Method for controlling plant pathogenic fungi

Abstract

The present disclosure relates to methods of controlling fungal diseases in soybean crops. The present disclosure also relates to fungicidal combinations for controlling fungal diseases in soybean crops. The present disclosure also relates to corresponding compositions and uses thereof.

Description

Technical Field

[0001] The present invention relates to a method for controlling fungal diseases in soybean crops. The present invention also relates to a fungicidal combination for controlling fungal diseases in soybean crops.

[0002] Background and prior art

[0003] Soybean (Glycine max (L.) Merr.) is the major commercial oilseed crop produced and consumed worldwide. It is arguably the most widely used row crop on Earth. It has been cultivated for millennia in temperate ecosystems. Originally cultivated in northern Asia, it has been grown in North America and Latin America in recent decades. It is estimated that soybeans are cultivated on 6% of the world's arable land, and since the 1970s, soybeans have experienced the largest percentage increase in cultivated area compared to any other major crop. Currently, soybeans account for approximately 55% of global oilseed production, and over the past decade, soybean production has grown at an average annual rate of over 5%.

[0004] Soybean crops are always susceptible to bacterial, fungal, or viral diseases. Potential yield losses associated with disease infection can range from minimal to excessively devastating, depending on the disease, environment, timing of infection, and soybean product. Soybean leaves are susceptible to attack by a number of fungal and bacterial pathogens. These pathogens can cause leaf spots and wilts.

[0005] Important fungal diseases that cause significant economic losses to soybean cultivation include Asian soybean rust (ASR) caused by Phakopsora pachyrhizi, powdery mildew caused by Erysiphe diffusa, gray leaf spot (FLS) caused by the fungus Cercospora sojina, brown leaf spot caused by Septoria glycines, and several other diseases.

[0006] Powdery mildew, caused by the fungus Erysiphe disseminata (synonymous with soybean powdery fungus, Microsphaera diffusa), is a significant disease of soybean cultivation, particularly common in the United States, Paraguay, Brazil, and Bolivia. The most effective and economical method for controlling powdery mildew is the use of resistant soybean cultivars. Yield losses of up to 50%-60% due to powdery mildew infection have been reported in many countries. However, due to the high variability in the pathogenicity of Erysiphe disseminata, resistance in some cultivars may be overcome by the pathogen. Furthermore, no resistant soybean cultivars are currently available.

[0007] Soybean rust is a devastating foliar disease of soybeans caused by Phakopsora pachyrhizi. Asian soybean rust is an aggressive disease that damages photosynthetic tissue, leading to premature defoliation, premature maturity, and lower yields.

[0008] The massive losses caused by the continued influx of fungal pests have long-term consequences for crop production, quality, and yield. Chemical control is the most widely used method. However, indiscriminate use of these products can lead to increased toxicity, residues in the environment, and the selection of resistant strains of pathogens.

[0009] Therefore, there is a need in the art to provide alternative fungicidal combinations and methods for effectively controlling phytopathogenic fungi that cause diseases in soybean cultivation.

[0010] Purpose of the Invention

[0011] An object of the present invention is to provide a fungicidal combination for controlling phytopathogenic fungi in soybean crops.

[0012] Another object of the present invention is to provide a method for controlling phytopathogenic fungi in soybean crops.

[0013] Another object of the present invention is to provide a fungicidal composition for controlling phytopathogenic fungi in soybean crops.

[0014] Another object of the present invention is to provide a use of a fungicidal combination for controlling phytopathogenic fungi in soybean crops. Summary of the Invention

[0015] The present invention provides a fungicide combination for controlling plant pathogenic fungi in soybean crops.

[0016] In one aspect, the present invention provides a fungicidal combination for controlling phytopathogenic fungi in soybean crops, the fungicidal combination comprising one or more multi-site fungicides.

[0017] In one aspect, the present invention provides a fungicidal combination for controlling phytopathogenic fungi in soybean crops, the fungicidal combination comprising at least two multi-site fungicides.

[0018] In another aspect, the present invention provides a fungicidal combination for controlling phytopathogenic fungi in soybean crops, the fungicidal combination comprising at least two multi-site fungicides selected from copper or a salt thereof and sulfur.

[0019] In another aspect, the present invention provides a fungicidal combination for controlling phytopathogenic fungi in soybean crops, the fungicidal combination comprising at least two multi-site fungicides selected from copper or its salts and sulfur and one or more systemic fungicides.

[0020] The present invention provides a method for controlling fungal diseases in soybean crops.

[0021] In one aspect, the present invention provides a method of controlling fungal diseases comprising applying one or more multi-site fungicides to plants, plant parts, loci, or propagation material in a soybean crop.

[0022] In another aspect, the present invention provides a method of controlling fungal diseases comprising applying at least two multi-site fungicides selected from copper or a salt thereof and sulfur to plants, plant parts, loci or propagation material in a soybean crop.

[0023] In another aspect, the present invention provides a method of controlling fungal diseases comprising applying at least two multi-site fungicides selected from copper or its salts and sulfur and one or more systemic fungicides to plants, plant parts, loci or propagation material in a soybean crop.

[0024] In another aspect, the present invention provides a fungicidal composition comprising:

[0025] (i) at least two multi-site fungicides selected from copper or its salts and sulfur, and

[0026] (ii) at least one agrochemically acceptable excipient.

[0027] In another aspect, the present invention provides a fungicidal composition comprising:

[0028] (i) at least two multi-site fungicides selected from copper or its salts and sulfur,

[0029] (ii) one or more systemic fungicides, and

[0030] (iii) at least one agrochemically acceptable excipient.

[0031] In yet another aspect, the present invention provides use of a fungicidal combination comprising at least two multi-site fungicides selected from copper or a salt thereof and sulfur for controlling phytopathogenic fungi in soybean crops.

[0032] In yet another aspect, the present invention provides use of a fungicidal combination comprising at least two multi-site fungicides selected from copper or its salts and sulfur and at least two systemic fungicides for controlling phytopathogenic fungi in soybean crops.

[0033] In another aspect, the present disclosure provides a kit comprising a plurality of components, including at least one of the components of the fungicidal combination of the present disclosure. Detailed Description of the Invention

[0035] For purposes of the following detailed description, it is to be understood that the invention may assume various alternative variations, except where expressly specified to the contrary.

[0036] Furthermore, except in any operating examples or where otherwise indicated, all numbers expressing, for example, amounts of materials / ingredients used in the specification are to be understood in all instances as modified by the term "about." The term "about," as used to qualify the amount of an active ingredient, is to be interpreted as meaning "approximately" or "quite approximately," and any statistically insignificant variation therefrom.

[0037] As used herein, the terms "comprising," "including," "having," "containing," "involving," etc. should be understood as open-ended, meaning including but not limited to.

[0038] In any aspect or embodiment described below, the phrase "comprising" may be replaced with the phrase "consisting of" or "consisting essentially of. In these aspects or embodiments, the combinations or compositions described include or comprise or consist of or consist essentially of or consist essentially of the specific components recited therein, so as to exclude other ingredients or excipients not specifically recited therein.

[0039] The phrase "fungicidally effective amount" refers to an amount of a fungicide that kills or inhibits the desired phytopathogenic disease, but which is not significantly toxic to the plant being treated.

[0040] As used herein, the term "disease control" refers to the control and prevention of diseases. The control effect includes all deviations from natural development, for example: killing fungal diseases, delaying fungal diseases, inhibiting fungal diseases, or reducing fungal diseases.

[0041] The term "plant" refers to all physical parts of a plant, including seeds, seedlings, saplings, roots, tubers, stalks, stems, leaves and fruit.

[0042] As used herein, the term "locus" of a plant is intended to include the place where the plant is growing, where plant propagation material of the plant is sown or placed in the soil.

[0043] The term "plant propagation material" is understood to mean the reproductive parts of an indicated plant, such as seeds, vegetable material such as cuttings or tubers, roots, fruits, tubers, bulbs, rhizomes and parts of plants, sprouted plants and young plants to be transplanted after germination or after breaking the soil. These young plants may be protected by being immersed in whole or in part before transplanting.

[0044] The term "agriculturally acceptable amount of active substance" refers to an amount of active substance that kills or inhibits the plant disease to be controlled, which amount is not significantly toxic to the plants to be treated.

[0045] As used herein, the term % disease severity refers to the percentage of rot observed in a crop, expressed as the percentage of relevant host tissue in the crop that is covered by lesions or damaged by the disease. Severity depends on the number and size of lesions. % severity indicates the extent of damage caused by the disease.

[0046] As used herein, the term % disease control refers to the % control and prevention of diseases in crops.

[0047] The term "g ai / L" as used herein means the concentration of the respective active ingredient present in "grams" per "liter" of a composition or combination.

[0048] As used herein, the term "g ai / h" means the concentration of the corresponding active ingredient applied in "grams" per "hectare" of agricultural land.

[0049] Each of the above aspects can have one or more embodiments.

[0050] Each embodiment in the embodiment described below may be applicable to one or all aspects in the aspect described above. These embodiments are intended to be understood as preferred features of one or all aspects described above. Each embodiment in the embodiment described below is applicable to each aspect in the aspect described above individually.

[0051] Through the present disclosure, the inventors have successfully developed a fungicide combination that effectively inhibits the growth of fungal plant pathogens that cause fungal diseases observed in soybean cultivation, such as Asian soybean rust (ASR) caused by Phakopsora pachyrhizi, powdery mildew caused by Erysiphe spp., gray leaf spot (FLS) caused by Cercospora sojae, and brown leaf spot caused by Septoria sojae. A variety of plant pathogens are known to cause diseases in soybean cultivation. The inventors have demonstrated significant inventive ingenuity in developing the fungicide combination of the present invention, comprising at least two multi-site fungicides, to achieve inhibition of fungal plant pathogens, particularly P. pachyrhizi, which causes Asian soybean rust (ASR) in soybeans, Erysiphe spp., which causes powdery mildew, as well as other fungal plant pathogens that infect soybean cultivation. Thus, the inventors provide a fungicide combination comprising a copper fungicide and sulfur to exert inhibitory activity against fungal plant pathogens in soybean crops.

[0052] In one embodiment, the present invention provides a fungicidal combination for controlling phytopathogenic fungi in soybean crops, the fungicidal combination comprising one or more multi-site fungicides.

[0053] In one embodiment, the present invention provides a fungicidal combination comprising at least two multi-site fungicides for controlling plant pathogenic fungi - Phakopsora pachyrhizi, which causes Asian soybean rust (ASR), Erysiphe sphaeroides, which causes powdery mildew, Cercospora sojae, which causes gray leaf spot (FLS), and Septoria sojae, which causes brown leaf spot in soybean crops.

[0054] In one embodiment, the multi-site fungicide is selected from copper or its salts, sulfur, dithiocarbamates, phthalimides, chloronitriles, sulfonamides, biguanides, triazines, quinones, quinoxalines, maleimides, and thiocarbamates.

[0055] In a preferred embodiment, the multi-site fungicide comprises copper or a salt thereof.

[0056] In one embodiment, the copper compound is a copper salt.

[0057] In one embodiment, the copper compound is a copper salt selected from copper sulfate, tribasic copper sulfate, dibasic copper sulfate, copper oxychloride, copper chloride, copper oxide, copper oxide, copper nitrate, copper perchlorate, copper bromide, copper hydroxide, copper iodide, copper acetate, or copper sulfate pentahydrate.

[0058] In a preferred embodiment, the copper compound is tribasic copper sulfate.

[0059] In one embodiment, the copper compound is copper sulfate.

[0060] In a preferred embodiment, the multi-site fungicide is sulfur.

[0061] In one embodiment, the sulfur is of particle size distribution based on a diameter D of <100 microns. 90 of granular sulfur.

[0062] In one embodiment, the sulfur is of particle size distribution based on a diameter D of <50 microns. 90 of granular sulfur.

[0063] In one embodiment, the sulfur is of particle size distribution based on a diameter D of <25 microns. 90 of granular sulfur.

[0064] In one embodiment, the sulfur is of particle size distribution based on a diameter D of about 1 micron to 3 microns. 90 The particle size distribution is based on a diameter D of about 1 micron to 3 microns. 90 The suspension concentrates, used interchangeably alone or in combination with a second multi-site contact fungicide, all demonstrated significantly improved efficacy against fungal infections.

[0065] The particle size distribution of the particulate sulfur component of the present invention has a D of about 1.2 microns. 90 .

[0066] In one embodiment, one of the multi-site fungicides of the present invention may be a dithiocarbamate fungicide selected from the group consisting of mancozeb, ferbam, mancozeb, maneb, metiram, propineb, thiram, zinc thiazole, zineb, and ziram.

[0067] In one embodiment, one of the multi-site fungicides of the present invention may be a phthalimide fungicide selected from the group consisting of captan, captafol, and folpet.

[0068] In one embodiment, one of the multi-site fungicides of the present invention may be thiophanate-methyl.

[0069] In one embodiment, one of the multi-site fungicides of the present invention may be a sulfonamide fungicide selected from diflubenzuron and tolylfluanid.

[0070] In one embodiment, one of the multi-site fungicides of the present invention may be a biguanide fungicide selected from the group consisting of octylcarbamate and octylcarbamate.

[0071] In one embodiment, one of the multi-site fungicides of the present invention may be a triazine fungicide selected from difop-butyl.

[0072] In one embodiment, one of the multi-site fungicides of the present invention may be a quinone fungicide selected from dithianon.

[0073] In one embodiment, one of the multi-site fungicides of the present invention may be a quinoxaline fungicide selected from chinomethionats.

[0074] In one embodiment, one of the multi-site fungicides of the present invention may be a maleimide fungicide selected from thiazolinone.

[0075] In one embodiment, one of the multi-site fungicides of the present invention may be a thiocarbamate fungicide selected from sulfamethoxam.

[0076] In a preferred embodiment, the present invention provides a fungicidal combination for controlling fungal infestations in soybean crops, the combination comprising one or more multi-site fungicides selected from copper salts and sulfur.

[0077] In a preferred embodiment, the present invention provides a fungicidal combination for controlling Phakopsora pachyrhizi that causes Asian soybean rust (ASR) in soybeans, the combination comprising one or more multi-site fungicides selected from copper salts and sulfur.

[0078] In a preferred embodiment, the present invention provides a fungicidal combination for controlling Erysiphe disseminated mildew that causes powdery mildew in soybeans, the combination comprising one or more multi-site fungicides selected from copper salts and sulfur.

[0079] It should be noted that the order of the fungicides in the combination does not have to be the same as described in the preferred embodiments above and are interchangeable.

[0080] In one embodiment, the present invention provides a fungicidal combination for controlling Phakopsora pachyrhizi that causes Asian soybean rust (ASR) in soybeans, the fungicidal combination comprising at least two multi-site fungicides selected from copper or its salts and sulfur.

[0081] In one embodiment, the present invention provides a fungicidal combination for controlling Erysiphe disseminated mildew that causes powdery mildew in soybeans, the fungicidal combination comprising at least two multi-site fungicides selected from copper or a salt thereof and sulfur.

[0082] In one embodiment, the present invention provides a fungicidal combination for controlling phytopathogenic fungal infestations in soybeans, the fungicidal combination comprising at least two multi-site fungicides, wherein the first multi-site fungicide is tribasic copper sulfate and the second multi-site fungicide is sulfur.

[0083] In one embodiment, the present invention provides a fungicidal combination for controlling Phakopsora pachyrhizi that causes Asian soybean rust (ASR) in soybeans, the fungicidal combination comprising at least two multi-site fungicides, wherein the first multi-site fungicide is tribasic copper sulfate and the second multi-site fungicide is sulfur.

[0084] In one embodiment, the present invention provides a fungicidal combination for controlling Erysiphe disseminated mildew that causes powdery mildew in soybeans, the fungicidal combination comprising at least two multi-site fungicides, wherein the first multi-site fungicide is tribasic copper sulfate and the second multi-site fungicide is sulfur.

[0085] In one embodiment, the present invention provides a fungicidal combination for controlling fungal infestation in soybeans, the fungicidal combination comprising at least two multi-site fungicides selected from copper or its salts and dithiocarbamate fungicides.

[0086] In one embodiment, the present invention provides a fungicidal combination for controlling Phakopsora pachyrhizi that causes Asian soybean rust (ASR) in soybean, the fungicidal combination comprising at least two multi-site fungicides selected from tribasic copper sulfate and mancozeb.

[0087] In one embodiment, the present invention provides a fungicidal combination for controlling Erysiphe disseminated mildew that causes powdery mildew in soybeans, the fungicidal combination comprising at least two multi-site fungicides selected from tribasic copper sulfate and mancozeb.

[0088] In one embodiment, the present invention provides a fungicidal combination for controlling plant pathogenic fungi in soybean cultivation, the fungicidal combination comprising at least two multi-site fungicides selected from sulfur and dithiocarbamates.

[0089] In one embodiment, the present invention provides a fungicidal combination for controlling Phakopsora pachyrhizi that causes Asian soybean rust (ASR) in soybean crops, the fungicidal combination comprising at least two multi-site fungicides selected from sulfur and mancozeb.

[0090] In one embodiment, the present invention provides a fungicidal combination for controlling Erysiphe graminis causing powdery mildew in soybean crops, the fungicidal combination comprising at least two multi-site fungicides selected from sulfur and mancozeb.

[0091] In one embodiment, the present invention provides a fungicidal combination for controlling plant pathogenic fungi in soybean cultivation, the combination comprising a first multi-site fungicide selected from copper salts and a second multi-site fungicidal compound selected from sulfur, wherein the weight ratio of copper salt to sulfur ranges from 1:15 to 15:1.

[0092] In one embodiment, the present invention provides a fungicidal combination for controlling plant pathogenic fungi in soybean cultivation, the combination comprising a first multi-site fungicide selected from copper salts and a second multi-site fungicidal compound selected from sulfur, wherein the weight ratio of copper salt to sulfur ranges from 1:1 to 1:15.

[0093] In one embodiment, the present invention provides a fungicidal combination for controlling plant pathogenic fungi in soybean cultivation, the combination comprising a first multi-site fungicide selected from copper salts and a second multi-site fungicidal compound selected from sulfur, wherein the weight ratio of copper salt to sulfur ranges from 1:1 to 1:10.

[0094] In one embodiment, the present invention provides a fungicidal combination for controlling plant pathogenic fungi in soybean cultivation, the combination comprising a first multi-site fungicide selected from copper salts and a second multi-site fungicidal compound selected from sulfur, wherein the weight ratio of copper salt to sulfur is 1:8.

[0095] In one embodiment, the present invention provides a fungicidal combination for controlling plant pathogenic fungi in soybean cultivation, the combination comprising tribasic copper sulfate and a sulfur compound, wherein the weight ratio of tribasic copper sulfate to sulfur ranges from 1:15 to 15:1.

[0096] In one embodiment, the present invention provides a fungicidal combination for controlling plant pathogenic fungi in soybean cultivation, the combination comprising tribasic copper sulfate and a sulfur compound, wherein the weight ratio of tribasic copper sulfate to sulfur ranges from 1:1 to 1:15.

[0097] In one embodiment, the present invention provides a fungicidal combination for controlling plant pathogenic fungi in soybean cultivation, the combination comprising tribasic copper sulfate and a sulfur compound, wherein the weight ratio of tribasic copper sulfate to sulfur ranges from 1:1 to 1:10.

[0098] In one embodiment, the present invention provides a fungicidal combination for controlling plant pathogenic fungi in soybean cultivation, the combination comprising tribasic copper sulfate and a sulfur compound, wherein the weight ratio of tribasic copper sulfate to sulfur is in the range of 1:8.

[0099] In a preferred embodiment, the present invention provides a fungicidal combination for controlling Phakopsora pachyrhizi that causes Asian soybean rust (ASR) in soybean, the combination comprising a first multi-site fungicide selected from copper salts and a second multi-site fungicidal compound selected from sulfur, wherein the weight ratio of copper or its salt to sulfur ranges from 1:1 to 1:15.

[0100] In a preferred embodiment, the present invention provides a fungicidal combination for controlling Phakopsora pachyrhizi that causes Asian soybean rust (ASR) in soybean, the combination comprising a first multi-site fungicide selected from copper salts and a second multi-site fungicidal compound selected from sulfur, wherein the weight ratio of copper or its salt to sulfur ranges from 1:1 to 1:10.

[0101] In a more preferred embodiment, the present invention provides a fungicidal combination for controlling Phakopsora pachyrhizi that causes Asian soybean rust (ASR) in soybean cultivation, the combination comprising a first multi-site fungicide selected from copper salts and a second multi-site fungicidal compound selected from sulfur, wherein the weight ratio of copper or its salt to sulfur is 1:8.

[0102] In a preferred embodiment, the present invention provides a fungicidal combination for controlling Erysiphe graminis causing powdery mildew in soybean crops, the combination comprising a first multi-site fungicide selected from copper salts and a second multi-site fungicidal compound selected from sulfur, wherein the weight ratio of copper or its salt to sulfur ranges from 1:1 to 1:15.

[0103] In a preferred embodiment, the present invention provides a fungicidal combination for controlling Erysiphe graminis causing powdery mildew in soybean crops, the combination comprising a first multi-site fungicide selected from copper salts and a second multi-site fungicidal compound selected from sulfur, wherein the weight ratio of copper or its salt to sulfur ranges from 1:1 to 1:10.

[0104] In a more preferred embodiment, the present invention provides a fungicidal combination for controlling powdery mildew causing powdery mildew in soybean cultivation, the combination comprising a first multi-site fungicide selected from copper salts and a second multi-site fungicidal compound selected from sulfur, wherein the weight ratio of copper or its salt to sulfur is 1:8.

[0105] In one embodiment, the present invention provides a fungicidal combination for controlling Phakopsora pachyrhizi, which causes Asian soybean rust (ASR) in soybeans, and Erysiphe graminis, which causes powdery mildew, in soybeans, the combination comprising a first multi-site fungicide selected from copper salts and a second multi-site fungicidal compound selected from dithiocarbamate fungicides, wherein the weight ratio of copper salt to dithiocarbamate fungicide ranges from 1:1 to 1:15.

[0106] In one embodiment, the present invention provides a fungicidal combination for controlling Phakopsora pachyrhizi, which causes Asian soybean rust (ASR), and Erysiphe graminis, which causes powdery mildew in soybean cultivation, the combination comprising a first multi-site fungicide selected from copper salts and a second multi-site fungicidal compound selected from dithiocarbamate fungicides, wherein the weight ratio of copper salt to dithiocarbamate fungicide ranges from 1:1 to 1:10.

[0107] In one embodiment, the present invention provides a fungicidal combination for controlling Phakopsora pachyrhizi that causes Asian soybean rust (ASR) in soybean and Erysiphe graminis that causes powdery mildew, the combination comprising tribasic copper sulfate and mancozeb, wherein the weight ratio of tribasic copper sulfate to mancozeb ranges from 1:5 to 1:10.

[0108] In one embodiment, the present invention provides a fungicidal combination for controlling Phakopsora pachyrhizi, which causes Asian soybean rust (ASR), and Erysiphe graminis, which causes powdery mildew in soybean cultivation, the combination comprising tribasic copper sulfate and mancozeb, wherein the weight ratio of tribasic copper sulfate to mancozeb is 1:8.

[0109] In one embodiment, the present invention provides a fungicidal combination for controlling phytopathogenic fungi in soybean cultivation, the fungicidal combination comprising at least two multi-site fungicides and at least one additional fungicide.

[0110] Soybean fungal diseases encompassed within the scope of the present invention include Phakopsora pachyrhizi, which causes Asian soybean rust (ASR); Erysiphe disseminated, which causes powdery mildew; Cercospora sojae, which causes gray spot (FLS); Septoria sojae, which causes brown spot, Phytophthora root rot, stem and pod blight, brown spot, downy mildew, Cercopsora leaf blight, and purple spot; and Sclerotinia stem rot (Sclerotinia).

[0111] In another embodiment, the present invention provides a fungicidal combination comprising at least two multi-site fungicides and at least one additional fungicide selected from the group consisting of morpholine fungicides, triazole fungicides, acylamino acid fungicides, aniline fungicides, antibiotic fungicides, strobilurin fungicides, aromatic fungicides, arsenic fungicides, arylphenyl ketone fungicides, benzimidazole fungicides, benzimidazole precursor fungicides, benzothiazole fungicides, bridged diphenyl fungicides, carbamate fungicides, conazole fungicides, copper fungicides, dicarboximide fungicides, dinitrile fungicides, phenol fungicides, dithiocarbamate fungicides, dithiolane fungicides, hydrazide fungicides, imidazole fungicides, inorganic fungicides, organophosphorus fungicides, organotin fungicides, oxathiin fungicides, oxazole fungicides, pyrimidine fungicides, pyrrole fungicides, quinoline fungicides, quinone fungicides, quinoxaline fungicides, thiadiazole fungicides, thiazole fungicides, thiazolidine fungicides, thiocarbamate fungicides, thiophene fungicides, triazine fungicides, triazolopyrimidine fungicides, urea fungicides, zinc fungicides, unclassified fungicides and mixtures thereof.

[0112] In one embodiment, the additional fungicide is a systemic fungicide.As used herein, the term "systemic fungicide" refers to a fungicide that is taken up by leaves and roots and translocated upwards into the interior of the plant through the xylem.

[0113] In one embodiment, the present invention provides a fungicide combination comprising at least two multi-site fungicides and at least one systemic fungicide for controlling plant pathogenic fungi - Phakopsora pachyrhizi, which causes Asian soybean rust (ASR), and Erysiphe graminis, which causes powdery mildew, in soybean cultivation.

[0114] In one embodiment, the present invention provides a fungicide combination comprising at least two multi-site fungicides and at least two systemic fungicides for controlling plant pathogenic fungi - Psora pachyrhizi, which causes Asian soybean rust (ASR) in soybean cultivation; Psora disseminated powdery mildew; Cercospora sojae, which causes gray leaf spot (FLS); Septoria sojae, which causes brown spot, Phytophthora root rot, stem and pod blight, brown spot, downy mildew, Cercospora leaf blight and purple spot, and Sclerotinia stem rot (sclerotinia) in soybean cultivation.

[0115] In one embodiment, the systemic fungicides particularly suitable for use in the combination according to the invention belong to the following group: acylalanines (metalaxyl, metalaxyl-M), benzimidazoles (benomyl, carbendazim, thiabendazole or thiophanate-methyl), oxathioxines (carboxin or oxycarboxin), organophosphates (aluminum phosphoethyl), pyrimidines (dimethoxythiazolin, pyrimoxydim, pyrimidinesulfonate, clofenac, cyprodinil or flufenac), triazoles (conazoles or imidazoles - such as triadimefon, bifenac, difenoconazole, propiconazole, myclobutanil, cyproconazole, prochloraz, furfural,

[0014] The present invention also includes but is not limited to: fungicides such as oxaclostrobin, oxiconazole, metconazole, or tebuconazole), strobilurins (QoI fungicides, azoxystrobin, trifloxystrobin, pyraclostrobin, kresoxim-methyl, or kresoxim-methyl), triazolinethiones (prothioconazole), succinate dehydrogenase inhibitor (SDHI) fungicides (benzovinfluazide, bixafen, boscalid, carboxin, fluopyram, fluopyram, pyraclostrobin, penthiopyrad, fluopyram), piperidines (fenpropidin), organochlorines (chlorothalonil), morpholine analog fungicides (fenpropimorph, fenpropidin, or amorolfine), sulfur fungicides, or any combination thereof.

[0116] In one embodiment, the present invention provides a fungicidal combination for controlling plant pathogenic fungi in soybean cultivation, the fungicidal combination comprising at least two multi-site fungicides and at least one systemic fungicide.

[0117] In one embodiment, the present invention provides a fungicidal combination for controlling plant pathogenic fungi in soybean cultivation, the fungicidal combination comprising tribasic copper sulfate, sulfur and metalaxyl or an isomer thereof.

[0118] In one embodiment, the present invention provides a fungicidal combination for controlling plant pathogenic fungi in soybean cultivation, the fungicidal combination comprising tribasic copper sulfate, sulfur and carboxin.

[0119] In one embodiment, the present invention provides a fungicidal combination for controlling plant pathogenic fungi in soybean cultivation, the fungicidal combination comprising tribasic copper sulfate, sulfur and tebuconazole.

[0120] In one embodiment, the present invention provides a fungicidal combination for controlling plant pathogenic fungi in soybean cultivation, the fungicidal combination comprising tribasic copper sulfate, sulfur and difenoconazole.

[0121] In one embodiment, the present invention provides a fungicidal combination for controlling plant pathogenic fungi in soybean cultivation, the fungicidal combination comprising tribasic copper sulfate, sulfur and prothioconazole.

[0122] In one embodiment, the present invention provides a fungicidal combination for controlling plant pathogenic fungi in soybean cultivation, the fungicidal combination comprising tribasic copper sulfate, sulfur and azoxystrobin.

[0123] In one embodiment, the present invention provides a fungicidal combination for controlling plant pathogenic fungi in soybean crops, the fungicidal combination comprising tribasic copper sulfate, sulfur and trifloxystrobin.

[0124] The combination of the present invention may be provided as a premix composition or a kit-of-parts so that the separate active substances can be mixed before spraying. Alternatively, the kit-of-parts may contain at least two multi-site fungicides comprising a premixed combination of copper or a salt thereof and sulfur and an optional third active ingredient which may be mixed with an adjuvant so that the two components can be tank-mixed before spraying.

[0125] Thus, in one embodiment, the present invention provides a kit comprising a fungicidal combination comprising sulfur and tribasic copper sulfate.

[0126] In another embodiment, the present invention provides a kit comprising a fungicidal combination comprising sulfur and tribasic copper sulfate; and optionally a third fungicide selected from the group consisting of morpholine fungicides, triazole fungicides, acylamino acid fungicides, aniline fungicides, antibiotic fungicides, strobilurin fungicides, aromatic fungicides, arsenic fungicides, arylphenyl ketone fungicides, benzimidazole fungicides, benzimidazole precursor fungicides, benzothiazole fungicides, bridged diphenyl fungicides, carbamate fungicides, conazole fungicides, copper fungicides, dicarboximide fungicides, dioxadiazole fungicides, thiazolinone ... Nitrophenol fungicides, dithiocarbamate fungicides, dithiolane fungicides, hydrazide fungicides, imidazole fungicides, inorganic fungicides, organophosphorus fungicides, organotin fungicides, oxathiin fungicides, oxazole fungicides, pyrimidine fungicides, pyrrole fungicides, quinoline fungicides, quinone fungicides, quinoxaline fungicides, thiadiazole fungicides, thiazole fungicides, thiazolidine fungicides, thiocarbamate fungicides, thiophene fungicides, triazine fungicides, triazolopyrimidine fungicides, urea fungicides, zinc fungicides, unclassified fungicides and mixtures thereof.

[0127] The combination of the present disclosure may be sold as a premix composition or a kit of parts so that the individual active substances can be mixed before spraying. Alternatively, the kit of parts may contain at least two multi-site fungicides premixed, and the third active substance may be mixed with an adjuvant so that the two components can be tank mixed before spraying.

[0128] In one embodiment, the systemic fungicide may be a single fungicide or a combination of two or more systemic fungicides.

[0129] In one embodiment, the systemic fungicide is a combination of at least two fungicides.

[0130] In one embodiment, the systemic fungicide in the combination can be selected from nucleic acid synthesis inhibitors, cytoskeleton and motor protein inhibitors, amino acid and protein synthesis inhibitors, respiratory process inhibitors, signal transduction inhibitors, lipid synthesis and membrane integrity disruptors, sterol biosynthesis inhibitors, melanin synthesis inhibitors, cell wall biosynthesis inhibitors, inhibitors of melanin synthesis in the cell wall, host plant defense inducers, fungicides with unknown mode of action, unclassified fungicides or biological agents with multiple modes of action.

[0131] Thus, in one embodiment, the nucleic acid synthesis inhibitor fungicide may be selected from acylalanines such as bensulfuron, bensulfuron-M (bensulfuron-M), furalaxyl, metalaxyl, metalaxyl-M (metalaxyl-M); oxazolidinones such as oxalaxyl; butyrolactones such as furamide; hydroxy-(2-amino-)pyrimidines such as ethiopyrimidine, formidable, and ethiopyrimidine; isoxazoles such as oxamexazole; isothiazolinones such as octhiazol; carboxylic acids such as oxolinic acid.

[0132] In one embodiment, the cytoskeleton and motor protein inhibitors can be benzimidazoles such as benomyl, carbendazim, thiabendazole, thiophanate-methyl; thiophanate-methyl such as thiophanate-methyl, N-phenylcarbamates such as ethiclocarb; toluamides such as benomyl; thiazolecarboxamides such as ethaboxam; phenylureas such as pencuron, benzamides such as fluopyram; cyanoacrylates such as cyproconazole.

[0133] In one embodiment, the respiratory process inhibitor fungicide may be selected from the group consisting of pyrimidines such as diflunisal; pyrazole-5-carboxamides such as tolfenpyrad; strobilurins such as azoxystrobin, syringystrobin, enoxastrobin, flutoxifen, picoxystrobin, pyraclostrobin, mandestrobin, pyraclostrobin, pyraclostrobin, chlorpyrifos-methyl, kresoxim-methyl, kresoxim-methyl, enoxastrobin, fenoxam, trifloxystrobin, famoxadone, fluoxastrobin, fenamidone, pyraclostrobin and mixtures thereof; oxazolidinones such as famoxadone; imidazoline Ketones such as fenamidone; benzyl-carbamates such as pyraclostrobin; N-methoxy-(phenyl-ethyl)-pyrazole-carboxamides such as pyrimidineamines such as diflunisal; cyanoimidazoles such as cyazofamid; sulfamoyltriazoles such as indazolesulfamide; dinitrophenylcrotonates such as binapacryl, diclofenac, diclofenac; 2,6-dinitroanilines such as fluazinam; pyrrolidonehydrazones such as pyrimidine; triphenyltin compounds such as triphenyltin acetate, triphenyltin chloride, and pyrimidine; thiophene-carboxamides such as silthiopyrad; triazolopyrimidineamines such as pyraclostrobin.

[0134] In one embodiment, the amino acid and protein synthesis inhibitor fungicides may be selected from anilino-pyrimidines such as cyprodinil, myclobutanil, pyrimethanil, antibiotic fungicides such as blasticidin-S, kasugamycin, streptomycin, oxytetracycline, and the like.

[0135] In one embodiment, the signal transduction inhibitor fungicide may be selected from aryloxyquinolines such as quinoxyfen; quinazolinones such as propoxyquin; phenylpyrroles such as fenpiclonil, fludioxonil; imides such as ethoxyconazole, sclerotinol, iprodione, procymidone and vinclozolin.

[0136] In one embodiment, the third fungicide can be selected from lipid synthesis and membrane integrity disruptors such as thiophosphates such as dichlorvos, isothiocyanate, pyraclostrobin; dithiolanes such as pyraclostrobin; aromatic hydrocarbons such as biphenyl, chloranil, chlornitril, pentachloronitrobenzene (PCNB), tetrachloronitrobenzene (TCNB), methyl tolclofos, etc.; 1,2,4-thiadiazoles such as clozolin; carbamates such as iodopropynyl butylcarbamate, propamocarb, thiophanate, etc.

[0137] Thus, in one embodiment, the sterol biosynthesis inhibitor may be selected from triazoles such as azaconazole, bifenthiazolin, oxadiazole, cyproconazole, difenoconazole, diniconazole, epoxiconazole, ethiconazole, fenbuconazole, fluquinconazole, flusilazole, flutriafol, hexaconazole, amide, picronazole, metconazole, myclobutanil, penconazole, propiconazole, silyconazole, tebuconazole, fluconazole, triadimefon, triadimenol, triticonazole, prothioconazole; piperazines such as triazinon; pyridines such as pyrimidine oxime, chloramphenicol; pyraclostrobin; pyrimidines such as chlorfenapyr and flufenapyr; imidazoles such as imazalil, oxaflutazole, pyraclostrobin, prochloraz, triflumizole; morpholines such as aldimorph, morpholino, fenpropimorph, and chloranil; piperidines such as fenpropidin and fenpropimorph; spiroketal-amines such as spiroxam; hydroxyanilides such as fenhexamid; aminopyrazolones such as fenacet; thiocarbamates such as chlorfenapyr; allylamines such as naftifine and terbinafine, and mixtures thereof.

[0138] In one embodiment, the cell wall biosynthesis inhibitor fungicide may be selected from the group consisting of peptidyl pyrimidine nucleoside fungicides such as polyoxins, cinnamic acid amides such as dimethomorph, flumorph, pyrimorph; valinamide carbamates such as benthiafam, propineb, paraxamidine; mandelic acid amides such as mandelic acid amide and mixtures thereof.

[0139] In one embodiment, the melanin synthesis inhibitor fungicide may be selected from isobenzofuranones such as pyroquinolinones such as pyroquinolone; triazolylbenzothiazoles such as tricyclazole; cyclopropane-carboxamides such as cyproconazole; carboxamides such as diclofenac; propionamides such as cyproconazole; trifluoroethylcarbamates such as trifluoromethocarb; and mixtures thereof.

[0140] In one embodiment, the host plant defense inducer fungicide may be selected from benzothiadiazoles such as acibenzolar-S-methyl; benzisothiazoles such as thiabendazole; thiadiazole-carboxamides such as thiazolamide, isothiazolamide; polysaccharides such as laminarin; and mixtures thereof.

[0141] In another embodiment, the ergosterol biosynthesis inhibitor may be selected from prothioconazole, tebuconazole, hexaconazole, cyproconazole or epoxiconazole.

[0142] In one embodiment, the systemic fungicide may be a quinone exo- (Qo) inhibitor fungicide selected from the group consisting of azoxystrobin, syringystrobin, enoxastrobin, flutoxistrobin, picoxystrobin, pyraclostrobin, mandestrobin, pyraclostrobin, pyraclostrobin, chlorpyrifos-methyl, kresoxim-methyl, diastereamide, enoxastrobin, trifloxystrobin, famoxadone, fluoxastrobin, fenamidone, pyraclostrobin, and mixtures thereof.

[0143] In one embodiment, the quinone exo (Qo) inhibitor fungicide may be selected from azoxystrobin, picoxystrobin, kresoxim-methyl, pyraclostrobin and trifloxystrobin.

[0144] In one embodiment, the third systemic fungicide is selected from the group consisting of an external quinone inhibitor, an internal quinone inhibitor, a demethylation inhibitor, and / or a succinate dehydrogenase inhibitor; wherein:

[0145] (i) the quinone external inhibitor is selected from the group consisting of fenamidone, famoxadone and strobilurin fungicides, and the strobilurin fungicides are selected from the group consisting of azoxystrobin, mandestrobin, syringosterone, enoxastrobin, flutoxifen, pyraclostrobin, kresoxim-methyl, vinstrobin, fluoxastrobin, kresoxim-methyl, fenoxystrobin, orysastrobin, picoxystrobin, pyraclostrobin, chlorpyrifos-methyl, enoxastrobin, pyraclostrobin and trifloxystrobin;

[0146] (ii) the demethylation inhibitor is selected from triflumizole, triflumuron, triflumuron, triflumuronol, triflumuronol and conazole fungicides, and the conazole fungicides are selected from climbazole, clotrimazole, imazalil, oxaconazole, prochloraz, prochloraz manganese salt, triflumizole, azaconazole, bifenthionol, cyproconazole, benzyltriazole, difenoconazole, diniconazole, diniconazole-M, fluepiconazole, ethiconazole, fenbuconazole, trifluconazole, fluquinconazole, flusilazole, flutriafol, furconazole, furoconazole, hexaconazole, amide, ipconazole, metconazole, myclobutanil, tebuconazole, propiconazole, prothioconazole, quinazole, silyfenazole, tebuconazole, flufenconazole, triadimefon, triadimenol, triticonazole, uniconazole, pyraclostrobin and uniconazole-P;

[0147] (iii) the intraquinone inhibitor is selected from cyazofamid and indazofamid; and

[0148] (iv) The succinate dehydrogenase inhibitor is selected from the group consisting of flutolanil, flutolanil, mefenacet, fluopyram, furazolidone, carboxin, oxycarboxin, thiofuran, bixafen, flupyrad, furazolidone, pyraclostrobin, fluopyram, penthiopyrad, flutolanil and boscalid.

[0149] In one embodiment, the present invention provides the use of a fungicidal combination comprising one or more multi-site fungicides for controlling fungal infestations in soybean cultivation.

[0150] In a preferred embodiment, the present invention provides the use of a fungicidal combination comprising at least two multi-site fungicides selected from copper or its salts or sulfur for controlling the growth of phytopathogenic fungi in soybean cultivation.

[0151] In one embodiment, the present invention provides a fungicidal combination comprising one or more multi-site fungicides for controlling plant pathogenic fungi in soybean cultivation, for controlling plant pathogenic fungal diseases in soybean cultivation, these plant pathogenic fungal diseases include Asian soybean rust (ASR) caused by Phakopsora pachyrhizi, powdery mildew caused by Erysiphe disseminated, gray leaf spot (FLS) caused by the fungus Cercospora sojae, brown spot caused by Septoria sojae, purple leaf spot caused by Cercospora kikuchii, black pox caused by Elsinoeglycines, stem and pod blight caused by Diaporthephaseolorum var. sojae, and several other diseases.

[0152] In one embodiment, the present invention provides the use of a fungicidal combination for controlling plant pathogenic fungi in soybean cultivation, the combination comprising a first multi-site fungicide selected from copper compounds and a second multi-site fungicidal compound selected from sulfur, wherein the weight ratio of the copper compound to the sulfur ranges from 1:1 to 1:15.

[0153] In a preferred embodiment, the present invention provides a use of a fungicidal combination for controlling plant pathogenic fungi in soybean cultivation, wherein the combination comprises a first multi-site fungicide selected from copper compounds and a second multi-site fungicidal compound selected from sulfur, wherein the weight ratio of the copper compound to the sulfur ranges from 1:1 to 1:10.

[0154] In a more preferred embodiment, the present invention provides use of a fungicidal combination for controlling plant pathogenic fungi in soybean cultivation, the combination comprising a first multi-site fungicide selected from copper compounds and a second multi-site fungicidal compound selected from sulfur, wherein the weight ratio of the copper compound to the sulfur is 1:8.

[0155] In one embodiment, the present invention provides a use of a fungicidal combination for controlling plant pathogenic fungi in soybean cultivation, the combination comprising copper or a salt thereof and a second multi-site fungicidal compound selected from sulfur, wherein the weight ratio of copper or a salt thereof to sulfur ranges from 1:1 to 1:15.

[0156] In one embodiment, the present invention provides a use of a fungicidal combination for controlling plant pathogenic fungi in soybean cultivation, the combination comprising copper or a salt thereof and a second multi-site fungicidal compound selected from sulfur, wherein the weight ratio of copper or a salt thereof to sulfur ranges from 1:1 to 1:10.

[0157] In one embodiment, the present invention provides a use of a fungicidal combination for controlling plant pathogenic fungi in soybean cultivation, the combination comprising copper or a salt thereof and a second multi-site fungicidal compound selected from sulfur, wherein the weight ratio of copper or a salt thereof to sulfur is 1:8.

[0158] In one embodiment, the present invention provides use of a fungicidal combination for controlling plant pathogenic fungi in soybean cultivation, the combination comprising tribasic copper sulfate and sulfur, wherein the weight ratio of tribasic copper sulfate to sulfur ranges from 1:1 to 1:15.

[0159] In one embodiment, the present invention provides use of a fungicidal combination for controlling plant pathogenic fungi in soybean cultivation, the combination comprising tribasic copper sulfate and sulfur, wherein the weight ratio of tribasic copper sulfate to sulfur ranges from 1:1 to 1:10.

[0160] In one embodiment, the present invention provides a use of a fungicidal combination for controlling plant pathogenic fungi in soybean cultivation, the combination comprising tribasic copper sulfate and sulfur, wherein the weight ratio of tribasic copper sulfate to sulfur is in the range of 1:8.

[0161] In one embodiment, the present invention provides a fungicidal composition for controlling plant pathogenic fungi in soybean cultivation, the fungicidal composition comprising one or more multi-site fungicides and one or more agrochemically acceptable excipients.

[0162] In one embodiment, the present invention provides a fungicidal composition for controlling phytopathogenic fungi in soybean cultivation, the fungicidal composition comprising at least two multi-site fungicides and at least one agrochemically acceptable excipient.

[0163] In a preferred embodiment, the present invention provides a fungicidal composition comprising:

[0164] (i) a first multi-site fungicide selected from copper compounds,

[0165] (ii) a second multi-site fungicidal compound selected from sulfur, and

[0166] (iii) at least one agrochemically acceptable excipient.

[0167] In one embodiment, the present invention provides a fungicidal composition for controlling plant pathogenic fungi in soybean cultivation, the fungicidal composition comprising at least two multi-site fungicides selected from copper or its salts and sulfur, and at least one agrochemically acceptable excipient.

[0168] In one embodiment, the present invention provides a fungicidal composition for controlling plant pathogenic fungi in soybean cultivation, the composition comprising at least two multi-site fungicides selected from copper or its salts and sulfur, wherein the concentration of copper or its salts ranges from 1 g / kg to 200 g / kg, and the concentration of sulfur ranges from 100 g / kg to 800 g / kg.

[0169] In one embodiment, the present invention provides a fungicidal composition for controlling plant pathogenic fungi in soybean cultivation, the composition comprising at least two multi-site fungicides selected from copper or its salts and sulfur, wherein the concentration of copper or its salts ranges from 10 g / kg to 100 g / kg, and the concentration of sulfur ranges from 200 g / kg to 700 g / kg.

[0170] In one embodiment, the present invention provides a fungicidal composition for controlling plant pathogenic fungi in soybean cultivation, the composition comprising at least two multi-site fungicides selected from copper or its salts and sulfur, wherein the concentration of copper or its salts is 80 g / kg and the concentration of sulfur is 640 g / kg.

[0171] In one embodiment, the present invention provides a fungicidal composition for controlling plant pathogenic fungi in soybean cultivation, the composition comprising at least two multi-site fungicides selected from tribasic copper sulfate and sulfur, wherein the concentration of tribasic copper sulfate is 80 g / kg and the concentration of sulfur is 640 g / kg.

[0172] In one embodiment, the present invention provides a fungicidal composition for controlling plant pathogenic fungi in soybean cultivation, the fungicidal composition comprising at least two multi-site fungicides selected from copper or its salts and dithiocarbamate fungicides.

[0173] In one embodiment, the present invention provides a fungicidal composition for controlling plant pathogenic fungi in soybean cultivation, the fungicidal composition comprising at least two multi-site fungicides selected from tribasic copper sulfate and mancozeb.

[0174] In one embodiment, the present invention provides a fungicidal composition for controlling plant pathogenic fungi in soybean cultivation, the fungicidal composition comprising at least two multi-site fungicides selected from sulfur and dithiocarbamates.

[0175] In one embodiment, the present invention provides a fungicidal composition for controlling plant pathogenic fungi in soybean cultivation, the fungicidal composition comprising at least two multi-site fungicides selected from sulfur and mancozeb.

[0176] In a preferred embodiment, the present invention provides a fungicidal composition comprising:

[0177] (i) a first multi-site fungicide selected from copper compounds,

[0178] (ii) a second multi-site fungicidal compound selected from sulfur, and

[0179] (iii) agriculturally acceptable excipients.

[0180] In one embodiment, the composition of the present invention can generally be produced in the following manner: the active substance in the composition is mixed with an inert carrier, and surfactants and other adjuvants and carriers are added as needed and formulated into solid or liquid formulations, including but not limited to wettable powders, granules, dusts, soluble (liquid) concentrates, concentrated suspensions, oil-in-water emulsions, water-in-oil emulsions, emulsifiable concentrates, capsule suspensions, ZC formulations, oil dispersions or other known formulation types. The composition can also be used to process plant propagation materials, such as seeds, etc. The combination of the present disclosure can be prepared in the form of a composition.

[0181] Examples of solid carriers used in the formulations include fine powders or granules, such as minerals such as kaolin, attapulgite clay, bentonite, montmorillonite, acid white clay, pyrophyllite, talc, diatomaceous earth and calcite; natural organic materials such as corn cob meal and walnut shell meal; synthetic organic materials such as urea; salts such as calcium carbonate and ammonium sulfate; synthetic inorganic materials such as synthetic hydrated silica; and aromatic hydrocarbons such as xylene, alkylbenzenes and methylnaphthalene as liquid carriers; alcohols such as 2-propanol, ethylene glycol, propylene glycol and ethylene glycol monoethyl ether; ketones such as acetone, cyclohexanone and isophorone; vegetable oils such as soybean oil and cottonseed oil; petroleum aliphatic hydrocarbons, esters, dimethyl sulfoxide, acetonitrile and water.

[0182] Examples of the surfactant include anionic surfactants such as alkyl sulfate salts, alkyl aryl sulfonates, dialkyl sulfosuccinates, polyoxyethylene alkyl aryl ether phosphate salts, lignin sulfonates, and naphthalene sulfonate formaldehyde condensates; and nonionic surfactants such as polyoxyethylene alkyl aryl ether, polyoxyethylene alkyl polyoxypropylene block copolymers, and sorbitan fatty acid esters; and cationic surfactants such as alkyl trimethyl ammonium salts.

[0183] Examples of other formulation adjuvants include water-soluble polymers such as polyvinyl alcohol and polyvinyl pyrrolidone, polysaccharides such as gum arabic, alginic acid and its salts, CMC (carboxymethyl cellulose), xanthan gum, inorganic materials such as magnesium aluminum silicate and alumina sol, preservatives, colorants and stabilizers such as PAP (isopropyl acid phosphate) and BHT.

[0184] In one embodiment, the composition may further comprise one or more of an antifreeze agent, a wetting agent, a filler, a surfactant, an anti-caking agent, a pH adjuster, a preservative, a biocide, an antifoaming agent, a colorant, and other formulation aids.

[0185] Suitable antifreeze agents which may be added to the agrochemical compositions are liquid polyols, such as ethylene glycol, propylene glycol or glycerol.

[0186] Wetting agents that can be added to the agricultural chemical composition of the present invention include, but are not limited to, polyaryl alkoxylated phosphates and their potassium salts (e.g., Soprophor ® FLK, Stepfac TSP PE- K). Other suitable wetting agents include sodium dioctyl sulfosuccinate (e.g., Geropon ® SDS, Aerosol ® OT) and ethoxylated alcohols (e.g. Trideth-6; Rhodasurf ® BC 610; Tersperse ® 4894).

[0187] Optionally, from about 0.1% to about 5.0% by weight of an antifoaming agent or defoaming agent is used to prevent any unwanted foaming while producing a high concentration of the liquid biocide dispersion composition. Preferred antifoaming agents are selected from silicone-based compounds, alcohols, glycol ethers, mineral solvents, acetylene glycols, polysiloxanes, organosiloxanes, siloxane diols, reaction products of silicon dioxide and organosiloxane polymers, polydimethylsiloxanes, or polyalkylene glycols, either alone or in combination. Suitable defoaming agents include SAG-10, SAG-1000AP, SAG-1529, SAG-1538, SAG-1571, SAG-1572, SAG-1575, SAG-2 001, SAG-220, SAG-290, SAG-30, SAG-30E, SAG-330, SAG-47, SAG-5440, SAG-7133 and SAG-770.

[0188] Examples of thickeners based on anionic heteropolysaccharides from the xanthan group are, inter alia, Rhodopol 23 ® 、Rhodopol G ® , Rhodopol 50 MD® 、Rhodicare T ® Kelzan ® 、Kelzan S ® and Satiaxane CX91 ® .

[0189] Preservatives used can be benzisothiazolinone (Proxel GXL) or phenols, 2-bromo-2-nitropropane-1,3-diol (Bioban BP 30), 5-chloro-2-methyl-4-isothiazolin-3-one and 2-methyl-4-isothiazolin-3-one (KathonCG / ICP), glutaraldehyde (Ucarcide 50), chloromethylisothiazolinone (CMIT) / methylisothiazolinone (MIT) (Isocil Ultra 1.5), 2,2-dibromo-3-nitropropionamide (Reputain 20), natamycin and nisin, bronopol / CMIT / MIT (Mergal 721K3).

[0190] Suitable colorants (eg red, blue and green) are preferably slightly water-soluble pigments and water-soluble dyes. Examples are inorganic colorants (eg iron oxide, titanium oxide and iron hexacyanoferrate) and organic colorants (eg madder pigment, azo and phthalocyanine colorants).

[0191] The filler may include an organic or inorganic solid inert substance, such as talc, clay, diatomaceous earth, magnesium aluminum silicate, white carbon black, pyrophyllite, light calcium carbonate, high clay, organic bentonite, etc., or a mixture thereof.

[0192] The compositions of the present invention can be applied simultaneously as a tank mix or formulation, or they can be applied sequentially. Applications can be made to the soil before plant emergence (before or after planting). Applications can be made as foliar sprays at various times during crop development, with applications after emergence being made once or twice in the morning and evening.

[0193] The composition according to the invention can be applied before or after the useful plants or their propagation material are infected with the fungus.

[0194] In one embodiment, fungal diseases in soybean cultivation include Asian soybean rust (ASR) caused by Phakopsora pachyrhizi, powdery mildew caused by Erysiphe graminis, gray leaf spot (FLS) caused by the fungus Cercospora glycines, brown leaf spot caused by Septoria glycines, and several other diseases.

[0195] In one embodiment, the method of the present invention for controlling fungal plant pathogens comprises applying one or more multi-site fungicides to plants, plant parts, loci, or propagation material in a soybean cultivation.

[0196] In one embodiment, the method of the present invention for controlling fungal plant pathogens comprises applying at least two multi-site fungicides to plants, plant parts, loci, or propagation material in a soybean cultivation.

[0197] In one embodiment, the multi-site fungicide is selected from the group consisting of copper compounds, sulfur, dithiocarbamates, phthalimides, chloronitriles, sulfonamides, biguanides, triazines, quinones, quinoxalines, maleimides, and thiocarbamates.

[0198] In a preferred embodiment, the multi-site fungicide is a copper compound.

[0199] In one embodiment, the copper compound is an inorganic copper compound.

[0200] In one embodiment, the copper compound is a copper salt.

[0201] In one embodiment, the copper compound is a copper salt selected from copper sulfate, tribasic copper sulfate, dibasic copper sulfate, copper oxychloride, copper chloride, copper oxide, copper oxide, copper nitrate, copper perchlorate, copper bromide, copper hydroxide, copper iodide, copper acetate, and copper sulfate pentahydrate.

[0202] In a preferred embodiment, the copper compound is tribasic copper sulfate.

[0203] In one embodiment, the copper compound is copper sulfate.

[0204] In a preferred embodiment, the multi-site fungicide is sulfur.

[0205] In one embodiment, the method of the present invention for controlling fungal plant pathogens comprises applying at least two multi-site fungicides to plants, plant parts, loci, or propagation material in a soybean planting, wherein the first multi-site fungicide is selected from copper compounds and the second multi-site fungicidal compound is selected from sulfur.

[0206] In one embodiment, the present invention provides a method for controlling fungal plant pathogens comprising applying a combination of a multi-site fungicide comprising copper sulfate and sulfur to plants, plant parts, loci, or propagation material in a soybean cultivation.

[0207] In one embodiment, the present invention provides a method for controlling fungal plant pathogens comprising applying a multi-site fungicide combination comprising copper oxychloride and sulfur to plants, plant parts, loci, or propagation material in a soybean cultivation.

[0208] In a preferred embodiment, the present invention provides a method for controlling fungal plant pathogens comprising applying a multi-site fungicide combination comprising tribasic copper sulfate and sulfur to plants, plant parts, loci or propagation material in a soybean cultivation.

[0209] In a preferred embodiment, the present invention provides a method for controlling fungal plant pathogens, the method comprising applying a combination of a multi-site fungicide comprising copper or a salt thereof and sulfur to plants, plant parts, loci or propagation material under cultivation of soybeans at an application rate ranging from 0.01 L / ha to 200 L / ha.

[0210] In a preferred embodiment, the present invention provides a method for controlling fungal plant pathogens, the method comprising applying a combination of a multi-site fungicide comprising copper or a salt thereof and sulfur to plants, plant parts, loci or propagation material under cultivation of soybeans at an application rate ranging from 0.05 L / ha to 20 L / ha.

[0211] In a preferred embodiment, the present invention provides a method for controlling fungal plant pathogens, the method comprising applying a combination of a multi-site fungicide comprising a copper compound and sulfur to plants, plant parts, loci or propagation material under cultivation of soybeans at an application rate in the range of 0.1 L / ha to 10 L / ha.

[0212] In a preferred embodiment, the present invention provides a method for controlling fungal plant pathogens, the method comprising applying a combination of a multi-site fungicide comprising tribasic copper sulfate and sulfur to plants, plant parts, loci or propagation material under cultivation of soybeans at an application rate ranging from 0.1 L / ha to 10 L / ha.

[0213] In a preferred embodiment, the method of the present invention for controlling fungal plant pathogens comprises applying one or more multi-site fungicides comprising applying a combination of tribasic copper sulfate and sulfur to plants, plant parts, loci or propagation material in a soybean planting at an application rate ranging from 0.5 L / ha to 5 L / ha.

[0214] In a preferred embodiment, the method of the present invention for controlling fungal plant pathogens comprises applying one or more multi-site fungicides comprising applying a combination of tribasic copper sulfate and sulfur to plants, plant parts, loci or propagation material in a soybean planting at an application rate ranging from 0.5 L / ha to 3 L / ha.

[0215] In one embodiment, the present invention provides a method for controlling fungal plant pathogens, the method comprising applying a combination of a multi-site fungicide comprising copper or a salt thereof and sulfur to plants, plant parts, loci or propagation material under cultivation of soybeans, wherein the copper or a salt thereof is applied in an amount ranging from 100 g ai / ha to 5000 g ai / ha and the sulfur is applied in an amount ranging from 10 g ai / ha to 500 g ai / ha.

[0216] In a preferred embodiment, the present invention provides a method for controlling fungal plant pathogens, the method comprising applying a combination of a multi-site fungicide comprising copper or a salt thereof and sulfur to plants, plant parts, loci or propagation material under cultivation of soybeans, wherein the application rate of the copper or a salt thereof is applied in an amount ranging from 500 g ai / ha to 2000 g ai / ha and the sulfur is applied in an amount ranging from 50 g ai / ha to 300 g ai / ha.

[0217] In one embodiment, the present invention provides a method for controlling fungal plant pathogens, the method comprising applying a combination of a multi-site fungicide comprising tribasic copper sulfate and sulfur to plants, plant parts, loci or propagation material in a soybean planting, wherein the tribasic copper sulfate is applied in an amount ranging from 100 g ai / ha to 5000 g ai / ha and the sulfur is applied in an amount ranging from 10 g ai / ha to 500 g ai / ha.

[0218] In a preferred embodiment, the present invention provides a method for controlling fungal plant pathogens, the method comprising applying a combination of a multi-site fungicide comprising tribasic copper sulfate and sulfur to plants, plant parts, loci or propagation material in a soybean planting, wherein the tribasic copper sulfate is applied in an amount ranging from 500 g ai / ha to 2000 g ai / ha and the sulfur is applied in an amount ranging from 50 g ai / ha to 300 g ai / ha.

[0219] In a preferred embodiment, the present invention provides a method for controlling fungal plant pathogens, the method comprising applying a combination of a multi-site fungicide comprising copper or a salt thereof and sulfur to plants, plant parts, loci or propagation material in soybean cultivation, wherein the concentration of the copper or a salt thereof is in the range of 1 g / kg to 200 g / kg and the concentration of the sulfur is in the range of 100 g / kg to 800 g / kg.

[0220] In a preferred embodiment, the present invention provides a method for controlling fungal plant pathogens, the method comprising applying a combination of a multi-site fungicide comprising tribasic copper sulfate and sulfur to plants, plant parts, loci or propagation material in a soybean cultivation, wherein the concentration of copper or its salts ranges from 1 g / L to 200 g / L and the concentration of sulfur ranges from 100 g / L to 1000 g / L.

[0221] In a preferred embodiment, the present invention provides a method for controlling fungal plant pathogens, the method comprising applying a combination of a multi-site fungicide comprising tribasic copper sulfate and sulfur to plants, plant parts, loci or propagation material in a soybean cultivation, wherein the concentration of copper or its salts ranges from 10 g / kg to 100 g / kg and the concentration of sulfur ranges from 100 g / kg to 800 g / kg.

[0222] In a preferred embodiment, the present invention provides a method for controlling fungal plant pathogens, the method comprising applying a combination of a multi-site fungicide comprising tribasic copper sulfate and sulfur to plants, plant parts, loci or propagation material in a soybean cultivation, wherein the concentration of copper or its salts ranges from 20 g / kg to 80 g / kg and the concentration of sulfur ranges from 200 g / kg to 800 g / kg.

[0223] In a preferred embodiment, the present invention provides a method for controlling fungal plant pathogens, the method comprising applying a combination of a multi-site fungicide comprising tribasic copper sulfate and sulfur to plants, plant parts, loci or propagation material in a soybean cultivation, wherein the concentration of copper or its salts ranges from 40 g / kg to 60 g / kg and the concentration of sulfur ranges from 400 g / kg to 700 g / kg.

[0224] In a preferred embodiment, the present invention provides a method for controlling fungal plant pathogens, the method comprising applying a combination of a multi-site fungicide comprising tribasic copper sulfate and sulfur to plants, plant parts, loci or propagation material in a soybean planting, wherein the concentration of copper or a salt thereof is 80 g / kg and the concentration of sulfur is 640 g / kg.

[0225] In one embodiment, the method of controlling plant pathogenic fungal diseases of the present invention is applied to fungal diseases in soybean cultivation, including Asian soybean rust (ASR) caused by Puccinia pachyrhizi, powdery mildew caused by Erysiphe disseminated, gray leaf spot (FLS) caused by the fungus Cercospora sojae, brown spot caused by Septoria sojae, purple leaf spot caused by Psoralea corylifolia, black pox caused by Aspergillus sojae, stem and pod blight caused by Phaseolus vulgaris, and several other diseases.

[0226] In one embodiment, the methods of the present invention provide effective control of fungal pathogens, such that the methods can be applied before or after the emergence of fungal diseases, thereby providing resistance management and complete control of fungal pathogens.

[0227] In one embodiment, the methods of the present invention provide a method for effectively controlling fungal pathogens such that the method can be applied during the pre-harvest stage or the post-harvest stage of a crop.

[0228] In one embodiment, the compositions of the present invention may be combined with at least one additional agrochemical component / pesticide. Examples of such pesticides include, but are not limited to, herbicides, fungicides, acaricides, larvicides, avicides, insecticides, nematicides, and rodenticides.

[0229] In another embodiment, the method of the present invention further comprises applying a fungicidal compound, an insecticidal compound, or an herbicidal compound to the crop locus simultaneously, subsequently, or sequentially.

[0230] In one embodiment, the method of the present invention for controlling the growth of fungal pathogens is expanded for use as an eco-friendly, broad-spectrum product against pests.

[0231] According to one embodiment of the present disclosure, there is provided a kit comprising a plurality of components, each component comprising at least one ingredient of the disclosed fungicide combination.

[0232] In one embodiment of the present disclosure, the kit may include at least one of the components for preparing a fungicidal composition, or may include these components.

[0233] For example, the kit may include at least two multi-site fungicides and at least two systemic fungicides. One or more of these components may be combined together or pre-formulated within the kit. In those embodiments where more than two components are provided in the kit, the components may already be combined together and thus packaged in a single container, such as a vial, bottle, jar, pouch, bag, or canister.

[0234] In other embodiments, two or more components of the kit may be packaged separately, i.e., not pre-formulated. Thus, the kit may include one or more separate containers, such as vials, jars, bottles, sachets, bags, and / or canisters, each containing a separate component for the stabilized herbicidal composition.

[0235] In both forms, the individual components of the kit can be applied separately or together with the additional components, or as components of a combined composition for preparing the pesticidal combinations disclosed herein.

[0236] In view of the foregoing, it will be seen that several advantages of the present disclosure are achieved and other favorable results are obtained. Although the present disclosure has been fully disclosed, it should be understood that many additional modifications and variations may be made thereto without departing from the scope of the present disclosure. The embodiments may be combined together to better understand the present disclosure without departing from the scope of the present disclosure.

[0237] In another embodiment, the replacement or multiple embodiments of the disclosure disclosed herein should not be construed as limiting. Each embodiment may be mentioned and claimed individually or in any combination with other embodiments of the disclosure. Without departing from the scope of the disclosure, one or more embodiments of the disclosure may be combined to show the teachings of the present invention.

[0238] The present invention will now be described with reference to the following specific examples. It should be noted that the following examples illustrate rather than limit the present invention, and that those skilled in the art will be able to design many alternative embodiments without departing from the scope of the present invention.

[0239] It should be understood that this description and examples are illustrative rather than limiting of the present disclosure, and that those skilled in the art will recognize other embodiments within the spirit and scope of the present disclosure. Other embodiments that are also within the scope of the present disclosure can be practiced. The following examples illustrate the present disclosure but are not intended to limit the scope of the claims. DETAILED DESCRIPTION

[0240] Example

[0241] Example 1

[0242] The fungicidal activity of a combination of sulfur and tribasic copper sulfate was evaluated in soybean cultivation. Treatment 1 was a soybean crop untreated with the combination of the present invention, while Treatments 2 through 4 included applications of sulfur and tribasic copper sulfate. A total of six sprays were applied, each 10 days apart; the sprays were designated A, B, C, D, E, and F. Observations for fungal plant pathogen control were made 7 days after the last application of F (i.e., 7 days after the last application). The percent control of Phakopsora pachyrhizi and Powdery Mildew sphaeroides was determined.

[0243] Table 1: Treatment options

[0244]

[0245] SC: Suspension Concentrate

[0246] Table 2: Evaluation of % Control of Phakopsora pachyrhizi

[0247]

[0248] Table 3: Evaluation of % control of powdery mildew

[0249]

[0250] The % control of the fungal plant pathogens Erysiphe sphaeroides and Phakopsora pachyrhizi in soybean crops was found to be significantly higher, thus the combination of sulfur and tribasic copper sulfate is effective in controlling fungal diseases in soybean cultivation.

Claims

1. A method for controlling fungal diseases in soybean crops, the method comprising applying a fungicidal combination / composition comprising one or more multi-site fungicides to a plant, plant part, locus or propagation material.

2. The method of claim 1, wherein the multi-site fungicide comprises one or more inorganic fungicides.

3. The method according to claim 1, wherein the inorganic fungicide is selected from copper or its salts and sulfur.

4. The method of claim 1, wherein the copper salt is selected from the group consisting of copper sulfate, tribasic copper sulfate, dibasic copper sulfate, copper oxychloride, copper chloride, copper oxide, copper oxide, copper nitrate, copper perchlorate, copper bromide, copper hydroxide, copper iodide, copper acetate, and copper sulfate pentahydrate.

5. The method of claim 1, wherein the fungicidal combination comprises tribasic copper sulfate and sulfur.

6. The method of claim 1, wherein the ratio of the copper salt to sulfur ranges from 1:1 to 1:

15.

7. The method of claim 1, wherein the combination further comprises a systemic fungicide.

8. The method of claim 1, wherein the fungal diseases in soybean crops include Asian soybean rust (ASR) caused by Phakopsora pachyrhizi, powdery mildew caused by Erysiphe diffusa, gray leaf spot (FLS) caused by the fungus Cercospora sojina, brown spot caused by Septoria glycines, purple leaf spot caused by Cercospora kikuchii, black pox caused by Elsinoe glycines, and stem and pod blight caused by Diaporthe phaseolorum var. sojae.

9. The method of claim 1, wherein copper or its salts are applied in an amount ranging from 500 g ai / ha to 2000 g ai / ha and sulfur is applied in an amount ranging from 50 g ai / ha to 300 g ai / ha.

10. The method of claim 1, wherein the application rate ranges from 0.05 L / ha to 20 L / ha.

11. A fungicidal combination for controlling fungal diseases in soybean crops, the fungicidal combination comprising one or more multi-site fungicides, the combination comprising one or more inorganic fungicides selected from copper or its salts and sulfur.

12. The combination of claim 11, wherein the fungicidal combination comprises tribasic copper sulfate and sulfur.

13. The combination of claim 11, wherein the ratio of the copper salt to sulfur ranges from 1:1 to 1:

15.

14. A fungicidal composition for controlling fungal diseases in soybean crops, the composition comprising one or more multi-site fungicides and one or more agrochemically acceptable excipients, wherein the multi-site fungicide comprises one or more inorganic fungicides selected from copper or its salts and sulfur.

15. The composition according to claim 14, wherein the concentration of copper or its salt ranges from 10 g / kg to 100 g / kg, and the concentration of sulfur ranges from 200 g / kg to 700 g / kg.

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