for the comprehensive evaluation of the comprehensive impact of airborne pollutants on human health, greatly improving the scientific nature and effectiveness of formulating targeted prevention and control measures.

[0025] The above-described embodiments merely illustrate several implementations of the present invention, and while their descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the present invention. It should be noted that a person skilled in the art would be able to make numerous variations and improvements without departing from the spirit of the present invention, all of which fall within the scope of protection of the present invention. Therefore, the scope of protection of the present invention shall be determined by the appended claims.

Claims

1. A method for assessing the effects of airborne pollutants on human health, comprising the following steps: S1. Simulate real exposure environment and build airborne pollutant exposure simulation system; S2. Systematically monitor multiple organ indicators and pollutants; S3. Conduct interdisciplinary comprehensive analysis and assessment, forming a research team composed of multidisciplinary professionals in environmental science, medicine, and molecular biology to analyze damage to key organs and comprehensively assess the impact of pollutants on human health.

2. The method for evaluating the effects of airborne pollutants on human health according to claim 1, wherein: The simulation system in S1 utilizes an airborne nanoplastic exposure device, a nanoplastic aerosol generator, a nanoplastic exposure chamber, an air pump, and an exhaust gas collection device to construct a pollutant exposure scenario close to the real atmospheric environment. The experimental subjects are placed in the nanoplastic exposure chamber for exposure simulation, overcoming the problem that traditional intravenous injection and nasal drip methods cannot accurately simulate the exposure of pollutants in the real atmospheric environment, resulting in insufficient ecological relevance.

3. The method for evaluating the effects of airborne pollutants on human health according to claim 1, wherein: The multiple organs in S2 include the heart, lungs, liver, spleen, brain, and kidneys. The systematic monitoring of pollutants is divided into the entry stage, transmission stage, excretion stage, and renal toxicity stage.

4. The method for evaluating the effects of airborne pollutants on human health according to claim 3, wherein: The monitoring during the entry phase requires exposing the experimental subjects to the airborne pollutant exposure simulation device in S1, and closely monitoring respiratory-related indicators through respiratory monitoring equipment to evaluate the initial impact of pollutants on the respiratory tract and clarify the situation of pollutants entering the body from the respiratory system.

5. The method for evaluating the effects of airborne pollutants on human health according to claim 3, wherein: The monitoring during the transmission stage is to monitor the organs that are preferentially affected after the pollutants enter the body. These organs include the liver, spleen, and brain. The liver, spleen, and brain are selected as the research focus because these organs play a key role in metabolism, immunity, and neural function, and can comprehensively reflect the toxicity mechanism of pollutants.

6. The method for evaluating the effects of airborne pollutants on human health according to claim 3, wherein: The excretion and renal toxicity stage monitoring is because pollutants are eventually excreted from the body through the urinary system. The kidneys, as the main excretory organ, are responsible for filtering harmful substances in the blood, and therefore become another important target for pollutant accumulation and toxic effects.

7. The method for evaluating the effects of airborne pollutants on human health according to claim 1, wherein: The multidisciplinary personnel in S3 jointly integrate data, analyze the transmission paths of pollutants in the body, the damage mechanisms to various organs, and the intrinsic connections between the damage to various organs, comprehensively evaluate the comprehensive impact of airborne pollutants on human health, and provide a basis for formulating targeted prevention and control measures.

8. The method for evaluating the effects of airborne pollutants on human health according to claim 1, wherein: The S1 uses a laser scattering particle size analyzer to monitor the nanoplastic aerosol particle size in real time, and automatically adjusts the nanoplastic aerosol generator parameters through a feedback control system to stabilize the nanoplastic aerosol particle size within the target range of ±10nm.

9. The method for evaluating the effects of airborne pollutants on human health according to claim 1, wherein: During the S2 stage monitoring, a nanotechnology-based respiratory sensor array is used to detect the concentrations of five different types of airborne pollutants, with a detection limit as low as 0.1 μg / m³, to more accurately assess the entry of pollutants into the body; when monitoring the liver during the transmission stage, a liver cell gene knockout or overexpression model is constructed through gene editing technology to study the mechanism of action of specific genes in the effects of pollutants on liver metabolism and detoxification function; when monitoring the spleen, proteomics and phosphoproteomics technologies are combined to comprehensively analyze the changes in protein expression and phosphorylation modification of splenic immune cells under the action of pollutants, and identify key proteins and modification sites related to abnormal immune regulation; when monitoring the brain, optogenetics technology is used to specifically regulate the activity of specific neurons in the brain, and combined with in vivo multi-electrode array recording technology, the effects of pollutant exposure on neuronal electrical activity and neural circuit function are studied; during the excretion and renal toxicity stage monitoring, a kidney organoid model based on a microfluidic chip is used to simulate the physiological function of the kidney, and the toxic effects of pollutants on kidney cells are monitored in real time on the chip.

10. The method for evaluating the effects of airborne pollutants on human health according to claim 1, wherein: In the S3 interdisciplinary comprehensive analysis and evaluation, systems biology methods were used to construct a network model of the interaction between airborne pollutants and human health, integrating multi-omics data to comprehensively analyze the molecular mechanisms and network regulatory relationships of the impact of pollutants on human health.

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